LOCUS       DAZUKT010000001       100302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000001 DAZUKT010000000
VERSION     DAZUKT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.001"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG816_000001"
     CDS             591..1280
                     /locus_tag="ACG816_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4918925.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG816_000002"
     CDS             1267..2475
                     /locus_tag="ACG816_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4918926.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG816_000003"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG816_000003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4918927.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG816_000004"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG816_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4918928.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG816_000005"
     CDS             complement(6578..7273)
                     /locus_tag="ACG816_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4918929.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG816_000006"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG816_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4918930.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG816_000007"
     CDS             complement(8472..8663)
                     /locus_tag="ACG816_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4918931.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG816_000008"
     CDS             complement(8684..9574)
                     /locus_tag="ACG816_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4918932.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG816_000009"
     CDS             complement(9561..10328)
                     /locus_tag="ACG816_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4918933.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG816_000010"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG816_000010"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4918934.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG816_000011"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG816_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4918935.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG816_000012"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG816_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4918936.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG816_000013"
     CDS             14899..15282
                     /locus_tag="ACG816_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4918937.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG816_000014"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG816_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4918938.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG816_000015"
     CDS             complement(16226..17044)
                     /locus_tag="ACG816_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4918939.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG816_000016"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG816_000016"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4918940.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG816_000017"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG816_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4918941.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18173..19507
                     /gene="dnaA"
                     /locus_tag="ACG816_000018"
     CDS             18173..19507
                     /gene="dnaA"
                     /locus_tag="ACG816_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4918942.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19705..20835
                     /gene="dnaN"
                     /locus_tag="ACG816_000019"
     CDS             19705..20835
                     /gene="dnaN"
                     /locus_tag="ACG816_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4918943.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21064..21306
                     /gene="yaaA"
                     /locus_tag="ACG816_000020"
     CDS             21064..21306
                     /gene="yaaA"
                     /locus_tag="ACG816_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4918944.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21293..22417
                     /gene="recF"
                     /locus_tag="ACG816_000021"
     CDS             21293..22417
                     /gene="recF"
                     /locus_tag="ACG816_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4918945.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22414..24360
                     /gene="gyrB"
                     /locus_tag="ACG816_000022"
     CDS             22414..24360
                     /gene="gyrB"
                     /locus_tag="ACG816_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4918946.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24380..26851
                     /gene="gyrA"
                     /locus_tag="ACG816_000023"
     CDS             24380..26851
                     /gene="gyrA"
                     /locus_tag="ACG816_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4918947.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27087..27386
                     /gene="rpsF"
                     /locus_tag="ACG816_000024"
     CDS             27087..27386
                     /gene="rpsF"
                     /locus_tag="ACG816_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4918948.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27436..27948
                     /gene="ssb"
                     /locus_tag="ACG816_000025"
     CDS             27436..27948
                     /gene="ssb"
                     /locus_tag="ACG816_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4918949.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27974..28210
                     /gene="rpsR"
                     /locus_tag="ACG816_000026"
     CDS             27974..28210
                     /gene="rpsR"
                     /locus_tag="ACG816_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4918950.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28353..30326
                     /locus_tag="ACG816_000027"
     CDS             28353..30326
                     /locus_tag="ACG816_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4918951.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30332..30784
                     /gene="rplI"
                     /locus_tag="ACG816_000028"
     CDS             30332..30784
                     /gene="rplI"
                     /locus_tag="ACG816_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4918952.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31058..32425
                     /gene="dnaB"
                     /locus_tag="ACG816_000029"
     CDS             31058..32425
                     /gene="dnaB"
                     /locus_tag="ACG816_000029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4918953.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33205..34497
                     /locus_tag="ACG816_000030"
     CDS             33205..34497
                     /locus_tag="ACG816_000030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4918954.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35284..36147
                     /locus_tag="ACG816_000031"
     CDS             35284..36147
                     /locus_tag="ACG816_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4918955.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36276..37898)
                     /locus_tag="ACG816_000032"
     CDS             complement(36276..37898)
                     /locus_tag="ACG816_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4918956.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38147..38668
                     /locus_tag="ACG816_000033"
     CDS             38147..38668
                     /locus_tag="ACG816_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4918957.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38854..40584)
                     /gene="spxB"
                     /locus_tag="ACG816_000034"
     CDS             complement(38854..40584)
                     /gene="spxB"
                     /locus_tag="ACG816_000034"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4918958.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40749..41567
                     /locus_tag="ACG816_000035"
     CDS             40749..41567
                     /locus_tag="ACG816_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4918959.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41670..41867)
                     /locus_tag="ACG816_000036"
     CDS             complement(41670..41867)
                     /locus_tag="ACG816_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4918960.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42036..42614
                     /locus_tag="ACG816_000037"
     CDS             42036..42614
                     /locus_tag="ACG816_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4918961.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42709..42846
                     /locus_tag="ACG816_000038"
     CDS             42709..42846
                     /locus_tag="ACG816_000038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4918962.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43659..44162)
                     /locus_tag="ACG816_000039"
     CDS             complement(43659..44162)
                     /locus_tag="ACG816_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4918963.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44521..45546
                     /locus_tag="ACG816_000040"
     CDS             44521..45546
                     /locus_tag="ACG816_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4918964.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45869..46747
                     /locus_tag="ACG816_000041"
     CDS             45869..46747
                     /locus_tag="ACG816_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS4918965.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47223..47339
                     /locus_tag="ACG816_000042"
     CDS             47223..47339
                     /locus_tag="ACG816_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4918966.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48267..48605
                     /locus_tag="ACG816_000043"
     CDS             48267..48605
                     /locus_tag="ACG816_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4918967.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48699..48929
                     /locus_tag="ACG816_000044"
     CDS             48699..48929
                     /locus_tag="ACG816_000044"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4918968.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49329..49700
                     /locus_tag="ACG816_000045"
     CDS             49329..49700
                     /locus_tag="ACG816_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4918969.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49974..50666)
                     /locus_tag="ACG816_000046"
     CDS             complement(49974..50666)
                     /locus_tag="ACG816_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4918970.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50669..51454)
                     /locus_tag="ACG816_000047"
     CDS             complement(50669..51454)
                     /locus_tag="ACG816_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4918971.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51691..53091
                     /gene="lacG"
                     /locus_tag="ACG816_000048"
     CDS             51691..53091
                     /gene="lacG"
                     /locus_tag="ACG816_000048"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4918972.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53116..53433
                     /locus_tag="ACG816_000049"
     CDS             53116..53433
                     /locus_tag="ACG816_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4918973.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53446..55119
                     /locus_tag="ACG816_000050"
     CDS             53446..55119
                     /locus_tag="ACG816_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4918974.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55133..56764
                     /locus_tag="ACG816_000051"
     CDS             55133..56764
                     /locus_tag="ACG816_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4918975.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57255..58820)
                     /gene="guaA"
                     /locus_tag="ACG816_000052"
     CDS             complement(57255..58820)
                     /gene="guaA"
                     /locus_tag="ACG816_000052"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4918976.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58879..59802)
                     /gene="coaA"
                     /locus_tag="ACG816_000053"
     CDS             complement(58879..59802)
                     /gene="coaA"
                     /locus_tag="ACG816_000053"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4918977.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            59998..60603
                     /locus_tag="ACG816_000054"
     CDS             59998..60603
                     /locus_tag="ACG816_000054"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4918978.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60911..61927
                     /locus_tag="ACG816_000055"
     CDS             60911..61927
                     /locus_tag="ACG816_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4918979.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62068..63369
                     /locus_tag="ACG816_000056"
     CDS             62068..63369
                     /locus_tag="ACG816_000056"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4918980.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63415..64077
                     /gene="deoC"
                     /locus_tag="ACG816_000057"
     CDS             63415..64077
                     /gene="deoC"
                     /locus_tag="ACG816_000057"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4918981.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64098..64496
                     /locus_tag="ACG816_000058"
     CDS             64098..64496
                     /locus_tag="ACG816_000058"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4918982.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64562..65644
                     /locus_tag="ACG816_000059"
     CDS             64562..65644
                     /locus_tag="ACG816_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4918983.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65856..67418
                     /locus_tag="ACG816_000060"
     CDS             65856..67418
                     /locus_tag="ACG816_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4918984.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67411..68544
                     /locus_tag="ACG816_000061"
     CDS             67411..68544
                     /locus_tag="ACG816_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4918985.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68544..69500
                     /locus_tag="ACG816_000062"
     CDS             68544..69500
                     /locus_tag="ACG816_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4918986.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69599..70765
                     /gene="deoB"
                     /locus_tag="ACG816_000063"
     CDS             69599..70765
                     /gene="deoB"
                     /locus_tag="ACG816_000063"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4918987.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70789..71607
                     /locus_tag="ACG816_000064"
     CDS             70789..71607
                     /locus_tag="ACG816_000064"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4918988.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71624..72331
                     /gene="deoD"
                     /locus_tag="ACG816_000065"
     CDS             71624..72331
                     /gene="deoD"
                     /locus_tag="ACG816_000065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4918989.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72629..73315)
                     /gene="gpmA"
                     /locus_tag="ACG816_000066"
     CDS             complement(72629..73315)
                     /gene="gpmA"
                     /locus_tag="ACG816_000066"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4918990.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73487..74068)
                     /locus_tag="ACG816_000067"
     CDS             complement(73487..74068)
                     /locus_tag="ACG816_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4918991.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74201..74884)
                     /gene="rpiA"
                     /locus_tag="ACG816_000068"
     CDS             complement(74201..74884)
                     /gene="rpiA"
                     /locus_tag="ACG816_000068"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4918992.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75203..75616
                     /gene="rpsL"
                     /locus_tag="ACG816_000069"
     CDS             75203..75616
                     /gene="rpsL"
                     /locus_tag="ACG816_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4918993.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75700..76170
                     /gene="rpsG"
                     /locus_tag="ACG816_000070"
     CDS             75700..76170
                     /gene="rpsG"
                     /locus_tag="ACG816_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4918994.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76264..78351
                     /gene="fusA"
                     /locus_tag="ACG816_000071"
     CDS             76264..78351
                     /gene="fusA"
                     /locus_tag="ACG816_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4918995.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78523..79710
                     /gene="tuf"
                     /locus_tag="ACG816_000072"
     CDS             78523..79710
                     /gene="tuf"
                     /locus_tag="ACG816_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4918996.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80117..80425
                     /gene="rpsJ"
                     /locus_tag="ACG816_000073"
     CDS             80117..80425
                     /gene="rpsJ"
                     /locus_tag="ACG816_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4918997.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80456..81085
                     /gene="rplC"
                     /locus_tag="ACG816_000074"
     CDS             80456..81085
                     /gene="rplC"
                     /locus_tag="ACG816_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4918998.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81113..81736
                     /gene="rplD"
                     /locus_tag="ACG816_000075"
     CDS             81113..81736
                     /gene="rplD"
                     /locus_tag="ACG816_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4918999.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81736..82026
                     /gene="rplW"
                     /locus_tag="ACG816_000076"
     CDS             81736..82026
                     /gene="rplW"
                     /locus_tag="ACG816_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4919000.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82065..82898
                     /gene="rplB"
                     /locus_tag="ACG816_000077"
     CDS             82065..82898
                     /gene="rplB"
                     /locus_tag="ACG816_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4919001.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            82941..83219
                     /gene="rpsS"
                     /locus_tag="ACG816_000078"
     CDS             82941..83219
                     /gene="rpsS"
                     /locus_tag="ACG816_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4919002.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83242..83589
                     /gene="rplV"
                     /locus_tag="ACG816_000079"
     CDS             83242..83589
                     /gene="rplV"
                     /locus_tag="ACG816_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4919003.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83603..84259
                     /gene="rpsC"
                     /locus_tag="ACG816_000080"
     CDS             83603..84259
                     /gene="rpsC"
                     /locus_tag="ACG816_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4919004.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84262..84696
                     /gene="rplP"
                     /locus_tag="ACG816_000081"
     CDS             84262..84696
                     /gene="rplP"
                     /locus_tag="ACG816_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4919005.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84686..84874
                     /gene="rpmC"
                     /locus_tag="ACG816_000082"
     CDS             84686..84874
                     /gene="rpmC"
                     /locus_tag="ACG816_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4919006.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            84899..85165
                     /gene="rpsQ"
                     /locus_tag="ACG816_000083"
     CDS             84899..85165
                     /gene="rpsQ"
                     /locus_tag="ACG816_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4919007.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85223..85591
                     /gene="rplN"
                     /locus_tag="ACG816_000084"
     CDS             85223..85591
                     /gene="rplN"
                     /locus_tag="ACG816_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4919008.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85628..85936
                     /gene="rplX"
                     /locus_tag="ACG816_000085"
     CDS             85628..85936
                     /gene="rplX"
                     /locus_tag="ACG816_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4919009.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            85964..86503
                     /gene="rplE"
                     /locus_tag="ACG816_000086"
     CDS             85964..86503
                     /gene="rplE"
                     /locus_tag="ACG816_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4919010.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86522..86707
                     /locus_tag="ACG816_000087"
     CDS             86522..86707
                     /locus_tag="ACG816_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4919011.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86743..87141
                     /gene="rpsH"
                     /locus_tag="ACG816_000088"
     CDS             86743..87141
                     /gene="rpsH"
                     /locus_tag="ACG816_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4919012.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87173..87709
                     /gene="rplF"
                     /locus_tag="ACG816_000089"
     CDS             87173..87709
                     /gene="rplF"
                     /locus_tag="ACG816_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4919013.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            87870..88226
                     /gene="rplR"
                     /locus_tag="ACG816_000090"
     CDS             87870..88226
                     /gene="rplR"
                     /locus_tag="ACG816_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4919014.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88247..88747
                     /gene="rpsE"
                     /locus_tag="ACG816_000091"
     CDS             88247..88747
                     /gene="rpsE"
                     /locus_tag="ACG816_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4919015.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            88762..88941
                     /gene="rpmD"
                     /locus_tag="ACG816_000092"
     CDS             88762..88941
                     /gene="rpmD"
                     /locus_tag="ACG816_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4919016.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            88985..89425
                     /gene="rplO"
                     /locus_tag="ACG816_000093"
     CDS             88985..89425
                     /gene="rplO"
                     /locus_tag="ACG816_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4919017.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89425..90720
                     /gene="secY"
                     /locus_tag="ACG816_000094"
     CDS             89425..90720
                     /gene="secY"
                     /locus_tag="ACG816_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4919018.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            90782..91429
                     /locus_tag="ACG816_000095"
     CDS             90782..91429
                     /locus_tag="ACG816_000095"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4919019.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91620..91838
                     /gene="infA"
                     /locus_tag="ACG816_000096"
     CDS             91620..91838
                     /gene="infA"
                     /locus_tag="ACG816_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4919020.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            91871..91987
                     /gene="rpmJ"
                     /locus_tag="ACG816_000097"
     CDS             91871..91987
                     /gene="rpmJ"
                     /locus_tag="ACG816_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4919021.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92005..92370
                     /gene="rpsM"
                     /locus_tag="ACG816_000098"
     CDS             92005..92370
                     /gene="rpsM"
                     /locus_tag="ACG816_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4919022.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92398..92787
                     /gene="rpsK"
                     /locus_tag="ACG816_000099"
     CDS             92398..92787
                     /gene="rpsK"
                     /locus_tag="ACG816_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4919023.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            92867..93805
                     /locus_tag="ACG816_000100"
     CDS             92867..93805
                     /locus_tag="ACG816_000100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4919024.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            93833..94213
                     /gene="rplQ"
                     /locus_tag="ACG816_000101"
     CDS             93833..94213
                     /gene="rplQ"
                     /locus_tag="ACG816_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4919025.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94485..95324
                     /locus_tag="ACG816_000102"
     CDS             94485..95324
                     /locus_tag="ACG816_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4919026.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95300..96169
                     /locus_tag="ACG816_000103"
     CDS             95300..96169
                     /locus_tag="ACG816_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4919027.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96159..96956
                     /locus_tag="ACG816_000104"
     CDS             96159..96956
                     /locus_tag="ACG816_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4919028.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97159..97905
                     /gene="truA"
                     /locus_tag="ACG816_000105"
     CDS             97159..97905
                     /gene="truA"
                     /locus_tag="ACG816_000105"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4919029.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            98569..98763
                     /locus_tag="ACG816_000106"
                     /pseudo
     CDS             98569..98763
                     /locus_tag="ACG816_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(98951..99727)
                     /locus_tag="ACG816_000107"
     CDS             complement(98951..99727)
                     /locus_tag="ACG816_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4919030.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            99850..100257
                     /locus_tag="ACG816_000108"
     CDS             99850..100257
                     /locus_tag="ACG816_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFS4919031.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        31397 a        18445 c        20705 g        29755 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgcggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gagtcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa tggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggtgatttc ctgtgtattc cgtctcctaa ctattggtat atctatattt
    17941 tttatccaca gatgcaagag gaaatctatt gatttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    18061 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    18121 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    18181 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18241 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18301 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18361 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18421 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18481 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18541 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18601 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18661 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18721 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18781 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18841 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18901 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgcctcgttt ctcgctttgc
    18961 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    19021 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    19081 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    19141 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    19201 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19261 ttatcacatc caattgaaag atatcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19321 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19381 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19441 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19501 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19561 aaaagttatg cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19621 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19681 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19741 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19801 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19861 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19921 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19981 tttactttag aagttttaga aaataaacaa gtagcgatta cttctggaaa agcgaatttt
    20041 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    20101 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    20161 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    20221 ttattggctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20281 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20341 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20401 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatcctga taccaatcgt
    20461 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20521 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20581 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20641 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20701 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20761 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20821 gtacgtacaa attaacaatg attttcattg ttcttgaaaa tttaattgaa aagaaataga
    20881 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20941 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    21001 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    21061 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    21121 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    21181 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21241 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21301 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21361 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21421 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21481 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21541 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacaaaaaaa
    21601 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21661 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21721 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21781 tttaaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21841 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21901 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21961 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    22021 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    22081 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    22141 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    22201 taaagaagag acgggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22261 tgatagtcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22321 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22381 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22441 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22501 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22561 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22621 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22681 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22741 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22801 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22861 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22921 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22981 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    23041 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    23101 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    23161 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    23221 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23281 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23341 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23401 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23461 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23521 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23581 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23641 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23701 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23761 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23821 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23881 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23941 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    24001 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    24061 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    24121 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    24181 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24241 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24301 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24361 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24421 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24481 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24541 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24601 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24661 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24721 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24781 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24841 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24901 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24961 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    25021 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    25081 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    25141 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    25201 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25261 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25321 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25381 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25441 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25501 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25561 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25621 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25681 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25741 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25801 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25861 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25921 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25981 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    26041 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    26101 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    26161 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    26221 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26281 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26341 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26401 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26461 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26521 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26581 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26641 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26701 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26761 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26821 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26881 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26941 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    27001 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    27061 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    27121 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    27181 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27241 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27301 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27361 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27421 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27481 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27541 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27601 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27661 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27721 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27781 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27841 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27901 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27961 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    28021 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    28081 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    28141 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    28201 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28261 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28321 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28381 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28441 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28501 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28561 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28621 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28681 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28741 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28801 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28861 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28921 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28981 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    29041 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    29101 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    29161 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    29221 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29281 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29341 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29401 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29461 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29521 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29581 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29641 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29701 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29761 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29821 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29881 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29941 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    30001 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    30061 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    30121 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    30181 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30241 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30301 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30361 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30421 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30481 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30541 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30601 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30661 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30721 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30781 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30841 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30901 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30961 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    31021 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    31081 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    31141 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    31201 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31261 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31321 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31381 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31441 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31501 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31561 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31621 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31681 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31741 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31801 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31861 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31921 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31981 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    32041 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    32101 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    32161 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    32221 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32281 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32341 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32401 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32461 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32521 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32581 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32641 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32701 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32761 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32821 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32881 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32941 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    33001 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    33061 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    33121 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    33181 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33241 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33301 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33361 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33421 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33481 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33541 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33601 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33661 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33721 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33781 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33841 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33901 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33961 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    34021 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    34081 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    34141 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    34201 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34261 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34321 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34381 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34441 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34501 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34561 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34621 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34681 tttccgaaca aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa
    34741 aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca
    34801 caacctctcc actaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca
    34861 acaaacatga gaagctgttt tttgaaattg ttccttgtga ctcggagcta aacggcaggc
    34921 tcacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    34981 gcaaaatatg aggaactatt ttttccgttt ttttctctaa tactcaggtc aaaacgctga
    35041 tttcacaacc tctgattcac ggtgttccag aagtagcttc tacggaaata agccgaaatt
    35101 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
    35161 tctgtcacaa cctcttattt tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct
    35221 agcgcaagag atttgctata atagacgata agggtaaaat gtaacgagaa ggggttgaaa
    35281 aatgtgtatc aaattgtaac agattcttgt tgtgatctgc cataccatgt attagcaaaa
    35341 gaaaaagttg attttttatc aatgaatctc caattgaatg atcaagagtt agtagatgac
    35401 ctcggaaaga cattcaatta tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct
    35461 acgacatcac aaatcaatgt tggccgatat ttagaatttt tccgttctta tgcggaaaaa
    35521 cagattcctg ttttatatgt ggctttttcc agtggattaa gcggctctta cagtagtgcg
    35581 cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc ctgagatcta tgtttttgat
    35641 acaaaagctg caagtattgg ggaagggctt ttagtattgg aagctgtaag attgaaaaaa
    35701 gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa gtaattattt gaaagtccat
    35761 tcatgggtaa cagtagacga tctgaaatat ctagaacgag gcggtaggat ttccaaagca
    35821 agtgctactc ttggcagtct attgagtgta aagcctatta ttacggtgga taaagaagga
    35881 aggctgacga atgttgacaa agttcgagga agaaacaagt cgatccagac aatcgtcaat
    35941 gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg tttatgtggc ttacgctggg
    36001 gatctagaga gcgcggagaa ggcaaaacgg cttttggaag agcagataaa gatgaaagat
    36061 gtgaagcttt atcccttagg accaacgatc gccagtcaca caggctatgg ctgtatcgcg
    36121 attttttcga tgggaatcag tcgataaaag cagtaactca ttctgtaaaa aagcccagaa
    36181 attgattgta acaaaaacag cgactcttaa aagatagatg agcatcctat ctcttagagg
    36241 tcgctgtttt tttatttttt aagctaaata ggccattatg cccataactc gttcaattgt
    36301 ttttgaatat ctgcattttc caaaaattca tcatatgtcg tatcagctcg atcgatcaca
    36361 cctttatccg aaacggcaat gatgcggttt gccaatgttt ggataaattg atggtcatgt
    36421 gacgcaaata agattgagcc agtaaatgcc atcaagccgt cattcaatgc tgtgattgat
    36481 tctaagtcta aatggttcgt tggatcatct aatacaagta cgttagcttt cgaaagcatc
    36541 atttttgaaa gcatgcaacg gactttctct ccaccagata aaacattcac tggtttcaat
    36601 acttcttcac cagaaaacaa cattcttcct aagaagcttc ttaagaaggt gttatcatct
    36661 tcttctttac ttgcatattg acgcaaccag tctaaaatcg ttagatcagt atcgaattct
    36721 ttcgttgtat cctttggtaa ataagaatta cttgttgtca caccccaacg tactgttcca
    36781 gtatctggcg taatttcacc catgataact ttgaatagcg ttgttgttgt tatatcattt
    36841 tctgcgacga aagcgacctt gtcttctttg ctcaaattaa aagatatatc atctaaaatt
    36901 ttctttccat caatcgttac acttacattt tctacctgca ataagtcgtt tccaatctca
    36961 cgttcaggtt tgaatccaac gaatggataa cgtctagaag aaggttggat atcgtctaaa
    37021 gtaatttttt caagcatttt tttacgtgaa gttgcttgtt ttgattttga tgcattcgca
    37081 ctaaagcggg caataaattc ttggagttct ttgatttgtt cttctttttt ggcatttgcg
    37141 tttgcttgaa gtttagccgc tagttgacta gattccaacc aaaaatcata atttcctaca
    37201 taaagtttga ttttaccaaa atctagatcc gccatatgtg tacatacttt gttcaaaaag
    37261 tgacggtcat gggatacaac gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc
    37321 caattgatcg attggatatc cagaccgttt gttggttcgt ctagcaaaag aacatcaggt
    37381 ttaccaaata gtgtttgtgc taataacact tttacctttt gtccaccagt taattcactc
    37441 atttgctgat ggtgcaattc ttcagggatg ttcagacctt gcagtaaaac agctgcttct
    37501 ggttcagctt cccatccatt aagctcagca aattctcctt ctaattcagc tgctcgaata
    37561 ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat ctttttcttt cagcacttca
    37621 tacagacgct tgtgtcccat gatgactgtt tcaagtacag tatagtcttc atagtcgtaa
    37681 tggttctgtt tcaatacggc catccgttca tctggtccca tagtaacgga acctgtggaa
    37741 ggctgcagat cccctgataa cactttaagg aacgttgatt tacctgcacc atttgcacca
    37801 atcaagccat agcagtttcc gggggtaaat ttaatattca catcatcaaa tagtttgcgg
    37861 tctgaaaagt gcaaactcac atcatttaca gtaatcaatt tttttcctca cttttatcat
    37921 tattagtttt gacaagtctt atagaattat ataggggaaa ggtaaaagat tcaaggcaat
    37981 cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg
    38041 aaaatagcta ctataggatt tattagtttg caacagcata atttttgacg ttatttataa
    38101 gagggagtac tataaaactg taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag
    38161 aaccatcgac tggggctcat tgattttggg aattttattt gtacttgtct cattgttatc
    38221 atttcaagac cctgtcggaa atttagttgc aattgtagta gtctttgcga tttttgcctt
    38281 tattaaaggg atatttgaac tgtttgtacg gaacagatta aaagagctaa caggatataa
    38341 aggaaagatg cccttaataa tcgggatcat cgatattcta gttggcgtct ttttcttatt
    38401 caatattggt gcaggagtcg cagcattgcc ttttgttttt gcagtatggt ttattgctga
    38461 ttcagtttta gcgttattta ctgctgactt ggcaagagga gtaagtgaag gatattattg
    38521 gtttacgatc atcgtcaata ttttaggaat tttattagga attttcttat tgtttaatcc
    38581 aatttcttct gcgttgacat taagcttttt agttggattc tattttatgt tatttggtat
    38641 cacacatatc gtctatgcat ttagataagt aaaatcaaac aaataaaaaa gccggcttcc
    38701 gattacagtt ttactgttgt aattggaagc cggctttttt atcatggaca aatatgcacg
    38761 aggtgatttt taggtgtcga gacaaattta tgcaaaaatg tgagaagcta tatacacaaa
    38821 ttttatctta atactcgaag catagcgcag ttgtcacaac ctcttttcat ataattcatt
    38881 caatactggc aaatcatgga cttcatattt ttctttgaat gcttggattt cttcttcaga
    38941 atattttctt ggatctaaat gcagatcttc tactggcaat gggcgcttgt tattgatctt
    39001 cacatcgatt actacaggac gatctgattt tgcagctgca tcaaaggcaa atcgaagttg
    39061 cgagtattct gtgactgtaa agctatttgc cccgagcgct tctccaactt taccgaaatc
    39121 tgcatcttct aaaaatacac caaatttctt ttgttccgta tcttcttgtt ctgcttcgat
    39181 gaaaccaaaa gaatcattcg aaaagacaac attaataatt ggaagctgat atttgacttg
    39241 tgtaatgata tcttgcatga ccatagcaaa cccgccatca ccacttaagg taaatacttg
    39301 tttttctggg tagcttagtt gtgcggcaat tcctcctggc acaccattac ccatagttgc
    39361 gaaccatcca gatgtcgtat gtttttgttt accgttcata ttcaataaac gaatagaatg
    39421 gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat
    39481 ttctttatag acagcctctg gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa
    39541 ccaatttacc catttttctt tattttcttg atttgctagg taccagctat cagcagggcg
    39601 agcatcccct aactcgacaa gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa
    39661 agatgtttca tggcgtcgtc caaattttga cgcatcgata tcgatttgga taaattcagc
    39721 agttggatta aaaaatgcac gtccaaatgg gaagtcgcta ccgacaaata agatcagatc
    39781 ggcttctgcc aatgcttcat ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa
    39841 gttttcataa tcatcaggaa taattccttt tgctagaacg gaagcgacaa caggaactga
    39901 aaaatgttct gagaattctt tgatttgcgg aaatccgttt cgcgttcctt gaccaacata
    39961 aagaactggt ttttttgctt tttcaagata aggcaaagca gctagcaaat ctttttcttc
    40021 tggtaatatc acacttgttt tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc
    40081 ttcaaaacca agatcaacag ggatagtcac gatagccacg cctttatgct cataggcggc
    40141 tttaatggct tcatctacta catgcggaag actttgtggt gtcatgactg tacgattgta
    40201 tacgctaaca tctgcgaaaa taggattttc atttagttcc tggaaagagt tatagttcat
    40261 ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc acatggtcca tctgtgcatc
    40321 atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac
    40381 tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac
    40441 ttgaatgtag ttgatttcgg ttttttcttg atatagcgca tccataattg aattgaaaga
    40501 tcctccggga atgccgtaaa tatggtcgat accccagctt tcaagaactt ttaccattgc
    40561 gattcctgca ttgatttttg acatggtgtt tcactccttt agcaatgatg atacataaat
    40621 tttttgtatc tagagtatag aactttttat cgaaaatccc aactaattcg tcttttcaca
    40681 aaaatgtagt catgtttttg ggaatatgct agagtagtac atagatagat tcaagagagg
    40741 ggaatggcat ggttgaatgg ctattgagac ttgaaccttg gcagcaagca cttgctggga
    40801 cagcttttac ttattttatg acagctcttg gtgccgggtt ggtttttttc tttaaagaaa
    40861 tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc tggtgtcatg attgctgcaa
    40921 gtttttggtc tttgttagat ccggctatta caaaagcaga agaaaatgga gatattgctt
    40981 ggctagttgt aagtattggg tttggtttag gcggtgtgtt tttatatatg gcagataaaa
    41041 cactgcccca tatgcatttt ggaccacaac atgaagcaga aggattgcct acccatttaa
    41101 aacggacgat cttgcttgtt ttttctatta cattgcataa cattcctgaa ggtttagccg
    41161 tgggggtagc attcggtgca gcagcaactg cggataatcc gacagccgct atattagccg
    41221 ctgtttcggt ggcgctaggt attggtatcc aaaacttccc agaaggagca gctgtatcaa
    41281 tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt tgtttacgga caagcttctg
    41341 gaatcgttga accaattgct ggagtgattg gagcgctact agtaacgaaa gtggagctgc
    41401 tgcttcctta tgcacttgct tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat
    41461 tgatccctga agcacagcag acattgtcca gcaaacatca ttttgcagtt tttggcgtca
    41521 tgagtggatt tatcattatg atgattttgg atgtggcatt aggataaata aaaaaaatat
    41581 atatgatata tcaaataggg ccgcgaactt atatcgcgac cctatttttt tagtaagtta
    41641 tatcataatg aagtgaatgg tatcatacat taataatagt tttttctatg tgtatttcct
    41701 ttgatccagc gaacaagcca gatgataagt gcgattggga ttagaacttt gaccgcaaac
    41761 cataaaagcc cgcttaggac actgacaaaa agactgatga cgaagctagc tgcaattagc
    41821 actaaaagaa ccatgaagat atttaagaac atattttttt gacgcataca aaaccctcct
    41881 cttgttcatt ctttactata gaaaaagtat aacagggatt gtttaaaaac acctccaact
    41941 gaagactgat ttttacttca tctacttaaa tgatcaaaca attgcttttt ctctggttta
    42001 tcatctaaaa tagagcggaa aaggaggaga atatcatgcc taaaaatcgt cttgaagcat
    42061 ttacagatgc tgtaatcgcg attatcatga ctattttagt tttagaactt catgaaccta
    42121 acggagacac gtgggaggcg ctcgctgcat taggttaccg attttttatc tatattgtca
    42181 gttttatcat tttagcgatt tattggaaca atcatcatca tttatttcaa gttgtacata
    42241 agataaatgg acgggtctta tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc
    42301 cgtttgcaac ttcatggatc agtgagtaca tcaatagcct ggctccagaa atgacttatg
    42361 gttttgttac cttgaccgct aatattgcgt attatatctt ggcaaaagaa cttgtcaaag
    42421 caaataaagg ccacgagaaa gcattgaagg cgatgttgaa taattacaag aaatcgtatc
    42481 tatcgattgg tttgaatgtg ttaggaattg tcttagggta tttagtagcg ccagtagccg
    42541 tgctgatcgc aaacatcgtg attctagctg cttggatcat cccaagtcgt accatcgaat
    42601 cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa
    42661 taaacgatca aatatccgag tgatactagt attcaaagag ggatgcagat ggatcaaaaa
    42721 agaattcatg taggactgat tactagtttt gtcacgatgc ttatttattt ggatcaaggt
    42781 catttgactt ggacagtagt tggtgtcgta ggatttttga cattattcac agcaataaaa
    42841 aaataaacat ctttatgtaa tctgatgttc tatctattta agaaaagcat gtttacagca
    42901 gtatgattct gttgtaaacg tgtttttatt tgtggaaaat ttaattttta gaaaagtgta
    42961 tcgaaaaata gatagttagt ttttccacag tggataactt cgtaagagat aaaaataaaa
    43021 aattagtcaa agaagggcgt atagatttct tttgatagga gatatacaac agaaatgaat
    43081 aaatattaac gtgttttcaa acagtataaa acggtgtggt ttttggaata aaaaaataac
    43141 atatctcatt ttacactttt gttattttat acttaaaaaa taacgacaca gaaaataccg
    43201 tgtctcagat ttctctacta cttctaaaaa ctactgtttt tttcctttga aatgccttca
    43261 aaaagtagga tcaggcagaa tatactggca gaatcaagga gaagggagcc taaataagga
    43321 gaaaaaccat agaaaatata aagtaattac ttataaaaca agctttttat agaaaaataa
    43381 tatacctgag tagggtataa attaagacgg aattcctttc tagattgaga tattgactag
    43441 gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa ccctttttgt taaataaggg
    43501 gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct gcaataatat agtagattag
    43561 agatgaaaat agattttttt aaaccaatga ctgaataaaa agaccgtcat agatacacat
    43621 tggaatgtgt ctattacggt ctttttttac gaacgatttt attgaagacg gacagttcgg
    43681 gtcaaaaata aaaaagctac gatcagaaat aagaataaaa tgtatcccaa aataatggct
    43741 agacggtcta acttatttgt cgatagatgt ttgtttagaa gttgataggc ccataaacct
    43801 atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt ccgctacgcc aataccgaat
    43861 aggaattgaa tcaattctgc caagaagctt acaaggaaaa taatccctaa ttttttagaa
    43921 aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag
    43981 ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg
    44041 agattgacat tgctgtaaga ataagtgaga aattgtggaa gattaaatga tagcttgaat
    44101 aaaatcaacc ataatagaaa gaataaataa atccctaata aaaattttga tacataccgc
    44161 atagacatct cctccattta aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa
    44221 gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat tagaagcttt tttttgttgg
    44281 gaatccggtt tctttaacaa agctaagata gcgagttgct tggaaaagtt gctatctttt
    44341 ttctgtcaaa acgactcgga atgttcgttt tttgttctaa tttctccaag ttgtttactt
    44401 tttgatacta agtttatgaa aaatcaagaa atagttgtta tactaggtaa tgctgacaaa
    44461 cgggattggc atttcatctt ggttcagctt tttttccaat tactaggaag gagggataga
    44521 ttgttcaaga cttattttcg tttcaataaa atacaagata atccttttcg tatcatgaat
    44581 ctgatgctac ttatctttat tgtattatgg ttaggttaca taaatcatga ttttttgatt
    44641 tcaagttttt catcattagg gatctttact tttttctatt atcaaaacat accgatgaaa
    44701 tatttgctga aacgtatgtt tctgatcggt tgcggactgt tggttgcttt cgttttgggg
    44761 atcttatcga cttatgtttt atggcttgaa ccatttgccg tagctttggt tgctttttcc
    44821 agcagattcg tgttgcgatt attccatatt tctaaaccag gtggcttgtt ttttgcgatg
    44881 ctttctgcca tgggaacgag catgcagctg ccaatcgctc aattaccgat cgtctcactt
    44941 tattttttta tgggtgtcgt ttttgccctg attgcggcag tgattacaaa attactggat
    45001 tcacggcctg agcagacaat tgaaaaagca actttaaaag aaagatttca cgaagagcct
    45061 ttagtgatca ttgattctgt tttctacagc gctgccttat ttctttccgt ttatgtcagt
    45121 cacggcttga atctgcataa tccttattgg ttgaccttgt catgtgcatc gattttgcta
    45181 gcagaaaact tggatgcgat gaagcatcga caagtccaat atctgattgg atcgatgggt
    45241 gggttatgtg tgtcggcatt cttgtcattt gtgccgttta cgcagttgca gaccattttt
    45301 ctgatcactt ttctatatgg aatcgcacaa tttctagttg ctagaaatta tgcagttgct
    45361 aatatttttc ttaatccgat ggcgttgatg ctatctacac ttattcgtgg agcatacttg
    45421 atttctttga tcgaatatcg ttttttaggg atcgtcatag gaagtttcat cggtttaggt
    45481 gttgcgtggg taatgacggt tggtttgcaa cattatttaa cggttgttcg tcaaaaatta
    45541 gaatagaagg acaaatatat gtgccaaaag aggttatgac aagatttttg tcacaacctc
    45601 taattcaagg tgttcaaaag ccagattctc agcattaagt caaattcaca caaaaatgtg
    45661 gaaaggaact ttcaaaaaaa ttttatattt attctatcaa aaaattttgt ttatgatggt
    45721 aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac agatattctt
    45781 tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt tataccatat
    45841 ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa ggggaaattt
    45901 gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc ttcccaaagt
    45961 cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt ttgagcaata
    46021 tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa tagacaaagt
    46081 ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt ctcaaagttg
    46141 gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa caatgaatgc
    46201 ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct ttgaccgtgg
    46261 tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat tggagtacga
    46321 aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc agtgcctttc
    46381 tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata gttacgaaag
    46441 tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga tgtacacaga
    46501 tacttggtta ctatcaccca atcttactaa atggttgaaa aaagagtcaa aaatctgtct
    46561 ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg gagtcccttg
    46621 gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga gtttacagcg
    46681 acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag gtattcttac
    46741 ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt aaagaacact
    46801 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
    46861 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
    46921 ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag tacgttcaat
    46981 aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc ttttttaggt
    47041 agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag ctagtctgat
    47101 cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa acacaaatcg
    47161 taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag tgggtcgtcc
    47221 atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca aatatcaaaa
    47281 cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg aaaaggtaag
    47341 aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt ttcaataatc
    47401 ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta acatatgata
    47461 aattggtaga atctttcttt tcaaattgat accattccct aattccagtt atatagttaa
    47521 ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat ttttcaattc
    47581 cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa cacctttgtt
    47641 cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa aggttttgcg
    47701 aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat aaccattatc
    47761 gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac tccatttctt
    47821 ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt taactttaaa
    47881 aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag ttactattgc tagaataaca
    47941 tcaaggaata ataagttttg gttttcagat aatagtcgat aagttgtgta ttggaatttt
    48001 agatacagta ataaagtatt tttcatgaaa ttttgtgaga atcactatta caagtatctt
    48061 taataaaatt aatggttaag gaggaactta aagtagaatg taaatgaata gttgtagtca
    48121 gtcgattgtg atactagact caaagttgca ggaataagtt tttcaatttg tgattggaag
    48181 ataaaaaatg aaaaagaagg gctttttttt atactttgtt gattgtagtg tttattttcc
    48241 ctctgtttag ccaagtaaca ttttctatga ctcaaccagt ttcaagtgag gaacatgata
    48301 acctttttcg tgctacagaa gagtttaaga aatatcagga aaatattgtt ccggagactc
    48361 cattagctag tgagccaatt actgatattg atggaaacca aataggatgg tatatacagt
    48421 acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa tacagtaggc ttagagctaa
    48481 caccgattga aaaggtgagt tcattgttaa cgtatattta tactgatgaa agtgacgaga
    48541 ctgagatcat tttaacggat tactcaaaaa ttgctactga taaaaagtat atattgtgga
    48601 tgtagacact agtgaaatac tttatgatat agaaattgat gaaaatttac tagatgtagc
    48661 acaagaaatc gatcatcaaa agcaggaagc tattcgatat gctggtagaa aagggaaaag
    48721 aaaatattta ttacgtgaat gtagcaaaag ttagagaaga tgaaaatgag tggaaagaat
    48781 tcaagagtcg ctactcaatt aactcgactc cgacatttac agtttacaga gaaggatcga
    48841 tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc tttagctgaa gtagaagagt
    48901 ttttggatta tgttagtatg caacagtaag ggagttctta ttatgaaaaa ttttgacaca
    48961 tattatgtta ttgttttttc gatatctttg ctgttaatat tactaacttt aatgcagtat
    49021 aaagagtatc cagagtattt tattttaact tttgtatatt tagtaagtag ctacacactt
    49081 ctatataaat tcagaagttc ttctagagtt tctaaagttt gtttcttaat tagcttattg
    49141 attctataat atttttgatt tatataggaa attaaaaata ttatagaatc aacactcaga
    49201 gaacgggttg gtttttaata gatttaataa gtactgataa tgaaatttct cctggttggg
    49261 aaaacgaagt tggttaccct gtagaagaat tttttcaaga ttactttgcg gagtatgccg
    49321 ataggataat gagagattac gtaacagata aaactcaaga agtcatcaat caatcccagt
    49381 cattaaatga attatctgat aaggcgaaag aagaagcagt tgccattatt tcttcaggga
    49441 ttagtgacta tattgatatg agtggtgaaa tcaattatgg cgataaagac aaaattgtac
    49501 gcatgataaa agcttttatc aaaacttttg atgtgaaggc agatcttaag tttaatgata
    49561 cttacctagt aggaaaatta attaatatcc ttgggacaga gaaagatacg gaaacgttta
    49621 tttcctcttt gtctatgttg tttacagata agcatttttc aactcagctt ggtggagagg
    49681 aactagtgga aatcttttaa ccattacgcc cagcattaat aaaagtatat caaaaaagta
    49741 ctcctgatga tttttaagat tggtttgaga aataatgtga aaacactatt tttaatgtta
    49801 attatagata gagatgaacg cgtcatatgg ttaaaagaat gaaaaagtca aagatacact
    49861 gctatatttc actaaggata tagcagtgta tctttcatgt cgtttcactg gcagtttttt
    49921 ataaaaggag gtgtgcattt cagttgcttt tctttggtag ttagttgaat cgtttaagaa
    49981 ttaacggatg tggcagacag gtgctctgtc caaaaaccag taaaatcttt gatggttcca
    50041 tctttattta gctgtgcctg ttcaaatact gttttgtttt taccgaatgg atcaattgca
    50101 ataattgttc cgatccccgc acgtttggca gcaagtaatc ctgagtaagc atcttcgata
    50161 acaagacaat cagccggagc taagctcagt ttgtctgccg ctttaagaaa aatatccggt
    50221 tgcggtttcc cagggaaaga gccgtcgtca tagacaatct tgtcccagtc aaaccagcgg
    50281 gctaaatcga aaatatcaaa ataaaaagca acattctctt tgacagtagc cgtggcaatc
    50341 gttaatggaa tctgttggtc agtcagctgg tcaagagtcg cttcaagccc ttttgtcaac
    50401 tgaagctcgt tttcattttg caagcaaagc atacgataat agctttcttt ttcttctgac
    50461 aatacggcga tctcagaggc ggtcaaatgc tctgaaatga aatgtttcaa aatctccgta
    50521 ttcgtccgac catgaatatt ttttaaaata tcctcgtctg ataaaccatc ttttgtatga
    50581 tcatgtatca tatgcagcca agcagcttca tgcagatgag agtccaaaaa cattgttccg
    50641 ttgaaatcaa atataatccc tttcatagtt acgctcctat agtggtattt tcggttgaat
    50701 cagcaatttg cagatcgaca tattgtgcat agtgctcttg aacctcccgc gagatctggc
    50761 gatccgtaat gagcagatcg aaatcatgca aattataaaa cacataaaaa tcatctcgat
    50821 tgaatttttt attatcaacg agtagatatt tcttacggga attatttaga gcgatctgct
    50881 gtgcttctcc ttcttcggta ctgtaagtag agatcgcttc gttgtgaacg ccatttgaac
    50941 tgataaatgc ttttgtaaac tttagtcttc ttaaattgtc gttggtgatc ggtccgacaa
    51001 atgcgccggt atttttccgg aaatcaccgc caatcagtag aatttctggt ccatctgctt
    51061 gagcaagaat atcaaaaact ggataattat ttgtaatgat ccgaattttc ttgttcagta
    51121 aatgcgtcgc caatagttcg attgttgtac ctggtcctaa gaaaatcgtt tctccctcgt
    51181 gaatcaagga ggctgctgtg gcagcaattt cttttttttc ttgaatctgc agagtggctt
    51241 tttcagtgtg agaaagttct tgatctaaag tgtaggaaag actttgagcc cctccatgga
    51301 cacgcagcag cttgcccgat ttttctaatt catctaaatc tctgcgaacg gtcatatcag
    51361 aaacatctaa ttgattgatg atttcattaa cggtaaggat tcctttttta ttgaccattt
    51421 ctacgatagt aagcaatctt tcttttttca acattgtctt cacctctaat ttaaggataa
    51481 tctatcatat aaacggacaa cagtcaatct aattcgaaca aaagtaaaca tttagggtgg
    51541 tttttatgtt tgttacaata gattttattg ctgttttctg gtttttatga ttatttgtgt
    51601 ttgtttttgt tgacaaatgt tttggatgcg tttacaatgc aatcagttag aaagagcgaa
    51661 aactgattat agatttgagg agagatgtac atgcttaaat taccagaaga ttttattttt
    51721 ggcggagcca cagcagccta tcaggttgaa ggagccacta aggaaggtgg caaaggcgct
    51781 gttgcttggg atgacttttt agaggaacaa gggcgattca gtcccgatcc ggcaagtgat
    51841 ttttatcatc aatatgcaaa ggacattgaa ttgtgtgaac gttttggtgt aaatggtcta
    51901 agattatcca ttgcttggag ccgcattttt cctgatggag ctgggaaacc aaatccagaa
    51961 ggaatcgctt tttatcatcg tgtgttcgaa gaatgtaaga aaagaaacgt aacgccgttt
    52021 gtgacgttgc atcattttga tacccctaaa cgtttgtttg accatggaga ttttttgaat
    52081 cgagaaacaa tagaagcatt tgtttcctat gccatttttt gttttcatga attcaaagaa
    52141 gtcaaggtgt ggagtacctt taatgaaatc tatccagttg caaccaatca atatttgtta
    52201 ggtgttttcc caccaggaat caaatatgat tttacaaaga tcgtcgcctg tcttcataat
    52261 atgatggttg ctcatgcccg tgtagtgaac tatttcaaag aaaacgaact tccgggtgaa
    52321 attggtgtag ttcactcatt agagacgaaa tatgcggcaa cagacgcgcc ggaagataag
    52381 catgctgcct ttttagacga tgcattgtcg atccgtttct tattagatgc tacctattta
    52441 ggctattatt caactgaaac cttgactgct cttgatgaga tatgtgaagc aaatcaggcc
    52501 agctatcatt ttcctgaaga agatttcgtt gaattgaaaa aagcatccac gcgtaatgac
    52561 tacttaggga tcaatcacta ccaatgccat tttgtcaaag cttacgatgg agaaaatgca
    52621 atccatcata acggtacggg tgaaaaaggg acctctgttt ataaagtgaa gggaatcgga
    52681 gaacgtattt ataaagaagg aattcctcga actgattggg actggctgat ttatccagaa
    52741 ggtctatatg acttgttgct gcgaattaaa agcgattacc cacattacaa caaaatctat
    52801 atcactgaga acgggatggg gtataaagat cagtttgaag atggcattat tatggatcag
    52861 ccgagaatcg actatttgag agtctatttg gaatccttaa gcaaggcgat aacagccggg
    52921 gtaaatgtga aggggtattt tttgtggtcg cttatggatc ttttctcatg gacaaatggg
    52981 tataacaaaa ggtatggctt gttttatgtt gattttgaaa cccagaagcg ttacccaaaa
    53041 gaatcagctt actggtacaa gttggtcagt gaaaccaaaa cgattattta ataagtgata
    53101 atggaggaag aaaagatgaa taaagaggaa ttacaaatgc ttggttttga gatcgttgca
    53161 tattcaggcg acgctcgcag tacattattg aaattattga aagaagtaag acaaggaaac
    53221 tttgaacatg ttgattcggc gttgaaagaa gccgacgaaa atttaaccct tgcacataat
    53281 tcgcaaacaa aaattttagc ggaagaagca tctgggaaag agatggagat gggattcatt
    53341 tttatccatg ggcaggatca tttaatgacc actttattgc tgagagatct gattcaagac
    53401 tttattgtac tgtacagaca gaatcaagga taggagaaga acgctatgga tggaatcatt
    53461 aaacaaatcg aaaaagggaa accatttttt gaaaaaatct cacgtaatag ttatttagga
    53521 gccgttcgtg acggattttt aacagcgatg ccagctattt tattttcaag cattttcatt
    53581 ttgattgctg cggttcctga gatttttggt taccaatggc ctgaatctgt ctccacatgg
    53641 ctatggaaag tctataatta ttcgatgggt gttgtagggt tactcgtttc agcaaccact
    53701 gctcgctgct tggctgattc gatgaatcgt cgcttaccga aaaacaaagt aatcaacacc
    53761 acctctgtga tgctggcctc tgtggtaagt ttcctgttgt taagcgtctc tccgattgaa
    53821 ggtggatttt caacggaata tatggggaca aagggactac tggcttcctt tgtcgctgca
    53881 tttttaacag ttaatttata taagttttgt gtgctgaaag atattaccat taaaatgcct
    53941 aaagaagttc ctggaacgat ttcgcaaacc ttccgagata tttttccttt ctctttttca
    54001 gtatttgcgg ccgttttaat tgatttaggc attcgacaaa tcttccaaat ctcatttgca
    54061 gaagcaatca ttacggtgct gcagccgcta tttacagcag ctgacgggta cttaggaatc
    54121 gctattatct ggggagcgat ggcgctgttt tggtttgtcg gtgttcatgg accgtcgatc
    54181 gtggagccgg caattgcggc aattatctat gccaatgtgg aaaccaatct gcaattgttc
    54241 aattccggtg aacaagcttc taaagtactg acggtgggtt tagggaattt tgtaggaact
    54301 atgggaggaa ctggggcaac attggttgtt ccttttatct tcctactctt tgctaagtct
    54361 aagcagttga aggcagttgg gaaagcttcc tttattccag taagtttcgc tgtcaacgaa
    54421 ccattgctat ttgcggcacc catgattttg aacccttatt tttttattcc atttttgtta
    54481 acacctgttg taaacgtttg cctgttcaag ttttttgtcg atgtattggg catgaacagc
    54541 tttatgtacg ttttgccttg ggcaactcca gcaccgatcg gtttggttct aggaacgggc
    54601 atgggtatct tagcttttgt tctagcagcg gttctaatcg tcgttgactt cctgatctat
    54661 ctaccatttt gcaaagcata cgatgctgtc ttagtagaga aagaagcaca aaaagcagag
    54721 gtgatcgcaa ccgaggaagg tgcttctgcc gatgtacgtg aggaagtacc tgtggcagcg
    54781 agtcaagtta tcagcgatgc aaataaagaa atcaatgtgt tggtactttg tgctggcgca
    54841 gggacaagtg cgatgttagc taatgcttta aaaaaaggtg catctgaatc gaaagcaccg
    54901 atcagtgcaa tggcgggtgc ttatggttct cattacgaaa tgctgcagga ttttgatatg
    54961 gtggtcttag caccgcaagt caattcttac tatgatgata tcaaagaaga caccgatcgc
    55021 ttaggaatca agctggttgc gacaaaagga gcagaatata tcggattaac gagagatcct
    55081 gaaaaagctg ttcagtttat tctgaaccaa tttcaataaa ggaggagcca taatgaaaat
    55141 gggaatttgg ttgcgtaaag ctgttttatt tgcagctgtg ttaggggctt tgggggctgt
    55201 ttcaaaaact gttgaagcat ctgccagcga atttattcgc ggagcagata tttcgatcct
    55261 gtcagatatg gaaaaatctg gtgcaaaata ttatgaagac gatatttcaa aagatgcctt
    55321 agagatttta aaaaataatg gtgtcaacta tgttcgactg cgtttgtggc aggatcctta
    55381 taatagtaat ggagaaagtt atggagcagg gacaaatgat ctggaaacga cgattgacct
    55441 tgcaaaacga gcaaaaaatc tgggacttaa ggtgctgctg gattttcact acagtgattt
    55501 ttgggttgat cctgggaaac agaatcttcc taaagcatgg caaggtctga cctttgaaga
    55561 aatgaatacg gcattgtacg actatacaaa gaacgttctg tcggaaatga agcagctgga
    55621 cgtttatcct gatatggttc agatcggcaa tgaactgaat agcggaatgc tctggcctta
    55681 tggcaagtct tggggagaag gcgggggaga atttgatcga ttggctgctt ttttgaagtc
    55741 tggtatccaa gctgttcgag acactcaacc taaaacaact ccagtaatgc tccacttagc
    55801 tgatggtggc gatacaggag catttacttg gtggtttgat gaaatcacga gtcgaggtgt
    55861 gtcatttgat ttaattggag tgtcgtatta tccgtattgg cacggttcaa tgaatgaact
    55921 tcaaaccaat atgaatgcga tcagccaacg gtatggtaaa gaagtgatcg tagttgaaac
    55981 agcttatggg catacaacag cgaatgccga tactatgcca aatgcttttg gtgaagccga
    56041 agcagctgcg gggggatatg aaccaacacc gacaggacaa gcagagtatt tgctggatct
    56101 tgccgatcgg attcaggcag taccgaataa tcgcggggcg ggcttcttct attgggaacc
    56161 tctttggtac aacggaaatg tgagttgggc aacacaagca ggtatgaatt atctcagtgt
    56221 tcaatccact atgggaaacg aatgggacaa ccaagcgatg tttgattttg ctggaaacgc
    56281 cttgccaagc ttgcgtgcgt ttaaacaagc aggggcgcaa ataaatctag ttaaaaatgc
    56341 aagtttcgaa agcgatggac caacgaccag tccttccaat tggcaaacct ggtttcaaca
    56401 aggaaccgct aagggtacga tcaaaacgga agcgggcact ttttttgggg actacaaact
    56461 aactttttgg caggatcaag catacgaggc atcagcttat caaaatgtta gtggcataag
    56521 caaaggaact tacacattga gtgcctatgt aatgtctggg ggagagtttg atactaatca
    56581 gttatatatc aaaaattatg gtggagcgga actaaatact gcggttgtta aaacaagtga
    56641 atggacaaaa attacaatca aagacattcc aattacaaca ggtgtttgtg aaattggggt
    56701 ttatagcaag gcttcaggaa atgcttggac gaacgttgat tttgtttcat tggtgaaaca
    56761 atagactgcg gatcgtttcg attcaagatc ggaggttccc ttcagccaca tcgttagcgt
    56821 agaaggcagg aatcgaacct gcacctcgcc tttgccaaaa ataaccagaa acgatttcca
    56881 gtcattttta ccaaaacgag taaagcacca gacttcatct acactttgta ttatgctaaa
    56941 gagagttcaa cgatttcgta agaagcaatg aaaaaattgc aaaaagttgt gcagataaca
    57001 taaagtttgt ttaagaaagg gtaaccttag aattccgtcg tgaatatgat ttgagcactt
    57061 agatggatag aaaaaagttc tcggaaaaca gttcgttttt cgagaacttc tttttatgat
    57121 aagaatgaaa caaattaact tatattatag tctcttttgg tagggaacca tcttattttt
    57181 aatgaaaatc aagcatttta gctattgaag aatgttgatt tatagggatt ccccctccat
    57241 attatgttgg ctctttattc ccactccact gttgctggtg gtttagaagt gatgtcatac
    57301 acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt ttgcaagact
    57361 tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac agcacggata
    57421 ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg gatccctgga
    57481 agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat ttcttcacgc
    57541 aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc gccaagaaca
    57601 cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc aggcatgcct
    57661 aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat
    57721 tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat cgtctgtgct
    57781 gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag gaaagagacg
    57841 ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa ttcattccca
    57901 atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa gaagcgatct
    57961 cttgcattga ctttgatgat gttcaagccg aattttccgc ctagactttc cattacttgt
    58021 tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg
    58081 atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga aagaccaagc
    58141 aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc gataaagtta
    58201 tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg cagcaattcg
    58261 attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa attacgtgtt
    58321 gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc aaagccttca
    58381 ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt tggtgtttct
    58441 ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata ttcgcggttt
    58501 ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc atagcaaatc
    58561 cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc attctcatca
    58621 tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc ttttacttct
    58681 tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg aatacgtctt
    58741 gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc aacgttcgtc
    58801 atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat ctcaaaatag
    58861 tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga tatgattttc
    58921 agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt
    58981 tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt cacgatctcc
    59041 gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca gtaacgcacc
    59101 aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat agacagaaaa
    59161 atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg tattgatccc
    59221 ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa tgatatcata
    59281 cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta cacggttcaa
    59341 gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt ccatgattcc
    59401 acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca gctgaacgtt
    59461 tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg tactttttcc
    59521 aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt atttatgaag
    59581 aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat aaatcagatg
    59641 agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt
    59701 gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa attctcgcca
    59761 ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct cctgcgttct
    59821 cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt aagagagatt
    59881 gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc ttgacttgcg
    59941 cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat ggaagacatg
    60001 acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca atgggctttt
    60061 gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc acgtgaaaca
    60121 gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt acctgatgaa
    60181 ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt ggcatttgcg
    60241 acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct agcgcaagga
    60301 aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat ctatgaagca
    60361 gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa
    60421 gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg tacactgaca
    60481 attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga agaaacattt
    60541 ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag tgtgaaagaa
    60601 taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca tgtacgggta
    60661 cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt tttcttcaaa
    60721 aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga tacagaactt
    60781 gtatagaagg ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac atactttcct
    60841 tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta gatagaaggg
    60901 agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg tatcagtaac
    60961 aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga caagaaaaaa
    61021 agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt caaatgtgat
    61081 caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt tcttcttctc
    61141 caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga tcatgttatg
    61201 caattctcgt catagccaag aaaatgagat caagtattta caagagcttt cccaccgctc
    61261 agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg cattagacag
    61321 cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc gtcgggataa
    61381 cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc tgtttattga
    61441 aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc aattagaaga
    61501 acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta atgggaaatt
    61561 tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa aattactcaa
    61621 acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg gatgctatca
    61681 ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag gattcgatgg
    61741 attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc caagttttga
    61801 tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc aaagaaaagt
    61861 tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag tattgacaaa
    61921 gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt agtgcttttt
    61981 ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata atgtataata
    62041 ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga aaaaaagagg
    62101 gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta tacgaatgga
    62161 aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca agacatgaca
    62221 aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt catcgatttg
    62281 agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg tgacacgacc
    62341 acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa aatgtctgga
    62401 cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc cggatttcag
    62461 atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt agcggtaatt
    62521 ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg tgacgtcaca
    62581 gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa aatcgcggca
    62641 ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat gaagaacatc
    62701 gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc taaccgagaa
    62761 acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg aaatagtcta
    62821 gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct agtagaaatg
    62881 tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac aaccgatgaa
    62941 gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa attcaaagaa
    63001 atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg aattttgact
    63061 gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca
    63121 aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat
    63181 attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt
    63241 gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat tgaattatta
    63301 tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca tgacatcatc
    63361 acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa agaaatggaa
    63421 ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga agctgtgatg
    63481 caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa tccagcatgg
    63541 gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac agtaatcggg
    63601 tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga tgccatcaat
    63661 aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc aaaggatgat
    63721 caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc
    63781 aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc ttgcgaaata
    63841 gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac aggcggagca
    63901 aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct
    63961 tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg tgctacaaga
    64021 atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg ctattaattt
    64081 tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca gctattgaag
    64141 cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt ctcgtaacgg
    64201 aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt
    64261 gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc
    64321 tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt cgacaagtaa
    64381 tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat ggtgtgacga
    64441 aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat ctataactca
    64501 tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag gctttacaga
    64561 aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag ggttattttt
    64621 aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct catcagacac
    64681 aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat tcaaccagtc
    64741 agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag gaaatgatgg
    64801 cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg accaagcatt
    64861 gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag cgatccaaga
    64921 acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca tcgatggatt
    64981 agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg caggtgttgc
    65041 agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt
    65101 aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga aagaattagg
    65161 aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag ataaaggacg
    65221 ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg cttcaggcgg
    65281 tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt
    65341 ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat taaacggcga
    65401 aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag tagaagattt
    65461 ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt atggattgaa
    65521 agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa aagcagttga
    65581 tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa
    65641 ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg tttgattaga
    65701 acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg aacaactccc
    65761 tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt
    65821 gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag
    65881 aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac atcaatttac
    65941 aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc aaatctacat
    66001 tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg atgcgtggga
    66061 aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg atggtccatc
    66121 agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg ggaagcgagc
    66181 ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa aaagtttctg
    66241 aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca gttggaatgg
    66301 aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg atttttgatg
    66361 agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat
    66421 tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa atcaaagcag
    66481 tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc aacgttaaag
    66541 acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca ttcaaaacga
    66601 tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag
    66661 aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac
    66721 ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct ttgactggtt
    66781 tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact aataaaaaac
    66841 cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat aaatacgggt
    66901 tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat caaaaacctt
    66961 acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga gaattgatcg
    67021 aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa
    67081 atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg
    67141 caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg
    67201 aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa gaaattttaa
    67261 atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc gttgatccga
    67321 aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta gaagaagcac
    67381 gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa attccgtaat
    67441 ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat attgatggct
    67501 gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc ccttggttcg
    67561 caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac agcattaggt
    67621 ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca agcactttgc
    67681 gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa agttgttttg
    67741 cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc tgttccaggt
    67801 ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat gaactatatc
    67861 ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt
    67921 ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg gttaacacaa
    67981 gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc gcttattttc
    68041 gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt tggattgaat
    68101 ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc
    68161 atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac gtatcaaaac
    68221 ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt ttccttgctt
    68281 ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat tttgaagatc
    68341 ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt
    68401 gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc
    68461 ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa
    68521 aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg atccttactt
    68581 ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact ttttctgaac
    68641 gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct tttagttcca
    68701 tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg atcgctattt
    68761 tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca acagtcagtt
    68821 tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct gctttaggtg
    68881 ttttcttgat caaagcatgg tatggtaaag gtcaaactga taacatcacg cagaatctgg
    68941 ggtatttttc tttcccagga ttagcagata ttccggtcat tggaccaatt ttctttaaaa
    69001 atacattctt acctgcttat tgtgctattt tgattgctgt cctagcttgg gtgatcatct
    69061 tcaagacgaa attcggcttg cgcctccgtt cagttggtga aaatcctcaa gctgccgaca
    69121 cattaggtat caatgtatat gctatgcgtt attcaggcgt actgatttct ggcttgttag
    69181 gcggtatggg cggtgctgtt tttgctcagt cgatttccgg taacttttca gctggtacga
    69241 tcgttggaca agggttcatc tccatggctg ctatgatctt tggtaagtgg aatcctttag
    69301 gggcaatggg tgcctcattg ttctttggtt ttgctcaaag cttgagtatc attggcgcac
    69361 aattaccagt tatttcttcg attccgccag tattgctgca aattgctcca tatttgctga
    69421 caatcattgt gctggttgtt ttcttaggga aagcttctgg tccaaaagca aacgggaaga
    69481 actatatcaa atcaaaataa tcttttagcg aataaaaagg gtgggttacg ggaagtgttt
    69541 ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag gaataatcat
    69601 gtttaaacgc gtacatctga tcgtcatgga ttcagtagga attggagaag caccagatgc
    69661 cgataagttc ggtgatgttg gttctgatac attaggacac attgcgaaag aagcaggatt
    69721 gaacgttcca catttagagc agcttggttt aggaacgatc cgtcctcttg aaggtgtgga
    69781 atcagtgaaa gatcatcaag gctatgcgac gaaattagaa gaagtttctg tagggaaaga
    69841 tacgatgaca ggccactggg aaatcatggg attgaatatc aaaactccat ttcgtgtgtt
    69901 tcctaatgga ttccctgatg atctgatcaa acaaatcgaa gaattttctg gccgtaaagt
    69961 cgtatgtaac aaaccttata gcggaacagc tgttattgac gattacggtg aacaccaaat
    70021 gaaaacaggc gatttaattg tatatacttc tgccgatcct gtccttcaga tcgcggcaca
    70081 tgaagaaatc attcctttag aagaattata tcggatttgc caattcgttc gtgatataac
    70141 aaaagatgat ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg
    70201 agacttcaca cgaacaagca atcgccatga ttatgcgttg aatccattcg gacacacagt
    70261 ccttgattct ttgaaagaag caggaaagga cgtgattgct gttggaaaga tcaacgatat
    70321 tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga
    70381 ccagctgctg aaagttatga aaaacgattt ttcaggaatc agctttacca atttagtaga
    70441 ttttgatgca ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga
    70501 atttgactta cgaattccag aattgcttga agcgatggaa gaagacgact tgctaatgat
    70561 tacagccgat cacggaaacg atccgacgtt tactggtacc gatcatacgc gagaatatgt
    70621 accgttattg gtttacagta aaaaaatgaa agaacaaggg gcattgcctc aaggatatta
    70681 ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga
    70741 aagcttcttg aaattattaa aataatcttt ttaaatagga gaaagcaaat gaccaagtta
    70801 agcgagatgt tacaagaaac gacccagttt atccttgata aaggagtaaa agaaattgat
    70861 tttggtttga ttctaggttc aggattaggt gaattagcag atgaaatcga agatgctgta
    70921 atgatttctt atgatgagat cccgttcttt cctgtttcga cagtagttgg gcatgcagga
    70981 aagctagttt atgggtctct ggcaggcaag aaagtgttag ccatgcaagg gagattccac
    71041 tactatgaag gtcattccat gcaaacagtg acctatccag tacgtgtgat ggccggacta
    71101 ggggcccatt cagtcattgt gactaatgca tgtggtggtg tcaacgaatc atttgtacca
    71161 ggagatttga tgctgattac cgatcatatc aattttactg gtgaaaatcc tttgatcggt
    71221 ccgaatgaag atgcaatggg ccctagattt ccagatatga gtcaagctta tacgcctgac
    71281 tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt tggatcttaa agaaggcgta
    71341 tatatgggct attctggacc aacttatgaa acgcctgcgg agatccgaat gtctcggaca
    71401 ataggagccg atgcagtagg gatgtcgact gttccagaag tcatcgtagc agctcacagc
    71461 ggattaaaag tgttaggaat ctcttgtatt accaacttag cagcaggtat gcaagcgaat
    71521 ctgagccatg aagaagttgt agaaacaact cagagagtca aacaatcttt taaagcattg
    71581 gttaaagaaa cattgacatt attgtaatag gaggaacaat aaaatgagtg tgcatattga
    71641 agcgaaagaa ggcgagatcg ccgataagat tttacttccg ggagatcctt tgcgagctaa
    71701 atatattgcg gagacttttt tggaagatcc agtttgttac aatcaggtac gcggtatgtt
    71761 aggctttacc ggcaaatata aaggagaacg tatttctgtt caaggaacag gaatgggaat
    71821 gccatcagcc accatctatg cccatgaatt gatccagtcg tatggtgtaa agaaattgat
    71881 tcgagtaggt acatgcggtg cattgtcaaa agacgtccat gttcgtgatt tagtactagc
    71941 acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc
    72001 tccaatcagt gatttcaatc tattactgaa agcatatgag atagctaaag aaaagggcta
    72061 tacggttcat gtaggaaatg ttctttcgga agattctttc tacaaagatg atttgacaga
    72121 gacgttccaa ttagctgaac ttggcgtttt aggagtggaa atggaagcag cggctcttta
    72181 ttacttaggc gcgaaatacc atgtgcagac attaagtttg atgaccgtta gcgaccattt
    72241 gatcaccggt gaagaaacaa cagctgcaga acgtcaatct acgttcaatg aaatgatcga
    72301 agtaggattg gaaacagcga tagcagaata agaaggaaat gaaaaagctg ccctgcatta
    72361 agtaataaga acatccaagc aaaaatagct cctcatgttt ttgacttggc tggacttatt
    72421 attgcaaatg taagcttttg aacaccgttt accaagaggt tgtgaatctg ccgtttaact
    72481 ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt
    72541 gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg
    72601 tgtcacagtc ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt
    72661 catcatttaa ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt
    72721 cgtcagagat accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta
    72781 ctaaaacagt tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag
    72841 cgcgttctaa tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg
    72901 cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag
    72961 tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt
    73021 ttttgttcaa accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt
    73081 gtggtaccca aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag
    73141 ttaaaacaga agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc
    73201 cttcttttgc ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt
    73261 ggttcaatgc attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta
    73321 gctctccttt taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat
    73381 tttttggaag agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga
    73441 atataaaaaa acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt
    73501 gctcgatttc tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc
    73561 attttgaaga aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt
    73621 tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat
    73681 ctaatccaac taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca
    73741 ttaattggaa atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca
    73801 agtgcagccc gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc
    73861 ctaatcgatg aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg
    73921 actccaaatg aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt
    73981 cttgacaacc aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg
    74041 ttgtgcgtgc ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact
    74101 ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt
    74161 tccgctgtct ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt
    74221 caagaatctc aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa
    74281 gcccatgttc tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt
    74341 cgatgacttc tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc
    74401 cgttttcgtc tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct
    74461 gttgggagcc ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta
    74521 atttagaaga atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga
    74581 aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac
    74641 cgtctattgt caaatcgact acggggactt catcaatact ctttaacggt attcctaatt
    74701 tttccgcttg ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt
    74761 cacgcatacg gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac
    74821 ctacgatcat cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat
    74881 tcatctttgc gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt
    74941 gatttacggt aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt
    75001 gcatctcttg ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca
    75061 ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca
    75121 ttgcatttat tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat
    75181 cgtgggaagg aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa
    75241 atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa
    75301 aactcaaact aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat
    75361 gacacctaaa aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt
    75421 aattgaagtt actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt
    75481 attattacgc ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg
    75541 tgctttagat actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa
    75601 aaaacctaaa gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag
    75661 taattactat gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt
    75721 gcaaaacgtg atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc
    75781 aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt
    75841 gacttgatca aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa
    75901 aacgttcaac ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta
    75961 ccagttgaag ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac
    76021 gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct
    76081 gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc
    76141 aaccgtgcgt ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag
    76201 tcgacctcag atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg
    76261 agaatgacca gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc
    76321 gatgctggta aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa
    76381 attggtgaaa ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt
    76441 ggtattacga tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc
    76501 atcgacacac caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt
    76561 gatggtgctg taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt
    76621 tggcgtcaag caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag
    76681 atcggtgctg acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct
    76741 cacccaatcc aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta
    76801 acaatgaagg ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt
    76861 cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct
    76921 gaaactgatg aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag
    76981 ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt
    77041 tcagcattta agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca
    77101 tcaccgttag atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa
    77161 cgtcctgcag acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca
    77221 ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac
    77281 gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc
    77341 aactctcgtt cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg
    77401 aaagacacaa ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc
    77461 atggaattcc cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa
    77521 gataaaatgg gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa
    77581 acaaacgtgg aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc
    77641 atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta
    77701 tcttaccgtg aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct
    77761 ggtggtaaag gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct
    77821 ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct
    77881 gttgaaactg gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta
    77941 gatatcaaag ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc
    78001 ttccgtgttg ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc
    78061 cttgaaccaa tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg
    78121 ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa
    78181 caagttgttc gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt
    78241 tctgctactc aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca
    78301 aaatcagtac aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa
    78361 accggatagt cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt
    78421 aaaataatta tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag
    78481 acaaataaaa aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt
    78541 gaccgttcta aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact
    78601 acattaacag ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct
    78661 tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct
    78721 cacgttgagt atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg
    78781 gattacgtta aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt
    78841 tctgctgctg acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt
    78901 ggtgttcctt acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta
    78961 ctagaattag ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac
    79021 gatgttcctg tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa
    79081 gaaaaaattc ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac
    79141 aacgacaaac cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact
    79201 gttgctacag gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt
    79261 ggtattgctg aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg
    79321 ttagactacg ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa
    79381 gacatccaac gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc
    79441 tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact
    79501 aactaccgtc cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca
    79561 gaaggaactg aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac
    79621 ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt
    79681 gccggtgtcg ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt
    79741 tattcggacg taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt
    79801 tattgcagga tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag
    79861 agtcagtatt ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct
    79921 tgctttgtga gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac
    79981 agacgtaatc agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc
    80041 tttgccacga acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg
    80101 cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca
    80161 ccgtatttta gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag
    80221 cgtgtctggt ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac
    80281 tcataaatac aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga
    80341 cattgtgaac ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg
    80401 tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac
    80461 caaaggaatc ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt
    80521 aattccagta actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga
    80581 aacggatggc tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa
    80641 caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga
    80701 attcaaaaat gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt
    80761 ccaagcagga gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt
    80821 tatcaaacgc cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg
    80881 tcctgggtca atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg
    80941 ccgtatgggt ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga
    81001 aagaaatgtt atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat
    81061 caaatcagct gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa
    81121 tgtagcatta ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat
    81181 cttcggaatt gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc
    81241 attaagacaa ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa
    81301 accatggcgt caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg
    81361 gcgtggaggt ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa
    81421 aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt
    81481 ggtagcaatc gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct
    81541 tgctaactta tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc
    81601 tgctttatca gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt
    81661 gctagacgtt gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga
    81721 ggaggtgctt gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa
    81781 tcaatgcttg ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa
    81841 actttagtaa aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc
    81901 gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt
    81961 cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct
    82021 gaataagcac taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt
    82081 acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa
    82141 catcaacacc tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca
    82201 acggccgcat cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg
    82261 acttcaaacg taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa
    82321 accgttcagc taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag
    82381 cacctaaagg actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag
    82441 tcggaaatgc tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa
    82501 tgaaacctgg taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg
    82561 gtaaagaagg caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag
    82621 ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg
    82681 gtaaagctgg acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga
    82741 accctaatga tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac
    82801 cagtttctcc ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat
    82861 cagacaaact tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac
    82921 attttgagag gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc
    82981 atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt
    83041 ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg
    83101 gacgtaaaca tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat
    83161 ttgcacctac aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat
    83221 ttgaggggag gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa
    83281 acagttcgca cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt
    83341 gtcgcagatg caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa
    83401 aaagtattga tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac
    83461 ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt
    83521 gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca
    83581 gaaaaataag gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg
    83641 catcatccgc gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca
    83701 cgaagattta agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac
    83761 agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg
    83821 tatggttatt ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt
    83881 aactggtaaa agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa
    83941 attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca
    84001 aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc
    84061 tggtcgtcta aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc
    84121 tcttcatact ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg
    84181 aaaactagga gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac
    84241 tgagaaagga gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc
    84301 cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt
    84361 ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca
    84421 atgactcgtt acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct
    84481 tatactgcca aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg
    84541 gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta
    84601 gctcgtgaag cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa
    84661 cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc
    84721 cgaaatgctt gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt
    84781 agcaacaggt caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg
    84841 catcaaaact gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat
    84901 gactgaagga agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga
    84961 taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat
    85021 gaactattct aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat
    85081 cgtgagaatt atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt
    85141 agtcgaagaa gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa
    85201 atctgaaagg aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa
    85261 ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc
    85321 taacatcggt gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa
    85381 aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga
    85441 cggttcatat attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc
    85501 tcgtggaaca cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa
    85561 gatcgtttca ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga
    85621 aacagttatg tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa
    85681 caaagaaggc gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt
    85741 aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga
    85801 gatggaagcg ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg
    85861 ccgagttggc tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga
    85921 agttttagat aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga
    85981 aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat
    86041 gcaaacacct aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa
    86101 cgctaaaaac ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat
    86161 gattacaaaa gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg
    86221 tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt
    86281 ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg
    86341 aaactatact ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt
    86401 agataaagta cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc
    86461 tcgtgagttg ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta
    86521 cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc
    86581 ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg
    86641 ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag
    86701 ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca
    86761 attgcagatt ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa
    86821 gtacctgcat caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt
    86881 cgtgatgttg aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt
    86941 ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt
    87001 gcttatgtca aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt
    87061 tcgacttctg aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa
    87121 gttatcgctt acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg
    87181 tatcggaaat aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa
    87241 cgttacagtt aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat
    87301 gaatatcgaa ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac
    87361 aattcatgga acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt
    87421 ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact
    87481 tgtattgaac gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt
    87541 tgaagtacca gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga
    87601 attagctgca aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg
    87661 ttatgttggc gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa
    87721 aattgcatag aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa
    87781 tacattttta cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt
    87841 agtacaataa aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac
    87901 gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct
    87961 tgaacgtttt tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg
    88021 taacgctagc aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag
    88081 cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag
    88141 tagtctttga ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg
    88201 ctcgcgaaaa tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga
    88261 tccaaaacat ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt
    88321 taaaggtgga cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca
    88381 cgtagggttt ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga
    88441 agatgcgaag aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt
    88501 aatcggtgct tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt
    88561 tgctgctggt ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc
    88621 taaatcttta ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa
    88681 acaattgaaa cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat
    88741 cggataagga ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc
    88801 ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt
    88861 tcagtggtta agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta
    88921 gtggacgttg aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa
    88981 acctatgaaa cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt
    89041 tggtcgtggt acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa
    89101 agctcgttca ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg
    89161 tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct
    89221 tgatgtattg aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc
    89281 tggaatcgtg aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa
    89341 gaaattaact gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc
    89401 tggtggatca atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa
    89461 gacattagat cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca
    89521 catattactg taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt
    89581 ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg
    89641 ggggtttcac catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg
    89701 cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg
    89761 actcgatatc tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc
    89821 aatcaatacg ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc
    89881 ggtttgattc taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa
    89941 aaaggtatcg gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct
    90001 gaaagtatca gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg
    90061 aaatctgcta tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta
    90121 acatttttcc aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc
    90181 gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc
    90241 tttgctagtt cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc
    90301 ggttctgaaa actggtacaa agtgatgatg gagatcttta actataatac agcacctggt
    90361 gctacgattt atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta
    90421 aaccctgaga aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc
    90481 cctggtaagg ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt
    90541 gcagtcttcc ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt
    90601 ccacaatcaa tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa
    90661 actgcgaaac agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa
    90721 gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac
    90781 aatgaacctc attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat
    90841 cattgatgct tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca
    90901 aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc
    90961 tgatgaagta acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg
    91021 cttcttatta gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct
    91081 aaaagatttg aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt
    91141 ggtcgaacgt ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat
    91201 tttcaaccct acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca
    91261 aagagaagat gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag
    91321 tgaaccaatc ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg
    91381 tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc
    91441 gtagccgact tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg
    91501 tcagtccgac ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac
    91561 tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg
    91621 tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa
    91681 tgtttaaagt tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc
    91741 gtatgcatta catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg
    91801 atcttaaccg tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg
    91861 aggtattatc atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat
    91921 tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca
    91981 aggataattg gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat
    92041 aaacgtgtag tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag
    92101 atcttagctg atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa
    92161 acagatgcta tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa
    92221 gtgaacttga acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc
    92281 cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca
    92341 gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg
    92401 gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca
    92461 catatccatt ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca
    92521 ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt
    92581 gccgctcaaa tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact
    92641 gttgaagtaa cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa
    92701 gctacaggtt tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc
    92761 cgccctccaa aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac
    92821 gtcattgaac aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga
    92881 aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc
    92941 tcttgaaaga gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc
    93001 tttgccaggt gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac
    93061 catcaaaggt gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct
    93121 gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt
    93181 aactgctggc gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat
    93241 ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg
    93301 ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga
    93361 ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca
    93421 ccgtgaagat tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt
    93481 ggaagcaatg agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt
    93541 aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa
    93601 aatgttggaa atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa
    93661 acgtgcagga atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa
    93721 agtccgtaac ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg
    93781 tttaggttta cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta
    93841 tcgtaaacta ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga
    93901 tttattaatc aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac
    93961 tgcagaaaaa atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc
    94021 ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga
    94081 atcagcttta caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg
    94141 atacacacgc attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt
    94201 agaacttgtc taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta
    94261 tgatgttgga agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct
    94321 tggggagtaa gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag
    94381 atatgtttga aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt
    94441 tttgatagtg ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg
    94501 aattagaaaa gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg
    94561 tctcctttac aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa
    94621 aatcgacact tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag
    94681 tgggaaatca aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg
    94741 tttttcaaaa tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg
    94801 gtcttgaaaa tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg
    94861 agaaagttag aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa
    94921 aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg
    94981 aagcaaccag tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga
    95041 tcaaagaaga aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta
    95101 atgccaatcg gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag
    95161 aaattttttc tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa
    95221 aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag
    95281 aaaggatggt ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc
    95341 gaatacacca tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag
    95401 ttatactgca ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa
    95461 tgcgcttgtt aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga
    95521 tacggataat aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc
    95581 agaagcacag ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt
    95641 tggtgtcagc gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct
    95701 ggatgaaagc tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt
    95761 agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc
    95821 aggacttgat ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga
    95881 acagcagatc actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga
    95941 ttatgtctat gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt
    96001 tcaaaacatt gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc
    96061 agaagaatta atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt
    96121 agcagacgct atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg
    96181 gtcgttatat gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag
    96241 ccagttttta ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt
    96301 tggcattttt cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc
    96361 gaggcattcg cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta
    96421 caagtggcgg agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga
    96481 tgaacggcct ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca
    96541 cgcttactac accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga
    96601 aagccgtacg gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg
    96661 tgcctacatt gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg
    96721 attttggtga aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt
    96781 tatttgtcag cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct
    96841 acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga
    96901 tggttatcat tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt
    96961 tcagtgggcc gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag
    97021 tgtatgacaa gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat
    97081 ctgaaaagaa agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg
    97141 aacaaaggag agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat
    97201 tttaatggat tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca
    97261 ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc
    97321 ggagtacatg cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa
    97381 cgaatgcgtt tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa
    97441 attgtttcag aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga
    97501 gtggatatag ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac
    97561 cctcttgatt tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc
    97621 acctcttttt gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct
    97681 aagatggagg taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc
    97741 ttgtataaga tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg
    97801 cctgaagata gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca
    97861 gcacatcctg aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa
    97921 gtggtcagct tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa
    97981 atttcggctt attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa
    98041 agctgccctg catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt
    98101 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
    98161 aaatcagcgt tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    98221 gccgtttgtt gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg
    98281 tgagcctgtc atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt
    98341 tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta
    98401 ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc
    98461 ggaacaggtt tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt
    98521 agcgctgaaa atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa
    98581 tatttacgcc atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc
    98641 gtttttgcag tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa
    98701 aacaatcagg tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact
    98761 tggcagttgg aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt
    98821 ctacactctt agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact
    98881 aagtacttta ttttactctt taggattgat acagtagcat ctatcccttt attaataatc
    98941 ttttattttt ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa
    99001 ttcctttttc tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag
    99061 cttccatatg aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat
    99121 cgtctttccc cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta
    99181 ctatgatttc tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat
    99241 ctcctgtaaa gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac
    99301 ctacataatt caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag
    99361 attcaaaaac tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt
    99421 cattcgcatc attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg
    99481 ctatatcgtc ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga
    99541 taatatcttc gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg
    99601 atggaaaagg acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat
    99661 caattaaaaa ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta
    99721 tattcataaa cgttgtctcc tttttgttga tatgaaatat ttttagtata attcaattcc
    99781 atacaataaa taaaccgaac aaaaaaagtc ttttactatt tttacttaat ttatgaaagg
    99841 ttgtttacca tgttagacca aacagatata aatatcattg aggaactatc aaaaaatagt
    99901 cgaatcacaa tgaaagaact tggagaaaaa gtacatttga ctggacccgc tgtctcggcg
    99961 agagtgacaa aactagagga aagtggagtt attgaaaatt atacagtaaa attaaatatg
   100021 gaaaagctag ggttttcgat tcacgctttt ttaattataa ttacgcaaaa tttaaatcat
   100081 aaaccctatt tatcatttat taaaaatcat gaaaaattct tgattagaaa ttataagatt
   100141 agtggagggg gctgttatct tttggaatgt agatttactt caaaaaaatc aatgaacagc
   100201 tttttggaac agcttaatga atatgcgaat tataaattat ctattgttat agattaataa
   100261 taaaactagc agatggtaaa aatactaata tgcgagacac ac
//
LOCUS       DAZUKT010000002        95282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000002 DAZUKT010000000
VERSION     DAZUKT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.002"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(5..280)
                     /locus_tag="ACG816_000109"
     CDS             complement(5..280)
                     /locus_tag="ACG816_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919032.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            512..1246
                     /locus_tag="ACG816_000110"
     CDS             512..1246
                     /locus_tag="ACG816_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4919033.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1285..2427
                     /locus_tag="ACG816_000111"
     CDS             1285..2427
                     /locus_tag="ACG816_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4919034.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2522..3913)
                     /gene="sufB"
                     /locus_tag="ACG816_000112"
     CDS             complement(2522..3913)
                     /gene="sufB"
                     /locus_tag="ACG816_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4919035.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(3918..4388)
                     /gene="sufU"
                     /locus_tag="ACG816_000113"
     CDS             complement(3918..4388)
                     /gene="sufU"
                     /locus_tag="ACG816_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4919036.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(4375..5610)
                     /locus_tag="ACG816_000114"
     CDS             complement(4375..5610)
                     /locus_tag="ACG816_000114"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4919037.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(5607..6893)
                     /gene="sufD"
                     /locus_tag="ACG816_000115"
     CDS             complement(5607..6893)
                     /gene="sufD"
                     /locus_tag="ACG816_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4919038.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(6906..7679)
                     /gene="sufC"
                     /locus_tag="ACG816_000116"
     CDS             complement(6906..7679)
                     /gene="sufC"
                     /locus_tag="ACG816_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4919039.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(7876..8724)
                     /locus_tag="ACG816_000117"
     CDS             complement(7876..8724)
                     /locus_tag="ACG816_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4919040.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(8748..9434)
                     /locus_tag="ACG816_000118"
     CDS             complement(8748..9434)
                     /locus_tag="ACG816_000118"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4919041.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(9427..10461)
                     /locus_tag="ACG816_000119"
     CDS             complement(9427..10461)
                     /locus_tag="ACG816_000119"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4919042.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(10524..10780)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10856..11194)
                     /locus_tag="ACG816_000120"
     CDS             complement(10856..11194)
                     /locus_tag="ACG816_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4919043.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(11196..11552)
                     /locus_tag="ACG816_000121"
     CDS             complement(11196..11552)
                     /locus_tag="ACG816_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4919044.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(11558..12745)
                     /locus_tag="ACG816_000122"
     CDS             complement(11558..12745)
                     /locus_tag="ACG816_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4919045.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(12936..13781)
                     /locus_tag="ACG816_000123"
     CDS             complement(12936..13781)
                     /locus_tag="ACG816_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4919046.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            14015..14980
                     /locus_tag="ACG816_000124"
     CDS             14015..14980
                     /locus_tag="ACG816_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4919047.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            15357..16400
                     /locus_tag="ACG816_000125"
     CDS             15357..16400
                     /locus_tag="ACG816_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4919048.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16462..16926)
                     /locus_tag="ACG816_000126"
     CDS             complement(16462..16926)
                     /locus_tag="ACG816_000126"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4919049.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            17172..18119
                     /locus_tag="ACG816_000127"
     CDS             17172..18119
                     /locus_tag="ACG816_000127"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4919050.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(18330..20402)
                     /locus_tag="ACG816_000128"
     CDS             complement(18330..20402)
                     /locus_tag="ACG816_000128"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4919051.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(20335..20712)
                     /locus_tag="ACG816_000129"
     CDS             complement(20335..20712)
                     /locus_tag="ACG816_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4919052.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(20841..21401)
                     /locus_tag="ACG816_000130"
     CDS             complement(20841..21401)
                     /locus_tag="ACG816_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4919053.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            21551..22843
                     /locus_tag="ACG816_000131"
     CDS             21551..22843
                     /locus_tag="ACG816_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4919054.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(22903..24021)
                     /locus_tag="ACG816_000132"
     CDS             complement(22903..24021)
                     /locus_tag="ACG816_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4919055.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(24035..25456)
                     /gene="arcD"
                     /locus_tag="ACG816_000133"
     CDS             complement(24035..25456)
                     /gene="arcD"
                     /locus_tag="ACG816_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4919056.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(25702..26823)
                     /gene="mnmA"
                     /locus_tag="ACG816_000134"
     CDS             complement(25702..26823)
                     /gene="mnmA"
                     /locus_tag="ACG816_000134"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4919057.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            27227..27826
                     /locus_tag="ACG816_000135"
     CDS             27227..27826
                     /locus_tag="ACG816_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4919058.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(27964..29250)
                     /gene="efmA"
                     /locus_tag="ACG816_000136"
     CDS             complement(27964..29250)
                     /gene="efmA"
                     /locus_tag="ACG816_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4919059.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(29521..29865)
                     /locus_tag="ACG816_000137"
     CDS             complement(29521..29865)
                     /locus_tag="ACG816_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4919060.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(30002..31147)
                     /locus_tag="ACG816_000138"
     CDS             complement(30002..31147)
                     /locus_tag="ACG816_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4919061.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(31327..32301)
                     /locus_tag="ACG816_000139"
     CDS             complement(31327..32301)
                     /locus_tag="ACG816_000139"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4919062.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(32381..33256)
                     /locus_tag="ACG816_000140"
     CDS             complement(32381..33256)
                     /locus_tag="ACG816_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4919063.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            33386..35293
                     /locus_tag="ACG816_000141"
     CDS             33386..35293
                     /locus_tag="ACG816_000141"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS4919064.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(35834..39112)
                     /locus_tag="ACG816_000142"
     CDS             complement(35834..39112)
                     /locus_tag="ACG816_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4919065.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(39383..39928)
                     /locus_tag="ACG816_000143"
     CDS             complement(39383..39928)
                     /locus_tag="ACG816_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4919066.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(39940..40632)
                     /locus_tag="ACG816_000144"
     CDS             complement(39940..40632)
                     /locus_tag="ACG816_000144"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4919067.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(40651..40935)
                     /gene="macP"
                     /locus_tag="ACG816_000145"
     CDS             complement(40651..40935)
                     /gene="macP"
                     /locus_tag="ACG816_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4919068.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(40948..41511)
                     /locus_tag="ACG816_000146"
     CDS             complement(40948..41511)
                     /locus_tag="ACG816_000146"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4919069.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            41739..42401
                     /locus_tag="ACG816_000147"
     CDS             41739..42401
                     /locus_tag="ACG816_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4919070.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            42525..43721
                     /locus_tag="ACG816_000148"
     CDS             42525..43721
                     /locus_tag="ACG816_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4919071.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(43822..45198)
                     /locus_tag="ACG816_000149"
     CDS             complement(43822..45198)
                     /locus_tag="ACG816_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4919072.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(45235..46101)
                     /locus_tag="ACG816_000150"
     CDS             complement(45235..46101)
                     /locus_tag="ACG816_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4919073.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(46190..47215)
                     /locus_tag="ACG816_000151"
     CDS             complement(46190..47215)
                     /locus_tag="ACG816_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4919074.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(47233..47907)
                     /locus_tag="ACG816_000152"
     CDS             complement(47233..47907)
                     /locus_tag="ACG816_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4919075.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(48091..48984)
                     /locus_tag="ACG816_000153"
     CDS             complement(48091..48984)
                     /locus_tag="ACG816_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4919076.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(49058..50755)
                     /locus_tag="ACG816_000154"
     CDS             complement(49058..50755)
                     /locus_tag="ACG816_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4919077.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(50748..52061)
                     /locus_tag="ACG816_000155"
     CDS             complement(50748..52061)
                     /locus_tag="ACG816_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4919078.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(52061..56002)
                     /locus_tag="ACG816_000156"
     CDS             complement(52061..56002)
                     /locus_tag="ACG816_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4919079.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(56111..56569)
                     /locus_tag="ACG816_000157"
     CDS             complement(56111..56569)
                     /locus_tag="ACG816_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4919080.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(56867..57385)
                     /locus_tag="ACG816_000158"
     CDS             complement(56867..57385)
                     /locus_tag="ACG816_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4919081.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(57387..58004)
                     /locus_tag="ACG816_000159"
     CDS             complement(57387..58004)
                     /locus_tag="ACG816_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4919082.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(58001..58855)
                     /locus_tag="ACG816_000160"
     CDS             complement(58001..58855)
                     /locus_tag="ACG816_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4919083.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(58852..59763)
                     /locus_tag="ACG816_000161"
     CDS             complement(58852..59763)
                     /locus_tag="ACG816_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4919084.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(59804..61138)
                     /locus_tag="ACG816_000162"
     CDS             complement(59804..61138)
                     /locus_tag="ACG816_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4919085.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            61346..62176
                     /locus_tag="ACG816_000163"
     CDS             61346..62176
                     /locus_tag="ACG816_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS4919086.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(62420..62669)
                     /locus_tag="ACG816_000164"
                     /pseudo
     CDS             complement(62420..62669)
                     /locus_tag="ACG816_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(62860..63054)
                     /locus_tag="ACG816_000165"
     CDS             complement(62860..63054)
                     /locus_tag="ACG816_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919087.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(63559..63744)
                     /locus_tag="ACG816_000166"
     CDS             complement(63559..63744)
                     /locus_tag="ACG816_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919088.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(64594..64935)
                     /locus_tag="ACG816_000167"
     CDS             complement(64594..64935)
                     /locus_tag="ACG816_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4919089.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(64935..65165)
                     /locus_tag="ACG816_000168"
     CDS             complement(64935..65165)
                     /locus_tag="ACG816_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4919090.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            65452..65790
                     /locus_tag="ACG816_000169"
     CDS             65452..65790
                     /locus_tag="ACG816_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919091.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(65995..66557)
                     /locus_tag="ACG816_000170"
                     /pseudo
     CDS             complement(65995..66557)
                     /locus_tag="ACG816_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(67289..67870)
                     /locus_tag="ACG816_000171"
     CDS             complement(67289..67870)
                     /locus_tag="ACG816_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4919092.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(68053..68679)
                     /locus_tag="ACG816_000172"
     CDS             complement(68053..68679)
                     /locus_tag="ACG816_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4919093.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(68701..70032)
                     /locus_tag="ACG816_000173"
     CDS             complement(68701..70032)
                     /locus_tag="ACG816_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4919094.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(70187..70588)
                     /locus_tag="ACG816_000174"
     CDS             complement(70187..70588)
                     /locus_tag="ACG816_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4919095.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(70674..70880)
                     /locus_tag="ACG816_000175"
     CDS             complement(70674..70880)
                     /locus_tag="ACG816_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919096.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(70975..72477)
                     /locus_tag="ACG816_000176"
     CDS             complement(70975..72477)
                     /locus_tag="ACG816_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4919097.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(72495..72950)
                     /gene="argR"
                     /locus_tag="ACG816_000177"
     CDS             complement(72495..72950)
                     /gene="argR"
                     /locus_tag="ACG816_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4919098.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(73048..73665)
                     /locus_tag="ACG816_000178"
     CDS             complement(73048..73665)
                     /locus_tag="ACG816_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4919099.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            73886..75259
                     /locus_tag="ACG816_000179"
     CDS             73886..75259
                     /locus_tag="ACG816_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4919100.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            75334..76446
                     /locus_tag="ACG816_000180"
     CDS             75334..76446
                     /locus_tag="ACG816_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4919101.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(76518..78155)
                     /locus_tag="ACG816_000181"
     CDS             complement(76518..78155)
                     /locus_tag="ACG816_000181"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4919102.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(78323..78931)
                     /locus_tag="ACG816_000182"
     CDS             complement(78323..78931)
                     /locus_tag="ACG816_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4919103.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(79315..80466)
                     /gene="queG"
                     /locus_tag="ACG816_000183"
     CDS             complement(79315..80466)
                     /gene="queG"
                     /locus_tag="ACG816_000183"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4919104.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(80485..81237)
                     /locus_tag="ACG816_000184"
     CDS             complement(80485..81237)
                     /locus_tag="ACG816_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919105.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(81378..82328)
                     /locus_tag="ACG816_000185"
     CDS             complement(81378..82328)
                     /locus_tag="ACG816_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4919106.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(82329..83168)
                     /locus_tag="ACG816_000186"
     CDS             complement(82329..83168)
                     /locus_tag="ACG816_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4919107.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(83165..83911)
                     /locus_tag="ACG816_000187"
     CDS             complement(83165..83911)
                     /locus_tag="ACG816_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4919108.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            84149..85519
                     /locus_tag="ACG816_000188"
     CDS             84149..85519
                     /locus_tag="ACG816_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4919109.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(85574..86470)
                     /locus_tag="ACG816_000189"
     CDS             complement(85574..86470)
                     /locus_tag="ACG816_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4919110.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(86584..86754)
                     /locus_tag="ACG816_000190"
     CDS             complement(86584..86754)
                     /locus_tag="ACG816_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919111.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(86751..87428)
                     /locus_tag="ACG816_000191"
     CDS             complement(86751..87428)
                     /locus_tag="ACG816_000191"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4919112.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(87519..88424)
                     /locus_tag="ACG816_000192"
     CDS             complement(87519..88424)
                     /locus_tag="ACG816_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4919113.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            88599..90023
                     /locus_tag="ACG816_000193"
     CDS             88599..90023
                     /locus_tag="ACG816_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4919114.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            90069..91100
                     /locus_tag="ACG816_000194"
     CDS             90069..91100
                     /locus_tag="ACG816_000194"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4919115.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            91107..92294
                     /locus_tag="ACG816_000195"
     CDS             91107..92294
                     /locus_tag="ACG816_000195"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4919116.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            92450..93832
                     /locus_tag="ACG816_000196"
     CDS             92450..93832
                     /locus_tag="ACG816_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4919117.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            93869..94189
                     /locus_tag="ACG816_000197"
     CDS             93869..94189
                     /locus_tag="ACG816_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919118.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(94389..95057)
                     /locus_tag="ACG816_000198"
     CDS             complement(94389..95057)
                     /locus_tag="ACG816_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4919119.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        27177 a        19976 c        17122 g        31007 t
ORIGIN      
        1 ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga
       61 ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta
      121 ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga gtgactccag
      181 aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa
      241 cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga
      301 ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt
      361 tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata
      421 cctcgataaa aaagaggtgt caatatcttt gtaaaattta tttacaacac ccctttttcg
      481 aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt
      541 agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac
      601 acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa aaactatcta
      661 cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt
      721 gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatata ttttgggaat
      781 tattctttat agagatagca ttgtactaga tggtataaca tttccagata aaaacctgtt
      841 ccagcatctt cgtcaacagt tcccaccagt acactctaat ttagatacat ggctggatag
      901 atattccaaa ttagaaccag agatgcaaga atttattgct aacaagactt gtaataaagt
      961 taaaaatgaa aaaattagtc ttttcaatat cttaaaaata gaagaattat tcatcaatgc
     1021 tattgtagaa gaatttgaca ataacattct tttcctcact agcagtatag aagaactact
     1081 agaaagaatg gtagatgaat ggctacctat tcaattgaaa gacatgtctt atcccgaaga
     1141 agctgtgaga gaaattactg ataatataaa caagctagaa caaacgattt catcaattgg
     1201 gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc atctagtggt aagtactcct
     1261 tgtaaactaa gaggagatga tttcatggca aaaatatcaa agtacaaaaa gaaaaacggt
     1321 caagtggcgt ggatgtataa aggacacatt gccaccgatc cacgaactgg tgaaaaaata
     1381 aatactacga gaagaggttt tagtagcaaa caagaagctc aaaaagatta tgacgagtac
     1441 agacatcaaa tattatacgg tgttaaaaag aaagcacctg atatgacatt tgaagacctg
     1501 tataatgaat ggataactca tcagcgtacc agtgttaaag cttcaacaat cgcaatctca
     1561 gtaagatacg caaataatca aatacttcca gcatttggta agcttaaact atcgaatatc
     1621 tctgtcccat attgtcaaaa agtggtagat gaatggcaca gtaaatatga aagttacgat
     1681 tatatgagaa aacagactgc acaaatttta cgctacgggg ttgcaatgca atatattgac
     1741 aataatccta tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa
     1801 ttttactcaa aagaggagct taataattta ctggatgctt ttaaagattt tggtaacatg
     1861 aaacaatacg ccttcttcag attattggca tacacaggta tgagaaagag tgaagtactc
     1921 gctttacagt ggaaagacat agacacgttt aataaagaac tacatgtcaa taaaactctt
     1981 gctgtagatg aatttggtaa agttataatt caatctccga aaacaagagc ttctagacga
     2041 gttatctctt tagatactga gactctttct atcctcaaca actggaaatt acagcaaaaa
     2101 gaagagtatt taaaacttgg atacaatact agctctaaag agcaacatgt ttttacaaca
     2161 gttaaaaaca ctctatacat tccaaacact gttaatgatt ggttaagata tattttgaaa
     2221 aaatataact tgcctcgtat tacacctcat gggtttagac atacacatgc tagtttatta
     2281 ttagaagcag gagaatctgt caaggtagtt caacaacgat tagggcatga aaatagtaaa
     2341 gtgacattag acatctatgc acatattaca aataatgcac ctaagaaaac aggtcaagac
     2401 tttgctgata tgatggctca tcagtaaaag agctggtcaa aatctggtca tttctagtat
     2461 acaggcaaaa caaaaaagct agaaccctta tatatcaagg gttctagctc ctataacagg
     2521 tttacccaac actgccttcc atctcataac tgatcaatcg attgagttcg actgcatatt
     2581 ccattggaag ttctttggtg aatggttcaa caaagcccat gacgatcatt tcagttgctt
     2641 cagattcggt caggcctcgg ctcatcagat agtagagttg ttcttctgag atttttgaga
     2701 ctttggcttc gtgttctaaa gagacttggc tgttgtggat ctcattgaat gggatcgtat
     2761 cggatttcga ccattcgtcc atgatgatcg tgtcacattc gatatgagag atcgaacccg
     2821 cactgttttt ggcaaaggtt acttgtcctc gatagtttac ttctccgccg tcttttgaaa
     2881 ttgatttaga gacgatagag ctagatgtat ttggtgcatt gtggatcatt tttgcacccg
     2941 tatcttggat ttggtttgct cctgcaaagg cgatggagag catggtgcct cgtgcacctt
     3001 ttccatctaa gtaaacactt gggtatttca tcgttgtttt tgcacctagg tttccgtcga
     3061 tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt ggttactaag ttgtacacat
     3121 tgtctgacca gttttggatc gttgtgtaac ggcaatacgc atcttttctt gtaaagattt
     3181 ctacgatggc ggcatgcagg ctgttacttg aataagtagg tgccgtacaa ccttcgacat
     3241 agtggatgct tgctccttcg tcaacaatga tcagcgtacg ttcgaattgc cccgtgtttt
     3301 ccgcattgat ccggaagtac gtttgtaatg gaacatcgac tttgacgcct tttggcacgt
     3361 aaataaaggt cccacctgac catacggctg aattcagcgc tgctaatttg ttgtcggttg
     3421 gcggtacaag ttttgaaaag tactctttga ataattcagg gtattctttc aaagcagagt
     3481 cagtgtcagt aaagacgatt cctaactttt cgaattcatc tttcatatta tggtacacga
     3541 cttctgattc atattgtgcg gaagcaccgg ctagataagc tcgttcagct tctggtatac
     3601 caatcttttc aaatgtatct ttgatttttt ctggtacgtc ttcccagtca cgagcgggtt
     3661 tgtcacttgg tttttggtaa tatttgatgg catcgaaatc gatgtctgaa aggtcggggc
     3721 cccatgtttg cattggcatt ttgttaaatg tttctagaga tttcagacgg aattcaagca
     3781 tccattctgg ttcgcctttg attcttgaca tctcacggat cacttcttct gtcaagcctt
     3841 ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc gaatttatat tcttctaatt
     3901 ctggtacgcc catgttctca ctcctcttcc ttgtggtaat gatggaaagc tgcggtacca
     3961 tcgttttcca ttgcttttcc tagtgctttc caggcaagtg tcgcacattt gatccgagcg
     4021 ggaaatttag ctacaccacc aagcatagca gcatcgccta attggtcggg atctttgact
     4081 tcttttcctt ggacaagctg tagaaagtct acagttaaat gttctgcttc ttctattgat
     4141 tttccgatga cggcgtctgt catcatcgag gcacttgccg tactgatgga gcagccactg
     4201 ccgtcaaagg cgatattttt gatgattccg tcttttacat cgacttgcaa ttcgatcaca
     4261 tctccacatg tggggttgtt caattcgatc ttgcttgtcg agtcagtcaa gcttccacga
     4321 tgatgcggat gggaagaatg atccaagatc acttgacggt ataaattgtc taatttagat
     4381 agtcccatgt tggaaaaact cctttgttgc catgattgct tcaactaaac ggtcggcgtc
     4441 ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc gctgatactt caaggtactt
     4501 caatagcggt tgcgcacaat gatgacctgc tctgacggct accccttcca tgtctaaagc
     4561 tgtcgcgaca tcatggggat gtaacccatc caaattgaat gcaacgactc ctgtccgaaa
     4621 attcgggtct tgtggaccat aaacagtcaa tccatcaatt gcttgtaatt ttggcaacac
     4681 ataagcggct agttcttctt catatcgatg gatattgtcc ataccgagtt tttgcaaata
     4741 atcgattgca tggcctaatg cgatagcgcc ggcgatattt ggtgtccctg cttcgaattt
     4801 ccaaggtagt tctttccatg tactgtcgta taattcgaca aaatcgatca tttcgccacc
     4861 aaattcaaca ggttccattt cttctaacca ttttcgttta ccatataaaa caccaatacc
     4921 tgttggtccg cacattttgt gtccgctgaa agcaaagaaa tcacagtcta gtgcttggac
     4981 atcaatggcc atgtgcgggg cagattgagc accatctact acgatgaccc cgccgttttc
     5041 atggacaagt tcagctaatt cttgaacagg attgaagaca cctaagacat tggatacatg
     5101 agcaatggag acgattttgg ttttgtccgt aatctttttc gctgcatcct ccatatccag
     5161 gaagccttct tctgttagtt cgatatattt caagacagca cctgttcgtt ttgccaactg
     5221 ctgccacggg ataacattag aatgatgttc catgtaagag agcactatct cgtctcctgc
     5281 tttgatatag cggtcgccga aggttctggc tacccaattc aagcttgtcg tcgtaccacg
     5341 agtaaacagc acttctgctg tttcttttgc atggatgaac gcccgtactt tttctcttgc
     5401 ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg acaccgcgat gaacattggc
     5461 gttatcgtgt tcgtaatagt acgctaatgc ctggagtact tgttttggct tttgggtcgt
     5521 cgctgcatta tccagataaa ctaaaggttc atcattgacg tcttgaaata agataggaaa
     5581 atcattgcgt agctttttag catcgatcat ccattcaact tcccttcgat tacttcgact
     5641 aattctttcc gaacttgttc tacagggatt gctgttaaga cagaaccgag gaatcctcga
     5701 atcactaagc gctctgcttc ttctttatgc aatcctctac tcatcagata gtacatttct
     5761 tctggatcaa ctcgtcctac actggcagca tgtccagcag ttacttcgtt ttcatcgatc
     5821 aaaagaatag gattcgcatc accacgtgct ttatcagaaa gcatcaagac acgactttct
     5881 tgttgggcat ctgcaccttt tgcccctttc aagatgtggc cgataccatt aaaggtcaat
     5941 gttccttttt ctcggatcac tccgtgttgc aaaatatgcc cgatcgtatg aggggcttta
     6001 ttggttactc gtgtatcgat tccttggatt tgtctgcccg agctgattgc cacgattttg
     6061 acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa agtcagcgat cacatgaccg
     6121 tcattcatga caccaattgc ccagtcgaca gaagcatctc gtaaaatgtg cccacggcga
     6181 ttcatataag aagtaatatt ttctcctaat tgatctacgg ctgaatattt gatttttgaa
     6241 ccagctttag cgatgacttc cacgatgata ttacctgatg attttgctac ttgttctttg
     6301 gttgtctgga aacgttccag ataagaaaat tcactatgtt catctgccac gatcaacaca
     6361 tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga ataaggactc gagtggttca
     6421 tcgatcacga catttttagg cacatacagg aaaataccgc tgttcataaa agctacatgg
     6481 agtgcagtca attggtcttc gttcatgttt actgcttttt tcatataata ttcttggact
     6541 agttctggat attcgatcat tgcagtgaac aagtcggtaa aaatgactcc tttttctgct
     6601 aattccacag gcagttgttc aaaaatcgtc catgaacctt gttggacaag cacaggattg
     6661 tctttcattg catcaaatgc cggaatcgtg cctgtttcac tgatggtttg tgtttctttc
     6721 acatcaaata acgaccagcg atggaatttc acccgatcga tatgcggcaa tggcagttca
     6781 tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc aatcaggttc gcctttactt
     6841 aaagaaaaat catttaccgc gtcaagataa tcgagccagt ttttttcttt cattcttgct
     6901 cccccttacg cttcttcttc tttatagtcg attcctaatt ctttactgat accagcgtag
     6961 ccttctgctt ccagacgttt agctaggtca gcattccctg tcatgactac tcggccttcc
     7021 atcatgatgt gaacgacatc aggtgtgata tagttcaata atcgttgata gtgggtgatg
     7081 atcaatgcac cgaaattgtc tccgcgcatt tcatttactc ctttagcaac gactttcaaa
     7141 gcatcgatat ccagacctga atcgatttcg tctaaaattg caaatgtagg ttctaacatc
     7201 aacaattgca ggatctcatt ccgtttcttt tctccgccag aaaaaccttc gttcagataa
     7261 cgttcagcca tttcttctgg catatttagt aaagccattt tttcatctaa ctttttcaaa
     7321 aatgccataa cagaaatttt gtcgttttca tcacgtttcg cattgatcgc tgcacgcatg
     7381 aattccgcat tcgtgatacc tggtatctca cttggatatt gcatagctaa aaataagcca
     7441 agtcgcgcac gttcatctac ttccaagtct aaaacatttt tcccgtcaaa caaaatttcg
     7501 ccttctgtta cttcatagtt tggattgccc atgatagcgg cagataaggt agattttcct
     7561 gtgccgttag gtcccatgat ggcatggatt tctccggttt tcattgttag gtttacgcct
     7621 ttaaggattt ctttttcctc gatcgacaca tgtagatttt ttatttctaa gacagacatg
     7681 cattataccc ccaaattctc tttcgttctc actcatttta gactaattga gaatgaagcg
     7741 caatgacctt ccttactaaa atcaaatatc ttttatttat tattaaacaa ttttgatttc
     7801 taaattagaa taggtataaa aaagatgaga aataagccat tacagactta tttctcatct
     7861 tttttgttcc ataccttatt ttgcatcagc ctttactggt ttaacagagc cgccccattt
     7921 gtctgtgatc cattcttgga cttctttgcg gtgaagcact tcaatcagtt ttttgatttt
     7981 gtcactgtct tcatcacctt tacgagtcgc aataatattc acgtaaggag agttgtcctt
     8041 ttcaagcaat acggcatctt ctaatggatt caaccctgcg ccgtaagcga agtttgcgtt
     8101 gattgcaact aggtcacctt catcattttg gtacgtagaa gctaaaagtg atgggtcgat
     8161 cgtatggttg aacttcaagt tcttagggtt ttcttcaata tcatcgaatg ttgctgtttc
     8221 cagatctact ccatctttga ctttgactaa gccagcatct tgtagaattg tgatgatgcg
     8281 tccccagtct gattcagaat ttgaagtaat gattgttgca ccatctttta attcggaaac
     8341 atctttgact tttttagagt acaagcccat tggttcgatg tggacagcac cggcattgac
     8401 aaagtcatat cctttttctt tgatttcttt gttcaaataa ggaatatgct ggaagtaatt
     8461 ggcatcaatg tcgccttctt ccaaagattg gttcggtaat acatagtcat cgaatttgac
     8521 gatttcaaga tcgattcctt catctttcaa taatggtttc acatgttcca aaatctctgc
     8581 gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg tctgctttat ttgctgaatc
     8641 tttgctgtcg ctgttgttac cgcatgctgc taatcctaca gtcagcaaaa gcgctgctgc
     8701 aaatccaaaa attttctttt tcatatccta catcccccta tatcctatta tcttttgtct
     8761 gttttcttag ttaagaaatc accgattcct tgaacgataa atacgattag taaaataatt
     8821 aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt agccttcctg ccaagcaaga
     8881 cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag taaatccgat catcgagatc
     8941 gttgtaactg ttaatccaga aatcaatgcg ggaatacttt caggtatcaa gaccttccag
     9001 atgatttccc agtaacttgc tcccattgca tctgctgctt ccagtacacc agggctgact
     9061 tcacgaaaag caatctctac tagacgggca taaaatggtg ctgcggataa aacaagagct
     9121 ggaatggctg cttgcgcacc tagcatagtg cctacgattg ctttagtaaa tggaatgatc
     9181 aaaaccatca ggatcataaa tggcgttgac cggaaaatat tactgatgat cgatacgatt
     9241 ccataaatga ttcgagcata cggtttgttt ttgcgtccaa tagagtaaag gatcaatccc
     9301 aaaactaatc cgatgatcgt cactaagacc attgagatta atgtcatata tagtgtatca
     9361 aggatagcct gattcatcgt ttccgggttg atttgtttaa aatctagata tgtttctgaa
     9421 aagcttttat ccacgatgga tcacctcaat tcctactcct tgttctttga tgaacacttc
     9481 tgcttgatct aattctttgg tttcaccagt catcaagaca gtcaatgaac caaaggaacc
     9541 ttctttcact tgtttgatct ttccatacac aacgttgaca tcgatgttat agcgtcgaat
     9601 cgcttgggaa atgactggct cgttggcatt tctttcgcta aaggtcaaag ttgccaaacg
     9661 gccgcttgga ttttctttga tgagttcagc taatagttct gtcgaatcag cttctggttc
     9721 gatgtcttgt tgtacaaagc gtttggttac ttcttttttc ggttgtctga agacagtcaa
     9781 tacgtctcct tcttctacga ctttccccaa ctccatcaca gcgactttgt tgcagatttt
     9841 gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc aaattcaatt tcttattgat
     9901 cgctaacaat aagtctaata cttcttctgt tgtttgcggg tccaatgcgc tagtagcctc
     9961 atcacaaagc aggatctcag gatcatttgc caatgctcgt gcgataccga cgcgttgttt
    10021 ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt ccttctaacc cgactaaacg
    10081 caacagttct tctgcccgtt ctttacgttt gctctttggt acgccagcta attccagcgg
    10141 cagctggata ttttcaagta cagttcttga ccataacagg ttgaaatgct ggaagatcat
    10201 accgattttc tttcggaatg tacgtaattc tttgtttttt aattttgtaa tatcttgatc
    10261 gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca ttcaggagac ggactaatgt
    10321 actttttcct gcgccagaat agccgacgat tccataaata tccccttttt caacgtttag
    10381 tgaaacgtca ttgacggctt ggatgtttcc gtttttccca ttgaatgtct ttttgacatt
    10441 cgttagttgg attaatgaca tgccattcac atctttctct tataattttt caaacaaaaa
    10501 agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt gaacttccgt gttaccacct
    10561 tgatttaccg ttacttcgca ataacagcct cttccagtac ttggacttag cttctttccg
    10621 tatactgtcg cgctgtaacg ggcactcccg tagtaagttt gcttacgctc gcttactctg
    10681 ctctaagacc atcttccatc tcattttcgt tacccatttt cagcaaacag ggctctcttc
    10741 aaacgtccgt gacatgtact cttcttttca aagctttctc gtttcatata ggattgctta
    10801 tgattatagc aactggaaga atcagtgtca acttttttca agtgattttc tgattttata
    10861 attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa gatccacgca ttcatttcat
    10921 ctgaatcatt caagatattg atatcttcac cgattttatc attaacagaa gaaactgtac
    10981 ccgttagcgg gctggcaaat tcattgaccg ctttttccgc ttcaacttcg atcagaggtt
    11041 catctttttt tagcgattgt cctgcttttg gcagattggc aaacgtgatg tctccaagtt
    11101 cttcttgcgc ttcatttgtc aaaccaacac agtattcttt tccattaaat aagatccaaa
    11161 ggttgtcctt tttcttcaaa tgcttgcctg ccattttata tcagttctcc ttcataaatt
    11221 ttttcattga atccaattgc aacaagctgg tcctctcgta agatcagtgg tcgcttgatc
    11281 agcataccat ctgtcgataa gacttggctc gcttttgaag ccgtaaactc gcctacttga
    11341 tctttcagtc caagttcccg atatttcatc ccgcttgtgt tgaacaggcg acggattggt
    11401 acttgactcg tttctagcca agtctcaatc gttgaggcct tcggtacttc tttgatcata
    11461 tcaacggttt gataactaac gccatgctgg tctaaccacg ctttggcttt tttgcatgtt
    11521 gaacatttcg ggtaccagta aaaggtgtac aattttctta cctccttgaa gatcttgcag
    11581 gtgcttcatt ttggtatctc atcgataata tcaacccgat accgatcatg ttccctaaaa
    11641 tcgaggatcc tccctgacta atgaaaggca atgggatccc tgttaaagga agtaatccga
    11701 tatttgctcc aatattctca aagacatgga acagcagcat catgatcaaa ccgcttgcga
    11761 tataggcgta aaattcatta ttcgtatcga aacacacccg aatcatacga tagattaaaa
    11821 taaaatacaa gaaaatgacg aatgcaccac cgataaagcc gaagttttca ccgataacgg
    11881 taaaaatcat atcggactca cgaacaggca catagatatt gcttacgttg aatcctttac
    11941 caaacaagcc accggtacca attgccaaaa gtccttgggc cggctgataa ctcattcctg
    12001 acgtatcatg gaaagggtcc agccatgaat caatacgtgc aaactgataa gatttgaatc
    12061 caactttata aagcaagtct cttccgcctt cagtcgtaac caagaagatc gttcctgctc
    12121 caacgactac tgccaagatt actacaggtg cgatgatgcg ccaagagatt ccagacatca
    12181 ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt tccaaagtca tcttgggcca
    12241 tgatcaatgc taaaaccggt attgtcacga tcaagagctt cccaatcagc caaaagtccg
    12301 atttcatcgt ccgttctttt acttgcgtat tatgtttagt tacgatcagt gccatcatca
    12361 agatgtacgc aatcttcatc aactctgctg gctggaaagt aaacgcaccg aatcgaaacc
    12421 agtttttaga ccctgttgaa gcagctaaat tctgatcata gaatttgagc aagagtccca
    12481 ttaccactaa ccccaaacca taaaggtaag gagtcagttt ccagatccat ttggaattca
    12541 aatgcatgat gacaacgatt gctgccgctc caactaaata ccagacaact tgcatcccta
    12601 cgcctttcaa gactgctgga ttgttcggat catggctaag tgctacatat aatgagagca
    12661 gaccaatcaa acataacaga aagactggta aaatcacgcc ataatcaata cgattatcgt
    12721 tcgaagcttt taattttttt tccatgtttc atcccttttc ttaaaaacca tactgctgat
    12781 tattatagcg gtccagttag ataatggaaa gtctttccat agacatttgg gaaaaattaa
    12841 ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa agcctactgc ttatctttag
    12901 tgtatcatag aacttttttt gatacatgca attatctacc agctgagatt ctcctcgctg
    12961 tgagaagttt ctgtgctttc attgtatcga aggatctctt cggcaatctc tagcagctga
    13021 tcagaaacca gattggttcg ttggatgaaa taaaaatatc gatggaaatt caacggagtc
    13081 attggcacat tttctggaac agagcgctta gacaaaatcg agcgaccgat tccttccttc
    13141 agcaacgcaa caattacatc gttacttttc acgatcatct tcttatcttg gatattcatc
    13201 tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt ttgcttctct tacaagccac
    13261 aatttgcttt ttgggtcccc agcgatgact agctggtctt ccatcaaaga gtatctttgt
    13321 acgccggaag tctctaatgg cttttcgata aaaccaaaat cggattcatg tttcgctact
    13381 tgttctaatg attcatgaga attcgtgatt tcgatctcaa aatgcacttt aggaaacctt
    13441 ttgatcagat gtttcatcaa ctgaggcaaa agatagacag caaaggtgtg ggaagcaacg
    13501 atccgacata cttcttcttc attttgttca ttgagcaatt tttgctgagt ttcttcccat
    13561 tcttctaaca aaaccagtgt ccgttcatat aataagtcgg cttgcttagt tggaagcatc
    13621 tcttttcttc cgttacgttg aaaaagatcg atattcaatt cccgctccaa ctgtttgatt
    13681 tgattcgaga ctgctggttg agataaaaac agtacttctg ctgctctgga aaaatttctt
    13741 gtttcatata ctgctcgaaa agtcgttaat aacttaaaca tggcaccact tccataactt
    13801 tttatgataa ttactataaa aactatttga tttaaatata caaaaaacca tctataatag
    13861 aaatgtacaa caaaacagtt gctgatccaa ctagtaactt agatgaagac aatcagctgt
    13921 ctgcttgctc gtaatcatac attcctattc attttacaat gtattcgttc atcttggtac
    13981 taaaatctat taaataaaaa ggagaatatt atatatgaag aactttttga aaattattcc
    14041 gatcccgatc tgcgggttga tccttgggtt agcttccctt ggaaatctgt ttaaagacta
    14101 tcaccatgtt gctttaggca atctaactgg cggtatctct atgattctta tgatattcat
    14161 ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa acgttgcatg atccaatcat
    14221 cgcctccgtt tcaccaactt ttacgatgtc actcatggtt atttgtactt attttgtttc
    14281 atatgaaagt attgcaccaa ttgtcaaatt catttggctg tgcgctgtta tcttccaagt
    14341 gatccttgtc ttttatttca attatcacca tgttatcaaa gctgatcttt caattgaagc
    14401 aatttatcca agctggttta tcatttacgt tggatttggg gtcatcactg taactgccgg
    14461 gaacttcttc ccacttattg gaaaaatttt cttttggatt tcattgatct gctatgcgtt
    14521 cttattgccg atcatcatca aacgtgtctt tttcgtaaga aatatggctc atccgacacg
    14581 tccgctgatt acgatcgttg cagcacctgg ttctctttgc ctaacaggtt atcttaaaaa
    14641 ctttgctgaa cctaattttt atcttgtcgt agcattgttc gctttatctc aagtattgta
    14701 tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt cgtttttatc caagctatgc
    14761 agcctttaca ttcccgcttg ttatctctgc aacagctata tttactactg ttcattactt
    14821 caacagttta ggtatctata gtagcgtatt ggatttctta aaagttgcgg aatgtctgtt
    14881 tgcaagtgta gttgtcttct acgttcttgc tcattacgta cgtttcttaa ttaaagaaca
    14941 tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa taatcgattc aataggttgt
    15001 gaaaaagctg ttctgcatca agtaataaga acggccaaac aaaaacagct gttcatgttt
    15061 ttgtactggt tgggcttatt accgcagatg caagcttttg aacaccgttt accaagaggt
    15121 cgtaaaagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa
    15181 tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg tttattcgaa
    15241 acaagaggtc gtacaagact tttgtcacga cctcttgttc ttttgtcaaa atactggttt
    15301 agtgctacta tgttcgagta tgcttttagt atttattgac gaaaggaacc gatcaaatga
    15361 aatcattgtc acatccaata aaatctgtaa aggattcagc ggtccgcgaa atcttacccg
    15421 gtttactgct ttcttttgcc atcgctgcct tcagcaggct tcttgctatt tatctgccac
    15481 agttaggtgg agcgactatt gctattttat tgggtatttt attaggaaat acttttttta
    15541 aacagcctgt tctagcaatc ggcacaaaat ttgctgaaag caaactatta gaattctctg
    15601 tttttttact aggtgcaact gtcacagctc aaacgattgc tcaaattggt gtaaaaggaa
    15661 ttgtttttgt gttactgcaa atgtcagtca ctatcatcgg tgcttacttg attggtaaaa
    15721 aactgcaatt cagcgatact tcttctttga tgatggctgg aggaaacgct gtttgtggtt
    15781 cttctgctat tgcttcaatc gccccttcca tccatgcgaa agaggaagaa aaaggacaga
    15841 tcatcacact agtcaatcta ctgggtactg tgatgatgtt gactctccct tttcttggat
    15901 tacagttgtt tggtggaaat ctcattgaaa aaagtgcact tttgggtggt acactccaat
    15961 ctgttggtca agtagtcgct ggggcaagct tacttgacaa ttctgttgtc gaattcgcca
    16021 tgctatttaa gattctacgt attatgatgc tcgttgttgt tgtctatctg tttgaaaaat
    16081 tcatgcacaa gaaacaacca gctctgtttg aaacgccaac tgatacgcag aaaaaacgaa
    16141 aattaccgct tccttggtat gtattagggt ttgtgatctt ttgtttggtc aacagcttgt
    16201 tttctttacc tgtagttatt gaccaatcgg cacattttat cagcagctgg tttgagatca
    16261 ctgctttagc ggcaattggt ctgcgtttag actttgcaaa attcatgaaa gaaggtacac
    16321 gcttcttatc ttatggatta gctgtcgggc ttctccaaac agtgatggct ttactattta
    16381 tttggctgat caaagtttag aatgaacatg aaaaaacagt tgtgattctc gcatggaaca
    16441 atgtccgaat cacaactgtt tttaattttg cagatatcct tctaacaaca acaattggcg
    16501 tttgatttca accccatcta ttgcgtttcc catatatcct gtcagttttc cattttgccc
    16561 gatcactcga tgacaaggaa taacgattgg caatggattg gttcgatttg cctgcccaat
    16621 cgctcgcaca gctttcggac tatcaacagc aacggctact tctttatacg ttcggatttc
    16681 tccataagga atggtcgaca aagctttcca gacattttgc tgaaaaggcg tacctctctt
    16741 tatagataaa ggaacatcaa attcttttct ttttcccgca aaatattctt caaactgttc
    16801 ttgtgtgcgc gttaaaattc gctgattcgc tgattcgctt tctgaaattg gctgaaaaga
    16861 tacagcagtc aatccggatt catctgcatc taaccataaa aagccgatag gtgtgtgtat
    16921 tttcattttg tttgctccta gtcaatagac tggaacttcc tcctttttct ctatccaaaa
    16981 ctacttgcca actaataaaa attgttctta aaagtttgat aaaccgagga aacatggtaa
    17041 actaaatacg ttagtttaca aaaagaatac ctgttctttt acgtaaaatc aaattttaaa
    17101 ggtgttattt aaggtttatt aaattttgaa tgtaaaccat atataaacat acgattgaaa
    17161 ggatagaaac gatgaaagta tcaattgttg tcccatgttt taatgaagaa aaaactgttc
    17221 ctcttttttt tgatgaggtt gaaaaattca aaggtgccca tgagttcgaa tatttgttta
    17281 ttgacgacgg atcaaaagac ggtacgttag cagcaatcaa gaagctagcc tcagcttatc
    17341 cttatgtccg ctttgtctca ttttccagaa attttgggaa agaagcggct ctgtacgctg
    17401 gattgcaagc ttctacagga gatttagtga ccgtgatgga tgttgatctg caagatccgc
    17461 ccgaactgct tcctgagatg atcaaccgga tcactacttc tgatatcgat tgtgtcggca
    17521 cccggcgttc gactcgtgaa ggcgagcctg cgatacgcag tttctttgcg aaaaaattct
    17581 accagttgat caataaaatc agcgatacag aaatggtcga tggcgctcgt gatttccgct
    17641 tgatgactcg tcaaatggtg gatgccgtcc tagaattaac agaatacaac cgattttcaa
    17701 aaggattatt tagctgggtc ggatttaaaa cagaatatat ttcttatgaa aaccgcgaac
    17761 gtgtggctgg cgaaacatct tggtccttct ggaaattatt caactactcg attgatggta
    17821 tcgtgaactt ctcggatgca ccgttaacga ttgcttcatt tatcggcgct ttgtcttgtg
    17881 tcggttctgg tattgcaatt ttgttcatta ttttgcgggc attgttgttt ggtgatccta
    17941 catcaggatg gccttccatg gtctcgatct tcctgttcat tggtggaatc caattgttat
    18001 gtcttggaat catcgggaaa tatattggga aaatcttctt agaaacgaaa cagcgtcctg
    18061 tttatatcat aagagaaaca gaaaaagtga agccaaatga attgaagcct caccaataat
    18121 atacgtcaaa tgaagtaaac atttttttaa atgaaaaagg actgtgacaa aagtcttgtc
    18181 acagtccttt tttccgaata aatggtgttc cagaagtagc ttcttcggaa ataagccgaa
    18241 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    18301 acttctatca caacctcttt catatcttat tacttcttca ttgctgcttt tagtgctgct
    18361 gctaatgggc tttcttctgg ttcttctgct tgtgcatatt ttttcagcaa gcgtcgttct
    18421 tcatgtttgg tcattttctt cttctgctct tttttatcaa gcaatttttc tgtcgttcca
    18481 tcgtatttac aacggaaata agcaccgttt ttaccttcga tgatttccat tttccggtga
    18541 cattgcgggc atcgatgatt agagactttt ggatcttttc ttctgcggta gttgcaatct
    18601 gaattggtgc agacatagat tttgccgtca cgtgtgtttt tctcgcgcag attggcccca
    18661 cattctgggc atttcttttg ggtaatcgag aaatcttgat attcttgttt gcttgatttg
    18721 atctcattca ccaagcgttt tgtttctttt tcgattccat tcaagaaggt tcctgcttgt
    18781 ttctttcctg cagcaatagc ttcaaggtct ttttcccagc tttctgtcaa atctggtgtt
    18841 actaaagagg gatttaccaa tactaataat tgcttacctt taggagatac ttgcagtcct
    18901 tgaggggtac gttccatcag ttcagatttg attagtttct cgataatctc tgcacgcgta
    18961 gctggggttc ccagactgta tttttccatt tgaccaagca agcttgcttc tgacaatggt
    19021 ttaggcggtg cagatagttc ttttttgatg gcgaaatcgg gattgattct cattcctttt
    19081 tcccatttga ctgtttccgt ctcagtttca gtttcgcctt tccatcctgc ttggacgaca
    19141 cgattttgcc gaaaaatgaa tcgttcttta ccaaattcaa ccgtaacttt tgtttggctg
    19201 actttatggg gttgagcaaa cagtcctaaa aaacgagaga caatcatttg atagattttt
    19261 cgctcttcat tacttaattt ttcataacgt gctctgtttt cagttggcaa aagtgcatgg
    19321 tgatcggtca ctttactgtc ttggaatact tttgtctgtt ggacgatggc tccgtttttc
    19381 aaatactgct tgacctcagg agcaaagtcg ctgactgctt gcaatcgttc tttcatcgta
    19441 cttttcatgt catttgtcag atatttgctg tctgtacgcg gataagtaac gatcttatgg
    19501 gtctcataca aactttgtac aagacttaat gttttctttg cagaatagcc atagcgttga
    19561 ttggcttctc tttggatctc tgttaaatca taaggcaatg gcgcagattc cgtttttaat
    19621 ttttcttgga cttctgtgac agttcctttg cttgtactga tcaattgaac aagtttttgt
    19681 gcttcttctt gtgtttttaa agcaaattga tttttttgaa gcattttggc ttgttctttt
    19741 ccagcttgca acgtgatcgt gaaatacgtt tgtggacgga actgctcgat tttcttttct
    19801 tggtctctca ctaatgctag tgtcggtgtc tgcacacggc ctgctgataa attatccgcg
    19861 tacttcacag ttaaggctct ggtcacattc aatcctacca gccagtccgc ttttgcccgg
    19921 gcaagagcag attcatacaa tgcatcatat tctttcgcag gtcttagttg cttgaatcca
    19981 gttttgattg ctttatctgt ttgagaagag atccataacc gcttcactgg tttattgaaa
    20041 tgcacatact ctaagatcca gcgagcaaca agttcccctt ctcttccggc atctgtcgca
    20101 atgactgctt ctgagacatc ttttcgctga gccagctgct tgattgcttt taattgatgt
    20161 cctgttttag gaagtggttt gattcctagt tttttaggga tcattggcaa cgtgtccatt
    20221 tgccagctct gccattcctt attcagatct tccggcattt tcaatcctaa taaatgacca
    20281 agggcccaag taacgatcac cttgggtcct tcataataat ttttgtgctt ttgattagca
    20341 ccaagcacct tgcttaggtc tttcgcaacg cttggttttt ccgctagaat tagttgtttc
    20401 ataaaattcc tttctgcttt ctactgtgat gggctgatga tttttcaaca acgcaagtcc
    20461 cttgtattgc tgtaaatcat cgtcacactc ttcacaccag cgctcatctc cgtcattcca
    20521 aaaggcgctg agaaagttac tttttgcatt gatatattca taattttgat ggatttcgtt
    20581 ccattgtttt tcgtacgctt gtcgtgcttc ttcgaaagac tcgaatgttt cttcttcttc
    20641 aatatcttct tcccagtctt caaaaaacca ccaaggctca ttgtccccga acatggtaat
    20701 tacttgatac atactcgtgc tccttctttc agcttttcta gactcatctt agtcactttt
    20761 caagaaagtt gcaagaattt tctcttataa aagggtcatt taatgaacat cttaaaattg
    20821 ttacaaatga atctgattgt ttaaggcaaa gtagcgataa agttattgat catccaaaaa
    20881 tagctgtctt ctggatcgat tgttggttct tttgtaaagt atcctaaaaa acgtaaggaa
    20941 agataaccgt ggatcatact acgaagttcc ctgcttcgat gaactttttc ttctttcgtt
    21001 aattgaaaag cctcaaatat ctgcaaaatg atctgattcg tttcatggat tccatctaat
    21061 aaaacagctt cattggtatg cggtacactg atcagtaact catacactgc ttgatttgaa
    21121 aaagcaaagg atcgataagt ttctgcatag atctgcagtg cttcctgacc actttttccc
    21181 acaagagccc gtcgcagctg gtcattaagt tcctgcagaa gtttcgctgt tagcgctgtt
    21241 ttgacatcac gaatattttt gaaatggtta tacagcgagg gagccttgat atccaagcgt
    21301 ttggctaaat gctgaaaggt tatttgctga acatccatct cttctgctag ttctcgaaag
    21361 cagtcgatga ttttctcttg agacagattt ctttttttca tttccatgtt tcctctcata
    21421 aaataaatcc ttcactcttt cagtttacca cgaaaaagaa agagaaaaaa gagactttca
    21481 ttgataaact acatattgta gtttataata aagagctgta gatgaaaatt ttcagaaaga
    21541 aggagtctaa ttggctgcaa gaataaagga aaaattattt aggaaaagag aaacttggga
    21601 acaaaacttg tttgtcttat ggtttggaac atttatggct ggtatcggat ttagtttagt
    21661 catgcctttt atgtcacttt acatagatac gttaggtaat tatagtacgt cacaattgaa
    21721 cttttggagc ggattgactt tttcatctac tttcttggtg actacgctta tctctccttg
    21781 gtggggaaaa ttagccgacc aaaaaggaag aaaattgatg ctgttgcgtg cttcattagg
    21841 aatggcaatc gtcatttctt tgatgggttg cgtgactagt gtgtatcaat tagtcgcatt
    21901 gcgtctgctt caagggattt tttctggtta tatcagtaat gccactgccc ttgtcgcaac
    21961 tgggacgcca aaagaaaaaa gtggtcaagt tcttggaacc ttagcaactg gttctgttac
    22021 aggaacatta cttggaccgc ttcttggcgg gatctctgct tcagcgtttg gctatcgccc
    22081 tacctttttc attacaggga tcatcttatt tttcgtcttc ttgttgagtc tgtttttcgt
    22141 ccatgaaaaa tttgtcccag tgaaaaaaga aaacatggtt tctgccaaac agattttcaa
    22201 agacttgaaa tatccacacg tcgtcatcgg tatgttcgtc acaacgatga tcatccaagc
    22261 atccaacaat tccattagtc caatcatcag tctgtacatt cgccaattgc tgcatggaca
    22321 tgggaatgtt acgttgatca gcggaatcat tgcttcgata cctgggattg ccactttgat
    22381 agccgctcca agatttggtc gcttaggtga caagattggg agtgaaagaa tacttgcgat
    22441 tggtttgggt tttgcgattc tggtttatat cccaatggca ttcgtcacga atgtttggca
    22501 attagctgcc ttacgttttt tgattggtat ctctgatgct tgtctgctcc ctgctgtcca
    22561 agcattgatc acaaaatatt cgcctcatca agctgctgga cggatcttca gttataacca
    22621 atctttccaa gcaactggta acgtagtagg gcctatgatc ggctccagtg tttccagtgc
    22681 atttggttat cgcggcgtct ttctttcgac ttctcttctt gttttggtca acttcttgct
    22741 tgtacgacac aatacacaag agatccatca gcaagaaact gcagaagaaa aagcttcagg
    22801 agtctccata gcttcagggc actctccttc tcatagacaa taagcgtaaa tgaaaaacac
    22861 tgcttgaaca gttgtcacag actgtccttg cagtgttttt gtttaggcct gttttttcaa
    22921 gaactcagat aagaggaata atcccttttc caaggtttct tttttacaac agtaacctag
    22981 tcgggcatgc ttcggtaaat caaaggcact tcctggtact aatagtactc ctgtttcttc
    23041 taataaattg atacaaaatg tctggtcatc ttcttcgatg gctaatttaa tgaaagaagt
    23101 agagacgtaa ttgggagcga caagttcaac tttcggttca ttggctaccc attgttttaa
    23161 aatcacaagg ttctctgtaa tgatcttttt gtttcgcgcc aatatttttt ctttattctt
    23221 caatgcatgt acagcaagat catctgacag gactccgccg caaatcatcg tgtagtctcg
    23281 atactttcga aaaacttctg ccaactcagg tccagtagct gtccagccaa tacgaatacc
    23341 tggaatggag taggttttgg atagggaatt ggttgctata cctttttcat agacatctac
    23401 gatcgacaag aattcacctt tatccgttaa aggcgcatat acttcatcta tcaatacata
    23461 tgcatctact gtacgagcaa tttctgcgat tttttctaaa gttttccgat ctagcaatgt
    23521 gcctgtagga ttgtttgcac tattcaaaca gatgattttt gtttcaggtt tgactaaggc
    23581 ttctagctgt tctaaatcaa actgccaatc ttcttcttcc ttcaaatgaa caaagtcaac
    23641 agcggcacct aaagatttgg ggatgtcata taattgctga taagtaggaa gcatcgaaac
    23701 aacatggtct cctgcttcca ctaacgcgta tagagctaaa agattagcac cagtcgctcc
    23761 attcgtttgt aagatgtttt ccggatcgac ggtttgataa agagaagcga cttcttgttt
    23821 gaaaacatct gatccttcga tccagccata gtccaatggt tcgtttttgc gtttttcgaa
    23881 aaatttactt acgctagtcc catctaagcc aacgagttcg tctaatgtca atgcttcgat
    23941 cgagctttgt gcaatatcat ataccgcttt cttttcccat ttatttagcc attcttctac
    24001 accgaaattt gcgattttca tgtttctttc ccccttatat cttgatccag cctacagcaa
    24061 gcaacccgat tgaaagcagt gcggccacac aaatcagccc aatcattact ttttcttttc
    24121 ccgtaatttg atgtttggtt tcacgacaag cgatccagta gaagcaaaaa ccaggaagat
    24181 aggcaatact acataaaagg acataagaaa atcctgctaa aatgatgccc atcaattcaa
    24241 aaactacagc aatcccaccg atcactactt gttttttgat tccttctttt aaagaaaact
    24301 ggacttgata gatcccgaca agtagataac aaatcaaaat tgccgctgta cataaagaat
    24361 aggcaaattg ataggcataa tcagtaacta agaaagtcaa taagaataga ttagtcaaga
    24421 ttgcagtaac aactaacgaa taagttgggg catttttttt attcgttttt ccccaacctt
    24481 tcggcaacag accgtctcga gccatcaaca tcgttgtttc tgctgggagc atcgtccaag
    24541 acaaccaaga accaatgatc gaaataatca agccgatatt gatcagcgct gctccccaat
    24601 ttcccacgat tccttttaaa atatttgcca tagcaggttg tgaaatagtt gctaactctt
    24661 cttgagataa gacgccgtat ggcaagatcg aagcaaggat atagataaac aataaacaaa
    24721 gcagaccaat gatcgatgct tgctgggctt ctgaacgttt cttcgcgcgg ttggctaaaa
    24781 cacttgcgcc ttcgatccct acaaagaccc acatcataac catcaaacaa cctttaatct
    24841 gcaaccagat cgttccttgc tgccctgttc cattcactag attatcagat acgttacccc
    24901 aaaaatcagc agtgaatata ttcactttaa atgcgataaa tacaaatatt aaaaatagga
    24961 ataatggcac taatttacag atcgtcacga ttgtattgat gacgctcgct ccttcaactc
    25021 cattgtttac taataaagtc agaccccaaa taaaaatact tgcaaataaa atagaaggaa
    25081 cattttgtcc acctttgaat atcgggaaaa attctcccat ggcgctcatc agcatcgttg
    25141 caaaagcaac atttcccagc cacgcagaca accagtaacc ccaaccactg atgaagccac
    25201 ctaatttacc aaatgaagct tccgcgtaac cgaaaatacc accatctaga tctggacgct
    25261 tttctcctaa atgattcagc gataatacca aagctaaaat accaatccca acgattatcc
    25321 atgaaaggat cgctggccct ggagctgcag aactagccag atcacttgtg atcccaaaca
    25381 caccgcttcc gattgacgaa ctgacaacca gagcaaccag tccccatttg ctaatacccg
    25441 tcgatttctt ttccatttcc atcaccctca cttataaaat tgaacaaatc acttgcaaaa
    25501 gattcccttc tattttgcaa ctgctcttgc ttaacaaaaa gcaactttat tcatattatt
    25561 ggatattatg aactttttac acaagtattt caagtataaa atgaataaaa attaatttta
    25621 ttgcaaaaaa acagctatgc tatccaaaag aaaaggtgtt tcttttgtta gccatagctg
    25681 ttttgattca tttaatatcg cttaaacgta ttgtaaaact tttgtttctt gatatgcgtg
    25741 gtcgatcaaa ccaccaccca gacattccat tccatcataa aatacaactg cttgtccagg
    25801 agtgattgca cgtacaggtt cgtcgaatac gacttctgca cgattatcat ccaataagcg
    25861 aactgtcaca ggaacgtctt gctgacgata acggaatttc gctgtacact tgaattcttt
    25921 tggcattggt tcattcgtcg taaagtggat ttcacttgca tccaagcttg tggcatacaa
    25981 tgcaggatga tggaaccctt gccccacata taatgtgttt gtcgctagat cttttcctac
    26041 aacaaaccag ggttcttgcg aatctcctcc gccaccgatc cctagtcctt gacgttgacc
    26101 aatcgtataa tacatcaagc cagcatgttg tcctttgact tcaccatcaa gagtgaccat
    26161 atttcctttt tttgctggca gatagttgct caaaaattgt ttgaagttct tttcaccaat
    26221 aaagcagatt cccgttgaat cttttttctt agcagtcgca aggcctgcgc gttcagcaat
    26281 tgcccgtact tctgattttt ccatgccgcc tagagggaac atcgtttttg aaagctgttc
    26341 ttgtgataat tggctaagga agtaagtttg atctttatta ttgtctactc cacgaagcat
    26401 gtggaccacg ccgttttcat cgcgagtgac ttgcgcataa tgtccagtag ctacataatc
    26461 ggcgccaaga tccattgcat aatccaagaa tgctttgaat ttgatttctt tgttgcacat
    26521 cacgtctggg ttaggtgtac gtcctgcgcg gtattcggct aggaaatatt caaatacacg
    26581 atcccaatat tctttttcaa aattcacaga gtaataagga atcccgattt gagcagctac
    26641 tttcgctaca tccttataat cttccgtggc cgtgcataca ccgttttcgt ccgtgtcatc
    26701 ccaatttttc atgaatattc cgatcacgtc gtatccttgt tcctttaaca aaagtgctgt
    26761 tacagatgag tcgacaccgc cgctcatccc tacgacaacg cgtgttttgc tgttgtctgt
    26821 catgatatca ccatcatttc aattagattc tgaaacatct acgagttgaa ggtcgcaact
    26881 tttcagtatt ttacattata cgtcatattt ctgaaaattt catcccttaa ttatcaagtc
    26941 gtaaaaatac tgttctactg gacctattca gtacacaaac atgatagtca accaaaaaat
    27001 cactcatctt ttgagcattc gatataaaaa attggcatac tcataaaaag ggccagcctt
    27061 tcggcaataa ggaataattt tataaattgt gaaaaactat ttttcaaaat tgcttcttat
    27121 cgcttaaagc tgcacgtctc atttacaacc ttttcttttt agatgttatg agttgctcag
    27181 ataagtgatt ttgtttactc taagaaaaaa gggaggcaaa agatacatga attactttat
    27241 cagtgatatg cactttttcc atgaacgctt attaggaaaa aacgactttg ctccgcggct
    27301 ctttgcaaat gtggatgaaa tgaaccaaca gttgatcact agctggaatg cagtaataaa
    27361 agaaaccgac catgtttatc atctaggcga ccttgctatg catccgcaat atgaaaaagc
    27421 aaatgcagaa attttggaac tggtcactca actgcaagga acaatccact tgatcaaagg
    27481 gaaccatgac agtcgcgctt ttttccgtta tttagaaaaa aatgatccta ggcttactga
    27541 cggaagtccc aaattttatt tttatgatgt aggtgccatc gttaaattcg accatcaaca
    27601 atattacttg actcattatc cgatgttgct cgggcctaac gacactatcc gcaatcttca
    27661 tggacatatc catcattaca gcgtacctat cgagaacgat gtcaatgtag gcgtagacgc
    27721 accggaaaga gaactcttaa aggaaaaact accatttggc actccgttat ctgagcaaca
    27781 aatcaaagaa atctatgaag caaagaggaa gtacgatagg tcgtgaaaaa actgccctat
    27841 atcaagtatt aaacaaataa agataattac tcatattttt catttgagta ggcttattgc
    27901 cacaggaaca ttaggatcag aaatggagtt gtaacaagac ttctgtcaca actccattcc
    27961 atactattcg tctttttgct catggtgttc ttttaccagt tttttctgta attctttatc
    28021 ataatttcgg gcagctgacg ttaaaaataa aacgattccg cataataaaa tcccgattcc
    28081 ggaaaacaag aacatttttt ctacccctaa acgatcagct aatggtcctg caaataataa
    28141 tccaattgga cctgccaggt tcatcaaaga gttgaagttc cctaagacac gacctaaaac
    28201 attcgattca taactctgct ggatcattgc catcagcagt gtattgaaat atggcgttgc
    28261 acaacctgct cctgcattca agataagaaa gtacaagaac ccttgactat cgtttggaac
    28321 caatccgctt gcaccgatcg tcaggcccat aatgatgtag gctataataa taggtttcat
    28381 tcggtctttc catgttccta taaaactgat cagtgcaccg cctaagagca ttccgactgc
    28441 gtagactact tccactgctc ccgcctgtcc aacggaacca ccaaagtaat ctagcgtcat
    28501 caatggatac agactgattg ctggcatgaa caacagcatc acaaatgcac cattgatcgt
    28561 tatgtaccat aaccctttat tatccatcaa ttgctgcagc ccaaattttg catctttaaa
    28621 aatagtaagc ggaacttcca atatttcagg agaaactttt gggatcttca caaaaatcaa
    28681 taagccgaca ccaaaaactg cccccaatac atctaataaa ataaggtaat tgacaggaac
    28741 gactgaaaac aaagccgcac ctagagctgg cgcaatgatg aaattcgctg attggaccat
    28801 tcctaactgt ccattcgtcc ttgttatgtg agaagatgga acgattgtcg gcataatcga
    28861 ctggatcgtt ggcatttgga atgtttgtgc gaccgaacgc ataaacaaag acacaaaaac
    28921 tagccaaatc gggaaggatt ctgaaatcgt tccgactact gctaaaataa gcgcgaaaat
    28981 cgctacaata atatccgtga caatcaaaag agctttttta tcccagcgat ccactagcgg
    29041 tcctacaaat gggcttagta aaaccatggg aatcattcct agtaaagtag caaaactcaa
    29101 aattgtcgcc gaaccggttt ctcttgtcaa ataccagatg atcgcatatt ggacgaccat
    29161 actagtgatt cctgacaaaa actgtcccga taaaaagaac aaataatttc tcttccaatt
    29221 cgttaaaacg accgactgtt cattttccat aaacatccct cttttctttt tcattttgta
    29281 tagcgtttgc acaacaatca ttatacggga ttcttttttc aaaaaatacc tttttcccta
    29341 aataacaatc gtttcaattt aaatttcttt acttagaaac aataaatcag caaaatatta
    29401 aaaataacaa catgtgttaa atttaaaaat aaaaaagaca actaatttgc gtcgtcctta
    29461 caaagttagt catctaacaa taggccgttt caaaactagt tgtcttttta tatccacaga
    29521 tcattcgact ttttcgaata acccacggct aatcatccca tccatcagaa aataaaattt
    29581 atcttggctc attttctgtt ttggatcttt gaaaatattg actgctttca atccatttgc
    29641 tgtagtgatc gacattttca atgtttgaat atcttttgat acagtgattt ttaatttgaa
    29701 tccttctttg ctttgcggcg tggcatctaa tggtcggatc aattgataat tgcctccgtt
    29761 tgtttctgta aatccattat cgcgcaatgt atatcttttt gcatcaggat gcaaacgata
    29821 gctaattggt gcgcccagta ccgttgctgt tgtttcaaat gccatgattt tcacctcgat
    29881 aaaattagtt tctctttatt ataaaagttt atcctgattt ttactaggtt tttcagttaa
    29941 gttccctaaa aaatgaaggc gttctctcag acatctgttc atataagaat gacttcacta
    30001 gttatttttt gacagcacga actaattgat caaggaaata ctggatgtcg tcttcggtat
    30061 ttcccaaacc aaagctgata cggatggatt cttttagtat cggtgtatct tctccataca
    30121 ttgcttctaa tacgtgtgaa ggctcgatat ttcctgctgt acaagctgaa ccagtagaaa
    30181 tagcgacccc attaagatca agcttcatca gtagcaaatc atttgcttcg ccaattaagc
    30241 gaagattcaa cacatgaggg agtttattcg ttaaatcacc atttacttga taagggattt
    30301 cgtttgcttc taatgtatca agaatcaatt gctggaatcc tttatatttg ttgttacgtg
    30361 cttcacgttc ttcaggtgac agcagttcaa ctgcttttgc cataccccag atccctgcta
    30421 aattttctgt acctgcacgc cgcttttctt cctgctcccc gcctaaaagc aatgcaggga
    30481 tatttactcc gtctcgttta tataagaaac cgacgccttt tggcccattg attttatgag
    30541 cagagatact taataaatcg attcctaatt cttctggatg gatcgtttgg tttccgtacg
    30601 cttgtaccgc atctgtatgg aaataagctg gatgatccgc taaaagttca ccgatttctt
    30661 tgattggcat caaatttccg atttcattgt tcccgtacat cacagagacc aagatcgtat
    30721 cttctcgtaa cgcttttttg acctcttcga tcgacagatt ccctttttca tctacaggca
    30781 gatatgtaac gtcaaacccc tttgtctcta aatatttcat ggtattaaga acagcaggat
    30841 gttcgatagc tgtagtgatt agatgtctgc cttctttttg acgcgagaac gctgtttcta
    30901 agatagctgt attatcccct tctgttcccc cactgttaaa gatgatctcg tgaggttttg
    30961 cttgcagact gtctgcaatg atttgtcttg ctgtttccaa tttttcatgc gcgtggcgac
    31021 caaatccatg tatgcttgaa ggatttccaa acgtgtcttg catgattgcc gtcatcgctt
    31081 caattacttg tggatgcatg ggggtagttg ccgcatgatc cagataaata gttttcactt
    31141 cattcatccc ttccaaatta ttgctaggca cattctagca taaaatagcg aacaaaccaa
    31201 agaagagctc atttttgtct agaggacgtt tcatgggcat agaaaaaagg agctgtgaca
    31261 aaagtcttgt cgcagctcct tgaagctgct cacttctgtc acaacttctt tccctctttt
    31321 ttttgtttat tcgaattctt tttcttccag acggaataac ggactcatcg gtgtattttc
    31381 atggatgcgt ttgaccgctt cacccattaa ttctgaacat gtaacgattg ttaagttttc
    31441 tggatgacga ttttcttctg tgaaaacaga atcagtgata caaatatctt tgattggtgc
    31501 attgtctaaa atacttttcg catgatcgga caataatcca tgtgacgcac aggcatatac
    31561 ttcttttgcg ccgttttctt ttaatacatt tgctgctcgt gcaagcgttg ctcctgtgtt
    31621 caagatatca tctaccatga tacatgtctt tccttgaaca tcaccgatca cataaccacc
    31681 ctcagttgaa gcatcatctg catgatctac gatggctaat gtgctgttca aatattctga
    31741 aagactacgt gcacgctgaa caccgctgtt ttttggcgaa acaactacga tgtcatctcc
    31801 cgtcagacct aattgacggt aataatgagc aaacaacggc atcgtaaata aattatctac
    31861 aggaatatca aagaatcctt gtacttgaac cgtgtgcaga tctaaagtca gtactcgagt
    31921 tgcccctgct tcttcgatca agttagcgat caatttcgca gtgatcggtt catgaggttt
    31981 cgccgtgcgg tcttgcctag catacccata gtaaggcaat acgacattga tcgttttcgc
    32041 acttgctcgc ttcattgcat cgatcatgat cagtagttcc atatagtaat cattgacagg
    32101 tgcattcgtt gattgaacga tatacacatg atcccctcga atactttctt cgatattgat
    32161 ggaaatctcg ccatcactaa attgcttcac gacgctttta cctaattctg ttccgaacac
    32221 tttcgcgatt ttttcagcta atggacggtt gccattcaaa ctaaaaatct tcaacgtgtc
    32281 atcctgatac ttattactca tgtagtcctc cccaaaactt ctctattctt cgatagttta
    32341 acatattttt ccaagatttt ctgttaattt cagcaaattc ttaaggaagt gaaactgttc
    32401 cgttactcca actggcagga atgactttat gataatcgcc ccagttgtcg tgtactttgt
    32461 aataacgttc tcctgtttca gtttcaaaat aagcgtacgc tgtcacccaa tgattgccat
    32521 atgtactgcc aagtactttc aaaataccaa tcatcacagg tttgccttca cgtatacgtt
    32581 ttgtagctcg ctgccatgaa cctagaagag ttgctcgtgc acgctcatga ttgccattct
    32641 tctttaaaaa actagaaatc cctgtactaa cctgaaaagg aatcgtagga agtcctagtg
    32701 gttggatctg gctacgcaat gccttcgtca aaaccaacga atcagccgta tttttcttgc
    32761 gaatctcatt tggaagcatc caccccttac gatagtcttg ataataggca aggagaactg
    32821 ctgccgcgta agtaccacaa agatagcctg agggggtagc ccatttctta tacctcaaaa
    32881 aacgttcttc tggcaatccc acccagcaat ttgcgagttc ctttcctgac cttttttctt
    32941 cgatcaaaac gggaatatct tcttttctgt tgatccatct agataactcg attggctctt
    33001 cattgatcag cttccatttt ttacaagaaa gaccctcttg ataagtatat tttgctggag
    33061 aatattctac cggatgtcct gattgatcga taatcacata actttgatct tcaaaaacca
    33121 gagctaaaaa aagcaattta ctttttcttg actgcagctg ctgagtgctg gctaattttt
    33181 taattttttt atctactttg aaatataaag gtttatccac tacttgattc agttgggatc
    33241 gcctcctttt tttcattata agtgaaggat tgaaaaaaac aaatcagttt gattgttcga
    33301 attttcaatt ttttatgttc tatttgttaa ttccttcatt ttgtaagata atgggcacac
    33361 gaaacaaaaa aggagcgatc gatgaatgaa caaagaacga ttgaaacaag atttctatga
    33421 agcagtcaat gaagaatggc aaaaaacagc aaaaatccct gcagataaac cagctacagg
    33481 cggtttccaa gatttagttg acgggatcga ccaacttttg atgggagaaa cagacgatct
    33541 gcttgcccat ccagaaaaag tgtcttcaga aaaaatgaaa catttccttg catattatca
    33601 actggcaaac gattatgacc gaagaaatga gttgcatgct caaccgcttc aacctataat
    33661 cgaaaaagtt gaatcattgc agtctttcga cgaattgaat aagcaatttc cagaatggct
    33721 gttggataca ttaccattgc catttaatct ggatgtagat gcagatatga agaatgcgca
    33781 aacgaacgcc ttctttgctt atccgccttc tttattcctt ccagataaga cgtattatgc
    33841 atcagaccat cccaacggtg cacaactaat aggcgttttc tttgatatgg ctgttcagtt
    33901 attagagctt gccggcaaag aaaaaagtca ggcagaagca atcacggaac aagcagtgca
    33961 atttgataaa ttgattgctc ctcatgtaaa aagtgcagaa gaaaacgcag attacagcaa
    34021 aatgtataat ccgcaatctt ttgatacatt tattaaatac acagataaac tggacttgaa
    34081 aaaacttgtc acgggtttga ttgggacaac tccagataaa gtgatcgtaa ctgatccagt
    34141 gtacttcgat catttgaatg acatcctgac agaagctcat tttccgttat tcaaaagctg
    34201 gatgatcatt aaaacagtac gttctcttag tggttatcta tcagaagaat tccgccaagt
    34261 cagcggaatc tattctcgcg tattgtctgg aacagacgaa gcaatgcctc agaaaaaagc
    34321 agcgtattac cttgcctctg gacaattcga tcaagtagtt ggcgattact acggaaaaaa
    34381 atattttggc gaagctgcta aaaaagacgt agaacaaatg gtgaaaaaaa tgatcgccgt
    34441 ttataaaaaa cgtctagaag aaaatgactg gcttggggat gcaacaaaag aaaaagccgt
    34501 cataaaatta aataacttag gtatccaagt aggctacccc gataagatcc cagcattatt
    34561 cgaccagttc aaaacagtac ctgcagatca gggtggtact ttgctttcaa atactcttgt
    34621 tttcagcaaa ctcacattaa aagatcgttt cagcaaatgg aacaaaccag tggatcgttc
    34681 agaatgggaa atgagtgctg acacggtcaa tgcttattat catccatttc ggaatgtgat
    34741 cgtctttccg gcagcgatcc ttcaagcacc attttacagt ctagaacaat caagctctgc
    34801 taattatggt ggaatcggag ctgttatcgc tcatgagatt tcccatgcat ttgataataa
    34861 cggcgcttta tttgacgaat atggcaactt gaacaactgg tggacagaag aagatcttac
    34921 acaatttcaa aacaaagcac aagcaatgat cgaagaattc gatggtctgc cttttgctga
    34981 cggaaaagta aacggaaaat taaccgtttc ggaaaatatt gctgatgcag gcggtctaag
    35041 ctgtgcattg gaagcagcaa aaacggaatc agatctttct atcgaggaat tctttgtcaa
    35101 ttgggcaacg atttggcgta caaaagcaaa aaaagaatac caacaacttt tactgcaaat
    35161 cgatgtccat gcccctgcta aactccgagc aaacgtgcaa ccaaaaaacc ttgacgaatt
    35221 ctatgaaaca tttggtatca cggaacaaga cgctatgtat ctagcgccag aaaaaagagt
    35281 gcgaatctgg taagaggttg tgccagaagt ggtcagcttc aagaaataag aaggaatttg
    35341 ggaaaatcgc ttctcaaatt tttgtgacat ttcggcttat ttttcgaaga agttacttct
    35401 ggaataccat gtattagagg ttgtgaaaaa gctgcttagt ttcaagtagt aagaaaaatt
    35461 ttgtgaaaat ggctttccac atttttgcat aaatttgact tactaccgag aaactggctt
    35521 ttgaacaccg tttatcgaga ggttgtgaaa tcagcgtcct gacctgagta ttagagaaaa
    35581 aaacggaaaa atagttcctc atattttgcc gtttgttgag ctaataccga gggtcagctg
    35641 attgaacacc gtttagtgga gagattgtga aaaagctgtc ctgcatcaag taataagaac
    35701 agccaatcaa aagcagcaag gagaacctca tctataaaaa gatagaagga agtgagacat
    35761 ctattggtct catttccttc tatttttact ttcctcagtc aattgtttta catcataaaa
    35821 ggagaaattc cacttatttt tttcgctgtt tcatgacata aacaactaca ccgattaaca
    35881 aaattcctcc agctaatgct agcaacgggt tattaaattc tcccgttttt gggagtcctt
    35941 gagtgccatt tttttcactt tcttttgctg tcctatccgg atgagaaggt tttccatttg
    36001 gttttccatt tggtgattca tttcctggat tttctctttc tggtttgtca tttcccgggg
    36061 gcacatcggc agtttctcgc acagtgatcg ctttttgctg ggtaacagat tctgtatgag
    36121 cagaccattt ttccccatca aatatttccg aacgataagt gacttctagc gtatattctc
    36181 cttttgaagg tgctgaaaaa atagcggtat gattagaaaa ttgagcactt gactgtgttt
    36241 caacgatttg gatcttttct gggaaataac gcatatcttc aaagacaggc gttccttgat
    36301 ttggtgtgaa agttgacaca aaaactgtaa aaggcttcag ttcaatgata tttccttctg
    36361 cattctccgc cacaccattt gcttcttgta taataatcgg attattttgt tcatttggtt
    36421 ttgtctgatt tggttcttgt acgattgcat actctggata ttccgaagac ggtgaggttt
    36481 ctccgtcaaa aatagcacct acagataatg gaagaccatc cactctttcc ttttctgttt
    36541 gacgattccc cggtttaatc tgcatcagtc catgaacatg gatacttcca ttttcatagc
    36601 gcgtaacagg ctgctgacct tctttcgaac atgtatttag agactcgatc caatcttttg
    36661 atatggtatg tccaagagta ttttctgatt gtttctctcg ttcattccaa tagtagttca
    36721 acgagtggta gatttgatct tcgttttgat tcaatgggcg gatctgctct ctcatctcaa
    36781 gattctctgt tcggaaagat aagataccag aagcatggta gtctgtgttt cctgctgaag
    36841 aggtataaag agccacatta tatgaaccgt tattaaaact tgtactgcta ttgacgataa
    36901 tatctgttcc gctattggaa tcgattcctt tcgcaagatt ttcgaaagac aaactattga
    36961 tcaattcatg agggcccgtt aaagaagaac cgcccatttt gaatccatta ccgttacctt
    37021 ctccctcaat tcctggtacc cagccgtttc gataagcaac tgagtttttg atgattactt
    37081 ttccgatcga acctgtttca tttttagcaa aaaggtccca accgtcatct gcattatgat
    37141 acgcgataca accgtcaaat acatttcctt ctcctattgt cagtttggcc gcaaatccat
    37201 cggcatcttc aaatcctgga tcggcatttt caaaagatgt acagttcttg actaagttat
    37261 gcgcgggcca tgcttcgaaa ggatcatttg agctaccgct gatttggatt cctgtatttc
    37321 cattccgata agcatgaatc tcttcgatca aattatgatt tccagaaatc tgtaatcctt
    37381 tttgcatagc tgcactattt gtaatgtcaa accctcgtaa accccagtag tgactgaaca
    37441 gtgtcatccc atttccagtt cctttaaaat caagtacagg acgcttgttt tctggatttt
    37501 tctctgcaat caactgaatc gggttttctc ttgtcccgtt aacgcctttt ggaatcgtca
    37561 atcctctttc gaaggaataa gttccaggcg ccatgacgat tgtctgccca gcatatgcgt
    37621 atctcaatgc ttgtgtcaga gataatggat tttgtctgct tccgtttcct gcatctgacc
    37681 cttctggtgt tacataaact gtcgaatatt taggtttacg gtagtccact gtatgaagaa
    37741 tctcttttgt ttcataacta gtcatctcta cgtattcttc tggcggatag tttttatctg
    37801 gcgtaaaact gaaagtaaat tcgttcgttc cttccatcag ttttgtctga taatcaaatt
    37861 cccgattcgc agtgatagcc aagttttttg cgttcttaag cacctctcct gttgctttat
    37921 ttttaattgt caataatccg tcagcatttg ctctaaagga catagtatga tcttggtttc
    37981 ctgaatgatt actagaggtc acagtgtaaa tcggatcaat caagcgagta ggtctttctt
    38041 gcccaggagg atctgtcgca ggatctgagt aagcaacttc gattccttct accttgatcg
    38101 tcatattcct tgctgcagca aatcctgcat aaataaccga ttcatctgaa gcgtataatt
    38161 tttcccagtc atacatgatg tcagaagcca tttcttctcc tgtttcagga tcgtaataat
    38221 atgcctctaa accagtattc gtttttttca tctctacgtc cagtttagta atcgagttcg
    38281 tggccggaaa cagattccca tgaccgtttc cgaatatatt ataagaacct gtttcttttt
    38341 ccaagaaccc tgctgaagtc tctaaaggtg ttgtttccac tgagacactc ccaggagctg
    38401 gcgcagcttg cggatcttca gatgatattc ctgtgattgc ccgtgtcccc aatccaagtc
    38461 gcatcgtata tttgctgccg ctcgtgttga ccacttcttg tctatccgag tcccagacat
    38521 attcgatccg actgcctagt atggcaaatg aatttgaata aaatgcctgc ccaaacaaat
    38581 gattttttgg tactgcgtct cttaccatta aagcaaatcc ttcttgggca ttcgtatagg
    38641 tccaagattc tatttcgatc gtcgcccgca tcctaaagtt gatgtctttg gggattgctt
    38701 gataataata ggtcattcca tctgatccgc ttgactggaa ttttccgccg ttattcgtgg
    38761 aagagagccg cacgccgtct tctaatggtt caatggcata cctactagca cttgtgcttt
    38821 ctccgaaaac aacccctttc cactcttcat ttgcttcttc agattgcttt tttgtctttt
    38881 cattttctga ttctttcatc ataggatcat ttccttcttc cgtatccata tcttcactag
    38941 ttcttggtat ttcctccatt gttgacatcc cttcaatgtt cacgtgtgca ttttcttcgt
    39001 tcacagactg ttgttcgtta gcagcaactt caacgacaga aattaaaacc ggggtactga
    39061 gcataaggag attcatcatt agcgcactgt attttatacg tttttttaac aagtgtattc
    39121 ctcccgtaac aaattagaaa gcgtttactt aaagtattac ctaatttatt tttactgtca
    39181 ataaaaaaca aaattataat aagtgtattt tataaaaaga aaaaatataa cttaagttgt
    39241 tgataataaa gagatttatt aacgtaaaaa gaataagttt ttttaaaata acgtataaaa
    39301 aagagcataa aacaaagtcg tttgttttat gctcttcttt tattattttg tgctttttat
    39361 tttatcaaat cactttgttt ttttataaaa tttctgcgcc tagtgtggac tcaaaatgtc
    39421 tcaatgccca ttcatgtcca acgggatcga aacttgccac ggaatctttg aaaatatgga
    39481 gtttgtatcc tagattatac gcatcgactg ctgtatgcaa cacacagata tctgtacata
    39541 cgccggtcaa ataaatatct gtgatctggc gttctcgcaa acggatgtct agatctgttc
    39601 cactaaaagc ggaataatgc cgtttgtcta tccaataaac attttcctct tgctcatagt
    39661 cttggtataa actttttaaa gagccgtaca attcccgtcc acccgttcct atcacattat
    39721 gcggaggaaa taagcggttc tccggatgaa aggaatcttt tggatcatgc gcatcaatgg
    39781 caaaaacgac aaaatccttt tgatcaataa acttctttgt atgagcagtc agcttttttt
    39841 caattacttg tcctgctgtc ccagtagtca gctttccatc ctctgcaaca aaatcatatg
    39901 tgtagtcaat cgagatcaat gcgttcatct tagtcctcct tatacgagat gtttgacaaa
    39961 ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg ataaattcgt caaagctttg
    40021 tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg atcaaaaatg ggatattgaa
    40081 ctgacgagct gtttgtccaa cagcggctcc ttccatttcg caagctaatg cttcagggaa
    40141 attcgttaag atttctttga ttttatctgg cgagtcgaca aatgtatcac ctgtaacgat
    40201 caatccttct tttgcgttca aattcgttgc ttttgctgct ttaaccattt cactgcgcaa
    40261 atatgtgctt gcttcataat atagaggcat tcctggtaat tgtcctgggt tgtagccaaa
    40321 tcctgttgca tctgcatcaa aataagctac tttgtctgat ataacgatgt cacctacttg
    40381 taagccttca ccgattccgc cagctgaacc tgtattgatc accatgttca cgccgtattg
    40441 ttggatcaac agacttgtgg taatagatgc taagactttt cctataccgg aacgtacgac
    40501 gatcacttcg tggttaccta atgaacctga aataaataaa gcgcctgcgc gttcccatga
    40561 taatggttcg cttaggttct ctctcaaaat ttttacttct tcttccattg cgccgataat
    40621 accgattttc atctttttaa ttccttcttt ctatataaat gcgattgcta aaaaaaccac
    40681 acataacatt aggataacaa taataagaga tttcatcaaa aaagagttcc atttggtcgt
    40741 cttttcccgt tcgctgatcc ttgttttcgt gattggttta ttctttttgc tttctttacg
    40801 ataaaagctg gcaatctttt tttcttcttg ctgataagct gtttctgctt ttcgctgttt
    40861 ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga agttcgcttc gcgtaataag
    40921 cggtcctttg gccataagcc gatccctcta ctttcctttg gtcaatagtt cccagtattg
    40981 aatcgcataa atcgtctttg catcacaaat caactgatct ttcatcgctt gttgcgcctc
    41041 ttcaagagtt agatgataca actccagtac ttcgtcttca tcttgtgcta aaggattctc
    41101 gaccttctct accccttgtg catgataaat atgaaggact tcatttgcaa aacctggtga
    41161 aagatacatc gaagtaagat ggctcagcga cttcgcacga tagccagttt cttcttctaa
    41221 ttctctagct gctgtcattt cgggattttg tccttctcct ggatcgattt ttccagctgg
    41281 aatctccaaa atcacttttt ctaaaggttt tcgaaattgt ttgacaagaa gcagacgatc
    41341 ttgttcgtca aaagcaatga tcccgacacc gccaggatga aataccagtt ctctcttcga
    41401 agttcctcca tctggcaaac gaacttcgtc tacagcaaca tcaataattt ttccgctata
    41461 gatttctttg cgactgattg ttttttcttc aaattgctca ttgttcagca tatcttctcc
    41521 tgctttcttt gtaatcataa ctgctttttt ttcatttttc aatttttctc aaaaactcct
    41581 tattagttta ccatagataa accttagatt ttctctaaat atagaaaatc cttctttagt
    41641 ccgatactct ttctagtagt ccgtatgcta aaataagtat gtaatcgaat aacgaaaaga
    41701 attcaatact caacgataaa tgacatggag ggtttattat gataaaacga aattggccgt
    41761 ggcttctagc attggtcttg ctatttctct ttgtccgaaa caatagtttt tcttttgttc
    41821 taggactttt attgataggt gtagttttag ttttgtgggg actctttggt tcgcgcagga
    41881 aaaagggaaa acaagaagaa attcctgcac tgactgacga acttgaaact cactatgaaa
    41941 aaagcggtat gacagcgaat gaaatttcat tttttcgaca aacaatgaat caaacaaaaa
    42001 atgagatcaa tcaattgcaa atgaatatgc agcagacagc caaacttaaa gcaatcgatc
    42061 tccgccatga tccagtcaaa gcagcgaaag ctttattcaa agaattggtg aaagaaccaa
    42121 accgactgca tgaagcaagt cagtttcttt atacgcatct gccgaatcta gttgatttga
    42181 caaataagta taatgagatc aatgaccatg agatcaaaaa caaacagacc tatgaaaaac
    42241 tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt aatcgcacaa gattatcaaa
    42301 aattcgttgc tgaggatcta gatgatatcg acgtagagat atctgttgcg aaacagagct
    42361 taaagcgtga taataaatat gaaccagaac aaaaggatta actcttttgt tcataaaaga
    42421 agactcggaa cattcagatg atgtctggct gtttcgagta ctttttctaa agtagagaca
    42481 ttcatgtaag aatcgagaac agattcaaca aggaggaatt ttttatggat cataaaccga
    42541 ctgaaacaaa agatgttaca ccagttaacg atacacttga agatttattg aacaacccgt
    42601 tttcaactcc aacagatacg ctgaccactt cacaacaagc ggagattgat cagctccaag
    42661 agcaacagac agctgctcgt ttaattgaca aactaccggc ggaacgccaa gaacaagcta
    42721 aacagctagc agcaaaaatc gatgctaatg atgcacagtc tgtcatcagc tacggatcgg
    42781 ctgcccaagc aaaattaagt gaattctcac aatcgatgtt gaatcatgtg caagcacaag
    42841 atattggtcc tgtaggagat tccttaactg aactgatgta tcgtctccaa gaagccaatc
    42901 ctgacgaact gcgtgccgga gaagggaaca tcttccagcg tgtcttcggg aaggtcaaac
    42961 aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg tgcacaaatt gacaagattt
    43021 cagtgaaatt agataaagaa aaagacggtc tgctaaaaga taatctgatg ttagaacagc
    43081 tttaccagaa aaacaaagac tactttgatg cactgaatat ttatatcgct gctggagaat
    43141 tgaaaatgga agaacttcaa acaacgatca ttccagaagc gatgaaaaga gcagaagaaa
    43201 ctggcgatca gatggatgtt cagattgcca atgattacac gcaattccta gatcgtctgg
    43261 ataaacgtac gcatgatctt cgcttagcta gacagatcac gatccagcaa gcaccacaaa
    43321 tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa gatccaagca tctatcgcta
    43381 cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt gaccttgctt cgtcaaaaag
    43441 atgcagtaac tgctcaacgc caagtctctg aaacaacaaa cgacttattg aagaaaaatt
    43501 cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga aaatgaacgt ggaatcgtgg
    43561 atatcgagac ccttcaaacc actcaaaatg atttgatcga aacaatccaa gagacattgc
    43621 gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga agtagagctt ggacacatgg
    43681 aagaagatct gaaacaaaaa ctgttggatt taacccaata gatcaccttt cgcttgttac
    43741 taaaacaaac gcccaacaac agtttattac aactgttgat gggcgttttt cttatctttc
    43801 ttctaacttc tccattggag tctatttctc attcatcttg ctgtgtccat agccatagcc
    43861 aaaataaatg atcagtccaa taaccattac gataccgaac acgatccatg tcacagtttg
    43921 cagacgaagc atcaatacaa tacaagtcaa aatggagacg attggcaata gcggaacgaa
    43981 tggcactttg aattctccag cttttggttt gccaaacatt tttctcaact tcagtaagcc
    44041 tagactaaca agcatcaaat aggcgagcgc catgatattc gttagttccg ctaatacatt
    44101 caatgggaaa aatccggcaa agaatacagc tgccacacca gcggcataag tcgctttttt
    44161 cggtgtacga tgcttttgat cgatttctgt cataaactta ggaagtaagc catccttgct
    44221 gacagcaaac agtaaacgag caagtgaata catcatcgag atcgttaccg tcagtagcgt
    44281 taaaattgcc cctacagcga tcaccgcgcc cactgttcca tgtcctacat acctcattgc
    44341 aaaagccact gggtcattga ctcctagttc agtatacgga acgattcctg tcaagatcag
    44401 cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa ccaatgattc ctcttggcac
    44461 atctttttgc gggtttttga cttcctcggc tgccattgca acggcatcaa atcccaagaa
    44521 agcaaaaaac acaagagctg caccatctag cacaccggaa aaaccaaaag gcatgaatgg
    44581 ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg ataaacaacg cgatgatacc
    44641 aaatttgaca tataccatgg cattattcaa acgtaatgct ttcttggctt caagcgacac
    44701 gatgtaagtg acgaatatca ctacgagcac cgcaatcaag tcaatgtatg taccattttc
    44761 cggattgtac ccgccagtta atgcttgtgg aagttcggcc cctaaacttt tcaaaaaacc
    44821 ttgcatatag ccagaccatc cggaagcaac agaggaaacc gctagtaaaa attccccgat
    44881 caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat acaacataca gataggcata
    44941 tggcccacca atcactggca ctcgcgaagc aaattctgcg aaacttagtc cagacaaaat
    45001 gacaacgatt gcagctaaga caaatgaaag tgacagtgct ggaccagcat tttgattggc
    45061 cgcaacccct gtcaccacaa atattcccgt accgatgatc gcaccaattc caagcatgat
    45121 caaatcagac gtttttagtt cttttttcat ctcactcgta tgattgagat tgatctcttt
    45181 cttacgaaac aaatccattg aagttcctcc ccattttagg ttggaaaaca gcgattaaac
    45241 tgttttcata taccctaaaa ggataccacc taaaatgact aaaaggcaac caacaataac
    45301 ataaatgagc tcttttttcg tttttctttc gccaaggatg aaaatcccac ctaaagtaga
    45361 gataatgatc cccatttgtg aaagtgagaa gccaacagct aaaccaagtg atttgactgt
    45421 tagaagcatg cagatgtttc ctaagcccca taataatcca cagatcatgt ttttccacac
    45481 aaactgatcg actttaactt tacggaaagc aaagaagctt gcgcctaaga tcattccgat
    45541 actttgaggt aagataattg acatcgcatc caaattcgcc cagttgacga tgatggtata
    45601 tacaccataa ccaatcgtag agaagatcaa tgctctaaaa cccttaccaa aatcaagcat
    45661 tttattatct gtatctattt ttccttcgtc atcttgtcgg gcagtcaaat acgcaccgct
    45721 tacaagaaga gctaaagcaa taataccgta tacataatct ttcgtttgtg tccattcatg
    45781 gaagaaaacg gctcccgcaa tcgtattcaa aattaattga aaaccagtag acaatggtag
    45841 ccctacagaa acacccatgt acttcattcc atggaactgt ccattttgtc caacactcca
    45901 agctaagccg gataaaaatc cgatcaagaa aatccaaggg gtgattacag gactaacaac
    45961 gaaaaagaca actaaagaaa acagaaatgc accgatcgtc attcctagtg tttgttgatt
    46021 ggcatcgcca ccaattttac cacttactaa accaatacta ccccatgcca acatagggac
    46081 taatgctatt aaaatttcca taaccaatct ccttttattt cacatgataa gaaacttatc
    46141 agcgaaaacg ttaacaacac aagcactttt tttcgtttct aacgattttc taacgttttt
    46201 cattataaaa atttaagatt gggaaaaata aagtcattgt tgtccctttt ccttcagttg
    46261 attcagccgt caaatggata cctaattttt cggccagatt ttttgctaaa taaagtccta
    46321 atcctgttga atgtgttttg ctaagtcgac catttctccc agtaaatccc ttatcaaaga
    46381 tccgtttcaa gtcttccgct gggataccga tccctgaatc tttaactgaa aggctgactc
    46441 cttgcgcttc cttttctatg ctcacaatga tgtttccgtg atccggcgta tatttgatgg
    46501 cattactaag caattggttg aagataaaag cgatccattt agcatcggtc aaaacagtat
    46561 gatcctcacc aacgatctcg tagcgaatat ttttttggat aaaataattt gcctgactgc
    46621 gtatcacagg ctgaatgatt ccttttagtc catattcttg gatgaggtag tcccgcgaga
    46681 aactgtcgag tcttgcataa tacaaaactt gttctacgta ttcatcgatt tttgataatt
    46741 cattttctag taatgtatac ttttgatcgt caatatcgaa ctcgatcgaa cgaactagca
    46801 atttagaagc ggaaagtggc actttgattt catggaccca tgagtcaata taatcttttt
    46861 ggtcttgttg attactgatt gctgattgca ttacttcttg gtgttcacgg atcagctgat
    46921 tgatatattc ttctaccagt ttttcttctt ctgtcctccc accactcaaa taattttgca
    46981 aaggggaagt tttttgtatg tcggctaatt ttccccacca tcttctcttg ctcaagtaac
    47041 cacccagcag ataaaaaatc agaaagacgc cctcaatcaa agctaaataa gcaattgtgc
    47101 tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa gatcgtcaat gcaataaaaa
    47161 acagccatcc caagatcaat gtccactgat ctttcaaata tttaaaaaaa ttcatcttaa
    47221 ccgctcatcc tttcagggaa cgatatatcc ctgtcctact tttgtttcga tatatccttg
    47281 gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg ttgactgtca atgtattgtc
    47341 atctacaaat cgttcgtcat cccacagtgc tcgcagcaat ttttcccgac ttaagatctt
    47401 tccattgtgc ttcatcaaaa taaataatag acgatattca tttttgctca aatcaatgat
    47461 ctctccgttg atttccatac ttccgttgtc aacattcaac gtaatgccat tgtggctcat
    47521 catttcacta gataattctg tgtagttgta agaacggcga agtaatgcat tgatttttgc
    47581 aatcagtaca tcgatttgga aatgttttgt cacaaaatca tctgccccca tgttcatagc
    47641 catgatcata tccatattcg tattcctgct ggagataaaa atgatcggta ctttcgatac
    47701 ttcacgtatt ttttgacacc aataatagcc atccaaaacg ggaagattaa tatctagtaa
    47761 tacaagctgt ggttcttctc tttcaaagtc tgagaacact tgattgaaat cggtcgttcc
    47821 aaaggtttca aattgccatt tctcaagttc ctccatgatc aattgtcgaa tgatctcttc
    47881 gtcttcgact accatgattt ttgccataaa aaatccctcc atctttcttc ttgctactac
    47941 aatatcaaag aagcccttgt gaaacaagtt ggcttacctg gaaaatgtca ttttagcaaa
    48001 cgttaaaaaa aactggaaat atgcgattta atcgcgtttg tctcttccaa gcagcttaaa
    48061 tttattatgt tattcttaat aattggtcta ttatctgtta tttttctttc gttgtttttg
    48121 tcttttaaat tttcgataac accaataacc gaaaagcgtt agcactagca atatacctag
    48181 aaggagtata agtaatcgat aaagatggta tttttttgtc tgttctatct ttgaagagac
    48241 ttcttctgta aagggaactc gtcttcctgt cactagtaat cggtgagtgt tcacggtgta
    48301 aggtgtacaa gtgataagtg tgactagatc ctgcttttct ctaattgcta atgtatttgt
    48361 ttttgttgga aggacaacaa ttttctcaac gacttcataa gcgagcatcc gaccatacac
    48421 ttcaagatag aatcggtcat cttgctccat ttcttcaaga tccgtaaaga gttttttctc
    48481 agcaataccg ctatgggcgg taatcacgga atgtgtactc tctcctccag ttggaaaaga
    48541 ggtgccttgc aggactgtcg ctcctctatc taacaaaaga tcattcgttt catcaaataa
    48601 aggcaagcta acattgattt tcggaatata gatggctccg atcatatgtt gttcgtaata
    48661 ttcttttcct ggattttgga catttctaac cgcttgctca aaaggatctt tgacttgtcc
    48721 cattcctggg attactgatg tatgagcaag cgtctgattt ttcttttcat gcactgctaa
    48781 ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc ttttgataat tctcgatcct
    48841 tctttgatcc aaataattgc tgagagcatc tgctaaaaat ggatatgtaa aaactgcagc
    48901 tccggcaaga aacatcactg ccataaataa ttgtaataaa agactgattt gggtttgagg
    48961 tctattacct attttctttt tcattttctt caccttctta tttaaaaaaa ctgcagcaac
    49021 ctttataaaa ccgattgctg cagcttattt ttgttgctta tacttcagac ttcattttag
    49081 atttacggta ccagctgtac gctccaatca tcaaacttat tccgattagt aagaatgcca
    49141 agagcccgtt cccaccagta gcaggaagtg acccgcgttt catattcggt actctttctg
    49201 gccctggatt ttctactcct acgatctgaa tttcttcatt atagcttcca taagagatga
    49261 taaattcagt aaaggctgcc tcacctgttg gaagtacata accttctggc gccatggtct
    49321 ctttcatttg ataggttcca taatctaacc cttgaacctc aaagtatcca tttccatctg
    49381 cgacaaattc tgttgcttgt ccgcttgttg cccactcaat tctatctgct cctgattcat
    49441 aaacagctaa aggcgactcc cctttataaa atattgcgta ttcagaacgg tcttcattcc
    49501 acaattcgaa tcttgcatct ttaagcaatt cactgcttga tgcatcatat ttttcaaact
    49561 ttcttccacc tgttgtgact ttctctggcg gctctactcc ttcttcataa tcaaattcat
    49621 catttcgtcc gatttcaata accgctgtgt tgttatggaa atcatccgct tctagttctg
    49681 tggaaacctt caaattatac gtgatcgtta aatctcctcc acgtgcatct gctaatctac
    49741 ctaaagtatc tactttggct actccatttt cttctgtcaa aatcgtcacg acaaacccgt
    49801 tgcttactac ttctactata taatcttgtc cttttgttaa aatcgtctcg ccggctcgca
    49861 cttctatttc ctgatcataa taagttagac caactgtagg cgtatctgtg attttgtaat
    49921 ttttgataac agcgcttccg ttttcaagga cagaatcgat gaaataagga ataggtgctg
    49981 tgatcgtgta agaagccgtt tttcctcttt ctagatcaat ataagaaatc gtttcttgtt
    50041 ctccagcttc attaataatc gttacttgtc taaggtcgct ttcctctaca ttcatctgtt
    50101 tatctgcttc tctgatttcg tttttcggat acaaatgcac atctgtattg tatgtgtctt
    50161 tccatgtaga accatcccac acgccatcca ccatatcagg catcatcact ggcatcataa
    50221 caaccattgg agacgcgatt ctggaaatat gagctggcga ataagtttct aagaaaagat
    50281 agactttatc tctagaagaa ggtgcttctt ccgtcacttg gacggtagag aataccgctt
    50341 ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag tcgatatttt aaaaaccaac
    50401 tttctgtgtg ccgtttttga atccattcaa tcgcttcggc agaattaaga ccgtcttctc
    50461 gttccggtgt taaaggatta tctcgaatca aatcgtaata tacttccgtt acgtcaaaca
    50521 aatcaaaatc gactcctgga atattttctt caccaaaatc cgtagaaacc aatccgctat
    50581 tcggtcgttc ttccggaaca gatgagaaac cttttttgtg taatgtaatc gtcacttcac
    50641 ttggtttttg tgcgacttct tcggctttga ctagttttcc gttcccgaaa atccctattc
    50701 ccgcagtaac gagtaacagt acactcattc ccgtgacaat cattttttta ttcatttttt
    50761 tctccccgtt ctttttttat taataaaaca gcacctaaca ataaaagaat tcctagtaaa
    50821 gaaatcaacg ttcttgcttc attcgtttgc gggaatacgg accattttcc tgattgacct
    50881 ggattgcttg gaccactcgg ctctttcggt ttgtccgttg tttcttttac ggtctcgtta
    50941 taaacacgtg gtgttgcgga ttcataggca gagactggaa ctttcccgga ttctaattca
    51001 gccatctcct gtccgttaac tactacatat tgtggttggc cttttgcatc cacccaaaat
    51061 tcagggatga tgacctcgat tctcttactt gcttcatcca tctcgtaacc ttccacacct
    51121 tttaattctt caaaataata ggtgccagaa ggcaagaaac gttgtcctgt cgtaacaagg
    51181 ccctctttat ccgaaatgaa tttgcttaca tttttgtcat tcaaaggatc attgtctgct
    51241 ggtttgatcc attgattttt caagtcatta gcaggagcca tatctaaata atatttcccc
    51301 ccttctatca tctgatatag agcaaaaaca gcaccttcta atggttttcc tttttcagtt
    51361 gttcctcttt gacgtccata cttgaagaag tatggatctc ttagataacc gacattttta
    51421 ggatagatat gaatctcttg cagttcctcg gttgattctg tcgggtgata aattggcaaa
    51481 atagcagcaa ttgggatagc tgtctgatcg atatcaacat ccaatccaac ttctggatca
    51541 atcgattctt cgaaaatcaa ataaactgca tcccgatctt ggtttttctt aggtgctttg
    51601 atacgtgcaa tcccgtctcc taatccgcca accgtatctt tcccagttgt taccgaagtc
    51661 cctatttgga caagcctttc ttctgtgatt agctgacgga tattgtttct gttcgtttca
    51721 gcgatttgct ggacgaattc tttaggagtc atgttttcag cagctttttt ttcatgataa
    51781 taatcggtca tgtcgtaaat ggcaaatgtc gctccattta gtggcaccgt ctgtgaaatg
    51841 atatctgctg tttcccctgc ttcctcagag atagccaagc catcattttc atggtattca
    51901 aattgatcgt tataatcgat atctcgaatc atccgtttat gcaacacgaa ttccacgaca
    51961 tcttcccctg attcttctgc cgacatcgca tgtggaagaa ggactgctgc aagcggtagt
    52021 aaaacaagta attgaagcat caaacacaac agctttttca ttaccttgct ccctcctttt
    52081 tagattgtct gacaaacaaa ccataggctg ctcctgcaag cgccatgaag aatgcagcaa
    52141 gcaggatata gaataatcgt ccggatcctc ctgtagcagg caacggattt tcaggatcgt
    52201 tcccgacact aagctgtaat ctgattcgat ttgtctccaa aagttcatat tctaactctt
    52261 gctgtagatc aggcgtctct gcttcttcgt ttggataata cgaaatctca acttttagtg
    52321 tacccgcttc ttttcctgga actacccgat cattttctat gtcaggttct tctcgataac
    52381 taatttctaa aataaagtaa cctggaagtt ttctatagct gtcaggtgac ttcacttcct
    52441 tgatccgata gaccccttcc aacaagtctg aagcattgaa tagggaagaa tcgtttggat
    52501 ctggtgccaa gcctccctcg atgatttcat actctcctgc actattttgt ttctcaattg
    52561 aaaattctgc gccttcaaga cgtcggctat tcatactgtc aattttcaaa agttccaaat
    52621 cgattggcgc tagttcatct tctaaggtta cttgtagtac accatcgatg atttcatagc
    52681 cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc ttcaatgata aacttacctt
    52741 cttcattcag tgcaaactgg aacacttgat cagatagccg atacccattt ggtgcgacag
    52801 attccgagac ctcatattca ccaatagaca gatgcagagg ttggtcgtaa tttaaatcga
    52861 catacttttc tactcctacg gcacgagaat agaagtatcc gatcccaggc aaacgtttcg
    52921 attcttctgg ttctaacgga ttaccagtgt atgctcgata cagcgggaag gaagaatccc
    52981 ccactttcct gatatcgaaa cctgcaccat ctaatgtatg gccttcatgg tctaattttc
    53041 taactcggat ctcccgataa tcaaacgtat tgtaagcagt gaatgctacc cataaacgat
    53101 cctcttcatc tgttgtatac gtgagatatt gccaattttc ttcacctgtt tgcttgagtt
    53161 ggatagtccc tatagcatta tcgtaaagtc gcaagatcca tgtgtcattt tcatcaggtt
    53221 ctttgaaacc ttctgggttc tggctctcaa tgattttgta ttctgcatag ttggtagttt
    53281 cttcactatc cttataaaca aatgaatctg gctctccgat tggatgaata actttctcac
    53341 catccacgat tacatatttc ccgtcttcat ctactgaata gaacgtgagt ctccctttct
    53401 cttcctctac ttcgttcaca ccagaaagcc ctttcgctac tggatatccg atttccagtc
    53461 cgttaccatc tgtttcataa agttcaaact ctgcgccatt gattggcata ttactgcctt
    53521 caattagttt attgatattc atgtggattg gcttgaattc atttcgcatt tccagtttga
    53581 catacaactc atcatactct gtgccttcta tgttaggctc ttgtacaaca gaaagcttgt
    53641 gatattctcc ttcataatag gaattcggat ctttaccttc cggtccgccg actctcgaac
    53701 cagatgtata ttcatctaca agctcgtagt gacctaacat ctcatcttcg ttggctgcat
    53761 attttttgat ttggaattga tacgtctcat ctactaactg ataacgtggt ggtccagcga
    53821 tctctctgat ccaaaatatg tcgctgactg ctccagtttc atctcctaaa atcggcatct
    53881 tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact tgtttcgatt ggttcggctt
    53941 ccccactctc tggattttgg tcatataatg caaattcgac tccagccaac ggctcgccat
    54001 tttgaccctt cttgctgact tggaaattac ggcctttatg ttcgtaacga atggaagaag
    54061 atgggaaata catccgtcca gtagtgatat cattctcata atcctccagg ttctgaatat
    54121 aggccatatt gttcactgtg caaaaatttc cgtcttgagc tcccgcatca agttttactt
    54181 ggtacttgat cctgaccatt tccccttcac ctaaagaaat cggagaaaat ttataagctt
    54241 ggttttcccg tccgacatac gttgtcggag tcgccactac ttctggtggg gcttgtccat
    54301 gacgttcttc atcccatatt tctggctctt ggtacttcat gccgttttct cctggatcta
    54361 gatggtaaaa ctgaatatct gccgatctgc cagctccaaa tggtgagaca ttaaaaccag
    54421 tttcatcata aagtgtggct tgctggatcg ttctcttgaa ggtagaagag atatcctcca
    54481 aggctgtaac aatgtcattc ttgttgttcg cattgtaata tcgcggtgtt acgccatctt
    54541 cttctaaatc agcaatattt ttcaatacat ttcgtccaat agcatctagt cgttgtctcc
    54601 cgctaacgtt tccatcgatt cctaaaccaa tggagaagat acttggcggg ttctcttgat
    54661 acttgttttt cagccaatga tgataagcaa ctgtgttgat cgcagatgac ttttcttgcg
    54721 aagtaggact gccattcgta ttgttattag aagaaccatc accaacccag tgagtcgctt
    54781 tattatctcc ttcttttacc tctccataac gatagtgtac atttacttca tcttctggac
    54841 ggttaaagtc agatgcatag gtaactggat gagtaaaccc tgcattagaa gtattcccag
    54901 aaaactcctc aaaatttctg aacaaaacgt cattatcttc agcaatctta tcttccatca
    54961 cagtccagtt gctccaagct ccattagtgc tcgtacgata acttgactga atcggtgcat
    55021 aactgaaggt cggtgtccca tctcccacaa cgattaggat tttttctgga tttctatttt
    55081 ctccgccatt gtctttatac aatgtttcat agccattttt aagccctagt gtaagcggag
    55141 ttcctccgat tggtgtacga tttaatgctg tattctcact tcccgtcaaa cgattgatat
    55201 catttgtcaa tggaaagatg tccgaactga tttgctcatg gttattgata gctgtactat
    55261 tatggtaaaa attcaccata ccgattcgaa tatccagact cgggtcagag agcagatctt
    55321 tactcatttc gtttactgcc tcaatcaacg cagcattttt agtttgacta ttggtcccag
    55381 cggttaattc actcatactc gctgacttat ctagaacaaa aacgatatcg attggactga
    55441 tttccttttt ctcgcccccg atgacatcta gatagacatt gtaaagggtt gttgcatctt
    55501 caggatgtag atcagatgct gtgctgcttg gcagaacagg ttctacccat tttttgatgt
    55561 acgccccgtt ataatccaag taactgtttt caaaattccg ttcatttccc caaagctgaa
    55621 cggtttgagc tggaacgttt ctatcttctt cgcctgcagt tgatccatta ttagtagcat
    55681 agtcgtaatt tcgccagttc gctcccccgt ttgtataatc actatcatcg atcaagttct
    55741 ctccgtcata tggcgagcct ttgtctcctt gatagcgttc attgctttgt gtccaatttc
    55801 ctaacacttt tgtatagtca ttcgttgtaa acttcgggta gcgatgctgt tcgttttcaa
    55861 cttctccgct aggatagata tattcatagg ggtcttgcgt agctgttggc ccaagacgct
    55921 ccaaaatacc gttgaataac tggcgttgaa tgcgctggag tgcttgaccg ctgggtacga
    55981 ttggttcgtc cagcttttcc atctcatttt cttcgctacg ttgaatttca gcggaatctt
    56041 ctgtatcagt ttcattttct tgtaattctt ggttatctat tctttcatca accaccactt
    56101 gaagaggtag ttacccttcg ttcacatcgg ggatcttttc tgtttcaaaa atcaatgtgc
    56161 cggtttctgc ttcttttgaa taaacttttc cgacatacca ttcttgacca tcgatcgtct
    56221 cgacttttag gtcttctcca tcttcatcat aggaggatag acctgttcca cgaacgtttt
    56281 ttacggtacc aatcccggcg gctgccgcat cgattgctaa acgtattcta ccttcattgg
    56341 cactttcctt tttttcaaaa gccagatgcc attcaagggt ttcttctttt tcaatatact
    56401 tcccttcaat ttttgcatag tcctcatcta agaatacctc atcctccttt gtttctgttg
    56461 actcagcagc tacatccagg gtcgtcatat gatagactga ctggaaatat ggtccaaata
    56521 gcaaactgac agttactaac tgcaaaagct tcaattttaa ccctttcaca tccttatact
    56581 cctttcaaaa atatttgtct tctttcacaa caatcaacaa aacaatgccc atctaattat
    56641 aatttaattt aattttaatt ttaaatcaaa tgatatcctt tttagttaaa aaacaccaat
    56701 tatacaacat ttattattaa aattaaatta taaaatttta taacgttctc atttaacaaa
    56761 acttaagtta ttaagacaaa tgaataaaca actacgcttt cctcgaaaca aaaaaataaa
    56821 gcacaaatgc gcatttacgc actcatgctt tattcgtaca attaattcag ataacggcag
    56881 cgcataggat gccaataaca cttcctaaaa tagctcctcc taaaatgtca gtaggataat
    56941 gtaaaagtaa aaaaatcctc gaataagcga tcaaacaagc aaataaaaag gctaatattc
    57001 ctgcaactgg acttaattcg aacaacgcag ctactgataa aaaggctagc gctgtatgtc
    57061 ctgaaggaaa agaagaagtc ttcggtttac ttgtgtaaag gacaagccga cgattttttt
    57121 cataaggtct tttccgtctt gtcgtttttt tgataaaaag caaaccaaca aaaaaataga
    57181 tcaaactgcc gataagaatg gctagaccat attctctagt atcaggtatc aagactaaaa
    57241 cagcagcaag gagcaaccaa ataaaaccac cattagagat attactgata aatttcaaaa
    57301 aattgatttt tcgactatcc gttttccgtt gcttggcgaa cttgttgata atactatcat
    57361 cgattttagc aatcaaacgc aacatgttat tcctcctttt taagaacctt ttcttgcgta
    57421 cgatggataa aaagcaggac ttgaaaataa aacaagaaac tgaaacctaa taaagcaaaa
    57481 tagattggtc ctgcaaacca gaatgcgaca agaaggatca acgtgcctgc caaatagaaa
    57541 agcgcgcttt tgaaatcatt aaaaaacaag aaaaatgcag taatccaaga agcttctttt
    57601 tgttccagtt gaagcaagat tttgtaacta aagagaagcg cgcttaatag aagcaaaaac
    57661 aacgtagtat acatcaatgt atagccaatt cccccacccc aatagcggaa aatctgataa
    57721 tcgatcaata gaagaagaaa ataaacactt gctaaactat cttgctttcc ataaagagag
    57781 aagttctcac gaaacttttt ccagaatagt gaaccgagct ttttctcttc attttttttg
    57841 taatgataaa tcatatacag aactgttcga cttgcagttc ctaatgtgaa aacaggcaag
    57901 acgcacagga accatagtag ctgtaaatgg ataagacgtg gaatccattc aatgatttct
    57961 aagaaataat cattggcttt tttaccaatc gatcgattca tcatcctttt gaacctcctg
    58021 tcaaatccat cccttcaatg aaataacgtt gtgcaaaaaa gaacagcaaa atcgtcggga
    58081 tcaagacaag tgttgctcct gccatcagat aattccattg tgtcgttgct tggttctgaa
    58141 aaacttgcaa gccaatctgt aacgtgtatt tttcttgatc ttggatatat aacagaggac
    58201 ctaagaaatc attccaagct gcgttgaagg aattgatcgc aatggtaatc aaggcaggtt
    58261 tcgtcaatgg aagcatcaaa tgactccaaa tatacaaatg attcgctccg tcgatttcag
    58321 cagcttcgat caattcatta ttgatcgata agtaaaattg acgcatcaga aatatattga
    58381 acgcattgcc gaagaacgat ggaacgatca ggggcaaata agtacctacc cagccgatct
    58441 tggagaataa tacatattga gggatcatcg ttacaaaccc cgggatcatc atcgtagaaa
    58501 gaaccaaagc aaacatcagc tttttcccag gaaaatcaag cttcgcaaat ccataagcaa
    58561 taaaagaatt tgaaaggaca ttaccgatca ctactagcac agtgatgaat aatgtatttc
    58621 ttgcatagcg ccaaaaagga aaggcggcaa tcgtatccag atagttttcc catacgattt
    58681 tttctgggaa aaaggtcgga ggataacgaa cgatttcttc cattggtttc aatgaagtaa
    58741 acaccatcca aagcaatgga atgatcaaaa tgattcctat tactgtcatg aggacataac
    58801 ttagtatttt ttttgtttgc cgattcacac tggtcttttt gaaataatcc atcatttgcc
    58861 gtcacctcca gaataataga cccacttagg tgcaagcttc aaattgacaa acgtcagaac
    58921 caacacgatc agaaacagga tcattgacat agccatcgca taacccatat caaaagctac
    58981 gaacgccttg ttccacatat gcagattata aaaaagcaaa gaatttgcag gtccgcctgt
    59041 tccatttttc gtcatgacat aagcttcttg aaaaatttgg aaagaaccga tcgtagaggt
    59101 gatcacatcg taaaaaataa taggagtgat taatggcaaa gtaatacttt tgaactttcc
    59161 ccaaacgcct gctccatcga tttctgctga ttcatataaa gatctaggaa cattttgtaa
    59221 tgtagctaag taaagtagca ttgctcctcc agaactccat attttcatca aaatcaacgc
    59281 aggtttcgtc cattgtggat caaacagcca agctggtcca gggatatgga accagcccag
    59341 aaatgtgttg atcaatcctg cagatggaga aagcagctgc atccataaga tgtagactgc
    59401 tactcctgat aagactgccg gtaaatagaa agctgtacgg aatacactga ttcctttgat
    59461 tttttgattg agtactgtgg ctaaaaacac actgaaaatc gtcgtcagcg gtacattgaa
    59521 gatgacgaaa taaacggtgt tgtataacga tttccaaaat aaatcatcgt gaacgaacat
    59581 tcttttgaaa tttgctagac caatgaaatt catcttggat gtcaaattat aatcggtgaa
    59641 actaccaata actgaaaaaa gcataggccc tagcataaat accaagaacc cgataataaa
    59701 aggcgagata aataaatagg cccatcttgc ttcttttttc tttcggatag ataaacgctt
    59761 caaattttct tgcctcctct gtacctagac agtcaaaaat ggattattga ttttgcttga
    59821 ctaagttttc tactgctttt tgtgcatctg ccaatccttc ttttggtgat tttgttccta
    59881 aaagaatctg gtcaatctgt tcattcacta atgtcgtgta atccggagca tctaaaggaa
    59941 ctggtgtgat caccgtattt ttaaagtttt cgtccgccat ttgatagatc atttttcctt
    60001 tatccgttag gttgtcatta ttcactaaat tctcattggc tgtcttgctc gccatgatat
    60061 caaaactttt ttcgccgaat ttttcttgta cttctggaga agaaagatat ttgatgaatt
    60121 cataagaggc ctcaggatct tttgctccgt atggtacttc caagacgaat ccgccacccc
    60181 acgaccaatg gcctgaacct tcttcttttt ctgggagctg catcacgcca aaatcaaaat
    60241 catccggtgc attttccatc aaactagagt aataattgat attttgggca cgcatggcta
    60301 ctaatccgga aataaatgga tcagatacac cagacccaaa ttcggcttct aatttattga
    60361 tcgtattttt cccataataa tcttgccagt cttggatcca ctccaaggct tccactttat
    60421 ttttagtatc aatcttgact ttttcatctt tgtcaaacca actagtcccg ttatctgcgt
    60481 ttagcgccca tacatcagcc cctaaattcc ataatggata aaaaccgatg cgtgtaaatt
    60541 cttttccttc ttttttatct agtttacgtg ctgtttcttc taattcatcc caagttgtcg
    60601 gtagatcttg ttcactgatt ccagcttctt taaataactt tttattataa aaaatgactt
    60661 gtgtatctgt attgaatggg atcgcataag gatcaccttc atattctact gtatcccaaa
    60721 gctgcggata aaaatcctta gaagtgtctt tttctacata tttggagaga ttagttgctt
    60781 gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg tacaataaca tcgggtgcat
    60841 ttccagctgt tattgcagat aaagatttag tccagatgtc accccatggc tgatacttat
    60901 aatttacatg gatctcgttt tgtgattgat tgaaatcctc aatgatttct tcgatagctt
    60961 cttttcgtgc accggatccc caaaaggacc agaaatcaat attttttctt ccatcacttg
    61021 ttttttcaga agaatttgct gcacttgaac tggcatcgct tgcattactt gatccgcatc
    61081 ctcctaataa aaacaatgct gaacttgcca gcaaccctgt aaacaataat tttttcatat
    61141 tttacctcca aattttaatg taaccgatgc cggttttaat ataacgatat cattttgttc
    61201 tgaaaataac aatttataag gtatgattct aaaaaaatta ttaatgatac caatacttta
    61261 tataattcgc gagcgctttc tttgaatttt ttaatctttc gtagcagaat gagaatgtat
    61321 taaaaataga ttaggaggaa ttttgatgtt tagacaccag aaagaattgc agtttgaagc
    61381 aaaaccagat cgtccgaatc cattaatcgc gaaatatctg caggagctga tcggcggcca
    61441 atatggcgag atgagtgtag ctatgcagta cctgttccaa ggctggagct gccgaggaga
    61501 agaaaaatat aaagatatgc tgatggacat tgcaacagaa gaaatcggcc acgtggaaat
    61561 gttggcgaca atggttgctc gtttattaga gggtgcgcct gttgaagatc aagaagcagc
    61621 gatgcaaaaa gatccaacaa tcggtgccat tatctctgga atgaatccac agcacgcgat
    61681 cgtagctggc ttaggacctc gtcctgctga cagcgtgggc aatccatgga gcggtgccta
    61741 tatcattgcg agtggcaact tgttagcaga ctttagagct aatttaaatg ccgaatcaca
    61801 aggaagactt caagtagcac gtatttacga aatgatcgat gatcgtggcg taaaagatat
    61861 gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat ctatgggaat gtgcagtgaa
    61921 agaattagaa gaaaaagaag gttcaatctt agtcccaagt actttcccgc gtgacaaaga
    61981 acgaaaagac attgcttatg acttctataa cttctcagag ggagaccaaa gtagtgaagg
    62041 ttcttgggct agtggcaaag cactggatgg cgaaggcgaa tatcaatggt tgaaagaacc
    62101 taagatcgaa ggcgctgcac caaaattaga accagtaaca ccaaaaatgt atgggacacc
    62161 tccattcaag aaataaatat ctacccgtag aaacacccgt tttttgttcc acacgctttg
    62221 tgagcaaaac tgactaaagt cattaatcat aaccaccctt caaaagggtg gttttctcta
    62281 gagctataag ctcaaatcac cggccagcgc ctaaagacgc tggctttcat attttatgtt
    62341 caagccatat tgcctttgcc agttttgccc acctcttaaa aggtttatgt actactcctt
    62401 agcccttaaa aggatcgtct tactctcgta cactcagctt atctaacgca ctatcatgtt
    62461 tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt ttgtcctact tttgtcacat
    62521 agtatccttc cacccaaaag tgtcgatacc caaatttata ttttagattt gcacaatctc
    62581 ttgatgatct cctgaatatt ggctcgatat tgattataaa ttatttttct tctatacttt
    62641 ggtgtgaata caatgtggta tttacacatc tactttgtac gggctaaact atttcccttt
    62701 attgctccac ccgcatagca agtggttttg tatttcaatc gctcggcgcg ctcaattagg
    62761 tctaaagaca ataaatctaa aaacccgctg cttcactgac agaaacaacg ggttttcaga
    62821 aaaaaatgaa atgaaaaaga tctcttacgg caatctattt tatttttttg attttttata
    62881 aatggaaaat gcttcaagaa gtccaagaaa taaagccgta taaaaacaag aacctatgaa
    62941 atctttattt attagatatt caacagaaat cccgataaca ctagccaaca atatatttat
    63001 cagaagtgta aaataaggat gctttttata gaatagaact agtgttgttt tcatagactc
    63061 tcctttcttc atatttccaa ttaatcacaa attcacggca ttactagaaa aattaaaggg
    63121 gcgcagaagt ggaaaaatac tcttcgactt tccctacaaa ccaggccatc ttgtgtttaa
    63181 taactatacc aaatctaagg tacctctaaa tctaaattcc atttgtggcg gtgacctcca
    63241 atttttaact cttctatctc ccatccagta tacataacct tgatacttcg accacttata
    63301 tatcgaacac acaaatacct atccattgca ggagtactac ctaacttagt tgctacttgc
    63361 atctaaataa cagctgaaaa gataggactt actcttagta aagtatctga actattaatt
    63421 tttaaactat catttttatc ctcaactaca tgcacatctg ttgcaacttc actttctgtc
    63481 tcatccagat aaatgattaa ggaatcggga attgcaatat cttcagctga cgctggaata
    63541 gttgagctaa attagtcatc atatctttat tataaaataa ccaacagcaa aaaatacaat
    63601 gaaaaacaaa cttggaacta atgtatcatt aataaatgtt gaaaaattca gcaactcatt
    63661 actgtatgat tttagcagcc caaccaacat ataccctata aaagctaata agatggttag
    63721 gaatccttta gaatttttac tcattcatag acacttcctt aaataacttt tattatagaa
    63781 ggtattgtaa ataataatat tgacaaaaag tgactcaaaa taaaaaaaac cttacattat
    63841 actggaatga actgtattga tagtgtacct aaaagctcta ctgacataag atcgtgtaat
    63901 cggtaaagta attttattaa ttatacggga tgtaatcaat tagagaataa actaatcata
    63961 aaaggtactt ttacattcat aattgtcgct ttgagattct actttggttc actatctgac
    64021 ccaattgcac attttcgatc attacaagta ttggtgtcgt tagaacaact aaataaaaac
    64081 tagcggtaga tttttcagaa tctatgctaa tttctgtatg gatgttccag tgatggcaaa
    64141 tttctctttt cattgttttt cttgtcgcat gatatgcctt tttgtgttat tcagttaaac
    64201 tatagaaatt ctttcctaga cagatcttga tcccaaattt tacgtttttg agcatcgata
    64261 tagtttaaaa tgacattact catatctaaa gagtttagat tttttaggac taaaactgtc
    64321 agttgttctt tttcttcttt ctgttaaagt attgtcgcga cttttcgtat acgttaagat
    64381 atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa tgacttgagg aaatcacctc
    64441 aatcattcgt ttaaaatgct atatattctt tagtttaaga tacggaaatt aatgccatcg
    64501 accacactag tagaatgaac gacatttact tggccttttt ttaatttcgt tatgtttagc
    64561 atataaaaat attatagagt ttacttacaa tgcttaaaac atatactgta taatctgatc
    64621 aattttagcc atgtcttgca atggtaagtt ttctattttt ttgagttttc gttccttaaa
    64681 atcaagagat tttaattgtg taataattac ttgtcctttt atatctagct cttcaggtaa
    64741 tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt ggacatatga ccgaaaacat
    64801 tgttttacgg ttaaattcat aacgagagac gactagtcct ggtcgtcttt tttgaatttc
    64861 ttttcctacg gagggatcaa aatcaatcca tacaatatca ccttttttag gaatataaat
    64921 tctttcttct gacactataa aatttctctc ccttcaggag ttaaatcttc ccattcgtct
    64981 ttctcataat actcggcctc ttccccacca ctaaatggat cttcaatttt tggaactaac
    65041 gtaattgttc catcatcaga atacacaacg atatattctt ggttttctga aggtttcttt
    65101 ccgttatcag aaggtaaagt taccactaca gaacttcctt gtaatctaga ttttgtaaca
    65161 agcattatca tcactccctt aataaaagta tactatataa cactaagtaa cgcaaaattc
    65221 ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg gatataaata
    65281 gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca agaaacattg
    65341 aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac cttcaatttt
    65401 gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat catgcccgat
    65461 tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg aaagttcacg
    65521 aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa
    65581 acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg tgagatcgta
    65641 gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt ggataaatgg
    65701 caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt aactttgaat
    65761 tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat aaataaaaaa
    65821 gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc gactgtgacc
    65881 ctatagatgc agtatatgca gcttatgagc atttcttctg ttctctttgt ttttgggatc
    65941 tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat tatgttatac
    66001 cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc
    66061 tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt catggattgg
    66121 tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt tttcatcctg
    66181 atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa acataaattt
    66241 gtctttttct tcattccact cattgggaaa acacaacgat tttgcaaaaa tggttgatga
    66301 aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg tcctttttta
    66361 aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc ctcttttctt
    66421 aacgtcacaa caagatttga cggaaaaact tctccagctg ctaattctgc tgcctgcttc
    66481 ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc tagatcaaaa
    66541 aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac actgcaacaa
    66601 gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg
    66661 acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt cacaatattc
    66721 aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg aaacggcagg
    66781 cttacgacct ctgggtaaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    66841 tgcaaaaatg tagaaagcta ttttcacaaa atttttctta atactcgaaa ctaagcgact
    66901 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    66961 ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt gatgcagggc
    67021 agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg gtattagctc
    67081 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
    67141 gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc gaaaaataag
    67201 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    67261 tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt aacaatcttt
    67321 ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact ttcaacaata
    67381 agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct tcttcatcgg
    67441 acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc cattgattga
    67501 ctaattctag tagcaacaga cggtattctt catccactgt actcaactca ataatcaaat
    67561 tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa atccagtcca
    67621 acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct tcgaacaatt
    67681 ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct ttttttgaag
    67741 aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata tctcggattg
    67801 acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag atcttttcct
    67861 ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata cagtaaattc
    67921 atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga cacagccctc
    67981 actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc gaggacaaaa
    68041 gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca cttgatctgc
    68101 aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag caccaattgc
    68161 atctgtcgct accacaacat tgaatccact ctctaccaat tcacgcatat gggaatcgct
    68221 acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt ttctcaattg
    68281 gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga tgatcgtatt
    68341 ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt cttccactga
    68401 ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt ctcctgcacc
    68461 gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa agagtttgta
    68521 tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat tgtatttttc
    68581 taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact cgacttgcgg
    68641 gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc cattcgttat
    68701 ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga tttgatctct
    68761 caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga tgacttcatg
    68821 agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt cgactaaaac
    68881 tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc ttgtcatata
    68941 cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta aacctacatt
    69001 ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat tcgttgttcg
    69061 tttattctcg cccttcaact gatccataat gattcgataa tcatctagag agatataagt
    69121 gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag
    69181 gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat tttctaatcc
    69241 aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg cttggattgt
    69301 tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt ctgctacttg
    69361 atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat
    69421 ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa gaacgacaac
    69481 tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc cagccccgat
    69541 gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat caatcaagta
    69601 gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag tattgatgaa
    69661 aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga tttgatggtt
    69721 agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg
    69781 ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa
    69841 gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat
    69901 acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt caccttttga
    69961 agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa tattttgata
    70021 tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa aaatatgata
    70081 accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa
    70141 agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa acaatcgtta
    70201 cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca tgcgcatctt
    70261 ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct aatactacac
    70321 cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt actgtggact
    70381 ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa caagaagcat
    70441 aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct ggaggagtga
    70501 ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg ccatggttga
    70561 tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac catttgctac
    70621 ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc tgcttattcg
    70681 aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact actcgtgaac
    70741 aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc ttttatatga
    70801 ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac tgttaaaaaa
    70861 ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca gcactcgatt
    70921 taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac tggtttattc
    70981 tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt ttactaacgc
    71041 caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg gctcatgtaa
    71101 gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat atcgaatacc
    71161 ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc gcttcatttc
    71221 ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta atcggatttc
    71281 ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga taccttttct
    71341 ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat catccagaaa
    71401 atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg tatctttccc
    71461 aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa tataacgaac
    71521 ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag gggaattcgt
    71581 cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg cgatcaaatt
    71641 atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca catctgtatc
    71701 gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac tttccatcgc
    71761 ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg
    71821 ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga atccaccagc
    71881 accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt ctagtgttcc
    71941 aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt
    72001 ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga taaaagcaat
    72061 atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat aataataggg
    72121 aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa attgagtgga
    72181 cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc cttgttcttt
    72241 cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt catgaccaat
    72301 cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta tccagtttgc
    72361 tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat atccttcaac
    72421 tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat ctttcatata
    72481 aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg ctgtgcttct
    72541 tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc gacaacttca
    72601 gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg ttcagtcaat
    72661 acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat tgctgtacgc
    72721 aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg tactattttg
    72781 ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat cgttgcttga
    72841 gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt cccaataggt
    72901 tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat attagacacc
    72961 tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg tattttttac
    73021 ttttattttt tgttttcatt caggttgcta acctttttct attttaacac gcaaaatcgt
    73081 cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt tgtgttcttt
    73141 agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa acgttgctgg
    73201 ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt ccatcttttt
    73261 gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa tggcttcttg
    73321 tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag aaaaatgttc
    73381 ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc cttccagtgg
    73441 atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg acatgcgtat
    73501 tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct gattcgggtc
    73561 gccgatccct ctgatgctaa agaatgattt tttaggaacc tctagtattt gcgggctttt
    73621 tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc ttcctccgcg
    73681 aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct aatcgtgttg
    73741 acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca gataacacgt
    73801 ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact ctcttttttt
    73861 acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc
    73921 agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt
    73981 aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc aatgggtaac
    74041 aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt tgaatcgcaa
    74101 ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag gtataatgat
    74161 cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc aaggtgcagc
    74221 gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc ccctcaatcg
    74281 acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag caattggccc
    74341 cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc tgtttcttgt
    74401 gcccgttttg tttctttctt tgattatcgg ctggtttgct attcccgaga tggcagtaca
    74461 aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt ttagtggaag
    74521 tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa tcatcggcgg
    74581 tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac tgatccgact
    74641 gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag tttatcagtt
    74701 tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag
    74761 tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc
    74821 tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg ctgggctgat
    74881 tctttctttg attggttggc tagcattatt cctactcata ggacacgctt cagtgggtgt
    74941 attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca gcaatatgat
    75001 gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg caatgttcaa
    75061 taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca ttatcaatta
    75121 cgtccagcat catacttccc ataactatga agtgtctact acattaggat caaaagcagc
    75181 attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct attatctgtt
    75241 ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg tttttttgtg
    75301 tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat tatcggtggc
    75361 gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa tgttgcttta
    75421 tttgatgaag ggacaggaca ggcaactaaa gctagcgctg ggattatctc cccttggttg
    75481 tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc ttttttccct
    75541 aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg gacacttttg
    75601 cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg aaaagaagaa
    75661 gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca agcacttccg
    75721 ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga tggaaaagca
    75781 tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa
    75841 gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg agaatacaca
    75901 gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca agaattaggc
    75961 tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac atcttataag
    76021 atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat tcctttcatg
    76081 gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga tttggctcct
    76141 actagcgaag cttataaggg attgactaag agtacagaac agttcttgac tgatccagat
    76201 cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac gtcagatttt
    76261 tccccatttt tcggcgtact gccagatgat ccaagcttac tggctgctag cggtctaggc
    76321 tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt cttaaatact
    76381 ggtcattggc ctacggaaga ttatcaaaag gacatctcca cctatattac gaaaaatcca
    76441 tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat ggattacttc
    76501 ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact ttcttttgct
    76561 tcatctggca agagatcttt tcctatctga gcttcaatga atcgtaaacg aatatcaagt
    76621 cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa atctggtata
    76681 ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt ttgttgcacg
    76741 attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc gaaatatcca
    76801 gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag
    76861 tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac tagctcatct
    76921 agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa tgttgcacca
    76981 aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga atcatctttc
    77041 atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc gggtgtcgca
    77101 taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac taggactttt
    77161 tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga agaaatggcc
    77221 ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca
    77281 aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac agagtgacct
    77341 tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc aaatacagcc
    77401 atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca ggcaataaag
    77461 ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag
    77521 taacgtaatc ctagcacagc cgctgtatta tggagagcat caaacaaaat gagtccattt
    77581 ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc ctcatcaaag
    77641 gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa
    77701 taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg cacagaaaat
    77761 tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg actttgatac
    77821 acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc
    77881 cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag tgtcctaaca
    77941 tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat gatcgttcct
    78001 ggactccctt ttgctagttg ataagctgtc ttcctatcaa aaacttcatg aacattatta
    78061 ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt tccacttgtg
    78121 atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc cctaaacaaa
    78181 aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc gctgtgggaa
    78241 gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag tttctcggtt
    78301 tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat gttttagcag
    78361 catcccatga accataacga gatgttacat attgttctgc tacacgttct tgattggcag
    78421 ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg taacgtccgt
    78481 tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt gcagagcttg
    78541 aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt tcaacaggtg
    78601 caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt tctgttgttt
    78661 tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt tcaccaacat
    78721 aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc attgagttgt
    78781 ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact gtataaactt
    78841 catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca gcaaaagttg
    78901 tacctaaaac aattttctta aatgaattca tattaaactc ctttttttct ccgtttttta
    78961 ttctttcgac gctattaact ttatcattta gaaatatttc ataggtaaaa gaaacatgac
    79021 atttaatgac aaaataacga attatttaat atttgtaaca tttaaacaaa tatttttttg
    79081 atacaaaacc ataaaagcct tgttgtataa gcaaagtaat cgtttttcta tgttttttta
    79141 tttttgaatg aaaaacgcag attctgtaac attcactgca ataatccata aaaaaaagca
    79201 aaaaaatttt tgatttttat caaattctct taaaaaaatg taaaaaatac aaaaaaacca
    79261 gtaattgtta caacaattac tggacaaaaa ctttgaataa tgttaactat ttttttattt
    79321 taactttctc cgcaataatt tctgggcatg ttccaattcg tttataacga cttcttctgt
    79381 ttctttagct aatatttcat ctaatacatc aagccatttt tccggttctt ttgttcctag
    79441 acgtccaata gcccaagctg ctgttcctct gatgacaggg cggacatctg ttaaacattc
    79501 tgaaatctca ggcaatgccg ttcgatcacc tagattagct aaagcaatca atgcgtttct
    79561 ctgcaaaggt ttcttcccac gccatgaacc agctaaatgc ccaaattgct gtttgaagtc
    79621 tttattagac atcgaaagca ttggctttaa ttttggatat atttcgtcta cttttggttc
    79681 catctcttca tgaaaatgaa aatcttttcc ttgattgtaa gggcagacta actggcagat
    79741 atcacatcca taaatgacat ttcgcatttt tttacgatat tcttccggca tcatcccttt
    79801 tgtctgagtt tgataagaca aacatcgctg ggcattcatt cttccgtcac caagcaatgc
    79861 ttttgtcgga caagcagtca cgcagcgcat acagtcaccg caaccaaatt ctcctggttt
    79921 atcaggctca aatacaatat tagtaacgat ctcaccaaga tagacaaatg aaccgaactc
    79981 ttcagtgatc aacaatccgt ttcgtccaat aaatcctaaa ccagctcgct gtgctacagc
    80041 gacatcgatc agttctccag tatctacttg cggagctaga cgccattcct cttctttttg
    80101 ccatttttct gctcttgttt ggatgaattc gatcaatcgg ttcaaacggt ctctgagtat
    80161 atcatggtaa tcgatccccc aagaagcacg agcaaactgc ccgcgttttt catccctagg
    80221 cacttcttca tggatcctag taggatacgc aagtgcaatg gaaatgatcg atttcggctg
    80281 atcaaatgtc atttctggat aaagacgctc atcgatattt ggatgttcaa agccagaagt
    80341 atggccagct gctttttgtt cttccaacga ttcttttaat gaatcaaaag gttccgccgt
    80401 ggtgaacccg attttgtcaa ttcctaatcg tttgctttct tgtatgattt cttcttttaa
    80461 tgtcatggtc ttcctcgctt gctgttaacg gctgtataac ccataatata gtttttcttt
    80521 tttcagctgg acaaactgga tctgctgatc ggcaatcttc ttttcttcca tccatttttt
    80581 tcttgcagca aatacttccc aataatttgt tgaaattaat tttcttttgt tatctaatgc
    80641 attaaaaaaa ttcaatgaaa ctggttttgt acttaacagt tcatggagcc ttacctcttc
    80701 ccatggcgaa aactcagaca gatcgcggga atacaatact tcttcataga gatttttttg
    80761 ctgtgtgtac gtgaatgttt gcgggatttt cccaggccta gcaatttcta ctagacgatt
    80821 ataaaacata ttcattaatt tgctgttggc aataaattga cttttataat aggccgactg
    80881 gatctttttg attactcctt ctgctttgat gactgaaaat aatgcaggcc atgctttgac
    80941 ggttatttcg tcgttttgtt taactaatac gccaggtgtg aagccgactg gagaaaggaa
    81001 cacaaaataa ccgataccgg tattttcatc taaaggctga aattcatcaa acaaccattt
    81061 gcctatgtac agctcttcat ctggttctgt tgtctcattt tgatttgcta tgaataattt
    81121 agctaattct gcttcaagaa attgaaggtc ttgatccttg tcggtatttt cctgctggct
    81181 gatcaatcga aagacttctt ctttaatcgt atatttatct ttacgtgtaa aattcatagc
    81241 cacaccttcc ttccttactc taactataac ggaatttctt caaaaaaggt atctgtacga
    81301 cgaaaaacag cataattttt tttcatatct cttataaaaa atgcaaggtc tgctcctcaa
    81361 gctagacctt gcacttttca gctttctttt gtttgtgaca aaccttcatg gatcgtttca
    81421 atattccatt tcatcatctg atagtaagaa tctccttgtt ctccttcttt tgctatggaa
    81481 tcagtgaaca gtttatcata gattttcaaa cccgtttctt ttgctacccg ttccatgctt
    81541 cttgagtcta cactggtttc cacgaataaa accggtactt cttttgctct tatctgatca
    81601 atgatcgctt tcatttgatc aggcgtacct tgactttctg tattgatctc ccatatataa
    81661 gctgctggca aatcatatgc tcgtgaaaaa tacttgaatg ccccttcact tgttactaat
    81721 aattttttgt tctcttcgat agaagcaaaa ctttccttcg cttgtgtatc taattctttt
    81781 agtttttcca catatgcttc tgcatttttt ttatagagtg tcgcattttc tgcatctttt
    81841 tcagagaata tacgtgcgat ttcctccaca tattttattc cgttagatag gtctagccaa
    81901 gcatggggat ctgcttttgt atgttcttca ccgctagtta aataaagagg ttctacattt
    81961 ttgctaactg caaaataatc ttttccttct tcttttttag ccgtttccat caagttatcg
    82021 aaccagctgt tacctgtttc aagattcaaa ccgttgtata aaataacatc tgcatcgctt
    82081 gcctttttga tgtcttctgg tagtacttca tattcatgcg gatctgttcc gactggtacg
    82141 atactgtgga tatctatttg atctgttccg acctcttttg ccatgtccgc caagatcgaa
    82201 ttggtagcca cgactgacaa cttttcttta cttccttcat ccgcttggtc agaagcctgc
    82261 tttccgcaac cggctaatac caagaaacta actgccaaga ttaaaaagag tgattttttt
    82321 gttttcatct atctgcttcc tttctgaaat aaaattcctt ttgttggaga aaataaaaaa
    82381 gcaaggataa aaaatgcggt acatgctaac acgatcgtag ctcctgatgg ccaattgtaa
    82441 ctataactga aaaacacacc gacaactgca cttagcatac cgattcctgc agctgtaatg
    82501 atcattttca gtaaatggtt cgttagcaag taagccgtgg cggctggtgt gatcaacatg
    82561 gcaatcacca agattgttcc tactgtctgt aaactgacta cagccactaa tgtcaaaaag
    82621 aacatcaaaa gataatggat cagcgatgtg ttcagcccat aagcttttgc cattgtcgga
    82681 tcgaatgatg tgatcttcaa ttctttataa aagaaaaaga caaatatcaa gacgattcca
    82741 ctaacgagtg ctgtcagcaa caaatcgctt tccctaacag ctaagacatt cccaaataaa
    82801 atatgataca aatctgtcgc actctgggca tatgaaatca agataatccc taatgcaaaa
    82861 aaggagctaa agacaattcc aatcgctgta tcatttttca atcggctccg ctgagtcacg
    82921 aaaccaatca ctgctgctgt tagcatccca aaagcagtgg ctccaaagat gtaactaaaa
    82981 ccaaacatat aagaaatggc tacacctgga agaactgcgt gagagatagc gtctcccatc
    83041 aaagacatcc ctcgtaaaat aataaaagaa ccgatgactc cagaagcaag tcctacaagc
    83101 atggaagtga ccaaagcgta ttgcaggaat tgataattca ttaatccatc aataaaggat
    83161 tggatcattt tttctctcct ccttttacta aatgtctgat gatctcacca taggccaatt
    83221 gaaggttctt cgatgtaaag acttcttgta ctggaccgga agcgatcagc tgtttattta
    83281 aaagaataac ctcatcgaaa tattcttcta ctttatgaag atcatgatgg acgatcaaaa
    83341 tcgtttttcc tgaattcttc aattcctgca agatgtcaaa gatctttcgt tcacttaacg
    83401 catcaatccc tacgaatggt tcatctaaaa agatccattc tgctccttgg gctagagctc
    83461 ttgcaataaa aactctctga agctgtccac ccgatagttc gctgatctgt cttttggcat
    83521 attcttccat ccctactttt ttcaaagctt gtcttgcacg ttctttttct tttttcccag
    83581 gtctttgtcc aattcgtaaa gatggatatg ttcctaaaag tacaacgcct aatacatcaa
    83641 ttggaaagga aagatccaat tcacttcgct gttccacgta ggctattttt ttacggactg
    83701 agtttaccga ctgtccagaa aaggtgactt taccagtagt agctggtatc aactctagca
    83761 aagatttcat aaatgttgat tttcctgcac cgtttggtcc gataataccg gtaatttttt
    83821 gtggatttat cgtcaagttg atagttttca aaacagtttt cccttgataa gcggctgaaa
    83881 ggtcttgaat tacaatggcc gtacgttcca aatcttacac tcctttttag gtttacctaa
    83941 ctttttattt aaatatagtt taactaaaat ggaaagcatt ttcaagtaga atgatgaaaa
    84001 agtttttttc gaattttcct cgcattttat gaaaattttc tgtagaatca catcaagagt
    84061 tcaataaggc taacctaata atgaaaaaca tgtgttgaaa cgtttgatta ttttttcttg
    84121 ttgactcaac aacgaaagga gtttttgtat ggaagaagaa aagttaactc gaagtcttag
    84181 tgcccgtcat atccaaatga tcgcactagg tggaacgatc ggtgtcggtc tgtttatggg
    84241 tgcctcctca accatacgtt ggacagggcc ctcagtgatg ggagcctatg cgattgcagg
    84301 attctttttg tatttgatta tgcgtgcttt aggcgaaatg ctttacgttg atccttcgac
    84361 tggttctttt gcaaattacg cgagcgagta catccatcct gtggctggct acttgaccgc
    84421 ttggagtaat atttttcaat atattgtcgt aggaataagt gaagtgattg ctgtaggatc
    84481 ttatatgaat tattggtggc ctgatttgcc ggcaattatt cccggaatca ttgtcgtctt
    84541 gtttcttatg ctggcaaatc ttatttctgt caaggcgttt ggcgaattag aattttggtt
    84601 ttcgatcatc aaagtcataa cgatcatttt gatgatcatt gcaggtcttg gggtaatctt
    84661 atttggattt ggtaatcatg gtcaggcagt agggatttca aatctttgga aaaatggtgg
    84721 attcttcact ggaggcgtca aaggattttt ctttgcatta tcgatcgttg ttgcttccta
    84781 tcaaggaatc gaactgatcg gaatgactgc tggtgaagca gagaatccta aaaagacgat
    84841 cattgaagct gtccagtcca ctatcggtcg aatcttgatt ttttatattg gtgctatttt
    84901 tatcattgtg agtatctatc cgtggaatca attgagtgag attggttcac cttttgtaca
    84961 aactttttct aaagtcggca ttacatttgc tgcaggactg attaattttg tcgtgattac
    85021 agcggcatta tcaggttgca attcagggat tttcagcgca agtcgtatgg tctatacact
    85081 tgccataaat ggaaagatgt ctaaaaaatt tctgaaacta tctcgtcacg gtgcgccttt
    85141 ttatcctgtc gtagcaatct caggtggaat cttaattggt ctgatcttaa attatcttct
    85201 accaatgttc atcaaccagt cagaaaatat tttcgttttc gtatacagct caagtatttt
    85261 gcctggtatg gttccttggg tagtgatttt gatcagcgag attcgcttca aaaagaagca
    85321 cgcagataaa atgcatgatc atccttttaa aatgccacta tctccttata cgaattatgt
    85381 gacacttgct tttttagcta tcgtattgat tttcatgttt atcaatcctg aaacgagaat
    85441 ctcattgatt attggactcg ttttcttgct ttacatgacg atccattact ttgtacgcga
    85501 aaaaagaacc ggaaaataaa aaaagaacga agcttcacct tagtggaagc ttcgttcttt
    85561 tttattgctt ttatcaattc aattttagcg tctcaccggg agtcaggaaa taattctcca
    85621 tgctcatccc attcagcgac aagatcgttt ctactggtac gccagttcgt tcggctattt
    85681 gatttggacc ttctccttcc aaaactacgg ttgtcgcagc ctgtgaagtc gcgctgttgg
    85741 tattagtttc agaggaatct gttgtgtctt gtgttgttcg agcttgcatt tcagaagctt
    85801 ctaccgactc tgctgtctca gaatcagaag atgttgtgtc ttgtgtgctt gttgactctg
    85861 cttgaagaga ggcgctagtc tgcaaataaa tatcggaaat cttagcgaca tgattcgtat
    85921 aattagccga taattgattg atcaaagtat ctaagtctac accgtcttta ttttctgctg
    85981 cctgatacgc tgcacgtaat tttctgatcc cttcgtcgga gattgcttga agctcacgtg
    86041 ctttttggtt agcaactgct gataaaccag ctattccctc ttgatttccc tgaatttctg
    86101 tctggtattc ttcaatcaat gcaggcgtct tttcttcgat ctttttcgaa taatcattta
    86161 cgattgcttg aagttgctct ggcaaaccat ttgctgtttt ttcttcagaa gtttgtgtct
    86221 cttttgtttt agttgatgac tcacttgttt gtttcgtctc tgttttcttt gtcgttgttt
    86281 ccgttatggt cgtacttttc gcttctgacg aattttttcc atgaaaatat agacccgcaa
    86341 ctcctcctgc tatgatcaaa aacacggcaa ctgctgcccc aatcatgatc atccgcttac
    86401 gagctgcctg tttttgctgt cttcttcggg tcatccgaac attttctccc cactcatttt
    86461 catcatacat ttttttcacc accaattttt atgtataata gcgttatcaa acgcttcata
    86521 ttcttattgt agcaaataat tgtcgctgtg cacattttta tctttgttat ttataagaaa
    86581 atttcaccag ttgagatgat tccaagattg tccgtcgatt tctccttgca gctgcattgt
    86641 tcgttcgtct atctcagcca tcagtttctt tatttcatat aagagcgcac ggtcttgata
    86701 ttctgatttt tcgcttaatt catctaattg ttggatcagc caatcatttg tcataatgaa
    86761 gttcctttca tttcttgcag tgtaaaggtc aagtcttcga tctgctcttt tgcctcagtc
    86821 agtgcttcta cgatatatgc ttgtttttca gattcttctt cctgcatttt ctgattttcc
    86881 tgtactcttt gaataaacca gtcaatccgc tgagtcagcg atggtttgcc ttccaatgag
    86941 agatactgct gataagcaat tagcttctcc caacgttttt cctgggataa atacataaat
    87001 tgtgctaaaa cgaatggcgg aagataggtc atatggaatt tatttgcgat tcgctgatac
    87061 ggcctcaaaa attcggaaat cgtatattct tcttttcctc cagcttggta ttctttttcc
    87121 gccacacctg ttgtcaatac gattcctagt tctttccctt ttaatacatg tgctgctcgt
    87181 tccaagacat tatcttgcca aatttttaaa tgagcaggag aactatacca gtaaaacgga
    87241 aactggaaaa tgatccgatc atgctgggcc aagagctgta gttcagtctc tatatctatt
    87301 tttccgtctg gataacttga ttccaaatga tgccaagtag ctggtaaacc agcagaagct
    87361 tctttgaaaa attgctgatt taaagattct tcgatcgttg gatggctaat gatgataagt
    87421 gtacgcatcc cttttcctcc tttttagtct tcgctttaca gtgtagcata accgccacaa
    87481 gtttcttttg ccgatgcttg tcattttaac cagactaatc aggttgtcca agaaactgcg
    87541 gtttgttttt ccaactgttt tttcaaggta tccaaagcaa tattcttaaa attttcgttt
    87601 ttcatacgtt tacgatagtt cgtagccgtt tctccggtac tctttttgaa cattcggcta
    87661 aatacgacta gactattgaa ccctacttga taggcaatac tttccacgga ttcatccgtc
    87721 agagaaagaa gaaacgcccc tcgattgatc cgtaccccga tcaaatattc cttgatactg
    87781 gtacccatat gatttttgaa tactcgcgac aaatggctcc gattgaccgc taaatgctgg
    87841 gcgatttcct caacagacaa tttttcagca taatgtgcct cgatgtattt gactgtttca
    87901 acagctaaag aggaatgaac cttttgtaca ccaccgctga cctttccgcc atcttttaaa
    87961 aggactgata aaaaacggta ggttaatgct gttaactgta attcattttc caaggtttct
    88021 tccgtatctt tcatgcattc taaaataatg tcgatatatt tttgtatgtc agaagagacc
    88081 atcgtgtatt ggtcttctct gaaaagcgac tgccgaatga tttcttctgc agcactccca
    88141 ccaaatgaaa gccagcaata catccacgga tcttctccat ctgataagta aaacgtcgag
    88201 tctcctggac gaatcaagaa cagatcacct tttttcaatt ggaattgttg atttttcaca
    88261 tgataagatc ctttgccttc catcacgaca tgcaggatgt aatcattgcg aatagcagga
    88321 ccaaaagaat gaaacggcat cgttttagag aaaccacaaa aatcaaaata caattctttt
    88381 tctacttgtt gatgttcctt ttttaaaaaa atcgattgct ccacgtctat ccctccttac
    88441 tcctttagta taatgcaaca aaaagttaaa taaaagtcac agaaatatat taaaaaggaa
    88501 gcgatttctt gttaagttta agtcattaaa agatctttgt tattcgttgt aaccggttta
    88561 cttagtatta tatattgaac aggagaggaa tacagattat gaaaagcaaa atgatccaac
    88621 gtgtgtccta cgctttaggc gcttttggac acgacacatt ctatgttaca ttaagcactt
    88681 attttatggt attcgtgaca agccagatgt tcgcaggaac agataaggca acgaatgcaa
    88741 aaatgattgc tacagttaca agcttagttg taggtatccg cttagttgaa attatttttg
    88801 atcctttgat tggtggcatc atcgataata ctcgtacaaa atacggtaaa ttcaaaccat
    88861 ggttagttat cggcggcgtt gttagttccg tactcttggt agcaatcttt accaatttct
    88921 ttggcttagc tactcataat acgactttat ttatcatatt atttgcaatc gttttcatct
    88981 tattggatag tttttattca tttaaggata tcgctttttg gtcaatgatt ccagctttga
    89041 gtacggaaac aaaggaacgt gaaaaatttg ctacgtttgc tcgtttcggc tcttctctag
    89101 gtgcaaatgg tactactcta gtcgttgtcc ctatcgttac tttctttacc tatcttgtta
    89161 ctcataaaca tattgaaggc gcttcaggct ggttctggtt cggtgtgatt gctgctgtta
    89221 tttcaggcgg tactgcgatt atcactgcct tgggcacaaa agaagtcgat actgcgatcc
    89281 gtcagcaaaa tgaaaaagct gggatcaaag atgtgttcaa agcagttgct caaaatgatc
    89341 agttgatgtg gcttgccctt gcttatatcg cttatgcgat tgccaatgca gcaacaactg
    89401 ccgtattatt ctacttcttc aaatatgtac ttggccaacc aactgaattt tggatcgttg
    89461 gtctagttgc tgccttactt ggtttagtag ctgttccact attcccaatg cttacacgcg
    89521 tcatctctcg tcgctacgtt taccttggag ggatcgcaat gatgatttct tcttactttt
    89581 tctttacgat cgcaggttca agtttgccaa tcgttatcat cgggctagta ttgttctact
    89641 tccctcaaca attgattttc ttatccgctt tgatgacgat cacagactct gttgaatatg
    89701 gtcaatggaa aaatggtatc cgtaacgaag ctgttactct atcaattcgc cctttactag
    89761 ataaaatcgc tggtgctttg tctaacggga tcgtagggtt tgtcgctatc gcttctggga
    89821 tgactggtaa tgctactgct gctgatatca ctgctcatgg cgtaacgact ttcaaagctt
    89881 atgcattcta tgctcctgct gctttgatga ttttctccgc tattattttt gcttggaaga
    89941 ttacattgac agagaaaaaa catgcacaaa tcgttgagaa attggaaaac aaattggctc
    90001 cagctgatac aatggaagat taacttcacg atgcagtagt tccttcccat taagaaagga
    90061 tcttgaaaat gaagattaca gaaaaagatt ttggccaagg ttatcacttg ataaccttgg
    90121 agaacaaaaa caagctagca ctttctatct ctgatttagg tgctagaatc gtcagtttaa
    90181 aatcaaatga tcgagaatta gttttgggat ttgacaccgc tgaagaatat atcgaaaaag
    90241 atccgtatat cggtgcttct atcggtcgca ccgcagggag aatcgaaaat ggccgctttt
    90301 ctctcaatgg aaaaacttac caattagcca cagatccaaa aactggacat aatctgcatg
    90361 gtggtgctcc tggatttgaa ttaaaaaaat ggtcttatgt cattttgaat ggcgaaaatg
    90421 aagcttctgt catttttact actacaagtc ccgacggaga acatggtttt ccaggtacaa
    90481 tggatgtgga aatccgctat actttgacaa aagataatat ctggcgagtg acctctcgcg
    90541 gcaccagtga ccaagatact ttattcaatc cgacaaatca tgtttacttc aacttgacgg
    90601 gagatgcttc tcagtcaatc gatcaacatg agctttggct caatagcgaa gcttatgctc
    90661 ctttgcgaac agatagtatt cctatagggg taaaagaaaa tgctgcagga agtgctttcg
    90721 attttcaaat acctaaaaaa ttagcgtccg tttttgctag tgatttggat caaaaaaatc
    90781 ttgttgacgg aatcgatcat ccatttttct taaaagaaac cggcttgggt aaagaagccg
    90841 ctcgcttgac tagccccgat aaaagaatcc aagtagatat tgctacagat gcttctagtg
    90901 tggttatttt cactgcaaat ttcggcacag aaacaccaga aatgcgcaac cgaaaactgg
    90961 cacatcacgg tggcatcaca tttgagactc aaacagctcc aggtgcagag cgcttctcag
    91021 catttggttc gattcattta aaagccggat cagtatttga aacagtaaca gaattcaaaa
    91081 tcaaaacaag aaaggaataa ctcatcatgg aaatcaaaag aagcttaaac acaaaatttg
    91141 ccgaaacatt cggacctaaa aaaactacac aatacttctc accaggacgc atcaatttga
    91201 ttggtgaaca cacagattac aatggaggcc atgtcttccc cgcttccatc acttatggaa
    91261 cgcaaggtgt agcagcacct cgagacgata ataaaatatt agtttattcc acaaactttg
    91321 acgatatcgg tgtcattagt tttactttag atgagctaga atatgataaa aaagcaaact
    91381 gggcaaatta cgtaaaagga atggtcctta aattaaaaga agctggacac tcaatcgatc
    91441 atgggtttga gttattagtg gaaggaacaa tccctaatgg tgcaggactt tccagtagcg
    91501 cttcattaga attattagta ggtgttgtat tagaagatct gtttgattta aaaacagatc
    91561 gcttggaact agtccaaaca ggtaaaaaag tagaaaatga attcatcggt gtcaattcag
    91621 gtatcatgga tcagtttgcc atcggcttcg gtgagctaga tcatgcaatc ttattagata
    91681 caaatacgct gaaatacgaa atggttccag tgaaactaga tggttatgca atcgtcatca
    91741 tgaatacgaa taagcggcgg gaattggcag attcaaaata caatgaacgc cgtagcgaat
    91801 gcgaagaagc cttggcacgt ttgcaaaaaa ctttgcctat ctcttcttta ggagacttag
    91861 atgaagaaga atttgaatca actattgacc aaatcggcga tgatacattg atacgcagag
    91921 ctcgccatgc cgtttctgaa aatcagcgga ctttgaaggc aaaagctgaa ttagaagctg
    91981 ggaacttaga agcatttggt caactgttaa atgattctca ccactctctt cgctatgatt
    92041 atgaagtaac tgggatcgaa ttagatacac tagtagatgc tgctcaaaaa caagaaggtg
    92101 tcttaggtgc acgaatgaca ggagctggat ttggtggctg tgcgattgct cttgtcaaag
    92161 aagaaaatat acctgccttc aaaaacaatg tctatgatga atacatgaaa gttgtcggct
    92221 atgctccgga attttatgtt gcacacattg gaaatgggac aacaaagatc gatccagaag
    92281 ctgttcatgt ttgataaaaa aatcaacgat ctcagctaaa aacaaataga taaaaataac
    92341 ctctcagcaa tttccaaatg tgagcctttt gctcgtattt gaaaattgct gttttttatt
    92401 gacacggaaa gagaacgtgt gttctaattg tgttgaagag gtgattgtta tgtgggcgaa
    92461 tcaaaatccg gtatttgttt atgaaaaaga accttcaaga gatattttat gtattgactg
    92521 caaatctttt tacgcctccg tagagtgcgt tgaaaaggat cttgatcccc ttacaactaa
    92581 attagtagtc atgtcctatc cgtccgattc gacagaaaca cggggtagcg gattaatctt
    92641 ggcttccagt cctgcagcaa aaaaagctta cggcatcacc aatatcagcc gtgccagaga
    92701 tcttcccttc ccctatccaa gtgatttata tattgttgcc ccaaggatgg cctattatat
    92761 ggaaaagaac agagaaatca attctatcta taaaaaatat gcagacgaag cgaaccatca
    92821 tgtctattcc gtcgatgaaa gcttccttga tgtaacaaat agcatggcac tctttggtaa
    92881 aacaaatgcg tacgacatgg ctaaactgat ccataaagat gtgtttgagc agaccggaat
    92941 ctacacgact ataggaattg gcgataaccc gctacttgct aaactagcct tagacaatgg
    93001 ctcaaaagac ggaaagcaaa tgtttgctga atggcgttat gaagatgtac cagaaaagtt
    93061 atggaaaatc tcagatatca ctgacttttg gggaatcggc aaacgaacag caaaacgtct
    93121 gaaacaaatg ggtatacatt ctgtttatga tttagctcac gcggattatt atcacttgaa
    93181 aagcaagcta ggggtcatcg gtactcagct ttatgcccat gcttggggca tcgatcgaag
    93241 ttttttggga caagaatata caccaaaatc aaaaagtatc ggaaatagtc aaatcctaga
    93301 tagagattat acaagacgag aagaaatcga gatcgttatc aaagaaatgg ccgaccaagt
    93361 cggcactcgc ttgagacgat ctgcagcaaa aacacaagta gtcagcttat gggtaggata
    93421 ctctttaggc tatcttgatc caaatggcaa gaccgggttt caccagcaaa tgaaaatcga
    93481 agcgacaaat gctagcagag atcttgcaga tgctttattg accattttcc accagcatta
    93541 ccactaccag gatattcggc gtgtaggcgt gaactgttcc gacctcgtat acgcgacagg
    93601 tttacagctt aacctttttg aagatcctga aaagcagata aatgaagcga aagttgattt
    93661 tgttgtcgat acgattagaa aaaaatacgg ctttcaatca atcatccatg cgcatagtct
    93721 cttgcaagga ggacgcgcca tcgcacgtag ttctctcgtt ggcgggcatg caggtggtat
    93781 gagtggatta gaaggtggaa aagatgagac ggacagcaaa aaaattcatt gactataacg
    93841 agtatcgaga tcggccattt ggattaaaat gggggactgc ttttgcgatg gaagaactag
    93901 tcaacggtat caaaaaaatg aagaagaagc acgcaaacag cctcttccta aacaacaaat
    93961 gacattgcaa gaaatcgata gtatactgac agaagccttt ttatctcgta aagcagtaat
    94021 cattcagctc aatttaaaag atgaatttgg aagactcttg gatgaggtcg aagggcactt
    94081 cattggtgaa gcctatgaag attactttat gattgaccat cagccgatat tgtgggaaga
    94141 cgtacggtat attgagttaa aggaaacaaa aaaatggttt gtgacatgag aggttgtgac
    94201 agaagtgatc aacttcaaaa aataagaaag gatttttcga aaattgcttc ttaaattttt
    94261 gtggaatttt ggcttatttt tcgaagaagc aacttctgga acaacgtata ttagatatca
    94321 gaggttgtga aaaagtcgct tagttttgag taacaagcca aggcgctgat ttcacaacct
    94381 ctgtctatct atccatctat tcggtaagat tcatgcatga cgtcttcttt atttagtatg
    94441 tagagccctt tttcttctga ccattcaaca taaatcagct gttttacatc tgcatagcct
    94501 tctttcttca agttctccat aagccatgct tcagtcaatt gattggactg aagcacttta
    94561 tgctgaattt gcccttcatc tacaagcagg atcgataaag ctttgtcttc atctgatttt
    94621 ttcaatacac tcatttgacc ggcattctct aggataacat gtttggcatt ttcaagaaaa
    94681 aagcattctt gctgtcttaa gattgctcgc aactgctcca tctcgatatg gttatttgtc
    94741 aactctgtca tgttgatgac gccgtctttg attagaacag atggctcgcc tttgacccaa
    94801 cgattgacct tttcattttt ttggacgata cgttcgatac cataaatcat tactgcccaa
    94861 agcgcaatgg caaataggac atgccctaca tggacattgt catcataggt tgattcttct
    94921 aagatcccac ctaaaacaag cgtatagact aaatcaaacg gtgtgatttc tgacatggat
    94981 ttcttcccaa gcaaacgtac cactaataat aaaccgatca acccgaacac cagtttcatt
    95041 gtaattgaaa aaatcatttt ttctccccct aactatctgt taattaaatt atatatgctt
    95101 tctcacgaac acgcatggaa tatacaagga agaaatgtat ccttttacta aaaaaaggac
    95161 tgtgacactg tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcctt
    95221 ttttttatgc tctaaaatga tatttttaca caaccgttac ggttgtttta cgaaattgga
    95281 cg
//
LOCUS       DAZUKT010000003        63866 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000003 DAZUKT010000000
VERSION     DAZUKT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.003"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..616)
                     /gene="dhaL"
                     /locus_tag="ACG816_000199"
     CDS             complement(11..616)
                     /gene="dhaL"
                     /locus_tag="ACG816_000199"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4919120.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="ACG816_000200"
     CDS             complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="ACG816_000200"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4919121.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1629..2024)
                     /gene="dhaM"
                     /locus_tag="ACG816_000201"
     CDS             complement(1629..2024)
                     /gene="dhaM"
                     /locus_tag="ACG816_000201"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4919122.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2178..2498
                     /locus_tag="ACG816_000202"
     CDS             2178..2498
                     /locus_tag="ACG816_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4919123.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2610..3467)
                     /locus_tag="ACG816_000203"
     CDS             complement(2610..3467)
                     /locus_tag="ACG816_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4919124.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3575..4879)
                     /locus_tag="ACG816_000204"
     CDS             complement(3575..4879)
                     /locus_tag="ACG816_000204"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4919125.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4876..5385)
                     /locus_tag="ACG816_000205"
     CDS             complement(4876..5385)
                     /locus_tag="ACG816_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919126.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5560..7695)
                     /locus_tag="ACG816_000206"
     CDS             complement(5560..7695)
                     /locus_tag="ACG816_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4919127.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7778..9433)
                     /locus_tag="ACG816_000207"
     CDS             complement(7778..9433)
                     /locus_tag="ACG816_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4919128.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9435..9749)
                     /locus_tag="ACG816_000208"
     CDS             complement(9435..9749)
                     /locus_tag="ACG816_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4919129.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9919..10179
                     /locus_tag="ACG816_000209"
     CDS             9919..10179
                     /locus_tag="ACG816_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4919130.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10193..10507
                     /locus_tag="ACG816_000210"
     CDS             10193..10507
                     /locus_tag="ACG816_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4919131.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11237..11791)
                     /locus_tag="ACG816_000211"
     CDS             complement(11237..11791)
                     /locus_tag="ACG816_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4919132.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11917..13846)
                     /locus_tag="ACG816_000212"
                     /pseudo
     CDS             complement(11917..13846)
                     /locus_tag="ACG816_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14152..14868)
                     /locus_tag="ACG816_000213"
     CDS             complement(14152..14868)
                     /locus_tag="ACG816_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4919133.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14869..16692)
                     /gene="glpO"
                     /locus_tag="ACG816_000214"
     CDS             complement(14869..16692)
                     /gene="glpO"
                     /locus_tag="ACG816_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4919134.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16706..18202)
                     /gene="glpK"
                     /locus_tag="ACG816_000215"
     CDS             complement(16706..18202)
                     /gene="glpK"
                     /locus_tag="ACG816_000215"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4919135.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18338..19798)
                     /locus_tag="ACG816_000216"
     CDS             complement(18338..19798)
                     /locus_tag="ACG816_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919136.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19788..21083)
                     /locus_tag="ACG816_000217"
     CDS             complement(19788..21083)
                     /locus_tag="ACG816_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4919137.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21415..22092)
                     /locus_tag="ACG816_000218"
     CDS             complement(21415..22092)
                     /locus_tag="ACG816_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4919138.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22122..23213)
                     /locus_tag="ACG816_000219"
     CDS             complement(22122..23213)
                     /locus_tag="ACG816_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4919139.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(23314..23748)
                     /locus_tag="ACG816_000220"
     CDS             complement(23314..23748)
                     /locus_tag="ACG816_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4919140.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24332..25789)
                     /locus_tag="ACG816_000221"
     CDS             complement(24332..25789)
                     /locus_tag="ACG816_000221"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4919141.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25786..27681)
                     /locus_tag="ACG816_000222"
     CDS             complement(25786..27681)
                     /locus_tag="ACG816_000222"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4919142.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27739..29514)
                     /locus_tag="ACG816_000223"
     CDS             complement(27739..29514)
                     /locus_tag="ACG816_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4919143.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29564..31060)
                     /locus_tag="ACG816_000224"
     CDS             complement(29564..31060)
                     /locus_tag="ACG816_000224"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4919144.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31254..32876)
                     /locus_tag="ACG816_000225"
     CDS             complement(31254..32876)
                     /locus_tag="ACG816_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4919145.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33204..34517)
                     /locus_tag="ACG816_000226"
     CDS             complement(33204..34517)
                     /locus_tag="ACG816_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4919146.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34762..34986)
                     /locus_tag="ACG816_000227"
     CDS             complement(34762..34986)
                     /locus_tag="ACG816_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919147.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35006..35428)
                     /locus_tag="ACG816_000228"
     CDS             complement(35006..35428)
                     /locus_tag="ACG816_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4919148.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35570..36280)
                     /locus_tag="ACG816_000229"
     CDS             complement(35570..36280)
                     /locus_tag="ACG816_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4919149.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36298..36795)
                     /locus_tag="ACG816_000230"
     CDS             complement(36298..36795)
                     /locus_tag="ACG816_000230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4919150.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36832..37284)
                     /locus_tag="ACG816_000231"
     CDS             complement(36832..37284)
                     /locus_tag="ACG816_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4919151.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37419..38024)
                     /locus_tag="ACG816_000232"
     CDS             complement(37419..38024)
                     /locus_tag="ACG816_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4919152.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38474..39094)
                     /locus_tag="ACG816_000233"
     CDS             complement(38474..39094)
                     /locus_tag="ACG816_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4919153.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39096..39965)
                     /locus_tag="ACG816_000234"
     CDS             complement(39096..39965)
                     /locus_tag="ACG816_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4919154.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39988..40395)
                     /locus_tag="ACG816_000235"
     CDS             complement(39988..40395)
                     /locus_tag="ACG816_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4919155.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40414..41703)
                     /locus_tag="ACG816_000236"
     CDS             complement(40414..41703)
                     /locus_tag="ACG816_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4919156.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(41730..42032)
                     /locus_tag="ACG816_000237"
     CDS             complement(41730..42032)
                     /locus_tag="ACG816_000237"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4919157.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42057..44150)
                     /locus_tag="ACG816_000238"
     CDS             complement(42057..44150)
                     /locus_tag="ACG816_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4919158.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(44639..45403)
                     /locus_tag="ACG816_000239"
     CDS             complement(44639..45403)
                     /locus_tag="ACG816_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4919159.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(45514..46383)
                     /gene="mdcB"
                     /locus_tag="ACG816_000240"
     CDS             complement(45514..46383)
                     /gene="mdcB"
                     /locus_tag="ACG816_000240"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4919160.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(46408..47484)
                     /locus_tag="ACG816_000241"
     CDS             complement(46408..47484)
                     /locus_tag="ACG816_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4919161.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(47508..48101)
                     /locus_tag="ACG816_000242"
     CDS             complement(47508..48101)
                     /locus_tag="ACG816_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4919162.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48105..49781)
                     /gene="mdcD"
                     /locus_tag="ACG816_000243"
     CDS             complement(48105..49781)
                     /gene="mdcD"
                     /locus_tag="ACG816_000243"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4919163.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(49771..50070)
                     /locus_tag="ACG816_000244"
     CDS             complement(49771..50070)
                     /locus_tag="ACG816_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4919164.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(50172..51818)
                     /gene="mdcA"
                     /locus_tag="ACG816_000245"
     CDS             complement(50172..51818)
                     /gene="mdcA"
                     /locus_tag="ACG816_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS4919165.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(51825..52736)
                     /locus_tag="ACG816_000246"
     CDS             complement(51825..52736)
                     /locus_tag="ACG816_000246"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4919166.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            52856..53713
                     /locus_tag="ACG816_000247"
     CDS             52856..53713
                     /locus_tag="ACG816_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4919167.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILHLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(53900..54262)
                     /locus_tag="ACG816_000248"
     CDS             complement(53900..54262)
                     /locus_tag="ACG816_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4919168.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(54451..54633)
                     /locus_tag="ACG816_000249"
     CDS             complement(54451..54633)
                     /locus_tag="ACG816_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919169.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            54608..56047
                     /locus_tag="ACG816_000250"
     CDS             54608..56047
                     /locus_tag="ACG816_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919170.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(56054..58606)
                     /locus_tag="ACG816_000251"
     CDS             complement(56054..58606)
                     /locus_tag="ACG816_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4919171.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(58720..60051)
                     /locus_tag="ACG816_000252"
     CDS             complement(58720..60051)
                     /locus_tag="ACG816_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4919172.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60017..61495)
                     /locus_tag="ACG816_000253"
     CDS             complement(60017..61495)
                     /locus_tag="ACG816_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4919173.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(61800..63635)
                     /gene="tdc"
                     /locus_tag="ACG816_000254"
     CDS             complement(61800..63635)
                     /gene="tdc"
                     /locus_tag="ACG816_000254"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4919174.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        18236 a        13706 c        10808 g        21116 t
ORIGIN      
        1 tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag
       61 aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt
      121 ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat
      181 tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag
      241 cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt
      301 tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag
      361 atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt
      421 tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa
      481 cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct agatcactga
      541 gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt
      601 gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct
      661 gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag
      721 aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga
      781 cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag
      841 gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat
      901 ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac
      961 gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga
     1021 atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag
     1081 ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg
     1141 ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag
     1201 tatacgtact attttcaacg gcgatatcat catctacgat cacactttct acttcaatat
     1261 cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct
     1321 taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat
     1381 ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta
     1441 caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt
     1501 ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca agttctggat
     1561 agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt
     1621 ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga
     1681 gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca
     1741 tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag
     1801 ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca
     1861 ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga
     1921 gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct
     1981 gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct
     2041 tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata
     2101 aatttctaat tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa
     2161 aagagaggag tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag
     2221 tagcttataa agggatcgtt tttggtgagg tcattactgg gatccatgca tttaaagact
     2281 tggaggcggg gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa
     2341 tgggtgcaag agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg
     2401 atgcgatcat tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga
     2461 tggtcacatg tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata
     2521 actaaagaga gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg
     2581 aggtactcta ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct
     2641 gctgcgacta acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt
     2701 gtttttggca aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct
     2761 gtcgtgcttc cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt
     2821 tggaaactag ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct
     2881 tcgctgatct ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt
     2941 agctttccag aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt
     3001 tggatcttcc cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc
     3061 tttttctcaa gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga
     3121 tcgattgctg cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt
     3181 tcttttcttt tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta
     3241 tttattgatg tatcttctga agatgaaatc gtttctcctg aattatctgt cggtacattg
     3301 gaagtcattt ctgtttccga ggcaccgttt tcagcaccag aagagcttat ggttgttgaa
     3361 ggttctgttt gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa taagaaaata
     3421 ctggaaacag caattggcca gattaacggc ttcattcttt ttttcatcac ttttcccatc
     3481 cttactactt tttaactaat ttcttattat aagcaaaaat gtttggaaga accataaaca
     3541 caacaggctg tttgtctaaa aaacggtgca tcttttattt ttttgtacgt tccgggcttt
     3601 tccatgtccc gcttccataa cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga
     3661 tcgttatcgc gttcactatc cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt
     3721 tcaattttct tcgtcgatca tctacaatca gcgaagctag caactggaaa aatcctgcga
     3781 ccatttcaaa gcagatgaac acaaaactga atgtaaaagt aatataaatc tgttcgtagt
     3841 ttccttgata tacataatgc ccgattaatc cagcaaaaag aactacggaa atacaaaaga
     3901 atattgacca gacaatactc aatgtttgat caatgaagat gatcgttctt ccaatatgtt
     3961 taaaaggatg gagcatcact tttttgaaat tcgtcagcca gacttctgtt ccaccttttg
     4021 cccaacgttt tctgtgtcga tatagcatct tcaacgtcac aggtacttcc atgaaaaaga
     4081 taatctcagg agcaaaaaca gataaccagt catttaactg atgatcccaa gcaatactga
     4141 tgtcttctgt cgctcgatct tgtctgaatc cgcccacatc gatcaaggct tcttttcgat
     4201 acatcgtatt tgctccactg tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt
     4261 taatgatgcc cacgatactt gagaattcaa ccgtttgaga ttttgcaatc aatttggtac
     4321 gattttggac atccatattt gcagtgaccg cagcaatatg tcttgcacca gggcgaatga
     4381 aataattcac atacctaatc agtgcatctg gctccggtac cgtgtctgca tcattgctta
     4441 aaatcaattt cccttttgca aaggcaagtc cgatattaaa cgcgtgggct tttcctttat
     4501 ttttttcgat ccgaaccaca cgaagattag cgtatttttt catcagtcta gctaatattt
     4561 ctggtgtttg gtccgttgaa ccatcgtctg tcactaatac ctcatagttg tgataattca
     4621 atttcgtcat caaatactcg attgtatatt cgatcacaat ttcttcatta tgggctggca
     4681 ccatgatcgt gatgaacggt tcgacactca agggaacatc tacccattct ttttgcttat
     4741 gtttgtataa aaacacataa caccagacac caataaacca tacaaatccg cctaaaatcg
     4801 gataagaaac gagaaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg
     4861 tatgggtgat tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa
     4921 tgtcgtatct agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc
     4981 ttttgttcca aaacgttccg tatacacttc ttcaagtaat tcttgtcttt ttttcaattt
     5041 ttcatcttca tattcaaaag atttatttac ttttttgtat agatttcgat tgttccacct
     5101 agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa
     5161 gaaatacagc attttctttt ctgataaaaa agcgtacaag gaaaaatcct tggctctttc
     5221 tggtctcgtt aatgggaaaa agacccatat aaaaggaaga acaacgatca tccatcctaa
     5281 aagagccact aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa aatacttatc
     5341 tttcaccaaa attgatgcaa cttcttctct tgttactttt ttcatatcct cacctcacgt
     5401 ttttttgtta cgacgtagga gtttaccata catttcattt cttttcacat aaagtgttcc
     5461 taaatttttc ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa aatattttgt
     5521 tctttttatc agttgttctt tttatttttt atctcttaat tatgttgctc tttccaatac
     5581 gacaatgctt ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc
     5641 atctccatcg ttttcaaatg gatataagat tacatgatca aattccagtc cttttgctag
     5701 actcaaagga atgatttggc agttttctaa tccatatgaa ttttttttaa tcgattcttg
     5761 tagcgtttgg gtttcctctt ctgtttgggt gataactgcc gctttttctc cttttagaga
     5821 aaccaaaatc tcagctagta ttttcaacaa ttcttcagag gatcgaaaag agcggtattc
     5881 gggttctttt ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat ccacagcata
     5941 ggttttgaaa agctctgtga ttgctccgct agaacggtaa ctatttctaa gatcataccg
     6001 atggatcggc agatggtagt cgtcgaaaca acgcataata tcagcaaaag tagtggaaga
     6061 attaaagatt gcctgattct cgtcacctac taaagtaaag tgagtttttg gaaataattc
     6121 gatcaataga cctaattgtg ccagggtata atcttgtact tcatcaatca atagatagcg
     6181 aaatgctggt acatttacct tttcaacaag taaatgacga atagctaata taatgactgc
     6241 ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt tttcataaat
     6301 tctttcaaac aactgatgct catccaccca tgccatttct tcgatttgtc tggttatttt
     6361 tcgatatttt ttttgtaaaa gtttccttgc ataagctaca atactttgtt ccgaatcatc
     6421 agaaatcaat ttaccgaata attccaactg cctatcttct gtcaacgaga gtacctgatt
     6481 ctgcacagca ggactttttg cttgtttcaa aagatgattc ttccattcgc tcatcagtgc
     6541 ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga gcaggaactt tctcatatat
     6601 tttttcgatc gtttttttag aaaggactgt ttttccattt ttagtaagtc cacggataaa
     6661 attcggatga tctaaaaaca aaggatctga ttgttttaga ttatcgataa actttttgct
     6721 acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg atacgtttga aataagcctc
     6781 ttcaccttct atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag
     6841 tggatttctt tcacctaatg atggtaggac attcgaaata tagtcgataa accgatgatt
     6901 aggtgataga atcagcaagt catcggatgt catcgtatct cgatataaat agagcagata
     6961 cgcaatacgc tgcatgatag cagaggtttt accacttcct gctacgccat ttaccaagat
     7021 attttgactt ttcacatccc gaatgattgt gttttgttcc gattggatcg tagcagtgat
     7081 agccttcatt tcattggtct catcttggcc taagacttct aataatacgt catcttgaat
     7141 agctacagac gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa attgtcgtct
     7201 ttctttaata accgtatcga ttttattttg acgaaccgta taagaagatg gaccaatcat
     7261 gttattgtaa aaaagttcgg caattggtga acgccaatca taaacaagat tttcatctgc
     7321 atcatctgtg aaatttgctg taccgatata aaaggattct tttggctcat tatccagaaa
     7381 gtctacaacg acttttccaa agtagggact tttcaataaa cgttcgatct ttgctagctg
     7441 attttctgcg tttttctgtt tgatattcat ctgatcgatt tctctatttt tcatttcaat
     7501 catcgaaaaa gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc
     7561 catttctttc aacgactggt ttccttctat ttttgccgta tccatcgatt cccgcagttc
     7621 gtttttttta tcaattagct tttgatacac gttcttcaga tatgcttctt ctaactgttt
     7681 ttgtgtgttg accatacaca ttctcctctt tttttaaagt ggtggaaccg attagtttac
     7741 ctcttttaag agtcgaatgc aagcttttta acgagtatca tttgttagga tatatcagtt
     7801 cttcaggtcg gacagtttga tataaagaaa atgcaccatc aaggtttttt acgtggtagc
     7861 cagactgctt caagattctt tccgctagat agctacgtaa tccgctatga cagctgacga
     7921 tatattcttg ttggctgtct aattcattta atcggtcccg aagttcattc aaagggatat
     7981 gtttggcttc cttgaatttg cccttttcca attcttctgc tgttcgcaca tctaataaaa
     8041 tttttccttt tgctagttct gtcggcaatt catgccattg tacggtttca ctaagccctt
     8101 ctgcaatgtt cattgctgca tatcctagca tgtttactgg atctttggct gagccaaatg
     8161 gcggtgcata cgtcaattct aattccggta aatcgaagat cgtcaaatgc cctttgattg
     8221 ctgttgctaa aatatcgatc cgtttatcca caccttttgc cccaacacct tgtgcaccat
     8281 aaatctcgcc agtctctgga tggaagatca acttcagtaa aatatctgtt gcttctggat
     8341 aatagcttgc atgatctttt cctgatacat gaatgacttc aaaagacagt tcgttttctt
     8401 tggctgttct ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt cgtataattg
     8461 ctgttccaat accgccttga tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc
     8521 taccttgacg attggctggt gaagcaagag aaataagtgc atcttttcca gtgatttgct
     8581 gttttacaac gatagcatcc cccaccgcat aaatatcagg aatattcgtt tgataacgtt
     8641 cgtctactag gatacccccg cgtatgccta attcgattcc agcggtcact gctagctggt
     8701 tatcaggacg aacaccaatg gataaaataa ctaaatcagc agggatggta cgcccatcat
     8761 ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac agcatgagaa gtaatcactt
     8821 ctactccttg atgagacaat tctttttcta taaaagctgc catttcttca tccagtgttg
     8881 gaagaatatg tggtgcttgt tcgatcacca tgacttctaa acctcttctt tttaggtttt
     8941 ctgccatttc caatccgatg aatcctgcac caacgatcac ggcacgcttt ggctgttttt
     9001 ctaatgcatg catcacttta tcgatatctg gtacatttct aatagaaaaa acattgtcgg
     9061 cttctgttat ccctggaatc gatggaacaa ctggttttgc accaggagat aaaaccaatg
     9121 catcgtactg ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt ttgttttcgg
     9181 gaaagatggc tgtcacttca tgatttggac gcacatctaa acaaaaacga gcggataatg
     9241 attctggcgt ttggacaaga agattttccc gttcactgat ttctcccgaa agataataag
     9301 gcaagccgca atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg atttctgcat
     9361 cttccattaa tcggcgaagc cgtgtagcag cagacattcc acctgctaca cctccgacaa
     9421 taacgatttt catcttattt ccctcctact gttcgatcaa accattgatt catgccacca
     9481 cgtacattca ctacatcata accagatttc tttaattctt tagctgcttg tttactcctc
     9541 atccctgatt gacaaatcac atagacggtc ttttctttgt ctccatcata tctgtttata
     9601 ctttgtaatg gaacattttt agctcctttt atatgcccgc cccggtattc tgcaggtgtt
     9661 ctaacatcaa gtaacgctgg attttccaaa agcttttcct ttaattctgg gactgttatc
     9721 gattttattg attgaaatag caaattcatt ttctgcacct catttacatt tttataaata
     9781 cctcatgggg tatattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt
     9841 tttttcacta ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa
     9901 aatagaggag aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt
     9961 acagagggac aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat
    10021 gtcatcactc aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt
    10081 gcagataatc tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct
    10141 aaactagaac aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt
    10201 cgttttccat attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct
    10261 gataaaagct tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg
    10321 ttatctagac tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg
    10381 cgccaatgct atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt
    10441 tgtaccagca ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa
    10501 gccttagtca gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt
    10561 tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc
    10621 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttgggcttat
    10681 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcggtttg
    10741 acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg ttttttgagc
    10801 taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt
    10861 agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg cataaatttg
    10921 gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt aaaatcagcg
    10981 gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt tgccgttttt
    11041 tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc
    11101 atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggttcggt
    11161 tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat
    11221 aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca gcatacaaga
    11281 caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc cacagcgttg
    11341 gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca gtcgctactt
    11401 tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt aatccagcta
    11461 caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc cacggtcgat
    11521 accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca
    11581 ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct agataatcag
    11641 acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc agcacaaaat
    11701 aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga atcgtttgta
    11761 tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc agcactcttt
    11821 tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt ttatttttct
    11881 ttacagacac tgatattatg agctaatttg agataatcac ttgctgtttt catcgtttac
    11941 tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag ctttttttga
    12001 tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca gactgtctaa
    12061 atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac gctgacgagg
    12121 cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta tatcttcata
    12181 aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg caaagagctg
    12241 acgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa attctttttt
    12301 ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca atttcatcga
    12361 catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctt ccaacccgca
    12421 actgatattt aggacgaagt gcttcccgat cgaaagccat agcagccggc acaaaatctt
    12481 catgatctcc ggcgaatttc ttgatttctc cataatgggt cgtggcaatg atcaatgccc
    12541 cttgctcata catagattcc atgattgcga tcgctagtgc agccccttca tttggctctg
    12601 ttccactgcc aatctcatct aaaagtacca acgtattcct ttttatcttt ttcaaaatag
    12661 cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc attttctaaa ttttggtggt
    12721 caccgacatc gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct tttgccggaa
    12781 cttgcatacc gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac acgactgttt
    12841 tgcctccggc atttgctccg gtgataatca agcctcgata gtctttaccg atttcaacat
    12901 ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt
    12961 ccgatttatt gattctaggt gtgcttccat tgatttctcg gctaaatttt gcccgagcaa
    13021 agatgatatc taacaccgtg atcgtttcga tcaataaatc gatcacttct tcgttttctg
    13081 cgatagcacc agtcaatgcc gccaggactt gatattcttc actgatttct tcagctttta
    13141 acaactgata gtgttcattc aatttagaaa tcacagttgg ttcaataaaa accgtcgttc
    13201 ttttatttga ttcatcgata atcgttccat ctattttatt tttatagctg gctttgatag
    13261 gaatcgtata gtattcgcct ttttgaacaa tcattgcttc ttggatcatt tctttgtttt
    13321 tcggatgacg caagaatttc agtaattttg actggatttc tttttcgatt agttgtaatt
    13381 gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact gactttatta ttttttattt
    13441 tctggtcaat caactcttct acatttatca aatcaggaag atgtttgcta taagcaaaca
    13501 acaagggtgt ctggtattga ttcttatcaa aaaactttgt gatcatccga ctgcttctaa
    13561 gaaaatccgc atattcgatc aaatcggttg gctgcagaat caaccctttt ttgacttgtt
    13621 ctgtcagtgc atcgatcctc ggcaacccca taaatggaac atgctgactg ctttctaaga
    13681 tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac agtttgtagg tttgtcgaga
    13741 ccgtcatatc gctgattcgt tttttgctat agtttccaat tgctcttgct tggatttcct
    13801 ttttgatccg atcaaattgt aattgactaa tcatttcgtt gtccatattc tctccttttc
    13861 ttgaagagat acctgtccca aaataaataa gggcctagag ataaataaaa ggatagaaca
    13921 cagcttaaaa aagtcatgtc ctatccttat tagaatacaa agatattgtt ttcctgacag
    13981 gtaccgtttc ataagcacac caaataaact ttgtttattg gctattcgac ttactgatta
    14041 gataccagct acactcacaa aacatacccc attcttctag gtcttattta ttttccactt
    14101 aactatagca agacacattt tggttgtcaa actttagtat gttttataga tttacaccat
    14161 taatgaatag acacctaccg cgataattgc tccgatcatt ggacctgcga ttggtaccca
    14221 tgaatatgcc caatctgaag ttcctttatt agctattggc aataccgcat gtgctaatcg
    14281 aggaccaaag tcccgagcag ggttgattgc atatcctgtt ggccctccta atgaaagacc
    14341 gatggctaag atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg
    14401 tccaaaagct agcaacccaa gaactaatac aaatgtacca attaattctg tgatcacatt
    14461 tgctggataa ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc
    14521 tttcgttgca ttccaatgtg gcaaataaga aagccaaaca agaatggccc ctgcaaaagc
    14581 tcctaaaact tgcgcaatga tgaaaggaac gacaagattc caactaattg cccctgtcat
    14641 cgccatagct agagagactg caggattcaa atgagctggg cccatgaatc cagaaatata
    14701 gacagccatt gttacagcga gtccccaacc aaatgcgatg acaatccagc cagaagcctg
    14761 tgctttactt ttgtttaaat tcacagccgc acacacacca tctccaagca aaactaggat
    14821 cagtgtccca ataaactccc ccatgatttg agtcatatca ctgttcattt atttctcccc
    14881 ctttagttcc cgcagatctg attctgcaat caatgcttct aaacgtttag tctgttgcgc
    14941 cttctcctct tctgtccatt caaaatatcc agcaattgca tcgatcacag gttgcttgat
    15001 ctcatcaagc tgatctcgtt caaacaacag atgatttgta cgacgaatga gataatcgcc
    15061 tggggccaaa accatttctt cttctaaacc ataacgtaac cgagctgact cagcgagact
    15121 caaaccagga taaggtgcca tttcttttgc taattcaaag atacgtcggg cattagtccc
    15181 gtaaaaatca gcgatataag tagcatcttc ttcctctaaa ccggctgcta ctccttcttt
    15241 tgctaattct gtcactgttt cttctaattt agttggatcg aagtttccac ctgaaatctg
    15301 atatttttta gagtcgattt ctttcgtctc tattccgtat tcttctttta ataattggcg
    15361 tatcaatcgc aaagctcctt ctgccatctt acggtaatcg gtgattttcc caccagataa
    15421 agtcagcaaa ccatctggtt ctctttctaa agaactacct ctggaaatcg tagaaggtgc
    15481 tttttcatca cggctgtttt ccaaatggtt caacacgtct tctacttcag cacgagaaac
    15541 tttgttttcc ttatattcac ttactgtatc gacaacttta ttgaagctct tgtctgaaat
    15601 cgatccatta tctccaccat tgtaatcaga accagaatta ccaatcaaca gtggacgaag
    15661 tcctgcccag cttgcttcga tatcattcaa tgtgatattt gcttctggat agcgatgatt
    15721 gatcacatcc aatagataat ctacatcttc ttgtgtgact ttcggatccg taaaatctcc
    15781 ttggtaatcc gtatctgtcg taccaaagta agtcttgttt tctcttggaa tagcaaaaac
    15841 catccgccca tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt
    15901 cgcatctaca actaaatgga tccctttagt tggacgcatt ttaggagaga ctggacgcgt
    15961 aaaattcaag tttcgtactt tatctaccca aggaccactc gtattgatca ctaattttgc
    16021 tttgatttcg atcacttcat ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc
    16081 gccatcatat aaaaagccaa ccgctttcat tttacttact agataagccc catcttcggc
    16141 agcctttttg atattatcaa tcactaaacg ggcatcattg ttgcggaaat ctagatagac
    16201 acccgcgcct tttagccctt cttttttcaa aaacggttct ctttccaata cttcttctgg
    16261 tgtcagcgta taattctcat atttggttcc tgtcacattt gccagtttgt cataaaggtc
    16321 cattgctact ttgaccgaga acatattgaa ggttgttgct ccttcgccct catagattgg
    16381 caaaagcatt ggatctggtt ttgggatgtg tggggcaata ctttgtacga ctgcacgttc
    16441 accaactgtg tcagctacta cttccacatc aaatgtcttc agataacgaa taccgccatg
    16501 aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc
    16561 tgttttgatt cctgatgctg ctgactggac tgccacaccg gcaccagtga ttccgccacc
    16621 gatgatcaac agatccaatt cttcttgctg cattttttga atgctatctt gtcttgtctt
    16681 gtttgaaaac atatggattt cctccttatt caaatgcttg ggtagctttt actgcttttt
    16741 tccaaccgtt gtaaagtttt tctcggcgct cttcctccat ttgaacttca aatagcttgc
    16801 cagcttcata aaattcgcgg atctcgtctg tatctttcca ataaccgaca gctaatcctg
    16861 ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc tgctcgttgg atcggtacat
    16921 tcaagatatc tgtttggaat tgcatcagat attcattgtt tgctgcaccg ccatctactt
    16981 tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt atcgatgata tctctcactt
    17041 gataagcaat cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca
    17101 agccaaacat agcccctcgt gctgcctgat cccaataagg agcacctagc ccaacgaatg
    17161 caggaacaac ataaacctca tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc
    17221 tagctttttg caacatttga agtccatctc tcagccattg aatcgaagaa ccagccacaa
    17281 aaatacttcc ttctaaagca tagtaaacct ttccatttat gccgtaacca attgtcgtca
    17341 ataaattatt tttggataat tgcggttttt caccggtatt catgacaata aatgaacctg
    17401 taccataagt attcttgacc atgcctggtt cgaatgccat ttgtccgaat aatgcggctt
    17461 gctggtctcc tgccatccct gaaattggtg cttcgcttcc ataaaaatga taaccttgcg
    17521 tataaccata aacttctgag ttagaagtga cttttggtaa catcactcga ggaatattta
    17581 gtaaatctaa aatctcctga tcccaatcta gctgatggat attgaacaac atcgtccgac
    17641 tggcattcga gtaatcagta acatgtgctt gtccatcggt cagtttccat actaaccaag
    17701 aatcgatcgt tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg ccttctacat
    17761 gatcaagaat ccagcgtatc ttcgttgctg agaaataggc atcgatcact aaccctgttt
    17821 tttcgtggaa aaattcttga tagccttcat tttttaagtt atctgcaatt ccagctgact
    17881 gtctggattg ccaaacaact gcatgataga ttggacgtcc tgtatctttt tcccatatta
    17941 ctgttgtttc acgctggttc gttatgccaa tcccagcaat ctgatttggt ttgatccctg
    18001 attcgatgaa cgctccggca ataactgact gtacagaatt ccagatttca tttgcatcat
    18061 gttccaccca actttcttgc gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt
    18121 gtcttccttt cttgtcaaag ataattgctc gtgagctagt tgtaccttgg tcaatcgcca
    18181 tgatatacgt tgattctgtc attgtgattc cctccattat atgcaaacgc tttctttaac
    18241 tactcttatc atactactgt tttaaaaaaa gaaactgtca aaattttatt ctttttttct
    18301 ttttttgaca gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg
    18361 tctctattct tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat
    18421 attcacactg ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt
    18481 cttcataact tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt
    18541 gaaaagcaat acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg
    18601 caatataaaa atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct
    18661 cactatcttc agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca
    18721 gtaactgtgt taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct
    18781 catagacttc gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt
    18841 agcctactgc cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg
    18901 tttcccgaat gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat
    18961 actgataaaa tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca
    19021 ttgtttcgta tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat
    19081 cgattgcttg ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt
    19141 acgtactttt ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg
    19201 cttctgctga tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc
    19261 ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata
    19321 gttcataata aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt
    19381 gtaatccaaa ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact
    19441 cactgatatt aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat
    19501 agacgagcat cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac
    19561 ttacatcttc gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt
    19621 tttccatcat tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg
    19681 acagttggac atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt
    19741 ttcgcaaaat caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag
    19801 gcagtattcg ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat
    19861 agctcatgtt cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg
    19921 tcaagaaggt ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca
    19981 gaatcaaata gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct
    20041 acgtatcctg tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg
    20101 cttcctaaga aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt
    20161 gtctgtgagc cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact
    20221 agagaaacat acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc
    20281 gcataaacgt ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca
    20341 ttcaatgtta attgttcttc ccacatctga ttgtcgggac gagaatgatt agccagcaaa
    20401 acaagatcat agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc
    20461 aaagacacaa cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact
    20521 aacggttctg tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa
    20581 agatggattt gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct
    20641 gcaccaataa ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt
    20701 tgtatctttt taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga
    20761 tttgaaccag tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg
    20821 actttttttt ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg
    20881 tcagtcagtt cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt
    20941 aatatcaaat tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat
    21001 atttcacttg ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac
    21061 ccgccgacaa ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa
    21121 cttctcaatc agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat
    21181 gcagcagaat caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact
    21241 agtggaccta attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata
    21301 aaagaaaaaa actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa
    21361 agacgagttt tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt
    21421 ctttaatgct tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt
    21481 aagaagccgc ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc
    21541 tcctttttct ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc
    21601 ttcctcttct tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga
    21661 ctcatctgta actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc
    21721 atttcgatag atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat
    21781 tgttccgtgg ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg
    21841 acgtaaggta tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc
    21901 gatttcatcc atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact
    21961 gacaaaatct gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact
    22021 taaccacaaa cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat
    22081 caatactttc attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc
    22141 aagaaattat ataatgggct tggcaaataa agctctttat atgcttcttc aaaaggtttc
    22201 gttacaaatt catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc
    22261 atttgctgtc caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga
    22321 ttgcggatcc caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact
    22381 tcaagaatcg atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat
    22441 gaaacatgca gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct
    22501 tcaaaacgga tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat
    22561 ggttttttag catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa
    22621 cgataacgat tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag
    22681 aaaaattgtt ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat
    22741 tcaatcgctc tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca
    22801 ctcattgctc gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca
    22861 ggctgttctc cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata
    22921 atcgcttttg cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt
    22981 tcatcatgag cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca
    23041 gcaccagtaa taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct
    23101 tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg
    23161 ttcttcaatt gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt
    23221 cctcccataa ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt
    23281 agttgtacat gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc
    23341 atcattcatt gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga
    23401 ctgatgaagt tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt
    23461 aagataaact aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa
    23521 tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt
    23581 ttgtggtatt ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag
    23641 acctgtcctt ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac
    23701 gctaaagaaa agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc
    23761 attggcgtac atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc
    23821 gcctcctctg tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt
    23881 ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt
    23941 tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta
    24001 ataccgaaaa actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg
    24061 catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc
    24121 taatgccgat ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga
    24181 actccgagtc acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga
    24241 cttgtgatcg aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa
    24301 gacttttgtc acagtccctt atttcctttt attataggat ttcgccattt gattggatga
    24361 cttgtctgta ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt
    24421 catcttgatc aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga
    24481 cgatatcaat gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt
    24541 ccttcatctg ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc
    24601 catcttcttc tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt
    24661 gatacctatc atataattga ttcaaagcaa ttctcaaacc tttaggatca atctgccagc
    24721 cccattcact tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga
    24781 ccccttccca attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa
    24841 caaaatcaac agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga
    24901 cacctttttc ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat
    24961 cactaaagaa caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac
    25021 aagaatacgg aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa
    25081 tttcatgaca agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt
    25141 atttcgcttg ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt
    25201 gatgttttac acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc
    25261 gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc
    25321 caccatattc tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct
    25381 caatcccata ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat
    25441 tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc
    25501 atttaaaacc catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat
    25561 ttccgtgtct tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct
    25621 gatgcgtcat catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg
    25681 tatacacatc agacacagat aatccttttt catctacatc ccatgcacct tcacattgat
    25741 tggccgctgt cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc
    25801 taaacttgct agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa
    25861 tctactacta tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc
    25921 ttgcaaatct acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc
    25981 actttgaaag tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc
    26041 tgctccttct tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc
    26101 cccgtttaca gaactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa
    26161 agatccacta gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc
    26221 aggagtactt aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa
    26281 aagaaaagtt agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac
    26341 aaatccttca ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc
    26401 accggctcct gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta
    26461 aaatggtcgt ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa
    26521 tgctgtgata aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa
    26581 taccccaagt actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt
    26641 atcacgaccg tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc
    26701 aaaaatcact aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa
    26761 gttataaaca gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc
    26821 tacaactaat aaaataattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt
    26881 tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac
    26941 agctaaaatt gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag
    27001 aaatcgcaag gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc
    27061 tgcaataacc atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa
    27121 aaatataggc aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc
    27181 tgattcagta gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc
    27241 acttcctgcc aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga
    27301 gtttaaaatg ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt
    27361 catttgtttg gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa
    27421 cattcctcca gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc
    27481 tgccttctta ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct
    27541 gacattttct tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat
    27601 ttttacttcc cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt
    27661 cttagaaaac ggtttctcca ttcaattagt accccttcta atagcgaacg aaactgtaca
    27721 atagtgtgta tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat
    27781 atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg
    27841 tataagtggt atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag
    27901 acagcacaac aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat
    27961 tagttgctga atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta
    28021 atgatcaagc agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat
    28081 ctgataaatc tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc
    28141 ccactcaaaa ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt
    28201 tgattgattt ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat
    28261 actttgttga atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat
    28321 cagtatctcc ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata
    28381 agcgctttca gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa
    28441 ataaatttct aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg
    28501 tccggcatcc atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg
    28561 gaacaataac gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag
    28621 attcttctct atttctttgt ttttttcatc attaatcatc aaataattta actctaatag
    28681 tcgattttca attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa
    28741 cttttttgtg agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa
    28801 aagttcttct cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc
    28861 tcctgaaaca atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg
    28921 catgatattg gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt
    28981 ttctgttcca acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag
    29041 tccttttgtc gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat
    29101 cgtttttatt tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg
    29161 catactgaca tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc
    29221 catgctggaa tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga
    29281 ttcaattgat tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc
    29341 gtagatagca agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc
    29401 ttcagctaat tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg
    29461 gagttgtcgt ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga
    29521 aaaacctctc aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc
    29581 tatattcaag gtttttacca ttagactgga taatttgttt ataatattca aaactatctt
    29641 ttttcaatct cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat
    29701 atcgcttcct catttcccct gttcctgccg atacgatatc gattggtccc caccataagt
    29761 aaccaatgat ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta
    29821 gatactcaca acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc
    29881 caatgccatt ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga
    29941 cataacgaaa ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca
    30001 aaaagggatt atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt
    30061 tatagacagc gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc
    30121 actgttcgtc tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa
    30181 tataagctgg ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa
    30241 attgcagtga accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag
    30301 aaaagctaca cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag
    30361 cacgggcgtt cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg
    30421 tagcaatggg ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag
    30481 gcatacgaaa agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt
    30541 agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc
    30601 accccccata ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg
    30661 gctcgatatc atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct
    30721 catttggctt ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa
    30781 aagcttttaa acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat
    30841 caatcgcttc atgagaaaga taatggagtg attcgtctaa agcaatctcc caaatctttt
    30901 tttcttcatt ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca
    30961 cattaggtaa tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc
    31021 cgccccataa aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc
    31081 aacaatttct tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg
    31141 gcagaagaga agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa
    31201 tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt
    31261 acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc
    31321 agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt
    31381 tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca
    31441 aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt
    31501 tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac
    31561 gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg aattccagta
    31621 gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct
    31681 ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta
    31741 tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc
    31801 atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata
    31861 gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt
    31921 cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt
    31981 tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg
    32041 tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca
    32101 tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca
    32161 acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc
    32221 agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg
    32281 tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct
    32341 tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga
    32401 aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat
    32461 ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt
    32521 tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc
    32581 agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct
    32641 tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata cccattgtca
    32701 tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga
    32761 taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga
    32821 aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt
    32881 ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac
    32941 taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca
    33001 aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa
    33061 gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag
    33121 ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc
    33181 agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat
    33241 acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg
    33301 gtaattggat agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc
    33361 atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt
    33421 cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa
    33481 gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta
    33541 atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca
    33601 ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa
    33661 gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca
    33721 actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac
    33781 atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa
    33841 cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa
    33901 atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata
    33961 gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat
    34021 cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg
    34081 ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg
    34141 atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg
    34201 ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa
    34261 gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa
    34321 acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca
    34381 gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta
    34441 aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa
    34501 agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta
    34561 aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta
    34621 aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc
    34681 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
    34741 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc
    34801 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
    34861 atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
    34921 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
    34981 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
    35041 aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc
    35101 ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag
    35161 gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta
    35221 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
    35281 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
    35341 tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt
    35401 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
    35461 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
    35521 ttacaaaaaa aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg
    35581 cttctgattc atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga
    35641 ttttttcctt caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg
    35701 attcaatgac cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac
    35761 gccagttgag acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa
    35821 gtgccggagc atcggaatct gcacctaaag gcaggaatga ttcgccacct tgggcttttc
    35881 ctaaatgcag atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag
    35941 acaaggaaat actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt
    36001 gatttgcacg tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg
    36061 tggtaaatgc ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa
    36121 ggtaatttct tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt
    36181 cttttgttac gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa
    36241 atacttccca gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta
    36301 gaataattgc ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg
    36361 ttcgctgtat ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc
    36421 ccaaacaccg agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc
    36481 tagtaaaaat gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat
    36541 ttctaaaaaa tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta
    36601 acccgcaggc tcttgatcga tactcactag gaaaaaatcg gtttctttat cttttagctc
    36661 ttctgtaagt ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtga
    36721 attatctttt ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta gtaagtctac
    36781 atctgaaggc gtcatttttc gaatttcgta tttattcacg gcttcttcct ttcaatagat
    36841 ccttttattg ccttttttga caaaattcca atcttcgcta acattcttgc atactttatt
    36901 taaaagatat tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt
    36961 ggagtagttg ttttctcgaa tgatgaaagg atagatttct gctccttttt tagtcggata
    37021 gagtttcttg atttttttat tttctttatc ttccaaacgt tcgatcatcc cattggattc
    37081 taatttcttg attgctcggg cagtcgttgt acgatctact ttgatcattt ctgctagttt
    37141 ttcttggatg attccaggat tttcgcaaat acgtactaaa taaagatact gtcctcttgt
    37201 taaatcgact tctttgaatt cgatattgct aatagaatct agcgctcggg caatcattcc
    37261 gatgtctcga aggatttcag ccatttcatc ccacctttat tttgtttgta agactagttc
    37321 acctcttttt gttgcaaatg caactatttt caataaaaaa atcaagttct tttttaataa
    37381 ctagtaaagt atattctttt cttcttttac gaaaaagctc aatcacaata gatctggttt
    37441 ttgttttcta aaataagtaa ctgtttcttc gttgttcgct tttctgatag ctttggcaac
    37501 tcttctatac taaaaaatcc catgttggaa gtttcattat tctcaataaa cttggcctct
    37561 tcaccgtgga tcgcacaagc aaaaacaagt ttataatact gaaaaagctg tggaatatct
    37621 ttttgtttat tcgtatcaaa aactgctcgc aactgagtag tttctactgt taatcctgtt
    37681 tcttcataga cttctttacg cacattttct tcaggagaca atcctatttc agcaaatcca
    37741 ccaggaagtg accattcttt cgtccgttta tcttcgatta gtaggacctt gccatctttt
    37801 ttgatatagg cacgaacatc tacttttggt gttggatgtc cttctccaat cgttgtgagc
    37861 gcttttattt cttcgaaagt ttcttttcct atttcactga tcagttccaa tgaaatcgtc
    37921 cggatctcct caaaacgctc tttatcaaat acatcttttc cataatatag accagcttga
    37981 gaaagtgcga gtagtctttt gtattgagcc aacttcttgt tcattctctt cccttctttt
    38041 ttagcaaatg tatgactatg taatcaagta tagaagactg gtttaattac ggcaaataaa
    38101 tataatgaaa tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa aagacattcg
    38161 acttctctgg ggctgtgaat aaatggcgtt caaaagcttg catctgcggt aataagcccc
    38221 accaaacgaa aaatatgacg agctattctt gtctggctgt tcttattaat tgaggcagga
    38281 cagctttttc acaacctccc cactaaacgg tgttctccta ccaactcctc ggtcacaagt
    38341 cacaatattc atcaaacatg agaagctgtt tttttgaaat tgctccttgt gactcggagc
    38401 tgaacgacag gctcacgacc tctcggtaaa cagcgttcaa aagccaattt ttcgatatta
    38461 agccatttct ttgttactta ttgtcattga tttctttttc taatttatcc aatagtggag
    38521 tgtaatccac ttgagtatag tctgttacat caaattcata tagtgttcct aactggaatt
    38581 tgtcatattt tcggtcggcg atgatctttt tgaatgcttc tttcgtaata tgattggtcc
    38641 ctttcgttcg atcttccaat acgtcaccat agtgataatg atccatgata tagctcaaat
    38701 gacgatctga ttcacgatca cgtttgtacc gacgttgcca taacacttca aagtctgctg
    38761 ttaggcgaat cgtgatcacc tgatatccgt atctatctgc taattcttgg agtcgtgctt
    38821 tttgcttatc actaaaagga tactcggaca caatcacttt ctttcctgct tccatataca
    38881 aatcaagtgc tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt
    38941 ccaaattgtt aaacccgatg gattctgcat agtattcctt gatttcatcg agcgtgatgg
    39001 catacatatc tggaaatcgt tcccgtagtt tattcataag atatgttttt cctgtaccag
    39061 gactaccagc cagaagaatg aaatactttt tcatctcatt tatttcctaa gttaattgga
    39121 ctttccaagt tcataaacag tccaatctta tgcttgacaa tttcttttac tgcttcattt
    39181 gctggaatca cattatggcg aagagacttg ttgatttctg tttcactaaa ggtttcttta
    39241 gctactttcg tccagttgac taaaagctcc gttccaacat tgaatttgcg gatcccgtta
    39301 ttgatcaatt caggataatc ttcttctttc acacctgttc ctccatggat aactaggggg
    39361 acatcaacta ctgcatcgat ttctttcagc aagggaatgt ttacttctgt ttttgatttg
    39421 aattgtccat gattcgtacc gatagcaata gctaatgcat ccacgcctgt agcttctaca
    39481 aatttcactg cgtcttccgg tcttgtatat accttgtcat tttcagcgac atggattcct
    39541 tcttctgtac cgccgattgt tccaagttct ccttctacgg aaacaccgtg cgcatgggca
    39601 tattctacta ctgcttttgt cttcaggata ttttctttga aaggcagatg ggacccatca
    39661 tacatgacag aagtatatcc gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg
    39721 tggtctaagt gcaagactgc atcaaccatt tcttcttctg caatagctgt cattacattg
    39781 actaacacat gcatgccgat atatttagcg gtatcgacac ttgtttgaat aatgatcggc
    39841 gaacccattt cttttgctgc gcctagcata gcaggaagca tttctaggtt atgcgtatta
    39901 aaagctccaa ctgtaaaatt caactcttct gctgtttttg ttacttcttt caatgttgta
    39961 tacatctttt attgccacct tatcttttta tttgtttcct ttgatcgtta cttgttttgc
    40021 aatttgtcgc atctcatcca ttttgcccat gtagtaatcg atcccttgat catcaccagc
    40081 acgagcagca tctgcacgct tctggttata gaggctcatc agttttttat acccatctcc
    40141 gatcgtctgt ttattttcca aactcttttc taaatattgg atggcttgat cgttcacata
    40201 caattcagaa aatttaagtc caacttgttc ataaagtttc gctaatttat gcttatcttg
    40261 ggtagtggaa atctcttgtt tcaattgtgt aatctctgat tgaatctttt cggtcgcttc
    40321 tggcgatagg gctttttctt cttttgccag aacttttttc ggttcttttt cttttttctt
    40381 aaaaaatcca aacatcattt tccctacttt caattaaaat gctttcaaaa ctgggcttaa
    40441 gatccaggca tataagagtg ctgcggctac tgtatctaca tcggttgctg tagcattgtt
    40501 gaatcccatc gaattaaaga tcgttactag caaagcgggc aataatgtaa taaagaaacc
    40561 atgagcaata ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc caaatatccc
    40621 tgctgtccct ccggcaaaga aattggttag cataccaggt aaaatcatcg ctaagccgaa
    40681 catgggcaaa gtaaacatgg caataatcgt accaatcgtt gtcgtaatga aacctaaaat
    40741 gacagcattt gggctataag gaaagaaaac tggacaatct aaggcaggga tcgcatcagg
    40801 aactaatttc atagctatcc ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac
    40861 accagctagc aatacataca cacccactac gaattggatg gcttgtaaaa aggcaaacat
    40921 gatatagttt tgatctcctg ataattcaga agcgaatggt tcgcctgcaa ataaaacagt
    40981 gatgatatat agcgggacca tcacgaccat gacagacaaa taagtatctt gcagaaattc
    41041 gaaatgtgtc ggcaacttga tatcttcgat cgacttttta ttttcgcctt tctcaccaaa
    41101 aagcttagca acaccggctt caaataagta cccgatcgta caaaaatgac ctaaagcaat
    41161 atcatttgat ccagtgattt tacgaatgat cggttgcgct actgccggca tagcaatggc
    41221 gaaacaagct ccgataaaac caccgaccaa gatcaatacg atccctcgca gaccggcaaa
    41281 atagccaaag actgtcgtca tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat
    41341 gtacttccat tttgtgaaac gagccaaaat aatattgaat acaaaaatgg ctaagaacgt
    41401 cagtgcaata tctctaccta aacccaaatc attcattgct tgaccattga tagattcaat
    41461 agaagggatg atcccttgca tatgaaatcc ttcagtaaag attttcccga aataagttaa
    41521 acttccaacg atgatgctcg aaccggcact caacacttga aatcctagga gtgtctttaa
    41581 cgtgccagag atgacttgtc ctgctgattt cttctgcagt agtagaccca acatagcaat
    41641 caatgcaatg gtgatcgatg cctgagtcaa aatattttcg atgataaaat tgataacgcc
    41701 catgttttta ccctcttttc tttttgtatt tagataactc ccttatcttg cagaactggg
    41761 gtgatttttt cttcaatttc attttttgaa acaatatttt tcaaatagat catcgccgtt
    41821 ttaggatcaa ctgcgaactt ttcgaactgc gaacgaaaat tttctgctgt gatgatcaca
    41881 tctggtttca tggaaactgc ggaagaaata tcacaatggt ccagtttggc ttctactcct
    41941 aatttgttca aaacatcctc agcagacatc tgtgctgcaa aactacttcc taaacctgca
    42001 ccgcatacga ataaaatgtt caatttgttc atctttgttc tcccccgtat catttattat
    42061 ttctcaacta ctgaaaaaag cagttgtttg aaatcttcga tatttgtgca ttcaatcaat
    42121 cgatccaact tctcttcgtt gttgatcaat tcaattagtt ctttcatgat gttcagatgc
    42181 gagtaggagt ctactgctgc caaacaaaaa ataatcttta ctggatccat ttcaggatta
    42241 ccgaaatcga ttggttgctc aatcgtagct acgctgactc ctaatttatt gaccccatct
    42301 tctggtcggg catgggctag tgccatgtgt ttgccaatca cgatatacgg tccgtaatca
    42361 ttcactgcac gaatcatcgc ttcgacatag cgctcttcga caatgttttc tttaaccaat
    42421 ggtctagcag ctagttcaat cgattcctcc caagtagtgg ctttttcttt gatcatgata
    42481 ttgctgtccg tcaaaatatc ttttaacata ggctggatct cccttttgtt gatttctaaa
    42541 ttgtttgttt cgaataattt ttctaacttg ccatagatga ttcgatctac tcggccacca
    42601 ctttctttca tgacatctat tatttgagtg aataactccg tcgattcatg actgttaggc
    42661 tctaaccgct gtagttcttc atttcttttc aaaaagtctt ctataaccaa gatattcgtc
    42721 tcagttaaaa tcggatcgac aactaaaagc ggcttatttc gataattcaa ttgaaaggtg
    42781 ctgaaaacca gattgacatc caatttatct actaattcga tttcttttga acttaaaact
    42841 gcagtaatct cgatctgagg aaacttttct ttcagattct ctgctaacag atttccagta
    42901 gccatcccat ggttacagac aacgactgat ttataaacat aattcaagtc tcgcttgatt
    42961 acactggcga tcgtcgaaaa gtggatgaca agaaaagcta tctcgtcctc cagtatcttg
    43021 ccaccaatta cttcttcgat cgtaggaaca aaatgagtga ctgcgcggta gattgggaaa
    43081 taatttgttc ggatgttttc tttcaatgga ttcatgactt ggatatgatt cttgattctt
    43141 acaatcagtg cagccatgtg cttatagaga ttttcgaata aagcttcttc ctttagatgg
    43201 aaaggtacgt gtgtctcgtt ctctacaaat tggatcaatt ggatcgttag taattgagct
    43261 tgtacccact cgattgaatt ggagatatct ctagcattga acgtctcaat agtgaaacga
    43321 agataatttc tttctacttg cgggacattg tccaattgaa attcctcgat catgtggtca
    43381 ataaatgcag aaaagctact gtcttttatt tctgtatttt tccaattaat ggcagagata
    43441 aattcatgtt tcctgattcg ctggatccag atcaatgtaa aaagcaggat ttgtttttta
    43501 taaatatcat cttcttgttt caacaactta ttggaataga tctcctgtat ttttttgatt
    43561 tccaccaaag gaaaataatg gagaaagatt ttttgagccg gtgttaattg tgcatcttga
    43621 ttcataaaga agtcggtcat tcccagattc tgattgataa aatcatagat catcgtacgg
    43681 atagatcgtt ctgaaccttt ataaaccagt ccttgctttc cataactgac gatctctatc
    43741 ccataactga ccagtgttgc tcgtaaccgt ctcatatctt catctaatgt acttttagag
    43801 atttgaaatt cttcttcttt tttgtttaga tggatcggga ttcggctgta agcaacggct
    43861 aaaactagat caaaaatacg ttcgcgccga ttcaaaaagc cgtctttcac atcttttatt
    43921 tcagtttcta atcgagcttg cttttccttt tccagcttca acaaaaaacc tttgttgcga
    43981 attgtacgga tctccggaag ctttttactg ctcaaccaga tattcgcttc aagaacttcc
    44041 tgacggatcg tccgctgact gacattgaac tcacttgcta gatgttttgc ttttactgga
    44101 tattgggctg tcatgattct gtgaagtatt tcaattgttc ttgttttcat agaaatgatc
    44161 tccttctgat ttcaagtata tggttccgct tacataataa aaagtagata tttctgccac
    44221 ttgaagtgga aagaaagtaa gcaaagcaaa caaaaaataa gccatatgct ttttcatctc
    44281 ctcaacgaaa aagacatacg cctattatta caaaaatttt gcccatctat acaaaaaaga
    44341 ggttgtgaaa aagctgtcct gcatcaagta ataagaacaa ctaaactaaa atagtgcttc
    44401 ctatttttcg tttggttgtt cttattatcg cagatgcaag cttttaaaca ccatttagtg
    44461 gagaggttgt gacagagatg ttcagccttg agcgataaga agcgattttg aaaaatcgct
    44521 tcccatattt tacaaaatcg tgccttattg ccgataggct atctctggca caccgtttat
    44581 tcagaaaaag gagctgcgac aaggcttttg tcacaactcc gttcataaat atttcctgtc
    44641 aatccagcat aatcggctga ataaaatcgg cttgccatct tttttctttc tcagaaaaat
    44701 caaaatacca tatctgagaa aaatactgtc gttgtgtctt acttcctgag atcaatcgcc
    44761 catcttttcg atcgactgaa taatcacagc atctgaaatc aattcggatg gcaaaactat
    44821 tttcgtggat ctgtcgataa tttcctactt cctgaatagt tattttttta gtatgatttc
    44881 ttacaccgtc tgctttgttt ttttgcagaa ctttctgatg ttcggcaaac agcttgtctg
    44941 tataacagct tctggctttt tcaggagttt cttcttccca agcggattgg atcttcatga
    45001 atatcttttg gatttccaga aactgtgctt tcttttcttt agccgttcct gacatgcggg
    45061 acaatatctc cgctttcagc cttttctttt gtcttttatt cttgacgata gaaaaagcag
    45121 aaacccctcc tactgacaaa acagctaatg caagaaatcc cgtttgctca taactagaag
    45181 gacctgtacc tggaccataa tacccataag aatctctgcc gtaagaacga ctgccaaaat
    45241 aactgccccc atatgaacga ccaccaaatg aacctctgcc cgaacttcga ccgctagatg
    45301 tacctccacc atgaccaccc gcgacagcga atccttgggt cggcagagca aacaaacata
    45361 ggcccactaa caaaatgatt gctaaataat tgattttttt cattgtcttc ctcgttatct
    45421 tttattacaa aaaactattt ttatgatagc cttgctaaaa ggaagagtca ataaccagat
    45481 gcataaactg tttttttagc aattttgtct tatttagtca ttgagtaaca tcggacaaat
    45541 gtatgtgtcg gcagtccata attcctctat cttcagcatg aacaagcttg cagacaagag
    45601 atctgcgctt cctccaggag agatttggtt ttcctgacaa aagtgcaaca gtgctgtaaa
    45661 tggacgattt ggtaaagcac tttgactagc agcatgagca agtttttgca tttccactaa
    45721 cgttttctga tcactgcgat gcaatacgca tgtgtcatct agcgaactca tcaaaactaa
    45781 caacatatgc atttgtttct gtacatatga atcattatct agctccttag ctgcttgaat
    45841 tgcaagcctg atatgcggat accctaatat cgcttcttca tatgcaccat ttacgccata
    45901 tttttgcttc accctatgac catgagtggt ttttttatga cgatattttg tatcaggaaa
    45961 agcagcaagc ttgctgacat catcaagtat tttcgataaa gaaaaaggct gttccatact
    46021 gattttttgt gcaatgacac tgatcaatag ccccattgcc caaatcgctc ctttatgggt
    46081 attgatgcca tttgtagcaa tcagcattgc cttttccgct tttcgcccaa tctcagcaat
    46141 tctttctctt aactcctgat caatagcatg atgccatgct atttgagcca tttcttcaaa
    46201 aaaaggagtt agcgtcttag cagacttaag aaataaagca tagtccatat cggaatgtgc
    46261 acccgcatcc agtgcatcca ctaatccagg ttttggactt aatgttactt ctgcgatcaa
    46321 tgcttcctct accaacgcag cgagttgctg agtcactgct ttttttcgtt cgtcatttat
    46381 catgacactc actccttctt cctactatta cttgctgaaa ccttttttga tagctaccca
    46441 aatgatatga agtaaccagc ttaaagcaaa tgcacaactt gccgtcatta gataagctac
    46501 aagaatcatg actcctgaag gtaactgcag ccaactatct ttaaacaata ataacagtac
    46561 ttggaaaaca aatacattgg ctaaatacgt acgataagca taaacagaca accatttgaa
    46621 caatggcaat tttggcgaat cgtttacaat cattttggaa gcgcctatga agacagcaaa
    46681 gatgatgact aaactgtata aactttggat cgtattcaag taaggtgcat tcgcaaagct
    46741 caagtctcct ggatgtgcga gcaatgaacg gacagaaaag aacgcgataa ctaaggcaac
    46801 aggtaagaaa agataacgta ctctgtccaa atattgatag actttttcgt gataaacaaa
    46861 tgcggctacc ccataaatca tgaaactagc aacaaaccga tctaataagt accagctgtc
    46921 atgataaggc ccagtcaata cttgtgtttg atagaacaga taccatgcga catataaaat
    46981 cgttgaacca atcaatactg gccatacata ttttttatta gcaaatactt tgtaaccgag
    47041 ccaaacaaaa aacggcatga acaattggat ctgcagcatc ataacggtat accataaatg
    47101 aggcgctcca tcacctgtga catatttcag taaaaagctt gcagtatccg tatacggagt
    47161 ttgttgttgg actgctggaa atagccaaag ataagccgtc gtccatagaa gatacggaac
    47221 gactagttca aaaaactttt ctttcaaaaa ttcccaataa gacaaatgct gacttgtttt
    47281 taccatatta taaacaattg caaaaataaa cattggtgct gtgtatttaa cgatgacgta
    47341 aaaggtacca agaaaagctg tatttgttgc gctgtggctg tttgccaaag caaagctcaa
    47401 tatcgtttgc atcataacag ccgttacagc tgatactttc tggaagtcac ttacgttgat
    47461 tttgattttt ttgttttcaa acatctcttt ctctctcttt ttctttatta ccaaagttct
    47521 ttggttaatt taggtccttc gactgttttc aatagaattt ttttctctgg ctctctcaaa
    47581 tactctttta gtgaaaaacc gccttgccga gtcactactt gggtatcgaa ttgctcaaaa
    47641 aaagcaggat gcgattgaat tacctgaatt ggcaattcaa tcggtgaatt tgcgtacagc
    47701 agcaaatcaa aatcactggt ttcttttact gccggaatcc cagtcgataa ttcaaatcct
    47761 aagctgccgc cgattcccca tttacatcct gataatatgt ttctagcagc atgaaattgc
    47821 tgataaacag gataatccat gattttctct tgtctatacg attcttgttc gatgatgtcc
    47881 cacggatgaa tgacttgttt gacggcatgt tttgagattt ctgttgacca tctctgattg
    47941 cgggcaggtc cacgtaaacc aatcggtagt atttctgcgt gatccgtcga cgcacgacga
    48001 actgttccga acatcttttc agttagaacg acccattcag gcaaggtgaa cccttctatt
    48061 tcatgtttat caaaaaggac aatatcatgt gcttgaatca ttttttatgc atcccattct
    48121 tcgtccattt tggcacggac tttatttgtt tcgacacgtc cgaatgtttt ggctgtttcg
    48181 tttgtgtatc gatttgtcag catcgtgtca tttgtattgc ggacatcttg aatggcttca
    48241 ttgattgctt cgatgacttt gctgacattt tgttctgaag cagcttggtc ggtgatatct
    48301 tctaggaaat gatagatcgc tcctagtttt gtataattat tgatatcgta agcgatagct
    48361 ggaacttttt cagcagctgc ttcgatttca gcaaccgaac gttttgtcac acgtgcagca
    48421 ctttctttag acatcgcttg gacggtaatt tctttattgt ttaaagcaat caagcggctt
    48481 gattgtaatc catgtgctaa aaatgcacct gaaatagcat ttcctgcaat caatccaata
    48541 actggatgtc cagcttgacg gagttttgca taagcagcag cactattagc caaagccaca
    48601 tggataccga tcaattcttc gttatagccg tatgcttggc ttggtacatc gatcacaacg
    48661 atgattggcc gtttcacttt tttgtcttta tcttcttcgt aaacgtgatc caaaatttgt
    48721 cccatgcgga aaccttcaac taaaccaact tcaccgtttc gtacacggta catcgggttt
    48781 ttaggatcag gtacgattga tgtcacgatg gcttcttcta tgaatttact tgatttacca
    48841 taatacacag tactaataga agaagttgga tttttcatgc cagttagtgc cgcaaaccaa
    48901 tcgtaaccaa cacttgctgc agctggttca tttccttctg ctacttctgg taagtcaatt
    48961 gattttgctt gatggttagc aattgcttca ttgtaatcgt caatggccat tggttttgaa
    49021 agatctaaag aatcaagtaa tgataaataa aatgctgagc tttcactgcg atgcgtgtct
    49081 tttcgttcgt ccattgctgc gatgacagca gctttgattg cttctacact atcagtcact
    49141 aattcatcaa tgatttttga ttctacccgt tgacgagatc ctaatgtatt ccaaatcaag
    49201 tctttgtcac tagaatcaaa ttctcgaacg cctgcttctt gttcaataac ttctggtcca
    49261 ttcaaaccga ctctcgcttt ttttgttgcg attaaataac tcattaatgc tgaagtaatc
    49321 gacataccgc caaatgatcc tacacgtcct ggaatcaaac cgatgactgg tacatattgt
    49381 tttagagcaa tgattacatt tccgatttct gaaatagata acagtccgta gtttgcttct
    49441 tgcaaacgaa caccacctgt atctagtaca atgactggaa agatctcatt tcctttttta
    49501 ttttcttcta acacatgttc aagtgcagca acgatttttg caccggaaac ttcaccgata
    49561 ccgccacctt ggaattttcc ttccatagcg attactactg cttgtttgcc attgatcgtg
    49621 ccacggctga caacgatccc gtcatcactt tcagggacga ttccttgtgc tggtaaactt
    49681 ggcgcgatga tttgatcgaa tggatcaatc aattcacgtc cattgtcaag tagagcgttc
    49741 actcgttctc tggcgtttaa ttctacaaaa ctatttttca tctgaaagtt cctccattgc
    49801 ttgcgtcaat cgtaggtaca caactcctgg tgtagcaccg aagtcattga tcacgatatc
    49861 tgctgaaatt ggataacgat caaaaaatct cgttaataca ttgttccata cttcgccgaa
    49921 gccatcgctt cctgtacaaa tttttacagt agattctgtt ttttctgttg gaaacatcaa
    49981 gatttccaaa tctcctgaac cgaccactcc tacatggatt ttcttattga tcggctgttt
    50041 cgttggatat ttataagcta attgttccat ggatctctcc cctttttgta aaacagagca
    50101 tacacgttct gcttattcgt ctttcttctt ccatctgggt aaatggcgac aaaaggtttt
    50161 tctcgccaat tttatgacca agttctgaat ttcattggtg gttcatacag gccatctgac
    50221 cattcgatca aatcatcgat ggtttgtgca gctaataatg aacgtttcgc ttcactacgt
    50281 ttgatgccta gatcttctgg taatttcacg atgccggctg cacgcaagct atctagtgat
    50341 tctttcgtgc tagtcattcc aagaggtgta acaccgccaa ttgctgcaat tgctgcttgt
    50401 cgttcttctt ttgtttttgc tttgtacaaa taagcaattc cttcttctgt cacgatatgt
    50461 gtagtatctt cgctataaat catcactggt gcattggcta agcctgcttg tttttgaaca
    50521 gctagtgcat ctaattcttc gacaaagact ggttttttat ttgaaccata tgtttcaacc
    50581 atttgaacaa ctaatttttg gcctttccct aatggatcat cattgtcgcg aagtgattgc
    50641 catgctggtg tagaatgacg acgaccgcca ggattatgtc ccatgttagg cgcaccgcca
    50701 aaaccagata aacgtccttt tgttacggta gaagagtttc cttcgtaatc taattgcaag
    50761 gttgaaccga taaacatatc gatggcatat tgtcctgcag cttgtgataa tgttcggtta
    50821 ctgcgcatcg tgccgtcttt accgacaaaa aagacatctg ggcgagcagc aatatatttt
    50881 tccataccaa cttcgccacc aaaactatgg atgctttcaa cccaacctga ttcgatggct
    50941 ggaatcatcg ttggatgtgg gttcaatacc cagttcttcg cgattttccc ttttaaccct
    51001 agtgattcac cataagttgg cagtaacaat tcgatagctg ctgtattgaa tccaataccg
    51061 tgattcaaag attgtacgcc gtgttctgcg tagattcctt tgattgccat cattgccatt
    51121 agaatttgca attctgtgat gttttgagga tctcgtgtga ataaagcttc taattgataa
    51181 ggtttatctg ctttcacgac aacgtctacc cagtcaccag ggatatctac acgtggtaaa
    51241 gaatcgacaa cttcgttaac ttgaacgatc acgataccat ctttgaaagc tgcagcttcg
    51301 atgattgttg gtgtttcttc cgtattgaaa cctgtgtaca agttgccgtc tttatctgct
    51361 ttatctgcag ctactaaaac aacatttgga atcaaatcga tgaataagcg tccataaagt
    51421 tccaaataag tatggatatc acctaatttc attgtgccgt cttcaatcat ttgggcgaca
    51481 cgcaagcttt gtgcaccagc ataagagaaa tcgatcttgc ttgcgattcc tttttcaaaa
    51541 atattcaaat gttctggtcg tgagatacta gacatgatca aatgcagatc attcactttt
    51601 tctgcatcta ctttttcaag actttctgaa agaaaacttg cttgtttttg attgtcgcct
    51661 tctaatacaa ctttgtcgcc gggagcaatc agtttttcca ttacttctac gattttttca
    51721 gtttctacat acttgccatt gagcaagttg ctgatttttt ccaatcgctg cgcttttttt
    51781 tcgcgtttag aagcccagtt gcggtttggt ttttccataa ttagtcatct ccatttttga
    51841 tatagaaacg tggcgtcatc aataccatgt tggtctagat tgatcgtacg gatcccttta
    51901 tctacaccaa attttgtatg aatcagcttt gtcaaagtat tgccgggggg aaattcaatc
    51961 gtcagatcca ttcctaattc ttttgccaag tccatcattt ggttccactg cacagggaaa
    52021 gctaggtttt ccaacaaatc tttacgaatc tcttctgcgt cctgagtggc tcttccatcc
    52081 gtatttttta tatacaaaca tctagcatca gatagatgga cagactgcat atacggcttg
    52141 aatttcttga ccgtatcatt catcaagaga cagtgagatg gcacagggac tttcagcagc
    52201 ttcgctgttt gcgcttggtg ccgtttggct tctgccaacg taacggataa accttcgata
    52261 ctcccagata acgtatgttg catcggacaa ttttcatttg acagataaac tggatggtca
    52321 ggatcaaatg tttttgctac aatttcctca gcttcgcttc tcgtcacacc tactactacg
    52381 cccattgcat aaccgcttgg ataagcttct ttcattaaga ctgcgcggtg atagactagt
    52441 tccgctgcat tctcaaatct caaagatcga caagcaacag ctgcacaaaa agcaccaatc
    52501 gaatgtcctg ctgcaatatc aggctgaata ccgttttttt ccatttcatc taaataaaat
    52561 actgcttttg tcagtaatgc tagctggata aaaaccgtat cttgatagtt ttcttcttta
    52621 tcaactaacc gataccccgt cacattttct aaacgttcca tgtacatttc agggatatcc
    52681 gctaacatcc ctaacttctg actgccttgt ccaggaaata agaaacctgt actcatgagc
    52741 ctcgcctcct cagcacttac tttttccact gctatcttag tcaatttctg ctataataaa
    52801 aaatgccaaa aaaagaaaaa accataacaa aaaggtatgg atgggatgga taattatgaa
    52861 attgcttcat ctgcagtatt ttaaaaaagt attcgaaaca aaaaatgtta cccaagcagc
    52921 caaagaatta tttatttctc aacctgcctt gagccgtgcg atcaaacatt tggaaaacga
    52981 attaggtgtc cctttgtttt accatagcgg cagaaacatt gagacgaccg tttatgcaga
    53041 agaattttat ccatatgcag tcaagacatt agacacatta gaagaaggaa tcaaattatt
    53101 gtcttcggtc aatgagaaga tcgttacttc cgtcatattg catttagagg tagcctctgt
    53161 ttctattccg aacttggtcc gaatttttat ggaaaagcat cctgatatcc aattaacgat
    53221 catgcaacat catttaccag atgatattac cgatcctgta ctgtacatca cttcggagat
    53281 gaagcctggc atgaccaata ttcctatcat gaaagaacct gtttatgtcg ccattcctaa
    53341 gaaacatcct cttgctaaaa aagaacatct gcaattagaa gatatccaac gcacccccat
    53401 cttgatgctg tcaaagagca acgccttccg tcatacactg gatgcagcac ttgaagcgaa
    53461 aagtatcgaa ttgacgatca gttcgacgac agatgatcct gcgacattac gttctattgt
    53521 caagcaagga ttagctgtta gctttttccc aaaagtttct tggtcttacg ataaaaatga
    53581 tcctttcgtc ttgcgggaaa tcaaagattt gcccttgaca cgaacaattt atttatcttc
    53641 tagcttcaca gaagatcatc ctttgatcaa aatgattgca aaaacgatgc gggaatttta
    53701 tttaggaaaa taaaaaaaaa agagctggga caaaattctt gtcgcagctc ttttttcgaa
    53761 caaacggtgt tccagaagta atttcttaga aaataagccg aaatttccca aaaatttgag
    53821 aagcaatttt cgtaaattcc ttcttatttc ttgaagctaa acatttctgt cacaacctct
    53881 tctctattgt cctagacatt cattgcgttg atttaaaact tccatcaatc aaatctgcta
    53941 gttcatctag cttttctatt ttacgtagat caatagagat ccaatgttct ttattcatgt
    54001 gataggctgg aaaatacccc tcttttcctc gcaaaattcc gccaagttca ggatcgacct
    54061 tcaaatcaag aatattgatg atcctatggt cacttgcatc atcatagatt ttttcttctg
    54121 gaacagacat caggacccca aaccattttt tgcttttttg atgtctgaat acgcaataat
    54181 caggaaattt tttaaaaata tattctggag acaaattgta tgtttcattg atgtgatcga
    54241 ttagttcttt tttcgttagc atctgcttcc atctctttct tattgattcg ctttattcaa
    54301 agataccacg taagaataga aaaatgaata caagtagttg ctaatcaaat acttcgatat
    54361 ttttcttttt tatcaactct taaatgttca atccgcctgt gttggtcttg ctaccatcta
    54421 ctattaaaat gtttattatt gtttgcccac ctattttttg attttagtgg tatcattttt
    54481 caagttcatg atgaaattct tatcatcagt aaatgctcca gaaaaataaa caatgattga
    54541 aaaatcagtt gaaacttgac caccgtttat tttctccgta ttcttagaat agaagggagt
    54601 ctgattgatg tattcactaa tgaaaaaaat cattaccgaa aaagatattc gacgtcatgt
    54661 ttctttagta gaacaattgc tgaatcatac aaagataacc gtaaatgaat tagctgaaat
    54721 catagggaca accgaacgaa cgattttttc tgaccttcaa tctattcgct ctcagctgcc
    54781 cgaaggctgg gacatcatca gcgatcaagc aggaatcagt ttgcaaaacc agcaaaattt
    54841 attaacaaat gacctttggg agattttttt caaacaatca gtcagtgtcg aactgctgaa
    54901 aaatctgctt tttacaaaaa aagttgctgt ccctgatttt ctagccgacc atgggctttc
    54961 ttatgggaca ttgaaaagac atgtgactaa aatcaatcag cgtttagcaa gttacgactt
    55021 gcagatcgat ttgacgaaat atacagcttg tattcttgga aaagagcgtg cgattcgtac
    55081 tttttatcat cgattgctga ttcctttcac tcataacaac tattttttcg aggattattc
    55141 gatccatgaa tcccattatt ttcaattttt aagaaacctc agtcaaactg aactagcggt
    55201 tgaaacagaa gagattttcg gaacatgttg gttctttatc aatacgatcc gtataaaagc
    55261 gaattgccga ttagattctt ctatccatac caacagcaca ctctcctccc tgtatgactc
    55321 tgcattgaaa aaattgtatt taacggaagg tatttatctt aaagacactg agctttcttt
    55381 tgcttctttc tgctttctgg aaagttggaa ttacaacaat aactatggac aagaaaccgc
    55441 tcgctgcctg catcattctc catttttaga ggtcctcgaa acgtttgtag aggaactagc
    55501 ttcagagctg tctcttgatc aactaaaaaa aacatctctg actgataact tggctctcct
    55561 catattaaaa tatcatgaat cgccgatatt gattgaagca ttggaccgtc aataccatcg
    55621 ttttttagaa acatatgatc agcaatatgc acacttgtat caacagaaag agaatttact
    55681 gaacgatcta aaaaaagaaa tagaaatcaa tgaaccaaat tactttctcc aactcttgtc
    55741 tctactgatc caacaaacga ttttttctat caaaccgaat ttgttcaatg tctattttat
    55801 tttccaagga gaaccttcat ggaaggcgtt cttgcagcag gaattaaaag attacttagg
    55861 aaaacgtgtc tgcttcttac ctatcgaact aaccgatctt tccactattt cttttgaaaa
    55921 aacagatatc ttgatttcca atttccctct agattctcta gatatcccta ttgtatacat
    55981 ttcttctatt ccaacgaaaa atgagctcaa tcgtttgact gaactcactt tcaaaacttt
    56041 tttatagtaa cggctagcga ctgtattctc tagctgtttt ttcttttttg cgaaacagtt
    56101 tgttcatgat ccctttttct atttctacaa ctaagaatag aagaatcaca tttgctccaa
    56161 tcaataactg ttgtgtcatc gttaaactta ctgtaccgat cagctgctgg ccgactggta
    56221 gaaaaataac catcccttgc aataaagcaa ggatacctaa tgaaagaaaa agcgctttgt
    56281 tttgcaaaag accttggttc aatgacggct tggacaactc tctgcaattg atcatgtaaa
    56341 cagcttgagc taagacgata ttttgcagaa gaatggtttg ctgtaagctt tgcccttcaa
    56401 actgcatagc caataaatag gcaggaacca tgatcagtag ggagacatag atgatccgaa
    56461 acaaatgata accagatagg attccttgct ttggatctct tggtgggcgc ttcatgattt
    56521 ctggatctgg cgactcgaac cctagtgcat aagataaagt gattgtcgtt accatattga
    56581 cccataaaat ctgtactggg gacaaaggta gcgggcgatt cagcataagt gcccaaatca
    56641 agatcaatcc ttgagctaat gcagttggca agaagaaatt gatggttttt tgcaaattat
    56701 ccaaaatccg ccggccctct tttacggctt ttgcaatcgt atggaaatta tcatctccta
    56761 aaaccatatc agctgcctgt ttgcttactt cactaccttt gattcccata gcaattccta
    56821 catcggatct tttcaaagca ggtgcatcat ttacgccgtc tccggtcatt ccgacgattt
    56881 ctccattttt ctgcaaagct ttaacaattc gcagtttatg ttctggtgtc gttcgagcaa
    56941 agacatcgac tattggcact tgctgaatca actcttcatc agacataaga tccaaatcga
    57001 tcccttctaa aactttggca gtatgtttta agccaatttg ttccgcaatc gcttttgctg
    57061 tgtctttgtg atcccctgta atcattttca ctgagatccc tgcttgttgg cattccttta
    57121 cagctttgac tgcactttct tttggcggat cgatcaatcc ggcgatccct gcaaaagtta
    57181 attgggttaa agtctcatgc gtgagttcct gtttcgaatc tactttttta taagcaaatc
    57241 ctaaaacacg ctgacctttt ctcgcaaatt ctgcagctgt ctgactccat gcttcttgtt
    57301 ctgcagaaga aagagttgat ttagcaaata atacttctgg tgcacctttg acgtaaatca
    57361 ccgctccttt ttcctccgca tgcatcgttg ccatatattt gtattcagaa ctgaacggaa
    57421 tctttgcttc tatttttttc aaaggtagat tggctgtttc tgtgtgtcga atcagcgcaa
    57481 cttctgtcgg attgccactt aaattttctg ttttctgttc ttcttttgtt tttaattcct
    57541 gacagttttt cataatggac aacacgcagg cttcttcttt tgcagctaca tccacaactg
    57601 tcatttcatt ttttgtcaat gttcctgtct tatctgaaca gatcactgtc atagaaccta
    57661 atgtttctac cgaaggaagt cctttgatga tcgccttttc ttttgccatt tcatgtactc
    57721 caacggatag aatcatggtc aaaacagctg gcaatccttc gggtaccatt gacaccacca
    57781 aagcaatcgt agccgaaaga agaatatgcc aatccatacc atatcgaaat gtcgtaaaaa
    57841 taactaagaa taggctcaaa caaagaatgc cttgaaaaat ttgtttgttt aatcggttga
    57901 tttttttaat cagaggcgtc gtttgttgtt cgaccgattg caatgcttga ttgattttac
    57961 cgatttctgt cgtgtttcct gtttctacaa cgacaccgac tgcagaacca gattgtacaa
    58021 gcgttcctga aaaacccata tttttctggt cgccgatcga ttcttcttct gtcaaaacgg
    58081 catgactttt ttcagatgct tctgattctc ccgtcaaaat cgattcttcc actaataaat
    58141 catgtgcttc gattaatcgg atatctgctg gaacgacatc tccggcacgt aagacaatga
    58201 catctccttt taccagttct ttcgcaggga ttttttcttg catcccgttt gatagtacta
    58261 tcgcttcttg tcccatcagc tgcttcaaac cattcaatga ttcttccgct tttctttctt
    58321 gccagtaact aacgagtcca ttgacgatca ctacgaataa aataatactt ccttcaatat
    58381 attcactaga agcaaatttc aaaaaagagg caatgatcaa cacgatcatc agcagatcag
    58441 taaaatgctt tgctattttt ttccatcgtt ttgtctgctg ccttgctttt atttcattat
    58501 atccttgatc agtcaggcgt ttctgtcgtt cttcttgcga caagccttct gttgatgttt
    58561 gatagttttt caatacatct tctaccgtca atctgtacca gttcatccta tcccctcctg
    58621 tttaattgat actgttatta tacgcaggga agttttttca ggcgtatcga ttatttcagg
    58681 ggggtctgaa ataattcgtt ttgcaaaatg tatctgggcc taactgatat ttctattgct
    58741 tattctttta tatacaaaat ttaagaatac aaaccagata accaataaaa aaggaataaa
    58801 gcgtgtcgtg ctttcgaata acaagctgaa agcaattgaa gtaaaaaaca ccaacaaagc
    58861 aagatcttta tatctttgtg tagtgttccg tttcttttct ttaataaatt gaacatgtgc
    58921 acacaagatc gaagaccaca caaatagaaa acaactcgtt gtcatgcctg ccaaaagctg
    58981 aaaaaacaat cgactatcag ctacaaaaaa atgtagacaa agaccggcaa aaaataacat
    59041 tcctgtaatt aaaataccgt gtaaaggtac gcctttctta gaaagcgcac aaaaccaatg
    59101 gggcaaactt ttttcttttg ctgtttgata taaaatcctg ctagtactgt agactgcact
    59161 atttgctgag gacaaagaag caaccaaaat cattagattg acaattgctg ctccgccttg
    59221 gcaatggatc acttgcagca tagtcactaa aggactttct tttaaatcta aacaatgcca
    59281 tggaacaaca aataaaatcg acaaaatcgt tcctatatag aaaaagagaa tacgccaagg
    59341 cagacagttg attgcttttt tcaaattctt ttgcggatca cttgtctcgc caacggttaa
    59401 gccgatcatt tcaattccag caaaactgaa taaaaccata ggaaatgaga ctaccattcc
    59461 agcaaagcca aacggtagta attcatcgat ctgtccaaaa ttttctttga ttccgataga
    59521 ctgggtatga aaatctgaga cactcaatcc tatacccaat aagataaaaa ggaaaatccc
    59581 caagatcttg atgattgaga aaaaagattc caattcacca aaccacttga ctgcaacaag
    59641 gttgactgcc attaagatca ttagtgcgct acctccgctt atcaaagctg gtacatgtac
    59701 aaaccagaaa tggatataca atcctattgc tgttaattcc gcaagtccag ctccaagcca
    59761 gcaaagccaa taagtccaat tgacaaaaaa tgcccacttt ttgcctaaat aatagtcaac
    59821 gaaatcactg atagagttaa atgtactatt tgctaaaaat aaatcaccca tggcccgcat
    59881 caaaccataa atagccaaac cgcatatcaa ataaacgaga ataacagctg gccctgctga
    59941 tgcaattgcc tttgctgaac ctaaaaataa tcctgttcca attgcgccac ctaatgcgat
    60001 taactggata tttcgattag ttagagtccg tttcattgcc ggtttttcca tcaagttcac
    60061 ctttttcttt ttcaagcagc atggcgactt cctgttccca agaaggtttc tttatttttt
    60121 caatacagag tggtactaca aacaaaatga cactgatggc tgtgactgca attagattga
    60181 tccatgttcc agctttcatc gtcagtccga cagaaaatcc gattgccgca aataaaacaa
    60241 taacgacagt ccaaactcca agattgctct ttttctcttt ttttccaatt ctaaatggtc
    60301 gattcaactt tgcatgtgta taacgcaatc tcaaaactgc gatccccatg atgacataga
    60361 cgatacagta gataacagta gttgaattta cgagcatcaa aaatgcttga ttgacgccag
    60421 gaatcagcaa ataaaaaagt gcaaagattg agataatgac agactgagtc aaaataacgg
    60481 atttggatac gtcaaattga ttcgtcttcc aaaaattgaa ttttggtgga tacgcacctt
    60541 ttctagcact ttctgtcact gtctttgctg gtcctgaggc ccaagcagat aattggactg
    60601 ctacaccaat gaagacaagt aaactaaaca gattcacaat aagatgtggc aaaccaagaa
    60661 tttggcaata aatcgaaata gattgcgcga tattattcaa ttccatttgt ccttttggta
    60721 cgacatttgc tacaactaaa gcattcaaaa tattgacgaa aaatataaaa attaacgcgg
    60781 caaatactcc gcgaatatac gttttaactg gattttccaa tcgagtgata tacacagagg
    60841 acatttcgat accagtaaaa ataaacatga tcggcgcaaa ataaacaaat gaggatgcag
    60901 tcgtagtatc tggtattaat ttgtcccatg aaaaactgcc taatgttccg gttggtacga
    60961 tcccaacttt gatccacgca gcaaacccta ataagagcat catcgtcaaa ggcacgtaca
    61021 atcctaacca aacaccgact tttccgccga tttttgccat atcaaatttc aaattcaaaa
    61081 aagtgatgat ccaataaacg actaaaatga caactaatgt aaatttgctg ttcaaaccaa
    61141 gcggcacatt atcgataata ttaccaaaca acggagcgag tgctgaagcg accatcacca
    61201 tcccaggaaa catttggacc cataacagcc atgagacaac gaacccccat ttttttccta
    61261 gagcattcga tacccaaagt tctggaccac ctttttgggg aaacattcca gcaaattcac
    61321 cagagatcaa tgagattgga aaagcgtaca ataatactgc aaccagcatg taaaaaatca
    61381 tcgtccagct cgttgctgct acatctggaa tatttcgtac acttgctaca agggcgcagg
    61441 tcattccgat aaaggtcatg gtcgtgattt gctttgatga accagtatct ttcatcttta
    61501 ttccttcttt caatagcgac atatgttttt gttttctttt atttttgttc aagaacagaa
    61561 atgacaaggt ttcatcgatt cagtacaaaa cccagtatca ccgaaacatc cattgaacga
    61621 tcttagtgaa ctgggtttga tacatgaaac gaacgatcca acttatagaa cgttttataa
    61681 aaaagaatat cttggcgagg aatatatgaa aaacaggggg aaatacaatt gttttgatga
    61741 agcaaataat tgtctgttct ccatatgtct cttgttattc tttcacacaa aaatcagttc
    61801 tattggatga tttcgtttag tttcttttcc attgctttgg cgattttttt tgtataaaaa
    61861 ttgaaaattc gatcatcatt caaataagga gtcatgatag ccgcacgtag cagagtgatt
    61921 ttctgttctt ttttccattc ttctttcgtt agacccattc tttcaacaaa acgaatcgga
    61981 gaatcaccgt aatcttcttg tgtaaatgta gtatgagaag taataaaccg ttcaccataa
    62041 acatcgccat ccatataaga agaccgatcg aacatttttt catttaattc attgatagcg
    62101 ttcaaatctg tacagccttg ttctttgaat acccaattga ccatattaaa atcaggatga
    62161 ttcaatggat agacttcgat tgtttttcct ttaacagtaa aagtcagatg atcgagaaat
    62221 tctctgaatc tttgagctgc ctcgattgaa gccccaatca attgaccata tccggtcaca
    62281 ttcaatggca atacacgatg tgctgtccag actgcggcgg cggttgctcc agcttttgat
    62341 ccttccaaaa tataagctcc taacatatct ggagccttta ctgatttttc aaacacatat
    62401 ggcgcgaaat aagaaatgat atttctcata tttttatgtt tgattacgat tcctccagca
    62461 gcataaggga catagcccat cttatgagga tctatcgtga cagaatccgc ttctgaaata
    62521 gctctgaacc cttcatatac ctctttgtca atcgttacgc agtggtggaa aacatgatgt
    62581 tcttcaaaga attcagctaa ggaagcatac ggcacaaatt ctcctgcttc gtttagaaac
    62641 agtgaacgcg catagccgcc atatgcggca tcaacatgta aatagaaata gatcccttca
    62701 tcttttaacc tttctcttaa ttggacgatt ttatctacac tgtctacttg accttcttct
    62761 gtcgttccta cgacagcaac cacacctaga attgggattt tttgtccagc taattcacga
    62821 attgtttttt ccaaagcatt aatatccatt cgataatcct cttgaactgg gatagcaacc
    62881 atctgatcta agccgactcc gcaaatatct aacgccttca tccaagaata gtgttttgtt
    62941 tgagggacca gccatttccc taatctttgg atattttttc cgctacggga agaagctgct
    63001 ttgacctcat ccatttcttc gtccgtaaag ctttcggtca tttccaaaat ttcttcaaca
    63061 gataaattca aaagttccca ctcagacatt tttttcactt tttcaggcag cacttccttg
    63121 acagctaaag gaatcgattt gatgcaacgg gcgtaccata atccttcaag attagcaata
    63181 gaaccatctg ctgcaatgtg tccccatccg tctgtcatat tgaataatga agcaaaatcc
    63241 tgtcctactt ctgcttccat ttgcgaagtt gccatagaag attccaatgc aacattattt
    63301 gggttccaca gcatcgcata gttgtaagct aataaagcag gcatcaatgt ttcagcattc
    63361 atttgtcccc agtaacggcc tgctgaatgc catgggatcg atcctgcgcg taatcgctga
    63421 ctgacttcat ccaagacctc tatcatatgg tcgcgggtgg ctagataatc tggagtgagc
    63481 ttatctcctt cgctaattgc aggcatatct gaaggaatat aattttctct ccatccaaga
    63541 tgttcatcta ccatcttgtt caacaacatt ttataaactg gtccattttc agctttgtcg
    63601 cctataaaga cggccttgat atccatatct ttcatttttc ttccctccat attttgttgg
    63661 tcaatgtctt gcagatacat gtgtttatct gtaacacctc ttcactatag cgccaaaaat
    63721 gagaattttt tttttataac atcaaatcca cgtagatttt tacaaacaaa aataaataaa
    63781 gtcggatttt tctgataatt caacgcaaaa aagcgaacaa tttttcaata aatcagtctt
    63841 ctaaaataga taacctaaaa aaagcg
//
LOCUS       DAZUKT010000004        62200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000004 DAZUKT010000000
VERSION     DAZUKT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.004"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            538..1374
                     /locus_tag="ACG816_000255"
     CDS             538..1374
                     /locus_tag="ACG816_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4919175.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            1438..1869
                     /locus_tag="ACG816_000256"
     CDS             1438..1869
                     /locus_tag="ACG816_000256"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4919176.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(1931..3253)
                     /locus_tag="ACG816_000257"
     CDS             complement(1931..3253)
                     /locus_tag="ACG816_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4919177.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3407..5047)
                     /locus_tag="ACG816_000258"
     CDS             complement(3407..5047)
                     /locus_tag="ACG816_000258"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS4919178.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5380..5850
                     /locus_tag="ACG816_000259"
     CDS             5380..5850
                     /locus_tag="ACG816_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4919179.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            6072..7175
                     /locus_tag="ACG816_000260"
     CDS             6072..7175
                     /locus_tag="ACG816_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4919180.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            7459..8139
                     /locus_tag="ACG816_000261"
     CDS             7459..8139
                     /locus_tag="ACG816_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4919181.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            8229..9263
                     /locus_tag="ACG816_000262"
     CDS             8229..9263
                     /locus_tag="ACG816_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4919182.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            9277..9612
                     /locus_tag="ACG816_000263"
     CDS             9277..9612
                     /locus_tag="ACG816_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919183.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            9596..12076
                     /locus_tag="ACG816_000264"
     CDS             9596..12076
                     /locus_tag="ACG816_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919184.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            12132..13691
                     /locus_tag="ACG816_000265"
     CDS             12132..13691
                     /locus_tag="ACG816_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919185.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            13688..15052
                     /locus_tag="ACG816_000266"
     CDS             13688..15052
                     /locus_tag="ACG816_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4919186.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            15223..16293
                     /locus_tag="ACG816_000267"
     CDS             15223..16293
                     /locus_tag="ACG816_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919187.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            16308..16976
                     /locus_tag="ACG816_000268"
     CDS             16308..16976
                     /locus_tag="ACG816_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919188.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            16996..17574
                     /locus_tag="ACG816_000269"
     CDS             16996..17574
                     /locus_tag="ACG816_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4919189.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            17975..18886
                     /locus_tag="ACG816_000270"
     CDS             17975..18886
                     /locus_tag="ACG816_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4919190.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(18974..19891)
                     /gene="rnhC"
                     /locus_tag="ACG816_000271"
     CDS             complement(18974..19891)
                     /gene="rnhC"
                     /locus_tag="ACG816_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4919191.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            20093..20530
                     /gene="zapA"
                     /locus_tag="ACG816_000272"
     CDS             20093..20530
                     /gene="zapA"
                     /locus_tag="ACG816_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4919192.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            20589..21137
                     /locus_tag="ACG816_000273"
     CDS             20589..21137
                     /locus_tag="ACG816_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS4919193.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            21252..23612
                     /locus_tag="ACG816_000274"
     CDS             21252..23612
                     /locus_tag="ACG816_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4919194.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVCGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            23699..24013
                     /gene="trxA"
                     /locus_tag="ACG816_000275"
     CDS             23699..24013
                     /gene="trxA"
                     /locus_tag="ACG816_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4919195.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            24195..25973
                     /gene="uvrC"
                     /locus_tag="ACG816_000276"
     CDS             24195..25973
                     /gene="uvrC"
                     /locus_tag="ACG816_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4919196.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            26118..27017
                     /locus_tag="ACG816_000277"
     CDS             26118..27017
                     /locus_tag="ACG816_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919197.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            27010..28272
                     /locus_tag="ACG816_000278"
     CDS             27010..28272
                     /locus_tag="ACG816_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919198.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            28433..30331
                     /locus_tag="ACG816_000279"
     CDS             28433..30331
                     /locus_tag="ACG816_000279"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS4919199.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(30661..31380)
                     /locus_tag="ACG816_000280"
     CDS             complement(30661..31380)
                     /locus_tag="ACG816_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4919200.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    31570..31617
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            31717..32049
                     /locus_tag="ACG816_000281"
     CDS             31717..32049
                     /locus_tag="ACG816_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919201.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            32036..34039
                     /locus_tag="ACG816_000282"
     CDS             32036..34039
                     /locus_tag="ACG816_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4919202.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            34058..34528
                     /locus_tag="ACG816_000283"
     CDS             34058..34528
                     /locus_tag="ACG816_000283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4919203.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            34549..35133
                     /locus_tag="ACG816_000284"
     CDS             34549..35133
                     /locus_tag="ACG816_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS4919204.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            35148..36134
                     /locus_tag="ACG816_000285"
     CDS             35148..36134
                     /locus_tag="ACG816_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4919205.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            36124..36435
                     /locus_tag="ACG816_000286"
     CDS             36124..36435
                     /locus_tag="ACG816_000286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4919206.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            36457..38238
                     /locus_tag="ACG816_000287"
     CDS             36457..38238
                     /locus_tag="ACG816_000287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4919207.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            38231..39607
                     /locus_tag="ACG816_000288"
     CDS             38231..39607
                     /locus_tag="ACG816_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4919208.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            39614..40249
                     /locus_tag="ACG816_000289"
     CDS             39614..40249
                     /locus_tag="ACG816_000289"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4919209.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            40279..41634
                     /locus_tag="ACG816_000290"
     CDS             40279..41634
                     /locus_tag="ACG816_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4919210.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(41826..43220)
                     /locus_tag="ACG816_000291"
     CDS             complement(41826..43220)
                     /locus_tag="ACG816_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4919211.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            43312..44283
                     /locus_tag="ACG816_000292"
     CDS             43312..44283
                     /locus_tag="ACG816_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4919212.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            44280..45062
                     /locus_tag="ACG816_000293"
     CDS             44280..45062
                     /locus_tag="ACG816_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4919213.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(45231..45602)
                     /locus_tag="ACG816_000294"
     CDS             complement(45231..45602)
                     /locus_tag="ACG816_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4919214.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            45736..46179
                     /locus_tag="ACG816_000295"
     CDS             45736..46179
                     /locus_tag="ACG816_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4919215.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(46222..47031)
                     /gene="yidA"
                     /locus_tag="ACG816_000296"
     CDS             complement(46222..47031)
                     /gene="yidA"
                     /locus_tag="ACG816_000296"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4919216.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(47050..48417)
                     /locus_tag="ACG816_000297"
     CDS             complement(47050..48417)
                     /locus_tag="ACG816_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4919217.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(48401..49231)
                     /locus_tag="ACG816_000298"
     CDS             complement(48401..49231)
                     /locus_tag="ACG816_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4919218.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            49394..49831
                     /locus_tag="ACG816_000299"
     CDS             49394..49831
                     /locus_tag="ACG816_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4919219.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            49907..50527
                     /gene="rpoE"
                     /locus_tag="ACG816_000300"
     CDS             49907..50527
                     /gene="rpoE"
                     /locus_tag="ACG816_000300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4919220.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            50716..51222
                     /locus_tag="ACG816_000301"
     CDS             50716..51222
                     /locus_tag="ACG816_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4919221.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            51239..52138
                     /locus_tag="ACG816_000302"
     CDS             51239..52138
                     /locus_tag="ACG816_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4919222.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            52225..53109
                     /locus_tag="ACG816_000303"
     CDS             52225..53109
                     /locus_tag="ACG816_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4919223.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(53301..53372)
                     /locus_tag="ACG816_000304"
     tRNA            complement(53301..53372)
                     /locus_tag="ACG816_000304"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53337..53339),aa:Arg,seq:ccg)
     gene            53432..54766
                     /gene="rpoN"
                     /locus_tag="ACG816_000305"
     CDS             53432..54766
                     /gene="rpoN"
                     /locus_tag="ACG816_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4919224.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            54915..56006
                     /locus_tag="ACG816_000306"
     CDS             54915..56006
                     /locus_tag="ACG816_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4919225.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            56047..57048
                     /gene="gap"
                     /locus_tag="ACG816_000307"
     CDS             56047..57048
                     /gene="gap"
                     /locus_tag="ACG816_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4919226.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            57158..58348
                     /gene="pgk"
                     /locus_tag="ACG816_000308"
     CDS             57158..58348
                     /gene="pgk"
                     /locus_tag="ACG816_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4919227.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            58452..59207
                     /gene="tpiA"
                     /locus_tag="ACG816_000309"
     CDS             58452..59207
                     /gene="tpiA"
                     /locus_tag="ACG816_000309"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4919228.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            59323..60621
                     /gene="eno"
                     /locus_tag="ACG816_000310"
     CDS             59323..60621
                     /gene="eno"
                     /locus_tag="ACG816_000310"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4919229.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            61020..62159
                     /locus_tag="ACG816_000311"
     CDS             61020..62159
                     /locus_tag="ACG816_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4919230.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        20538 a        10900 c        13102 g        17660 t
ORIGIN      
        1 aagggattgt gacaaacgtc ttgtcacaat ccctttttcc aaataaacga tgttcaatca
       61 gctgcccctc ggcattcgct cgaaaaacgg cgaaatatga agaactattt tgccatttgt
      121 ttctctaata atcaggtcaa aacgttgact tcacagtctc ttccttaaac ggtgttctcc
      181 tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa
      241 ttgttccttg tgactcggag ctgaacggca ggctcacgac ctctttctta aacggtgttc
      301 aaaagcttgc atctgcggta ataagttcaa ccaaacgaaa attatgagga gctatttttg
      361 tttggctgtt cttattactt gatgcaggac agctttttca caacctcttt tcgtttagca
      421 ctaaatttcc ttaaaaagta gaaaagcttt cgaagcctcg tttgtttctt ttactgtata
      481 ataaaagaaa agagtaacaa tcgctaaaag atataaacaa gagaaaggaa atacaagatg
      541 attggtctga tttattttgt ggtgatcgtt ctggcgaaca cagttggcgc tatttccggg
      601 atgggtggag gcgtgttgat caaaccgatt cttgatctta tcggcgccca ttctgttgca
      661 gggatttctt tttactcaac agttgcggta ttcacgatgt ccattgtttc gactgtgcga
      721 caagtatcaa gcgggaaatc actgaattgg cagattgtcg gttgggtatc tagtggagct
      781 gttgttggag gagtagtcgg caacattgta ttcgaagttt ttttacagtt atttgaaaac
      841 gagaagcatg tgcagctgat ccaaattttt ttgacggtat tgaccttggt ttttgcgttc
      901 ttctacacca agcatcatca gccaaaattt catttgacgt catggacttg gtaccttata
      961 tgtggggggg ttctgggctt tttagctagt tttttaggta ttggtggtgg accagtcaat
     1021 gtttctttgt tgatgctgat gtttgcattg ccaataaaag aagcaacact atattctcta
     1081 tctacgattt tcttttctca actggcaaaa ttggtaacga ttgctttaac aagtagcttt
     1141 atgcgctttg atttatctat gttgttttat gttatccccg ctgctgttat tggaggtttg
     1201 tggggagcaa gatttagcag ggttttgtct ccaaaaaaag tgacctttat ttttcaagta
     1261 attgtcctcg tggttctttt ggtcaattgt tacaatgcct ttgtcatctt acaaacatta
     1321 ggagaatcta gactaacgca agtcaaaatc actgaaacat attgttctaa atgaaaaaag
     1381 gagtatactg tgataaaaaa attcatttaa aaaaataaaa attggctggt gatttggatg
     1441 ttgcgggaaa tgcgtttagg agatgcagaa gcactacgag aaatcaatgc ggaacaatta
     1501 ggatatgatg taccattaac cgttacggtg agacaaatga agaaactgtt gaaggagcca
     1561 gaaaaaaatt ttttcttggt ctatgaagat cctacttcta aaaaagcagc tggctatgta
     1621 caagcggagt cctatgaatc gattttcagc gatccgatgt tcaatattat ggcacttgct
     1681 gtgtctaaac atgctgaaaa aagaggaatc ggaaaggcct tgatgactgg cgtagaagaa
     1741 gcgaaaagac gaggaatatc agcaataaga cttaattctg cagaatacag agtagaagca
     1801 caccgatttt atgaacgcat cggttatcat tccgataaaa tgcaaaaaag atttttgaaa
     1861 atcctgtaaa tttttatcaa aaaatacctg gtcatcaagc cgatacttga tgatcaggta
     1921 ttttttttga ctaagaaagt aattgcatca agaaacctga gatgatcaaa acgatggcac
     1981 caccaagccg attgcccatt tgagcaaaag caataagttc catccgatta gctgcagata
     2041 agacagctac atttcctgta ccacccatcg agttgttgca taagccagca gtaatagttg
     2101 attcgattgg atataaacca aacaaacgac cgacaaatcc tgaaactact gaaatcgcga
     2161 taacacttgt caaacataag acaacaaatt gccatgtcaa agcagaagcc aatacattta
     2221 gattcaataa cgcaataccg ataccggcaa gaacagctgc tgtcaagttt ttgacaatca
     2281 agttgttgaa catgatagct gcatcttcgt aatattctgg taataggttt gcgattttac
     2341 agataacaac gataatgatc atgaatgcgt aagcatggac tttaggaaca aaatagttac
     2401 agatttgtcc taagatgaag aaagtcaatg ataccatcaa accgacacct aattgtggaa
     2461 tggaaggttt tacatttcta ggttttccat cgccaaggtc gcctttttcc attaatttac
     2521 cattaccgtt gaattttggc attgattgtc ccgcacgagc aactaacgct gcaccaatga
     2581 tagctaggac atttgtcatt gctgtcgcag gaatcaggcg ggaaatgata gaagaagcgt
     2641 ccgtacctaa aacatttgca taaatcgttg ataaaggtac agctccggct ccaacaccac
     2701 cagccattgc gggcaatgag atatataaaa cggaatttgc aaaaccattt ccgataagca
     2761 tacctacgag tcctacggca aaaaaggaga cgaccattga aataagtgca acgggaataa
     2821 aacgaacaga agctttcatc aatagattac ggttcatacc aaggatgcta ccagtaatca
     2881 aggcagcaat ataaaagtct aaaaagccca tattgctgac aaagttttct gtggatttta
     2941 ctacatactc aggtaagatc ccaaatgtag caatcgcggc agaggcaaag atcgcaaaaa
     3001 cggagccacc acctaaataa cttttgacga tagggatttt tgttccgata aaatgaaata
     3061 aattaccgaa aatgaccaaa acagcgattg ggccaatcat attcgtcggt aattttccta
     3121 aagccattgc aatgatcagg acaaggacca tgatcaaata aaggggcaaa cttacgccgg
     3181 taatttttgt tgaccaaaac ccttcttttt tcttttcctc aactgcagtt gcggttgttg
     3241 ttttttgtgc catttttaca gatcctttct tattctgtat cgtatgaaaa aagccaaaga
     3301 gaagcggcaa aagagcagta gttggctggc tgctgttttg ccgcagtcgc ttttttcttt
     3361 ttagacgtca tacaaagttt ctgtatgttc ttttactata acaatcttaa tcttctaaag
     3421 atttatattc aggaacccat ttagcagatt caacagcttc tttgatatcc gtgatttctt
     3481 cacgattcaa tttttgtgca acaactgatt ttgcgacagt ttcagcaatc gtttgagaga
     3541 attcagtgat ttttgctact ggaggcaaga tagctgctcc tggttttgtt acatcaacga
     3601 ttccgcctaa tgctcgacta gcttgagaaa tgatttcagc atttactcgt gtagctgttg
     3661 aagcaatcaa accaagccct aaacctgggt acatcaacgc attatttgct tgaccgattt
     3721 ggtaagttac accgttatat tccacatcag cagcagggat acctgttcca actaaagctt
     3781 ttccgtccgt ccattcaatc aaatcttttg cttttgcttc tgccaatttt gttggattag
     3841 acaaaggcat gatgattgga cgtggggtat gagccgccat ttctttcacg atttcttcag
     3901 tgaatgctcc aggttgggta gaagtaccga tcatgatcgt tggatggatt gcttttacaa
     3961 ctgcttctaa gtttgtcaat tcatcagcat tagtgaattc tgagcgttta cgagcgaatg
     4021 ggatttgacc aggagtcaat ccttttgtat cttcaaacaa taaaccctgt ttatctactt
     4081 gatagaaatg ttggcgtgct tcttcttcag gtacaccttg acggatcatt tcatctaaaa
     4141 ggatgtttgc gatacctaca ccagcagtac cagcaccaaa tgtcaagatc gtttgatctt
     4201 tcaattgctc acctgatata ttcaatccac ctaatacacc agctagtaca acgattcctg
     4261 tgccttggat atcatcattg aaagtagtga ttttatcttc gtatttttct aagatcgttg
     4321 cagcatttcc tcgtccaaaa tcttcccaat gtaataacaa ttctgggaat aattcagtag
     4381 ctgattgaac gaattgatca atgaattcgt agtaagtttc gccttctaca cgagcatgac
     4441 gattccctaa gtataatgga ttattcaata attcttcgtt attagtacca gcgtcgattg
     4501 atacaggcaa gacttgtgca ggattgatac cagccgcagc agtgtagacc atcaatttac
     4561 cgatagcgat gtcaacaccg ttcacacccc agtcacccat acctaagatt ccttctgcat
     4621 ctgtcacaac gatcaaacga atgtcgcgtc catctgctgc gtttttcaaa cttgctttga
     4681 tattttctgg ttcgtcgatt gaaagaaatg cagcatcttg tggtttcaag aaaatttcgt
     4741 tgtattgttc gattgaatcc gcaacgacag gatcataaac gacaggcatg aattcaacta
     4801 aatgttcgcc catcaattta taaaacaatg tccggttcgt attgaataga ttcattaaga
     4861 agatacgttt ttccaaatcg gtctgtttgc ttaagtattg accgtaagct tggacagatt
     4921 gttgttccaa tgtttggaca gtacttggaa gcataccagt gattccgtat tttgcccgtt
     4981 cttcttttgt aaaagctgtt cctttattta gaaaaggatt atttaataag tttagccctg
     5041 taagcatgaa tgattcctcc taagttttaa tacaaacgaa gtataggagt cagatgagga
     5101 tatagtcaaa aagagcaggt ataataaatt ttatttatat gagtaataga gtcaaaagaa
     5161 atcaaaatag cggtatttgt tctttttata tcaacaatcc ttgcttgatt actatgcgta
     5221 tctcttatat gctaaagaaa gaaaagtaga agaataaagg atatgttaga tagataaata
     5281 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
     5341 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
     5401 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
     5461 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
     5521 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
     5581 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
     5641 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
     5701 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
     5761 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
     5821 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
     5881 gacacgactt ttgtcaaagt cccatcgttt tttatctatt tgtgttctaa acatttcttt
     5941 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
     6001 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
     6061 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
     6121 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
     6181 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
     6241 atcagctgat tattcctcat gtattaaaag atgtagaatt acccgataca actacacatt
     6301 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     6361 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     6421 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     6481 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     6541 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     6601 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     6661 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     6721 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     6781 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     6841 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     6901 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     6961 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     7021 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     7081 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     7141 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     7201 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     7261 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact taataaaaaa ggaagaatat
     7321 tcatgtaaac gattttattg tttttttctc ttttatacaa aaagggtcat aatttcaagt
     7381 aagcggatga attcatgagg attttttgca tactgtctct actatactta ggtcactaag
     7441 aaaaaggagt gatttattat gaaaaagaac atgattggga cattattact ttcaacactt
     7501 cttctaggcg gcttggcaac accagctttt gctgagggtc aagcgacttc aaaaggagat
     7561 attaccttta ctgaaccaac aaataccgtg gagcctctta accctactga tccttcaaaa
     7621 ccagtagagc cagcagatcc ggaaaatcca gcaactggcc agacaggtcc tttaacactg
     7681 gatgtagttc ccgaattgcc atttggtaca cacgagattg aaagcggaac aaaaacgtat
     7741 caagtagacg cttcaaaaaa cgatacacct tatctccaag tatcagatcg tcgaggtgta
     7801 ggtgcagatg gccaagcaca agggtggaac gtgacagttt cggtttcaga ttttgtaaac
     7861 ggttcacaag ttttgcaagg cgctgaatta gattttggaa catccactgt taaaagtacg
     7921 tcagataacg aatccactgg accaactagt caaactgtaa ctgggttgag taaagcaagt
     7981 gcagcgacac caatatttac agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt
     8041 tatgatccag caaatatcac actgaaagtg ccgaaagcag cagctggaac attcacggct
     8101 gatttgactt ggaacctcgt ggctggccca gtagcataga gtatgaaata aattcaacaa
     8161 actgcggtga aaatactata ggtgaaggat tttttaccgc agtttttgtt gaaaggatgt
     8221 ggtggacaat gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt ttaagtctat
     8281 ttttcatttt tccgtcaatc agccaagcaa aggattcagc aggagacttt ggaatcaaac
     8341 cggtatttcc agagaaccaa atagataaag caatcggtta ttttgattta ttagtggcac
     8401 cggaacaaaa tcagacacta gaagtaatta tcagcaattc ttcagatgaa gaacgaacat
     8461 ttgaggtctc tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa
     8521 aaaacccaac gttagatgag acattgccgt ttgatgtccg tgacgttttg ctcattgcga
     8581 aaaaggaaat caatgtatca gcacatgcag aaacaactgt acctatagaa gtgaaaatcc
     8641 cagcaaaaag ctttaaggga cgtgtattag cgggtattca tgtttcacct aaagaagaag
     8701 ctgaaacaga aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct tataatctcg
     8761 cagtcgtttt acaagaaagt caagagacga tagaaccgga tctgaaactt ttatcaggag
     8821 atttggacga ggtcaacgca aagccaactg ttcagcttcg tttccaaaat cctcagccta
     8881 ggattatcag caatttgata tttacgagca agatctttta tgaaaatcag ctttatattg
     8941 aaaatacgtc taatgcattt ttagttgcac caaattcgaa ttttcacttg aatcttgatt
     9001 tagctggtga taaagcaaaa gccggcgatt accgcgcaga aatcatagca aaatcaggcg
     9061 acagtaatga atggcgattt acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag
     9121 taaatgaaaa ttccgttttt gcagtacaag aacaatcttt cccatggctt tatgcaggat
     9181 tggttgctgc agttctagtg ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa
     9241 agaaaaaagg agaaacaaca tgatgaggtg atttcgttga aaacatcgat cttattggga
     9301 gggattattg caagtttgct gttcttcagc tggcaagagc cagtccaagc agataccaaa
     9361 agttcaggta cgattacatt ttatgatcta agtccaattc cgcatccaag tggttcagtc
     9421 aatggcgcat caaaacagca gacacagcta ccgatttata aagaatctaa tcaacatggc
     9481 gtattcccgg agacgaatga acaaaagatc cgttttcttt atttgatcat tggtatagaa
     9541 ttatggcttc ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga tgaacatgtt
     9601 gctacaaaat aaattttcat tttttcgctc cttctttttt acttcgcttt ttgttttaag
     9661 tatatttatc ttttcacctt cggcagaagc tgttggagca acgcctcctg ccatttctta
     9721 tgatccatac acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg
     9781 cgggaagctt gtccaactaa ctggggctaa ccaattgcct aaattgactg ctacttattt
     9841 gaacgatggt tcaaatgatc cttggaaagg tggcacggtc cgttttcatt gggaaagcca
     9901 tgtgaaacta acaaatgtac ttgatttggt gaatatcact gcacaaggat ttgcatttgt
     9961 ggcgatcatc aatttgccgg aacatgtatc aagcagtgat gtattaaaag caattgactg
    10021 gaacaacgcc tacttgtata ttgccggtgc ccccatcaag gttactagcg gaagtttatt
    10081 atcagcagga agccatacct tgcgttttgg gatggggtcg tgggatacaa atactctatt
    10141 gacagatatt attgctatta ttacgcgagg cgaattcaac gtggacgata tcccgcttca
    10201 atttgatgtt acagctaatg tcgcgaagat gacagaaaac ggagataaat atgatacgag
    10261 tccaaacaaa attttgacca agggtaaatt acaaccttct atgtctaaaa aggccgtttt
    10321 ttctgttgat ttttatgatt cgaataatat tatccaagat cgtatgttca attcgatttt
    10381 agctccagct gcaggtagat tttatcctaa attaactggc ggaaacttga atccaggagc
    10441 atatgcattt tctgcaagtg cagagataaa tacgtggaat agttacatct caccaagtga
    10501 ttcaacagga aaatataata catttgctga taccacagaa tacgtggatg gaaataacca
    10561 gaaccttcca ggctctacca atagtcggac attgacgatg gggctgactg aaggacgttt
    10621 tgatcagttg ccatttagtc gctttgatcg cgtagtcaac tattttacag gaaaaaacgt
    10681 tacacaagga gcaatactga ctcatactcc agcaagcaca agtggattga atcagacagc
    10741 caatatcgtg tacagtggga aagatgcgca aggcggattg ctttctcctg ttgctttaaa
    10801 ggtattggag cagtacaagc tagacggaac ggttttacca acaaacttaa cagccgatag
    10861 tcagtgggga aagctccttt cttctgcatc gtaccaagtc acggccaatt ggtcagcaac
    10921 tgaactaaaa aagggttcga tccattacag gctttattcg aaaggaaccc aaaaattagc
    10981 gacaggatgg acagatcaac ttttccaaac gatccaatca aataacgggg gtgtaaatac
    11041 agctacttct acactacctg cattggcttc agggcaatat tattttgatt atcgcttggt
    11101 ggacgatgta cttactaaca tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc
    11161 tggaatcacc actcttcctc aaataacagt ggctaatttt ccatctgtga gtgcagatag
    11221 tcagctaaaa aaccttagca gacaacctga gacaggagat cccctgtctg ctctatcagg
    11281 agatacgatt caagaaaatg tgacattcac gttaacaaaa tctggggatg cgataaccga
    11341 caaaaaaatc acgatctcac ttcctgaaaa tactacttat atcaatggtt ccttaaaatt
    11401 aaatggtaca actatcgcag ataccggcat tcaacaagga gtttctgtcc cagcagatct
    11461 attgagcaaa ataggagata ccattcatct tacttacaac tatcaattaa atactgtaga
    11521 tacttcttta caatccgtca gcatactgac aaaggcagct gtactttcca gcaatattac
    11581 attagcagat ggtgctaagc ttcctaaccc agttgtccaa acatctgcta aaacaattct
    11641 tgtccccaaa caagaattaa cattagttaa tgtaccagat gattttacat ttggtaacga
    11701 tctccccaaa ccgttgaaga caagttatta tgaatcaaaa ggcgattttt cttttgatgt
    11761 gcgggatact cggcttccaa gcacgtctcc ttggcaactg acaggtacat tgacttcttt
    11821 atttaagaat gatcaaggtc aagaattaag cggaacaaaa ttatacttca atcattctgg
    11881 atcaaagcaa ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg atggaacagc
    11941 taaaggagag gttctcgttg actttcccga taccgatggt ttactcttag aggtaaatag
    12001 cagtacgaat gctcaacctg gcgcaacgta tcaaggcatg gtcacatgga aactgacagc
    12061 tgggccaacc agctaagtga ttagaatttg tatggatctg tgataactgt cacagatcct
    12121 ctttttcaag gatgagaagg aaacttctgc tgttaaaatc agaaatgctt gaaggagtgg
    12181 acttggaact tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac
    12241 ttttattaga ggaaaaagga gaggtttccg ttaataacgt acttacccgc ttatcagttg
    12301 atcgggctac cttgaaagaa gatctgtttt atttacaagc taacttggaa aatatcaaac
    12361 aattggaaat tgttatccaa gaagggaata ttttattaat cagacatgga aatatagcag
    12421 cagtcgatgt ttattatcat tgttacttgc gctattctac aaaatatcag atacttcttt
    12481 atctgttgga aaatggctca tttgatcggc agaaacttgt catggaactt aatatcagtt
    12541 cggctacttt gtctcgcaga gtgaaggaat taaatgattc tttaaatgag tttcatctga
    12601 aaataaaaag cgggcggctg accggtccag aaagtcaaat tcgttatttt tatttccaat
    12661 tattttggtt tggacgtccc tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa
    12721 acactttttt agatctgctg caaaaagagt ttagttttgc tattacagaa gaaggacgat
    12781 tgaaatttga aatatggctt tccattatga agaagcgaat gcgctgtacg aaaaaatcca
    12841 aaccagatta tagcaatctt cctgttggat tgtttcaaac gacacaattt ttattggacc
    12901 tccatacatt tttgacccgt tacttctttc aatatgcctt tccttggaat caaaatgaaa
    12961 cgttggtttt ttatcttttc atgatcagta actttacatt agatacacat aactcgcatg
    13021 tccaggagtt tttgataagc gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa
    13081 cgtttaaaca atcgatagaa gaatatttct ctttttatgt ttttccagaa gagatttcgc
    13141 aaaagattgc gttgacagtg atgcaaaatc acgctcattt tgcctatttt agaggatgga
    13201 tgaatatttt tggccaagaa ggattaaaac agcggttaac tgaagtagaa gataaacaat
    13261 tcctggtttt atgtgaaaga ttggtcagac aaacatttgc tattgttgat tttactgaag
    13321 tagacgagga aaatgcccgc tttgaattat ttgctcgcta tgcatctatt ttacggacgc
    13381 tgatcaagca gttagaatta cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa
    13441 tggcggatat cgtgaaagaa atcatccagg aacaattgct gaatacgatc aaatatgatt
    13501 tagtcgaata cgagaaagaa aagcagtatg atatcatttt gactagccaa ttgaaagagt
    13561 acccgtcaca gaagtccgct aaagtttttg tattcactag caacgaatat aattatgatt
    13621 tcccctattt gaaccaattt ttaaaagaat gttatttaga aaaattgaat ttaaggatga
    13681 ataaaacatg aaaatcgtga tcattggtgc ttcccatgca ggcatcacag ctgccctaaa
    13741 tctaagaaaa cttcaaccga aagcagaagt tttactgatc gatgaagatc ataaagatgg
    13801 actaggctat gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac gtagtttagc
    13861 tgaagttgcc cataacatgc ggactcttca agaaagtggg gcaaaactga tcaccgaatg
    13921 gagagtaact gaacttgatc cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa
    13981 aaacgagacc ataacctatg ataaattaat cgtagcgaca ggttcctctc ctgtcacctt
    14041 gtataaacaa attgaagctg aaaatgtcta cacctataaa aatctagtgc aatctaagca
    14101 agtacttgcc gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg gttacattgg
    14161 attggaatta gcagatgctt tgagaaataa aggatacatt attcatctag ttgattatat
    14221 gccgaatgtt ttatcaagat atttcgataa ggatatgatc aacagttttc aaaatcagct
    14281 gcaaactaag caaataaatt tttatccaaa tgaattcttg atcgattgga aaaaatcaga
    14341 agagaaagta gtatccgtac aacttctgtc tcaaacaata aaggcagata tggtcatttt
    14401 ttctgcccaa actcgtccta atacgacatt gttaaaagaa aaagtggctt tgtatgagga
    14461 tgagactgtg atagtcaatg aatatctgca gacaagtgat ccggacatct atgcgattgg
    14521 agatatcgtc ccagtcagtt ttgacaaaaa taaacggcat ctctttttac ctttagttac
    14581 tcgagctgta catatggcaa gagcagtagc gctaactttg agtggtcagc caaccgctta
    14641 cgacctcaga caaaagataa cagcagcagt cattatagac tactttttag gaactgtagg
    14701 gctgacagaa gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt caggagaatt
    14761 tgatctattt ccacaatacg acgaagaaaa caaaacagta aatgcaaagc tgatctacca
    14821 tccggataca ttagaaataa ttggcggaca gctgataagc caagaattct tattatcaga
    14881 tttgaacctg cttgcggata tcgtgaaaca caaaactacg attccacagc tagcagttga
    14941 ggattttgga tttctgtctg aatacacctc tcgttttcac tacctcaatg agttagcttt
    15001 taaagtacta gctaagagag caaatagcaa tagagtagat aaaagactgt gagaaatatc
    15061 acggtctttt attgtgtggg gatataataa atattaatca atcgagaata aaaaattcat
    15121 ataatgaaat agcggaagaa ttttttgagc ataatattcg ccaaaaattt atcaagtgat
    15181 aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat
    15241 gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc
    15301 tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca
    15361 gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg
    15421 atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt
    15481 gcccaaacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt
    15541 agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct
    15601 tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc
    15661 ataggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg
    15721 gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagaa
    15781 atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga
    15841 ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat
    15901 gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc
    15961 gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat
    16021 atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg
    16081 gcacaaacat tcattttatc atttgttggt attcttcttg gtctattagg aacagtggga
    16141 acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc
    16201 attagcttgt tgatgttagt agtagctgtg ttaggcgcac tattttctgt tcggacaatc
    16261 gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagaacatg aaagcaatag
    16321 aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag
    16381 aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag
    16441 gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat
    16501 ttatcaatga tcaagaattc agtaatcaaa aagaaagcga acgcgttaaa ctacgtttta
    16561 aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc
    16621 agttattatt agtagataag attaaacatg aacatcgtca aaaagaagca aacgaattat
    16681 ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag
    16741 aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag
    16801 atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa
    16861 aagaatcaaa agacaaaaat aaagcaacga tcatggttac tcacgataca cggctgattg
    16921 acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa
    16981 aaagcaggtg gaaaaatggt ccatcgatac aacaaagata cacagcaaaa aattttgaaa
    17041 gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag agaaatcgca
    17101 caaaaagttg ggatcaccca gcccaatctc tatcattatt tcggtgataa agaaaaacta
    17161 tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt
    17221 caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag
    17281 acacatctgg tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa
    17341 acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc
    17401 ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat
    17461 tttttcttag tgttagcacc ttacatcact cggtcgaaaa gacctgtgga agaaccgcta
    17521 aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt
    17581 tatataggga gaaaatagtt aggtgaagaa gcaagatatg acgaagatcg ttatatcttg
    17641 cttctttata taggtagttt ttcataaaga caaatttgtt attctctcat gattatcagg
    17701 gtatattttt atttattatg tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa
    17761 ttaattgttt gtagaatgaa ggaaactatt tattgggtag atgaatcacc acgaatagtc
    17821 aaaaggagat ttcaggatat gccaaaaatg aaaaaaatca ttttagcagt aatgttattt
    17881 tctacggtta tatcttttac agcccctttt ttgcttactt aagctgattt taagttttgg
    17941 cccttagtct ggatctacga ggtgagagtc aagaatgaaa aaaataattg gagcttttgt
    18001 atttgcaatc attatcatta ttatctgttt tcaaatagat gtttttggtc aaaaaaagat
    18061 agttggacaa acggtcagca gtacgaacag tacggttaca aagacaaagg aatcatttga
    18121 tgatacagaa caagagacat taatcaaaat cggaaaaaat gaccgtacac tatatacaga
    18181 tgactcgcta actaccccac ttgcaactat aaacggcgga gagctggcgg agtttctttc
    18241 tgaaacaaaa aatgcttatc agatcaagac aaatgatggc tatactggat atttagccct
    18301 cgaagatgga accaaagtca caaagaatat acaaacaaaa ccggaagagc tatctgatgc
    18361 agtgattgta ttagatcctg ggcatggcgg aaatgatact ggggcattaa gcaatgatga
    18421 acaaacagaa gaaaaagata taacattaag tacagccata aaagtaaaaa aggcgctaga
    18481 ggatgctgga gcaacggttt atttgaccca tactacagat gaattagtac agcttggaga
    18541 tatatgcgac tacagtgaag aaaaaaaggc agatgtcttt atcagtttac acgcagactc
    18601 tacggaatat gcgaatgaag caactggaat cacgacatat tattactatg gacaagagga
    18661 aacattagca cagacaattg ctgactcctt cactgatctg cctctggatt caagaggaat
    18721 ctccactggg aactatcaag ttcttcgaga aaatcttcaa ccttctattt taatcgagat
    18781 gggttatatg aataatgata gtgatttaaa ggaattagta acagacagtt atcagcagca
    18841 gattgctgca agtttgacac aagcattgac cacttatttt cagtaataaa taagcccagc
    18901 caaacaaaaa tagctcctca tatttttcgt ttggccgggc ttattatcgc agatgcaagc
    18961 ttttgaacac cgtttatttt tttagtagct tcaaagcttt ttctgtattt gcaaagtgaa
    19021 gtttggcata tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt
    19081 ggtcagattt cgttccagct cctgatggaa gggtgattcc agcttcagct gatgctttgt
    19141 gtaactcttc caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat
    19201 attgttctcc tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg
    19261 catatttttt aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt
    19321 ctggctcgat tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc
    19381 gaacggcatt gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt
    19441 tataggggat ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact
    19501 cttttgaatc tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag
    19561 cagcacatac tgttagcgga ccgaaatagc tgccatttcc gacctcatca ctacctaaaa
    19621 cggataattg actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt
    19681 tatcctgtgc ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca
    19741 cttttccgga tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat
    19801 aaggaatctg tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt
    19861 cgtttgttgc tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac
    19921 tataccgcaa ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa
    19981 agtttatcat ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca
    20041 acttcatggt acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca
    20101 cgaaaaaaca cgctacaaag cggtgattgc caaccaaaca tatactatta tcggacggga
    20161 aaccaagcat catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa
    20221 acaattatca ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct
    20281 ttcggatcaa ttgaagaaac aagaaagaat cttggagctg gaagaagaaa cagcagaatt
    20341 aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat
    20401 cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa
    20461 tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc
    20521 ttctaattaa gatcctttta tcaaaataag aaaaatttaa aaaaagttag aagagaaagg
    20581 aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt ttttctggag
    20641 cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg tcatttattg
    20701 ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt ccatacccag
    20761 ctgtcacgcc aacatcaaag ttagcatttt ttgatcaggt atttgccttt cacctagatg
    20821 aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg ctgcttaccc
    20881 gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga caggtagact
    20941 ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt ttgattttgc
    21001 agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag catagtttcg
    21061 ctcgctttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag ctttgggtaa
    21121 cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg tacagagtag
    21181 attatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat agtgaaagat
    21241 aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa gtcaaacaga
    21301 tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa ttggtaccag
    21361 tgagcgagga acaaacgatc acaaactggc ttcaagaaac tgaggatgga ttgaaagtgc
    21421 aacgtctgcg aggcgggatc cctataccta aacttgaaaa tatccgtccc catatgaaac
    21481 ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc agagtattgt
    21541 ctacgacaag cgaattgaat cgtttcattg atgatcttta tgacagtgaa atcgaatttg
    21601 cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc cgccgtttaa
    21661 aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag ttaaagagca
    21721 taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat gggatcgtct
    21781 gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat gaccgctacg
    21841 ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac gatcaaagtg
    21901 cttcaggaca gactttattt attgaaccaa aacaagtggt ggatttgaat aatcgtttgc
    21961 gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa ctttctgctg
    22021 aacttgttcc ccatcgtcaa gaaatcatcc acaatgctta tgtgattggt aaaatggatc
    22081 tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc tatcgtgccg ggaatcagtg
    22141 aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag gaaaaagtag
    22201 tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca ggacctaaca
    22261 caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg gggcaagcag
    22321 gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa gtatttgcgg
    22381 atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct catatgacga
    22441 acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt gacgagctcg
    22501 gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta gatgatcttg
    22561 gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa gtttatggat
    22621 ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca ttaagtccaa
    22681 cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc tctagccgat
    22741 taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat gagagccaag
    22801 atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag accgaatatc
    22861 tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg aaagaagctt
    22921 acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa aaagcaaatg
    22981 aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt cgtaaaatgc
    23041 aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca aagacccaat
    23101 tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag aaggcgaaag
    23161 aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc caaagaggga
    23221 cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta aaaatgaatg
    23281 tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa cgagttatcc
    23341 atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt cgaggaaaac
    23401 gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt ctagctggtt
    23461 atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag ggaattactg
    23521 attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca aaccaaggcg
    23581 gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc agatggttcg
    23641 ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg tctttgatat
    23701 gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac ttgtattgat
    23761 tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt tggatcagtt
    23821 ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg atgaaaatcc
    23881 agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga aaaaagatgg
    23941 tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc aaatgatcga
    24001 tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa tccagttccg
    24061 tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt gagtataatt
    24121 acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta attgaatatg
    24181 aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt cctgatcagc
    24241 ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc aaagccaaaa
    24301 tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa acggaacggc
    24361 ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc gaagcattgc
    24421 tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg ttaaaggatg
    24481 ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg gtgattactc
    24541 gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc ggtgcggcga
    24601 atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa cctagtcaga
    24661 cgaaggagcc ttgtctttac tatcatttgg gacaatgtct gtgtccttat tatttcgacg
    24721 tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta aacggaggac
    24781 acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa aacatggagt
    24841 ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc atgactcgcc
    24901 aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg attgataaag
    24961 gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa cgagatgttt
    25021 caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc ggtcagtttt
    25081 atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg atcgacaagc
    25141 ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga gaaaagaaaa
    25201 aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg tttgatctga
    25261 ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac gcgatgaata
    25321 tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg acgaatcctg
    25381 tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat cggaaatata
    25441 aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc atctatcgca
    25501 gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg atcgatggag
    25561 gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga atcgatattc
    25621 ctattgctgg catggcaaaa aacgataaac ataaaacaaa tgagttgcta ttcggtacag
    25681 atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc cagcgaatcc
    25741 aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt aaaaacagtt
    25801 ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa atgctgctta
    25861 aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta caagaaatcg
    25921 gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa tgaggaaagg
    25981 gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa gagcgtttac
    26041 gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat gtgatttctt
    26101 gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt gggaactaca
    26161 cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg ctaattggcc
    26221 aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg acacaagacc
    26281 aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat gtcatcggtt
    26341 atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa ctgctttatt
    26401 ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac ttttggatgg
    26461 aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct aaaggaaatc
    26521 agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc attttggatg
    26581 agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga atattggaat
    26641 tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat gtagaaaaaa
    26701 tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga aaagtacatg
    26761 agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta agccaacagg
    26821 aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat acgttagaga
    26881 ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact cagttcggct
    26941 acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa ttgaaagcag
    27001 gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa aaatgttaag
    27061 tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat catttatgta
    27121 gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt ggctgataat
    27181 gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt caaagcagtt
    27241 gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc gtatttagta
    27301 gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc attaggacaa
    27361 agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct tcgagcgagt
    27421 caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag tttgactcga
    27481 caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa ctcgacagtc
    27541 caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt gacgtatgcg
    27601 cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga agtgatcctg
    27661 tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt acttgttgca
    27721 ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca attcaaagat
    27781 atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat tggaccattc
    27841 ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt tttagcagct
    27901 ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt gccagtgact
    27961 tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga tccaaacaat
    28021 atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat gccggttcgc
    28081 ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt acttgtagcc
    28141 gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt actcgtttat
    28201 agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag gaatgaacga
    28261 aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt gttcccagct
    28321 tctttttgta tagacctatt ttatttattt tactatattg gatatatatt cattgaaaat
    28381 agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt taatggaccg
    28441 ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca cggaaatcat
    28501 caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga gtgatataca
    28561 tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa
    28621 gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat tcactatttt
    28681 tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag ataaatcgca
    28741 gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata cccggtcaaa
    28801 agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga
    28861 tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac ttgttttgat
    28921 gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat ctgtcatcga
    28981 tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca aagtgatgga
    29041 tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata ttttgtggat
    29101 tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg cagcacgcta
    29161 taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat tgttttcttt
    29221 tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta aaaatctatc
    29281 cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc ttcaattcaa
    29341 atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc gtaaattgct
    29401 ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct atgagttgaa
    29461 aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg aagaggaaca
    29521 aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc aaaaacatat
    29581 gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tgctgtttca
    29641 tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag accaatctta
    29701 tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg caaaagatct
    29761 gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg ggccattttc
    29821 gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca cagagcctca
    29881 tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt ggttcagtga
    29941 aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta atgggcacac
    30001 gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag tatttgtcat
    30061 tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt attcactatt
    30121 atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta ttgaacaaca
    30181 atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc aactaccacg
    30241 caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta cccagctcaa
    30301 aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat tgttccatag
    30361 actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac ggtgttcttc
    30421 tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg ttttttgaaa
    30481 ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat aaacggtgtt
    30541 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa gccattttca
    30601 caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc agaaagtcta
    30661 ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta cacggtgggt
    30721 aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc tttgaaccgc
    30781 ttcaatgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa gatctttgac
    30841 tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc ttaacaaact
    30901 gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct gttgttcatg
    30961 ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt ccaaaacgac
    31021 attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt tcaatgcttc
    31081 caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta gctttggatg
    31141 aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa taagtttgtc
    31201 gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa taagaggcaa
    31261 tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca cttgatttcc
    31321 ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga ttggtttcat
    31381 ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt aaactatgta
    31441 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaaatttcgt
    31501 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
    31561 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
    31621 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
    31681 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
    31741 gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact gcggcagcag
    31801 ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac aaactatcga
    31861 caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga attagaaaaa
    31921 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agatcaatat
    31981 caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga aacttatggc
    32041 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
    32101 acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa atagaacaaa
    32161 caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag aagcaaaatt
    32221 aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca tacaacacca
    32281 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagattt
    32341 ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc ttgatttaaa
    32401 agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg aagaatggct
    32461 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
    32521 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac tgagtaagct
    32581 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
    32641 tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg ggttcaataa
    32701 agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca agcagcaatt
    32761 atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt gcagtggcta
    32821 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gagaaaaaat
    32881 aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg tagatgcaga
    32941 agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag atatatacgt
    33001 cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
    33061 ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
    33121 agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
    33181 cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc aaaaactact
    33241 tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct cgataccatc
    33301 gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa aagaactgtt
    33361 tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
    33421 tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg cagcaaagga
    33481 aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct ggcaagggat
    33541 cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata cagcatttgt
    33601 ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg ttccgattat
    33661 ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt acggactgac
    33721 cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta tttcaggcgg
    33781 aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg ctcgattcag
    33841 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgctaag
    33901 tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt tttatacagg
    33961 aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcacaa
    34021 gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact taattagtca
    34081 aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt tttcagggat
    34141 cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga caacaagtca
    34201 acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggtacgc aaggtttgta
    34261 tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt cagttgtaca
    34321 aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt tttcaggtat
    34381 tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac ctgaacatgc
    34441 tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag gtttcgttat
    34501 ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
    34561 gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc gttagaacaa
    34621 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt tgaaacagaa
    34681 catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg tcagctaagg
    34741 aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttaaga
    34801 cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga acaaatccag
    34861 tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt cattgctgga
    34921 gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa tgaacttccc
    34981 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
    35041 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
    35101 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
    35161 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
    35221 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
    35281 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
    35341 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
    35401 cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg acaaatcaaa
    35461 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
    35521 ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata cgagaagtac
    35581 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
    35641 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
    35701 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
    35761 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
    35821 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
    35881 atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta aagcttgaac
    35941 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
    36001 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
    36061 tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg gtgagacaag
    36121 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
    36181 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
    36241 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
    36301 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
    36361 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
    36421 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
    36481 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
    36541 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
    36601 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
    36661 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
    36721 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
    36781 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
    36841 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
    36901 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
    36961 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
    37021 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
    37081 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
    37141 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
    37201 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
    37261 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
    37321 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
    37381 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
    37441 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
    37501 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
    37561 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
    37621 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
    37681 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
    37741 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
    37801 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
    37861 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
    37921 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
    37981 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
    38041 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
    38101 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
    38161 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
    38221 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
    38281 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
    38341 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    38401 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    38461 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    38521 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    38581 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    38641 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    38701 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    38761 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    38821 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    38881 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    38941 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    39001 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    39061 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    39121 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    39181 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    39241 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    39301 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    39361 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    39421 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    39481 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    39541 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    39601 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    39661 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    39721 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    39781 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    39841 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    39901 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    39961 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    40021 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    40081 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    40141 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    40201 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    40261 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    40321 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    40381 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    40441 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    40501 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    40561 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    40621 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    40681 aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    40741 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    40801 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    40861 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    40921 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    40981 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    41041 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    41101 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    41161 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    41221 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    41281 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    41341 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    41401 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    41461 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    41521 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    41581 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    41641 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    41701 ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc
    41761 tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct ttatttatca
    41821 ataggttata attcatattt ttttattttt tcttttacga tacttagtcc agaagctaaa
    41881 atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg cgtacgtgta
    41941 gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt ctcaattatc
    42001 ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg attcgttggt
    42061 ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag acttaattta
    42121 gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac ttccacatct
    42181 aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct ttggacatgt
    42241 actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt cattccagct
    42301 aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt atgttgatag
    42361 taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc tttttcaatc
    42421 acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg attattacaa
    42481 gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat caatatatca
    42541 ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata taactgagcc
    42601 aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc tggagagaaa
    42661 agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga tagagattct
    42721 aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa ttgaaaattt
    42781 gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt actagttgat
    42841 tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag accaaaatca
    42901 atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac attgggagaa
    42961 ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc atatttataa
    43021 gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct ttgggagggt
    43081 aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg ttgaatcaac
    43141 gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa cgggtaattg
    43201 tcataagaat taataggcat tttctccact ccttaaaatt gtataccata caattttacc
    43261 attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc tatgaaaatt
    43321 attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca agtaatgaaa
    43381 aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt
    43441 ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt gggggagaac
    43501 agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta tcatttacct
    43561 tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa agcattgata
    43621 aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc aacagattac
    43681 ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca agcattgtcg
    43741 catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta cgatatttta
    43801 gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa aataacgttt
    43861 ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg tacgttgacc
    43921 tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct
    43981 gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga aacttctcaa
    44041 atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct tatagccatg
    44101 gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt agtttctgag
    44161 aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt tggtctagct
    44221 attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg ggctttaaaa
    44281 tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag
    44341 cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa attgccattg
    44401 aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt gagcttagat
    44461 ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca acagcatttg
    44521 ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact caaagcggta
    44581 atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt attatgccga
    44641 aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt aaagaagcct
    44701 acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct aaattgcagc
    44761 cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg acagcagaaa
    44821 gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc aatgaagatc
    44881 cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga atgaataaaa
    44941 caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt gaattaaaag
    45001 aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc tcgtttattt
    45061 gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta tagctacaat
    45121 ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt ctcattttaa
    45181 ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa ctatcctcgg
    45241 atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg cgcagtgcca
    45301 ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc gtgtaatttt
    45361 tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc atacgcacca
    45421 tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt caaataaaca
    45481 agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc aaatgccttt
    45541 tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg agccattttc
    45601 atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta aggaaatagt
    45661 tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat aaaactgtta
    45721 aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt tgctgattat
    45781 caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc agtaatcgag
    45841 gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga gacgaaagaa
    45901 gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat ttcttcttat
    45961 ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta ttataatcca
    46021 ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga aaagaccaat
    46081 aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg tagccaccga
    46141 tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag tagatcagct
    46201 ttctctcagt ttttttattt attacagtac atattttttg attgcttcag ctacaccgtg
    46261 ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa cggcatttcc
    46321 cattgctact cctagaccag catattcaat catggataag tcgttctcgt tatctccgat
    46381 agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa ctgccgcacc
    46441 tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga ccgttgtgta
    46501 tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt ctggttcgtc
    46561 gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg ttcgatattt
    46621 tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat ctcgattagg
    46681 cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc gtgccatcac
    46741 ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt tacctgattt
    46801 cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt gaaaaaggtt
    46861 caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata aaacgatcga
    46921 aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta cttctctttt
    46981 ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca taatttcctc
    47041 caatatattt taatttattt caatcaatgc gccattacgg atatggctgg caaattcttg
    47101 ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca tttcttttgg
    47161 aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa gctgactaac
    47221 ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg tgcgatgtct
    47281 gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca cagcagtgta
    47341 ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga ctaattttga
    47401 atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa aacgtttagc
    47461 taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag tgcccaagac
    47521 cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa atggcagaag
    47581 taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt gtgcgcgatg
    47641 aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt acacttgcac
    47701 atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac tcatggaaaa
    47761 agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat caaatgttcc
    47821 atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat ctgcatcgat
    47881 ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga tcacactagc
    47941 taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt ctgtttctgg
    48001 agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat gttcaaatgt
    48061 atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag tcaccagccg
    48121 ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc gagaaaagat
    48181 atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac ggctatgttc
    48241 tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta gacgttggac
    48301 ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat agttatggac
    48361 tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg ttgtcataat
    48421 gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc cattgctcca
    48481 gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca gaatacagct
    48541 tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt tggtcaagca
    48601 gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt gtaccaaagt
    48661 cttcttttaa agcagattga taaaatcgct tcatgacttg cccgcgtttt tcctgattac
    48721 cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc tgagcgatac
    48781 cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa gaatgggtga
    48841 tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg agcatttgct
    48901 cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc aaagaaatat
    48961 tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc actggagcgt
    49021 aacctgcatc tttcagtgtt tggatcccgt cacttagaaa aggagttcga acatccttca
    49081 tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc tggtgttctt
    49141 tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt ttttgataaa
    49201 tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat aaattcatat
    49261 tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt tttctgtaaa
    49321 aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta tgagaagtaa
    49381 aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat tgcgtacaaa
    49441 agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc aaatggtgac
    49501 aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg gcgatgagat
    49561 tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg caggagaaat
    49621 gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc gaacaccata
    49681 cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga aagaccgtcc
    49741 cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa aaatcggcga
    49801 atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga ttttttgtgt
    49861 ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg aaattagtgt
    49921 atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg cgatcttaga
    49981 acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa actatcttgg
    50041 gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact tgaatattga
    50101 cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt atgctatcga
    50161 ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata ctccacgtcg
    50221 ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga tcgattacaa
    50281 tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc tgtttgatga
    50341 cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag aaatcggagc
    50401 agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag atttaagtat
    50461 catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg actctgaaaa
    50521 agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct atcagaataa
    50581 ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc ccaagtaaat
    50641 acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat taagtgaggc
    50701 agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat cgaaaaatac
    50761 cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg tatcacgatg
    50821 aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt tcataaagcc
    50881 ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt ttatcggata
    50941 ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt ggacttattt
    51001 gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga gctccatatg
    51061 attttagata aaatagacag tccggaaaaa gagattctga tcctctactt ttttgaagga
    51121 tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt taaaacgaaa
    51181 atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga agagtgccat
    51241 gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac aagcaattcc
    51301 caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat catttataaa
    51361 agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta tcgtaatgat
    51421 tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag atttacccat
    51481 cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat
    51541 cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa caattcatgg
    51601 attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg aaaaagaata
    51661 tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa tcgtagacga
    51721 taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata gccatgttga
    51781 aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc cacttttatt
    51841 caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga tggccgaaca
    51901 agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag aagggacgat
    51961 agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag agcatcaatt
    52021 agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac cagaatttgt
    52081 cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg atcattagac
    52141 gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa taataagaag
    52201 agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag tattattgct
    52261 ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac cgaagtaaaa
    52321 ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga acaatcaacc
    52381 attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga atcagcggaa
    52441 aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag ccgagatccg
    52501 gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac aacaccgcag
    52561 cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt aggagaaaat
    52621 acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga gattggcaat
    52681 cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac ggaggaagtg
    52741 acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt tgttcgtccg
    52801 ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat catcgaatgg
    52861 tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca gaagatccaa
    52921 gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga aacgattcgt
    52981 gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac gacagttggg
    53041 gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga tcatcggcca
    53101 gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt agttgaacac
    53161 ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa aaaactgtcc
    53221 accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta ttcgtaaatt
    53281 atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga
    53341 atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt aactaaacag
    53401 ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa
    53461 acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca tccaagtttt
    53521 acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg aaaatccatt
    53581 ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt catcttctta
    53641 tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt ctttatttga
    53701 ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga aaatcgttct
    53761 ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag aagaagccgg
    53821 aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga ttcagcaatt
    53881 agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg
    53941 agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg aggaactggt
    54001 ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg ctgttcagca
    54061 gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg accgtagcag
    54121 cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa atgtacaagt
    54181 tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact tcaaacaaat
    54241 gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac aggaattcga
    54301 atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat
    54361 tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc ctcttacgtt
    54421 aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg
    54481 gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca ctacacgaat
    54541 tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga agaaaaagct
    54601 gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga aattggttga
    54661 attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat atcgtgattt
    54721 attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag tcaaaagact
    54781 agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca aaactgttag
    54841 acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat agttaatttc
    54901 tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt ccaactaagg
    54961 agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat attatcgaag
    55021 tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg attggacgtc
    55081 gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa acagatcttt
    55141 tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca actaagggcg
    55201 aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg catcaaaccg
    55261 aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca ggaaacagcg
    55321 aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc ctagaccgac
    55381 tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg gctgtggtag
    55441 ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc cctgcaagag
    55501 gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga atggctgcta
    55561 aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg gcaacttaca
    55621 attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt gaagcgaatt
    55681 gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag atgactgaaa
    55741 aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc tactttttca
    55801 atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg caagatttgg
    55861 aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag
    55921 cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg
    55981 agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat aaggaggaaa
    56041 tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg tctagcattc
    56101 cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt gacagatgct
    56161 aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa cggaacagtt
    56221 gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc
    56281 aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga atgtactggt
    56341 ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa acgcgttgtt
    56401 atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa ccacgaaaca
    56461 ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg tttagctcct
    56521 atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac aactatccac
    56581 gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga cttccgccgt
    56641 gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa agctatcggt
    56701 ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt tcctgtacca
    56761 acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc
    56821 aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt
    56881 tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca aactaaagta
    56941 atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa cgaaatgtca
    57001 tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga tagacaaacg
    57061 tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc gcttcttccg
    57121 ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa cagtgaaaga
    57181 tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga
    57241 cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca actacgttct
    57301 agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga
    57361 taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat taggtaaaga
    57421 agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa
    57481 tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga
    57541 aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg tatttgttaa
    57601 cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg cttcaactgg
    57661 tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt
    57721 agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt ctgataaaat
    57781 cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg gcggaatgac
    57841 ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag aagctgataa
    57901 agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct tgccaatcga
    57961 caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg aaggagacat
    58021 tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat ttgctgatac
    58081 attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa
    58141 ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag gcgcaacaac
    58201 aatcatcggt ggcggagact cagccgcagc agcagaacaa ttaggatttg ctgacaaatt
    58261 cacacacatc tcaacaggtg gcggcgcaag cttggaattg cttgaaggta aagaattacc
    58321 aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct gacgagtaat
    58381 cgaacaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata aattaaggag
    58441 tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac aaaactgcat
    58501 cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg
    58561 attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca gctgaaggta
    58621 cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg ttcactggtg
    58681 aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc ggacattcag
    58741 aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagcg aaagctatct
    58801 ttgcaaatgg tatgacacca atcctatgct gtggcgaatc tttagaaacc tatgaagcag
    58861 gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat ctttcagctg
    58921 aacaggtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt actggtaaat
    58981 cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac
    59041 tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct gtaaaaccag
    59101 aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa
    59161 gcttgcaagc agattcattc ttgtcattgt tagacgctgt aaaataattg ttgaaaattt
    59221 gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc gtatatgagg
    59281 gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat tattactgat
    59341 gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt agaagtatac
    59401 acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac tggtgaatac
    59461 gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa
    59521 gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga tgttcgtgac
    59581 caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa aggtaaatta
    59641 ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc tgactaccta
    59701 gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc aacaccaatg
    59761 atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca agaattcatg
    59821 atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc tgaagtattc
    59881 cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt
    59941 ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc
    60001 gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc tgcttcttca
    60061 gaattctacg acaaagaaaa aggtgtttac gtactagctg attcaggcga aggcgaaaaa
    60121 acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc aatcatctca
    60181 atcgaagacg gattagacga aaacgactgg gacggattca agaaattaac tgacgtatta
    60241 ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca aaaattatct
    60301 gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca aatcggtaca
    60361 ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac agcagttgta
    60421 tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt tgcaacaaac
    60481 gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa atacaaccaa
    60541 ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt gaaatctttc
    60601 tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac tattgaaaga
    60661 tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa atatcagcag
    60721 gtagagagtt tttttgcaac tagcttctgt atatgatgtt tacaaagaat ggtacaagaa
    60781 aagatcatcc ttgaaaaagg aaagctaaca ataactagca tcatataaga gcttttaagg
    60841 atagaacagt agaataggaa tctatgagca agtcctctcg tataaataaa ataggaggga
    60901 agtagcagca tatttttcat aaagtggctc tctttcttca tttttacttt ggttgctcct
    60961 atttgaaaat tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga gaggattaga
    61021 tgaaaaaaga agaaaaaatc gcgattttac aagagatcat ccgaattaaa tcagtgaatg
    61081 gaaacgaagg tgaagtagca gcctatttga ataagctttt ggcaaagcat gacataacag
    61141 gagagattgt ttcttacaga gatggtagag ataatttgat tgctcgttac caaaagggcc
    61201 aatcaggaaa agtgttaggc ctatctgggc atatggatgt cgtggcggct ggtgatgaat
    61261 cgtcttggac ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg tacggacgtg
    61321 gtgctacaga tatgaagagt ggcttggcag caatggtgat tgccatgatc gaactgaaag
    61381 agtcaggaaa accatttaac ggaacagtca aattattagc cactgtcgga gaagaagtgg
    61441 gtgaactagg tggggaacag ctcacgaaag caggctatgt agatgactta gatgccttga
    61501 tcatcggaga gccaaccaat tacagtttga tgtatacgca catgggatca atcaactata
    61561 cagtcacctc gcacggaaaa gaagcgcata gctctatgcc agaccaaggt tataatgcaa
    61621 tcaatcacct aaatgaattc attacgaaag caaatgctga aatgaatcac ctcgctgaaa
    61681 caatcgagaa tcctgtccta ggaaaaacaa tccataatgt tacattgatc agtggcggga
    61741 atcaagtcaa cagcatccca agccatgcgc agctccaagg gaacattcga tcgattccag
    61801 aatacccaaa tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa ttgaatcaag
    61861 agacagacta tcatttggaa ttgatgatcg attacaacaa aatacctgtc aaagctgatc
    61921 cagattctcc tttgattcat tgcattcagc aacaattcag ccagccatta cctttagttg
    61981 gagcggcagc aacaacggat gcagccgaat ttacgaaagc taaccattcc ttcgactttg
    62041 tcgtctttgg gcctggtgtc gttactttgc ctcatcaagt agatgagtat gttgaaatcg
    62101 ataattattt ggatatgatc gaaaaatatc aaggaattat tttatcctac ttagcgtaag
    62161 atttttttaa taaatgaaaa aacagagcca gattagcatt
//
LOCUS       DAZUKT010000005        60585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000005 DAZUKT010000000
VERSION     DAZUKT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.005"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..1081)
                     /locus_tag="ACG816_000312"
     CDS             complement(233..1081)
                     /locus_tag="ACG816_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4919231.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="ACG816_000313"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="ACG816_000313"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4919232.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="ACG816_000314"
     CDS             complement(3582..3761)
                     /locus_tag="ACG816_000314"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4919233.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /locus_tag="ACG816_000315"
     CDS             complement(3808..5631)
                     /locus_tag="ACG816_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4919234.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /gene="helD"
                     /locus_tag="ACG816_000316"
     CDS             5909..8188
                     /gene="helD"
                     /locus_tag="ACG816_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4919235.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="ACG816_000317"
     CDS             complement(8281..8928)
                     /locus_tag="ACG816_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919236.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="ACG816_000318"
     CDS             complement(9107..9826)
                     /locus_tag="ACG816_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4919237.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="ACG816_000319"
     CDS             complement(9957..11108)
                     /locus_tag="ACG816_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4919238.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="ACG816_000320"
     CDS             complement(11325..12065)
                     /locus_tag="ACG816_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4919239.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="ACG816_000321"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="ACG816_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4919240.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="ACG816_000322"
     CDS             complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="ACG816_000322"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4919241.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13676)
                     /locus_tag="ACG816_000323"
     CDS             complement(13026..13676)
                     /locus_tag="ACG816_000323"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4919242.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="ACG816_000324"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="ACG816_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4919243.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="ACG816_000325"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="ACG816_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4919244.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="ACG816_000326"
     CDS             complement(15119..15646)
                     /locus_tag="ACG816_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4919245.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="ACG816_000327"
     CDS             complement(15826..16611)
                     /locus_tag="ACG816_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4919246.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /gene="accD"
                     /locus_tag="ACG816_000328"
     CDS             complement(16604..17473)
                     /gene="accD"
                     /locus_tag="ACG816_000328"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4919247.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="ACG816_000329"
     CDS             complement(17474..18853)
                     /locus_tag="ACG816_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4919248.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="ACG816_000330"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="ACG816_000330"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4919249.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="ACG816_000331"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="ACG816_000331"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4919250.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="ACG816_000332"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="ACG816_000332"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4919251.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="ACG816_000333"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="ACG816_000333"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4919252.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="ACG816_000334"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="ACG816_000334"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4919253.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="ACG816_000335"
     CDS             complement(22811..23035)
                     /locus_tag="ACG816_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4919254.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="ACG816_000336"
     CDS             complement(23088..24050)
                     /locus_tag="ACG816_000336"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4919255.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="ACG816_000337"
     CDS             complement(24060..24500)
                     /locus_tag="ACG816_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4919256.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="ACG816_000338"
     CDS             24806..25771
                     /locus_tag="ACG816_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4919257.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="ACG816_000339"
     CDS             complement(25828..26058)
                     /locus_tag="ACG816_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4919258.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="ACG816_000340"
     CDS             complement(26144..27031)
                     /locus_tag="ACG816_000340"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4919259.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="ACG816_000341"
     CDS             complement(27120..28343)
                     /locus_tag="ACG816_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4919260.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29406)
                     /locus_tag="ACG816_000342"
     CDS             complement(28360..29406)
                     /locus_tag="ACG816_000342"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4919261.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="ACG816_000343"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="ACG816_000343"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4919262.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="ACG816_000344"
     CDS             complement(31297..31563)
                     /locus_tag="ACG816_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4919263.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="ACG816_000345"
     CDS             32042..34276
                     /locus_tag="ACG816_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4919264.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="ACG816_000346"
     CDS             34424..34744
                     /locus_tag="ACG816_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4919265.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34949..35173)
                     /locus_tag="ACG816_000347"
     CDS             complement(34949..35173)
                     /locus_tag="ACG816_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS4919266.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35371..36951)
                     /locus_tag="ACG816_000348"
     CDS             complement(35371..36951)
                     /locus_tag="ACG816_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4919267.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37352..38728)
                     /locus_tag="ACG816_000349"
     CDS             complement(37352..38728)
                     /locus_tag="ACG816_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4919268.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38856..39944)
                     /locus_tag="ACG816_000350"
     CDS             complement(38856..39944)
                     /locus_tag="ACG816_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4919269.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            40116..40550
                     /locus_tag="ACG816_000351"
     CDS             40116..40550
                     /locus_tag="ACG816_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4919270.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40628..41161)
                     /locus_tag="ACG816_000352"
     CDS             complement(40628..41161)
                     /locus_tag="ACG816_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4919271.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41255..42433)
                     /gene="mutY"
                     /locus_tag="ACG816_000353"
     CDS             complement(41255..42433)
                     /gene="mutY"
                     /locus_tag="ACG816_000353"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4919272.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42426..43223)
                     /gene="recX"
                     /locus_tag="ACG816_000354"
     CDS             complement(42426..43223)
                     /gene="recX"
                     /locus_tag="ACG816_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4919273.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43330..44712
                     /gene="rlmD"
                     /locus_tag="ACG816_000355"
     CDS             43330..44712
                     /gene="rlmD"
                     /locus_tag="ACG816_000355"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4919274.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44886..45464)
                     /locus_tag="ACG816_000356"
     CDS             complement(44886..45464)
                     /locus_tag="ACG816_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4919275.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45786..46256
                     /locus_tag="ACG816_000357"
     CDS             45786..46256
                     /locus_tag="ACG816_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4919276.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46364..47590)
                     /locus_tag="ACG816_000358"
     CDS             complement(46364..47590)
                     /locus_tag="ACG816_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4919277.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48344..48544)
                     /locus_tag="ACG816_000359"
     CDS             complement(48344..48544)
                     /locus_tag="ACG816_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4919278.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48678..49286)
                     /locus_tag="ACG816_000360"
     CDS             complement(48678..49286)
                     /locus_tag="ACG816_000360"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4919279.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49371..49841)
                     /locus_tag="ACG816_000361"
     CDS             complement(49371..49841)
                     /locus_tag="ACG816_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4919280.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49889..50803)
                     /locus_tag="ACG816_000362"
     CDS             complement(49889..50803)
                     /locus_tag="ACG816_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4919281.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50949..51752
                     /locus_tag="ACG816_000363"
     CDS             50949..51752
                     /locus_tag="ACG816_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4919282.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51930..52877
                     /locus_tag="ACG816_000364"
     CDS             51930..52877
                     /locus_tag="ACG816_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4919283.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52945..53238)
                     /locus_tag="ACG816_000365"
     CDS             complement(52945..53238)
                     /locus_tag="ACG816_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4919284.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53266..53931)
                     /locus_tag="ACG816_000366"
     CDS             complement(53266..53931)
                     /locus_tag="ACG816_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4919285.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(54071..54703)
                     /locus_tag="ACG816_000367"
     CDS             complement(54071..54703)
                     /locus_tag="ACG816_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4919286.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54710..55777)
                     /gene="liaS"
                     /locus_tag="ACG816_000368"
     CDS             complement(54710..55777)
                     /gene="liaS"
                     /locus_tag="ACG816_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4919287.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55774..56505)
                     /gene="liaF"
                     /locus_tag="ACG816_000369"
     CDS             complement(55774..56505)
                     /gene="liaF"
                     /locus_tag="ACG816_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4919288.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56674..57153)
                     /gene="greA"
                     /locus_tag="ACG816_000370"
     CDS             complement(56674..57153)
                     /gene="greA"
                     /locus_tag="ACG816_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4919289.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57289..58464)
                     /gene="mltG"
                     /locus_tag="ACG816_000371"
     CDS             complement(57289..58464)
                     /gene="mltG"
                     /locus_tag="ACG816_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4919290.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58668..59837
                     /locus_tag="ACG816_000372"
     CDS             58668..59837
                     /locus_tag="ACG816_000372"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4919291.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(60104..>60585)
                     /locus_tag="ACG816_000373"
     CDS             complement(60104..>60585)
                     /locus_tag="ACG816_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919292.1"
                     /translation="YSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQL
                     LLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATV
                     LKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        17613 a        12679 c        10195 g        20098 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatctga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag aacacttttg
    34801 tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg gaaataagct
    34861 gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc ttggagttaa
    34921 acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt ttcttttgta
    34981 cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt tcctctttat
    35041 ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct tttacatcta
    35101 ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca cgtctttgcc
    35161 attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa aataattttt
    35221 atattttttt gattagacat ttctatgatt ttaatatagt aaaaagagcc gtcacggaaa
    35281 atccgtcacg gctctaaaca aaatatgaga ggctgttttt gtttggctat tcttattact
    35341 tgatgcaggg cagcttgtgt caagacttct ttacatcaaa ctttttaatt ttacattggg
    35401 atatttatcc gcaaaccagc gttctgcaaa ttggttctcg aataagaaca acggttggtc
    35461 gaaacggtca cgagccaaga tattgcgact tgaactcatc ttctcatcca aatcttctgg
    35521 atcgatccag cgagcgattt tgtgtcccat tggagacatg attacttcgg cgttatactc
    35581 atttaacata cgatattgga atacttcgaa ttgtagctga ccaacagctc caatgatata
    35641 ttcttccgtt agatatgttt tataaagctg aatcgctcct tcttgaacta attgatagat
    35701 tcctttgtgg aaggattttt gtttcatgac attttttgca gtcactttca taaacagctc
    35761 tggagtaaat gaagggagtt cttcataagc aacttttagt tttccttcga acaatgtatc
    35821 cccaatctga taattgcctg tatcatatac accgatgata tcgcctgcta ctgctttttc
    35881 gacgttttct ctcgaatctg ccataaattg tgtgacattg cttaatttca gttttttatt
    35941 tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt ccagaacaga ttcggacaaa
    36001 agcgattcgg tcacgatggg ctggattcat atttgcttgg atcttgaata cgaaacctga
    36061 aaattcttcc tcataaggac ttaccatctg atcatcttgt gttttgtgtc cataaggagc
    36121 tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca ccgaagttag tcaatgcaga
    36181 accaaaaaat actggtgtct gatcgccacg agcaattttt tcttgattaa atgcatctcc
    36241 agcctctttg attagttcta cttcttctaa gacttgttca tataaggaat tttcagttag
    36301 aggatgattt tcgggaagca gtccatcttc atccaattct actaatcgtt catcctgata
    36361 attttccgga cgataaaact ctacacgatt atggtagata tcataaagac cttctagccc
    36421 tttacccata ccgattggcc aattcatcgg ataagattcg atatctaata gctcttctaa
    36481 ttcttctaac aattccaatg gttcacgacc atcacgatct agtttgttga taaaagtaaa
    36541 tatcgggatg ccacgttttt ttacaacttg gaataatttc tttgtctgtg cttcgatccc
    36601 ttttgcacta tcgattacca tgaccgcact atccacggcc atcaaagtac gataagtatc
    36661 ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt actcgtttcc cttgataatc
    36721 aaactgcata acagaactag taacagaaat cccacgttgt ttctcgattt ccatccagtc
    36781 tgatttggca aaattacctg tttttttgcc ttttacggtc ccggcttgac ggatagcccc
    36841 accaaacaat agtaattgtt ctgtaatcgt tgttttccca gcatccggat gggaaatgat
    36901 cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt ggattattca taacctaact
    36961 ccatttcttc cattcaatta tttatatttt ctctaggcac agattgtccg atctcttaaa
    37021 gaaagacaat ttcatatagg gctgtgacaa aagccgtgta gcagtctctt tttccgagta
    37081 aacggcgttc tagagacagc ctatcagcaa taagcccacc caagccaaaa atacgaaaag
    37141 ctgtttttgc ttggctgctc ttattactta atgcaggata gctttttcac aacctttgtc
    37201 aactactaca actgaatcag tatagcatgg attcagcgaa tgaaaaagcc accaaaccat
    37261 aagagtcaaa tatttttttg tcagctgact aaaagaaaag acgtgttgct tgtttattca
    37321 agcaacacgt ctttcataac caagtttcca attatcttct tggctcgtca tcttcgaatt
    37381 cttttctgaa gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc ggatcatgaa
    37441 aaatgacacg gattttaagg attcgagaac cttccatttc ttcagaaacc agcgtaatat
    37501 tatcgacatc aaacgataac ttttcgcctt catccggaat catgcctaat gctgtaatca
    37561 aataaccagc catcgtatct acatcactca tatgcagatc actgtcaaaa gcttcattga
    37621 actcatcaat cagcatacgt ccttggatga tatattcgtg ttcatcgatt ttttcataaa
    37681 gattctcaac ttcatctgat tcatcatcga tttctccgac gatttcttct aaaaggtctt
    37741 ctagtgttgc taacccaacg acgccgccat attcatctaa taaaatagcc atctgattct
    37801 gcgtcttttt catttcatat aacagatcat caataaagac cgtttcagga acgaataaag
    37861 gctcctgcat gattttcttg atatctaaat tatcaaagcc gaatttatgg gcagctttta
    37921 gtaaattctt tgtatgaagg attccgacta ctttatcttt gtcttcgtta taaacaggga
    37981 ttcttgaata gttttcggat aaaacttcat tcacattttc ttccacagtg tcgttgatat
    38041 caatcataaa tgcatccgtt cgtgggacca tgacttctcg ggcaactttc gtatcaagag
    38101 aaaaaacccc ttggagcatt tctaattctt cattattcaa tacgccttcg ttttcaagca
    38161 tataacgcat ttcatctctg gtcatttttg aatcttcatc atcaaaagtc atcggcgtga
    38221 tacgcgccaa taaacttgtg gaagcagaaa gcagccagac gaagggctta gctactacac
    38281 caatcaagcg tatcagccct gaagtgaact ttgcgacttc ttctgattta tttaaagcaa
    38341 tacgtttagg gtacaattct ccaaaaacaa tcgatacata ggtcaaaata gccagcacaa
    38401 tgatatttgc aatatttttt gccgctgtgc cgccacctaa taccggagct aaccgttccg
    38461 ataaagtatt agctagtgaa gcaccagaca aaatgttgac caaagtgatt cctacttgga
    38521 ttgtcgatag gaaattattt ggattctgca atatttttaa aagcttttga gatttcacat
    38581 ctccttcatc agctttttgc tcgattcgat tcttattgat cgaaacaaca gcaatttcgg
    38641 aagctgctaa aaaagcattg atcaatgtca aaacgatcaa taataaaatc tgcgctaaca
    38701 gcgactgact ctcagggtca gcattcataa tcgcattctc tccttataaa attaattttt
    38761 agtttacgat agaaaaaaac gtggcactat agccacgtta aaatggtatc acaaagcaat
    38821 tttctttgca ataaaaacaa gtgtactaaa actacctacg aggtcgtagc aattttcttc
    38881 ttgttttcat tttgcttgtc ttctttgacg attttgatta tataggaaat gatcgtcttg
    38941 cagattgcgt aaaaaggaac acctaagaag ataccaagca atccggcaat atttccagct
    39001 actagaagaa cgataatgat cgttaatgga tggattttca gtgatttccc aattacatta
    39061 ggataaatga tattcccatc caactgttgg acgattagca caaccacaca acaaagtaat
    39121 gcttttaccg gatcattgaa gactgttact agaaatgcag gtgctagtcc tagataaggt
    39181 cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc caaataagaa agcataatcc
    39241 acaccaatta ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca ttcgattgct
    39301 tgtccgctaa tgtaattagc caatgtttta ttcagttgcc ccaataactc cacaatttgg
    39361 tttcttcttt tttctgggaa aaactgttgg atgtttggca ctagtttttc tccgtctttc
    39421 aacatataga acaagatgaa tggtgctgta atgattacta cggttgccga agcgatcgtt
    39481 ccaacgatcg aacctaaact attggatact ccactaagga attgctgaat gattgtcccg
    39541 taagaaatgt ctaattgctc aaaatattta ttgatatcta cttccttgaa caaaggcaat
    39601 tcggctacct cttttaacca gctttctacc tgtttgatga aatcaggcat attagatgcc
    39661 aatgaggaaa tttgctgtac tagacttggg atgacactaa gtaaaatcca aataattgct
    39721 gcaactaaaa ggagcagcac aattgcgaca gcccatattc ttttgatttt tgctttcatc
    39781 aacagcttga caataggatt caataaatag taaagaaagc ctgcaatcag tacaggtgca
    39841 aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc tgatgaaatc
    39901 atgatcaatg cagctatgat caataattca agcgaccaaa acattagttt agactgtcta
    39961 agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta tattcgttaa
    40021 aaattctttt attctttttt tatttttgaa aacttgccat actttttatt tgttttattt
    40081 tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata
    40141 caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac
    40201 agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc
    40261 tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa
    40321 aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg ttaggaaaaa
    40381 tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg attttattag
    40441 gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct tacggcgatc
    40501 cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa aaatttattt
    40561 cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt actgttcttt
    40621 tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca atatagcctt
    40681 ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca ttttctttta
    40741 ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca tattcatcga
    40801 catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca tagtcgatat
    40861 atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa taggaaaccc
    40921 ccttttctct gatcattgct attacattga accagtattt tttatgaaaa taaacaatcg
    40981 ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg tgatcattca
    41041 cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac gttcgatgca
    41101 atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt ggaactccca
    41161 tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat tacaccgaca
    41221 tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta ttgtttcgat
    41281 ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc gaatcaaatg
    41341 catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct gctccctcgg
    41401 ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata tgagtaactt
    41461 ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg agattttgct
    41521 cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc cattcttttt
    41581 tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac atattcgcca
    41641 gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt tgaagagctg
    41701 cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga aaactcgttt
    41761 ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca cactttggag
    41821 aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat tcacctggat
    41881 gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta gaagcttttg
    41941 cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg ccgtcaactg
    42001 caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat ggtcctattc
    42061 ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat tctgacatga
    42121 tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg ccctcccatg
    42181 cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga aaccattcca
    42241 tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc atgatctcag
    42301 agatccagat acgataagga tcacgattat agcgccaagg caggtttctt ttttcttgtt
    42361 cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc catttttccc
    42421 attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat atccaaatca
    42481 aatccttttt gatataagct ttgctttact tttagatttc tttttttagg ttcaaacctt
    42541 aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc tatttcttga
    42601 tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga aaagcctttc
    42661 gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc gtgatttttt
    42721 ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa tgtatattgg
    42781 ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg ctgcgctaat
    42841 ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc cccatagacc
    42901 aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg atcttcttga
    42961 aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa ctgataactt
    43021 aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt gatctcttgg
    43081 atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa tacatcttca
    43141 gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc tttgccttta
    43201 ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc tatcatacca
    43261 cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa aagaaaaagg
    43321 gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt gaaaatcaag
    43381 cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat atttgttcca
    43441 tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc tcgctatgca
    43501 gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc accatgtcct
    43561 gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc tcaattggat
    43621 tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg ctttcgatct
    43681 tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa caaagcacaa
    43741 tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata cgaagcaaac
    43801 tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac acaaaaagta
    43861 atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga tgaacggaca
    43921 aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg ggagttacaa
    43981 gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat ccgtgagatc
    44041 aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca aaaaacgtct
    44101 ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga agaacaaatc
    44161 aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc tcagcagacc
    44221 gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga aacgatcgtg
    44281 gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc caaagaagta
    44341 cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc ggaaagatta
    44401 ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc taaatggttg
    44461 aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg tttggataac
    44521 gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat ctcctgtaat
    44581 gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat tgaatattta
    44641 cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa gatgacgaga
    44701 ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt aataagaaca
    44761 gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg aagagttaac
    44821 aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac agcccttttt
    44881 gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt atcgctgata
    44941 ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt gatcgtttgg
    45001 ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa ttcaaaatca
    45061 ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta atcccaagta
    45121 cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg attaaccatg
    45181 tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct gactcctgca
    45241 ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg cggttcccat
    45301 tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag gtatgcacgg
    45361 cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc gaacaaccct
    45421 aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa acctccttca
    45481 tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc ttttataatt
    45541 ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga agtagacgag
    45601 aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa aacatcattc
    45661 atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc ttacttcaca
    45721 aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag aaaggagtta
    45781 gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga attgtcatat
    45841 taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat tctttgcaac
    45901 ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta ctgtatatat
    45961 tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag cgaaacaatc
    46021 ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct ctatttttta
    46081 atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt ttcttgtcag
    46141 tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg attccttatt
    46201 atttatgggt actattcgca actctattga attattcaat ttatttttta aactaagcgc
    46261 taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt tagatttttt
    46321 gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac ccacttgttg
    46381 ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca gcgtaaggat
    46441 cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct gtttgctgat
    46501 aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa gaataatcgg
    46561 tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc gaatcatcaa
    46621 agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt gtttttatat
    46681 ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct aaaatttgat
    46741 agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg attgtttcaa
    46801 acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag tccgtcgtca
    46861 cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct aaacttaaaa
    46921 ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg atatcgcctt
    46981 ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc aatgtttgtt
    47041 cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc actttgatgc
    47101 caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca taatgatcaa
    47161 taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca ccaaatgcgt
    47221 aggcatgatt taatttatct accgttccat aacctacgat attcgtataa atatctcggt
    47281 ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc atcatcgtat
    47341 cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat agcaaaaccg
    47401 aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat attcgattag
    47461 agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg attccgtatg
    47521 ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga actttttgcc
    47581 atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat ataactgttc
    47641 aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt gtgtaacgat
    47701 tattattttt taaaatttct caaaattctt aactatcata ccataccgtg aaaaagatcg
    47761 aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt ttttccagaa
    47821 aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc acataccttt
    47881 cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc acgacaattt
    47941 cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt ccagctcact
    48001 gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt taatggatag
    48061 atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg acatggtaat
    48121 gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa aactgaaatt
    48181 tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag ttaggacatg
    48241 tcaagtgaac aaatgcattt acttagccaa aaaatctaaa aacttcataa atagacaaaa
    48301 agacacgact ctctaagaag tcgtgtcttt attcaacaaa ttattataat ttagtcactt
    48361 cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa ttctacttct tgaccttctt
    48421 ctaggctctt gaagccgtct ccttgaattg ctgagaaatg aacaaatacg tcatctccgc
    48481 cgtctactgt aataaaccca aatccttttt cattgttgaa ccatttaacc gttccgtgtt
    48541 ccataaatgt atatcctcca aaaaagttct tgcaagtaac cttgcaagaa cattataccc
    48601 aatttagaaa acgctagcaa gctttcgctc ttttattttc caaattattt ttaagactga
    48661 tatgacggag gaaatggtta ttttgctgct tcgaaacgtt tgtttacttc atcccaatta
    48721 ataacattcc agaatgctgc aatataatct ggtcggacat ttttatattt caaataataa
    48781 gcatgttccc aaacgtccaa acctagaatt ggtgtctttc cttccatcaa tggagaatct
    48841 tgatttgcag tagaggtaat agctaatttt ccattttcca ttacaagcca agcccatcca
    48901 gaaccaaatc gtccagcggc tgcttttttg aattcttctt taaaagaaga aaaatcacca
    48961 aaagcttcat taatcgcttc ttttatttct cctgtaggtt cgccaccagc atttggtgcc
    49021 atgatttccc agaaaaatga atggttagca tgtccgccac cattgttacg tacagctgtc
    49081 ttgatatctg ttggaatagc gtccatatca gataataatt cttctattgt tttttcgcct
    49141 aattctgggt atttctcaat tgctgaattt aaattcgtca cataagtatt gtgatgctta
    49201 tcatgatgca gatgcatcgt ttcttcgtca atataaggtt ccaatgcgtc atatgcataa
    49261 ggtagatctg gtaatgtgta agtcataaaa aaattcctcc tcaatgtttt cttaataatg
    49321 tctacattac aaagagtaac aaagagaagg aaattcttca attgttatgc ctagtcgtct
    49381 ttttcacttt gacctacttc ttcttcaaaa acctcagttt ctttaaaaat aaacaacttg
    49441 ctgaaaatat agttcgcaag gataacaata atttgagtga tgattttcgc cacatactca
    49501 ttgctgttca taacattgat caacaatacc attgccccca tgtccaatcc gtaagaaagg
    49561 atacggtaga agaagaactt accgcattca ataagtgcat gtgtaaaatt cggtgatttt
    49621 gaatggaata cccaaagttt gttcgttaca aaagcaaaca atacagaaca aaaccacgaa
    49681 atagaattgg ataaggccat acttaaatca aaatattggc gtgcgagagc aaaactaaca
    49741 aaatttacaa tcgtagccaa accaccaaaa aagagataga ctagtatctc ccaatatcct
    49801 tttgcttcta aatatccttt taactgtcga tactgcttca tgagccaacc taccttatta
    49861 aaaattctta tttatcatac tctatatttc aataactttc atctttattt aaaaaaatta
    49921 attttttttg atcctttgcc tttgtctctg cttggatcgt cacatgtgta ataccatatt
    49981 ttaattttaa taaagaaatt atacgaggat atatcttttc aatctcgctt agtagctgat
    50041 cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc gctatactgc caaatatgga
    50101 catgatgaac atcctttacc ccatcgattg cttcaatttc ttgttccatt ttttcatagt
    50161 cgatagcagg tgcactttgt atcaaaatag cggcagcttt tttgatcacc attatcgctt
    50221 ctatcaatat atatatcgaa atgatcagtg taataactgg atcgatccag tagatatcga
    50281 aaaaacggat caaaattgct ccgataatca cacttaccga agacagtgta tcacttaaca
    50341 aatgcaaata actagatttt atattcaaac tttcatctgc ttcttgtcgt aataataata
    50401 ctgagaacaa attcgcgagc aatccaataa ctgctactgt tagcatcaaa tgactattta
    50461 tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt taaaaacaag gataatatga
    50521 tcaatatgaa tgagttgatg aaagcactca atatttctaa acgctggtag ccatacgtac
    50581 gttttttatt tgcttctttt cgggccattt tttgtgcccc gtaagaaaaa ataattgcca
    50641 atgtatcact aaggttatgg aaagcatctg aaacaagaga caaactccct gacataatcc
    50701 ctccaataaa ttctgataaa gtgatcaccg cattcaaaac cactactcca aatagttttt
    50761 ttgatgatct ctgtggctgt gtaggcacta ttccttcctc cattttattt atattggata
    50821 gtctttagac aagaataaca tttttttgat atttcaccaa tcattcagct gtttccttaa
    50881 agcacttgta tttcacttga tacttcgcta gaattgcaag tgatgacact actagatgag
    50941 gagtacttat gacaacaaaa caattaatta tcagttcctt tatacgattc aaagacctaa
    51001 aggaggcgag aaatgtccca ttttggaaag ccattgtcta tcttttagtt ttgagtataa
    51061 ttatggcttt gccaatctct tttcaagttt ttcaagtctt aaacaacatt caattggatg
    51121 ggcaaaagat tgctactcgc attcctgatt ttacaatcca aaacgggcag cttcaaaccg
    51181 aagaaaaaga aggattcatt taccaaacaa actccattat cttcacattt gatccagagg
    51241 gaaaacgaac agaacaggac atctctactg atttgatggg gaattttgtt agtgtcggga
    51301 tgttaaaaaa caaacttatc atagctcttc ctaatacagg cacgacttct gctttgttga
    51361 acaataacca attagaactt ccctacacca atgaatcttt aaaaaactta acaggtaagc
    51421 agctccgttc ttttttaagt gaagcatcta taccactttg gataaaagtc ctgacattta
    51481 tattttctat ttatcctagt tttctgaatc tgctgatcac tttgctattt accaacattg
    51541 ctgcttttct ctatgcacgc tttcgactaa caaaagctac ttttcttgat tgtttaaaaa
    51601 cattgatcta ttcaatttca ttgcctacta taattgcaac aatcttgatg gtctttcttc
    51661 cttcttttga cactagcgcc ttcattgctt ttgcaggaat ctttattttt gctcaagctg
    51721 tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa gacaactcgt tatttctact
    51781 tcaatagacc cttttagagg ttgtgacatt tttttgtcac agcccctttt ttatttgaaa
    51841 aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt tcttcttgta tgcgctacac
    51901 ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag aatcatttat gcagatcgtc
    51961 catttcgtga tgaatttatc gaagaaatcc atactatagc gcctggttat atctttaaaa
    52021 cagaattaca accagaggat ctacaaaatg tagagatcag tttgggctgg aactcagact
    52081 accaagaaaa actattagcc tcttctacct tgaaatgggt ccaatctatc tcagctggtg
    52141 ttgatcagct accccttgag actttttcaa agaaacatat tttactttca aatggtagtg
    52201 gcatccacag tgaatcgatt acggagcatg tcttaggaat catccttggg tattctcgtg
    52261 gtctttttca agcacaaaag gcacagagtg acatgaaatg gttaggttca tcggtccatt
    52321 accaatctat ccaagggaaa aaaatactaa tcattggaac tggccatatt ggaaaaatga
    52381 ttgcaaaaaa atgcaaagca ttagacatga actgctatgg aatcaataca tccggtcatc
    52441 cagcagaagg aatggaaaag actttttctt taaatcaact aaaggaagtt cttcctgaag
    52501 tagatttcgt agtcaatatc ttacctctaa cggaggaaac aactgggctc tttgatcagc
    52561 atatgtttga acactttgat ccaaaatctg tattcatcaa tgttggccgt ggtgcttctg
    52621 ttaagacaca agatctggtg caggctttaa ataaccagca actttccttt gcagcattag
    52681 acgtttttga ggaagagcct cttccacaag atcatcctct ttggaaaatg gacaatgtac
    52741 tgattacttc ccacattgca ggtttgacac ctgattttca aaagaaatta atggctattt
    52801 ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat caagtaaaat
    52861 tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaaaaaaga gaaggtgaca
    52921 aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg gctcctccag
    52981 gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat ctgcttcctc
    53041 gataaaaaaa agtgtcccat caatttccac tttctttaca ggatgttctg gctgttcaac
    53101 tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt ttccaaaaaa
    53161 acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt gtgcttttgg
    53221 cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt gattcatctt
    53281 atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat cattttcacg
    53341 aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc gtatggcgac
    53401 tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt tgaaaaattt
    53461 aggattggag actcggattt ctgctaaaga atatttatct gacaactcta ggtaatctaa
    53521 aatattcttt gacactaaat tatgcgcaat ccttattccc atgtctcgct cgggatggac
    53581 gacgcgatca gctccgatct tttcaagtac tctggcatga tactcatttt gggcttttgc
    53641 taaaatattt ggtacgccca tttcctttac catcagagta actaagatac tggcttgaat
    53701 atcttctcca atagcgacaa ttacatgatc gaaatttctt atgcctaatg atcgaagtgt
    53761 catttcatcc tgtgcatttg ccacaacagc atgagtggct atattcatat attcattgac
    53821 ccgatcttca ttactatcaa tagccagtac ttcttgacca gcttcgatca gtgttcggca
    53881 gatacttccg ccaaatcgtc caagacctac aattgcaaaa ttttgtttca ccgttttcct
    53941 cctttagtat cttcttttct cagtataaca aagcctttac aaaaacaaaa gcaagcatca
    54001 ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat tatcctcttt
    54061 atcatcgttt ttatttcacc aaaccatgtt tgaatgcata gatagcggct tgtgtacggt
    54121 cttccacttc tagcttagcc aatatattgg aaacatgtgt ttttactgtt ttcagtgtaa
    54181 taaacaattc gtccgctatt tcctgattac tttttccttc agaaatcagc attaaaattt
    54241 cattttcccg attcgttagt tcttcatgta atacaggctc atttcgccga ctcatttttt
    54301 ccatcatttt tgttgtcact tctggttcta atactcgctc gccacgttgc gttgccctta
    54361 tagcatcagc aatttcatgt gcggtagagg tcttcaacaa ataccctgct gcacctgctt
    54421 caatggcagg ataaactttt tcgtcatcaa taaaactggt tacgataatg attttagctt
    54481 ctggccaatc ttttaagatt gcttttgttg attcgatacc atccatttct tccatgacta
    54541 agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt ctcatagcct tgtcgtccat
    54601 tttcagcttc tccaattact tcaatgtctt cttgaataga taaataaaaa gagacgccta
    54661 agcgcaccat ttcatggtca tctactaata aaacttttat catccttatc tatgcctcct
    54721 tcatcaaagg gaccttgatt tctacacttg taccttgacc tttaaaacta atgattttta
    54781 ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa cttcccgttt
    54841 tcgtttcatt catattaaac cctgttccgt cgtcaataat acgtaacaaa agattattgt
    54901 ctattttgtg caaatatacc tctaattcat tggctttagc atgtcttaat gtatttgata
    54961 acaattcttg aacgattcgg aacaagtgat cctcaatgga actagatagt tttacatctt
    55021 ctacatccca tttcagcgaa atctggattt tattttgcaa ttccttcaat aactgttcga
    55081 taccttgctt taaacttttc tcttctaaat tgactggacg tagatgaaga agcagcgcac
    55141 gcatctcaga ctgggaagcg ttgatgatct ctgctaccat tttcaactgc ttgcgaaaca
    55201 tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc cgacatcatc atcattgctg
    55261 caaacaattg ttgcgaaact gaatcatgca gctcacgggc taatcgatgt ctttctaatt
    55321 ctaaaatttc ttctttggtt tgtccatcta catattgggg acggctcgtt acaatttgta
    55381 attcactaga tacttcaatc attttttctt ttatcttagt gatttcctta tcaatatctt
    55441 tgatgtataa atcgtccgat gcacttggga tcggaagaag caacaatttg gattcataat
    55501 tgccagaaga aagtgcacgc agcttttctt cgattttccc gtattttgtc ttttgaataa
    55561 gtgataataa tagaaagaca attaatccta cacctaagga aatggctaac aaatggaaga
    55621 taagcggaac atataataaa cgtgcacgca gtaattctcc ccaccaatga ctttgattgc
    55681 tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca atccccgaat
    55741 aaacagctag catcgctctg gatatttttc ccatcatatt cgtatcacct cgacatctcc
    55801 aatcaaggta ttagtaataa tcttcaatct acgagggttt tcatcataat cactgctgta
    55861 gattttgatt tgttcatttt tcaaagaaaa ttgctcttct tcaaacaaga catttcctac
    55921 taatgtcgca tgctctaatc ggatggctac gcccaaaggt actaagatcc gtgttctgcc
    55981 tatccctttt cttacaatca caatattatc atcttttggc aaaagcgtat tgcccaaatc
    56041 tatgatagta tcaccagaaa tgacggcgat gttgatatcg tcccattcat agacgtcgtt
    56101 tccaatacgt tggttaccga ataattgctg tcttttccgc tcattattat gtgtttttaa
    56161 ttgctctgtt tggatcatca tgatctgttt ctttcgccaa aaagcatttt tggttaaatc
    56221 tatccctgaa atctctacac ctttcagacc aataaataaa acggcaaata cagccatcat
    56281 ccataaagca ggactgttga ctaagctaaa aaaaatgacc aaacttccta atatcaattg
    56341 aaaattttga aatttagtac gaggatgttt gcgcatagct agatatatat tgaaaatacc
    56401 gaatatcact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag caaataacag
    56461 cagtagtgct tcaactacca caaaaaaacg ccaagaacta ttcatcgctc tttctccctc
    56521 ctgacacatt ctcatttcat catacctatt tttttaactg ttggctatag gactttagat
    56581 gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa aagatctaga gtgtatcatc
    56641 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
    56701 aactaacata tcgccacctg gagtagcaat agttacttca tcatttttac gtttgccaat
    56761 caatgcttgc gcaataggag aatcattgga aattttgcca gaaaacggat ctgcttcagc
    56821 gcttccgaca atcgtgtact cttcttcttc tccatctggt agttcgatga atgtaactgt
    56881 tttaccaatt gatacctcat ctgcatctac accgtcgtta tcgatgattt gagcaaaacg
    56941 aatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttcgctga
    57001 ttcatattca gagttttcgg aaagatctcc gaaaccgcgg gcaattttga ttcgttcaat
    57061 gatttctccg cgtttgactg tttttagttc ttctaattct ttttctaatt tttctttacc
    57121 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
    57181 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttcttc
    57241 aaatgtaaat gtttttctaa aaatgatcaa tgtctagagc ctgtttttct aagaattatt
    57301 tacatatttt tccactaatt ccatatgttc ttcataagtt ttagcgaaat aaacatttcc
    57361 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
    57421 gattgcctct tcgcttggat tgtcaaaagg tccaggtcca tagccggtat tggtatacaa
    57481 attatatggt gaatctacag ctgtatcttc atacgtcact aattctttgt gttcacctaa
    57541 tgcgtataaa acagagatat ctgactgaag tggcatattt gctttgattc ggttaaagaa
    57601 gacttgtgca atgtttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
    57661 tagagttaaa acttcttgta cagtcaaatt tttctgcttg atagctgaat aatagttttg
    57721 catgaccgta ttcgtcttgt tcaccatttc aatcactaga tccttaagcg aagtactcgt
    57781 gtaataatca tatgtggctg ggaataagta accttccaaa cggtattttg tgttctcagc
    57841 tttagatgca ctttccaaca gttctgggaa tttttggtgc aactcgttaa agaaagtctc
    57901 atcattcact aaatctaaaa attctttctt gtctttacca gttaccttcg ctacccgttc
    57961 agcaatctga tcgatatcgt acccttctgg aatggcaatt ttcccatctg caacttttgt
    58021 tggctcagaa gtaccgcctt cttgcaactg tttgccaatc tcgtcaagag tcatattagg
    58081 cgctaactga taatagccag cttgaaagcc agttaaattt ttaaattttg tataataatt
    58141 gaaaacaatc ccgcttttaa taatattgtc cttctccaaa atctcaccaa tctgcttgtt
    58201 agaagagcca ctcgggatct cgacttggac taattggtca tctgatttat ctaaaggctt
    58261 caacccgcta tctacataac gatacacggt aaatccaaga aatccgccaa taatcaataa
    58321 tgtaagagca atcactaaaa tgatcttcct cacaatctta tcttcttttt tacgcagatt
    58381 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tatctttact
    58441 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcaagttc gtaactttat
    58501 tcattataca tgatatttga attggcgtga atgattttat ttaaatattc tatcaaaaat
    58561 gtaagtagta aatccattac tataagatta aaataaatgt tatcaagata caagctattt
    58621 tagctttatt ctttctgtaa aaaagaaagg agactaaata aactattatg aaaaaaggaa
    58681 aacatgctaa aaaaagaaaa tattcatttt taatgatcgt tgtcgtgttg tttagcctta
    58741 gtgcgttctc tacaggaact attcttggat ggcaatttta caataatgaa cactctcaga
    58801 tagttgccca tgctcaatca gctcaaaaaa agttgatact gactaccaat gaacaacagc
    58861 tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta tattggctca atttacgtaa
    58921 agaaaaataa tcgcatcatc atggaaaaag gctatggcta tgcgaataaa ctcacgcaac
    58981 aaccaaatga tccttctctt tattatcaga ttggttcggt tcaaaaagca ttgactactc
    59041 ttttgatttt aaaacaagtc gagcgtgggc acctttcttt agatactaag ctggctcaat
    59101 tctatccaca aattgctggc agccagcaaa ttactatccg tgacttgctg tatatgcgct
    59161 caggcttgca ccgaactgct tctccaacca taccgatgac tgatgaagaa gtcgttcaat
    59221 ttgcgattca gcacttaaaa tttacagatt atcagacctc tcattatgat tcattgaact
    59281 ttaccatatt gactggaatt ttgatcaaat taacaaatca accgtatgaa aaattattga
    59341 ccgatgatat catcgaacct ttaggattac agcatacaaa attttatgac caagtcaaaa
    59401 attcatccaa tcatgcacta tcctatcaaa tgtctaaaga ggatgattac tttaaaccac
    59461 tgacagaatc tgaaacagag atccataacg aattagggac tggtaatatc agtatgtccg
    59521 tctatgattt aaatacgctg tttacacaag ttttatctgg aaaactagtt tccaaagact
    59581 tactcttctc attatggcgt gaaaatgcga atggacatcc atatagtggt ggggtatata
    59641 gcggagataa ttatatcctg tcgcaaggaa atatcgactc ttttgataca tctgctatat
    59701 ttagcaaaga ttcacaggat gctatcatca tggaaagcaa tataaaagct gacaaaacta
    59761 tcaaactgcc tactgctgat ctaagaaacc aaatctatga cctgatggaa ggaactacta
    59821 agttggcaaa tagctgatat gatgaaaaga tagcaaatac tacttctttg aatagcattt
    59881 gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg ggacaaatct aaaaatcaat
    59941 ttttgctaca gactttaatt ccaaataaac agaacagaaa gaggtgttct tctttctgtt
    60001 ctgtccaaat tattctaaga tttctcttta ccaacattat ttgacaaagg gcctattaaa
    60061 tctaaaatag tatctttaga ttgctaaaat atgtgctagc aaatcattca tggcttgttc
    60121 ttatttttaa ttttatgcaa taaaaaagaa gaacaattag cgtgattata gaaataaata
    60181 atgagatttt agaaaaaaat gtgtatgaat acctgattgt tactttattt attccacttt
    60241 cactttttac tgtaacaaaa ggacctcctt gttttagtac tgtagcattt tttgaattaa
    60301 cagtaaccgt taatccttta taccggaata caggtaaaac tattgttttg ccttttgaag
    60361 atttaaacgt aaatgtaatt tcatctttat ttggagaaaa gctactataa ataacttgac
    60421 catcttctgt aacttgatgg tttttcaaaa gcaattgatc ttttttatag cctataggta
    60481 cataatcaaa attctcaaat tcttcaagtt tattttccat ttgttcatta gaataagttc
    60541 cttgaaaatt catttctttc accatgttaa ctactgatga ataat
//
LOCUS       DAZUKT010000006        59637 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000006 DAZUKT010000000
VERSION     DAZUKT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.006"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG816_000374"
     CDS             complement(451..1761)
                     /locus_tag="ACG816_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4919293.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACG816_000375"
     CDS             complement(1773..3191)
                     /locus_tag="ACG816_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4919294.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACG816_000376"
     CDS             complement(3431..4999)
                     /locus_tag="ACG816_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4919295.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACG816_000377"
     CDS             complement(5003..5413)
                     /locus_tag="ACG816_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4919296.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACG816_000378"
     CDS             complement(5410..6333)
                     /locus_tag="ACG816_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4919297.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACG816_000379"
     CDS             complement(6330..7208)
                     /locus_tag="ACG816_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4919298.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACG816_000380"
     CDS             complement(7195..8532)
                     /locus_tag="ACG816_000380"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4919299.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACG816_000381"
     CDS             complement(8535..9638)
                     /locus_tag="ACG816_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4919300.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACG816_000382"
     CDS             complement(9712..11220)
                     /locus_tag="ACG816_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4919301.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACG816_000383"
     CDS             complement(11186..11878)
                     /locus_tag="ACG816_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4919302.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACG816_000384"
     CDS             complement(11900..12874)
                     /locus_tag="ACG816_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919303.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACG816_000385"
     CDS             13174..14046
                     /locus_tag="ACG816_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4919304.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ACG816_000386"
     CDS             complement(14504..16702)
                     /locus_tag="ACG816_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919305.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ACG816_000387"
     CDS             16934..17926
                     /locus_tag="ACG816_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4919306.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ACG816_000388"
     CDS             17986..19407
                     /locus_tag="ACG816_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4919307.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ACG816_000389"
     CDS             19400..20899
                     /locus_tag="ACG816_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4919308.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ACG816_000390"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ACG816_000390"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4919309.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ACG816_000391"
     CDS             22491..23174
                     /locus_tag="ACG816_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4919310.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ACG816_000392"
     CDS             23176..24012
                     /locus_tag="ACG816_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4919311.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ACG816_000393"
     CDS             24023..25777
                     /locus_tag="ACG816_000393"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919312.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ACG816_000394"
     CDS             complement(26053..26394)
                     /locus_tag="ACG816_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4919313.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ACG816_000395"
     CDS             complement(26462..28633)
                     /locus_tag="ACG816_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4919314.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="ACG816_000396"
     CDS             28844..29701
                     /locus_tag="ACG816_000396"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4919315.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ACG816_000397"
     CDS             complement(29772..30629)
                     /locus_tag="ACG816_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4919316.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ACG816_000398"
     CDS             complement(30614..33316)
                     /locus_tag="ACG816_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4919317.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ACG816_000399"
     CDS             complement(33329..34618)
                     /locus_tag="ACG816_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4919318.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ACG816_000400"
     CDS             34768..35814
                     /locus_tag="ACG816_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4919319.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ACG816_000401"
     CDS             35816..37969
                     /locus_tag="ACG816_000401"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4919320.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="ACG816_000402"
     CDS             complement(38442..39893)
                     /locus_tag="ACG816_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4919321.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="ACG816_000403"
     CDS             complement(39890..41614)
                     /locus_tag="ACG816_000403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4919322.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="ACG816_000404"
     CDS             complement(41607..42221)
                     /locus_tag="ACG816_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4919323.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="ACG816_000405"
     CDS             complement(42566..44023)
                     /locus_tag="ACG816_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4919324.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="ACG816_000406"
     CDS             complement(44064..44984)
                     /locus_tag="ACG816_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4919325.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="ACG816_000407"
     CDS             complement(44996..45943)
                     /locus_tag="ACG816_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919326.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="ACG816_000408"
     CDS             complement(46422..47141)
                     /locus_tag="ACG816_000408"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4919327.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="ACG816_000409"
     CDS             complement(47190..48836)
                     /locus_tag="ACG816_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS4919328.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="ACG816_000410"
     CDS             49015..49605
                     /locus_tag="ACG816_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4919329.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="ACG816_000411"
     CDS             49697..51202
                     /locus_tag="ACG816_000411"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4919330.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ACG816_000412"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ACG816_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4919331.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="ACG816_000413"
     CDS             complement(52638..53156)
                     /locus_tag="ACG816_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919332.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="ACG816_000414"
     CDS             complement(53172..53498)
                     /locus_tag="ACG816_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4919333.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="ACG816_000415"
     CDS             complement(53511..55490)
                     /locus_tag="ACG816_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4919334.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="ACG816_000416"
     CDS             complement(55511..55825)
                     /locus_tag="ACG816_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4919335.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="ACG816_000417"
     CDS             55993..56286
                     /locus_tag="ACG816_000417"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4919336.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="ACG816_000418"
     CDS             complement(56364..57476)
                     /locus_tag="ACG816_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4919337.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57629..58477
                     /locus_tag="ACG816_000419"
                     /pseudo
     CDS             57629..58477
                     /locus_tag="ACG816_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(58830..>59637)
                     /locus_tag="ACG816_000420"
     CDS             complement(58830..>59637)
                     /locus_tag="ACG816_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4919338.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17826 a        12189 c        10425 g        19197 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    59341 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    59401 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    59461 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    59521 atctttgtca acacgacacc gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc
    59581 gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg catctaattc atacccc
//
LOCUS       DAZUKT010000007        55823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000007 DAZUKT010000000
VERSION     DAZUKT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.007"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..399)
                     /locus_tag="ACG816_000421"
     CDS             complement(61..399)
                     /locus_tag="ACG816_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS4919339.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(415..618)
                     /locus_tag="ACG816_000422"
     CDS             complement(415..618)
                     /locus_tag="ACG816_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919340.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(633..1409)
                     /locus_tag="ACG816_000423"
     CDS             complement(633..1409)
                     /locus_tag="ACG816_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4919341.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1708..2028
                     /locus_tag="ACG816_000424"
     CDS             1708..2028
                     /locus_tag="ACG816_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4919342.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2046..2477
                     /locus_tag="ACG816_000425"
     CDS             2046..2477
                     /locus_tag="ACG816_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4919343.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            2607..3068
                     /locus_tag="ACG816_000426"
     CDS             2607..3068
                     /locus_tag="ACG816_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS4919344.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            3102..3737
                     /locus_tag="ACG816_000427"
     CDS             3102..3737
                     /locus_tag="ACG816_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS4919345.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            3850..4485
                     /locus_tag="ACG816_000428"
     CDS             3850..4485
                     /locus_tag="ACG816_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919346.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(4478..4612)
                     /locus_tag="ACG816_000429"
     CDS             complement(4478..4612)
                     /locus_tag="ACG816_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919347.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            4784..5923
                     /locus_tag="ACG816_000430"
     CDS             4784..5923
                     /locus_tag="ACG816_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4919348.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(5992..6065)
                     /locus_tag="ACG816_000431"
     tRNA            complement(5992..6065)
                     /locus_tag="ACG816_000431"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6029..6031),aa:Arg,seq:cct)
     gene            6219..6383
                     /locus_tag="ACG816_000432"
     CDS             6219..6383
                     /locus_tag="ACG816_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919349.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            complement(6454..7634)
                     /gene="eis"
                     /locus_tag="ACG816_000433"
                     /pseudo
     CDS             complement(6454..7634)
                     /gene="eis"
                     /locus_tag="ACG816_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced intracellular survival protein Eis"
     gene            complement(7757..8170)
                     /locus_tag="ACG816_000434"
     CDS             complement(7757..8170)
                     /locus_tag="ACG816_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4919350.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            complement(8170..8352)
                     /locus_tag="ACG816_000435"
     CDS             complement(8170..8352)
                     /locus_tag="ACG816_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4919351.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            complement(8383..8604)
                     /locus_tag="ACG816_000436"
     CDS             complement(8383..8604)
                     /locus_tag="ACG816_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919352.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            complement(8672..10132)
                     /locus_tag="ACG816_000437"
     CDS             complement(8672..10132)
                     /locus_tag="ACG816_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4919353.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            complement(10240..10560)
                     /locus_tag="ACG816_000438"
     CDS             complement(10240..10560)
                     /locus_tag="ACG816_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4919354.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            complement(10590..10907)
                     /locus_tag="ACG816_000439"
     CDS             complement(10590..10907)
                     /locus_tag="ACG816_000439"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4919355.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            complement(11059..13761)
                     /locus_tag="ACG816_000440"
     CDS             complement(11059..13761)
                     /locus_tag="ACG816_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4919356.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            complement(13824..15101)
                     /locus_tag="ACG816_000441"
     CDS             complement(13824..15101)
                     /locus_tag="ACG816_000441"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4919357.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            complement(15101..16114)
                     /locus_tag="ACG816_000442"
     CDS             complement(15101..16114)
                     /locus_tag="ACG816_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4919358.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            complement(16130..16858)
                     /gene="sfsA"
                     /locus_tag="ACG816_000443"
     CDS             complement(16130..16858)
                     /gene="sfsA"
                     /locus_tag="ACG816_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4919359.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            17035..17364
                     /locus_tag="ACG816_000444"
     CDS             17035..17364
                     /locus_tag="ACG816_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4919360.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            17548..18195
                     /locus_tag="ACG816_000445"
     CDS             17548..18195
                     /locus_tag="ACG816_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4919361.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            18210..19736
                     /gene="zwf"
                     /locus_tag="ACG816_000446"
     CDS             18210..19736
                     /gene="zwf"
                     /locus_tag="ACG816_000446"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4919362.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19785..22583)
                     /gene="ileS"
                     /locus_tag="ACG816_000447"
     CDS             complement(19785..22583)
                     /gene="ileS"
                     /locus_tag="ACG816_000447"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4919363.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(22845..23552)
                     /locus_tag="ACG816_000448"
     CDS             complement(22845..23552)
                     /locus_tag="ACG816_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4919364.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(23573..24355)
                     /locus_tag="ACG816_000449"
     CDS             complement(23573..24355)
                     /locus_tag="ACG816_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4919365.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(24404..24625)
                     /locus_tag="ACG816_000450"
     CDS             complement(24404..24625)
                     /locus_tag="ACG816_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4919366.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(24706..25305)
                     /locus_tag="ACG816_000451"
     CDS             complement(24706..25305)
                     /locus_tag="ACG816_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4919367.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(25319..25996)
                     /locus_tag="ACG816_000452"
     CDS             complement(25319..25996)
                     /locus_tag="ACG816_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4919368.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(26013..27254)
                     /gene="ftsZ"
                     /locus_tag="ACG816_000453"
     CDS             complement(26013..27254)
                     /gene="ftsZ"
                     /locus_tag="ACG816_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4919369.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(27277..28602)
                     /gene="ftsA"
                     /locus_tag="ACG816_000454"
     CDS             complement(27277..28602)
                     /gene="ftsA"
                     /locus_tag="ACG816_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4919370.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(28758..29978)
                     /locus_tag="ACG816_000455"
     CDS             complement(28758..29978)
                     /locus_tag="ACG816_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4919371.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(29993..31081)
                     /gene="murG"
                     /locus_tag="ACG816_000456"
     CDS             complement(29993..31081)
                     /gene="murG"
                     /locus_tag="ACG816_000456"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4919372.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(31107..32468)
                     /gene="murD"
                     /locus_tag="ACG816_000457"
     CDS             complement(31107..32468)
                     /gene="murD"
                     /locus_tag="ACG816_000457"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4919373.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(32482..33444)
                     /gene="mraY"
                     /locus_tag="ACG816_000458"
     CDS             complement(32482..33444)
                     /gene="mraY"
                     /locus_tag="ACG816_000458"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4919374.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(33470..35662)
                     /locus_tag="ACG816_000459"
     CDS             complement(33470..35662)
                     /locus_tag="ACG816_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4919375.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(35663..36064)
                     /gene="ftsL"
                     /locus_tag="ACG816_000460"
     CDS             complement(35663..36064)
                     /gene="ftsL"
                     /locus_tag="ACG816_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4919376.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(36069..37028)
                     /gene="rsmH"
                     /locus_tag="ACG816_000461"
     CDS             complement(36069..37028)
                     /gene="rsmH"
                     /locus_tag="ACG816_000461"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4919377.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYYVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(37049..37480)
                     /gene="mraZ"
                     /locus_tag="ACG816_000462"
     CDS             complement(37049..37480)
                     /gene="mraZ"
                     /locus_tag="ACG816_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4919378.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(37650..38018)
                     /locus_tag="ACG816_000463"
     CDS             complement(37650..38018)
                     /locus_tag="ACG816_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4919379.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            38189..39145
                     /locus_tag="ACG816_000464"
     CDS             38189..39145
                     /locus_tag="ACG816_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4919380.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            39218..39334
                     /locus_tag="ACG816_000465"
     CDS             39218..39334
                     /locus_tag="ACG816_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4919381.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(39389..41071)
                     /gene="recN"
                     /locus_tag="ACG816_000466"
     CDS             complement(39389..41071)
                     /gene="recN"
                     /locus_tag="ACG816_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4919382.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(41089..41538)
                     /locus_tag="ACG816_000467"
     CDS             complement(41089..41538)
                     /locus_tag="ACG816_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4919383.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(41645..42463)
                     /locus_tag="ACG816_000468"
     CDS             complement(41645..42463)
                     /locus_tag="ACG816_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4919384.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(42472..43362)
                     /locus_tag="ACG816_000469"
     CDS             complement(42472..43362)
                     /locus_tag="ACG816_000469"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4919385.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(43364..43588)
                     /locus_tag="ACG816_000470"
     CDS             complement(43364..43588)
                     /locus_tag="ACG816_000470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4919386.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(43593..44930)
                     /gene="xseA"
                     /locus_tag="ACG816_000471"
     CDS             complement(43593..44930)
                     /gene="xseA"
                     /locus_tag="ACG816_000471"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4919387.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(44931..45785)
                     /locus_tag="ACG816_000472"
     CDS             complement(44931..45785)
                     /locus_tag="ACG816_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4919388.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(45882..46331)
                     /gene="nusB"
                     /locus_tag="ACG816_000473"
     CDS             complement(45882..46331)
                     /gene="nusB"
                     /locus_tag="ACG816_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4919389.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(46324..46749)
                     /locus_tag="ACG816_000474"
     CDS             complement(46324..46749)
                     /locus_tag="ACG816_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4919390.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(46995..48059)
                     /locus_tag="ACG816_000475"
     CDS             complement(46995..48059)
                     /locus_tag="ACG816_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4919391.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(48231..48476)
                     /locus_tag="ACG816_000476"
     CDS             complement(48231..48476)
                     /locus_tag="ACG816_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4919392.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(48615..48989)
                     /locus_tag="ACG816_000477"
     CDS             complement(48615..48989)
                     /locus_tag="ACG816_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4919393.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(49072..49986)
                     /locus_tag="ACG816_000478"
     CDS             complement(49072..49986)
                     /locus_tag="ACG816_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4919394.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(50007..50810)
                     /locus_tag="ACG816_000479"
     CDS             complement(50007..50810)
                     /locus_tag="ACG816_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4919395.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(50843..51832)
                     /locus_tag="ACG816_000480"
     CDS             complement(50843..51832)
                     /locus_tag="ACG816_000480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4919396.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(51935..52426)
                     /locus_tag="ACG816_000481"
     CDS             complement(51935..52426)
                     /locus_tag="ACG816_000481"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4919397.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(52617..55424)
                     /locus_tag="ACG816_000482"
     CDS             complement(52617..55424)
                     /locus_tag="ACG816_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4919398.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
BASE COUNT        16216 a        11699 c         9403 g        18505 t
ORIGIN      
        1 ctgcttcccc aaagtcctaa agcgtagatg attagcggtg cgctgaacac tagcgctcta
       61 ttaaattttc ccatccgctt gcctccttaa gatttccgaa aaatttgttt ctaaaaattc
      121 aagtgtttta cttcttaaaa atagatatgt gtctcttcct tcaactggat aatagacaaa
      181 tccatttttg tttttctcga tatcgataat atttctatat cttgggttct ttaaaactct
      241 agaagtaaac caatcatatt ttctgttaat ccgttctagc acttctggca acgtcatcca
      301 tctaccagta tcatcagctt tttttaactc ctcataatcc acttgggaga taattacata
      361 gccttctgga attgggattt ttgcttctag atattgcatt agctgttcct ccctttacaa
      421 ttcgtacata gtgataatcg aatctataat tctgtttgct tctgcagaag tctttttacc
      481 gtttaaaatt aaagataagt agcttttgct aattccaaat ctttcagcaa gcatggtgta
      541 agttaagaac tttgaacttt cgacatattc tttgattttt tctctatctc gttgagtgat
      601 ttctgcaatg tcagtcatac taaaactcct ttctaaccaa tttcctctaa atccatttga
      661 gggtaatatc cttctttttt tagtaattcg tagataaata gacgcccttt ctgtgtccat
      721 ttggtattca ttacaatttt agtgccacca tcggctttcg ggatctcagt tgtatgagat
      781 tttgtgtatc cttggttcat gtgttttttg cacaataacc attggttacc gacttttttc
      841 tgaataccta gtttatgaag taatttattc atctgttgtg gagacatccc ataatctgct
      901 gcaatctgac taattgttac tgaatctgta gaagataata tgctatctaa ataggagatt
      961 ttaggttcgt actctgcaat cttttgttct gcgattaatc ttccagttct ttcttctttt
     1021 agttgagttg ctaattgaat gattgtatct ggattaagca aagcttcttc tactttttct
     1081 ggagttagat aacctccatg ttttctaatt gctggcaaca cttcacttgt tacccaacgt
     1141 ttaaattttt tggcagaggg aagttttgat tttaagatta aactgtacaa accagattcg
     1201 ttgatgattg tcatctctct tgattgacct gaggtcgtga ttcgcgaccc catcttatct
     1261 tccaaatcta catgccgcga caaagcatct ttagtgtttg aataacccaa aacactcgca
     1321 acgtctttgc ctacaaaata tggttcatca tttactaaaa ttgtccgaac ttcgttttgt
     1381 tcgaaattaa aaatttgcgg tgtgttcata tttctcattc ctttctttta tattcgtaaa
     1441 caaatttaac aacttttttc taaattcaat tgacagtatc tgagtaatac tctatactaa
     1501 gagcataatt aaataagaca taaaaacatt gattttaaag ctttcttggc ggtctgcatt
     1561 tacatatcaa tagtgttttt tgttgtcttt ttagttgtta aatctgttta caagatatag
     1621 tatagctcat tactcagaat tagtcaaatt atttctgcgt atttttctaa actttttttg
     1681 taaacattta gaaaggttgt tttatcgatg aatacctatg aaataataaa agacttggcg
     1741 aagaggaaaa agatatctat acggcagtta gaaattaatt ttggatattc taacggttat
     1801 ctagcaaagt ggaaaaataa tactcctaat tcagatgaat taagtcggat tgctgactat
     1861 ttcgatgtct ctgtagacta ccttctaggt agagaagaaa gagaaacccc taaacatgtg
     1921 gatttatcag aagacgatac tgttttttct tttgatggaa aagaaatatc taaggagaca
     1981 atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg
     2041 tttgtatgta tttgaaattg aaagaaatgt tgagcgaata caatttgaaa gtagtttata
     2101 tggagatgaa agagcctggt ttctactatc caaaacccag aatcattttt ttgaatgaaa
     2161 atctatacgg tgaaactgca gaagcctttc atttatctca tgaacttgca catttcagcg
     2221 cctctcattt tgaattttca gttttgtacg atacttctac aacttttcac tcaaaatttg
     2281 aaacagaagc agataaaata gctattctaa ttttattaaa tatctatatt gagaacgaac
     2341 tgactgatga atctcagttt aatctcgaaa aatttatgga gtattattct attcagaata
     2401 agctcagata cacttgttat gctgtctgcc aatgctattt taagaaaaaa tattcttacg
     2461 caaggcaata tgtatgaata catcaagaat tattaaaaaa accgatttca gcaaaacatt
     2521 aaaaaagccc gtgctgcaac acgggctctt tcctcatata tgagcttcta caaaaaaaat
     2581 catatcacag aaatgaggaa gaaaaaatgg aaaaagagat tttaataaaa tctccaggaa
     2641 aaaccttgat taaagtaact gatgattcta tttcaattat tagaaaagga tttataaatt
     2701 tagttaatca aggtattaaa ggagaaaaaa ctatcccctt taaaaatata tcagccgtac
     2761 aattaaaaaa accaggtatg agtaatggtt atatacaatt taccttgcta ggcggaaacg
     2821 aaagtcgcgg aggtatatta gcggccacga aagatgaaaa caccgttatg tttacaaaaa
     2881 aatattggaa tgaaatggaa aatttaaaaa aatatataga gaagcaacaa tctatattag
     2941 ataataattc caaagaaaca caaatttcaa gtgcagatga aattaaaaag tataaagagt
     3001 tgttagatga agggattatt aatcaagaag aattcgattt taagaaaaaa gaattgcttg
     3061 gtttataata ttcaatatca aaagagaaag aggatctatt aatggacggt atatttggtt
     3121 ttactggtat tgttttattt attataggtt taatcatgtt aattgtaaga tttatcaaaa
     3181 aaacaaataa gaaaacacct atgattctcc ttattatcgg gattattttt actaccgtag
     3241 gtttctcgct ttcctcatca aacgaggcaa atgacaataa caaacaggaa agctcatcat
     3301 ctgatactca aactactaca tctaacagca gcaataaaag cctaactcaa tttgaagaat
     3361 ttttagagac aaataatcat gactggggtg ctttcttaga tagttattat tctattactc
     3421 cagctactga gcaaaataca gcttttacta aatatattag tggtaaaaca tatacttttg
     3481 aaggaactgt tatcgaatca atgactacta gaatagccat tatagcagac aaagagtacg
     3541 atgataaaag ttggaatgat atttctgcaa cacctaaagt ttcatatgta atttttgcga
     3601 aggacttaaa caatgctgat acctttacaa agggcgataa agtaactttt acaggagaaa
     3661 ttagttcaag aggatcgaac atagaaaaat cctatgctca atgggatatg attaatagta
     3721 aagtaagtaa aaaataaatg aaccacgccc cactaagagt tagattgtgc acaataataa
     3781 aacctataca aaagagtgcc atcaggagtc acaatgcttg taaaggaaat gacaaaatag
     3841 gaggtaacta tggataatag caatgaaaaa ctaaccaaaa aagaatatct tgaattaata
     3901 gttgaaggcg gtattggagc aattccttat attggtggcg ttgtgcagtc actttatttt
     3961 gggtctaaaa acgagaagcg tttcaaacgc atcgagaaat tctacaaaaa tttaaacgaa
     4021 tcattagaaa acattcgcga tcaaattcca gaagatgttt tcaacttaga gaacaaggac
     4081 caactcattg gaatattcga agggataaat gacgaggtag aaaaatcaaa ggcacaaaat
     4141 aaaatagata tgtataaaaa tctttataag aattgtttac ttcgaattaa caacgcttca
     4201 tgggataacg aggagtatta tcttcttgta ctgaatcaat taacatcgat tgagattcaa
     4261 ttgttagcat gcttaatgag tcgtggaaac gaaaatttca ctggaaatat cagcatgtct
     4321 ggctattcac aagagctaat agatggtagc ttaaatagat tatctgattt tggtctgtta
     4381 gaaaaaacga ttagctccat agtattaggt gatgtcggca aacaaaatat gggctacaaa
     4441 atatctcatt taggaattca atttattaac tcaactctta cttaagtttt ctttctaata
     4501 tgacatctag acctatcagc aatgcaacaa taaaaaacca gacataccaa taatcaacaa
     4561 ataatctaaa ctctggaagt tcagttattc ttttaaaaat cactttaatc ataataaaca
     4621 cctctgattg gatgatctaa tacacatcca attataccat taaaacgaca aatagcacat
     4681 ccttgcgccg gcaagcaaaa aggatgtgca aaaaataata aaacccaaac aatgggcttc
     4741 tttatagttc ctattgtatc agagaaagag agctgattca attatggcaa gattagtcaa
     4801 acgtggaaat agttggcaat acgaaatttc atacaaaaaa gatgacggaa aatacacgaa
     4861 gataagaaaa tcaggattta agacaaaagg agaagcaaaa gatgccgcca acgaattaga
     4921 atataacttg aacaaaggcc ttaaagggga tcgcaaaaat ctattattat cagattactt
     4981 tgaggattgg atgcaacttt ataaagaagg aacagtatct cctatcactt atagaaaata
     5041 cgaagatacg ttaatgaaca taaagaaata tatgccagcg gtattgattt ctgatttaga
     5101 tagagtggga tatcaacgct ttttaaataa gtatgctaaa gaccatgtaa aatctaccgt
     5161 tattaagttt aataaccata ttagagcgtc gttgaaagat gccgtagaag aaggattaat
     5221 tccgtttgat ccaactagaa aagcagtaat caaaggaaaa gattcattga agccaaaaga
     5281 agataaatat ttagattatg atcaatttaa atctttaatg aaactcgtag aagaaaacct
     5341 ttctgcgcag tactcttctc ctatgctcgt gttagttgct ggtgccactg gaatgcgatt
     5401 tgctgaactt ctaggattaa catgggaaga tatcgatttc gaagatcaaa tcatcactat
     5461 taataaaaca tggaattata aattaaatga atggggaaaa acaaaaaacg aaacttcaaa
     5521 taggaaaatt tccattgata aacatacgat tgatctctta aaaaagttta aaatcaatca
     5581 aaaagaatta ttcgagaatt ttgaaatcaa aaaccctcat aattttgttt ttttcaactt
     5641 aaaaaatgga ttagtttcat caaacgccgt cagcaaatat ttgcgcaaaa aattaaaaga
     5701 attagggatt gaaaagcaat ttactttgca tggactaagg catacacatg catctatttt
     5761 actttatcaa ggagtaaata tacttagcgt atcgaaacgt ttaggacata gcagtttaga
     5821 aactacaatg tctacttatc ttcatattgt tcgagagctt gaagatcagg ataaagaaaa
     5881 aatcaatact gtgttcgata gtttatataa aaatgataac tagtttggca catttttggc
     5941 acaaatacaa aacaaacccc tgtaccgcaa aggatacagg ggttatttat acgttcctga
     6001 ctggaattga accagcgacc tatcgcttag gaggcgattg ctctatccta ctgagctaca
     6061 ggaacaattt caataaatca attgtagctt gtttgttttt tacttgcaag cttatctgct
     6121 aaaaaaatca gcttataagt taggcaaaac gtttccacta gtccttaaca gatgataaac
     6181 tagaggtaga atgaaataat gtagggaggt cctttccaat gaatgataag ccaaaagaaa
     6241 aagtatacgc agaacgctta gattcatcta aaaaaaatcg ggaaaagatt acgaaagaag
     6301 caatccaaga ccacaaaaaa gaaaacccta aaacaaaaga aaacaaaaaa ttcaaattta
     6361 cgaaaaaaga ggatttactt taacattttc cttcaaatgc caaatgaaaa acaaaagggt
     6421 ggtgacaaaa atattgtcat cacccgtttt ttatcaaaaa tagtcattaa gaattggctg
     6481 ttgctgtggc aagattgagc ttaacgtttc ttcatattct tttgtcacct gtattctttc
     6541 ttggaatacc aactcttcca ctttacgata ccctaataat agctgcgtga tatgttggat
     6601 acttcctcga acgattggga gttttgtttg ttctactttt gagagatgat tttttccatt
     6661 ttcgattgtt acttccacga ttccttcatt ccatttcgca taatgatctt cttctatttg
     6721 tagggcaaat gcagcttgtc tctttttaaa tggataattt tccaaaaaca aagaaatatc
     6781 tacgatcctc gccatcatat aaggtgtgat cattacagaa gcaaatggat tttctaatag
     6841 ataattttga tcattaccgc cgtagccgca tgaatattca aatgtttcaa aagcaccatt
     6901 gtgagaactt acgaagcgaa caaggctgca aaaagcatca tgatccaaaa atccccactc
     6961 cgcaataata aaggaagacg ttgcaagctg ataaacgata tatcccgttg cttcctcatt
     7021 atcatcaaaa tgcaaagcaa actggtaatt gcctttttgc ttgaatttat acgtatacca
     7081 ccagtcttcg cgcttcagac cagctcgttg gttttccggc atctgttcat aaattcttct
     7141 gattgcagct tttgcatctt caaaatccat acgctttatt tttccagatt ttttttgata
     7201 aacggcacat cacgactgtt tagttgataa ctgattttat caaatgattg ttcatagcca
     7261 tagcgccgat agaagggata ggagaatggc gccagataag ataagcttac tttacgatca
     7321 cggcaatctt caaggatctt ttccatgatc tgattgatcc cgccttgtcc ccttgcttct
     7381 ggataagaag caacatagcc aatgccggcc attaggtact cttgtccgta aaaattcact
     7441 ggaaaagggg tcgccatgac ttgacttgtt agccgatcct cctcatcaaa aaatccgtaa
     7501 ttccaagaat tctccgcaag tgtacgaaaa cgttctcgcc tttcaggtgt atctgcccca
     7561 ttaaatgcat aagtggctaa atcaaacatt tcctcaatcg aagatacacc tagctctctt
     7621 actttgtttg tcattttgct gccccttcct acaaaaaagc tagtgaggat tcgcaggaaa
     7681 gatctatctt cctatgaaaa attccttcta gcttacttca tttttcaata actaaatagt
     7741 accatgtttc attgatttaa tacgttgctt cgatcatatc tagcattttt ttgaaacgtc
     7801 tccgtttaaa atacatcaag attgttccta aaagcatatc attcaacttt tgaagcatgc
     7861 cataagactc cattttttct gtgtaatgaa cctcacaggt cgaatcgtcc aatggtttga
     7921 tttgataatg aacgacgaat tcattcttcg ttgtcgatgt acgaaactta taagactggt
     7981 tttcaatgac ttcttcgatt tttatgcgag cccggttatt ttgcgagaat tgtttgacgt
     8041 actccaaatt tttcagctgt tttctagtta ctgatttgcc ggtcgcttta cgtacatcga
     8101 aaaggacaga gtccataact ttatcgtaaa aaaacgatgc tggaatattt aattttttcg
     8161 tgatttccat tagttatttt tctcctttgc ttcttctctt tttttcccct tcatgaagaa
     8221 atagattcct aatcctgtta gaaaaagacc tgtgaccata ctatacaaat catacctttg
     8281 cgaatgtgga ttcaaactat acaacacgat caaagaacca atactacata aaaataaacc
     8341 gttttttatc atctcaacca tcctatcttt ttaaaccatt ttctaaacag ctgttgattg
     8401 aaaataaagg actgttcccc aaggctgtgc tttgattagt tcctttgctt ttttagccgc
     8461 aacttccgga tcttctgcac ttgtttcaaa gaacagcttg attccttgtt tttctttaaa
     8521 tgtaaaagtc attccttctt cattgtatcc ttctaacaat tctttgattt catcttgttg
     8581 cattgcactg gcaattgtaa tcatttcagt tcctcctcga aaattcatta tgaggaacga
     8641 aacttaaagc gtcccgctcc tcacgttcat cttattgttg ttcgcctgcg gcagggtcca
     8701 ttttgtttgc agagatgaca aatggtgccc aaatcaagaa agtcacgacc atacatacta
     8761 atgttacgat cggtgcccgc caatccgctc ctgttgctaa gaatgacaat aagaacggcg
     8821 gtactaccca tgtgacttgc tgcgataccg gagcgactag gccaagataa gtagctgtcc
     8881 atccaattgc tgttgctact actggagcaa tcaagaatgg gataaacatg attgcattca
     8941 atacgattgg cagaccaaac atcactggtt cgttgatatt gaagatacca ggcccaatgg
     9001 ataatttagc tactgttaga taatctgcac gtttagagaa aatcaagata gctatcagta
     9061 agacgatcgt tccgcctgat ccgccaaacc atgcaaaagc atcgaacgat ccgcggaccc
     9121 acatataagc ttttccatct tcgattgcat ctaacacggc tggtgtacca gtttttccgt
     9181 tataaccttt ttggaaaata tcaatattga ttagttgtgc ttgtccccag attccctcta
     9241 gcactggtgc caatacattt gatccatgaa taccaaagaa ccagaatact tgaacaaata
     9301 ctgtgacgat caatactgct cccaaccctt gagataatcc taggaatggt tctgcaatat
     9361 atttttgcac caaatcaatg atcagttgcc catcagctac acgacctacg atataattga
     9421 taatagcaat gacgtataaa gcgatcgtcg ctggtaaaat ggctgcaaat gctttagata
     9481 ctgctggtgg tactgaatca ggcattttga tcgtaatgtt ggcaagcatc aatttacaaa
     9541 aaataatgac tgaaatagct cccatgatca taactgtaaa ataagcgttt ccattcaaat
     9601 gatctagttt tagccaaccc cataatccaa ctgacagtgt gccatctgca atcgttgcac
     9661 caatctcaga actattgatt gcatctgcga catttgaagg aacattcaca tctaatgctg
     9721 ctgataatgc tggaccaaag gaaatacctt gaatcaaggt agctaaacca acgatccccc
     9781 cagctagatc atttactcca taagctcggg caagattata cccccaagaa aaggcaaaga
     9841 tcaatccggc gattgctagt gtaccgttcc agacaaaacc attgattccg atgatgacat
     9901 tcaatacggc aaagattcct ttgtctgctg ctaaactgtc agcataggta ctgttaaact
     9961 gttgcggtaa atcacgaata attgcattga tcattacagc gactgatccc gccattgttg
    10021 cgggcagtat gccgataaaa gcatcacgca aagcaactaa atgtttttgc gctccgattt
    10081 ttgcggcaac cggcaggatg tatttctcca tccaacttga tagtccgttc attttttact
    10141 tcctcctata agttttttat ataaagagag ttctccttat aacaaaataa aaaatccgtt
    10201 caaaaatttt tcttcttccg ttcaaaaaag aagttgtatt tattcgctgt atacgcgacg
    10261 ataaagttcg atgatttctt ttgctaaatc agtaaacgca atagatgtca ttaagtgatc
    10321 ttgactgtga acagtcaata atgtcacttg catatgattt ccttgtgctt cttgtgtcaa
    10381 catgtctgtt tgtgcatgat gcgcttgtat aagtgattct tctgcatcag cgattttcct
    10441 atccgctaac tcaaaatcac cgattttagc agccgcaatc gcttccattg catcgctctt
    10501 tgcattaccg ccaaacatga ttaaccccat gatggcttct agattttgtt gatcttccac
    10561 gttgttatac ccccaactgc atacttgatt tatttcatta gattttctgc ttgtgcaaga
    10621 actttttctc cgttcatcat gccatagtct tgcatattga tgacatctaa tggaatacct
    10681 ttaggcgcaa gtttttgttc aaattgagct ttcatgaaac gtacttgagg acctaataat
    10741 aatacatcga cattcttaga ttctaaatta ttatctgcat ctgaggcaga gaccgcaaaa
    10801 atatcggctt ccatcccttt ttcttctgct gctttttgca tttttgtgac caataaactc
    10861 gtactcatcc ctgctgaaca aactaacatg attgtttttt ttgccatttt taacactcct
    10921 tttatttttt tacactttta tataacgcaa gaagcatgcc aataaaaaaa agagctgaaa
    10981 tagcgttttc atgcttttta gctctttttt atagtgcgca cacagatgtt tagtgtgtaa
    11041 acagtatgat tagtgtgttc atttttttat tttattttca agaaacattt gtgtaagaaa
    11101 tgcgatatca tcttctggaa tttttacctg atattttcgt tctagtgcca tcaagtttgc
    11161 ccgaacgata tccatttcca aacgataaat ttttgagaaa atagacagtt tctcaaattg
    11221 ccgatggctt tcttgtttga tcaatccgtc taacatgaag gctaaatgga taacgattcc
    11281 agtatctact ccaggttcca cgataatcga caaatcggtc tggatctgct gaactgtctt
    11341 ttgcaatgaa cggatcagtt catcaataga aaagacatcg cttaatgatc cagcaagtga
    11401 atcgacaatt ttagctgtcg ggatctcatc atcagctata cgctttaaaa tatttagttt
    11461 ttcatcatca aaaacgtcat aggctgaaaa aaatgggata ttttgataat caacatcaac
    11521 tgttcccgca attgctttga tctcatattc ttccaataag gcatcgatgt gttttttgaa
    11581 tgattcacgt tctaaaaact gcatctggat cagttctacc ttattttgat taatcactgg
    11641 cataatacgc tgatataatt tggcagctac tccttcacca gtaaagcatg tcacgattac
    11701 ggctttttta cgctgtaaag tttcagactt ttccgctttg aactgatcac gtacgatcga
    11761 ttcaaaggaa agctggatat tttgataaat atcttctaag cttcttccaa cacttgccat
    11821 ccgtatcgct tctatcacaa tcatggtact tgtcatcgtg atagctttcg tccgaatgcc
    11881 agtctcttca taaatcaaat cactgaaggt gtttaatgat cccatatctg ttaacaagag
    11941 taggccattc gtcaaatgct cttgatagtt tttgacatat tgtaacactt cttcatacat
    12001 tgtttgaact tccataacca gtggcatatt aaaagctttc cctacttttg ttcctaatag
    12061 ttcttgtgcc atctccaaca tgcttgaagc agtctgtctg ccgtgcatca atatgagaac
    12121 atctactttg ttttctttaa caggctcact ttctcctaga ttgatagaaa gaaacatact
    12181 aataaagccg atttcatcaa aaggaatctc aacttgttct tcttcttcaa tgatcgttga
    12241 aagatcgatt gctacctgga attccctctt aaaatttttc cgtacgttat tcaaatctgg
    12301 gtggacgatc atatgacctt ctctcacacg atccaatgta ctttgcagat gcaaagcaaa
    12361 agcaaaacga gctttttcat tataaatacg gtctaaccgt ttttcagcca catcatacaa
    12421 ttgattggtt aaccgccaga tttcatctgg caaaagatct ttgggaatcg aacttttagt
    12481 caattctttg acatatgtct ggaaataagc atccacatct tttgaaatga gggtctctag
    12541 atcaatgttc tccacaccgg tttcggaaag agaagtgact ttttcctcaa tatcgttata
    12601 gacctgcatg tttcgttctg gatcttgtga ccagacaaca tcagcaaatc ctggttcaaa
    12661 tgtcaaatac tgacttttgc tgtccatgaa tcggtcgaga cgttccggta tttccttgat
    12721 tttcagtaat cctttttgta cttgaaggga aagttcttct tttcgaatga ttagcttttt
    12781 ctcatgatga gtacggtaat gtaaaaaaga ttttgcacag actagtttca agtctcgttt
    12841 cacttgaccg atatttcctt ccgcatcata aagcatgaag gcaagaatcg cttctttttc
    12901 gatatcgata cgttgattca aacgattcgc ttcttgtttg ataaaaagag agatgatttc
    12961 ataacgctca tccagtgaac gtgaagcaag attcggcaag gtgattgcca taggaatccg
    13021 tcgattgaac gtagataaga aactttctga agattcagtc gtcgccccaa tgatctgaac
    13081 agatgcttca tgtacttgtg cactttctcc aagcggacgg taaatacctt tatcaatgaa
    13141 agtaaacagc atttcctgcc cttccggcgg taaacggtgg atctcatcta aaaacaatat
    13201 accgccgtct gctttggcta ttagaccggg actatcttga gctgctcctg tgtaagcccc
    13261 ttttttgatc ccaaagatgt gtccaaagag cagttgcgga ttttgagcat aatccgcaca
    13321 attgaaagaa acaaatggcg catctgcaga aagcatctct gaatcgattg caaaatgata
    13381 catacattca gcaaacatcg acttccctgt accagtttct ccaaaaataa tcgtatgcaa
    13441 cccccgcggt ggataaagaa tcgcagcttt tgcctgctgt atgctgactt ttaaagaatc
    13501 attttctcca acgagtgttt caaaagtcac ttgcgtagaa gagtcgacat gcacttcatc
    13561 agattttgac ttttccgttg gcaaagagta aacgactggt cggccagcgg tttttacaat
    13621 atgatgggcc ttgtatagtt cacttaaata tcggcttgca ttgcttcgat cgatagttaa
    13681 gcgctcagct acatcacttg ctgtcaatcc ttgttggttt tctgtcaata gctgcaagat
    13741 ctcttcttga cgttctttca cataacccct cctctggcaa ccaattttgt tcccgttttc
    13801 ataatcatag cataaaaagg cgcttatttg cgccttgctc ctttatttct ctcttttttt
    13861 gatttatttt taactttttt tcttattttt tctttgttat attctgggtt ctgctccttt
    13921 ccagaaaaag ttttcgcata ctttttattt actgcaggag ctgcatcttc tttggttggc
    13981 tgctcagttt gcagcttacc tccgtataaa aaaatctctt caagtgttat atttgatgga
    14041 cgtaaaagcc gttttaaatc tcttagatta ttatcttgta cgagtgtaat tactattcct
    14101 tcattaccca ttcgaccagt tcttcccgaa cgatgcaaat aagcttcttc acttaacgga
    14161 acttctgcat tcactacata aggaagacct gtaatatcta aacctctagc cgccacatct
    14221 gtggtcaaaa gtgctctgac cattttcttt ttgaattgtt ctagcgctgc ttttcggagt
    14281 agcttgttct gatcagaggc cagacttcct accggtacac ctctatacag caatttttct
    14341 tccattacgc ctaattcagt cagttgatta aagaaaatca gaccatgaaa atcttctata
    14401 tgcgtgagac gtctcaagta gtcatctttc tttcgtatcg gtacacgtaa aaagtaatgt
    14461 ctcagtcctt ttcgagaatc gtcctctgct gtcacgtcaa ttgtctcaaa cgttttccca
    14521 gtgatttttt caatgctttc aagggaacga tctgctgtcg ctgaaaaaaa agctagctga
    14581 tactctgttg gagcttgatg aagaatttga tcaatgattt ggctgttccc agcgtcaaat
    14641 agctggtctg cttcatcaaa taccattgtt ttcacttgat gtgcttttat tttttttgct
    14701 ttcatcaatt ccaatattct tcctggtgta ccaatcaaga tttctggttt ttccttcaaa
    14761 ccctcaatct gacgtttgac gttggctccg cccaccaaag attggacttt caactctttg
    14821 tcttttcccc attcacgtgc aacatctgct acttgaatag ctagttcttg agaagaagca
    14881 aaaatgacca acgcacttgc ttcacctggt tccacgttta gtagtagagg ccaaagataa
    14941 gccagtgttt tacctgaacc tgtaggagaa accgctacta tactttgttt attacttagt
    15001 ggctgaaaaa ctgctcgttg aataagagaa ggtgagataa accctttatc ctcatatttt
    15061 ttctgccaat tttcgggaaa tacttgtaca aattcgttca ttaaaaatct tcctcattat
    15121 ctgcatcgaa tttgatgccg ccattttttc ttaaatcata aatcacttgg ttcacatttc
    15181 tagctagctc gatccactct tcatacaaca atccccaagc tggatctttc ggatgagcca
    15241 tgacattggc aaaatcctct gcttcatctg ccattggatt ttctgcagct gttaccgcca
    15301 aatcaatgat ttctttatgc gcgcgatcat gaaattgtgc ttgttcaatt gcattgactc
    15361 cattcatcac tagcgttcct gagtcaaaat aaatctctga cggtaaataa gaatcaccga
    15421 ttttacctgg ctggatcgat acatcaaaat tctcataacg taagatcccc catccaagac
    15481 catctacacc agtagaaatc tttcttggga aataatggac ttcttttggc attccaaacc
    15541 aagcaacagc tgcatataca aggtaaacac ctaaatccat caatgcacca cctgagaaat
    15601 gagtcgagaa gatattcggt tcttctccat ctaatacttg gtcatagcgc gaagaatatt
    15661 tcatatagct gaaatttgca ccgattatct cgtcttttaa tggtaagtaa tcggcaatct
    15721 ttttaaaacc tgattcatgg atatttcttg ctgcttcaaa aaaatacaca ttgtgttcat
    15781 ttgccaaatg gatgatttca tccatttctt ttggcgtcga aaaagctggc ttttcaacta
    15841 tcacattttt tcctgccaaa attgcttgct tagcttgact aaagtgaaga ctattcggag
    15901 aagcaatata aatggtgtcc atatgagtaa tattgaaaaa agtcttcaaa tctgttgcga
    15961 attcgatatc cccttcataa tgagaaccaa attcctgtgc tttttccaaa tgtctagaat
    16021 ataccgctgt caattcatat ttttttgtgc ttaaagctgc ttggacaaat tgatgagtaa
    16081 tccaatttgt tccgataata cctaagttga tcattatttt accttctttc tattgtatag
    16141 gatcttcaaa agggatatct acatcaaatg gaatggcctg cttgacagtt acctcatctg
    16201 ccgatacttg acaattatag gcgaggattt gaactccgct ttgctgcgca tgtcgaacca
    16261 tatcagctag tgctggttgc atttcttcat ggatcgttgc cagatgaaga tgttcgaact
    16321 gagcaataaa caagacataa gcggcataac catcttgtgc ggcatacgtt aattctccta
    16381 tatgtttcaa tcctctgagt gtgggcgcat caggaaaagc accaatcgct ttattttcaa
    16441 gtgtcatacc tttgacttca acaaatgctt tttgtccgac caccgtctct aaataaatat
    16501 caaatttgga gtgaagataa gttacttcac gcttgtatac cgtaaatgga ccagttaatc
    16561 ctggtaaaag gatcgtgccg tctaaaatac catcaattgc cagccgatta ggcaattgac
    16621 tatcgatatt aaaccagtca cccttctttt ttacagcaat cagatcatag gcggttttcc
    16681 gtttaggtcc aggagcaaat acaatagcca cttcagcgcc tggtatcagg acctcttttc
    16741 ctctacctgt atttttgaca tgcgcaataa ttatttctcc gtcttttttc atacaatgtg
    16801 ctataaaccg attcgggcgg tcaataaaaa aagctatttc cacttgctga tacttcaaat
    16861 tatgtcacct ttcttcctac tatgtatcct atcataattt gtagaaaaac agtaaaaaaa
    16921 ccagtcgaaa gtcttagaga aattttagct tttattcggt acaatgaaat aaagaagaaa
    16981 gaatatgtct atgcgattat tttctcataa aggagtgatt tggtgataaa aaaaatgatc
    17041 tattttcttg gttcactcat taccgttctc tcaagtttta tttacttgaa aaagaaaaaa
    17101 caagcggaaa agtcagaagg actctcaaag tatcaaggta catggttctt tagtgatagc
    17161 cagcaaaaac aacaattaca gattacatct gattacacgt ttttgatcaa cggtcagtct
    17221 cttggcacat ctgtggtaga aatagatgaa tatcgtttag tcgtacgtga tcagtatggt
    17281 ttttatctta tcttttctta tcccgaacaa actctttatg acgaatctgc agacacttca
    17341 tataagttgt tatctgaaaa atagatccct cccaatgtat atatatatcc tttataaacg
    17401 aaagaatgag tcttctctat catctttcgt tttttgttgt aaaaacgtct aaaaatcctt
    17461 tgttttttat ttagaaaaat agtcaattaa cgtttttttt gttacactta ctaatgatgg
    17521 ttttttacca tgaaaggatg atcgaatatg acaccaaatc gagaagatta tttgaaaatc
    17581 attttagaac taggtggaga tacaacgaaa gtcaacaata aacaaatcgt atctagtcta
    17641 gatgtctcag ctgcatctgt cagtgaaatg atttctaaac ttgtgaaaga agagcttgta
    17701 gaacattccc cttatcaagg tgtacaattg actgcaagcg gactgcaaaa agctagcagt
    17761 ctgattcgca aacatcgctt atgggaagtt tttttagttg aacatttaga ctatagctgg
    17821 aatgaagtcc atgatgatgc tgaagtattg gaacatgtga cttcggaaca tcttgcagat
    17881 catttagaaa actatttgaa tcatcctgaa cattgtcctc atggaggatc gattcctaaa
    17941 aaagaggaag ttgtccatga agaacgccga caaacattgg agtcctatcc tgtcggtaca
    18001 aaagtgcgta tcgcacgagt gcttgatgaa aaagaattgt tggattactt agtcactatt
    18061 gacctgaata tcaacgaaga atatgagatc gttgacatcg cagcttatga aggaccgatc
    18121 actatccaaa atgatagaaa gaaaatcccc gtcagcttta aggcggccag cacgattttt
    18181 gtcgataaaa tataaaggag tgtatccaca tgtctaatga aaaaaatgta ttgtttacga
    18241 tattcggcgg tacaggtgac ctagcccagc gtaaattgta cccgtctttg ttccgccttt
    18301 acagaaaagg aaatttaggc gagcattttg ctgtaatcgg aaccgctcgc cgtccctgga
    18361 gcgatgagca ctaccgtgaa gttgtcaaag aaacaatccg tgcattgaat ccttcagatg
    18421 aagaagcaac tacctttgca agccattttt actatcaatc ccatgatgta aatgattcaa
    18481 cgcattatca gacattaaaa gaattgtctg accgattaga tggacactat caattaaacg
    18541 gtaaccgtgt ctattatttg gctatggcac ctcaattttt tggaacgatc gtttctcatt
    18601 tgaaatcaca aaatattatg acaaaaaatg gcttcaatcg tttgatcata gaaaaaccgt
    18661 tcggttctga ttaccagtct gcttttgaat tgaaccagca aatcagagaa gtattcccag
    18721 aacaagatat tttcaggatt gatcattatt taggaaaaga aatgatccaa aacatctcag
    18781 ctatccgttt tgccaataat atttttgagg ctcaatggaa caatcgctat atcgacaata
    18841 tccaaattac ttttggcgaa aacctcggtg tagaagatcg cgggggctac tacgatcata
    18901 gtggcgcact taaagacatg gtgcaaaatc atattttaca ggttgtagca ttacttgcaa
    18961 tggaaccgcc agtagcattt tccgaaaaag aaatccgtac agaaaagatc aaagcattga
    19021 aagctatccg catttataac gaagaagaag tgttagaaaa cttcgttcgc gggcaatatg
    19081 ctcaaggtga attggatggt gtacaattca agggctatcg cgaagaagac aaagtggatg
    19141 ctcaatcaga gactgaaaca ttcgtcgcag gcaaattcac tatcgataac ttccgttggt
    19201 caggtgttcc tttctatgta cgtacaggca aacgtttgac tgaaaaaggg actcggatca
    19261 acatcgtatt caaacaagtt cctgttaatg tattcaaaac atctgtcgat gaaccatgtg
    19321 acgatacgac acttccacct aatgttctaa cgatctatat tcagccaact gaaggatttt
    19381 ctttatcctt gaatgggaaa gaagtagggc aaggatttga aacggaacca atcaaattgg
    19441 aatttagaaa tagtgcagaa atggttgaaa atagcccaga agcttacgaa aaattactat
    19501 tagatgcgtt gaatggtgat ggaacgaact tctctcattg ggaagaagtc gctcaatctt
    19561 ggcatatcgt ggatgtcatt cgaaaagctt gggaccaaac acaaccggat tttcccaact
    19621 acaaagctgg atcaatggga cctaaagcag ctttcgaact acttgaaaaa gacggattcg
    19681 aatggatctg gcaaccagat aactggtatc gtgaacgtgg aaaattaaac gattgataac
    19741 aaaagggggt gtgacaaaga acatgtcaca cccccttttc ccttttactt ttcttcgaat
    19801 ccttcagcta cagcttcagg atagaagtct tcaacgattt ttgcacaacg accgcataga
    19861 tgaggaagtt tttcatctac tcctacatct ttacgaactt ggcgacagcg atcgcaagtt
    19921 tccccgtctg ctttttccac tgtaatagca acatcatcaa ataccatagc gttatctggc
    19981 acttgttcat ctgctgccat aacttcaaag tagtctggtg aaacgatcaa taattgtgca
    20041 atatctgcat ccaaagcttt taacatttca cgaacttgtt gttttggata aacagttagt
    20101 tttgcttcca aagatttacc aatcaatttg ctgtttcttg cttcttccaa tgcttttaag
    20161 acattatcac ggaagtccat gaaagcagtc caggtatcca tcaattcttc ttggtttgca
    20221 aactcttcat aaccaggaaa ctcgcttaac tgaacgtact cctcttcttc tttcaggaat
    20281 gaccagattt cttctgctgt atgcggaatg ataggagtca ataattttgt caaagctaca
    20341 gctgtttgat agaaaacagt ttgcatgcaa cgacgttgat aatcatcttc tgcttcaata
    20401 taaactacat ctttagcaaa atccaggtag aatgacgata gatcaaccgt caagaagttc
    20461 ataactgtac ggtaaatatg catgaagtta tatttgtcgt acccttcttc tcggatttct
    20521 ttgattactt ggttcaatcg aacggtcata tatttgtcta cagaacgtaa atcattgtac
    20581 gaaacagtat cttttttagg atcgaaatct tctgtattcg ctaataagaa acgcatcgta
    20641 tttctgattt tacgatatac ttctgccact tgacttaaga tatccataga aacacgaaca
    20701 tctgcttcat aatctacact agatacccat aagcgaagga tatctgcacc aaattgtttg
    20761 atcacttttt ctggtgcaat gatgtttcct aaagatttac tcatcttacg gccttcacca
    20821 tctaaagcaa atccttgaga aataactgat ttgtatggtg ctacaccatt gatagccaca
    20881 ctagtcgtga tacttgagtt gaaccagcca cgatattggt ctgatccttc taaatacata
    20941 tctgctggga atgataattc tggtcgctga cgtaaaacag cttcatgaga agaaccagaa
    21001 tcaaaccaga catccatgat atctttttct tttgtaaatt cgccattagg agaacctggg
    21061 tgggtaaatc cttcaggaag aagttctttc gcttcttttt caaaccagat aattgatcca
    21121 tgttcagcaa ataattttgc cacatgttcg attgtttctg gtgtgatgat tgcttctcca
    21181 ttttcagcat agaaaatcgg aagtggtact ccccatgcac gctgacggga aattacccaa
    21241 tcgccgcggt cacggatcat attatacaaa cgtgttttac cccatgggat aacccactct
    21301 actttttcta cttcatccaa aatattctga cggaatttat cgatagatgc aaaccattgt
    21361 ggagttgccc gatagatcac aggctttttc gtccgccaat cgtgaggata actatgagta
    21421 aagaaatcta gcttcagcaa agctcctttt tcttttaata gatcagtgat cataggattt
    21481 gctttgtcat aaaagatacc ttcaaatcca ggtgcttctt ctgtaaacaa tccttttgag
    21541 tctacaggtg acactacaga tagtttatat ttgctaccag cgatatagtc atcttcccca
    21601 tgtcctggag cagtatggac taatccagta ccagcgtcta aagtaacatg atcgcctaaa
    21661 atcacaagtg attcacgttc atagaatgga tgacttgcag tcatatattc catttcttgt
    21721 ccagcaaatt cttttagaac tgttacattt tcccaaccga tttctgattg gactgtttct
    21781 agcaaatctt tcgctacgac aaacttacgg ccatcggctt ccaccaatac atacgtaaat
    21841 tttggattta cagcaatacc ttggtttgct ggcaatgtcc atggtgttgt cgtccaaatg
    21901 acaaaagctg tatcttcatc aagaatatct ttgccgtctt tgacattgaa agctacaaaa
    21961 atagatggag attttacatc tttatattca atttcagctt ccgctaaaga agactcactt
    22021 gacggagacc agtaaattgg tttcaatcct ttgtaaatat agcctttttc agccatttta
    22081 ccaaatacgc gtacttgtgc agcttcataa gaaggatcta atgtaacata aggatgttcc
    22141 caatctcctg caactcctag gcgtttgaaa tctgctcgtt gcttatctac ttgggaaagt
    22201 gcatattcac gacatttttc caagtattct gccatggaca tttctttacg tttcacgcct
    22261 ttattagcca atacttgttc aatcggcaat ccatgtgtat cccatcctgg aacatatggt
    22321 gcacgatagc cagacattga ttttgaacga atgataatgt ctttactgat tttattcaat
    22381 gaatgtccta aatggatatt accattggca tatggaggtc catcatgaag aacaaagcta
    22441 ggttttcctt cgttcaactc ttgtcttttt tggtagattc gattttcttc ccattctttt
    22501 tgccactcgc cttctcggtt tggcaaatta ccacgcatag ggaattttgt ttttcctaat
    22561 tgcaaggttt ctttcatttt cattcaacca tacactcctt tatttgtttt ccggacacaa
    22621 aaaagcatgt tcagccgcaa gggacgaaca tgccgtggta ccacccaaat ttagttgcat
    22681 ctatctagtg caaactctct gtgaccaata acgaggtcag ccgctgcttc ttactctaat
    22741 ttcggaaaag caggtctcaa gtagaggatc tttcaccatg cagtgtctta ccgaacttcc
    22801 actctcatcg gctcgcttta agaataacaa agtgcttttc tgttctactc gttttcattt
    22861 tcaccaggca cgatcgagat atcaatagcc tgtgtatcaa atgccgtcat aggtgcttct
    22921 atgactcctg tgtctgagtt tacatcagta tcattttcat tgtcaagctg ttttgctaaa
    22981 acttctttga tcacttcatg gctgtcagtc acgtagctgg agaatggttg taaaatttca
    23041 tcccattccg gacttttcac ttgttctaat tgagactcaa gcatcaagct gatacgttga
    23101 tggaatactc gtgttttttt cttaagatct tcagtttctg tcgcaagctg tcttgctttt
    23161 tctgttgcat ctgtcaagat ttttcttgct ttttcttctg catcagttgt taattgatgt
    23221 gcacgttttt cagcgttcgc aactaactca tctgctttgt tttgtgcaga agtaacgatc
    23281 acttcggatt ccttgcttgc acttgttttt actttatcag ccgtatcttg agctacgatg
    23341 attgattggt tcaaagcatc ttttaattca ttgaagtact caagtttttc ttcagaatgt
    23401 ttgactgctt tttctaattc tcgatttctt tgagtcaatt cctcatagtc acgaacaact
    23461 aaatctaaga aatcatctac ttcgtcttga ttatatcccc gcatttttgt cgggaatgtc
    23521 ttattttgga tatctaatgg agttaaagcc atgagtaaac caccttttta ttttatttac
    23581 gtaatacacc gaacagtacc ctgtactttt cttttttcgt cttcccctca agttcttgga
    23641 tctggactct gccataacct ctgactgaaa taatatctaa tagttcaagt acaaaatcag
    23701 gtcttgtatt ttctgtccag ttcactttta ctttaccagc ttcgatcagc tgcttggacc
    23761 gctgtctgga aatattataa atctcagaga tcacactgtc tagtcgcatg gaactaactg
    23821 ttcctctttc ttgtgtccag ctatctttcg gcataatcat ttctgtgtat ttgattggtt
    23881 ccaagcgtac agcaaccttt ccaatctttg ttacttgtgc agcaacaaaa tgatccacct
    23941 catttgcaat aaatacttgc cagcgatcac cgtccgagat gatatcgcca aagtaagagc
    24001 gtttgatccc cgttcccact aaagttccaa ggattttccc atgacttagg ctggcaaatt
    24061 tgctaggata atggatttca tacaatgctg tttcaaaatc ttcctctaca ggttcgtaat
    24121 aatcggggaa aatcaaacat ctttttcttt ctgcctgttc atatccgcca taaaacatga
    24181 aacgcaaatc actgctttgt cgaatcagtg tttctaaaat ataggattgt cttgggtcaa
    24241 gaaattcggt cagatagggt gcatattgtc cttgaacctg ttcaatccaa tcgcctaccg
    24301 aatcgataaa tgtacgttca tctgcccgaa agtgctggta tacatttgca tccaaaattt
    24361 tcccctccta tctattctga gaataaagat tttgttgctt caatcaataa actaaatgga
    24421 cagttatcca tataacgatc tgtgctaaac cttgtacggc taagttcaag atcaggattg
    24481 cccctaaaat tgtgaaatcc aagggtccaa tcctcaaagg cagacgatcg aacaaactga
    24541 tatacggttc gcagatctta gctaataatc gtcctagccc tgattgatac ccgcctggaa
    24601 accaagagag caatgcatat accatcaaga taatgctata gaaataggct gcccgttgaa
    24661 gtaaatgaag taatagtgca atagtataaa tcgtagttcc tcctcttaca gatcaaacaa
    24721 attcgcatct gcaaaagatt gagcggttga actgtcaatc tccatcccag tcggtgtaca
    24781 aagaaaaatc tcatctccaa cacgtttgat atctccatct agtgcataaa ctgttcctgt
    24841 tagaaaatca acgattcttc tcgcttgttt ttcttctact aaatgaaaat tcactaaggt
    24901 tgcttctcca gcaatgattc gtttagcaat cgccatagct tcagaataag ctcttggttc
    24961 cattacagtg atttttccag caagatttgc tttaggttgt gtagaggttc ttgaagctgt
    25021 tctttctttc gctacagttt ttgtaggtga ttgatgtagt tcaattactt ttttcttttc
    25081 agccggctga atactttcat gcgttacagg acgtgaagtc gccgtttttt gtttttcatg
    25141 agtattcgca gcagatacag aatgagttga ttgattctgt gttgtatttt ttttagttgg
    25201 ctgtacagcc ttttttgttg tttcaggata atcttggtat tcgtactctt cctcatcagc
    25261 taatccgaaa aaattagcaa ttgctcctct gtttaaaagc ggcatttttg ttcctccttt
    25321 attcgtcttt gaatagcgct gttcctacac ggataaatgt tgcaccttct tcaatcgcaa
    25381 tttgaaagtc ttgactcatc cccatactta attcactaca tggtgcatgg gcataacctt
    25441 ttgcttgaac aagatcacgc aattctttca gcgtaccaaa cactttatgg atttgctcat
    25501 cagaagcttc tttaggcgcc atagtcatca acccaacaac cttgattttt gtgcaggcag
    25561 ctaaagattc cacaaacggt aaaagctcat ctggtgaaat tccatgtttt gattcctcac
    25621 cagaaacatt tacttcaata aaacaggata attctttctc taaccgcttc tggatttctt
    25681 ctgctaatct taaactgtcc aacgcatgaa agtaatcaat ttcatttaca atcaatttta
    25741 cctttctgcg ctgtaaatta ccaattaaat gccactttat ttcaggaaaa tcaaataaat
    25801 cttgtttctt ttctaataat ttatctactc ggttttctgc acaattcata acgccagctc
    25861 taaccaattc ttcagcttga gcggattcaa cagattttgt tacagctact aatgtaactt
    25921 ctttcggatc acgatgaaca agagcacacg aatcctgaat ttcttgcttg attttctgca
    25981 agttgtcagc aatcatgtgc tcttcctctc ctttattttt tacgacggaa aaacggtggc
    26041 gtatttaatt catcatcgcc atgagatttt gtttcttcgc ggttgaatgt atcaaattct
    26101 tttttttcga tattgtcaaa attggaatca tcaactctag gacgaacatt ttgttcacgt
    26161 ctgatatccc aatcgccaaa actgttttct tcttctgaag aagttggttt agcttgatcc
    26221 atatctaata cagtcttttg agcaggtgat tgcatttgtg cttgtctagc tggtcggcta
    26281 gacttgcgtt ccttttttga ttcatcgata cctgttgcga tgaccgtcac acggatttca
    26341 tcgcccattt cttcattgat agatgtacct agaatgatat ttacatcgcc agttgctgca
    26401 tttgctacga tatctgaagc atcttgtgct tcgaacaatg tcatatccaa accgcctgtg
    26461 atgttaagaa gtacttgttc agctccatca atagatgttt caagcaatgg agaggagatt
    26521 gcttttttcg ttgcttctac tacgcgatct tctccactag ccacaccgat ccccatcaat
    26581 gctgtacctt ggttttccat cactgttttc acgtcagcaa agtcaaggtt aacgtaacct
    26641 ggcgctgtga tcaaatcaga gataccttgt acaccttgtc gtaaaacatt atctgcttca
    26701 cggaaagctt caagcatcgg tgtcttttta tctacaactt ctaataaacg gttatttgag
    26761 ataattaata atgtatccac attttctttc aaacgagcga taccttctgc agcaaaacgg
    26821 ccacgtttcg gtccttcaaa agtgaatgga cgtgtcacga caccaacagt caatgcgccc
    26881 aattctttcg cgatacctgc aacgattggt gcagcacctg taccggttcc tccacccatt
    26941 cctgcagtga tgaaaatcat atctgcaccg tctaaagctt cgcgtaagga ttgttcactt
    27001 tcttctgccg ctttttgtcc aacttctggc tgagaaccag cacctaaacc acgagtatat
    27061 ttggggccta attggataac tgtttcagct tttgaatttt ttaatgcttg cacgtctgtg
    27121 ttggctgtaa tgaattcaac acctttaacg ttttcttcaa tcattcggtt tacagcattt
    27181 ccaccgccac caccgactcc gatgactttg attactgcgc cgtcattgat attattatca
    27241 attgagaatt ccatatctta ttcctcccag gatgaattaa tcaaaaatat ttgtaaagaa
    27301 acccttgatt ttatttttta cattttctcc ggactctttt ggttccggct catcgtagat
    27361 tgtttcttca ggtgcttcat agctctcata tgtgtttgtt gtttcttgtt caactaccat
    27421 ttgacgagct gctgaggttt cacccgtaac agcaattttt gccaattgat acacttcact
    27481 caagtttgct gaataatcca cgatacttat tacatttgta aataccgggt ttctcagccc
    27541 catgtgattt ggaacataca attttacatt tacgccgaag atttcttgtg ccaagtccac
    27601 aacaccagga agactagcag ctccgcctgt caatacgacg ccacctggca actctaatgc
    27661 ttcgatttgg tccaatgcat cttttgcttt gttgaaaatc tgttccattc gagctgaaat
    27721 aatttctgac aagtagcgtt catcgacttt cactggttct gactgaccaa tcacatctac
    27781 tggaaattct tcatctggtg atgtccgttc tgggtatgca tccccgtagt ttattttcaa
    27841 cgcttctgca ttgttaaatg acgtatttaa tacaatagag atatctttag tgacaaattc
    27901 gccaccttct tggtcaagat tggtaaactt caattgctta tcatgcataa cagccgttgt
    27961 tgtttggccg ccacccatat caatgacgat cgtaccaaaa tctttttctc cgtcagaaag
    28021 aattgattca gtcagcgcta aaggagtaat caccatttcg ttgacgatta atccggcatt
    28081 ttccacgcat ttacgtatgt tatggatgat cgttttaggt cctgtaaaca acagaccgta
    28141 catttctaat cttacgccga tcattccacg aggatctttg attccctcga acccatcgac
    28201 agtaaaatct tgtggcaata ttgacacgat ttgacgttca ggaggtattg aacgaacaag
    28261 ggcagctgaa gccacatttc tgacatcttc atcggtgatt tcctttgaat ctccgttaac
    28321 tgcaatcatt ccctggcaat tttctacttc aagcatgttt gctggcaaac cgacacttac
    28381 gcctttgatc tggatacctg ctttttcttc tgcttgtcgt actgctcgtt gtatcgcttg
    28441 gaccgtttta tcgatatcaa cgataatgcc tcggttaatc ccttctgatt ttgcgtttcc
    28501 tactccaata atattcattt ggctgtcgat atattcagcc acgacaactt taacagatgt
    28561 cgttccaata tcaaggccta catacattcc tgtttttgcc atgaatggga ttccctccta
    28621 cttactattc catatacaag cgaacaacta gcgtccgttt taaaatatac ttattaatag
    28681 atatattcac tgcttccaat tctaccataa attactggtt tttagaaagt acattcttac
    28741 tttttaaagt tgttttatca attttcattt aaacttgacg gagttgtact tggattatct
    28801 tctatttgac ttgtttcact tgaatcattt gaatcagatg atccgtttgt gctagacgat
    28861 ccaggtgttt gtgctttttg cgtttctgcc tcttcacttg attcgtttgt ttgatccgca
    28921 ttttcttcac tttcagttgt ttcttccgat tggcccgatt cttccgtttc ttgagtgcta
    28981 ccgtaaggat gcgagaagat accaacttcc atgtcgatca cacctttttc atccatatct
    29041 ttggctactt gcgaataata tttcatttgg ttcgcaagat tattgatatt tacaataact
    29101 tgattgccgt cattcatatt caattggatc agatttttat tgcttgctct tggcgtgtac
    29161 ttgatttcag aaattgcttt ttgtaattct gatgataact gattgtattg ctttgttaat
    29221 tctttgattt ttgcagagtc tttgaactct tccaagatag ggagattttt tgtaggattt
    29281 gctacttttt catctaatac tgttccattt tcaatcactg ggtagtattg attgtttttt
    29341 gcgatcaaag caatctcttt atattctgtt acatccaaat cgaattcatt catccctttg
    29401 aaatgtatat ttgctgtttc gattcttggc tgtgcttttt taaggcgatc gatataagta
    29461 gatcgatgaa aatactgtcc ccaaacattt tctcctaatg acaattttgt atcagagatg
    29521 atttcttggc tgttgactgt cttattgcct gatactgcaa ctgcttgtaa tttgctataa
    29581 ggagaaacat aatagatcaa aaaaatcagc ggtataccca atacagtgat gatcaaagct
    29641 aaccgacgaa atagtacttt gtgccgttga ctttttaaat tgggcagccg gttcaaaaaa
    29701 gaaccgttat atggtccacc gtttttcttt tgcttgatct cttcaaaaaa ttcgattttt
    29761 ttctcggact ggctttcttc ttgctgttcc tcttgaaccg ttttttcttc ttcagaatct
    29821 gaaaggggtg tatctaatgt tatgtcttct ttttttggct cttggttttt catctcttgt
    29881 tctgccagat attttcggtt agccttttgc caaggagtca aattgttttc atcagaagac
    29941 ggatgagaca atttttttgg ttcttttttc ttactcacta agaccaccgc ctttattttt
    30001 tgattatttg ttttactaag tcataaagac gttttgaagc atcagggatt ccttgttctt
    30061 tagaagctcg gcacatttgc ttttgcaatt cttcatcacc catgatttcg ttgactgtct
    30121 gacttagtga ttcacctgtt agttcattat ctgcaatcat ttttgctgct cctgcgtgaa
    30181 caagactcat tgcattttta gtctgatgat cattcgtaac atatgggctg ggaaccaaca
    30241 ctgccggtaa ccccagagct gtgaattctg cgatcgaagt tgcaccagca cgccccacca
    30301 gcagatcact ggaagccatg acctctgcca tatttttgat ataaggctgg acactaatat
    30361 tttgtatttc aggatttatt ttatttagga tctcttcata atatctatct cctgaagcat
    30421 acaaaacttg gtaagaagaa ggaatatatt cacttaaaaa aactgatact gcttgattga
    30481 ttttaagtgc tccttgacta ccgccaaaaa tcaagactgt tttcttttct ggatccaaat
    30541 caaatgtttt caaaatatct gattttttag tatctgctac ttcttgtgct cgaggattac
    30601 ctacaagaac tgctttgttt tcaggaaaaa aggaagccgc atctttaaat gaaagagcga
    30661 tccgatccac atatcggctt aaaaatttat tggtaacacc tggtacactg ttttgttcat
    30721 ggataactgt tggaataccc attttggctg cagcgtacac taccgctccg gaaacatatc
    30781 ctccagtacc gataacgata tctggttgaa aatcactgag gatttttttt gcttgacgga
    30841 tactcttaag aaacaatcgg accgtcttga tgttatgcat ggaaattttt cgtttaaatc
    30901 cttgaatctc caaggtatgg aaaggcattc ctgtatcagg gacgattttg ttttctagac
    30961 ctcgttttgc tcccacatac atgaattcag cattcggttc ttgagtcttt acataattga
    31021 caaaggcaag agctggataa atatgtcccc ctgttcctcc gcctgtaacc aaaattttca
    31081 ttttttatcg ctcgcttcta ctttttctat tttaatcggt tcacagcttc catgaatctg
    31141 tttcctctag tttcaaaatt tgggtattga tcccagctgg cattcgctgg tgataataaa
    31201 actacatcac catctgtact gttttctaat gctaaaggaa ctgcttgttc tacagaatcg
    31261 gctgttacta ttgtctcgat gcctgctttt tttccagcat ccatcaactt ttcttttgtt
    31321 tggccaaata cgaccattgc tttgattcct tttaatgaag gaattaattc atcaaatgta
    31381 tttcctctgt ctaatccgcc agccaataac acgaccttag acgtttcaaa tccacttaga
    31441 gccattttag tcgctaaaat attggttgct tttgaatcat tataaaattt tctgccttgg
    31501 atttctccta catattgcgt acgatgtggt acgccatgga aaaaatgcaa cgtttctcgt
    31561 atagcttcat ttgatatacc atacaatttt gctacagaaa tagcagctag tgcattttct
    31621 acattatgct tacctggtac gccaagttca gatatttcca ttattttttc ttttttatag
    31681 tatattgagt aatcgtcagc atacacaccg tcttccaaca cttcttttgt cgagaaaggc
    31741 agtacgcgtg cttttgttct ttgcgctaat tcttgtagtt ctgactgatt ccagttaagg
    31801 atcagataat ctttttctgt catgtttttt tgaaggttcc atttagcttt gacatattct
    31861 tttcttgtac catgataatc aatatgtgct tcatagatat tggtaatcac cgctatatga
    31921 gggcggaaat ctgtgattcc catgagttgg aagctagaca attccatcac aattttatct
    31981 tctgattttg cttcttgcgc tacaccactt gctggatacc caatattacc cgctaaacgt
    32041 gcaattcctt gatctgctcc tgcattcagt agtagtcctg tcatcgttgt tgtcgttgtt
    32101 tttccgttcg ttcctgtaat cccaataatt gggcattccg ctacttcgta agctagctca
    32161 acttccgtaa ttaccggaat acccatttct tgggcttttg ctactaaagg atgactgtag
    32221 ggaatccctg gatttttgac catcaatgaa aattcttcat ccaacaattc gattggatga
    32281 ctacctgtta ttacttttat ccctaaagaa agcagctctt gtgcttccgg attttcgtca
    32341 aaaggttttc catcattaac ggtgacaagt gcgcccaatt catgtaataa tttggccgca
    32401 cttactccgc tttttgcgag tcctaagaca agcactttct tattttcata tgttgatatt
    32461 tttttcattt cccgtcatct cctaccaaac gatccataat gtgatcaacg aagtcacaat
    32521 gctgatcaac caaaaaactg tatcaatctt ccactccgac catccgcaca tttcaaagtg
    32581 atgatgaatt ggagacattt taaaaattcg ttttcctgta agcttaaagg aagtaacttg
    32641 aagcatcaca cttgctgttt ccataacata gatcaaacca atcaaaagca atgtccattc
    32701 ttggtttagc attatagaaa ttgccgctaa aagtccgccc aacgctaatg aaccgacatc
    32761 tcccataaaa atcttggcag gttttctgtt gtagacgaag aaacccagta aacctcctaa
    32821 aacacttaag caaatgatta atacatcata ctgatgctga tgccaagcaa ttagaccgta
    32881 agctgcaaaa gagatacttc ctaagccagc aactaatcca tcgatgccat ctgtcagatt
    32941 tactgcattt gaaaatccaa caagccagaa gagagcgaaa ataccataaa tgattcctaa
    33001 aggtagatct agtccgaaga aattcaatac acctggataa ccttcagaac ggtaaacaag
    33061 ataaaagatg attcccccta gaatctgtcc aatcaatttc tgcttcgagt tcagtcccat
    33121 gtttcttttc ttgaatattt ttataaaatc atcaagaaag ccgattactc cgtataacgc
    33181 taatacaaaa agcaagatga aaagagtagg agttagctgt ttttgccaag ctccaaccca
    33241 aattcctgtc aaaatagagc cgatcagaaa cactaagccg cccattgtag gagttccggc
    33301 tttgctattg tgccactttg gtccctcctc acgaatcgcc tgaccttgct ttttcatttg
    33361 aaaataacca ataaatagcg gcattgcggc aattgtcatt gcacaactgc tgacaatggg
    33421 aatcaatgct tgtgtccatt ccatgctctt ttctctccta tttctcttct tattcttcta
    33481 aagtaaaact aagtttgtct tcagtgattt tttcatatgg agcaagttct tgtttgacac
    33541 aataaccatc tccatcaaaa ctaacttcaa taccgaggat tttccccagc ttcatgatat
    33601 ctgcttttga ccatcctgtt acgtcaggca tcagcttatc accaccagta tataggatca
    33661 gtttttctcc agagatcaac tgatcaccgt tggcagtaga ttgtttttgt acttttttac
    33721 cattaccgat cactacaggt tgcagtccac ttttttgagc atcagctgct gcaacgtccg
    33781 cttcaagatt acgataatcg gctacggata ctttttctgt ttttgtttct gtatctgaat
    33841 cttcttcagt ttctttaaaa tccattgccc gtttcatcaa tggatttgcg attttagcca
    33901 atgccattcg atcatatgtc ttagggtgtt tcattgttag atacaagaca tagtctggat
    33961 cttcagaagg aaccatctcc acaatcgaat acaggtaggc tgtatctcct gtttgatatc
    34021 cccctgtatc agaagcgatt tgggctgttc cgttttttgc cgatacattg tatccaggta
    34081 cactatatac accataagca ctaccgtagt tttcgctttc gacaacatct ctcatatatt
    34141 cacggacttt ttctgttgtt tcttttgaaa aaggttgccc tagaacttct gtttgagttg
    34201 ttcgctcttc tccagtttgg ggatccacca ctttgctgat atatcgaggt tgaatcattg
    34261 taccgttatt cgcaatagaa gtgaaagctt tcatcatttg gaaattcgtc acacctaccg
    34321 cttgtccata agcactcatt gctcggtcga caatatttaa tgtaggtaaa gcaccgttta
    34381 cttcatcatc taatccagaa tgagtacttt gtccaaatcc cagtttttgt aaatagttat
    34441 accatcgtcc gcctaatcgc tgttccagta tgaccatccc gacgttactt gaccatgaaa
    34501 gagcttgacg catggtcagc acaccttttt caccaaagtc atggtcattg atcgtagcat
    34561 cttctacttg gatttttcca gattggaagg tttcattctc gttgaattct ccttcttcga
    34621 tagcagctgc agtagtgaaa actttcatgg ttgatcctgg ttcataacta tcttgtacca
    34681 agaaattacg ccagatagca tcttttccag tcaatccttc cattgtttca ggattgaaag
    34741 taggtcgttg tcccattgcc aagatttctc cggtttttgc tttcatcagg actgctgtca
    34801 attcttctgg ctgatattct tcatttactt gatccatcag tgtttctaaa taactttgta
    34861 gtctgctgtc taaagtcgtg tagatatctt gtccgtcgac agctttttct tcttctgcca
    34921 ctgtacctgg cagaggattt tgaaaattat ctttttgata aataatctta ccatctttac
    34981 cgctcaagat atcattgtaa gcagactcca aacctagttt accaactaag ccattctcat
    35041 ctttgtctgg aactgcgtat ccaataaaat gagaagaaaa gacgccattt ggatacatcc
    35101 gagcctgatg gtcgacaaaa taaagaccag cgacaccttc ttttttcatg tccgcttcga
    35161 tgttttgctt ggtttcttgg gagatatttt tcccatagct accaaattca acttgataca
    35221 gctctttttt tgcgccgtcc tctaaaatat tcagtgcatc ttttttgtct atagaaagat
    35281 tcttatgcag gatttctgct attttatcaa aattcttttc ttcaacatac agttttttat
    35341 ccccagaggt atatgttttt gagagtatcg ctttaattga ataagaggag gcatcttctg
    35401 cgagcgccac accgttacga tcataaatcg ttcctctttt tgcttttact acttcacttc
    35461 cttggtacaa ctgttttgtt ttttcagcta atgaggtacc agcaacatga cctccaataa
    35521 cgatatacga gaaacggacg gcaaataaaa agaacaatcc aatactcgta gcaaataaaa
    35581 tgatacctac ttttttgcga ttgttcatcg gatttagatt tttcttttcc ataaaccgcc
    35641 gaattttatt tttaagactc atttacttca ctttccttaa attgtcgtca ttaatagaca
    35701 atcctttttc ttctgcgatt tgtttaatcc gttcagtacg agaaagttcg tttttttctt
    35761 gttctaaact cgttttttct tgctttttat cattgatttc tgtttgtatt gacgtgattt
    35821 ctttttcagc tccgctgata tttgtgcgca gcataactgt taaaacacat aaagcaacaa
    35881 tagcaatcgc cacgcttgcc acagcaattt tttccaaagc agaaatctgc tttaatcgtt
    35941 ttgccggtga ttgtggaaga atgatttctt gattcgaaga agaatcaggc atttgttgtt
    36001 ccggctgatc caccaattca ggtaaatcat attcgtaatc ttgcattttc tttaattctg
    36061 ccatcattct actcctcttt cattcttatt ttttctgcaa tacgtaactt tgcactgcgt
    36121 gatcgattgt tttctgttag ctcttcctct gaaggaagga ttggtttacg agtaatgact
    36181 ttcagttctg gttggaattc gtcaggtatg acaggaagtc ccggtggtag atcttgtacc
    36241 gtactatatt ctttaaacat acttttgaca attctgtctt ccaatgaatg gaatgtgatt
    36301 acgctgattc ttccattgac tttcaataac cggattgcct gttctaaaga ggcctcttcg
    36361 gcaccgagtt cgtcatttac tgcaatccgc acagcttgaa aaattctttt tgcaggatgc
    36421 ccgccttttc ttcttgcggg agctgggatg acttgtttaa tgatttcgac aagctcacca
    36481 gttgtttgga ttggttttgt ttttcgtact cgttcgattt cacgagcaat ttgtttggaa
    36541 aatttctcct cgccatagcg gaagaaaatc tttactaatt catggtagga atattcatta
    36601 attacgtaat aagcggataa aggagctgac tgatccatgc gcatatccaa cggtgcatct
    36661 tgatgataac taaatccacg ttctgcttca tctagctgag gagaggaaac ccctagatca
    36721 tacaaaatgc cgtctacctt atctacatgc tcactcaagc gttcttctaa ttctctgaag
    36781 ttcgctttga taaatgtgac cataccttgt tcgacataag aagctaaacg cagcttggcg
    36841 tgatcgattg ctttttgatc ttggtcaaac gcatacagat gccctttaga acttagttgg
    36901 cttaataaat attcactatg tccggcaccg ccgagtgtac agtctacata aacgccatcc
    36961 ggtttgatgt tcaatccatc taccgtttct ttcaacatga ctgtatagtg ttgaaatgtt
    37021 tcagtcatta gtcagcctct tcctattctt atagtccgaa atcaatcata ctttcagcaa
    37081 tttcgtcaaa gttctcttct gctgctgcag aaaaatcttg ccatcgtgtc tcatcccaaa
    37141 tttcaattcg gttagataca ccgatgacta cgcaaccttt tgtcaaagct gcataatttc
    37201 ttaacgtgct gggtatattg attcgtcctt gtttatcaag ttcgcattca gtggcagccg
    37261 aatagaagaa tcgcacaaaa gtacgtgcgt cttttttagc caacggcatt tcgttcagtt
    37321 tagcttccag attttcccat tcgctcatcg gatagccgaa aagacagcca tccaatccgc
    37381 gtgtcagaac aaacttttcg ccaagttctt cacgaagttt tgagggcacg attaaacgtc
    37441 cttttgcatc aatattatgt tggaattcac ccatcaacat tgaaagcccc ccattcctcc
    37501 actaactaaa attagtctac cacaatcccc cactttctac caccttttct gggaaatccg
    37561 ggaacaactt tttttattca taaacatttt tttaaaaatc aagctgcaaa aagcctttta
    37621 aaagactttt tacagctttt ctattttttt cataggtaag aaagtatgtt cacaatgatc
    37681 aagacagcgt acaagacgat tgtcaacaga aaaaccaaac gccaagtcat cttgaaataa
    37741 cgcggataaa taatttcttc ataataatat gcttggaaaa cagcaataca aattcccatt
    37801 agcaaaacag aaataaagaa aaacggtaag atagaaattg tccctgtatt tctagacaat
    37861 gcatgcatac cgccccacaa aaaaggaatc gccaaatcag gggcttttat tttaaaacga
    37921 cgatttaacg aaaataattt tacaagaaat tggcaaccaa acaaaacgat taccgggtaa
    37981 atataccaaa acaagataag tggtgtgaat gtgcccatga acgtaaactc cttttttatc
    38041 gttgcttcaa gtatactgta tatactttac acaactgttt cattttattt aaatttatgc
    38101 agttatattc tatatcttac aaacacattc catgtattcc aattccctat ccgttataat
    38161 aaaagaaaca ggcaacagga ggaaaatcat gatcaaatat tttacattag ataatgagaa
    38221 actgcgtaat accggaaagg aaactgacca aacgatctgg tattgtgtag aacgtcctaa
    38281 tcaagaagag attcatcagc tgacacatca atttgaaatt ccgtcagatt acatcacttc
    38341 catattggac gatgcagaaa attcccgtac agaagaattc aatcaatcaa agttgacaaa
    38401 gccagcgtta ttattgttac aatatccttt tgctaaaaca agtcctagcg gttattttca
    38461 agtggataca tacccgctct ctattatttt gacgcccaaa aagaagctta tcacaattac
    38521 gaatcatgaa ccactgtttt taaagaaagt ctttagccag acctttccaa aaagtgatat
    38581 ttcgcctaat ctcaatatct tctttcaatt catttggcag gtcacactct cttacaacag
    38641 ttatttagct acgcttcaat ctcaatgcga tactttggaa gccaaacttc aagtatccac
    38701 tgaaaacaaa caactttatc aaatcatgga tatccaaaaa agcctcgttt atttcagaga
    38761 agcgacttct gctaatttag atgcattgaa agaactagcg caaaataaac ttttccaaga
    38821 aaatcatgct ttgaaaaatc atttacgtga tgtattggtg gaaacagagc aggcaatgac
    38881 tacttctcgc attcaattaa aactagtaga acaaatgaat cagacttttt cagcaatcgt
    38941 atcgaataat ctgaataata ttatgaaaat tttaacttct ttaacgatta ttttaactat
    39001 accgacaatc gtcggcagta tctatggaat gaacattaag ttacctattg ctgaaaggga
    39061 tgatgccttt atatggctag ttctattaac attatttatt tgtatcgtaa ctacctatta
    39121 tttaaaaaaa gggaagtttt tataaaaaag ccctcttgtg gaaatggaaa aaagattgta
    39181 cacttgaatc aactaattaa tgacgaggtt gaaaacaatg cgtgaaaaaa aacaaacaag
    39241 tacttttgct aaagtaacaa aatttgtcat ttggacaatg ctgattttaa cgattggatc
    39301 tgttgtattg actgctgtaa tgagtgtcat gtaaaaataa aatatagcta tcagctgctg
    39361 aaaaggacct gaaccactgc gaggtggctc aggtcctttt tgcgagtttc aatagttctt
    39421 ttgcatgttc gagtgtgagt tttgtaatct cacttccagc cagcatacgt gcaatttctt
    39481 cttctctttc ttttgttttc aactctgaaa cagaagtttg cgtacgtcca tcaatcactg
    39541 ctttcacaat ataatattga tagtcggcga ctgcagcaac ttgaggcaga tgagtgatgc
    39601 acaaaacttg agagtatttc gatatttgag aaattttatc tgcgatggcc tgtgcaactc
    39661 ttccgctcac acctgtatcc acttcatcaa aaataatact tgtcacacct tgctcagaag
    39721 aaaaaatagt tttcaaagca agcaacatcc gagacagttc gccaccagaa gcgactttca
    39781 ccaaaggctt cagtggctct cctggattgg tcgtaatgta aaattcgatt tggtcaaatc
    39841 catcatgatt caattgtcta ttttcttctt ttaagaaacg tacctcaaac tcagtgtttt
    39901 ccatatacag acttttcaat tcatgcagaa tggatttttc caattctttt gcgatttttt
    39961 tacgcgcttc atgaagatct gcggcttgtt gctgaagtag aagctctttt tgctctagtt
    40021 cctctttcat tttgtccaat tgaccttctg aaaaatcaga agaagctagc tcttcggtga
    40081 tttcgtcata gtaagccaat attgactcaa tggattcgcc gtatttacgt ttcaactgtc
    40141 ggattaattc taaacgttgc gtaacttctt ctaaacgact ctcatctaac tccaataaat
    40201 caatctgacg actcatatcg ccgattgcat cttgcagcaa atagtaagca ctttggatat
    40261 tttcataaat tgcttcatac tccacatcta gatgggcgat cccttgcagt tctgaaacgg
    40321 ccaagccaac accatctaca ctgttttgtt cattattcgt cagtgctccg taacctgcag
    40381 ctaatccatc agcaattttt tgataattgc taagtttttc tctttcttct tttaatttct
    40441 cttcttctcc gacttgcaaa tcagcagcag ctatttcttc ttgctggaaa tggagcatat
    40501 ctattctctg aacataggat ttttcatttt gctgcacttt tcgtaccctt ttttccagct
    40561 cacgataaga gatatattct tgctgataac tctttttctt ctgttggaaa gctacatctc
    40621 caaaacgatc caataaagca agatgtgatt caggctggag cagttcctga tgttcgtttt
    40681 gtccttggat atctactaaa taactgccga ttcttctcag attagctaat gtgataatat
    40741 gaccattcac tcgacaaaca ttttttccag ttaaggacat atcccgtcga acaatcagat
    40801 tgtcctcatc tgtttcgatc cctaattcaa ccatcagttc agagaatcct tcttgttttg
    40861 gtagttcaaa caacccttca agcacacatt tttctgctcc ttgacgtata taatcgcttg
    40921 aacctcttcc tcctgctaac agacctaaag catcgataat aatggattta cccgcaccag
    40981 tctctccggt cagcgcggtc attccttcgt gaaaagaaag ccgcaattcc ggaataatcg
    41041 caaagttagt aatactgatt tcttgtagca tcacattcgc tcctcaactt aaaggtaatg
    41101 caggaagtct ttcttcaata aaactgcctg gtcttcagtt cgtgtaatca tcaaaacatt
    41161 atcgtcatca ttgataatcg aaaataattc tttcttgtaa cgtttatcaa tcaaatttgc
    41221 tgctgccgaa gcatttcccg gcaatgtttt caaaataaca aatttttcca tttggtcaac
    41281 tgccacgaaa gcgtctttta atagtttttc caatttagta tttacatctt cgtttgtttc
    41341 agctggtatg ctataacggt agccgccatc tgaagccggt accttgatca gtttcagttc
    41401 ctttatatct ctggaaatcg tagcttgagt aacagatatt cctttgcttt tcaggatttc
    41461 aacaaattct tcctgctttc ggatatcttg ctcactcaac aatcgagtaa tcaaacgatg
    41521 ccgatctttt tttctcatta cgtcttcgct ccttagcata ttcttcttaa tcaatgatag
    41581 cttatttcct tggtataata aagaagaatc cgcatacttc tcacttgatt ttcacagtta
    41641 caatttattt atggtcaaac tgttcgtgtg catttgaaac cacttctgcc atattaatag
    41701 ctgaattgat tgttccgctt tcgggtactt tcctcaagtg agccaagaac tcgatatttc
    41761 cttctccccc cgtgatcgga gaataactta attccagcac atcatatcca ccttgtgccg
    41821 caaatgacaa aatgtcttct aaaacttttt gatgggtctg agggtcacga acaattccgt
    41881 ttttccctac agcttctctg cctgcttcga attggggttt gattaaagca atcacttctc
    41941 cctcttcttt taaaatagca tgtaatggag gcagcatcag ttttagtgaa ataaaagaaa
    42001 catcgataac agcaacttct ggaattccta aggtaaagtc ttcgggtttt gcataacgaa
    42061 aattcactct ttccattacg acgactctag gatcttgtct tattttccaa gctagctgat
    42121 tgtagccaac gtctaaagca taactcattt ttgcgccatt ttgcaaagct gcatcagtaa
    42181 atccgccagt ggatgcacca atatctaaca atgtttttcc ttcaacagat agatcaaatg
    42241 ctttgagtgc tttttctaat tttaatcccc ctcgagaaac ataaggaagt tttttacctt
    42301 tgactcttaa ttcactactc actggaattt tttctcccgg tttatcaaac cgctcgtttt
    42361 tttcgttgta aatcaaacca gccatcactg aacgtttggc ttgttctctt gtttcaaata
    42421 atccttgttt gaccgctaat acatctacgc gttctttttc cattctttcc ctcatttctc
    42481 cacatcaaat tgcttgatca tctggcgcat cagcccgcca tcaaaagaaa aaacgtcctc
    42541 ttctaatttg tctatgatct tgttggcgga taacagctca atctccaacg cctcacgtgt
    42601 cttttctagg cctaataaac gcgggtaagt atttttttcc atacgttcat ctttaccagc
    42661 tgttttccct aatgttttcg ttgtaccaat cacatcgagt agatcatctc ttatctgaaa
    42721 agctaaacct aaatgttcgg ctaatcgttg aagtagcaat aagatttctt caggttgctt
    42781 cgctaatatg ccaccagcca gcaaggcata acgaatcaaa tgccctgtct ttctttcatg
    42841 aataaatgct aattcttcta aagaaagctt tttcgattcg ccctcgatat ctgcagcttg
    42901 cccagcaacc ataccttgtg atcccgcaca aacagctaat tgctgaagca gcagaagctt
    42961 aggagaatta cctaagtgtg ccatactgat tagctggaat gctcctgtaa gcaaaccatc
    43021 tccggccaat atagctaatg cttcgccgaa tactttatga ttggtaggtt ttcctctacg
    43081 aagatcatca ttgtccatcg caggcaaatc atcgtggatc aaggaatacg tatgcaccat
    43141 ttctaaagca gctgctactt gatagacggg aacatcaata ggctcatcaa aggaagcaac
    43201 ggccgttaaa agtaataagg gacgtattct tttcccgcct gctttcactg aataactcat
    43261 cgcatcatag agtcctctat ccgttgtatg ctcatctaaa aaagacaaga tttctttttc
    43321 tacagcaggt agatgaatag ctgaaaagtc agttaatctc atctcagctt tcctcgtttc
    43381 cgtcaaaatc aacttcttca tttgattctg tcatcatttt cgtcaaggtc tcttctgctt
    43441 tagctaatgt atcttggcaa tgtttgctta attccattcc tcgtttaaac gaatctagag
    43501 ctgattctaa tggaacgtct ccttgttcta actgcatgac aattttttct aattcttgca
    43561 gtgactcttc aaaagttgga ttttccatat ttttattctc ctttgtctat ctcttgtact
    43621 tgtcctttaa cgattccatc ttgataatgg atcgtcagca aatccccttg atgaatctcg
    43681 gtgacacttt ttactacttg atcatttaac gtcgtatagc tgtagcctct tcccatgatt
    43741 ttcaatggac tcaacaaatc aagagattga acagcttgag caaaacgctg ctgctgttgt
    43801 ttcatatatt gttggatttt ctcttctaag cgtttttcta gataagcagt ttcttggcga
    43861 gctgctttaa ctcgataaag cggagccact tgttccaatt ggtggcttaa tgcgatcgtt
    43921 tgtttttctt tcgcatgata aatttgctgt gtagcttgat ataatcgctg tttcaactgg
    43981 tctaatttga tggtctgacc ttcatacaat ctttcaggct gccgaaatac ataggaacgt
    44041 cgagcacgct cgaaccgttc ttgtttgcgc tggatctggc gtacaaacgc ctgctgcaat
    44101 ctagcttgcc gttcttgaat gcgcatcaac tcttctgaca aaacaggaac agctaattct
    44161 gcagctgctg ttggagtggc agcccgaaca tctgctacta aatcagcgat cgtggtatct
    44221 gtttcatgtc ctacagatga aatcaccggc gtattagctt gataaatcgc ccgagcaacc
    44281 cgctcttcat taaacggcca taaatcctca atcgaaccac ccccacgacc gatgatcatt
    44341 gtatcgaaat tgcccatctc ttctactctc tggatattcc tgacaacatt gtccgctgct
    44401 tggtttcctt gtacaactgt tgggaacaaa accaattgga caattggata tcttcttttc
    44461 accgtcgtga tgatatcgcg gataaccgct ccgcttggac tggtaaggac cgcgattcgc
    44521 tttgggaaac gtggcagcat ttttttaggc ccttgaaaaa ggccttcctt catcagcttt
    44581 tcttttcgtt cttccaattc ttgatataaa gcacctattc catcaggctc catgtgctct
    44641 acatagattt gataagaacc tcccgcttca tataatgaaa tacgcccaac gacaaggact
    44701 ttcattcctt cttttggctg gaatcgtaat ttttggaatg ctcctttgaa cataatagca
    44761 gatatttttg catggtcatc ttttaagctg aaatattgat gggcattggc acgtaaacgg
    44821 aaattcgata tttcgcctgt taaatatacg cgttctaaat aaggatcggc atcaaatttt
    44881 cttttcaaat acttcgttaa tgtagtgaca gttaaatatt cttgtgccat ctattcacct
    44941 gctccaaaag cttcggcaga ttttacggtt tgatacatca gcatcgtgat agtcattggc
    45001 cctacaccct ttggcacagg agtgatccag cctgcaatag gctccacttc atcaaatttc
    45061 acatcaccga tcagcttacc gttagcatca cgattcatcc ctacatctat cactacagca
    45121 cctggtttta cgaattcttt tgtgacaaaa tgtccacgtc ctattgctac gactaaaata
    45181 tctgcttctt gtgccagttc agccagattt tcggttttag aatgagcaac agtaacagtt
    45241 gcatctgcca tcaagagtag ctgagccatc ggtttaccga cgatattgct tcgtccgata
    45301 actactgctc gtttacccgc cggatcgata gaataagctt caaacatttt catgatgccg
    45361 tatggagtac aaggaatctt gtccggttta cccgcaagga gtctgcctaa attcatcgga
    45421 tgaaaaccgt ctacgtcttt ttcagggcgg atagctaaca ggatcttttc ttcatctata
    45481 tgctttggca atggtaattg gaccaatata ccatgaaaac taggatcttg attgtatttt
    45541 tcaatttctg caagtagttc gtcttcagaa atcgtctcag gataacgctc taccaatgaa
    45601 tggattccga tttcttttgc tacacgttct ttatttctta catagatttg gctggctgga
    45661 ttttccccta ccaataaaac gactaatccg ggatggatac cattatcttt caatttttgg
    45721 attttttcag caatctctgc ctgcattttt tcagctaatt cttttccgtt aatcaattct
    45781 gccatctaac cactcccaga tattttttgt gttttcattc taccatagaa ttttcccgat
    45841 ttataacagg tttatgatga ttcatagaaa aaagctagga cttacgcccc agcttctaat
    45901 tctttcaata cattggataa aacaccgttg acgaatttac gtgaacggtc atcactaaat
    45961 gtttttgcca actcgattgc ttcatttaaa gcaacagccg ctggtacatc tgtcacataa
    46021 gtcatttcaa aaatcgcgat acgcaagatc accaaatcca tttttgagat ccgattgatg
    46081 tgccaattgt tttttaaatg tttttcaatc actttatcca attcatcttt ttttgcacag
    46141 acaccgccaa ctagcgtatc aagataaaca ggaacaaact ctgattcatc ttcgttgatc
    46201 atgtcgcggt ggtctaattc gattgcgttg aaaatcgcat cttcttttgt taggtctgca
    46261 ttgaagtcta atggaaataa agcctgcaat gccatttccc gaatctcgtg tcttgataat
    46321 tctttattca ttctcttcct cttcgtcttc aaaaagttct tcgattgttg gttgttctaa
    46381 tttttcagga acaacagcta ctacatggat attgacttca gataaatcga tatccgtcat
    46441 gaaaaggact tgctggctga cccgttcctg catttccatt gctactttag gaactgaaac
    46501 accatattct agataacagt aaagatctac tttcaagcca tcttcctcgt ttgctaagta
    46561 aacacctttt ccgtgagctg cacgtcctaa taattcagtg acattgctgg caaaagttcc
    46621 gcgcattccg tatacgcctt ctactttaga agctgcaatc ccgataatta cttcgatgac
    46681 ttcaggagcg atcacgatct ctcctaaatc ttggtttgcg tttaaaatca aatttttttc
    46741 atctgccatt tatttattcc tccctttgcc taagcacgag atacatccga attctctatg
    46801 atgcggcata gtgatcacgt ggtaagaaaa ctgccgccga tcagctgcaa actgctatga
    46861 attggagaaa acacccaact ttattatctc ttacagctta aaagttcttc tatagtgtat
    46921 cattaaatcg agtgattttc tataacaaag tctgtgaaca ctgattttta cgcttacttc
    46981 ttctattgat gaaactataa gatgatcaat tcttttggtg aacgagtcaa cacacgattt
    47041 ccatcttctg tgatcaacaa gtcatcctca atacgtactc caccgattcc tggtaagtag
    47101 atccctggtt catcagtgat cacatttcct ggtacaaatt gcttttctgc acgagaagaa
    47161 acgtttggtc cttcgtggat ctctaaaccg atcccatgtc ctgtagaatg tccaaatgct
    47221 tccccgtaac catgttttga aatataatct ctagcaactg catctaattg gacaccagtc
    47281 attcctggtt ttgcagcatc aataactgct aattgagcat ctaagactat ttgataaatt
    47341 tcttttaatt tttccccagg atcaccaata gcgaatgtac gtgtcatatc ggaaacgtac
    47401 ccttcgtaat agcaaccaaa gtccaatgtg atcaagtcac cttgttcgat gatttttttg
    47461 cttgctacac catgcggcat agccgaacgc aatccgctag caacgatcgt atcaaaagaa
    47521 acacctgaag cgccaagaga acgcatataa aagtctagtt gatttgcgac ttcaatctca
    47581 gtcatgcccg gttggatcat tttcaggatg tgatcatatg ccatatccgc aatatgacat
    47641 gctttttcga taatagcgat ttcttcttca tctttcactt ctctgagttc ttcgatcatc
    47701 tgtgaaacag gtactaattc cgcatcgatg atttcttcta aaaccgaata ttctaaaaat
    47761 gagactgttg tttcctcaaa agctaatgag tgcagttctt ctttgcgtac taagtttgct
    47821 acctcatcaa aaataggtcc gacattttta ataatttcaa acccttgagc ctgcgctgcc
    47881 gcctgctcgg tataacgaaa atcagtaata aaaaatgctt tttccaaagt aataacagct
    47941 aatcctgtcg tacctgtaaa gttcgttaag taacgaagat tgtacggact ggtaaccaaa
    48001 aatgaatcca aattttcttc ttgcattttt ttgcgcaatt tttctacacg tagcatcata
    48061 atgaaactcc ctctttcttt gcacgctttt ttccattata gcaaactttt cttcgtacac
    48121 gacagttaaa taaaaatttc cggaaaactt ctttttattt taaataaata aagccaaaaa
    48181 ggagtcacag tctcgctgca actcctttta gtttactatt ttgtaaaaac ttatgatcgt
    48241 tttccaaaga aaaatgagac aactgctacc aaaatcactg cgcctaaaat agatggaata
    48301 agtgccatac ctgcagcttt tggtccccat tcgccgaaaa tcgcttgtcc aatacttgaa
    48361 ccgactagtc cagcaatgat attcgttaca cagcccatcg aatttccttt actggtgatc
    48421 gcacctgcaa ttgctccgat cacggctcca acaatcaatg tccataacca actcataata
    48481 taacctcctt ctttgctaat caattttagc atatgggatt taaaggggac aaataattgg
    48541 ttttgctatt tcatattttc ttctgcaaag taaaaaaata aagaaaacct cctcttagaa
    48601 gttttcttta ttttttactt gcgtttaaac atactacgaa taccacgttt cactacatca
    48661 aagaaactca atgattgtac cattctatct tcatctacat attctctgat cgcacgaagc
    48721 acttttttag gttctttgga agaaaatgtg tatgtaccat tttttttcgt ttgaatggca
    48781 tatcgaggaa tccattttcc ctttagcatg actgatgcta gtacataatc cacttcttcc
    48841 catggaattt ggatatattt gcgtctatct cgcgcatggt aaaactcaaa accattatta
    48901 cctatcatga tttgcccgta atctgtcaga cccgtaaacg aagtagcatc aattaccaag
    48961 tctacttttg tattgattga ttgaaccatt ttctccctcc agatatttgt ttatgaacag
    49021 tatactatat aaaaaaagct cgggacaacg cccgagctat atttttatgg attataaaag
    49081 tccgatgacg tgtccaacaa caccgactac gaataaacca aggatgataa cgattggaga
    49141 aacctttttc ttaagcaacc acatacataa caatgttaat cctaatgcag ctaaaccagg
    49201 aatcaattgg tctaagttgt cttgtaatgt tgttaccttt tcaggagaaa gggctaagcc
    49261 attattaact tgttcaaatg cactcttgat tccttcacca cctgaaggca atttatccca
    49321 ttcgatgtaa gcacctttat ctaattttac agttgataca actggtaaga atttgattga
    49381 tacccatctt tgtacaaggg cagctaatac gaacatccct aaaattgaag caccttttgt
    49441 tacgtcttgt aacaatccgc cagaaaggtc atcagtaatt ttagaaccag ctttgtagcc
    49501 aaattcttgt gtataccaca tgaatgccca acgaataatg ttccaaccta agaagaagat
    49561 gattggtcca aggatatttc ctcccatagc taatgaagca cctagtgcac ctagcattgg
    49621 acgtactgta aaccagaaca ctgggtcacc aacaccagct aaaggaccca tcatacctac
    49681 tttaacccct tgaatcgcta catcatcaac aggcgcgcca ttcgcacgtt cttcttccag
    49741 agccaatgta acccctaaaa ttggagaagc aatgtatggg tgtgtattaa agaattccaa
    49801 gtgacgtttt aatgctgcag aacgttcttc ttttgttttg tataattttt tgattgctgg
    49861 aatcatagaa aatgcccagc caccgttttg catacgttcg tagttccatg aaccttgaat
    49921 gaaggtagag cgccacgcaa cagctaaacg atcttttttt gataatttga ttttttcttc
    49981 tgccatttta ttttcctcct tcaagattaa tagtcattca aaatgtcgcc taaagggtca
    50041 ccagagttgc ttccgcctcc attatttgat gaaccaccca ttttagaaag gttcaagtaa
    50101 ataagagcta atgcaacacc taaagcacct agagcaatca atgttaattg agaaattgca
    50161 gctacaacga aaccaataac gaagaatggc catacttctt ttgtagccat catgttgatt
    50221 accaaagcgt aacctacagc tacgaccatt cccccaccga tagccatacc atctgttaac
    50281 catgctggca ttgattcaag gaatgattgc acagtttgtg ctgggataaa tagcaacgct
    50341 gctgctggga ccgcgatacg aaccccttgt aaacatacag caatgatgtg ccacatttca
    50401 accttacgga tgtttccttc ttcagcagcc gcatccatca aatgaacgat tggaacagcg
    50461 attgtacgta cgatcattgt taagaacaac cctgcaacag ccaaaggaac ggcaatcgcg
    50521 atcgcagaag gaacaccttt aacaccttgt ccacctaaaa ctaaaataat tgctgatgct
    50581 actgatgcta atgcagcatc tggtgctaca gcggcaccga tatttgccca acctaaagcg
    50641 atcatctgaa gcgtaccgcc aagtacgata ccagcttcga ggttaccggt aactaaaccg
    50701 atcaatgtac atgccactag tggttgatga aattgaaatt catcaagaat accttccata
    50761 ccagctagaa aggcaactaa tacgactaaa attattgaaa taatagacat gataagcctc
    50821 ctataaattt taatattatt tattacgctt ttgctaattc agcttttgct tttttcaaaa
    50881 tatcatccat gttatcgcgt gaatcattag gtactttacg tacatcgaat ttcacacctt
    50941 tttgttcaag cttctcaaac gcttcaacgt cttcttgtcc catagacaat actttactta
    51001 caacgacttt accgaccgaa tgcgccattg acccaacatt taattctttg atgtctacgc
    51061 caccttcaac agctgtcaat acgtcttcag gattttcaaa taaaagcaat gctttggtat
    51121 ttccaaaacg agggtctttt gctacttcga tcattttgct aataggaata acatttgctt
    51181 ttacacctgg aggagcagct tgttcgatta gtctcttacg cagatcatcc tttgatactg
    51241 catctgaaac aacgataata cggtttggtt gcactgcttt tgtccaagct gtcgcaactt
    51301 gtccatgtaa cagacgtgag tcaacacgtg ctaatacata tttgattttt ccgtctccga
    51361 ccacagttcc ttctggaagt gctcctttag gttgtgcgtc ttcaactgct gctttcggtg
    51421 cttctgccgg ttctaattct tccggtttta cttttacacc gtcttttgct gtttcaatga
    51481 tgtgtgctgc aatttcatga gcagattcca ttgaaaagcg tgatgcgtag gcttcaatca
    51541 acattggtaa attcaaaccg ctgacgattg cccatttatc tttgtgctct tcgaataatg
    51601 tgtttgattg attgaacggt gttccgcccc ataaatcaac taagaataac acttcgtctt
    51661 gattatcaaa agaagcaacc gcttcttcca atttggctct caagtcatct ggaccttcac
    51721 ttggtttcaa aatcacggct ttgacgtttt cttgttcgcc aaagatcatt gaaccggatt
    51781 gtaagattcc ttcagcaaat tgtccatgac ttgctaggat aatccctacc atatttatac
    51841 ctcctattta cttttgttgt aacaaattca aaaatattaa taattgctaa ccattacaac
    51901 agaattattt gaccttttcc aacaaccatt cttgctacac ctttttgatt ttcgctagta
    51961 gttccatcaa aaggattttc ctgtcagcag gtacttttcg aatctctaac tccacttctt
    52021 gactgttcaa gtaacgaaag gaatcaatat cttgttgatt tactgaaaca aaattggtaa
    52081 tcattgattt gccttcagta aaactcatac cgccgatatt caacgacttc aactgtacac
    52141 cgcagcggac aaccgcttca acatcttggg gattggtgaa taaaagcatc acttttgctc
    52201 gtgataataa tccactatga tagatttgaa tcattttctg cacggttatt acatttgctg
    52261 tgatccccgg cggtgcagct tcttttagta gaaactttct caatttatct tgagcgacta
    52321 catcgctgat cacaatgata cgttcaacac ctgtttgctt cgaccaaact gttgcgactt
    52381 gcccgtggat taaacgatca tctatacgtg ccaatcgaat atccatctgc aaaactcctt
    52441 cttcctcact acttcacaga aacccctatc atttctgtgt gaacaaccac ctcctagcac
    52501 acactactat aagcaaaaag cgtgccaact tttttacgat acactaattg aatttaaagg
    52561 attcttaacc aatacacaaa aacgatacac tataaaagtg tatcgttttt gtgtattcat
    52621 ggcacagtgt attctttttt atctaactga ttatggacta gacgtagcag ataatacatt
    52681 tcttctatcg gcacttttac ttttatctga tcttccaaca gaacaactga ggctgccaat
    52741 tggctataaa gaggatcatt cgttgtattt gtcaattcgt tttcttccac agtcaatggt
    52801 tcattccgaa taatacgttc gatcattcct gctgtgtgca tcactaggtt gattttgaat
    52861 ccatattctt catcgccgat tcccgtcgtc tgagagattt gtgtagaaaa ttgccacatt
    52921 ggatcgatta acttactacc attaataaag atgaagttat cagagataaa ctcaacacac
    52981 gtatttttag ctgactcttc atctagggag atgaattcag tttcctccaa ttctgattcc
    53041 atcagcaact gatccaaaat gacttcgata ttttgatcaa taaaacgttc taaaggaata
    53101 aatggggctt ggatattcaa atcagtaatc ccagtagtag ctatgatctg atattctttt
    53161 tggataactg gcagttcttc tttcacttcc acaatcgaca aagctagaac ttctaattct
    53221 gtttcttgac gcttgtttac cgctcgttcg atcaattctt tgattctttg tgctgtacct
    53281 tcgccagatg cgcagatagc caaaacagct tttttcttat gaataggttt tgcttcctga
    53341 acagattcta cagaagatat ttctgcgtaa cctcgaaaat ttttcaatga ttcatgcaac
    53401 atatctagat ctgtccctaa tactgagacc tttcgtacag tttccaaaac tagagaagtt
    53461 gttaccatat caacagtacg tactggaatt ccagtgtctc tttgaatttg cgaattaaac
    53521 gacgcaaggg accccatatc tactaagagt atgaccccgc ttccttcatc tacttcttgt
    53581 acattgcgtt caattcggtc caatgctgtt ttcggatcca tatccaaagg catgtctact
    53641 gctcgtacgt tctctgcatc taacaactgt tggacaactt ggaccatact gcttgctgtg
    53701 ctgtttccgt gagctgcaac gactacaccg attctgccgc ttgcttgatt tgcccgtaac
    53761 gagacgagta gaacggtcaa gtaatagtct tcactttctg gaatctccac ttgaaaatac
    53821 gtttcgatat aactacgcac ttctttcgca acagcatatt catctggaaa atcaaaagcc
    53881 atctcacgga tgttatcatt cgtatgccgt tcttctccta agttgatttt tttcaaaaaa
    53941 gaactgatat gcaagctcat agcataaata aagttttgct gaaactcata tggtaaagat
    54001 cttttgacca ttacataaat ctgattggtc acttctatga ttttcggatc tacaaattct
    54061 gctaatttgc tgtctgcatt aaaagaaaaa ccatggtctt tatagaaaga cttcagatgg
    54121 atgttgatat ctgtagaaat gaaatgattg attgcttctt gatccaaacc atccgacttc
    54181 agtaaagctg ctttatctcc gatgatatca tatagattat aaggtaactc ataggagtcc
    54241 gattggattt tcatgatcgt atcatttggt gctacagtta tctttggctc caatatttta
    54301 gaaagctctg ccattgcact gcggttgctt gctaattgaa tcaatccgct acgaatccct
    54361 tctgtcaaat catcgattgt gatcgagatt tcgggtgatt gcatatgatt tagaaatcct
    54421 cgtgcacata ctaactggat atttgacttc aattgtccta tatttccata ggtcacactg
    54481 ccgatcaatg cttttgctac atcttctgtc aaagaaatct ttcgctgcgt gcggtttgct
    54541 tcgtgggcaa tcatgatttt tactaaatcg actttctcat ttgcaggacg tttttcaaaa
    54601 gccggaagtt tgatattgat tgggatacgc cgaacaaaag tttctaatag agaagaacct
    54661 gggtcttctg tggtcgcacc tacgatccgt acatttgctt catgggattt cgttgtttcc
    54721 ccaagacgac tgtaagttcc atgatccata aagtaaaaga tcatttcttg accttcaggc
    54781 ggcaaccggt gaatctcatc caaaaacagc ataccgccat ccgcttgatc gataatcccc
    54841 attttttctt catctgcgcc tgtaaaagca ccttttgcgt acccaaataa atgactcatc
    54901 aaaagctcag ggttattggc ataatcagca caattgaaaa caatcagttc cgtttcctca
    54961 tctattacat gatgcgcttt tgcaaaatga tacatcgcgt gtgcaaaata agtctttcct
    55021 gatccggtag gtcctgtaat caaacaattt aatcctcttg gcggatacaa aatcgcagct
    55081 tttgcctgtt ccactgaatt tttcatgctg ccgttagctc caatgatttt tgcaaagata
    55141 tccttagtat ctatttggag tgtatttttt tcttcagaga ccggcgtttc tttgtaactg
    55201 actacatgtt ttgtaactgg ctttacgtgc gtaacgagtg tttgatatat gtaacgtact
    55261 ggtcgtccgt ctgtctttat cagttttcct tcgcgcacta gctgattcag atctttactg
    55321 ctatttgttc gctgaatccc taacacttca gcaatttcct gtgttgttac tccttgatca
    55381 tattctgcct ttgttaaatg agcggttttt tctttgacat acatataaat tcggtcaatg
    55441 cgttttgcca atggtatcgc tccttttcta tagactattg tacttaggct tcaaaacata
    55501 atactatgta tttatttctg tttcgtcaac tatttacctg attttgtttt gttgataaca
    55561 cgcggataga tagaaatgtt caatttaaaa gaacgaaaaa tcccgatacg atttttataa
    55621 tggaaacaag aaaaagaaca ctgtttacca tataattatt tcactgatag caaacaatat
    55681 agcttccatc aattgaaaac gatagttatc aaaacatcct gattccaaat tggcaaaaaa
    55741 atattaagtg taaaaagaca aagtaaactt cgtagcctcc actacaactt ttatttttat
    55801 aaacaaaaaa ggctgtgaca aaa
//
LOCUS       DAZUKT010000008        53642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000008 DAZUKT010000000
VERSION     DAZUKT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.008"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..489
                     /locus_tag="ACG816_000483"
     CDS             <1..489
                     /locus_tag="ACG816_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HFS4919399.1"
                     /translation="YLANHEAILSNVHFVEGANFADRGLQISDLSVEGEPMKLFLQGK
                     EFTDTDQIFHEIRLNWKKPLYVECIFENAWQTREYLSILEDNPFSSWDSSVSEEDTEL
                     IERYFQEKEEEAKLKMLYAQIDQALEDGDRDAFLKLSDEVNRKILLQTQKKSNRVSEH
                     NN"
     gene            complement(571..1446)
                     /locus_tag="ACG816_000484"
     CDS             complement(571..1446)
                     /locus_tag="ACG816_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4919400.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1594..1926
                     /locus_tag="ACG816_000485"
     CDS             1594..1926
                     /locus_tag="ACG816_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4919401.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2038..3246
                     /locus_tag="ACG816_000486"
     CDS             2038..3246
                     /locus_tag="ACG816_000486"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4919402.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            3400..3855
                     /locus_tag="ACG816_000487"
     CDS             3400..3855
                     /locus_tag="ACG816_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919403.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            4217..4492
                     /locus_tag="ACG816_000488"
     CDS             4217..4492
                     /locus_tag="ACG816_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919404.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            4505..6583
                     /locus_tag="ACG816_000489"
     CDS             4505..6583
                     /locus_tag="ACG816_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4919405.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6746..8629
                     /locus_tag="ACG816_000490"
     CDS             6746..8629
                     /locus_tag="ACG816_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4919406.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            8641..9588
                     /locus_tag="ACG816_000491"
     CDS             8641..9588
                     /locus_tag="ACG816_000491"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4919407.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9608..10135
                     /locus_tag="ACG816_000492"
     CDS             9608..10135
                     /locus_tag="ACG816_000492"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4919408.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(10198..10851)
                     /locus_tag="ACG816_000493"
     CDS             complement(10198..10851)
                     /locus_tag="ACG816_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4919409.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10983..11825
                     /locus_tag="ACG816_000494"
     CDS             10983..11825
                     /locus_tag="ACG816_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4919410.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11983..12879
                     /locus_tag="ACG816_000495"
     CDS             11983..12879
                     /locus_tag="ACG816_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4919411.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12882..13595
                     /locus_tag="ACG816_000496"
     CDS             12882..13595
                     /locus_tag="ACG816_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4919412.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13615..14133
                     /gene="msrA"
                     /locus_tag="ACG816_000497"
     CDS             13615..14133
                     /gene="msrA"
                     /locus_tag="ACG816_000497"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4919413.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            14130..14357
                     /locus_tag="ACG816_000498"
     CDS             14130..14357
                     /locus_tag="ACG816_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4919414.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            14509..15060
                     /gene="lepB"
                     /locus_tag="ACG816_000499"
     CDS             14509..15060
                     /gene="lepB"
                     /locus_tag="ACG816_000499"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4919415.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(15143..15685)
                     /locus_tag="ACG816_000500"
     CDS             complement(15143..15685)
                     /locus_tag="ACG816_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4919416.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(15922..16320)
                     /locus_tag="ACG816_000501"
     CDS             complement(15922..16320)
                     /locus_tag="ACG816_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4919417.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            16580..17440
                     /gene="ylqF"
                     /locus_tag="ACG816_000502"
     CDS             16580..17440
                     /gene="ylqF"
                     /locus_tag="ACG816_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4919418.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            17433..18200
                     /locus_tag="ACG816_000503"
     CDS             17433..18200
                     /locus_tag="ACG816_000503"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4919419.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKNLQ"
     gene            18256..19113
                     /gene="dprA"
                     /locus_tag="ACG816_000504"
     CDS             18256..19113
                     /gene="dprA"
                     /locus_tag="ACG816_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4919420.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            19235..21313
                     /gene="topA"
                     /locus_tag="ACG816_000505"
     CDS             19235..21313
                     /gene="topA"
                     /locus_tag="ACG816_000505"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4919421.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            21282..22658
                     /gene="trmFO"
                     /locus_tag="ACG816_000506"
     CDS             21282..22658
                     /gene="trmFO"
                     /locus_tag="ACG816_000506"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4919422.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            22856..23761
                     /gene="xerC"
                     /locus_tag="ACG816_000507"
     CDS             22856..23761
                     /gene="xerC"
                     /locus_tag="ACG816_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4919423.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            23798..24346
                     /gene="hslV"
                     /locus_tag="ACG816_000508"
     CDS             23798..24346
                     /gene="hslV"
                     /locus_tag="ACG816_000508"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4919424.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            24360..25760
                     /gene="hslU"
                     /locus_tag="ACG816_000509"
     CDS             24360..25760
                     /gene="hslU"
                     /locus_tag="ACG816_000509"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4919425.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            25778..26569
                     /gene="codY"
                     /locus_tag="ACG816_000510"
     CDS             25778..26569
                     /gene="codY"
                     /locus_tag="ACG816_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4919426.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            26651..27526
                     /locus_tag="ACG816_000511"
     CDS             26651..27526
                     /locus_tag="ACG816_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4919427.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27658..28296)
                     /gene="plsY"
                     /locus_tag="ACG816_000512"
     CDS             complement(27658..28296)
                     /gene="plsY"
                     /locus_tag="ACG816_000512"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4919428.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            28423..28857
                     /locus_tag="ACG816_000513"
     CDS             28423..28857
                     /locus_tag="ACG816_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4919429.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            29023..31068
                     /gene="parE"
                     /locus_tag="ACG816_000514"
     CDS             29023..31068
                     /gene="parE"
                     /locus_tag="ACG816_000514"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4919430.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            31080..33530
                     /gene="parC"
                     /locus_tag="ACG816_000515"
     CDS             31080..33530
                     /gene="parC"
                     /locus_tag="ACG816_000515"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4919431.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            33807..36038
                     /gene="pflB"
                     /locus_tag="ACG816_000516"
     CDS             33807..36038
                     /gene="pflB"
                     /locus_tag="ACG816_000516"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4919432.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            36282..37043
                     /gene="pflA"
                     /locus_tag="ACG816_000517"
     CDS             36282..37043
                     /gene="pflA"
                     /locus_tag="ACG816_000517"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4919433.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            37128..38054
                     /locus_tag="ACG816_000518"
     CDS             37128..38054
                     /locus_tag="ACG816_000518"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4919434.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            38236..39129
                     /locus_tag="ACG816_000519"
     CDS             38236..39129
                     /locus_tag="ACG816_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4919435.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            39263..39388
                     /locus_tag="ACG816_000520"
     CDS             39263..39388
                     /locus_tag="ACG816_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4919436.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(39522..40154)
                     /locus_tag="ACG816_000521"
     CDS             complement(39522..40154)
                     /locus_tag="ACG816_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919437.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(40302..40844)
                     /locus_tag="ACG816_000522"
     CDS             complement(40302..40844)
                     /locus_tag="ACG816_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4919438.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            40979..41368
                     /locus_tag="ACG816_000523"
     CDS             40979..41368
                     /locus_tag="ACG816_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919439.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            41372..42136
                     /locus_tag="ACG816_000524"
     CDS             41372..42136
                     /locus_tag="ACG816_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4919440.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            42265..43212
                     /locus_tag="ACG816_000525"
     CDS             42265..43212
                     /locus_tag="ACG816_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4919441.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            43342..44082
                     /locus_tag="ACG816_000526"
     CDS             43342..44082
                     /locus_tag="ACG816_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919442.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(44373..45041)
                     /locus_tag="ACG816_000527"
     CDS             complement(44373..45041)
                     /locus_tag="ACG816_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4919443.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            45175..45462
                     /locus_tag="ACG816_000528"
     CDS             45175..45462
                     /locus_tag="ACG816_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919444.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            45878..47416
                     /locus_tag="ACG816_000529"
     CDS             45878..47416
                     /locus_tag="ACG816_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4919445.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            47413..48378
                     /locus_tag="ACG816_000530"
     CDS             47413..48378
                     /locus_tag="ACG816_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919446.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            48365..49687
                     /locus_tag="ACG816_000531"
     CDS             48365..49687
                     /locus_tag="ACG816_000531"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4919447.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      49750..49854
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            49928..50671
                     /locus_tag="ACG816_000532"
     CDS             49928..50671
                     /locus_tag="ACG816_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919448.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            50686..51678
                     /locus_tag="ACG816_000533"
     CDS             50686..51678
                     /locus_tag="ACG816_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4919449.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            51690..52427
                     /locus_tag="ACG816_000534"
     CDS             51690..52427
                     /locus_tag="ACG816_000534"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919450.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            52417..53139
                     /locus_tag="ACG816_000535"
     CDS             52417..53139
                     /locus_tag="ACG816_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4919451.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(53223..>53642)
                     /locus_tag="ACG816_000536"
     CDS             complement(53223..>53642)
                     /locus_tag="ACG816_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4919452.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        18505 a         9190 c        11233 g        14714 t
ORIGIN      
        1 tatcttgcca atcatgaagc gattttaagc aatgtccact ttgttgaagg ggcgaatttt
       61 gcggatcgag gactgcagat cagtgatcta tctgttgaag gggaacccat gaaattgttt
      121 ttgcagggaa aagaatttac ggacacagac cagatttttc atgaaatccg tttgaattgg
      181 aaaaagcctt tatatgtgga atgtatattc gaaaatgctt ggcagacaag agaatatcta
      241 tctatcttag aagacaatcc attttcttct tgggattctt cagtcagtga agaagatact
      301 gaattaattg aacgatattt tcaagaaaaa gaagaagagg caaaactaaa aatgctttat
      361 gctcaaatcg atcaagcact agaagatgga gatagggatg cttttttgaa attatctgat
      421 gaagttaacc gaaagatcct tttgcagaca caaaagaaaa gtaaccgggt aagtgaacat
      481 aacaattagt taaaaatagt atagtactaa aaaagacaaa ggtcagctgg atgtggaatc
      541 tttcctcata gcctatcttt gtcttttttc ttatagtttt ttcagtaatc ttgtctgtgg
      601 cttcaaataa gtaaagcctt gcccttccac ttcatgcaca ttgatgattg aaaggaaagc
      661 tttttcgtca atctcgtgga cgatcctctt agcttcagtt aattcactag agctaacgac
      721 catatagatg attttttttg aattaccaga gaatgcgcct tctccagaaa ggaaagtcac
      781 acctctttgc agaccggtca tcagtagtgg tgcaatctct tcgcttttgt tggagacgac
      841 aagtagacct tttgcagaat atccgccatc taagatcgaa tcaatcacac gactgaaaac
      901 atacgaaaca atcaacgtat acatcatacg tttcaagtcg atataggtta atgaagcaag
      961 taagacgatg atatcaaatg ccaataggga acgccccata gtgatgccgt aattttgttc
     1021 taaaatccga gcgataatat cactgccgcc tgttgttcca cccatgcggt agacgatccc
     1081 gcttcctaca cctgcaacta aacccgcaag tagagaaacg atcagtagat catgctgtaa
     1141 attgatttct agtggaaccc tttgccagat ccataaaaag aatgacaaag aaagtgtacc
     1201 tagaacagta taggccaagg aacgtttacc gaggatcttt cctcctagta aaatcaatgg
     1261 gatgttgatt aataaagtcg tataagctgg atcaatacca aataaagctc ttaagatcaa
     1321 ggtgatccct gagactcccc cttctgctaa atgattagcg atgttcaagt aaacaagacc
     1381 aaatgcataa atacttgttc ctataatgat aaaaagaact tcttttactg tttttcgttt
     1441 gaccatatga attccagctt tcaattatgt attttcacag aatttccatt tagaaattcc
     1501 ttcctaaatg tagcatggaa attcgatttt ctcaattgct cttatctagg atttgcgtta
     1561 aagttttggt aagatagttt tggagggaaa aagatggata aaaagcgttc tttatacagt
     1621 atgcagcagg aagtggatga atatatccag caattcaagg tggggtattt ctctccatta
     1681 gctcagatgg ctcgattaac tgaagaagta ggtgaattgg caagagaagt caaccacact
     1741 tatggagaaa aaagcaaaaa ggcttctgaa ccagttaact cggtagcaga agaacttggt
     1801 gatgttttat tcgttacgat gatcatggct aattcattga atatcgactt aactgaagtt
     1861 tttgaaaaaa acatggaaaa attcaatcaa agagaccata atcgctttga gcgtaaggac
     1921 gagtaaacaa gatgaagtgg tcctggcctt gaagactctg aaacggactt gtcaaacagt
     1981 caccatgcaa ctgagtcttt atctcaggac aagaaacacg caggaaaggt tggaaaaatg
     2041 aaattaaaac aattacctct ggaataccaa aaggcaattc cagttctgaa aaaattagag
     2101 aatgcaggat atgaagctta ttttgtaggt ggaagtgttc gtgatatctt gctgaaccag
     2161 ccaatccatg atgtggatat cgcaacaagc gcatttccag aagaaatcaa acagcttttt
     2221 ccaaaaacga tagatattgg gatcgaacat ggaactgtgt tggtattaga aaatgatgaa
     2281 caatatgaga tcaccacatt tcggacggaa tcaacttatc aggactaccg tagaccagat
     2341 catgtcgaat tcgttcgttc gttggcagaa gatttgaaaa gaagagattt tacgatcaat
     2401 gcgtttgctt tgaaagaaga tggagaaatc atcgatttgt tcgatggtct gcaagatcta
     2461 gaagaacaga tcttgcgagc tgtaggcaat cctcatgagc ggttccatga agatgcctta
     2521 cgaatgatgc gcgggttgcg ttttgtcagt caactagggt tcaaattaga ggaagaaacc
     2581 ttctacgcca tcaaagaaaa ccacgagcta ttaggtaaaa tatctgtaga acgtataaat
     2641 gtggaattca tcaagatgct cttgggaaat gaacgggccg cgggaatcca tgcatttatt
     2701 gaaacagagt gctacatttg ctgtcccggt ttgaaagaag aaggcgaagc acttttacgt
     2761 tttgctgaat taccatccgt cccattgaaa gaagagagcc aagcatgggc attattgatc
     2821 agccaactgg atctaaaaga aaatgagatt cgttcctttt tgaaatcgtg gaagtgttct
     2881 aaccaaatga ttaaagacgt tcaagcactt gtccaaggac tacgacaacg agaaactgga
     2941 gctttggaac cattcgaatt gtacttctta ggcaaaaaac atgcactgca agtagaagaa
     3001 ttgatgatct attttgacga aacacctcag atggatgccg taggagaatc ctatgatcgt
     3061 ttacctatca agtcattaag cgaattagca gtagatggta aaatcttgct agaagaaact
     3121 ggaaaaaaac ctggtaagtg ggttagtgaa gctttgcaat tagctgaaaa agccgtcgta
     3181 gaacaaagaa taaaaaatga taaaaaacag gttttggatt ttttatcgaa agaaaaattg
     3241 atttaaaaaa cactagactg tatctgctca gctttgttgg ctacttaaag tgggcagata
     3301 cgtcattaga agaaaaaaca taagatgtga ccacttacat aaatttgtgc taaactgagg
     3361 tagtacaaga ataaattaca tcataaagga gtctttttaa tgagtaaaga aatgacagcg
     3421 ctaaaatttt attttagaaa tggagaaacc tggacgatca accgccgtca tatcggcgat
     3481 ctttggatca aacaaatcac aacaagtttt ggacgaatca atggcagtga atttgttgaa
     3541 atccatccgt gtgctggatt taaaatcgaa atcttccatg aaggggacgc agttgcgaca
     3601 catgatatca acctaggcgg actggaaatg ggcatgttta atcgcgcatt gaaatacgaa
     3661 gatatcgagc gaatggaaat cctttaccga aatggcacac ctgatcttgt gtacttccca
     3721 tatctagata aaggaacaga aggattagac aatcaatatc aatcaacaaa aatcagcgaa
     3781 aaaacaggta acttgtatat cgtgatcaat ccagatcaac gtgtagaaga tgtatacggg
     3841 gaatttttcg aataaatcga tttttacgca aaaatgaggc tgtgaaagag atgtgtctta
     3901 ttactactga taggctgtct cttccacacc gtttattcag aataagggac tgtgacaaga
     3961 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
     4021 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
     4081 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
     4141 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
     4201 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
     4261 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
     4321 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
     4381 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
     4441 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
     4501 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
     4561 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
     4621 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
     4681 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
     4741 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
     4801 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
     4861 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
     4921 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     4981 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     5041 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     5101 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     5161 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     5221 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     5281 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     5341 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     5401 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     5461 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     5521 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     5581 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     5641 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     5701 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     5761 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     5821 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     5881 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     5941 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     6001 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     6061 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     6121 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     6181 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     6241 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     6301 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     6361 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     6421 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     6481 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     6541 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     6601 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     6661 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     6721 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     6781 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     6841 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     6901 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     6961 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     7021 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     7081 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     7141 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     7201 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     7261 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     7321 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     7381 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     7441 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     7501 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     7561 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     7621 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     7681 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     7741 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     7801 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     7861 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     7921 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     7981 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     8041 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     8101 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     8161 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     8221 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     8281 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     8341 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     8401 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     8461 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     8521 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     8581 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     8641 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     8701 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     8761 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     8821 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     8881 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     8941 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     9001 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     9061 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     9121 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     9181 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     9241 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     9301 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     9361 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     9421 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     9481 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     9541 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     9601 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     9661 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     9721 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     9781 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     9841 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     9901 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     9961 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
    10021 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
    10081 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
    10141 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
    10201 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
    10261 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
    10321 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
    10381 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
    10441 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
    10501 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
    10561 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
    10621 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
    10681 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
    10741 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
    10801 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
    10861 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
    10921 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
    10981 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
    11041 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
    11101 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
    11161 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
    11221 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
    11281 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
    11341 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
    11401 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
    11461 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
    11521 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
    11581 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
    11641 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
    11701 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
    11761 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
    11821 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
    11881 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
    11941 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
    12001 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
    12061 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
    12121 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
    12181 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
    12241 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
    12301 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
    12361 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
    12421 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
    12481 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
    12541 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
    12601 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
    12661 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
    12721 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
    12781 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
    12841 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
    12901 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
    12961 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
    13021 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
    13081 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
    13141 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
    13201 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
    13261 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
    13321 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
    13381 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
    13441 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
    13501 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
    13561 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
    13621 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
    13681 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
    13741 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
    13801 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
    13861 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
    13921 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    13981 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    14041 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    14101 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    14161 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    14221 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    14281 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    14341 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    14401 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    14461 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    14521 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    14581 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    14641 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    14701 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    14761 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    14821 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    14881 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    14941 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    15001 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    15061 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    15121 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    15181 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    15241 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    15301 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    15361 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    15421 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    15481 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    15541 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    15601 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    15661 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    15721 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    15781 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    15841 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    15901 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    15961 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    16021 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    16081 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    16141 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    16201 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    16261 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    16321 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    16381 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    16441 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    16501 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    16561 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    16621 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    16681 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    16741 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    16801 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    16861 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    16921 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    16981 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    17041 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    17101 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    17161 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    17221 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    17281 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    17341 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    17401 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    17461 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    17521 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    17581 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    17641 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    17701 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    17761 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    17821 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    17881 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    17941 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    18001 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    18061 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    18121 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    18181 taaaaaaaaa tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    18241 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    18301 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    18361 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    18421 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    18481 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    18541 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    18601 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    18661 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    18721 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    18781 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    18841 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    18901 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    18961 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    19021 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    19081 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    19141 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    19201 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    19261 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    19321 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    19381 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    19441 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    19501 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    19561 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    19621 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    19681 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    19741 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    19801 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    19861 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    19921 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    19981 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    20041 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    20101 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    20161 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    20221 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    20281 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    20341 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    20401 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    20461 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    20521 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    20581 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    20641 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    20701 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    20761 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    20821 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    20881 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    20941 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    21001 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    21061 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    21121 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    21181 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    21241 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    21301 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    21361 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    21421 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    21481 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    21541 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    21601 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    21661 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    21721 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    21781 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    21841 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    21901 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    21961 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    22021 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    22081 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    22141 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    22201 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    22261 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    22321 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    22381 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    22441 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    22501 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    22561 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    22621 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    22681 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    22741 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    22801 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    22861 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    22921 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    22981 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    23041 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    23101 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    23161 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    23221 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    23281 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    23341 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    23401 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    23461 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    23521 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    23581 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    23641 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    23701 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    23761 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    23821 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    23881 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    23941 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    24001 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    24061 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    24121 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    24181 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    24241 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    24301 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    24361 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    24421 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    24481 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    24541 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    24601 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    24661 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    24721 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    24781 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    24841 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    24901 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    24961 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    25021 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    25081 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    25141 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    25201 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    25261 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    25321 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    25381 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    25441 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    25501 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    25561 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    25621 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    25681 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    25741 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    25801 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    25861 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    25921 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    25981 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    26041 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    26101 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    26161 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    26221 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    26281 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    26341 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    26401 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    26461 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    26521 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    26581 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    26641 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    26701 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    26761 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    26821 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    26881 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    26941 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    27001 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    27061 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    27121 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    27181 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    27241 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    27301 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    27361 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    27421 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    27481 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    27541 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    27601 gggagtgtga catcgatttg tcacactccc gttttatctc tttggaatga aaaagtatca
    27661 ggattttttc ttgctgctat tcaaaccaaa cgggattcga ctttcagtat ggtttctgat
    27721 acgcttgata ttgtctttat gccgataaaa gataaaggaa gaaactaaaa aggcaatgat
    27781 cgtcagtaac caattgggct tagccaaaat cacaggagct acataaggaa ggacgatcgt
    27841 agataaagtg atcaatactg cactgatcat actgctcaaa ctcaccatac ttgtacaaaa
    27901 taaactaacg ataaagatga tacaagaata aacaaaaaat acaggattaa atcctaaaag
    27961 cattcctgca cttgtcgcaa cagcttttcc tcctttgaac ccagcaaaaa tcggaaatgt
    28021 atggccaaga atggcgcata ccccaaacca aagagggtta atcccagaaa ggtggaagat
    28081 catcggcaaa caagtagcca atgtaccttt taaaatatcc ataagcagga caacaatccc
    28141 ggctgttttg cctaatacac gaaatgtatt ggtcgttcca gtattaccgc tcccgtgttg
    28201 tcggatatct tttttgaaaa acaatttgcc aatccagaca ccggaaggaa tagaacctaa
    28261 aagatatgct aaaataagta ataagatcaa tttcattttt taccctcttt caagttgaaa
    28321 actatccttt attttagcat gaataggtaa caggaacaat tgaaaaaatg aaagatacaa
    28381 gtataataga tggaataaaa agaaaagcaa ggggtggcag aaatgtctgt aaaaaatcca
    28441 acacaagaaa cgattttcaa ctatttggaa gaagcaaaaa atattgccgt cgtaggcttg
    28501 agcaataaac cagaacgaac aagttacatg gttgcacagg tattgcaaga caatggatat
    28561 aaaatcatac cagtaaatcc attattggct ggtgaagaaa ttctaggcga gaaagtctat
    28621 gcgaatttga cagaaatcga tgatccaatc gatatcgtag atgtatttag acggagtgaa
    28681 tttttacctg aaatagcagg tgattttttg aaaactgatg ctaaagtgtt ctgggcacaa
    28741 ttagggattg aaaacaaaga agcagctgag atgctaagaa aagcaggacg tacagatatc
    28801 gtaatggatc gttgtattaa gattgaactt ggtaagttga aagaaaacca aaaataaaaa
    28861 tgaccacaaa gaagagatga tcgatagagg ggaatgtcga aaaagcggtt aggagataat
    28921 taaaattgat ttctcgtcta ccctcttttt tttcacgacc ttttctagta tgataatcgt
    28981 ggtgcggttt tgccgcaaag tattattgaa ggagcgtttg ccttggcaaa aaaagtaaac
    29041 aacgaataca atgactcgtc catccaagta ttggaaggat tagaggcagt tagaaaaaga
    29101 ccagggatgt acattggatc cactgacagc cgaggcttgc atcatttagt ctatgaaatc
    29161 gtcgataatg cagttgacga agcattatca ggttatggca gtgagattga tgtgaccatc
    29221 catgaagaca atagtataac tgttgcagat agcggacgtg ggatgcctgt aggaatgcat
    29281 gcttccggaa taccaacagt tgaagtgatt tttactgtgc tgcatgctgg cggaaagttt
    29341 ggtcaaggag ggtacaaaac ttctggtgga ctgcacggtg taggagccag tgtggtaaat
    29401 gccttatcga aatggctaat tgtaacgatc gttagagatg gcgtagagta tcagcagaaa
    29461 tttaaaaacg gcggtaaacc tgacggtact ttgaaaaaga taggaaaaac aaaaaaagca
    29521 aacgggacga ctgtccattt tttaccagat gatacaatat tcagtacaac gaaattttca
    29581 tatgaaattt tagccgaacg gttaagagaa tctgcctttc ttctaaaagg cgttaaaatc
    29641 tctctttctg atcttcgtgg agaagaacca gtcaaggaaa tttttcatta cgaagaagga
    29701 atcaaagaat ttgttgatta tctgaatgaa gaaaaagata cattgacacc agttgtttat
    29761 ttttcaggag aaaaagaagg tatcgaagta gaggtcgctt atcaatacaa tgacggttat
    29821 tcagaaaatg ttttatcttt tgtcaataac gttcggacaa aagatggggg aacccatgaa
    29881 gctggtatga aagcggcgat gacgaaatct tataatgaat atgcgagaaa agttggtttg
    29941 ctgaaagaac gggacaagaa tctggaaggt agtgatttca gagaagggtt agctgctgta
    30001 ctttctatcc gtgtacctga aaatcttctg caatttgaag ggcaaacaaa agaaaaactt
    30061 ggtacgccag ttgctcgtac cgtagtagat aacgtcatca gtgaacaaat gggcttttat
    30121 ttgcaggaaa atagcgaaat gagccagatg ttggtccgta aagcaatcaa agccagagaa
    30181 gcgagagaag cagcacgtaa agccagagaa gaaagccgaa atgggaagaa gcgtaaaaaa
    30241 ggcgaatctc ttctttcagg aaaattgaca cccgcacaat cccgaaaccc taagaaaaat
    30301 gaattgtatt tagtcgaagg ggattctgct ggcggatctg ctaaacaagg aagagacaga
    30361 aagttccaag caattctacc tttacgagga aaagtcatca ataccgaaaa agctaaaatg
    30421 caagatatct tgaaaaatga agaaatcaat acgatgatct atacgattgg tgcaggagtc
    30481 ggtcctgagt tttctatcga agactgtaat tacgacaaag taatcatcat gaccgatgcg
    30541 gatacagacg gcgcccatat tcaagtatta ctgcttactt tcttttatcg ttatatgaaa
    30601 ccgctgattg aagctgggaa agtatatatc gcgttaccgc ctttatataa agtatccaaa
    30661 ggacaaggaa agaagcaagt cattgaatat gcgtggacag atgacgaatt ggcagcaatg
    30721 attaaaaaag tcggcaaagg atatatgctg cagcgatata aaggacttgg agagatgaat
    30781 gcggaacaat tgtgggaaac aacgatggat ccgacttccc gtacgttgat ccgcgtccgt
    30841 atcgatgatg ctgcgcaagc tgaacgtcga gtaaccacac tgatgggtga caaagtagaa
    30901 cctcgaagaa aatggatcga aaatcacgtg caatttacgc tcgaagaaga tggcagtatc
    30961 ttagataaaa aagaggatac tgaaatctct ccttcagttt ctaatgattt attagatgaa
    31021 gaacgagcag ataaaaatga aaacaatcag ttatttgaag tagaatagga ggtcacctga
    31081 tggaaaatcg acaagaagta caagaattaa ctttagaaga agtgatgggt gaccgcttcg
    31141 gacgttattc caaatatatt atccaagaac gtgcactgcc tgatatccgt gatggattga
    31201 agccagtgca acgccggata ttatttgcca tgaacaaaga tggcaatacg tatgacaaag
    31261 gattccgtaa atcagcaaaa tctgtcggaa atatcatggg gaattatcat cctcatggtg
    31321 acatcagtat ttatgaagcg atggtccgca tgagccaaga ctggaagttg cgggaagtac
    31381 tgatcgaaat gcacgggaat aacgggagta tggacggtga tccacctgct gcgatgcgtt
    31441 atactgaagc cagattatcc aagttaagtg gagagatgct agcagatatc gagaaagaaa
    31501 cagtcgatct cgtgtggaat tttgatgata cagaaaaaga accaacagtc cttccagctc
    31561 gatacccaaa ccttctcgtc aatggatcga caggtatatc tgctggatat gcaacagaaa
    31621 tcccgactca taacttagct gaagtgatcg atggcacaat ctatatgatc gatcatccac
    31681 aagcgagcct tgaaaaattg atggaatata ttcctggacc tgactttcca acaggaggga
    31741 tcttgcaagg aaaagatgaa atcaaaaaag cttatgaaac aggacgagga aaagttattc
    31801 ttcgttcgaa aacaaagata gaggcaatga agggtaataa acagcaaatc gtgatcacag
    31861 aaatcccata tgaagtaaat aaagcgacct tggtgaaaaa aatggatgag atccgtttga
    31921 ataagaaaat cgatggaata gctgaagtac gagatgaaag tgatagaaca ggtctgcaaa
    31981 ttgtcgtcga attgaaaaaa gacgccaatg ctcaaggaat tttgaattat ttgttcaaaa
    32041 acactgagct acagatcaat tacaatttca atatggtcgc tatcgaccat atgactccgc
    32101 atcaagttgg actgaaagac attttaagaa gttatatcga gcatcgaaag caagtcataa
    32161 caaaacgtag tcaattcgat ttagccaaag ctcaaaaaag acagcatatc gtagaaggat
    32221 tgatgaaagc tttatcgatt ctggatgaag taatcgcaac gattcgtgaa agtaaagaca
    32281 aaaaagacgc caaaaagaat ttagtcgacg tctttcaatt tacagaagaa caagcagaag
    32341 caattgtcac attgcaatta taccgtttga cgaacacgga tatcacagag cttcaaaagg
    32401 aatccgaaag cttgattgcg caaatcacag agttgaataa aatcctttca aatgacaaag
    32461 agcttttctc tgttatgaaa aaagaattgc gagaagtgaa aaaaaattat tcttccgctc
    32521 gtctaacgac aatcgaagac gaaatcgaag aaatcaaaat tgatacgcaa gtcttagtgg
    32581 cacaagaaga cgtaattgtc agtgtgacgc gagaaggcta tgtgaagcga acaagccttc
    32641 gttcttatag tgcttccaaa ccagaagaaa tcgggatgcg agaaggcgac tacttgctat
    32701 actcaggtga attgagtaca ttggaccatg ttcttctgat cacaaataaa gcaaatgtga
    32761 tctaccgccc tgtccatgaa ctacccgatt tgaaatggaa agatgctggt gagcacattt
    32821 ctcaaacaat ctccaatctg tctgtagacg aatcgatttt agctgttttc ccatatcaaa
    32881 aaattcaagc agagaaaaca tttgtattca tcagcaagaa tggattgatc aagcaaacac
    32941 gaatgacaga gttcgaacca tggcgcacct ataagagccg tccgctcagc tgtatgaagc
    33001 taaaggcgga agaagatgaa ctgattgctg tgtatctgaa agaagatcag ccaggcttag
    33061 atgttttctt agtaacgcat caaggaatgg gtttgcgcta tcctttaaca gatgttcccg
    33121 ttgttggagc aaaggcagct ggagtgaagt ctatgaattt gaaagatgga gataggatta
    33181 tcaacgggct gcttgtgtta gcagaaggtg atacgccgat cgtaatcgtt actcagcgag
    33241 gagctgttaa acgcatgctt gctcaagaaa tcagccaaac ttcacgtgcg aagcggggag
    33301 tgacggtcat gcgtgagctg aaaaaaaatc cgcaccgtat tatctatatg tcggaaggaa
    33361 gatcaaaaga aataaccttg atgaatcaaa aagggcagca gttaacgatt gacccaactg
    33421 atttccctat aggtgatcga acatcgaatg gatcatttgc gatggatgag aaaaaaggtg
    33481 gagaagtcat cgaagttatc gatgctcctg agattttcat cgagtcgtaa ctgttgtagt
    33541 attttaatga agagacgcca tctgtattaa tacaggtggc gtctcttttt cattcatttt
    33601 taataaatcc ttatacgttt gcaaaaaaag atgaaagtgt tcacaatgct taaatgttgg
    33661 tatgtcaacg ttgatttaaa tatatgtaaa taaaacataa aaattgttaa ataaaaaaca
    33721 agaaaaccct tgcaaagata gggtgctgtg ttatactaat cacgtaaaaa ctgttacata
    33781 aaacagaaat ttaggaggtc catacaatgg agcaatggaa tggatttaaa ggtaccaaat
    33841 ggaaagaagg cgtagacact agagacttta ttcaaaataa ctacactgaa tatcgcggtg
    33901 atgacagctt tttggaaggc ccaacagaag caacagaaaa attatgggat gaattgcaaa
    33961 agttaaatga tatccaattc gagcgtaacg gtgtctatga tatggacaca gaaatcgtat
    34021 caactattac ttcacacgat gcaggctatt tggataaaga tttggaaaaa atcgtcggtc
    34081 tacagacaga caaacctttg aaacaagcat tcatgccgtt cggcggaatc aatatggcta
    34141 acaatgcgct aaaaagcaat ggctatacac caaacgatga attgacacat attttcactg
    34201 actggcgcaa aacacataat caaggtgtat ttgacgctta tactgctgaa atgcgtgctg
    34261 ctcgtaaaaa caaaatcatt acagggcttc cagacgctta cggtcgcgga cgtatcatcg
    34321 gtgactatcg tcgtgtggca ttgtatggaa tcgactattt gatggaacaa aagaaaaaag
    34381 atcatgatat gactggttat catacaatga gtgaagaagt gattcgttta cgtgaagaaa
    34441 tcactgaaca atatcgtgca ttagcgcaaa tgaaagaaat ggctgcgaaa tatggctatg
    34501 atatttctaa accagctgca aatgcaaaag aagctgtcca atggctatac ttcggatatt
    34561 tagcagcaat caaatcacaa aatggtgctg cgatgtcaat cggacgtatc tcagcattcc
    34621 ttgatatcta tatccaacgt gatttggata atggagtaat cacagaaaaa gaagcacaag
    34681 aattgatcga ccacttagta atgaaattac gtatggtcaa attcgcgcgt acacctgaat
    34741 ataaccaatt attctcagga ttccctatct gggctacttt atccattgca ggtatgggat
    34801 tagatggtcg ttcattagtt acaaagaatg atttccgtct gcttcataca ttaacaaata
    34861 tgggaccttc accagaacca aacttgactg ttttgtattc tgaacacctt ccagaaggat
    34921 tcagaacata tgctagcaga atcgctatcg aaagttcttc gattcaattc gaaaatgata
    34981 atcagcttcg tgaacaatgg ggttctgatg attgtgcgat tgcttgctgt gtatcaggaa
    35041 cagttgtcgg aaaagacatg caattctttg gcgctcgtgc taacttggct aaaacactat
    35101 tatatgccat caatggcgga gtcgatgaag tgactggcgc ccaagttgga ccagaatatc
    35161 gtccaatcac atcagaaata ctagactttg aagaagtgaa acataacttc atgaatatga
    35221 tggattggtt agcagaacta tatgtcaata cgttgaacat cattcattat atgcatgata
    35281 aatattctta tgaagcagct cagctggctt tgatggattc tgacttgcaa cgcacatttg
    35341 ctacaggtat tgctgggatc tctcatgcgg cagacagctt gtcagcgatc aaatatgcaa
    35401 aagtaaaagc aatccgtaat gaaaaaggaa tagcagttga ttttgaagtg gatggcgact
    35461 tccctaaata cggaaatgat gatgaccgtg cagatgaatt agtttcatgg atcttggaat
    35521 acttcatggg acaaatcaaa cgtcataaaa cttaccgcaa ctcaaaacca acaacttcat
    35581 tattgacgat tacttcaaat gttgtttacg gtaaaaatac aggaaatact cctgatggcc
    35641 gtgaagctgg aaaaccactt gcgccaggag caaatccaag ttacaatgcg gaacaaagtg
    35701 ggttattagc tagcctgaac tcaacagcta aaatgaatta cggctgtgca ttggatggaa
    35761 tttcaaatac ccaaacaatc aatccttcag gtcttggaaa agaacaagat acaagaatcg
    35821 aaaacttgcg taatgtctta gatggatact tctcaaaagg agcttatcat ttgaatgtca
    35881 atgtattcag taatgattta ttgcgtgatg caatggaaaa tccaatgaaa tatccgaact
    35941 tgacgatccg ggtatctggt tatgcagtta aattccgtga cttgacaaga gaacaacaat
    36001 tagatgtctt gatgagaact tcacacgaca gaatgtaaaa agttcgtgaa aaaagttttt
    36061 cgacttagcg tcgaaaggac gttgaatagc catcgcatca aaatcgaata atcagtatgc
    36121 ttatttgaaa gaaacgattt acaggggcag gacttgtatg ttccgtccct gtaaatttta
    36181 cacataacga agcggcaatt gcgaaaaatt ttgttcagaa agcttcgctt cgacagctaa
    36241 cttgtactta aaatgaaatc aaagaaaaga tgtgaactta aatggaagaa aaaacaatcg
    36301 gctatgttca ttctatcgaa actttcggtt cagtagacgg acctggactg cgctttgtcg
    36361 tgtttatgca aggatgccgc atgcgttgcc aattttgtca caatccagat acttggaata
    36421 ttggcggagg gaaagaatac acagcagatg aacttttaga taaagcagaa cgcttcagac
    36481 cttactgggg agataaaggt ggtattactg ttagtggtgg agaacctcta ttgcagatcg
    36541 attttttgat cgaactattt aaaaaagcaa aagagcggaa gatgcatact acattggata
    36601 catgcggaaa accttttacg tatgaagaac ctttcttttc acgttttcaa gaattgatga
    36661 agtatacaga tttactgtta tttgacatca aacacatcga taatgaagag cataaaaaac
    36721 taacgcattg ggacaatgaa aatattttag aaatggctca atatttgtca aaaatcaaca
    36781 aaccggtttg gattcgccat gtactagttc ctgagcgaag tgattatgat gaatatttga
    36841 tccgactaga caactttatt caaaccttgt caaatgtaga cagagtagag attcttcctt
    36901 atcatacgat gggaaaatac aagtgggaaa cacttggttt gaaatatcca ttagaaggga
    36961 tcgaaccacc tacaaaagaa cgtgtcgaaa atgctaaacg attgcttcat acagaagagt
    37021 atacaaaata tttaacgcga taggtcgcat cggaaagaag ataagtattg cttctttccg
    37081 tttttttgct attattaaat aggagaaatc acaaaggagc gaatgacatg tcaaaaattt
    37141 tagtttttgg tcaccaaaat ccagatacag atgcaatcgg ggcagccatt gcgttttcac
    37201 acttgcaaaa tgaattaggt aaagaaacag aagcagttgc tctaggtaca cctaatgaag
    37261 aaacacaata cgcattagac cactttcagc tatctgcacc acgagtagta gagtcagcta
    37321 aagcagaaaa tgcagatgtc atgcttgtgg atcataatga attccaacaa agtatttcag
    37381 atatcagcga agtaaatatt ttagctgtag tagaccacca tcgtattgca aactttgaaa
    37441 cagctaatcc attgtactat cgtgcagaac cagttggatg tacaagtacg attattttaa
    37501 aactatataa agaaaaccaa gtaacaattc ctaaagaaat cgcaggaatc atgctctcag
    37561 ctatcatttc agatacattg ttgttcaaat caccaacatg tacgcctgaa gatgaagcag
    37621 ctgcaaaaga acttgcagca atcgctgaag tcgatctaaa cacttatgga cttgaaatgt
    37681 tgaaagcagg aacaaatcta agtgataaat cagctgcggt tcttcttgat ttggatgcaa
    37741 aaagtttccc aatgggcagt tccaacgttc gtatcgctca agtcaataca gtggacctta
    37801 atgaagtgat ggatcgacaa gcagagctag aagcagcaat ggaagatgaa atgaaagcaa
    37861 atggctacga tctgttcgta ttagttgtca caaacatctt agacagtgat tcagaattac
    37921 ttgttgctgg taaaggaaaa gaaaaagtag aagaagcttt ccaaacaact ttgaccaaca
    37981 accgtgcttt gcttaaaggt gtcgtttcac gtaaaaaaca agtcgtacct caattgacgg
    38041 ctgtatttag ctaatcaaat ggtagtatga agcattcaaa atgaatggat aagcgattga
    38101 tataaaaaga aacatagaac atactaaagg ccgaagaaat ggaggagaat aacagtcaat
    38161 tatgactctt gttcttcctc ctttttacgt aaaagccttt taagtaaaat aaaacggata
    38221 gaaaagagaa catatatgaa ctattattca aaaaataagg aaaactttta tcaaaaactg
    38281 accgatgatc cgcttttttc ttcattgacc gattatctat atgaacatcg agaacaagaa
    38341 acgattctaa gagaattgaa aaaagaattt tcgcaaaata aattttccca ttttcttgat
    38401 ttattgatcg atgcaggatt aatcaagagg gaagaacgaa gatatcattt gaactttcca
    38461 atatttgatc ctaaagatta cttgcaacaa gccacttcgg ctgctgaaac catagctgaa
    38521 caattgaaga gactctctgt tgatgaacaa aaattagcga tgggagaagt tatttgggcg
    38581 tattgtttcg aagatgaacg gaaagaagcg tatttttatg gagtaagaaa cagccgagag
    38641 acagaattgc tgaggacaac tgcaggaaat gagaagtacc gttttatcac actttcctct
    38701 atagaacact tcccgcttac tttggctaat tacttttttg tacagaagaa tcagcttcct
    38761 gtaacgaagg cattcaaaga attagccgaa cttattggag acgtaaatga atcgtacttt
    38821 tttgatcaaa tagaagttat cgttgacagg atacgtaaga ataaatataa aaatcgccgg
    38881 cctagtattt tccatcagtc tctacttgtg acgaatacta taaaggaaga agaatcattc
    38941 acattagaat tacctattgt cgaaaaaaac aattttgaga ttgaattacc aacattggat
    39001 ccttcattga caatggagga aatagccttt cttaaacgac aaatcttttc tgagctgagc
    39061 aagaaattta taccgcatgc attttcttat atcaaagaat atggaacagt tttagtgagt
    39121 aaaacctaag agagcagtaa gcaaaaatta tactgactct tgcaggagga gcctttctct
    39181 tcctttatgg ttaaaagaat tatttgagta ttttgcctat ctaccaccta ttagattcac
    39241 tacatggtct ggaaacaaag gaatgcaaaa aaatggtgag aaatgtggaa tgacaaagga
    39301 agtagtgata agaaaagtta gatttttaaa taatcaatat tatgattcag tcaagtatgg
    39361 cattttatgg gaagaactag ctgattagat taggaagtcc atttgataat ccagaatcac
    39421 taagaaacga atggcgagtc attaataatt tatccaatct tgggttattc ttttagattg
    39481 aagtgtttaa aactaaaaaa gcatttatga agtcgtaata gtcaggcttc aaaatgcttt
    39541 ttattttgtt tgttttttct ttgccacata aatacaccta gactgacgat gaagagcata
    39601 tccaacagat ttcctgcgac ttggatcgtc gaagggttgg tcagtggcat taaagcaagg
    39661 acaaaaaagg aaaacaagct gtaaataaaa caggtgacag gaactaatcg aaagaagagc
    39721 ggacatttca attggctaaa aatgagtgaa aaaataaaat aagtaaaaat ggataaaatc
    39781 acgactaaac cattcatgat aaagaagaaa agcgtattgt gattaaacga ataaaaaaga
    39841 attgctaaaa aagaaaacgt tttaaatgca atcaatacgc ttgaagcttc gtttaacgta
    39901 ccaggatgtc gatgaaaaaa ataggcaccg aaataaacag aaaacagtaa gccgcaaaga
    39961 gaagacaaaa tcaaaaaaaa cggattttct tcggcttctg tgatatcaga atgatccaaa
    40021 agcaaccagc ctaatgcact agaaggcgca tgtgaggtct taaggtatga ataacataga
    40081 aaaaataaga tcgtcaaagt tgcaaaaaaa gagcctaaat gtaaaaaatt agtggttcta
    40141 cgttgaattt ccataatatc tccaaattaa attgataagt atataaagta taataacaaa
    40201 ttgatgagta tatgtcgatg ttttttaatc aaaataataa ataaaaactg tctaacattt
    40261 aaaatttaat ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt
    40321 ctcacttttt tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa
    40381 gcacgctctt cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa
    40441 agtatttcga gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca
    40501 taaccgtgtt ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa
    40561 atcgttgtga ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg
    40621 atacctactg cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct
    40681 aaaacaaggg ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca
    40741 atcagtcgat agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca
    40801 aaattaatga ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc
    40861 atttatataa caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca
    40921 tacaaaaaaa tgataaaatg attataatag aattataaag ataggttagg tgattaaaat
    40981 gaaaacggtc aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata
    41041 cttaggtgtc tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc
    41101 gctgacagat ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat
    41161 tttcggtagc gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt
    41221 aggagtggtg ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat
    41281 attatttggt acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc
    41341 aggaaattta atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt
    41401 ccatcatatt ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt
    41461 tgtcatctat tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa
    41521 aattgcgcag cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa
    41581 acagccggtt tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt
    41641 tgaatttgac acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc
    41701 agttggcggg gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca
    41761 tatagtagcg ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat
    41821 cgtaaggcgc gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc
    41881 atgggttaag agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt
    41941 cttttggcat ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga
    42001 aaaaaatcgg cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg
    42061 acatatggta caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat
    42121 aaaaaagtat aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt
    42181 ttattttggg ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga
    42241 caacatgtta gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat
    42301 gtttcaaaaa tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg
    42361 aaagagttag ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa
    42421 gcacttgtca cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca
    42481 actgagccct attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat
    42541 tattctctcc aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc
    42601 atcacaggtg tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc
    42661 ttgtttgggg aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca
    42721 gcaaaagcaa cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc
    42781 gaagtggacg agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa
    42841 gttgatcttc ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat
    42901 atgacagaaa actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg
    42961 ctagcactag gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt
    43021 ggtgtgattg gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg
    43081 atcaagcaag atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata
    43141 gaagaaaaag gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag
    43201 acaacgaatt aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa
    43261 aataatcagc gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca
    43321 tttttgagaa ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg
    43381 tacgatgatg agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga
    43441 caagaagatc ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat
    43501 ttcacgaaca cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag
    43561 tcagctccgc gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc
    43621 cattaccaat cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt
    43681 tctgtcagac aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt
    43741 gacacgaagg tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc
    43801 ccagacccta gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg
    43861 gatgactctt atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt
    43921 gagccgtctg tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa
    43981 gaaaaaaaat cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat
    44041 gatcaaggaa actctttggg agaaaatagc tattttaaat aagtgatata caaatttata
    44101 ttcggctaca gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa
    44161 gtttatatcg tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa
    44221 gaaaataatg gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt
    44281 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
    44341 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
    44401 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
    44461 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
    44521 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
    44581 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
    44641 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
    44701 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
    44761 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
    44821 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
    44881 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
    44941 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
    45001 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
    45061 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
    45121 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
    45181 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
    45241 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
    45301 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
    45361 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
    45421 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
    45481 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
    45541 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
    45601 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
    45661 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
    45721 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
    45781 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
    45841 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
    45901 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
    45961 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
    46021 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
    46081 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
    46141 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
    46201 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
    46261 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
    46321 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
    46381 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
    46441 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
    46501 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
    46561 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
    46621 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
    46681 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
    46741 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
    46801 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
    46861 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
    46921 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
    46981 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
    47041 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
    47101 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
    47161 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
    47221 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
    47281 taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc aagtcaatgt
    47341 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
    47401 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
    47461 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
    47521 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
    47581 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
    47641 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
    47701 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
    47761 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
    47821 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
    47881 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
    47941 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
    48001 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
    48061 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
    48121 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
    48181 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
    48241 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
    48301 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
    48361 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
    48421 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
    48481 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
    48541 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
    48601 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
    48661 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
    48721 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
    48781 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
    48841 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
    48901 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
    48961 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
    49021 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
    49081 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
    49141 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
    49201 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
    49261 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
    49321 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
    49381 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
    49441 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
    49501 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
    49561 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
    49621 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
    49681 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
    49741 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
    49801 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
    49861 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
    49921 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
    49981 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
    50041 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
    50101 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
    50161 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
    50221 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
    50281 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
    50341 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
    50401 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
    50461 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
    50521 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
    50581 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
    50641 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
    50701 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
    50761 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
    50821 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
    50881 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
    50941 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
    51001 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
    51061 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
    51121 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
    51181 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
    51241 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
    51301 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
    51361 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
    51421 aaaaaaggct atcaggaagc tattaaggat ccaaatgata gtgctaaact gttgcaaaaa
    51481 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
    51541 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
    51601 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
    51661 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
    51721 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
    51781 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
    51841 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
    51901 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
    51961 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
    52021 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
    52081 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
    52141 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
    52201 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
    52261 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
    52321 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
    52381 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
    52441 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
    52501 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
    52561 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
    52621 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
    52681 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
    52741 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
    52801 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
    52861 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
    52921 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
    52981 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
    53041 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
    53101 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
    53161 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
    53221 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
    53281 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
    53341 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
    53401 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
    53461 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
    53521 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
    53581 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    53641 ta
//
LOCUS       DAZUKT010000009        52709 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000009 DAZUKT010000000
VERSION     DAZUKT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.009"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            271..1053
                     /gene="rpsB"
                     /locus_tag="ACG816_000537"
     CDS             271..1053
                     /gene="rpsB"
                     /locus_tag="ACG816_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4919453.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1152..2033
                     /gene="tsf"
                     /locus_tag="ACG816_000538"
     CDS             1152..2033
                     /gene="tsf"
                     /locus_tag="ACG816_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4919454.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2169..2891
                     /gene="pyrH"
                     /locus_tag="ACG816_000539"
     CDS             2169..2891
                     /gene="pyrH"
                     /locus_tag="ACG816_000539"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4919455.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2894..3451
                     /gene="frr"
                     /locus_tag="ACG816_000540"
     CDS             2894..3451
                     /gene="frr"
                     /locus_tag="ACG816_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4919456.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            3646..4458
                     /locus_tag="ACG816_000541"
     CDS             3646..4458
                     /locus_tag="ACG816_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4919457.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            4455..5255
                     /locus_tag="ACG816_000542"
     CDS             4455..5255
                     /locus_tag="ACG816_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4919458.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            5416..6684
                     /gene="rseP"
                     /locus_tag="ACG816_000543"
     CDS             5416..6684
                     /gene="rseP"
                     /locus_tag="ACG816_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4919459.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            6752..8461
                     /locus_tag="ACG816_000544"
     CDS             6752..8461
                     /locus_tag="ACG816_000544"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4919460.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            8673..13031
                     /locus_tag="ACG816_000545"
     CDS             8673..13031
                     /locus_tag="ACG816_000545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4919461.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            13176..13649
                     /gene="rimP"
                     /locus_tag="ACG816_000546"
     CDS             13176..13649
                     /gene="rimP"
                     /locus_tag="ACG816_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4919462.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            13672..14847
                     /gene="nusA"
                     /locus_tag="ACG816_000547"
     CDS             13672..14847
                     /gene="nusA"
                     /locus_tag="ACG816_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4919463.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            14869..15162
                     /locus_tag="ACG816_000548"
     CDS             14869..15162
                     /locus_tag="ACG816_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4919464.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            15159..15470
                     /locus_tag="ACG816_000549"
     CDS             15159..15470
                     /locus_tag="ACG816_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4919465.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            15483..17789
                     /gene="infB"
                     /locus_tag="ACG816_000550"
     CDS             15483..17789
                     /gene="infB"
                     /locus_tag="ACG816_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4919466.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            17816..18163
                     /gene="rbfA"
                     /locus_tag="ACG816_000551"
     CDS             17816..18163
                     /gene="rbfA"
                     /locus_tag="ACG816_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4919467.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(18320..18776)
                     /locus_tag="ACG816_000552"
                     /pseudo
     CDS             complement(18320..18776)
                     /locus_tag="ACG816_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            18941..21303
                     /locus_tag="ACG816_000553"
                     /pseudo
     CDS             18941..21303
                     /locus_tag="ACG816_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            21671..22594
                     /gene="truB"
                     /locus_tag="ACG816_000554"
     CDS             21671..22594
                     /gene="truB"
                     /locus_tag="ACG816_000554"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4919468.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            22598..23539
                     /gene="ribF"
                     /locus_tag="ACG816_000555"
     CDS             22598..23539
                     /gene="ribF"
                     /locus_tag="ACG816_000555"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4919469.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            23891..24676
                     /locus_tag="ACG816_000556"
     CDS             23891..24676
                     /locus_tag="ACG816_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4919470.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            24689..25540
                     /locus_tag="ACG816_000557"
     CDS             24689..25540
                     /locus_tag="ACG816_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4919471.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(25664..26116)
                     /locus_tag="ACG816_000558"
     CDS             complement(25664..26116)
                     /locus_tag="ACG816_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919472.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(26581..26949)
                     /locus_tag="ACG816_000559"
     CDS             complement(26581..26949)
                     /locus_tag="ACG816_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4919473.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            27099..27581
                     /locus_tag="ACG816_000560"
     CDS             27099..27581
                     /locus_tag="ACG816_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4919474.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            27618..28628
                     /locus_tag="ACG816_000561"
     CDS             27618..28628
                     /locus_tag="ACG816_000561"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4919475.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(28743..29456)
                     /locus_tag="ACG816_000562"
     CDS             complement(28743..29456)
                     /locus_tag="ACG816_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919476.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(29629..30236)
                     /locus_tag="ACG816_000563"
                     /pseudo
     CDS             complement(29629..30236)
                     /locus_tag="ACG816_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            30720..30947
                     /locus_tag="ACG816_000564"
     CDS             30720..30947
                     /locus_tag="ACG816_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4919477.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30963..32129
                     /gene="hemW"
                     /locus_tag="ACG816_000565"
     CDS             30963..32129
                     /gene="hemW"
                     /locus_tag="ACG816_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4919478.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            32363..33406
                     /gene="hrcA"
                     /locus_tag="ACG816_000566"
     CDS             32363..33406
                     /gene="hrcA"
                     /locus_tag="ACG816_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4919479.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            33500..34063
                     /gene="grpE"
                     /locus_tag="ACG816_000567"
     CDS             33500..34063
                     /gene="grpE"
                     /locus_tag="ACG816_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4919480.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            34114..35943
                     /gene="dnaK"
                     /locus_tag="ACG816_000568"
     CDS             34114..35943
                     /gene="dnaK"
                     /locus_tag="ACG816_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4919481.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            36094..37260
                     /gene="dnaJ"
                     /locus_tag="ACG816_000569"
     CDS             36094..37260
                     /gene="dnaJ"
                     /locus_tag="ACG816_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4919482.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(37342..37971)
                     /locus_tag="ACG816_000570"
     CDS             complement(37342..37971)
                     /locus_tag="ACG816_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919483.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            37983..38156
                     /locus_tag="ACG816_000571"
     CDS             37983..38156
                     /locus_tag="ACG816_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919484.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(38201..38938)
                     /locus_tag="ACG816_000572"
     CDS             complement(38201..38938)
                     /locus_tag="ACG816_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4919485.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(38940..40442)
                     /locus_tag="ACG816_000573"
     CDS             complement(38940..40442)
                     /locus_tag="ACG816_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4919486.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            40701..42695
                     /gene="uvrB"
                     /locus_tag="ACG816_000574"
     CDS             40701..42695
                     /gene="uvrB"
                     /locus_tag="ACG816_000574"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4919487.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            42705..45521
                     /gene="uvrA"
                     /locus_tag="ACG816_000575"
     CDS             42705..45521
                     /gene="uvrA"
                     /locus_tag="ACG816_000575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4919488.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            45784..47049
                     /locus_tag="ACG816_000576"
     CDS             45784..47049
                     /locus_tag="ACG816_000576"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4919489.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            47053..47784
                     /locus_tag="ACG816_000577"
     CDS             47053..47784
                     /locus_tag="ACG816_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4919490.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            47919..48803
                     /gene="rapZ"
                     /locus_tag="ACG816_000578"
     CDS             47919..48803
                     /gene="rapZ"
                     /locus_tag="ACG816_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4919491.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            48800..49798
                     /gene="yvcK"
                     /locus_tag="ACG816_000579"
     CDS             48800..49798
                     /gene="yvcK"
                     /locus_tag="ACG816_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4919492.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            49832..50767
                     /gene="whiA"
                     /locus_tag="ACG816_000580"
     CDS             49832..50767
                     /gene="whiA"
                     /locus_tag="ACG816_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4919493.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            51310..51960
                     /locus_tag="ACG816_000581"
     CDS             51310..51960
                     /locus_tag="ACG816_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919494.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            52147..>52709
                     /locus_tag="ACG816_000582"
     CDS             52147..>52709
                     /locus_tag="ACG816_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFS4919495.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        17756 a         9029 c        11353 g        14571 t
ORIGIN      
        1 tattttatgg aaaaaggata ataaagaaac aaagagaaat ctactgtttt tcttgaattc
       61 tttttatctt tcaggtaaac tattgaatgt tgagcagaaa agtatatttg ctcttgcgat
      121 acggatcttt tgtctgtata atagctgagg gtgctttatg catcagcaaa tccacattct
      181 tattggattg tcggtaggtg cttgaaaaag tttgccggca aggtagaaga taagaagagg
      241 aaaaataaaa ccaaatcgga ggaaacaaac atggcagtaa tttctatgaa acaattacta
      301 gaagccggcg tacactttgg tcaccaaact cgtcgctgga atccaaaaat gaagaaatat
      361 atcttcacag aaagaaacgg aatctacatc attgacttgc aaaaaacagt taaattagta
      421 gatgcagctt atgattacat gaagagcgtt gctgaagaag gcggcgtagc attattcgta
      481 ggtacaaaaa aacaagcaca agaagcaatc aaagaagaag caactcgtgc aggacaatac
      541 ttcgttaacc atcgttggtt aggcggaaca ttgacaaact gggatactat ccaaaaacgt
      601 atcagccgtt tgaaacaaat caatgcaatg gaagaagatg gaacttttga agttctacct
      661 aaaaaagaag ttgctggttt gaacaaagaa cgtgaacgtc ttgaaaaatt cttaggcggt
      721 atcgctgata tgccaagaat cccagatgta atgtacatcg ttgacccacg taaagaacgt
      781 attgctgttc aagaagcaca caaattaaat atcccaatcg tagctatggt tgatacaaac
      841 tgcgatccag atgaaatcga cgtagtaatc ccttcaaacg acgatgcaat tcgtgccgtt
      901 aaattgatta cttcaaaaat ggctgatgct ttcatcgaag gaaaccaagg tgaagatcaa
      961 gtagttgaag aagatttcac agaagaaaac acagcaacat caatcgaaga aatcgtagat
     1021 gttgttgaag gcgacaattc ttcagcagaa taattttgaa tgatggagct gtttcaaagg
     1081 ctgggcgaca agcctcactc tcctttgaag cagctttttt taaaaagaaa aacacacagg
     1141 aggagttaaa catggcagac gttacagcta aaatggtaaa agaactacgc gaaatgactg
     1201 gcgtaggaat gatggatgcg aaaaaagcat tagttgaagt agaaggcgac atggaaaaag
     1261 cagttgatct tttacgtgaa aaaggaatgg caaaggctgc gaagaaaaat gaccgcattg
     1321 cagctgaagg attggcttca gttgctgtga aaggtaacac tgcagcaatc gttgaagtaa
     1381 actcagaaac agactttgtt tctaaaaacg aaatgttcca agatttagta aaagaaattg
     1441 cagaacttgt tgctgaaaac aaaccagcag acatggaagc tgcaatgaaa atcaaaacag
     1501 ataaaggtac aattgaatct gacttgatcg aagcaactca agttatcgga gaaaaaatca
     1561 gcttccgtcg ctttgaagtt gtagaaaaag acgacaatgc tgcatttggt ggatacttgc
     1621 atatgggcgg acgtattgcc gtattgactg ttctagatgg aacaactgac gaaacagttg
     1681 caagagacgt agctatgcac gtagcagcta tcaatcctcg ttatgtaaac gaatcacaaa
     1741 ttccagaagc tgaattagaa catgaaaaaa ctgttcttac agaacaagca ttgaacgaag
     1801 gcaaaccagc gaatatcgtt gaaaaaatgg tagaaggacg cttgaagaaa ttcaaagcag
     1861 aaatcgcttt ggtggaccaa ccattcgtta aagatcctga tatgactgtt gaaaaatacg
     1921 ttgcatcaaa aggcgcaact gttaaaacat tcgtacgttt cgaagttggc gaaggtatcg
     1981 aaaaacgcga agataacttt gtagaagaag ttatgagcca agtgaaaaaa taattctgtt
     2041 gaataacaga cgggaacgca cggtttgtgc gctccctttt tttaagcaaa acaagaaaaa
     2101 ccagtcttag agtgtcgaaa tcatctaagt atggtaaaat ggcaatagaa aatattggag
     2161 gaatgaacat ggtcacaccg aaatatcagc gtgtagtctt aaaattgagt ggagaagctt
     2221 tagctgggga cgcaggattt ggaatcaaac caccagtaat caaagaaatc atccaagaaa
     2281 tcaaagaagt tcacgagctg ggcgttgaaa tggcaatcgt tgtgggcggc ggaaatatct
     2341 ggcgcggaca aatcggagca caaatgggca tggaacgtgc tcaagctgat tacatgggta
     2401 tgttagctac agttatgaat gccttagctt tacaggatac gttggaaaac gtgggtgtac
     2461 cgacacgcgt gcaaacttcg attgaaatgc gtcaaatcgc agagccatat attcgccgta
     2521 aagcagaacg tcatttagaa aaaggacgga tcgttatctt tgcaggcggt accggcaacc
     2581 cgtacttctc gacagacaca acagcagcat tacgcgcggc agaaatcggc gcagatgtta
     2641 tcctgatggc taaaaacaat gtagacggtg tttattctgc tgatccgaaa gtagatgcaa
     2701 atgcagtaaa atttgaagaa ctgactcatc tggaagttat tgccaaagga ttacaagtca
     2761 tggattcaac tgcaagttca ctaagcatgg ataatgatat tccactgctt gtcttcaatt
     2821 taaatgaaca cggaaatatt cgccgtgcaa tcttaggcga aaatatcgga acaactgtaa
     2881 gggggaaata aaaatggcag atgcaattat gacagaagca aaagaaaaaa tgaaaaaagc
     2941 agcacaaaat cttcaacgtg aacttgggca aatccgcgca ggccgtgcga acgctagcct
     3001 tttggaccgt attactgtga actactacgg tgcgccaaca ccattgaacc aaatggcatc
     3061 aatccaaatt ccagaagcac gtgttttaat gatcacacct tttgacaaga gcattcttca
     3121 agatgttgaa aaagctatta tggcaagtga tatcggaatc agtccaacca atgatggaaa
     3181 tgtcattcgc ttagttatcc cgcaactgac agaagaacgc agaaaagaat tagctaaaga
     3241 tgttaaaaaa gaagcagaaa atgcaaaaat cgcgattcgt aacatccgtc gtgatgcgat
     3301 cgatgaatat aaaaaacaac aaaaaaatgg tgatattacc gaagatgatt tgcgcggatt
     3361 agaaaaagac gtacaaacat tgacagataa cagcatcaaa gaagtagata agatcgctgc
     3421 tgataaagaa aaagaattat tggaagttta ataaaatcat aaaaacagag gggcggatca
     3481 ttccgcttcc tctgttttta tttatatttc ttttatttaa aaattaaaaa aagaaaaaat
     3541 tattcagttt ttaaataaaa aaacaatgat ttcataggaa aaacagttag atattgctat
     3601 aatacttaag atagtgggaa tcttttaagt ataggaggaa aaagaatgtt acgttttttt
     3661 ccgcaaaaaa acaaatatat tcaagaaaaa agcgaatttc aatttaatac cgatggacca
     3721 attcctcagc acattgccat catcatggac ggcaatggtc gttgggcgca aaaccgtcgt
     3781 ttgccacgag ttgctggcca taaagaaggc atggaaacag taaagaaagt cacaaaaaaa
     3841 gcatcaaggc ttggagtgaa agtgttgacg ctttatgcct tttcgactga aaactggaag
     3901 cgaccaaaag atgaagtaag ttttctcatg caactacctg tcgacttctt tgatacgttc
     3961 gtaccagaat tgattaaaga aaatgtcaaa gttcatgtca tgggttatga aaacgtattg
     4021 ccagaacata cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa caatactgga
     4081 atggtattga attttgcttt gaattatgga agcagagcag agatcgtgac tgctgtccaa
     4141 gaaattgccg aagaagtagc aaaaggagaa atacacgcag aagaaatcga tgatgaattg
     4201 attgctaagc atttaatgac aggtttcttg cctaaagagt tacaagatcc tgagctgatg
     4261 atccgtacaa gtggagaaga gcgaattagt aattttctgt tatggcagat cgcatatagt
     4321 gagctgtatt ttacaaaagc gctttggcct gattttgatg gtgcgcatct tgaagaagca
     4381 atcgcctctt atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac agaagatgag
     4441 aaaggggatc agtcatgaaa caacgtgtgg tcacagcagc cgtggcgcta gtcttgttta
     4501 ttccgattat atggatcgga ggcatagcgg ttgagcttac agcagcaatc ttagcagtaa
     4561 taggagttta cgagctgttt cggatgaaag gattgacact cttaagtttt gaaggagttt
     4621 tatccgccgt tggtgcagtg ttacttgttt tgccaaaaga aagatggttt ttctttcttc
     4681 cagagaatgt aagtacattt atgttatttt atcttatagt tatgattctt ttaggaacat
     4741 cagttatttc taaaaatacg tatacgattg atgaagcagg ttttcctgtg cttgtcagct
     4801 tgtatgtggg aataggtttc cagaattttg tcaatgcacg aacagaaagc ttggctgttt
     4861 tgatatttgg tctatttatt gtttgggcaa ctgatatcgg agcatatatg gttggtcgac
     4921 gttatggtca gcataagtta agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc
     4981 tcggtggtat tggtagtgct gttgtagtcg catttcttta tttattgcta tacccagcaa
     5041 aagaactatt taatcatggt atgtttgtca tgcttctgat gacgattatt ttttcagcag
     5101 tcggacaatt tggtgatttg gtcgaatctg caatcaaacg tcattatgat gtgaaagact
     5161 cgggaaatat cttaccaggc catggtggaa tcttagaccg tttcgacagt ttgctgtttg
     5221 tatttcctat catgcattta tttggcatta tttagtaagt atacctgcgt ttctttgtga
     5281 atttcataaa agacgcaggt ttttattttt cattatattg atactattgg tgtctttggt
     5341 atactatggt aaaattatag tgttttatcg ttgttttata aaaaacacaa aaaacaagta
     5401 aaggagaaaa aagctatgaa aacgattctg acatttatca tcgtttttgg tatattagtg
     5461 attgttcatg agtttggtca tttcttcttt gcgaaacgat caggaatcct cgttcgcgaa
     5521 tttgctatcg gtatgggtcc gaaaatttat gggcatcaag caaaagacgg gacaacatat
     5581 acgcttcgcc tattacctat tggcggttat gttcgaatgg caggcaatgg ggatgatgaa
     5641 acagaaatgg caccaggaat gcctctttct cttttactaa attctgacgg catcgtagag
     5701 aagataaatt taagtaagaa aatccaattg acaaatgcta tcccgatgga attgagccgt
     5761 tatgatttag aagatgagct gactatcact ggttatgtga atggagacga aacagaagta
     5821 gtaacctacc ctgttgatca cgatgcgacg attattgaaa acgacggaac agaaatcaga
     5881 attgcgccaa aagacgtaca gttccaatcg gctaaattgt ggcagcgtat gctgaccaac
     5941 tttgctggac ccatgaataa ctttattttg gcaatcctgt tattcatcat tctggcattt
     6001 atgcaaggtg gtgttcaagt gacgaacact aatcgcgtag gagagatcat gcctaatgga
     6061 gctgcggcag aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga cggaaaagaa
     6121 atccattcat ggaatgatct gacaacagtc attacgaaaa atccggataa aacattagac
     6181 tttaagatcg aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa atctgtagag
     6241 tcaaatggcg aaaaagttgg ccaacttggt ataaaagccc caatgaatac aggttttatg
     6301 gataagatca ttggagggac gcgtcaagct ttcagcggtt cattggaaat tttcaaagcc
     6361 ttaggatcac tatttacagg tttcagttta gataaattag gcggacctgt catgatgtac
     6421 caattgtctt cagaagcagc aaatcaaggt gtaacaactg ttattagttt gatggcgctt
     6481 ctttcaatga atctcggaat cgtcaatctg cttccgattc ctgccttaga tggcgggaaa
     6541 ttagtcttga atatttttga agggattcgc gggaaaccat taagtcaaga aaaagaagga
     6601 atattgacat tagctggctt tggattttta atgttgttga tggtgctagt tacatggaac
     6661 gatattcaac gattcttttt ctagaaaaag ttgacaaata gggtataata acaaacgata
     6721 actattttga ggaaataaag gagtatatag aatgagacaa tcaaaaatgt tgattccaac
     6781 attacgcgaa gttccaaatg atgcagaagt attaagccat caaatcttgc taagagctgg
     6841 atatatccgc caagtatcag cgggtattta ttcatatctg ccattagcaa atcgtgtatt
     6901 ggaaaaactg aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg tagaaatgct
     6961 gatgcctgcc atcttgccag cagaactttg ggaagaatcc gggcgttacg aaacatatgg
     7021 accaaacttg tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg gaccaactca
     7081 cgaagaaact tttactgatt tgatgcgtaa tgaaatcaat tcatataaga aattaccttt
     7141 aaatctttat caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct tcggtttgct
     7201 tcgcggaaga gagtttatca tgaaagatgc gtattctttc catgcaacgg aagaaagttt
     7261 ggacgagaca tataaagatt acgaaaaagc ctacaccgaa gtattcaagc gttgtggctt
     7321 agaatttcgc tcgattatcg gagacggtgg cgcaatgggc gggaaagact caaaagaatt
     7381 catggcgatc tcagaaatcg gggaggatac gatctgttat tcgactgaaa gtgattatgc
     7441 tgcaaacttg gagatggcta caagctacta tgtatcaaaa aaatcgcatg aaacacagct
     7501 tgaattggag aaagtagcta cacctaacgt aaaatcaatt gacgaagtag cagatttctt
     7561 ttctgttgaa cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg aacaacctgt
     7621 ccttgttttg gtacgcggag accatgaagt caatgacgta aaactgaaaa actttttatc
     7681 tgctgacttt ttagaagaag caacagaaga agaagcacaa aaatatctag gtgctgagtt
     7741 tggttctgtc ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag accgttatgt
     7801 ccaagattta gcgaatgcca ttactggagc gaacgaaaca ggattccact ataaaaatgt
     7861 gaatccagaa cgcgatttca ctgtactttc ttatgaagat cttcgttttg tacaagaagg
     7921 agatccttca ccagacaaca atggtgttct tgcctttact cgaggaatcg aaatcggaca
     7981 tatcttcaaa atcggcactc gttacagtga atctatggga gcaactgtac tagacgaaaa
     8041 tggacgagaa aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc gtttattatc
     8101 agcaatcgta gaacaacatt cagatgaaca aggaatcaac tggccaaaag gaattgcgcc
     8161 atttgatttg catatcgtcc aaatgaatct aaaagacgaa taccaaacaa acctgacgaa
     8221 tgaactagaa gaagcgatga caaaagctgg ctatgaagta ctggttgatg acagaaatga
     8281 acgcgcagga gtgaaattcg cggattctga tttgattggc tgtccaatca gaatcactgt
     8341 cggtaaaaaa gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg gcgaaatggt
     8401 tgaagtacgt aaagaagaat tggtgaatac tttaccaatc ctcttgaacc aaggcgaata
     8461 ataagcgtta aaacacagaa aataaaaata gctgaatcag ctgtttttga caatctgatg
     8521 attcgctttt aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt aatgtaaaac
     8581 tcagaaaaaa cgagtatact aaaaggtact gaaaaagaag aggataagat gcgatcaatt
     8641 gcattgtttc tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat ttatttgaaa
     8701 aactgttgga acaaatccaa ctagaggaag cagaaaaaaa tcatccactg cttactgctg
     8761 gtgaaatgga tagtgtagtg gtccatcgta aatcgcgttt atgggaattt acgctgcatt
     8821 tttccaaaat tttgcctatt atgctatatc ggtcattgac tcagcatctg actatcgctt
     8881 ttaaagatat tgcacaagtc aaactgaata tcttagcaga tgaccagaca ttcgatgaac
     8941 agctcttgca agattactgg gcccaagctt tggaaaatca gcaatgcgat acaccactag
     9001 cacagcaagt attgaaaacg caggtacctg tgataaaaga tcgtaaagtc atcttgccaa
     9061 tcgacagtac aggagctatt tcttatttga agcaacaata cttgccatta gtagaagagt
     9121 tgttcgtcag ttacggattc ccaaaatttc gtatcgaacc agaagtggat gagcagcaag
     9181 cggaacgagt attgaaactt tttgaagaac gcaaacaaga acaggcagaa gcctttatga
     9241 agcaagcagc tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga aaagagcaat
     9301 cgccagctct tgaaggaccc atccacatcc agctaggacg aaatattcca gccgatgaac
     9361 caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt gaggggtacg
     9421 tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact ttgaaaatca
     9481 cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag
     9541 tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt caagaagata
     9601 cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa catacacctc
     9661 gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat agcaatatga
     9721 gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcaggg aaatggggac
     9781 accgagctat cgccattaca gaccacggtg gtgcgcaagc ctttccggaa gcacatagtg
     9841 ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc gtagatgatg
     9901 gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca tatgtcgtat
     9961 ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag ctagccgcag
    10021 tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat ccaggacatc
    10081 ctttgtcacg aacaacggta gatctgactg gtatcacgga tggaatggtt cgaggatcaa
    10141 agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca attttggtag
    10201 cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg tatggtattc
    10261 ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg tatcctcaat
    10321 tcaaacgttt cggattaggt gtgttatcta aaaaatttgg tgtcagcttg gaacagcatc
    10381 accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt gtgaaagagg
    10441 caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc ggagaaggag
    10501 attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat caggcaggac
    10561 tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac
    10621 gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg
    10681 ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca agaaaccgag
    10741 caaaattcta cgattatatt gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc
    10801 aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg gtagaaatcg
    10861 gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg aacgaagaag
    10921 atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc
    10981 atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca tttcactttt
    11041 taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc gcagatatct
    11101 gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca ggttcagaag
    11161 atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat ccgttacctg
    11221 agatcattca aaaacgatta aaaaaagagt tgaattctat taatggtaat ggcttctcgg
    11281 ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc tatctagtag
    11341 gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc actgaagtta
    11401 atccgttagc ccctcattat tattgtccag agtgccaata ttccgagttt tttgaagatg
    11461 gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct
    11521 taaacaaaga cggtcatgat attccatttg agacattttt aggattccat ggagacaaag
    11581 tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat aactatacga
    11641 aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca gtcgccgaca
    11701 aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag tttcgaagtg
    11761 cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc ggccagcatc
    11821 caggggggat catcgttatt cccgattata tggatgtcta tgattttacg ccgatccaat
    11881 atccagcaga cgatcaaaat tcggaatgga aaacgactca ttttgatttt cactcgattc
    11941 acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt attcggatgc
    12001 ttcaagattt atcagggatc gatccacaaa cgatccctac ggatgacccg gaagtgatgc
    12061 ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat tccaaaacag
    12121 ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgcta gaagaaacgc
    12181 atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt acagacgtat
    12241 ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg gaagtaatcg
    12301 gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc gggatggcat
    12361 ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat gaattgcgag
    12421 aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc gattcttgtt
    12481 caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg atggctcttc
    12541 gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac ttttctgtac
    12601 gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa
    12661 tgaaagaaat cacggacaaa ggattagatg cttccgtaaa agaaaagaat caactgacag
    12721 tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg attgatttgt
    12781 ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca ccatttagag
    12841 ctgtcccaag tttgggaacc aacgtggcaa aacaaatcgt tgaagcaaga aaagacggtc
    12901 catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat
    12961 atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta tcgttattcg
    13021 atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt atggtatagt
    13081 tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc tttttaatgg
    13141 aattacgcac ttaatttttt aaggaggcga aacttttgag tagcgtcgtt gaaacagtca
    13201 cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa gtcgaatttg
    13261 taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc ggtatcgata
    13321 tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca gcagatccag
    13381 atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag cggccattga
    13441 aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc ttataccaac
    13501 cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct gaacagctta
    13561 cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga aaaaatattg
    13621 caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa aatgagtaaa
    13681 gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg
    13741 atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg ccaagcacaa
    13801 aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa
    13861 gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc acttttaatc
    13921 aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa agattttggg
    13981 cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga agcagaaaga
    14041 acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa
    14101 cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa
    14161 caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt ttatgtttcc
    14221 cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca tccagaccta
    14281 ttgcgccgct tatttgagca agaagtacca gaagtctatg atggaatcgt tgaaattgtc
    14341 agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat
    14401 atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc aatcgtcaat
    14461 gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc tgtattcatt
    14521 gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac aaatccaaag
    14581 gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa acgcggacaa
    14641 aacgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc agaatctgac
    14701 atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa
    14761 gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt caacaatgaa
    14821 acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat gaaacaaagg
    14881 aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa agagatggtc
    14941 cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa aatgcctgga
    15001 cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga taaaaaaatt
    15061 ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact tcttgactat
    15121 gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt aatcggcaaa
    15181 aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac
    15241 tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca accgatgcaa
    15301 gcgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt ccttgctttg
    15361 agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag gtattcggaa
    15421 taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag gaaggtgatt
    15481 gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct agtaaagacg
    15541 tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt gcgattacta
    15601 gcaatgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg
    15661 cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa
    15721 caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc ggtcaaggga
    15781 gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg ggaaatacca
    15841 gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact aacaataaca
    15901 acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat cgttataata
    15961 aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca ccacgcaaat
    16021 tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca gatattgcga
    16081 aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg
    16141 tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg gactatggca
    16201 tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc ttcgaggcag
    16261 aagaagtcga tccagataaa ttagtctcac gtccgccagt cgtaacgatt atgggacacg
    16321 tagaccatgg gaaaacaact ttattggata cattacgcca ttcacgtgta acaagcggcg
    16381 aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat ggtaaaccaa
    16441 tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa
    16501 gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg ccacaaacag
    16561 ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct gtaaataaaa
    16621 ttgataaacc aggtgcaaac cctcaacatg ttatgcaaga attaagtgag tatgagttaa
    16681 ttcctgaagc atggggtgga gaaacgatct ttgttgagat ctcagcgaaa ttcggccaaa
    16741 acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat ttaaaagcag
    16801 atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc
    16861 ctgtgtcaac cttacttgtc caagaaggaa cacttcatgt tggtgaccca atcgttgtcg
    16921 gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc gataaagaag
    16981 caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc
    17041 gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa cgcgcgaaac
    17101 gtgcattatt agaaaaccgt gctgccacaa gccgtgtgac tttggataac ttatttgaaa
    17161 gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat gtacaaggtt
    17221 cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa
    17281 tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca gcagccagca
    17341 acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg caagctgata
    17401 ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa gaaatcgaaa
    17461 ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg
    17521 ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat gtaacagaag
    17581 gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta atctatgaag
    17641 gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa atgggttatg
    17701 aatgtggtgc aatgattgaa aaattcaatg acatcaaagt agatgacgta attgaaggat
    17761 ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa tcattatggc
    17821 taactaccgt gaccgccgag tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg
    17881 caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga aagttacggg
    17941 ggacttacag caagcaacga tctattacag cattttatct gacttagcat cagataagaa
    18001 aacagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt tagggcacaa
    18061 tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg
    18121 caaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa ctcccgcaaa
    18181 tcctgtgaaa tcaggggttg cgggattttt ttgttttcat taagacgtat tacctaagag
    18241 ttccatgact ttagtcttgc ggcgtgtcag ctaaccaaaa aagagaaaaa gaaaagtaaa
    18301 aaattcgact ttcttttttc tagtagtata taaatttttg catatagata aagatcaaga
    18361 taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat agccaagtaa
    18421 ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg ttgacagtta
    18481 aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga tttggtcacg
    18541 atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac caattcacat
    18601 attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag gaattgctcg
    18661 atccagcccc agttttccag caatgtgtta actggcccag cgtcattcag caaattatgc
    18721 atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag ttttttggat
    18781 caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct gttcatttgg
    18841 ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat tgggagtatc
    18901 tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt ctagtaaaga
    18961 atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg ttgggcaact
    19021 ttatcttaaa gataacccct tactgagacg tacactgaaa cccgaagatg tcaaaaaaca
    19081 tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc atttgaaccg
    19141 agtcatcaat aaatatgatt tgaacatgtt ttatattgaa gggccaggac acggcggaca
    19201 agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc cagaagtaac
    19261 ggaagatgag acaggaatgc aaaaattatt caaacgattt tcatttcctg gaggcattgc
    19321 ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc ttggatactc
    19381 tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg ctgtcgtgat
    19441 tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa atgtctttat
    19501 caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg caaaaatagc
    19561 caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact ttaacggact
    19621 tggctgggaa cctttcttta ttgaaggaaa tgattcagaa aaattaaatc ctgttatggc
    19681 tgaaaaaatg gatcaagcaa tcgagaaaat taaatccatc caaaaagaag ctcgcctgaa
    19741 gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca caccaaaagg
    19801 atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc gagcacacca
    19861 agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac tccgttggct
    19921 gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag aagaaatcag
    19981 agaaatcgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca atggtggaat
    20041 cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat ttgaaaaacc
    20101 aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg agatcattga
    20161 gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa accgattgaa
    20221 tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag aaaatgatga
    20281 atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc aagatgaagg
    20341 gttcttagaa gggtacgttc tgactggacg gcatggtttt ttgcaagtta cgaatcattc
    20401 ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatgcggaa atcacatgac
    20461 ctttcttggc gaaatgatta tccttcgcta aatctcattg caagctcaac agtcttccag
    20521 caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt ggcagaaaag
    20581 aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct cgcagtcatg
    20641 gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa acatccgcgt
    20701 gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt gaaaatcatt
    20761 gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc tgcaggaacg
    20821 gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt cccaaaacta
    20881 aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag tcaagatccg
    20941 cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc gatcttattt
    21001 gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca taaccatcat
    21061 ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga catgcgtgta
    21121 gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat caaaggaagt
    21181 caagcagaag attttgcgaa aaaaatggat cagaaattac aagaacatca aaactatatt
    21241 cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa tcttgaccaa
    21301 taaaatgata aaatcgatcc cgcaaaatag aattttttac attgattaag aaactgaaaa
    21361 aataaataaa aaaaacggaa aaaagactgg aagaatggcc agtctttttt ttcgtttttg
    21421 ggaagtgaaa accaaactat ttacctgtaa gccacccata aatccaatca aatggtgcaa
    21481 agaaaaaatc ttccattcta ttccctcctt taaaaaagca taacatgatt tctatttacg
    21541 tgagcatgaa ttttgttacg tttttattgc cgaaaaataa aaaccgttca ttatgatgtc
    21601 aaattcgtca gtaattgatg caatgtgtta taataattca gttaaatcaa aaatgaaatg
    21661 gagaaaagac atggacgggt tattaccttt atggaaagaa cgcggaatga caagtcatga
    21721 ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa atcggacatg ggggtacgct
    21781 tgacccagac gtagacggtg tgttgcccat ttgtattgga aaagccacaa aagttatcga
    21841 atatttgaca gattctggga aaatctatgt aggcgagata actctaggtt tcagtacaac
    21901 gacggaagat cgatccggag aacgggtaga aacaaaacca gtagatatgt ctttatctga
    21961 aaaaaaaata gatgaagtga tggcttcatt tattggtgag atcacccaga taccaccaat
    22021 gtactcagcg gtaaaagtca acggtaagcg gttatatgaa tatgcccgca ataatgaaac
    22081 agtggagcgt cctgttagaa aggctcagat ttaccgtttt atccggacaa gtgaattgac
    22141 aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg ggaaaggaac
    22201 ctatgtccgc acattagctg tagatacagg caagaaactt ggttaccctg cgcacatgtc
    22261 tgatctgacc cgaacagcaa gtgccggtat gtcccagaag gatgctctga cattagaaga
    22321 agtagctcaa gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa tagaaaaagg
    22381 agtggaacgg ttcaaacgag tagatattac tgatgaagta tggcagaaag taaaaaacgg
    22441 tatgagactt gattatactg tatttgcgct cccagaaatg ccgtcagaag aaattgcttt
    22501 gttttataaa ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa aaaacaaatt
    22561 aaaacctagt aaagtattga gaaatgagga ataacgaatg aatatcatca agatcagaca
    22621 tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg gatttttcga
    22681 cggcgtgcat atcggacacc agaaagtcat aaagacaggt atggaaattg cacaaaaaga
    22741 aggattgaaa ttagcattga tgactttcaa tcagcatcca tctattgtct ttaaaaagat
    22801 cgatcctagc agcgtgaaat atttgactag ccttaagcaa aaagaagaaa aaatggccgc
    22861 tttgggtata gattatttat atgaagttga ttttacttct tcttttgctc atttagcacc
    22921 gcaggaattt gttgatcaat acatcgtagg gttacatgca aaatatgctg tttccggttt
    22981 tgattatacg tatggaccaa aagacattgc agatgtcgct cattttcctg aatatgccaa
    23041 aggaagattc aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa aaatcagctc
    23101 tacccgtatt cgtggattac tagacagcgg acaagtaaaa gaagcagcaa aactattagg
    23161 ttgcacatat gaggtagaag gggtagttgt ccatggcgat gcacgaggca gactactagg
    23221 attccctaca gcaaatgtca aagtaaagag cacggttcat ttgcctaaag aaggtgttta
    23281 tgtgtcggaa ataaaaattg gggaaacatg gtatccagcc atgggttcaa ttggacataa
    23341 tgatacattc ggtgaaggac gggagctgac tgtagagttg tatattttgg actttaatca
    23401 agatatctat ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag atcaagtgaa
    23461 attttctagt gtagaggaat tgattgaaca actcaaggag gatgaacaag caacagctga
    23521 atatttttct cagcaataaa acattatgca taaaatctct tgtttatgta taactagcaa
    23581 ttacaataaa atgggtgata aataagccca ggaaaggggc tgtgacaaaa gtcttgtcac
    23641 agcctttttt tccaaataaa tggtttttca gaagtagctt cttcggaaat aagccgaaat
    23701 ttcttgaagc tgaccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
    23761 caattcatga aaacgattgc tagagcgtat catttgcttt agactatata tcttggtgta
    23821 cactcacttt aaggcggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
    23881 agtgggtact atgtacaagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
    23941 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
    24001 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
    24061 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
    24121 tacggcttta gaaaattttg ggacactgga cgtttgggtt aataatgctg ggatggaaaa
    24181 tcagcatcca acccatgaac ttagccttga agattgggaa agagtgttaa aagtcaattt
    24241 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga ataataaaaa
    24301 aggaaatatc atcaatatgt cttctgttca tgaacagatc ccatggccaa cgtttgctca
    24361 ttacgctgct tcaaaaggcg gggtaaaatt atttaccgaa acggtagcaa tggagtatgc
    24421 accgaaggga atcagaatca atagtatagg ccctggtgct atccgtacac ctattaatgc
    24481 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
    24541 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
    24601 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
    24661 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
    24721 tggctggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
    24781 attaggaact ggtattggag cggtgcttgt cggcttactc gttcaattat tcgcttctcc
    24841 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tttgggttat
    24901 tggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
    24961 ttcgactgca ttacagttag tcggaacctc gttgattggt gtcttcgctt ttggagaatg
    25021 gtctggaagt atggggaaaa tcattggagc agcagcaatc attcttcttg tatttggttc
    25081 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaattag
    25141 tctattagca tcaacaagta ttggttattg ggtatacagt gcattgccta aactagttga
    25201 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
    25261 ttatgtgatt ttccgtaaac caaccgcatt ttctgaagac aagagttgga aagcaacgat
    25321 cgttggagcc atctttagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
    25381 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
    25441 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
    25501 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaattt
    25561 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
    25621 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagggaaa
    25681 tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg acataattga
    25741 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
    25801 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggaattgat
    25861 tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt gcatttagca
    25921 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
    25981 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
    26041 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
    26101 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
    26161 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
    26221 cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag tgatattata
    26281 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
    26341 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtaga
    26401 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
    26461 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
    26521 aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
    26581 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
    26641 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
    26701 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
    26761 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
    26821 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
    26881 aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga tcggctgaaa
    26941 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
    27001 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
    27061 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
    27121 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
    27181 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
    27241 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
    27301 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
    27361 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
    27421 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
    27481 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
    27541 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
    27601 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
    27661 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
    27721 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
    27781 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
    27841 aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag attctcgatt
    27901 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
    27961 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact aaccttattg
    28021 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
    28081 gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg tggccccatg
    28141 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
    28201 gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg atcaggcatt
    28261 taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
    28321 cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga agatcacttg
    28381 ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
    28441 cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg tcaaaggaat
    28501 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
    28561 ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
    28621 ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat ctaaatagaa
    28681 aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac atattagaaa
    28741 gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg atttcctgta
    28801 ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct aatgaacgct
    28861 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
    28921 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
    28981 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
    29041 gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa agtattacct
    29101 ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
    29161 tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga aatatttgtc
    29221 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
    29281 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
    29341 tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
    29401 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
    29461 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
    29521 accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga aaaagtgtga
    29581 caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt aattgaagag
    29641 aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc aagtttgtat
    29701 agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg aagaatcaag
    29761 tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc tttcacagct
    29821 tgttgttcta taaaatcttc tttattctga taattttccg attctaacca aggaaattgc
    29881 gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata atcaactgcc
    29941 ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat ttgatataaa
    30001 tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc aactttttca
    30061 ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga agtgacctct
    30121 gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt gctagtaata
    30181 taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt tttcattttc
    30241 aagctctcct ttactatcta taacaaggac aacagtttta acctacagac attcattaaa
    30301 attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat acagatagtc
    30361 aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt taccagcatt
    30421 ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg agtacaatat
    30481 taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca tatcgggcac
    30541 tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat gaaagaaaaa
    30601 atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa tacgtttagt
    30661 atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag gagtaatctg
    30721 tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc agctttgcga
    30781 ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat aagcaagcat
    30841 ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag ttggtgcttg
    30901 aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga gaaagaattt
    30961 caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt catatcccat
    31021 tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa gggcagccag
    31081 tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa agtcatccga
    31141 cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta tctgctaaac
    31201 aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat ggtaatgagt
    31261 ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta atgaaaaact
    31321 atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta ttgaaaaaaa
    31381 ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta gaagcagaaa
    31441 attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca ctagaaggat
    31501 atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct ttgtactcat
    31561 tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga ctcgaactgc
    31621 ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg gaaaagcatg
    31681 gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct caacataacc
    31741 tcatgtactg gaacaatgcc cactattttg ggtttggagc tggtgctagc ggttatcttg
    31801 gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca ttacgagcaa
    31861 atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata gaagaagaaa
    31921 tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat gatcgctatc
    31981 aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa gaagggttac
    32041 ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg gggaacaatg
    32101 tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt atctgaattt
    32161 aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat aactggattt
    32221 acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg gagtctttct
    32281 cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta gaatatatga
    32341 gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt tacgtctgat
    32401 catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga tgtctgaggg
    32461 agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag atgaaggatt
    32521 gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct atcgttatta
    32581 cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc tgattcgtca
    32641 gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg ctgagatatt
    32701 atctgagctg acaagctata cgactttttc attgggacct gaaataaaag accgccgact
    32761 gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg tcgtaacaga
    32821 tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa gtcaggaact
    32881 agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga tgacggtgta
    32941 ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa cgacagaagg
    33001 gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg tttatgtcag
    33061 tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt tcaaatcgat
    33121 gttttctttt atgaaagact cagacggact tacacaaatg atcactccga tggatagcaa
    33181 tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata tgagtatgat
    33241 tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt tagggcctac
    33301 tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag aactagcagc
    33361 taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg aataacgaag
    33421 ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc tgactgaata
    33481 atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga
    33541 tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta tttcagaagc
    33601 tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa tggaagataa
    33661 atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga
    33721 gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat caattgacaa
    33781 cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg
    33841 cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat
    33901 ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc
    33961 gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct acaaattaca
    34021 tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg gatataaaac
    34081 ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg tattgactta
    34141 ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat catcgctaac
    34201 ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga aatccaagta
    34261 ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc aatcaaacgc
    34321 catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac accacaagaa
    34381 atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt aggtgaaaaa
    34441 gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg tcaagcaaca
    34501 aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga accaactgca
    34561 gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt agtattcgac
    34621 cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt ctttgatgta
    34681 ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa aatcatcgac
    34741 tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga caaaatggct
    34801 ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc
    34861 acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca tttggaaatg
    34921 acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg tacaaaagta
    34981 ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga cgaagttatt
    35041 ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa agaaacagga
    35101 aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc tgctatccaa
    35161 ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac accattgtca
    35221 ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa tacaacgatt
    35281 ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc agtagacatc
    35341 catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg aagattccaa
    35401 ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac attcgatatt
    35461 gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa
    35521 atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat ggtaaaagat
    35581 gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct acgtaatgat
    35641 gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg caaagtggac
    35701 gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc tgttgaagca
    35761 aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt tcaaaacttg
    35821 actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc agaagcagct
    35881 caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg cgacgataaa
    35941 taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt tttcttttga
    36001 aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata acaagcgacg
    36061 tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga ttattatgaa
    36121 gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta tcgtaagctt
    36181 tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa attcaaagaa
    36241 gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata tgaccaatac
    36301 gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg atttggtgga
    36361 ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga ctctttcttt
    36421 ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat
    36481 acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat caagtataat
    36541 cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac acaaccaact
    36601 acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac accacttgga
    36661 cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga aatcaaagaa
    36721 ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt taaagtgaat
    36781 gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg agaagctgga
    36841 gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga aagtgatatc
    36901 tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt acaagcagct
    36961 ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa gatccctgct
    37021 ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa gttacgtggt
    37081 ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa
    37141 caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt tgaagaacag
    37201 caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag
    37261 tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca atcatatgtt
    37321 cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aaagccagct ttagaatatg
    37381 ataatcgtca tagtttctag gggtataacc agtgagagaa tggaacttgt tcaatcgata
    37441 ttgcaaggtg tttttatgaa taaataattc ttctgagcaa tttttgatgc ttccgttatt
    37501 tttactgaag catgccaaag tcttcttgaa caaatgtact tcttcttctg tcaggctttt
    37561 caaaatacgt tttacatata atgttcgttg atctgacggg attgctggta aaacgatccc
    37621 gaggtctaaa tcttcaaaaa agcggatctc gttcacagat tgatggaaag ctatccattc
    37681 gcaaacttgt ttggcatgaa ggaaagattg cggtgtctct tgctctgtat aacaaagcga
    37741 cccaattcca aagtttagct gtaactgata cattttatta aactttgtct ggattttatc
    37801 aagcaatgat agcaatgagg ctctggaaat ccgattgact aggataatga tttgttgtct
    37861 atgtacagtg agcaaagcct gtccgtaata agagaagacc ctttcgagca actccaataa
    37921 aggattgatc cgttcttgac acaattgctg attgtcagaa agttgtccca caactacata
    37981 atatggagaa gaaaaatcaa gctcaattaa gtgagggtgg tatcgactat gtacttgata
    38041 tcatcaatga accgatggta ctttcttatg cgatggaaca ggtagagcga ttgcttgttt
    38101 gtgcaggaaa acaagcgatg cggctgatac gattaggtga gaaaataaga agataaaaat
    38161 acacggtccc ttcatggcga ccgtgcattt ttggttttta ttaaatattt aatactttat
    38221 tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa gatttgttga ggcgttccat
    38281 cttctaaaaa gtttccgtcg tcaatgaaca tgacgcgatt ggccacttct ttagcgaacc
    38341 ccatttcgtg cgtaacgata accatcgtca taccttgttc tgctaacttt ttcatgacgt
    38401 tcaagacatc accgaccatt tcaggatcga gtgcagaagt aggctcatca aaaagcatga
    38461 tgtcagggtt catcgctaga gcacgagcaa tagccacacg ctgtttttgt ccaccagaaa
    38521 gcatttcagg atacatatct tttttatctt ctagaccaac tgtctgcagt aatttgattg
    38581 cttttttctc tgcttcttct ctagattctt tcttcaggtc catcggtgct aaaatgatgt
    38641 tttccaaaat agaaagatga gggaataggt taaagtgttg gaataccatt ccgatatgtt
    38701 gtctcaccaa gttgatatct gtgcttttat ccatcaagtg ggcgccgtca ataataattt
    38761 caccgtcaga aggttcttct aaacgattca agcaacgcaa gaatgtactt tttcctgaac
    38821 ctgaagggcc aatgatacag acgacatctc cttcgttgat ggatacattg atgtcgttta
    38881 atactgtgtt atcgccgtat tttttgacta aatgctcaac taagattttt tctgccatct
    38941 tacttcacct tcttttctaa aacttttgct aattttgtca atgctgtgat caaaatcaag
    39001 tacatgatcg caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc aacaatgatc
    39061 ttaccagttt gcagcaattc gactacaccg attgcggaaa tgatcgttgt gtcttttaat
    39121 gagataacga actgattgac gaatgatggg atcatgatgc gtatcgcttg cggcaaaatg
    39181 attttttgca ttgtccgatt ataagaaaga cctaaacttc tggaagcttc catttgtccg
    39241 acaggaacgg cgttgatccc gcctcgaaca atctctgcga tataagcact tgcgttaaga
    39301 gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa atccaacgat accaggcaac
    39361 ccaaagaaaa taaagaacgc aagaaccatc attggaattc ctcggatgat atcgacataa
    39421 atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc aaaaatcaca
    39481 ccgattacta tagccaaagc aaaggagatc aaagttagga gaattgttct ccaaagtcct
    39541 ttcaacaatg cttgatagtt gttttggata agtccaagaa atgttgattc atcagcagtg
    39601 gaagactcac tggaagtttc tgaaccatcc gctacatatt ctttaataat cttatcgtat
    39661 tcgcctgttc tttttaattc tttcaatcct tcattgaaca tattaagaag ctcaggattt
    39721 tgtccttttt taacggcaaa tgcatatttt ccgccagatt cttttttgat cggtgtttcc
    39781 aattcttgtc cttgtttgat cgcataaccg attaccggat aatcatccat cattgcatcg
    39841 attgcgccaa cttttaaccc gtcgtacaat tgatcagcag catcaaaata tttgatagaa
    39901 tagccatatt cagattcatg gtcgcttaaa aattcagcac tttcggtacc aacttttgct
    39961 ccgactgttt ttcctttcaa atcttcataa gacttgatcg aagtatctcc ttttttgaca
    40021 gctaattgga tcccgctatc aaagtaagaa tcagaaaaat catagctttt ctttcgttca
    40081 tctgttacag tcattccggc aatcattccg tctgcttgtc ctgattccaa agcttgcaaa
    40141 gcagcagaga aaccacggta gtccattcgg atgttaaatc cttgcagttt ggcgatgttt
    40201 tccatcaatt ttacatcgat gccttcgtac tcgccctttg cgttttgaaa ttcaaatgga
    40261 gcaaaagcag agtcactggc aattacgtat acgtcttttt tgggctctat tttttgcatt
    40321 gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac atgaccaaac
    40381 gcaagtgtca caagtcctgt cattaagaaa acaaatgagt gaaataaatg ctttcttttc
    40441 ataatcaacc tcctaaattt ccaatatttt attattatag gcgaaaagtg ctgaatcaga
    40501 caagactgta agctcgaaaa aatgaaaaaa atgcgagttt ctaacatttt atgttataaa
    40561 acataacatg tataataaga taaaaaagtg gcgaaagtat gttcggtgtg gtataatttt
    40621 ttagtaagga atgcctttgc tttccttttc tttttattgg tcgtaaaatg aaaaggaatg
    40681 aatgaaaagg aggacgaaga atgattgaga gagatacctc ccgacacttt gagcttgtct
    40741 cgaaatacca accggctggt gatcagcctg aagcgattaa ccaattagtg gatggcgtag
    40801 tcggcgggaa aaaagcacaa atcttattag gtgcgactgg aacagggaaa acatatacga
    40861 tttccaatct tattgagaaa gtgaataaac caacactgat tattgcccat aacaaaacat
    40921 tggctggtca gctgtacggg gaattcaagg aatttttccc taacaatgct gttgaatatt
    40981 ttgtttctta ttatgattat tatcagcctg aagcttatgt gccttcaagc gatacgtata
    41041 tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt acgacattcg gctactagtt
    41101 cattattaga aagaaatgat gtgatcgtca ttgcatccgt ttcatgtatt tttggattag
    41161 gctcgccttt tgagtaccaa aaacaagtag tttcgattcg acagggagca gaactagatc
    41221 gcaatcagtt gattcgtgac ttagtcagta tccaattcga aagaaatgat atagattttc
    41281 agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat ttttccggct tcaagagatg
    41341 aacgtgcctt acgtgtggag ttttttggag atgaggtcga acgtatccga gaagtcaatg
    41401 cactgacagg tgaagtttta ggagaaacgg aacacgtagc aatcttcccg gcgactcact
    41461 ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa tatcaaagcg gaattagaac
    41521 agcgtttaac tgtcttgcga aatgaaaaca aacttctgga agctcaacgt ttagaacagc
    41581 gaaccaatta cgatattgaa atgatgttag aaatgggcta cacgtcggga attgaaaatt
    41641 attctcggca tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta ttggatttct
    41701 ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt aaccatgcct caaatcagag
    41761 gaatgtataa tggtgaccga gcgagaaaac agatgctagt agattacggt ttccgtttgc
    41821 catctgcttt agacaatcgg ccacttcggt tagaagagtt tgaaaaacat gtcaatcaga
    41881 tcatttatgt ctctgcgacg ccaggaccat atgaacatga acaaacagat accgttatcc
    41941 aacaaatcat ccgtccgaca gggctgcttg acccagtgat cgaagttcgt ccaatcatgg
    42001 gacaaatcga tgatctagta ggcgaaataa atgaacgagt cgaaaaagac cagcgggtgt
    42061 ttgtaacgac attgacgaaa aaaatggcag aagacctcac agattatttc aaagaattag
    42121 ggatcaaagt caaatatttg catagtgata tcaaaacgct ggaacgaaca gaaatcattc
    42181 gagatcttcg attaggtgaa tttgatgtgt tagttggaat caatttgctt cgtgaaggat
    42241 tagacgtacc tgaagtttcc cttgttgcta tcttggatgc ggataaagaa ggctttttac
    42301 gtagtgaacg ttccttagtc caaacgattg gacgagctgc tcggaatgaa gaaggtaaag
    42361 taatcatgta cgcagacaaa gtaacagatt ctatgcgtct agcgatggat gaaacgtcaa
    42421 gacgtcgtac gattcagcag aagtacaatg aagaacatgg gatcgttcca aaaacgatca
    42481 ttaaagaaat ccgagatttg atttctatta cgaaagaatc ggaagatgat acaaaagaag
    42541 cggtacaagt ctcgtacgaa gaaatgacga aggaagaaaa agacacgtta ttgatgaaat
    42601 tagaaaaaga aatgaaagat gcggcaaaag ctttggattt tgaaacagcc gcgaatgtgc
    42661 gagatatgat tttagaatta aaagcttcaa agtaggagga caacatggct aacgataaaa
    42721 ttacgattca tggtgcacgt gcccataact tgaaaaatat agatgtaacg attccacgag
    42781 ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa aagttcccta gcatttgata
    42841 cgctgtacgc tgaagggcag cggagatacg tagagagcct ttctgcttat gctcgtcagt
    42901 ttttaggaca aatggataag ccggacgtag atagcatcga cggattgagt ccggcgattt
    42961 ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact gtgacggaaa
    43021 tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt cccaacgatc
    43081 atatcgagat taccagtcaa tcagtagatc aaatggcaga tcaagtaatg gctttgcctg
    43141 agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga caacataaaa
    43201 aaatattcga acgaatccaa aaagagggat acgtccgcgt tcgagtcgat ggtgagatct
    43261 atgatttaag tgaagtgcct gagctggaga aaaacaaaaa acatgatatc gcgatcgtca
    43321 tcgaccggat cattgtgaaa gaagggattc gttctcgcct atttgattct ttagaggcgg
    43381 ccctgcgatt agcagacggc tatgctctgg ttgacgtgat cggtgaagaa gaaatgttgt
    43441 ttagcgaaca ctatgcctgt ccttattgcg gatttactgt tggcgaacta gaaccaagac
    43501 tgttctcatt caatgcgcct tttggagctt gtccagactg tgatggttta ggtgtcaaat
    43561 tagaagtcga tcaagattta gtgattccag atagaacgaa gacacttagt caaggtgcta
    43621 tcgttccatg gaatccaatc agctctcagt attatcctca aatgttagcg caggcttgtg
    43681 aaagtttcgg tatcgattta gatacaccat tcgaagaatt accggaagag catcaggaga
    43741 tcatcttgaa tgggacagat gatcttttcc atttccatta tgaaaatgat tttggtggcg
    43801 ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa tatcgagcgt cgttatcatg
    43861 atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa ttaacttgtc
    43921 agacatgtaa aggttatcga ttgaatcccc aagctctatc tgttcaaatc aatggaacga
    43981 atatcgggca aacaaatgag ttgtctattc aaaaagcagt agatttcttc tcaaacttaa
    44041 cgttatctga acaagagaaa gtaattgcca aaccgatttt aaaagaagta aatgacagac
    44101 tttcattctt ggaaaatgtg ggactagact atctaacttt gagtcgcgct tccggaacat
    44161 tatctggggg agaagcgcag cgaatcagat tggctacaca aattggatcg aatctttctg
    44221 gagttttgta tattttagat gaaccatcga tcggattgca tcaacgcgac aacgatcgac
    44281 tgattggctc attaaagaaa atgcgtgatc ttggcaacac cctcgttgta gtcgaacatg
    44341 atgaagatac gatgtgggca gcagattatt tgattgacgt cggacctggg gccggagaac
    44401 aaggcggaca aatcgtggct gcaggcactc ctgaagaagt agctaatgat gaaaattctt
    44461 tgacagggaa atatctttca gggaaaaaat caatccctgt accaaaagaa cgccgtaaag
    44521 gtaatgggaa agcaatcaag atcactggtg cttcagaaaa caatctgaaa aatattagtg
    44581 ttgaatttcc attaggagaa tttgtagcag tcacaggtgt gtctggttct ggaaagagta
    44641 cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa attaaataag aattctgcta
    44701 agccaggtaa attcaagaca atttccggct acgaaagtat cgaaaagatc atcgatatcg
    44761 atcaaagccc aatcggccgg acgccgagaa gtaatccagc gacttataca agtgtatttg
    44821 atgatatccg tgggttattt gctcaaacga acgaggcaaa aatgcggggt tataagaaag
    44881 ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg gatggaatta
    44941 ttaagatcga aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta tgtcatggta
    45001 aacgatataa ttctgaaaca ttagaagtgc attacaaagg aaaaagcatt gctgatattt
    45061 tggaaatgac agtagaggat gctgtagaat tcttcaagca cattccaaag attcatcgca
    45121 aactgcaaac gattgttgat gttggcttag gttatgtgac gatggggcaa ccagcaacga
    45181 cattgtccgg tggtgaggca caacggatga aacttgccag tgaattgcac aaaatctcta
    45241 atggaaagaa tttctatata ctagatgaac caacgacagg acttcatagc gatgacatcg
    45301 cccgcttgtt gcatgtatta caaagattag tagatgctgg taacacagtt ttagtgattg
    45361 aacacaatct agatgtaatc aaaacagcag attatatcat tgatttagga ccagaaggtg
    45421 gagaaggtgg aggaacgatc cttgcgactg gaacaccaga agaaatcatt aacgtaaaag
    45481 aaagttatac aggtcactat ttgaaaaaaa taatggtata aagaggatag atagctgaga
    45541 tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt attaatatca gtgttctaga
    45601 atcattacat ggtagttctt tctacactga ttggtataga taaaaacttc ctataagttc
    45661 aatccgttca aaaaagaatt gagcaatcgg aggactttcc tttgattgct tttttcttta
    45721 tggtatgata ttccttgtgt agttggaaaa gaaaaacaag agattatgag taagtgagag
    45781 ataatgatga acagtattga aaatgaagaa tttatcccta tattattagg aagcgacatg
    45841 aacgtttatg gtatggcacg ttcttttaat gaagcatacg gcaaaatctg ccaagcatat
    45901 gcttcggatc agttagcccc tacccgctac agtaaaattg tcaatgtaga agttattcca
    45961 ggatttgata aagaccctgt atttatcgag acgatgcttc gtttggcaaa agaaagatat
    46021 tctgataaga gtaaaaaata tcttttgatt gcttgtggtg atggatatgc agaattgatc
    46081 tcccaacata aacaagagct gtcagaatat tttatctgtc catatattga ttattcttta
    46141 tttgaaaggc tgatcaataa agtaagcttt tatgaagttt gtgaagaata tgatcttccg
    46201 tatcctaaaa cattgatcgt cagagaagaa atgttagtaa atggacattt ggaacaggaa
    46261 ttgccttttg aattcccagt tgctttgaaa cctgcaaaca gtgtggagta tctttctgtc
    46321 caatttgaag gaagaaaaaa agcttttatt ttagagacta gagaagagtt cgatttaatt
    46381 ttaggccgga tatacgaagc cggttataaa agtgaaatga ttgtacaaga ttttatcccg
    46441 ggtgatgata gtaatatgcg tgtgctaaat gcgtatgtag atgaagatca tcaagttcgg
    46501 atgatgtgcc ttggtcatcc actattagaa gatcctacac cagcatcgat tggtaattac
    46561 gttgtgatca tgccagatta taacgaaaaa atctatcaaa ccattaaggc atttttggaa
    46621 aaaatcgaat atacaggctt tgccaatttc gatatgaagt atgatcctag agatggagaa
    46681 tataaattat ttgaaatcaa tttgagacaa ggtcgtagca gtttctttgt gacattgaac
    46741 ggcttgaatc tggctcgttt tgtcacagaa gatcgagtgt tcaacaaacc ctttgttgaa
    46801 acgacctatg gaacgaatca atcagataaa gcaagattat ggatgggtgt gcccaaaaaa
    46861 atctttttag aatatgctcg ggaaaatgaa gacaaaaaac tagctgagca aatgatcaaa
    46921 gaaaatcgtt acggtactac tgtattttat gaaaaagacc gatcaatcaa gcgctggttg
    46981 ttgatgaaat atatgttcca taactatatt ccaagattca aaaagtattt tcatgtgaaa
    47041 gaaggctagt tcatggagaa ttttttcagt attttaggcg gaatgggcac gatggcgaca
    47101 gaaagttttg ttcgcttgat caaccaccga acgaaagcta caaaagatca agaatatttg
    47161 aattatgtct tattcaatca tgcaacagtt cctgatcgta cagcttatat tttagatcga
    47221 tcagaagaaa atccaatgcc gtttctgctg gatgatattg agaaacaaaa tttattgcga
    47281 ccaaacttca ttgtgttgac ttgtaacacc gcacactact tttttgaaga gttacaagcg
    47341 gcaacggata ttcctatctt gcatatgcca agagaagcag caaatgaact tgtccgtcag
    47401 catacgacag ggagagtagc gattctagga acagaaggaa gtatgaaagc tggaatctat
    47461 gaacgcgaag tcaaaaatct tggtttcgaa acagtgattc ctgatactgc gcttcaagaa
    47521 aaaatcaatt acctgatcta tcatgagatc aaggaatcgg attatttgaa tcaagagctc
    47581 tattacgaaa tcttagagga agcggtagag cgcctaaact gtgaaaaagt catcctaggt
    47641 tgcacagaat tatctttgat gcatgaattt gctgaagata atcattaccc agtgatcgat
    47701 gctcaatcta ttttagcgga tcgaacgatc gaacgtgcat tagctgaaag aagcgaggca
    47761 ttggatacag catcggaaaa gtaaaaaaat tggatttggg gcaaatatcc taaaagatat
    47821 ttggtccaaa tctttttgta aaagaaaaag aaaactggta atgatacgcc cgggatgata
    47881 taattttatt atgcacaatg tgagaggggt tcgtaaatat gacagataat ttgcaattag
    47941 tcatcataac aggtatgagt ggtgcaggga aaacagtagc tatccaaagt tttgaagata
    48001 tgggctattt ttgtatcgat aatatgccgc ctcgattgat tccaaaattc tgggaattga
    48061 tcaaagaatc aggtaaagtc acaaaaatgg ctttaggagt ggatttgcgt tctcgttcct
    48121 tctttgaaga aatccaaaac atgctgatcg aaatcgaaaa tacgaacttc atcgacactt
    48181 ctatcttatt tttagatgct tcagacgaag aactagtggc tcgctacaaa gaaacaagac
    48241 gagcacatcc aatggcgatg gacggtttga tcactgaagg gatacgtaaa gaacgggcga
    48301 tactagacga cttgaaagcc caagcttccg tgatcattga tacgacaaca ttgacaccac
    48361 gacaattacg tgaaaaaatc aatgagtcat tcaaatcaaa gggagaccaa ggatttcgga
    48421 tcgaatttgt atcatttggt tttaaatacg ggttgccgat cgattcggat attgtgatgg
    48481 atgtgcgttt tttgcccaac cctcattata tcccggaact aagaccattg accggaaaag
    48541 atcagccagt ctatgattat gtgatgtcct ttgatgaaac gcaatcattc taccataaat
    48601 ttttggatct tctggaaact gtattgcctg gatacattaa agaaggaaaa agcagcttga
    48661 cagttgcaat cggctgtaca ggcggtcaac accgttctgt ggcattgact gaacgtttgg
    48721 caaatgcttt agggaaaaac tataaagtca atgtgacaca tcgggacaag gataaaagga
    48781 aagagacggt gaaccggtcg tgaaaatgaa aacctaccgg ataagaaaac caaagatcgt
    48841 agtggtcggc ggtggcactg gtctgccagt tatcctgaaa agtttacgaa atcaaggagc
    48901 agatatcaca gcagtagtga ctgttgcaga tgatggtgga agcagcggtg caatcagaga
    48961 atcgatcgct atggctcctc ctggtgactt gagaaatgtc ttagtggcgt tatcggatat
    49021 gccacaattc tatgaagata ttttccaata ccgtttcaaa aaggaagatc agtttttagc
    49081 caatcatacg atcggtaatc tgatcattgc cgcagtttcc gagatgcggg gaagcactta
    49141 tgaggcgatt caattattag caaaaatgat gcatgtagat ggacatgttt atccttcttc
    49201 agaaactcct ttgaccttgc atgctgtttt taaagacgga acgactgcag taggagaatc
    49261 taaaatcgcg atcaaccgta aaaccattga ccgtgtgtat gtctgcaaca caaatgataa
    49321 aagtgaagcc aaagcggctc gaaaagtcgt ttcagcaatt atggacgccg acatggtcgt
    49381 tttgggaccc ggaagtttat tcaccagtat ccttccaaat ttagtgatac cagaaatcgg
    49441 cgaagcaatg ctcaagacaa ctgctgaaac cgtctatatc tgtaatatca tgacccaaaa
    49501 aggggaaacg gaacatttca cagatgccga tcatatacgt gttttacaca agcatttgaa
    49561 tcagcctttt atcgatacag tattggtcaa tacggaaaaa gtgcctgaag attatatgga
    49621 cccagagatc tatgatgagt atctagtaca agtcgcacac gacttcaacg gattgagaaa
    49681 tgaaggatgt cgagtgattt ccaccgactt tttagaattg aaagatggtg gtgtcttcca
    49741 cgatggagac aaagtagtag aagaattgtt ccgtttagtt ttcggttcaa aatattaagc
    49801 aatttattgt tggagaaagg aggagaatag gatgtcattt gctgcagatg tgaagaaaga
    49861 actaactggt ttggaagttc atcgagaaca tgcgaaagcg gaattagctg ctttgctgcg
    49921 aatgaacggc tctttaggta ttgtgaacca gcagtttatt ttgaacgtcc aaacagaaaa
    49981 tgctgcaatt gccagacgta tctattctct tttgaaagat cattatcagg tacgaagtga
    50041 gctactcgtt cgtaaaaaaa tgaaattaaa aaagaacaat gtctatatcg tccggttgaa
    50101 gcaaggcaca aaagatgtgt tgatggatct tgatatcatg gatggtatga tgttcaatgg
    50161 acacgtttcg gacgagatta tgggaaattc gcaaaaaatg cgttcttact tacgtggtgc
    50221 ttttatggcc tcaggttcag tcaataatcc ggaaacgagt cgttatcatt tggaaatatt
    50281 ttctatttat gaacagcata accaagacat ttgcgatatg ttaaattatt acggtctaca
    50341 tgctcgaacg cttgaacggc gcaatggcta tatttcgtat ttaaaaggag cagaaaaaat
    50401 tgctgatttc ttaacattga ttggtgcaac caactcaatg ctccgatttg aagatgttcg
    50461 aatcgtacgg gatatgagaa attcggtcaa tcgtctagtc aattgtgaga cagcaaatct
    50521 gaacaagacg attgatgcgg cttctaaaca aatcgaaaac atcgaattga tcgaagcaaa
    50581 ggtcggtcta catgcccttc ctgaaaaact tcaggaaatc gcagaacttc ggcttcaaca
    50641 tccagaggtc agcttgaaag aattagggga aatgattccc tcaggtgcaa tatcaaaatc
    50701 gggaattaat caccgaattc gcaaaataaa tgaatttgcg gaaaaactgc gtgaagaagc
    50761 agtttaatcc aaagaaaaaa ctgatacatg aagcaggaga tgcgaatgta tcagtttttt
    50821 ttaaaagcta tacttgcttt tcttccgtca aactataatt ccaactttac tggagtaaca
    50881 taatgttgaa aggcttctat tgttttttaa tacaatcgta taaaaagcgg ggagacaact
    50941 gaatgaagat tttagtttta tacataaact tttgcttaat atagaagtcc tgatatagat
    51001 aggatcaagt atttatctat aactggagat taattaaaaa ctggaaggaa ctgctcgatc
    51061 aaccttgaga actgtttttc ttacttctgg tcaagattct cgtggttggt ggataaggat
    51121 ggttgataag ttcattggat tagctagtcg aaaacaacca ggtgtaatta atgtagaagt
    51181 tttaacaagg ggatggagtg atttattcag tgataatcat aagttcataa atagtaattg
    51241 ggatgtatgg ttggtatacg ttatacctga acttgaacta tcaagatttg gtttagaatt
    51301 tgagaatcga tgtttataaa gggagggcaa aaaatgtgct tttttacaac ttttactatc
    51361 attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac agcattttta
    51421 ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt tggatttttt
    51481 ctattcacag ttattgtatt tatttggaga actacagcac caccattgcc agagattaca
    51541 tatggagaat ttccgtttag tcttgaatat gaaatagatg aggaaagaat tttgattgaa
    51601 gatacggtga ttgcagaatt tataggaagt tttagaggga atgtgacaac tcaacctaga
    51661 cgaagttgga gtataaaact tttaaataat agtggagagt tgaaagcatt tcattatgaa
    51721 ttcttaataa tagaaagtga agatgtaaca atcactttaa gtagtggctc tgctggatat
    51781 tttatgaatg atggtggaag caagccacag ctcacagtcc gtgatcgtat tgcaggtttt
    51841 atgcattttt ctcactatga cttagaagaa gctcgtaaaa tacttgcaga atatgggatc
    51901 acattaatca attgggaata tgcgccaccg ataagaaatt ttttcaatga ttattattag
    51961 tcttataaaa aacagaatgt aaaatttatg tgcgcgagta tttagttttc cattttttgt
    52021 ttgatagata tatagcattg ttgaagcatg ggatagtggt gccaatttta tcaatgaggt
    52081 tggtcaggca gttaacataa ttggtgatgc tataaatcca tttaattgga attggaggaa
    52141 ttaggtgtgg aaacagtgag attatggata ttcatgaaaa attttcgttc ggctttgaga
    52201 tatgttattc tggaatcaaa atcaggttat tatctgattg acacagatca ctatactttt
    52261 tggggaggct acctttttcc gatttttaat tggataatcc cccataaggt tatgaaaatt
    52321 aacttgcctg cagaagaaat aaatagatta atactcaaca aagatttaca aaggaaaaga
    52381 gagaaagaac attctaatgt tgcttggata ggtccagcat ttgcagtagt gacttcttca
    52441 attgtcggtc ctatgctaga gagtatttat agtgattttt ctattggaat acgttattta
    52501 tttgttactt taagtatagc tgttcttttg tgcataaagg gttatgttag caagaagaat
    52561 aaagatattt taacactaat cggtgtaaac aatttatcta gtactaaaat tatactgttt
    52621 cctgaatcta taaaagaagg atcaaggttg ttgataattt ttataatgct tatactgttt
    52681 tctgttttat ttggggtgat gtttataga
//
LOCUS       DAZUKT010000010        51519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000010 DAZUKT010000000
VERSION     DAZUKT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.010"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1208
                     /locus_tag="ACG816_000583"
     CDS             207..1208
                     /locus_tag="ACG816_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4919496.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1227..2246
                     /locus_tag="ACG816_000584"
     CDS             1227..2246
                     /locus_tag="ACG816_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4919497.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2348..3064)
                     /locus_tag="ACG816_000585"
     CDS             complement(2348..3064)
                     /locus_tag="ACG816_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4919498.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3033..3818)
                     /locus_tag="ACG816_000586"
     CDS             complement(3033..3818)
                     /locus_tag="ACG816_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4919499.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3809..4558)
                     /locus_tag="ACG816_000587"
     CDS             complement(3809..4558)
                     /locus_tag="ACG816_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4919500.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4856..5380
                     /locus_tag="ACG816_000588"
     CDS             4856..5380
                     /locus_tag="ACG816_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4919501.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5396..6895
                     /locus_tag="ACG816_000589"
     CDS             5396..6895
                     /locus_tag="ACG816_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4919502.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6983..7585)
                     /locus_tag="ACG816_000590"
     CDS             complement(6983..7585)
                     /locus_tag="ACG816_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4919503.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7689..8645
                     /locus_tag="ACG816_000591"
     CDS             7689..8645
                     /locus_tag="ACG816_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4919504.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8950..9906)
                     /locus_tag="ACG816_000592"
     CDS             complement(8950..9906)
                     /locus_tag="ACG816_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4919505.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10080..10640
                     /locus_tag="ACG816_000593"
     CDS             10080..10640
                     /locus_tag="ACG816_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919506.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10633..11457
                     /locus_tag="ACG816_000594"
     CDS             10633..11457
                     /locus_tag="ACG816_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4919507.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11467..12084
                     /locus_tag="ACG816_000595"
     CDS             11467..12084
                     /locus_tag="ACG816_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4919508.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12102..12785
                     /locus_tag="ACG816_000596"
     CDS             12102..12785
                     /locus_tag="ACG816_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4919509.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13468..14751
                     /locus_tag="ACG816_000597"
     CDS             13468..14751
                     /locus_tag="ACG816_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4919510.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14767..15654
                     /locus_tag="ACG816_000598"
     CDS             14767..15654
                     /locus_tag="ACG816_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4919511.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15671..16507
                     /locus_tag="ACG816_000599"
     CDS             15671..16507
                     /locus_tag="ACG816_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4919512.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16510..17133
                     /locus_tag="ACG816_000600"
     CDS             16510..17133
                     /locus_tag="ACG816_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919513.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17130..18422
                     /locus_tag="ACG816_000601"
     CDS             17130..18422
                     /locus_tag="ACG816_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4919514.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18781..18878
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18994..19575
                     /locus_tag="ACG816_000602"
     CDS             18994..19575
                     /locus_tag="ACG816_000602"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4919515.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19578..20885
                     /locus_tag="ACG816_000603"
     CDS             19578..20885
                     /locus_tag="ACG816_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4919516.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21301..21789
                     /gene="purE"
                     /locus_tag="ACG816_000604"
     CDS             21301..21789
                     /gene="purE"
                     /locus_tag="ACG816_000604"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4919517.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNSLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21782..22906
                     /gene="purK"
                     /locus_tag="ACG816_000605"
     CDS             21782..22906
                     /gene="purK"
                     /locus_tag="ACG816_000605"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4919518.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22919..24214
                     /gene="purB"
                     /locus_tag="ACG816_000606"
     CDS             22919..24214
                     /gene="purB"
                     /locus_tag="ACG816_000606"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4919519.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="ACG816_000607"
     CDS             complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="ACG816_000607"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4919520.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26293..26670)
                     /locus_tag="ACG816_000608"
     CDS             complement(26293..26670)
                     /locus_tag="ACG816_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919521.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26926..27654
                     /gene="purC"
                     /locus_tag="ACG816_000609"
     CDS             26926..27654
                     /gene="purC"
                     /locus_tag="ACG816_000609"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4919522.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27654..27908
                     /gene="purS"
                     /locus_tag="ACG816_000610"
     CDS             27654..27908
                     /gene="purS"
                     /locus_tag="ACG816_000610"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4919523.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIAKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27910..28581
                     /gene="purQ"
                     /locus_tag="ACG816_000611"
     CDS             27910..28581
                     /gene="purQ"
                     /locus_tag="ACG816_000611"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4919524.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28582..30804
                     /gene="purL"
                     /locus_tag="ACG816_000612"
     CDS             28582..30804
                     /gene="purL"
                     /locus_tag="ACG816_000612"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4919525.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30789..32228
                     /gene="purF"
                     /locus_tag="ACG816_000613"
     CDS             30789..32228
                     /gene="purF"
                     /locus_tag="ACG816_000613"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4919526.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32251..33303
                     /gene="purM"
                     /locus_tag="ACG816_000614"
     CDS             32251..33303
                     /gene="purM"
                     /locus_tag="ACG816_000614"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4919527.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33300..33878
                     /gene="purN"
                     /locus_tag="ACG816_000615"
     CDS             33300..33878
                     /gene="purN"
                     /locus_tag="ACG816_000615"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4919528.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33875..35410
                     /gene="purH"
                     /locus_tag="ACG816_000616"
     CDS             33875..35410
                     /gene="purH"
                     /locus_tag="ACG816_000616"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4919529.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35426..36700
                     /gene="purD"
                     /locus_tag="ACG816_000617"
     CDS             35426..36700
                     /gene="purD"
                     /locus_tag="ACG816_000617"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4919530.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36822..37736
                     /locus_tag="ACG816_000618"
     CDS             36822..37736
                     /locus_tag="ACG816_000618"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4919531.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37869..39434)
                     /locus_tag="ACG816_000619"
     CDS             complement(37869..39434)
                     /locus_tag="ACG816_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919532.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39427..40878)
                     /locus_tag="ACG816_000620"
     CDS             complement(39427..40878)
                     /locus_tag="ACG816_000620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4919533.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40892..41908)
                     /locus_tag="ACG816_000621"
     CDS             complement(40892..41908)
                     /locus_tag="ACG816_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4919534.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42128..43216
                     /gene="chvE"
                     /locus_tag="ACG816_000622"
     CDS             42128..43216
                     /gene="chvE"
                     /locus_tag="ACG816_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4919535.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43270..44796
                     /gene="mmsA"
                     /locus_tag="ACG816_000623"
     CDS             43270..44796
                     /gene="mmsA"
                     /locus_tag="ACG816_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4919536.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44798..46003
                     /gene="mmsB"
                     /locus_tag="ACG816_000624"
     CDS             44798..46003
                     /gene="mmsB"
                     /locus_tag="ACG816_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4919537.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46260..47237
                     /gene="guaC"
                     /locus_tag="ACG816_000625"
     CDS             46260..47237
                     /gene="guaC"
                     /locus_tag="ACG816_000625"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4919538.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47501..48925
                     /locus_tag="ACG816_000626"
     CDS             47501..48925
                     /locus_tag="ACG816_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4919539.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(48967..49224)
                     /locus_tag="ACG816_000627"
     CDS             complement(48967..49224)
                     /locus_tag="ACG816_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919540.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            49386..50495
                     /gene="pdhA"
                     /locus_tag="ACG816_000628"
     CDS             49386..50495
                     /gene="pdhA"
                     /locus_tag="ACG816_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4919541.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50498..51475
                     /locus_tag="ACG816_000629"
     CDS             50498..51475
                     /locus_tag="ACG816_000629"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4919542.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        16257 a         8999 c        11271 g        14992 t
ORIGIN      
        1 aatcacttta cataagtttc aaaagtttct ttaatttcct attgttcaat aagtaacaat
       61 gtaaagataa aattaaaaat gttaatacgt attagcaaaa taaaagcttt ttttattgtg
      121 aataaatgaa cataattagg aggagaaaga tgaacttatt tgacattttt cgatcgactt
      181 tgacagatat gtcgattgtc agtgcaatca cttcaacagt ttttattata ttattagggt
      241 ttgtttgtag aaaaaagggg attttttctg aatctgttgg aaaaattcta tcgaaagtcg
      301 tcttaacagt ggctttgcca gcactggcat ttcgagcttt catgcaagat atcaatacag
      361 aattactgat gcaaggaatc aatgtcttga tttggggatt agttatttat attattctta
      421 ttctagtttc aaaacctttt ttcttaagct ataaagggga taagcaaaca acgttgcgtg
      481 tcctgacgat ctttggttct acgacctttt tcggaacgcc tatcgtcagt gcgatttatg
      541 gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc tttttgtatt
      601 cttatggata cataaaaatg agtggcttga agatggaagc taaaaatatc aaagcgatgt
      661 tcgtcaatcc aatcattatc gctacttttg ccggcctttt catttggctg ttccaagggt
      721 atctgccaca agtgtcagtt acaggtgcag aaggagagat aagccaagta gcgtttttgc
      781 gaattgacca gactgcaccg tggctatttc agccgatgga ttatttagct ggtttaagtt
      841 caccattggc gtggctagca atcggcgcaa cattaggtga agtaagcttc aaaaaagctg
      901 catctgaaaa aacttcatgg tattacagtc tagtcaaagt ggtatttgta cctgcattga
      961 atatcgtgat tctagcagtt ttgacggcaa cgaatattct accagtcaat tttgaagcac
     1021 tggcaacaat cgttatcatg atggctacac cagctgcaac agtggcagca gcctatgcta
     1081 tcagttttga tagggaggca cttttagcct ccaatgcatc cttactgtca actatttttg
     1141 ctgtggtact gacacctgta tggatcgttg tattacaaat gattcatggc ataggtttat
     1201 tttcataaga aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt cgtccgaacg
     1261 agatagattt ttttgaaaaa ttgaatattt accattatga tctcagtctt tatgaagaac
     1321 tgttgacaca tgacaatatc gtgtcagcaa aggaccatga tgctgtattg ttgaggggaa
     1381 attgtatagc agatgagacc aatcttgcaa aaatgcagga atatgggatt cgttatgttt
     1441 ttacacggac tgtgggagtc aatcatatcg atctacaagc ggcagctgat tttggtatga
     1501 cagtagcccg tgtaccatcg tattcgccaa atgcgatagc agagttatct ttgacttttg
     1561 ccatgatgct tttgagaaat actgcgtata cgacgatccg aacatctttc aaagattttc
     1621 gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg atcataggaa
     1681 caggcaggat agggttgacg gaagctgttt tgtttaaggg actaggagcc aaagtgatcg
     1741 gctacgatat tcatcagaca gaagctgcta aaaaagtatt gacgttccac tcgcttgaag
     1801 agttattagc caaaagcgat attgtcagta ttcatgtacc tcatatccca ggtgaaaacg
     1861 atcaaatgat caatgaagga tttttagctc aaatgaaaaa aggtagcatc ttgattaata
     1921 cagcccgtgg agagcttcaa gataatgaag ctattttaag agctttgaaa agcaatcatt
     1981 tagctggttt tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc cgatccttca
     2041 agccatggga gacgattcca gaccctgcta tccagcaatt agtagaaatg taccctagag
     2101 ttcttgtaac cccgcatgta ggttcaaata cagatgaggc attatcaaat atgatttcga
     2161 caagtttcga gaactttcgt gaaataatag aaactggaaa aacgaaaaat gaggtatctc
     2221 ttcctaaagc ccgtcaatta agataagaaa acatggatat tcgtcaataa aatgtttaaa
     2281 aatagtccaa gtaaacagtt gttacttctg tatacttgga ctattttgta tgggataatt
     2341 cgatgactca acagtaatct gtgagctttt tccgcccatt ctttgtcaat tcataacagg
     2401 ttagcggccg gccgatttcc aaatatttga ttctttcgtt caatatatct tgttctacta
     2461 aaaaattcaa atatttcttg acggatatac gtgacagtga gcttttttga gccagttctt
     2521 ttgttgaaaa aatagtatct tcttttagaa taaatgcact tatttttttt aaagtcagtt
     2581 ttgacagacc tttaggcaaa tcgatgaatg atggtatttc gtgaatggga gtggtgtggt
     2641 tgaatacttg atcgatcaat ttctgatcgg ctttttcagt cgtttggatt gtctgagaga
     2701 aagcaagata cttttcgatc gccagctgaa aacgttgcaa agtgaacggt ttgatcaaat
     2761 aatctaaggc accgtagcgg atcacattct ttatggtttc gatatcatct gcagcagtga
     2821 tcaaaatcac tgggatatca tttccttgct gtctaagcca agataaaaaa tggataccac
     2881 tttcgccagg catgtataca tcaagcagca gcagatctgc gtttttttgt ctgaaatatt
     2941 tttttccttc ttctacattt ctgaaacttt cgattacgtt catattaggg atgctttcca
     3001 caaaagtacg attcaattct gcaaccattg gatcatcttc gattatcagg acatttgtca
     3061 tcattttgat cttccttttt tggtaattct agatagacga ttgtaccgcc atttgtttgt
     3121 ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg aatggatgag attcaatcca
     3181 aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa atattttttc tgagatttct
     3241 tctgatatcc cgacaccgtc atcgcagacg gtcacgatga atacttcagc ttctgtgtca
     3301 tagtttaaat gaatccggac ctgtccgttt ctttcatgtt tttgtagcac ggcttctttt
     3361 gcattatcaa gtaagttccc taacacttgc aaaagtgaat ggatggagtc atccatctcc
     3421 attttaggga taaaagaatc gctttctaac agcatttcaa cagattgttc tttggcttcg
     3481 tttattttgc ctaataaaaa accggcaatt gcaggtactt tgatcagact tgagacttgg
     3541 ccaatctctt ttttataatt ttgatttagc tgctggataa aatgggtgac ttcttcatac
     3601 ttcttcaaat caatcaaacc agaaatgaca tgaagtttat tcatgaattc atgggtttga
     3661 gctctcatgg agtttatata ttgtttcgtc cccccaagct cctccattaa tttcattact
     3721 tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct aaaaagaggt
     3781 gtaacgctag aaatagtttc tatcccattt aaatacatgg attgagtccg aacggtcgtt
     3841 tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat tatcactgat ttttttccta
     3901 cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt gtattgatca
     3961 agaggatagt tttatcagaa gagatggcta agattccttc atctacttca ttttcgataa
     4021 tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc agtacttttt
     4081 tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca ataatcaatg
     4141 aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg tttgttaagc
     4201 caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa tagcggaagc
     4261 cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc acttttttca
     4321 catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga gaatatcgaa
     4381 tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg atttcatttg
     4441 ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg atgagtttat
     4501 cattggcaac gattttagca atgctagaga ttttatctga cacactagat cggttcatcg
     4561 aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat gtggagaaac
     4621 aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa aaaggaaaca
     4681 taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa atttaacaaa
     4741 ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta aaagaagcta
     4801 tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg tgtatatgat
     4861 agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg caggattggc
     4921 agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg atccgatgct
     4981 tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc gtttaacaga
     5041 tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt tggtattttt
     5101 gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt cgtcttctga
     5161 aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac gaggtatggg
     5221 agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt tttacagccc
     5281 ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca aggaaagaat
     5341 ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt gttaaatgga
     5401 aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga tgttgctgat
     5461 cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca tcagtggaag
     5521 ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca aaattgtgcg
     5581 tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca gtctattatc
     5641 gagtaaattg gacgtctcca cccctgaagt gtatacggaa caaggcgtac cggtgatgtg
     5701 tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta cagcagcaga
     5761 acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag tattagaagg
     5821 acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa atcgcgataa
     5881 gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg ggttgatcat
     5941 cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact ctttagggaa
     6001 accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag ctctaaaaga
     6061 aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat tacagcgaca
     6121 aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct acaaacaaga
     6181 acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg cccgagcaca
     6241 acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgtcaaa aacaaatcga
     6301 actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag tgaagaaaaa
     6361 agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag tcaaagaagt
     6421 gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg aagccaataa
     6481 gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa aagcagaagc
     6541 cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag ctgtcttgag
     6601 tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc taggaaatat
     6661 cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg gagcgaaccg
     6721 agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa aagcgacaac
     6781 aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta ctagccaagt
     6841 aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag aataaagtag
     6901 tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc tctccatttt
     6961 tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt tggatcttag
     7021 cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt tcgaccaaat
     7081 cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa ttgataccga
     7141 ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac aaagcggatt
     7201 cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa atgataccgg
     7261 aattattgtt aataccatct gacacgacga tcacaataat aactagaata ggaagcaaga
     7321 tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa atgatagcta
     7381 atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg gttgtagtca
     7441 agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt cctcgttgag
     7501 tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc gtacgcatac
     7561 ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt actatcatac
     7621 ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa taaaaaaggg
     7681 ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc ttcttttcga
     7741 tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc attgcattat
     7801 taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga atcgcagcct
     7861 ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat actatttttg
     7921 gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa ttaaccgaag
     7981 ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac atgtcttggc
     8041 agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct ttgcttgcaa
     8101 atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag atcgaaaaag
     8161 aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat aaagaagatg
     8221 tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa gatgccagag
     8281 aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac ttctctatga
     8341 gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga atccagcaac
     8401 cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa acattttcag
     8461 agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta ttgtatcggc
     8521 aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg ttccagtttt
     8581 tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa aaaatccaaa
     8641 aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac aacctcttct
     8701 ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa caaacatgag
     8761 aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct cacaacctct
     8821 ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa acgaaaaata
     8881 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac
     8941 cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt cgatgacatc
     9001 ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta ccatattctt
     9061 tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct ttgtaccatc
     9121 tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca cataaccgtt
     9181 ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc tgacagcagc
     9241 aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc ctgtttcaaa
     9301 aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac gagcgaaaga
     9361 tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat tgaagaaccg
     9421 gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt ttaaagcccc
     9481 cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt tgaagattgt
     9541 catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt cagccagttc
     9601 ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt ccaatgctgc
     9661 caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt cgatttcagc
     9721 atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac ggtgagcgaa
     9781 gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg tctcttgatt
     9841 gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc attgatattt
     9901 tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct attattataa
     9961 caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat gaaagcagcg
    10021 atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag gggataaacg
    10081 tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt gaaactgaaa
    10141 aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa tggaaagatg
    10201 gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc actgattatg
    10261 aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa aaacgaaacg
    10321 ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg cttagcagca
    10381 aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt agataccttg
    10441 acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg aattttcgtc
    10501 tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc gattgggtga
    10561 atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa gaacaacgaa
    10621 aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat gatcgcagca
    10681 atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa tcaagcagtc
    10741 gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac aaccagcagc
    10801 aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga tgttaaaaca
    10861 gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga aattgatgag
    10921 tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat tgctccagaa
    10981 tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat ggtctcagct
    11041 taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga tgtgatgagt
    11101 cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat tacggagcca
    11161 ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa ctggtacaac
    11221 agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc taaatggatc
    11281 gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca cgaagatata
    11341 actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa cgctgagtat
    11401 atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga aaagtaggaa
    11461 ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg ttggccagca
    11521 gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa gaatctgtct
    11581 tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca atttattgaa
    11641 cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc tagtatcatt
    11701 ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag caaatacaat
    11761 aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga aacaaaggaa
    11821 tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga ttcatgggaa
    11881 cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa tcctgcgctg
    11941 tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt gcaagatgca
    12001 ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga gacgtatcaa
    12061 ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa caatttacgc
    12121 cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc attttagaag
    12181 caagcgggat caagatattt ggaaaattga tccgttcgat catctttact acggatattg
    12241 cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt acacctcatc
    12301 cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc ggtgtaggtg
    12361 gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa gcgcaaggtt
    12421 cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa gtttgtcgag
    12481 tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata gatgaaaagt
    12541 tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca gaagcagtac
    12601 gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt ccaacggaag
    12661 aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg ccaccaagta
    12721 gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa aagaatcatg
    12781 aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc tcgtcttttg
    12841 tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt ttttttacct
    12901 gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa caaaaaaatg
    12961 tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta aaaaaggcac
    13021 ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga actgaccaat
    13081 caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat ttcatacaga
    13141 attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca tgcttattta
    13201 gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt aattatcctc
    13261 ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact atctaatttt
    13321 tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct tggtttttat
    13381 tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt atattgaaaa
    13441 ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat cttagcttta
    13501 agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa aatcacgctg
    13561 aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt aatcgagctt
    13621 tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta tgaaggatat
    13681 tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt ccaactgaac
    13741 aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt ggagcaaggg
    13801 ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta cgacgggcaa
    13861 ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa aaatatattt
    13921 gaacaattaa atatccctga accagcttca aattggacat gggatgaatt tgaacaaatc
    13981 agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga agattttata
    14041 gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta taatcgggaa
    14101 gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt tttccagctg
    14161 cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc tgcaagtacg
    14221 acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt tctatcaagt
    14281 aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca gttgattaat
    14341 ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc gcaaatgctg
    14401 agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga tttctttcaa
    14461 aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat catgtcgaat
    14521 attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt tgattattta
    14581 gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc tttgaatccg
    14641 gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga agtaacagca
    14701 gaagaagcag cagagagtct ttatgagttt gcgtcaaaca ttctaggata gcagcaggag
    14761 gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc aagacaatgt ggtcggttac
    14821 gtatttgctt ctccattcat cctaggattt ctattgataa cagtgatacc gatgatcttg
    14881 tcactttatt attcgttcac cgattatcgt ttgggaaatc cagtctcatg gatcggcttg
    14941 gataattata taaagatgct gagtgatgcc cgttttttga actccatccg agtcactttt
    15001 ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg gtctattgat tgcttatctt
    15061 ttaacgagga atatcaaagg aacgactttc tttcgttctt tgtattatgt gcctagtttg
    15121 atcggtggca gtatttctat cgctctagtt tggcgggagt tgttttctaa aaacgggtta
    15181 gtgaatattt tcctggaacg attaggcgca cctgcgcttt cctggtttag cgatcaaaaa
    15241 ctggtcatga tccctcttgt tttgatgtct gcttggcaat tcggttcggc aatgatcatc
    15301 tttgctgcag gtatcaaaga gatcccaggc tcgtattatg aagcggcaga gatcgatgga
    15361 gcgaacaaat ggcagacgtt ttacaagatc acgttacctc ttctttcgcc gatcattttg
    15421 tataacttgg ttatgcagac aatcgcggcg tttatgactt ttacgcaggc atttatcatt
    15481 acccaaggtg gtccgaatga tgccacgaac ttttatgctc tttatgtgta taaccaagct
    15541 ttttctttta attctatggg gtatgctagt gctatttcgt ggatcatgct gttgattatg
    15601 ggagctgtca cctttctgat tttcaaatca tcaaaaaaat gggtcgttgt gtgaatgaga
    15661 ggaggaagaa atgaataata cagtaacgaa atggtttcaa tacggtttta ttttgctatt
    15721 aggtctagtc atgatttatc cggtcttatg gatgatcgca ggttctttta aaacggatca
    15781 agagattatg agtggttcgc tgaatttgat tccagagact tttcaatggg aaaattacgc
    15841 aaaaggatgg gcggggtttg ctggaatttc attttttact ttctttagga attcgtttgt
    15901 catctctgca gtttcgacga tagggacagt tctgagctcg acttgtattg cttacgcatt
    15961 atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc gtcatgttag taacgatgat
    16021 gattcctgca caagtgattt tgattccgca gtttattatt tacaatcggc tgaatttggt
    16081 tgggacgtat gtgccgctga ttttaccaca tttttttgga caagcatttt tcatttacca
    16141 gatcatgcaa ttcatggtca gcatccccaa agagttagat gaatcagcga ttattgacgg
    16201 atgcagcaag tatagtgtct ttaccaaaat tatttttcct ttgctgaaac cttcgatcat
    16261 cacgacgatc atcatccagt tttattggaa atgggacgac ttcatgggac cattgattta
    16321 tttaaacaag ccgagatctt acacggtatc gattgccatc aaattatttg cagacgctgg
    16381 atctagtacg acttacgcga gtatgtttgc gatgtctacg ttgtcgctgc taccggtttt
    16441 tctgattttc ttattcttca ataaatatct tgtccagggg atcagtacaa gcggtctgaa
    16501 gggctgaata tgaaaaagcc aatagaagag atgtttcttt acaagctcaa ctactatgtc
    16561 tgtcggattt ttttaaccag tcttatcagt aatgctcttt tcgctttagt tctaggatgt
    16621 ttgttcactt taggattagt gctgtctgct cgtgttgtct atctgattta ttttttgatg
    16681 atgtatcctt cttattatct atctgtccat tacttggcag aaaaaaaggt atgttggagg
    16741 aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt ttcagctgtt tgtcattatt
    16801 tttgcagttg attggtattt attagtggga aagattggag cttggtatct gattcctttt
    16861 ctattgtgtc tgttgctttt tgtatcccat agttttttta gtacgctaat tgatatctgc
    16921 aaagaagata aagcagatag tcttgtttat ttgatcaaaa ggaatatctg gctgttgtca
    16981 aaaaacgtca aggggtttct gtggtattta tgccttttgt atggactgtt ctggtgtata
    17041 cagcatgatt ttttactcac tgcattactg tatccagggc tgccatttgt cacagtattt
    17101 tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa atatgtacaa gtcggacttg
    17161 gtgggagagc gcgttttttt tatgaggcaa ttgcctctac gtatcatcaa acttgtgaac
    17221 tagtcggttt ttgtgatgta tctcaaacaa ggatggatta tgcaaatcgg atacttgtga
    17281 acaagtatgg acagaaaaaa gtaaagacat gtatgccatc acaatttgag caactactgg
    17341 atgacgtacg gccggctgaa gtcattatta cgtcagtaga tcgaacgcat catgattata
    17401 tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga aaaacctctg actatcgatg
    17461 aggaaaaagc tcaagaaata ataacggtcc aaaaacagac gggaagaagc ctgcgagtca
    17521 cttttaatta tcgttatgca ccgtatcaca cgaagataaa ggaactgata gaaagtgatg
    17581 tgattggtga tgtgttttct atccacttcg aatggctgtt aaatacgcaa cacggggcag
    17641 attatttcag aagatggcat cgaaataagc acaatagcgg cgggcttctt gttcataaat
    17701 ctacccatca ttttgatcta gtgaattttt ggttgaacag ccgtccccaa aaggtatttg
    17761 cattcggtgg attaaacttt tatgggatgc ataatgcgga gaaacgcgga gtagaaatat
    17821 tttacagtcg atgctatgga agcgaactag ctaaagctga tccatttgct atagacctgt
    17881 cagccaatga ggaattgcga gaaatgtatt tagaagcaga aaaagacagt ggatatattc
    17941 gtgaccgcag tgtattttcc gatgatatct ctattgaaga tacactaggc gtaacggtca
    18001 aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc gtatatgccg tgggaaggat
    18061 tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat gaaagtgatc gaaaagtctt
    18121 acatcaatgc aggtggagat aaaaaggatg aaggagcagc cgttcttcag tccatccatg
    18181 tctatccgat gttcggtgag ccatatgagg taatcgtcga acaagaagcg ggcggacatg
    18241 gcggaggaga tccgcgttta ttagatgatc tatttggtga agaaaaagaa gaagatccgt
    18301 ggaatcgggc agcaactcac gtagatggta ttttgtcgat tttgacagga atcgcagcaa
    18361 atcattctat tgcttcagga aaagcagtag tgattgacga gcttgtcagc ttaaaagaat
    18421 aagaatagat ttacggaaat tacttcccgt agtttataaa atcttgcctt attgccgata
    18481 ggctgtcttt tgaacactat ttattcggaa aaaggggcta tgacatgctt tttgtcatgc
    18541 cttcttttta tgtgggctgc ttttttagat aggagaaaat aaaaataact atttaaaccc
    18601 gaacaaaaag gaataatgca ttaaaaatca ttagatttta caaaaaaata agtaaaccac
    18661 gttgtcagtt tcagaaagta gtgttagact gttttcaata tttgaaacaa cattttttat
    18721 tcaaatatgg caaactgcct tcacgtgcaa aacagttgca agaaaatgaa tttacactat
    18781 tattagtttt acatacctac ttatatatcg tcataatatg gatgacagtt tctagccagt
    18841 accgtaaatg ctggactata agtaaaagat tggctatttc acccttcttt tcttatgata
    18901 aagtggaggg tgtttttctt taacagaaac atagtaggtt tttgcacaaa agcggttatt
    18961 tcggtattaa taaatgataa aggagcaaat aaagtgaaag aattagttga acgtattcaa
    19021 aaagacggac gtatattagg cgagggcgta ttaaaagtcg acagcttcgt cactcaccaa
    19081 gtagaccctg tattgatgga acaaatggga aaacggtttg cagaagtatt taaagaacaa
    19141 ggaatcacga aagttgtgac cattgaagct tctgggatcg caccagcttt atttgctgca
    19201 agagaattag atgttccaat gatcttcgct agaaaagcaa aaagtttgac gatggatgaa
    19261 gaattattga cagcttctgt ttattctttt acgaaacaag tgacaagtac gatttctatt
    19321 tctcgtaaat ttttatcttc agaagacaaa gtattgatta tcgacgattt cttggcaaac
    19381 ggacaagcgg caaaaggatt gatcgaatta tgccagcaag ctggagcgca agtagaagga
    19441 atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc agttacttga agaaatggga
    19501 ttgagagtcg tttctcttgc gcgtattgct tccttgacga atggacaagt tgagtttctt
    19561 gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg aaaagctgcg gttttagggc
    19621 tgcagcatct gctagcgatg tatgcaggag cagtagcggt gcctcttttg atcggtacag
    19681 gactaggctt taatcaagaa cagatgactt atttgatttc aattgatatc tttatgtgtg
    19741 gattagcgac attattgcaa ttgacagtta accgattttt cggaattggc ttgcctgtcg
    19801 ttttaggatg tgcgatccaa gcagtagctc cactgatctt gatcggaacc aatgaaggag
    19861 taggagctat ctacggatct attatcgctt ctgggatctt tgttgtgtta gtttctggtg
    19921 tcttttctaa aattaaaaaa ctattccctc ctatagtaac aggaacggtt atcaccgtta
    19981 ttggtttgac tttgattcct gtagcaatcg agaaaatggg tggaggtaat gcaacagcag
    20041 ctgattttgg tgacaaaaca aatctgctac ttgcatttgt gacgattctc ctaattgtag
    20101 gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc ggtattgatt ggtttagtcg
    20161 gaggaagcat cttagctgct ttcatgggaa tgattgatct gagcgcgatt ggagatgcgc
    20221 cgttgttcca tgttccacaa cccttttatt ttggtaaacc aactttcgac gtatggtcga
    20281 ttttactaat gatcatcatt tccatggtca gcatggtaga atcaacaggt gtatattttg
    20341 cacttggtga cattactggg aaaaatgttg gagaagaaga attgaaaaag ggatatcgtg
    20401 cagaaggact tgccgttatc ttaggcggta ttttcaatac attcccttat actggctttt
    20461 ctcaaaatgt cggattggtt caactttcgg gaatcaaaac aagaaaaccc atctatttct
    20521 ctgcattctt cttgattgtt ttaggattat tccctaaaat cggtgcgtta gcacaaatca
    20581 tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt cgggatggtg gcagtccaag
    20641 gaatccgtat gctgttggaa gttgatttca gcaatgataa aaatctattg attgttgctg
    20701 tttctgttgg atttgggctg ggtttcaatg ttacgccgac attattcagt caaatgccgc
    20761 aaacattgca aatgttcact ggaaatggca tcgtaatgag tagcattacg gctatcatat
    20821 tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat attggaagaa gccttcaaga
    20881 agtaatagag caaaagaaag gttgtgaaat cagcgttttg acctgattgt tagagaaaca
    20941 aacggaaaaa tagttcctcg tattttgccg tttgtttgag ctaataccga gggtcagctg
    21001 attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa
    21061 caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt tccttgtgac cgaggagttg
    21121 gcaggagaac accgtttatc cataaaaagg aatgtgacct aacttttgtc acattccttt
    21181 tttttatctt taataaacac gaacaataaa atgtttttct ttgttaacat tcgaaatttc
    21241 tttgacatca aaaaaagtga ttgttaaaat atgaatgtag aaaaactggg aggaaaaaca
    21301 atgacagcaa aaatctctgt gattatgggg agtacttcag attgggaaac aatgaaagaa
    21361 gcatgtcaaa tattggatga attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat
    21421 cgaaccccag atttgatgtt tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc
    21481 attattgcag gagcaggggg agccgcgcat ctgccgggta tggtcgcatc aaaaacaact
    21541 ttgccggtta tcggtgtacc ggtccaatca cgaacattaa atagtttgga ttcgcttctt
    21601 tcgattgtcc aaatgcctgg aggagtacct gtagctacta cagcaatcgg aaaagcggga
    21661 gcaattaatg ctggactact tgcgatacag atgctttcca tgtatgattt agaattggag
    21721 gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa
    21781 cttggttaaa cctttgttgc ctggttcaac gatcgggatc gtcggtggcg gtcaattagg
    21841 tcgtatgatg gcgattagtg caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt
    21901 gagcgattgt ccagccgcac aagttgcaga ttggcacatt gaaagtgtct atgatgacac
    21961 ctttgcgctg aaagagcttg ccagaagaac agatgtcatt acctatgaat tcgaaaacgt
    22021 cagtgtagag gcgttaaatg cgattttacc catgtcattt attcctcaag ggacagactt
    22081 gcttgcgatt acgcaagacc gcctgttaga aaaatctttt ttggaaacga ataacatcgt
    22141 gattgctcct tatgcaacga tcgtcagtcc gacggatatc caagatgcga ttgacgggat
    22201 cggctatcct tgtgttttga aaaccactcg aggcggctat gatggcaagg ggcagtatgt
    22261 tttgaaaagt cgggcggatc tagctcctgc catggacctt ttaagagaag gaacctgtgt
    22321 tttagaagca tggatcccgt ttgaaaaaga gatttcgatc atggtggcag gaaatggtca
    22381 aggagatttc acgacttttc cggtcgtgga aaacatccac cacaacaata ttcttcatga
    22441 aacgattgct ccagccgcga ttgatcagga tgtgatcgag gaggctgagc gtattgcccg
    22501 tgtgattgca gaagcagtga gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa
    22561 aacaggcggg ctatatgtga atgaattagc tcctcgtcct cacaattctg gacattattc
    22621 tattgaagct tgttctatga gtcagttcga tgcccatatt cgaggtattt gtggttggcc
    22681 attaggagaa aaagtacgtt tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca
    22741 actgctggaa agctatcgct tgattgagaa taagccagac tggcaattcc atttctatgg
    22801 aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc actatcttga cagaagacat
    22861 ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg gattagagga gaaaaaacat
    22921 gttagaacgt tatacacgcc ctgaaatggg gaatatctgg acggaagaga acaaataccg
    22981 tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc
    23041 gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt gatatcgatc ggatcttgga
    23101 aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt
    23161 gggagaagaa agaaaatggg tccattatgg tctaacaagt acggatgtcg tagatactgc
    23221 ttatggctat ctcatcaagc aagcaaatga tattttacgg gaagacttga aacgttttac
    23281 ggaaatcatt ggcgaaaaag caaaagaaca caaatataca gtgatgatgg gtcggacaca
    23341 cggtgtccat gctgaaccaa ctacttttgg attgaaactt gccacatggt attcagaaat
    23401 gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc
    23461 aggagcagtg ggaacgttcg caaatatccc gccatttatt gaagagtatg tatgtaaaca
    23521 attggggatc cgtccacaag aaatctctac tcaagttctc cctcgggatc tacatgctga
    23581 atatttctca gcgatggcgc ttattgctac aagtattgaa cgttttgcaa cagagatacg
    23641 cggattgcaa aaatccgaaa cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg
    23701 ttcttctgct atgccgcaca aaaggaatcc gatcggttca gaaaatatgg ctggtctagc
    23761 cagagtggtg cgcggacaca tggtcactgc ctatgaaaat gtcacattat ggcatgaaag
    23821 agatatttct cattcttcag cagaacgaat catcttgcct gataccacga ttttattgaa
    23881 ttacatgtta aatcgttttg gcaatatcgt taaaaatcta acggtctttc ctgaaaacat
    23941 gaagcggaat atgtctgcaa catttggttt gatttatagc cagcgcgtta tgctgaaatt
    24001 gatcgataag ggaatgacaa gagaagaagc ttatgatctt gttcagccaa aaactgcgta
    24061 cgcttgggac aaccaagtgc agttccgccc gctgttagaa gaagatccaa ccatcacatc
    24121 ggtattatct aaagaagaaa tagacgatgc ctttgattat catcatcatt tgaaaaacgt
    24181 tgacgtgata ttcaaacgag taggtttgga ataaagaaga gtgagttgca aaattcaatt
    24241 aaaatagaat agcaaaaata atcgagtaga ggggaaagcc aactgacctt ccgcccctct
    24301 cacaccaccg tacgtacgga cccgtatacg gcggttcaat aagttgagtg tacgaactgg
    24361 tacttcaagg agatgtcctc atatccttgg cgggccagtt ctttgtctgt taatgatcga
    24421 tgaagaatat gacttttgga gactgaccaa ataccttttc ttgtatttga ccattcatac
    24481 gctttcgctt tatcgatccc taaacgaatc agattttttc ttttggttct tggattcttc
    24541 cattgtttcc aaatatattg tctgattctt cgcttcaacc aaccgtttaa ctccctcatg
    24601 aattctttca tctcaccaat tccatagtag ttgatccaac cagtcattag ttgcttgatt
    24661 tcttttaaaa taagttggat acttcttcca cggtttcttt tagtgagttg tttgagcttg
    24721 cttttcacac gctgttttgc ttttgcgtga gggcgaattt taataccttt ctttgttggc
    24781 agaatgcaga atccaagaaa ctttcgtttc aatggacttc ctaccgcact cttttctcga
    24841 tttaccgtca actttagttg ttcttcgaga aattgaatgg atttcttcat cacacgctgt
    24901 cctgctcgtt tgcttctaac atagatattg cagtcatcgg cgtatcgaac aaatttatgt
    24961 cctagatttt ctagcacttg atcaaattcg ttcaggtaga tgttacttaa caggggagag
    25021 agattgcctc cttggggcgt tccttcttca gttgttgtga tgagtccatt ttccattaca
    25081 ccactcatta agtattgacg gatcagtttt aggacttgtt tgtcctttac tttccgctca
    25141 atgaaataca tcagcttatc gtgatttacg gtgtcaaagt aggctttcat atcaatgtcc
    25201 accacatatc ggtaaccttg ttcataataa gattttgctt tcttcactgc gtcatgtgcg
    25261 ctgcgatttg ggcgaaaccc ataactatta tctgagaatt ctggttcaaa tagcggttgt
    25321 aacacttgat tgattgcctg ttgaagcata cgatcaataa ccgtgggaat tcctagtttt
    25381 cttacaccgc cgtttggttt cggtatttct actcgcaaaa caggctgagg tttatagttc
    25441 ccttggcgta aattggatag taattcctcg cggtgctctt tcaagtaagg aagcagttga
    25501 tcgacggtca tcccatctgc accagccgcc cctttatttc tgactacttg aagatacgct
    25561 tgattgagat tattccgatc tagaattctc ccaaacagat tttctacacc atcttgtttc
    25621 ctctgttcac cgcaagaagt gctgcgcact tcatcatacc cttcgtgttc cacactagct
    25681 ctttgatgat agccaagttt tcttgttttc tgcggtcgtc gcatcttctc acacctccta
    25741 aattattcaa gattcttatt gttcagtcct tcgtaacggt cagtgtcgtt actactatga
    25801 cctcggctga cttctcatga ttcaccatca tgtcgccatg atcgttgtgc tggtttgttt
    25861 tcattcaacg cccacaacac cctcatgaga tctcccccgg taagagtgaa aactttcctc
    25921 ccatgtaact gctagattta ctgtataggt ttcgggcagt attggacttt gtcttgtgat
    25981 gaagacttat ccgacctaat tcagccttgt atctagtttc tgttcgtcag ttcaggagtt
    26041 tgcgtccgac ttccttcagc cacaacctca cgattgccgc cttgtctttc gctaacagtt
    26101 cctactgcca agcctgtagt ggactttcac caccaagttt tcacccatgc agggcgcaca
    26161 ccaaaaaaga agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat
    26221 caataatagg aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa
    26281 aaaatagagg aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag
    26341 atgttgcagc aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg
    26401 gatacgataa tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc
    26461 tttgtgttct gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc
    26521 tcggcggatt tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt
    26581 gatcgacagc aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa
    26641 aagctgacct tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca
    26701 aatttttagt taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt
    26761 ataagatttt cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac
    26821 atatttttgt tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa
    26881 aaacgatgta tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa
    26941 cttttgtatg aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg
    27001 atagaatatt tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga
    27061 aaaggagaat tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga
    27121 ataaaaaatc attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa
    27181 attatccctc tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta
    27241 ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac
    27301 cagttggatg atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg
    27361 gaagaaatca gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta
    27421 tttgctaagg tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga
    27481 caaattttgc ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg
    27541 aatgaacata tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat
    27601 caagaagtat ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt
    27661 tcgtaaaagt atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa
    27721 aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga tatccgaata gcaaaatact
    27781 ttgagataca acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg
    27841 acaagctgct tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg
    27901 aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg
    27961 ttatgggcag ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc
    28021 tctttagaag gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg
    28081 agatgcggtg cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa
    28141 gaagggaaac cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt
    28201 ttgccaggtg tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg
    28261 aaagtcaacc ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt
    28321 ccagttgctc atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa
    28381 aaaaataatc agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt
    28441 gcagggatta cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca
    28501 gtagaagact tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac
    28561 ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag
    28621 aaaaaaagaa tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa
    28681 gatatcttag gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc
    28741 gaacattgtt cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct
    28801 catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca
    28861 gttgtcttca aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct
    28921 gcgacaggag ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg
    28981 ttgctagatt cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa
    29041 gaagtagttg caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc
    29101 gaaattgcat ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt
    29161 ttgattgaac acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg
    29221 tatgtaggtg caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa
    29281 tttaatcaag aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa
    29341 aagctgctgc ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt
    29401 atacaggata tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct
    29461 ggctcaggac ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct
    29521 tatgaaatga tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag
    29581 gaagaaaaaa tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa
    29641 gtaacagacg acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca
    29701 gtcgatgcat tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga
    29761 atcgtagaat ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta
    29821 ttgcttgatc tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat
    29881 gattcacaag tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg
    29941 gttagaggga cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat
    30001 ttggatccaa aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct
    30061 agcggtggta agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca
    30121 gaagtttttt gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta
    30181 ggaactcctg ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc
    30241 tatccaacac caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact
    30301 caggaattca aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt
    30361 gatggcagtg aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt
    30421 gatctatcca ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg
    30481 attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct
    30541 ttcaaacatc agctagggat atctgtacag tttgaattgt cctctgctca attatttgca
    30601 gaaactcaat caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa
    30661 atgatgggag atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt
    30721 tcagcaactg acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat
    30781 gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt
    30841 ggggtcatcc agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag
    30901 gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt
    30961 tggggctgct ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag
    31021 ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc
    31081 cttttctatt caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca
    31141 atgctcgttc ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg
    31201 atacggagat attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat
    31261 taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg
    31321 caatgattgc cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa
    31381 acggagcata cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta
    31441 taagagatgt ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt
    31501 gctttacgaa ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac
    31561 cagattcaaa catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt
    31621 tagcgaaaga atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt
    31681 cagccgcaag cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa
    31741 atcaatacat tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc
    31801 gaatgaaatt atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg
    31861 attcaattgt tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg
    31921 caaaagaagt ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga
    31981 ttgatattca aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag
    32041 aatgtatcgg tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg
    32101 gtctggaaaa agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc
    32161 cgacgccgtt atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt
    32221 tcttttaaaa gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa
    32281 gctggcgtag atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa
    32341 aaaacggaac gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta
    32401 tcggtctttc aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa
    32461 ttagcagtgg caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg
    32521 tgtgtcaatg atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca
    32581 acggggaaaa atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt
    32641 gttcaaggaa atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca
    32701 ggtaatgatt atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg
    32761 acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat
    32821 tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca
    32881 tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc
    32941 tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc
    33001 actggtggag ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa
    33061 atcaaacttg gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa
    33121 attcctgaga tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc
    33181 caagaatcga aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat
    33241 gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga
    33301 tgagaatcgc tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc
    33361 tatcgaaaaa aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat
    33421 atgttttgaa acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat
    33481 ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg
    33541 atttgatcgt attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg
    33601 ataaaagaat catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca
    33661 tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc
    33721 aaggtgtaga tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca
    33781 cgttcgattc tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga
    33841 ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc
    33901 agataaaact gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat
    33961 ttcaacagga ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga
    34021 agaagtgaca gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat
    34081 ccatggtggt cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga
    34141 aaatatccaa ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat
    34201 gaaaccagat gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat
    34261 gctccgtagt gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta
    34321 tgaagaagta ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa
    34381 actagcggca aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt
    34441 aacggattgg gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca
    34501 aacgcttcgt tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct
    34561 ggaaaaatat tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa
    34621 tatccgagat gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt
    34681 cgccttgaaa cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg
    34741 ggaagcagcg tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg
    34801 agaagtagat ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc
    34861 cccttcttat acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact
    34921 gacagtagac tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg
    34981 tggacttctt gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt
    35041 gacaaaacgt aagccgactc cagcagaagc aaaagcatta gcgtttgctt ggagagctgt
    35101 caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg
    35161 agccgggcag atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa
    35221 aatggaaggt gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata
    35281 tgcagccaaa catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga
    35341 ttctattgat atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt
    35401 tagacattag agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga
    35461 acatgcaata agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc
    35521 aggaaatccg gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca
    35581 ttcagcgtta atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga
    35641 agttcctttg ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg
    35701 tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga
    35761 tacttatcaa atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc
    35821 gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa
    35881 aggtgtagtc gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa
    35941 ccagcatcga tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga
    36001 agaattctcc cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca
    36061 agatcataag cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta
    36121 ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa
    36181 accagcggct ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg
    36241 tttgattgct acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc
    36301 tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt
    36361 attagagaat cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt
    36421 ggttgcagct gccggttacc ccgaagaata tgagacagga atggttctac cagattttct
    36481 ggaagaagag ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg
    36541 agcaggagga cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa
    36601 aaaaatttac accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat
    36661 tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata
    36721 aaaaatacaa caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg
    36781 tggtacactt ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag
    36841 gtacttctga tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg
    36901 aagatccagt aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg
    36961 cagatattta tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat
    37021 caatcaaacg agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt
    37081 ttgatttttc taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa
    37141 tggattatgt ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag
    37201 tagctgaagc gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca
    37261 atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta
    37321 atcagctgca atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata
    37381 tgacagatga agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg
    37441 atatgacgat ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg
    37501 ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa
    37561 cgacaccaac tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga
    37621 tcgccggtac gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata
    37681 tgtctcggga agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat
    37741 tgtcataagc aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt
    37801 tcagcttcga gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat
    37861 ttcgtttatt agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa
    37921 atcccttgtt ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca
    37981 aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt
    38041 ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg
    38101 aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg
    38161 gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt
    38221 gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa
    38281 agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct
    38341 tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa
    38401 cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta
    38461 gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa
    38521 tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg
    38581 gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt
    38641 ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca
    38701 tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca
    38761 actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc
    38821 ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact
    38881 tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca
    38941 gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga
    39001 ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct
    39061 aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc
    39121 agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca
    39181 ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac
    39241 ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt
    39301 gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa
    39361 ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata
    39421 aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt
    39481 gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta
    39541 ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc
    39601 gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc
    39661 gttgtctata actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa
    39721 tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa
    39781 gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag
    39841 catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt
    39901 ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc
    39961 atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc
    40021 caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg
    40081 tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat
    40141 tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa
    40201 gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg
    40261 gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac
    40321 tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct
    40381 tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac
    40441 agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt
    40501 attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa
    40561 cgtactaatt tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag
    40621 ttcttctagg atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc
    40681 ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat
    40741 atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag
    40801 caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat
    40861 gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta
    40921 tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg
    40981 tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt
    41041 tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc
    41101 catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag
    41161 ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat
    41221 catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga
    41281 caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg
    41341 ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt
    41401 tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat
    41461 tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga
    41521 cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg
    41581 ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga
    41641 tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca
    41701 tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt
    41761 cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg
    41821 ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa
    41881 gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact
    41941 atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga
    42001 aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa
    42061 aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg
    42121 ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag
    42181 cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg
    42241 aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc
    42301 ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa
    42361 tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc
    42421 tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt
    42481 gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga
    42541 taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga
    42601 aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact
    42661 aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt
    42721 cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac
    42781 ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc
    42841 tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg
    42901 gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg
    42961 cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt
    43021 agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa
    43081 tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt
    43141 atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc
    43201 cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg
    43261 ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga
    43321 atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc
    43381 ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat
    43441 ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa
    43501 gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt
    43561 tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg
    43621 gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg
    43681 aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca
    43741 ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa
    43801 gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc
    43861 attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg
    43921 gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc
    43981 ggaatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa
    44041 gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata
    44101 aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg
    44161 ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata
    44221 tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt
    44281 gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg
    44341 gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta
    44401 gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca
    44461 aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta
    44521 tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata
    44581 gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa
    44641 gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat
    44701 acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc
    44761 ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac
    44821 ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta
    44881 cagcatggtg atcattttag cgggactact tattgcgttt caattgatga cggatggtat
    44941 tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata tattagtact
    45001 agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt
    45061 gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg
    45121 gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg
    45181 ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg
    45241 tggactgaca caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca
    45301 aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac
    45361 attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa
    45421 tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt
    45481 atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc
    45541 gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac
    45601 agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg
    45661 gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt
    45721 agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat
    45781 ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg
    45841 aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc
    45901 gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc
    45961 cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc
    46021 agaaaaaaga ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc
    46081 attgtttcgt ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct
    46141 ctttttgctc ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc
    46201 cttgattaaa ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa
    46261 tgaaagtatt tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc
    46321 gttctgaatg tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc
    46381 ctgccaacat gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt
    46441 atttctatat catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga
    46501 aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta
    46561 tagagaagtt ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg
    46621 gacatgctaa ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt
    46681 ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg
    46741 cagatgcaac aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag
    46801 gctttgggac tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca
    46861 agccgatcat cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt
    46921 ttggtgcgac aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg
    46981 aaacaaaagt cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc
    47041 aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt
    47101 tgaaagatac actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag
    47161 gaagagatct ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta
    47221 atggagacac aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc
    47281 attaagtaat aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat
    47341 tattatcgca gatacaagct tttgaacatc attattcaga ataagggact gtgataagat
    47401 ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt
    47461 aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa
    47521 aaaacaagga ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg
    47581 aaggatcaat cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt
    47641 gaatcgttcc accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag
    47701 caaacgtggc agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag
    47761 aatcgattgg cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa
    47821 aataagagaa gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat
    47881 gccagatgag cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc
    47941 ggaagaaaaa gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta
    48001 tttggaaagt aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg
    48061 tcagcaagca atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt
    48121 agagtcacct tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt
    48181 tgggataccg atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa
    48241 aaataaattg aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat
    48301 gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat
    48361 tgaagacgat gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg
    48421 aacgattgat ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac
    48481 aacgaaaatc ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca
    48541 agaaatgatg gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa
    48601 tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact
    48661 gataagcgct tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta
    48721 ctatgcctgg attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt
    48781 agatatgggt cttggaattg caccaagtct actttttggg caagatataa atggaatcag
    48841 aatcaattac agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat
    48901 tctagaaaag tggttaacag aataatttca caaagtaaat agattaaaga atataacagc
    48961 ttggatttaa atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac
    49021 taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca
    49081 acaaaactgt ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga
    49141 aaatagaaaa aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa
    49201 tgctgttaat atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct
    49261 tgtatttctc ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt
    49321 ttattcactg tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt
    49381 gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg
    49441 cagatttccc agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag
    49501 tacctgattt atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg
    49561 tattagacca acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa
    49621 ctgctggaca agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc
    49681 ttcttccagg ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag
    49741 cttttttatg gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc
    49801 taccaccaca aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt
    49861 tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac
    49921 aaggtgattt ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt
    49981 atatccaaaa caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa
    50041 cacttgcaca aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc
    50101 cgcttgcagt atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac
    50161 cagtcctaat cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc
    50221 caacccgcta ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc
    50281 gcttccgtaa atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca
    50341 tcgaagcaac taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac
    50401 aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac
    50461 aaattgcaat ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca
    50521 agcaatcaca gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg
    50581 tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt
    50641 cggagaagac cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt
    50701 cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt
    50761 cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac
    50821 tagaaatcta cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt
    50881 gcattcggat aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc
    50941 ttccaaccct tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt
    51001 tgttttccta gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc
    51061 ttacgaagtt cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat
    51121 cacatacgga gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa
    51181 tatatctgtt gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat
    51241 tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg
    51301 cgttgccgcg caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc
    51361 aatcggacga gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg
    51421 gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa
    51481 gaagggacga aacaaagtca tggcttatca atttaaatt
//
LOCUS       DAZUKT010000011        49416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000011 DAZUKT010000000
VERSION     DAZUKT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.011"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..2719
                     /gene="adhE"
                     /locus_tag="ACG816_000630"
                     /gene_synonym="adhC"
     CDS             122..2719
                     /gene="adhE"
                     /locus_tag="ACG816_000630"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4919543.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2948..4054
                     /locus_tag="ACG816_000631"
     CDS             2948..4054
                     /locus_tag="ACG816_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4919544.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4071..5099
                     /locus_tag="ACG816_000632"
     CDS             4071..5099
                     /locus_tag="ACG816_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4919545.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5105..5584
                     /locus_tag="ACG816_000633"
     CDS             5105..5584
                     /locus_tag="ACG816_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4919546.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5600..6895
                     /locus_tag="ACG816_000634"
     CDS             5600..6895
                     /locus_tag="ACG816_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4919547.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6906..7730
                     /locus_tag="ACG816_000635"
     CDS             6906..7730
                     /locus_tag="ACG816_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4919548.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7895..8794
                     /locus_tag="ACG816_000636"
     CDS             7895..8794
                     /locus_tag="ACG816_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919549.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8920..9789)
                     /locus_tag="ACG816_000637"
     CDS             complement(8920..9789)
                     /locus_tag="ACG816_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4919550.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9942..11402
                     /gene="rhaB"
                     /locus_tag="ACG816_000638"
     CDS             9942..11402
                     /gene="rhaB"
                     /locus_tag="ACG816_000638"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4919551.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11399..12655
                     /gene="rhaA"
                     /locus_tag="ACG816_000639"
     CDS             11399..12655
                     /gene="rhaA"
                     /locus_tag="ACG816_000639"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4919552.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12669..13514
                     /gene="rhaD"
                     /locus_tag="ACG816_000640"
     CDS             12669..13514
                     /gene="rhaD"
                     /locus_tag="ACG816_000640"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4919553.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13516..13827
                     /gene="rhaM"
                     /locus_tag="ACG816_000641"
     CDS             13516..13827
                     /gene="rhaM"
                     /locus_tag="ACG816_000641"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4919554.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13909..15060
                     /gene="fucO"
                     /locus_tag="ACG816_000642"
     CDS             13909..15060
                     /gene="fucO"
                     /locus_tag="ACG816_000642"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4919555.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15187..15945
                     /locus_tag="ACG816_000643"
     CDS             15187..15945
                     /locus_tag="ACG816_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4919556.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16045..16689
                     /locus_tag="ACG816_000644"
     CDS             16045..16689
                     /locus_tag="ACG816_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4919557.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16696..17703
                     /locus_tag="ACG816_000645"
     CDS             16696..17703
                     /locus_tag="ACG816_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4919558.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17719..18561
                     /gene="kduI"
                     /locus_tag="ACG816_000646"
     CDS             17719..18561
                     /gene="kduI"
                     /locus_tag="ACG816_000646"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4919559.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18581..19384
                     /locus_tag="ACG816_000647"
     CDS             18581..19384
                     /locus_tag="ACG816_000647"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4919560.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19633..20697)
                     /gene="fni"
                     /locus_tag="ACG816_000648"
     CDS             complement(19633..20697)
                     /gene="fni"
                     /locus_tag="ACG816_000648"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4919561.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20694..21779)
                     /locus_tag="ACG816_000649"
     CDS             complement(20694..21779)
                     /locus_tag="ACG816_000649"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4919562.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21792..22769)
                     /gene="mvaD"
                     /locus_tag="ACG816_000650"
     CDS             complement(21792..22769)
                     /gene="mvaD"
                     /locus_tag="ACG816_000650"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4919563.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22762..23706)
                     /gene="mvk"
                     /locus_tag="ACG816_000651"
     CDS             complement(22762..23706)
                     /gene="mvk"
                     /locus_tag="ACG816_000651"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4919564.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24022..24681
                     /locus_tag="ACG816_000652"
     CDS             24022..24681
                     /locus_tag="ACG816_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4919565.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24766..25671
                     /locus_tag="ACG816_000653"
     CDS             24766..25671
                     /locus_tag="ACG816_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4919566.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25673..26305)
                     /locus_tag="ACG816_000654"
     CDS             complement(25673..26305)
                     /locus_tag="ACG816_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4919567.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26456..26581
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26625..27149
                     /gene="infC"
                     /locus_tag="ACG816_000655"
     CDS             26625..27149
                     /gene="infC"
                     /locus_tag="ACG816_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4919568.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27221..27421
                     /gene="rpmI"
                     /locus_tag="ACG816_000656"
     CDS             27221..27421
                     /gene="rpmI"
                     /locus_tag="ACG816_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4919569.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27474..27833
                     /gene="rplT"
                     /locus_tag="ACG816_000657"
     CDS             27474..27833
                     /gene="rplT"
                     /locus_tag="ACG816_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4919570.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28085..29422
                     /locus_tag="ACG816_000658"
     CDS             28085..29422
                     /locus_tag="ACG816_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4919571.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLELV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29470..31599
                     /locus_tag="ACG816_000659"
     CDS             29470..31599
                     /locus_tag="ACG816_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS4919572.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31574..32263
                     /locus_tag="ACG816_000660"
     CDS             31574..32263
                     /locus_tag="ACG816_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919573.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32671..33284)
                     /locus_tag="ACG816_000661"
                     /pseudo
     CDS             complement(32671..33284)
                     /locus_tag="ACG816_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(33420..33614)
                     /locus_tag="ACG816_000662"
     CDS             complement(33420..33614)
                     /locus_tag="ACG816_000662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919574.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33664..34104)
                     /locus_tag="ACG816_000663"
     CDS             complement(33664..34104)
                     /locus_tag="ACG816_000663"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4919575.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34108..34953)
                     /locus_tag="ACG816_000664"
     CDS             complement(34108..34953)
                     /locus_tag="ACG816_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4919576.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35102..36520
                     /gene="pepV"
                     /locus_tag="ACG816_000665"
     CDS             35102..36520
                     /gene="pepV"
                     /locus_tag="ACG816_000665"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4919577.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36577..37524)
                     /locus_tag="ACG816_000666"
     CDS             complement(36577..37524)
                     /locus_tag="ACG816_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4919578.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37747..38097)
                     /locus_tag="ACG816_000667"
     CDS             complement(37747..38097)
                     /locus_tag="ACG816_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4919579.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38297..39376
                     /gene="pepA"
                     /locus_tag="ACG816_000668"
     CDS             38297..39376
                     /gene="pepA"
                     /locus_tag="ACG816_000668"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4919580.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39520..39840
                     /locus_tag="ACG816_000669"
     CDS             39520..39840
                     /locus_tag="ACG816_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4919581.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39862..40326
                     /locus_tag="ACG816_000670"
     CDS             39862..40326
                     /locus_tag="ACG816_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4919582.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40531..41136
                     /gene="ytpR"
                     /locus_tag="ACG816_000671"
     CDS             40531..41136
                     /gene="ytpR"
                     /locus_tag="ACG816_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4919583.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41175..42464)
                     /locus_tag="ACG816_000672"
     CDS             complement(41175..42464)
                     /locus_tag="ACG816_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4919584.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42892..43371
                     /gene="rlmH"
                     /locus_tag="ACG816_000673"
     CDS             42892..43371
                     /gene="rlmH"
                     /locus_tag="ACG816_000673"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4919585.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43456..43965
                     /locus_tag="ACG816_000674"
     CDS             43456..43965
                     /locus_tag="ACG816_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS4919586.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            44065..44715
                     /locus_tag="ACG816_000675"
     CDS             44065..44715
                     /locus_tag="ACG816_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919587.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            45166..46104
                     /locus_tag="ACG816_000676"
     CDS             45166..46104
                     /locus_tag="ACG816_000676"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4919588.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            46117..47169
                     /locus_tag="ACG816_000677"
     CDS             46117..47169
                     /locus_tag="ACG816_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4919589.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            47435..48070
                     /locus_tag="ACG816_000678"
     CDS             47435..48070
                     /locus_tag="ACG816_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4919590.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            48261..49052
                     /gene="msrA"
                     /locus_tag="ACG816_000679"
     CDS             48261..49052
                     /gene="msrA"
                     /locus_tag="ACG816_000679"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4919591.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        15687 a         8808 c        10570 g        14351 t
ORIGIN      
        1 tttatattta aaaataactg tgaaaaaaat cacgaaaaag tgtttacata gaaaaataaa
       61 tattgtatga tatacatgtg aattatttaa caaataacca tgacccatta ggaggataat
      121 gatggctaaa gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag caatgattga
      181 tgatttggca gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa
      241 agtagaccat atcgtgcatg aaatggcaat ggcagctttg gatcaacata tgccattagc
      301 gaagatggct gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat
      361 gtatgcgtcc gaatatattt ggcataatat caaacatgat aaaacagttg gtgtgatcaa
      421 tgaagatgtc caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt
      481 aactccaaca acgaatccga cttccacaac gattttcaaa tcaatgatcg cgttgaaaac
      541 aagaaatcca atcgtttttg ctttccatcc aagtgcccaa aaatcttctg cagaagcagc
      601 acgagtggta cgtgatgctg ctatcgctgc cggagctcct gaaaattgta tccaatggat
      661 cgaacatcct tcgatcgaag cgacttctat gctaatgaac catccaggta tcgcgatcgt
      721 cttagcgaca ggtggagctg gaatggtcaa gtctgcttac tctacaggaa aaccagcttt
      781 aggtgtaggt ccaggtaacg taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc
      841 agtgaacgat ttgatcgttt cgaaaacatt tgataatgga atgatttgtg cttctgaaca
      901 agctgtgatc gtggataaag aaatctatgc agcagtaaaa gcagaattcc aagcacacca
      961 agtttatatc gtaaaaccag atgaattgca aaaattagaa gatgcagtga tgaacgaagg
     1021 aaaatatgcg gtcaatccat caatcgttgg acattctgcg atggaaatcg caaaattggc
     1081 aggaatcagt gtaccaaaag gaacgaaaat gctgattgct gaattggaag gcgtaggccc
     1141 agattatccg ctatcaagag aaaaactttc tccagttctt gccatgatca aagctaacaa
     1201 cacggaccat gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat taggacatac
     1261 agcagttatc cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc
     1321 ttgtcgtatt ttagtgaata caccatctgc agaaggcggc atcggtgata tctataacga
     1381 aatgattccg tcattgactt tgggatgcgg ttcttatggt aagaactctg tttctcgtaa
     1441 cgtctctgct gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa ataacatgca
     1501 atggttcaaa ttaccgccaa aaatcttctt tgaaaagaac tctctattgt acttagagaa
     1561 aatggaaaac gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta
     1621 ctgcgataca gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt
     1681 ctcagatgtt gaaccgaacc catcgacaaa taccgtatac gcaggaacga aactgatggc
     1741 agacttcaag ccagatacag taatcgctct tggaggcggt tctgcaatgg acgcagctaa
     1801 gggaatgtgg atgttctatg aacacccaga cacatctttc tttggtgcaa aacaaaaatt
     1861 cttggatatc cgtaaacgga catataagat cgacaaacca gaaaaaacac aattcgtatg
     1921 tatcccgaca acatcaggga ctggttctga agtaacgcca tttgcggtca tcactgacag
     1981 tgaaacacat gtgaaatatc cgttagcaga ttatgcattg acgccagatg tcgcaatcgt
     2041 cgatcctcaa ttcgtcatgt cagttccagc atctgtcaca gcagataccg ggatggatgt
     2101 gttaacacat gcaatcgaat cctatgtttc agtcatggct tcagattata cacgtggatt
     2161 gagcctacaa gcaatcaagt tagtctttga ctatttagaa aaatcagtaa aaacacctga
     2221 tatggaatca cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa
     2281 cgcattccta ggtatttgtc actcagtcgc acataaaatc ggcggggaat acgggatccc
     2341 tcatggacgg acaaatgcga ttcttttacc gcatattatc cgctataatg caaaagatcc
     2401 acaaaaacat gcgatgttcc ctaaatatga ctatttccgt gcagatacag attacgcaga
     2461 tatcgcaaaa ttcttaggtc tgaaaggcga aacaacagag gaattagtcg aagctttagc
     2521 aacggctgtt tatgaattag gaaaatctgt cggcatcaac atgagtctaa aagctcaagg
     2581 agtcacacaa gaaacactag atacaaccgt agaccgtatg gctgaattag cttacgaaga
     2641 ccaatgtacg acagctaatc cgaaagaacc attgatctct gaattgaaac agatcatcat
     2701 tgatgcttac aacggataag caaaagaaag cgtagaaaaa agataagaaa atagatgaaa
     2761 aagaacttgg aaaacgttgt ttctccaagt tcttttttta atttccaagt aactgaaagt
     2821 tacttttgtt atttaacatt taggtcaatt ttagacagtt tttgttcaaa aagcgatatt
     2881 tatagtggac ggtattactg ctatattaag gatataaaat gaaagcgttt aaaaaggtgg
     2941 cgtaaagatg attattaaag aagagatttt tgtacctaaa gaattagtga aagaaaaaat
     3001 tgacctgctc gttgaaaacc tgacaacgat caaagacgat aatggtgaat ttttgttgga
     3061 ctttgatgga ctgaaagtag atgacaaaag ctggacagtg tggaattggc cacaaggtgt
     3121 cggtctatat ggtatttata aaaattacca gatgacaaaa aatcctcgcg cttatcaagt
     3181 ggtcaatgaa tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac
     3241 aatggcaccg ctattgacta tggcttactt gtatgaagac acgaaagatt cacgctattt
     3301 gccttattta gaacaatggg cagaatgggt catgaatgac atgcctcgga caaatgaaga
     3361 cgggttacag catgccacat atggaccgga aaataagaat cagttgtggg acgatacatt
     3421 gatgatgaca gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt
     3481 agaagaagct cgttaccagt ttatgatcca tatcaaatac ttgatggata aacgaacagg
     3541 attgtggtat cacggctgga cgtttgaagg aaatcataac tatgccgaag cattttgggc
     3601 tcgtggaaac tgctggttga cgattgctat tccagagatc attgaaatct tagaattatc
     3661 gaaagaagat gcattgcgca aattattgat cgaaacactt gaagcacaag tgcgtgcatt
     3721 aaaaacttat cagtctgaaa gcggtttatg gcatacgttg ttggatgatc cttcttctta
     3781 cgtagaatct tctgcaactg caggatttgc ttacggtatt ttgaaagcgg tccataaacg
     3841 ttatttacca caagaatata aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca
     3901 aatcgatgaa aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt
     3961 agatttttat cggaatatcg gaatcacagc aatgccttat ggacagtcgt taacggtgct
     4021 ttgtttgaca gaattgctcg tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa
     4081 catcactgct gtttgcagca agttcgctta gcctaggtct gctaggtcta agcggctgcg
     4141 gaaataattc tagtgtggca actgtcgcag atgcagatgt aaatgagata gttaccttgc
     4201 gctttgctta tgcaagcaac agccagccag taattgatgc aatgaatgaa tttgggcgtt
     4261 tagtaaaaga aaaaacagat ggttccgtac agatccagta tttcccagat ggacaactgg
     4321 gtggagaacg tgaactgatc gaattgacac agtcaggagc agtagatttc acaaaagtaa
     4381 gtgcctcagc gttggaaagt ttctctaaag attattcgat tttttcagtt ccttatctat
     4441 tcaatgatga agaacatttc tttaaggtga tggataatga agaaataatg gagccaatct
     4501 accaatcgac caaaaattta ggatttaccg gattgaccta ttatgattct ggtcagcgca
     4561 gcttttatat ggtagacggt ccgatccata cgccggaaga tttggaggga aagaagatcc
     4621 gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct tttaggcggt tcgcccgtac
     4681 ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa
     4741 acaatgaatt tgtcttgtat acagctggac atggtggtgt ggctaaatac tattcctatg
     4801 atgagcatac acgtgtgcca gatatcatca tcatgaatga tgccatcaaa gaacagctaa
     4861 ctgatgagca ggaaaaagcc attgaagaag cagcgaaaga atcaacggtt tttgaaattg
     4921 aagcttttgc aaaagcaatt gaagaagaaa aagctttagc acaagaagaa catggagtcc
     4981 aattcaatga tgtagataat acaccgtttc gagaggcagt tgccccactt catgatatat
     5041 tcaaaaacga tcctacctac aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc
     5101 tgtgatgaaa atgattttaa acaaggtatt agagatcctg ggagctgttt tgcttgttgg
     5161 gatggtcgct gttgttttat ggcaagtatt ttccagaacg gttttaggaa atccaaatac
     5221 ggtcacggaa gagcttgttc gattcggtct tgtctggttt gccatgttgt cttccgcgta
     5281 tgttgtggga caaaaagcac atttagccgt aacggtttta agtgaaaaat taactggcaa
     5341 aaaaagtaaa attttagaag ttgttgttca aagtttattt ctggtatttg ctgcgatgat
     5401 catggtgtat ggcggatgga atgctgtcac attgacaatg gggcagatct cgccttcttt
     5461 aagtattcct atgggatatg tttatctgtc agtacctgta tcgggggtac tcatcattat
     5521 ctatagcctg atcaatctaa tcgatacatt aagcaacaag ccagcaattc aagaaaaaat
     5581 ataaaaatgg aggaataaaa tggcaataga agccggatta gtgttatttt ttgtatttgt
     5641 tgtgttgctt gtattaggga tgccgattgc tataagtatt gcattctcat cgatgatgac
     5701 tttattgtta gttataccgt ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg
     5761 aagtatcaat agtttttcgc tgatcgctat tccgtttttc gttctctcgg gcattatcat
     5821 gaataatgga gggatagcta agaaactagt ggattttgcg atgttgttcg tcgggcgcgt
     5881 gccgggggct ttggctcata cgaatgtgtt agggaatgct ttatttggtt ccatctctag
     5941 ctcggccatt gccgcttcta ccgcgattgg cggagtgttg atccctcagc aagtggaaga
     6001 aggatatgac cggaagtttg cgacagcggt caatatcgcg tcagctccga caggtatggt
     6061 cattcctcca agtactgctt ttatcatgta ttcacttgtt gctggtggtg cttcgatctc
     6121 tgctttgttt atgggaggtt atctcgtagg cgctttgtgg gcgctaggaa tcatgctagt
     6181 agcattcatc gttgctaaaa agaataaata ccctgttgtc gaaaaaggac agatggctca
     6241 tgctggaaaa ataataaaag aggcgattcc tagccttcta ttgattgtca tcattatcgg
     6301 tggtatcttg acggggatat ttacagcagt agaagcttca gcgatagctg tcatttactc
     6361 cctgttgatt gctatgtttt actataaaac ggtaaaacta aaagatatac ctaatatgtt
     6421 gtttcaatca gtagcaatgt cagggacgat catgttctta ttggctactt cttccatgat
     6481 gtctttcgcc atggcgttta ccggtattcc tcaggcaatt agttcgttga ttatgggcgt
     6541 aacggataat aaatttatta ttctgttatt agtcaatctt gtgttattac tggtgggtat
     6601 gtttatggat gtggcgcctg ctatcttgat ttttacacca atatttttac caatcgtcac
     6661 aagtgtaggg gttgatcctg tacattacgg gttgttttca atcatgaatc tgtgtgtagg
     6721 ctccatcacg ccgccagtag gtacaggact atatgtcggt gcaagtgtag gaggcgtaaa
     6781 agcagaggca atgctgaaac cgcttcttcc tttttatggg gttatcttag tcgtcttgtt
     6841 tttgattact tacttcccgc aaatcgtcat gtggctaccc aatatgctga gttaggagaa
     6901 tagttatgtt gaacattaca attagaggac atgatctttc cgaagtccaa acgatagaag
     6961 atcttgcaga aaaaacaaaa gaacaaggga ttcatacact gcagctcgcc ttaggtcttt
     7021 cgtttcctga aacaccttct ggcgcaaatg agatcaattc tgggatggga aattatgtca
     7081 aacgagtttt agaaaagcaa gaagtgaccg taggaatttt aagttgctat atcaatatga
     7141 ttcatccaga tttaacgata cgacaagagt tgctgacaaa gttcgagcag tatcttcgct
     7201 atgcttcttc ctttggtgcg tcgatggttg cttcggaaac tggatgtgtt ttgccggaaa
     7261 tccaatatac ggaagaaaat ttcacagatg aagcatttgc tgaagctgtg tccgttatcc
     7321 gtcgcttggt aaaagctgga gaaaaatacc aaatgatggt ggggatagaa ccaggtttga
     7381 atcatccggt gtattcttta gcaagggtag agcagctgat ccaagcagta gattcggact
     7441 atctaggaat cattttagat ccgaccaatt tgatcacaag tagtgattat caagaacaag
     7501 tccaattggt agaagaagct tttgaacgtt ttggtgaaaa gatatgtgcg gttcatttga
     7561 aagattttcg tgtcgaacag gaaaaaatcg tgccggtaaa tttgggagac ggtgtgatcg
     7621 agtacacaaa aatcaaagaa atcatcatga agaatcgtcc gtacctgtat gtggttcttg
     7681 aagaaacaaa agatgatggg attcgttatg gaagaagtct tcttgaataa tgtaaagaag
     7741 acgttggtta actaatattt agaatggagg gggcggggct agaagatgcc acgtctcttc
     7801 ttttttataa gagtatataa aagcagctct ttttgattac aaaaaacgtg gtataataaa
     7861 attgtgcata agtgaacaaa aggaggtgcg gcacatgctc caaaagaaaa tcagcggtga
     7921 acaagtcaat acgatggttt ctgatcagta tgaaatctat catgtcaaag atgtcgtcag
     7981 ccaaaataaa acgatctatc atcatcatga tttctatgag atccatgcta cattaaaggg
     8041 agaagcgact ttttatttga acggacgaca atttacgatc aaagcaggaa gtgtgctgct
     8101 catccattcc aatgatttgc atcgtatcgt ccgccaatct tcagatgtat tcgagcgtgt
     8161 ctatatcttt gtcacccctt cttttctaga aagtcgttcc acgccatggt ctaatctatc
     8221 ggcttgtttt caaccaatgg gagagaagag gagtcgtgta ttgcaaatag aacaggaagt
     8281 attgaagcaa aaattatcat ttatcgacca tcctcctgat aaaaaaattt acggtgaaga
     8341 cataaaatat gaacaagctc tggtagaata cttgattttt ctcaatcaag ttgtacaaaa
     8401 agaagagagt gcgcaagaac agaatgctgt agttcctaat gagcgtgtgg aacaaatgtt
     8461 acaatatatc tcaaaaaatt tatctcatcc gctttcttta gaggaaatgg aaaagcagtt
     8521 ttttgtcagc aaataccata tcacacgaga atttaaaaag tacactggat ttacgtttca
     8581 tcagtatgta atgaagaaaa agctgatcta tgccaagcac ttgttgcgag aataccgcag
     8641 ctctagtgct gtatatagta aaagtggttt ttcttcttat ccgcattttc tgaaagcatt
     8701 caagagagag ttcggcataa cgcctaaaga atttttgaaa cgtgatacac agaaacagtt
     8761 ggttcattat gaacattttg aagatgaaca atgagaatga tcactaaaaa ataaccgcag
     8821 tagctgctgt tgtttacata gaagcagaaa ctgcggttat tttattttta tctatatatt
     8881 tcaaaatatt ttgaaaaaat gacgtacata ttatatgaat cagctgaatt tacgaaagtc
     8941 tctcggcgat agtcctgttt ttgctttgaa caggcggcta aagtaagcaa aattctcaat
     9001 ccctatttct tcggaaatca ttcgaaccgt aaattctgta taccgaagaa ggtctttagc
     9061 gtaagaaagt tttttgctga tcaaataatc gattggtgga gaaccgataa atttggaaaa
     9121 atctttgctc agctgatatt tattcaataa gaattgttgt tctaattgat caagtgtaat
     9181 cgtttctttg aaatgttcgt ctaagtattg cttcagtttg taaacgtagg cgggaatatc
     9241 ctcttcttcg aaatctagct gatacttttg caaaatcagt tcattcagca actcgtggat
     9301 agctacagag gactggaaat cggtacgggc attcgtgcct tgttgcatgt ggatcaattc
     9361 tgtgatcaaa tggtggatct tgttttctga tggatcccct gttgaatgga aggtgttatg
     9421 gccgtctttt gtgaactcct gataaaatga ttggacgttc tgaccattca aatggatcca
     9481 atccatttcc catggttctt cgctgatcgt ttgataacgc tgatattctt ggcagtcaat
     9541 aaaaaataag tctccagctt ttagctgttg ctgattatca ttatatgtaa gcgaaccttt
     9601 gccagacaaa gtaaacttta tcaagtaaga aggtaaatgt gctcgttcag tatagtaagg
     9661 gattggtgct ttgaagtaac cgatttcttg gatataaaaa aataaattcc tgcttctttg
     9721 tgtaggcgtc aagatcactc tttttgagtc ttccgaccat gagtggttgg ctttttccca
     9781 gtaatccatg taaacactcc caacttgtca agataatgca attatatacc aaaatagtgt
     9841 gaagcaaaat ataagattct gtattatgat agcattgtta ataaatgata acgctttatt
     9901 aataaagtgc taatcaacaa taaagtaaaa gaggtggaga aatgaatcca acatttgttg
     9961 ccatcgatat cggtgcatca agcggacgac taatgaaaag tacgagggat gcaaaaggtt
    10021 ctttgatctt ggaagaagta caccggttca aaaacggctt tcggaacaaa gacggtcacg
    10081 atcgctggaa gatcgattat cttatcaaag agattttgat tggatttgaa aaattaaagc
    10141 agacagggat tgatcgcctt tttgcgggga tcgatacatg ggcagtcgat tactgtctga
    10201 ttgatgaaca aggggatcgg ataaatgatc cgattgctta tcgggacaag cgtaccaatg
    10261 gagcagtaga acagttttct gaaatctatt ctctagataa gttgtatgag cagacaggga
    10321 ttcagatcca gcctttcaat accttgttcc aattgtttgc tgaagaaaaa gaaaatttgg
    10381 caaaagcaca taaattgtta ctgatacctg attatttagg gtatgtcttt actggtaaca
    10441 tggtgacgga gaaaactaat gcatctacta tgcagctgct gaatgcaggg acaaaagaat
    10501 gggaaataga acttcttgaa aaaatcggtg tctcgaagga attatttcca ccattgacag
    10561 agccgggaac gatactagga gatctgcaaa aagaacaatt ttcaaaatat gatttgccag
    10621 atgtgacctt tatcacagta gcaagtcatg atacggcgtc agctgtatta ggaacgccag
    10681 gtcaaggaga acgttggggt tatatcagca gtgggacatg gtcgctttta gggattgaaa
    10741 caaagatcgc caatgtttct cgcaatgctt tttctgaaaa ttatacgaat gaatggggtg
    10801 cccataacac ggtgcgtttt ttgaaaaaca tcatggggat gtggttgatc caggaagttg
    10861 cccgaatgca agagtacgag tacagttatg cggaattggc agagcttgct gcaaaagaaa
    10921 aaccgctgca gcaagtgatg gaaatcaatg accctcgatt tttgaatccc ccaaatatga
    10981 tagaagagat tcagcaatac tgttttgaaa cagagcagcc agtccctgtc tcatcgggag
    11041 ctatcgctcg ttgtgtgtat gataatcttg ctctttgtta cgcacaagag ctgatcaaag
    11101 tagaacactt aactggaacg aaattggaaa agctgcacat cgtagggggc ggatcgaata
    11161 atcgtctact gaatcaattg acagcagatt atgccggaat cattgtagaa gcaggaccta
    11221 gtgaagccac agcaatcggg aatgttgcca tgcaaatgct cagtacaggt cagttttcga
    11281 atatagaaca agcaagaaat gtaatcaaac agtcgtttcc atgtgaaact ttttacccag
    11341 atgaaaataa gcaaaacacg atggaatggt acgtgaataa aatcaaagga gtaatcgtat
    11401 gaacacgatt gaaagaaatt acgaacaagc aaaagaaaaa tatgcaacta tcggtgtaga
    11461 tacagatgct gtactagaaa agatacagga catcaaaatt tctatgcatt gctggcaagg
    11521 agatgatgtc aaaggtttct tgacaccgga tggcgaattg accggtggaa tcatggcgac
    11581 agggaatttt ccaggagccg cacgaacacc tgaagagctt agacaagatc tggagaaggc
    11641 ttattcgttg ataccgggca aacacaaatt gaaccttcat gctatctatt tggatacaga
    11701 agaatctgtc gatctaaatg aaattgagcc gaaacatttt gaaaaatggg tagagtgggc
    11761 aaaaaaagaa gaaatcggtc ttgacttcaa tccgactttc ttttcgcatc caatgatgaa
    11821 agacggcttt acattggctc atccagataa agaagttcgt gaatactgga tcgaacacgg
    11881 gaaaagaagc cgcaaaatcg cggagtactt tgggaaagaa actgggcaaa caagtatcaa
    11941 taacttttgg gtacctgatg ggatgaaaga taatccgatt gatcgtttta ccccacggga
    12001 acgtttaatg gaatcactcg atgaaatttt tacagaagag ttagatgaac ggtatgtaca
    12061 agatgcagta gaaagcaaac tatttggttt aggcgctgaa gcttatacag tcggttccca
    12121 tgaattttat atgggttacg ggttgactcg tgacaaactt gtttgtctag atgctggaca
    12181 ttttcatcca acagaagtga tttccaataa attatcttca ttagctttat tcagcaaagg
    12241 aatcatgttg catgtttccc gtccagtgcg ttgggattca gaccatgtgg ttttgatgga
    12301 tgatgaattg caggaaatcg ctaaagaatt agtccgtaac gaattgttgg aaaaaacaaa
    12361 tatcggtctg gatttctttg atgcaacgat caaccgtatc gctgcttggg tcatcggtac
    12421 gagaaataca caaaaagcac tgctgaaagc tatgctggaa ccagttgagc gattgaaaga
    12481 aatggagctg gcttttgact ttacttcaag aatggcttat acggaagaat tgaaagattt
    12541 cccgtatgcc gatgtatgga attatttctg ttacaaaaat caagttccag ttggtttgga
    12601 ttggctggaa gaagtccagg aatatcaaaa agacgtgttg gaattaagaa aataggagtg
    12661 tggctatatt gaaaaaaata gatattttac aagcgcctta tgtgaaagaa atgatcgaaa
    12721 cgacgtcgaa tctttatcgc ttgggatggg atgaaagaaa tggcgggaat atttcttatt
    12781 tactaaaaga agaagagatc acaccgttct tggatcctga aaaagtgatc cgccgcatct
    12841 cgatgatttt tgatgccagc cgtttagcag gatgctattt tattgtaact ggttcaggaa
    12901 gatattttaa aaatgtagcg agcgaacctg ccgagaatct tggaatcgtt cgagttgctg
    12961 aagatggcaa aacactggaa cttttgtggg ggctggaaaa cgaatgcctt cctacaagcg
    13021 agttgccaag tcatttcatg agtcatatcg cccgtttaga agtagaccca gataatcgga
    13081 tcgttatgca ttgccatgct agtcatctat tagcgatgag ttttacgcat gaattagacg
    13141 aacgttcctt tagtcgtacg ctatggcaaa tgtgtacgga atgcttagtt gtttttccag
    13201 aaggaatttc catcattccg tggatggttc cgggaacaaa tgagattggg gaagcaacag
    13261 ctgaaaaaat gaaagaaact cgattggttt tatggccaca acatggaatc tacgggtcag
    13321 gaaaagacat ggatgaagtc tttggtttga tcgagacagc agaaaaggca gcagaagtct
    13381 atacgtacgt caaagcgcaa ggaccaatct tgcaaacaat cacggacgaa aacctgtggc
    13441 atttagcaga tgcgtttggt gtcactccaa aagcaggtta tttggaggaa gttcatacga
    13501 aagcaggtgt ctgacatggt gaaaaaagct ttttgcatga aagtttaccc agaaaaacaa
    13561 gaagaataca aaaaaagaca tgatgaactt tggccagaaa tgcgtacagc acttcgtgaa
    13621 cacggggtca tcgactattc gatctatctt gatccgagta caagtttgtt atttggttac
    13681 ttagtgatcg aagatgaaaa gttatgggaa aaaatggcaa atacatctat caatcaaaaa
    13741 tggtggaagt acatggaaga tatcatggag acaaatccag atgcctctcc agtcagttgg
    13801 gaattgatgc cagtctttga actgtagcaa caagcaactg ggcaaccggt tgcttggggt
    13861 tctttcgttt ttattgtgaa aaaaaataca aattaggagg aatagaaaat gagtaatcga
    13921 atgattttaa atgaaacatc gtattttgga aaaggagcga ttgagtcgat cgtccctgaa
    13981 tttcagcagc gaggattcgg caaagcagct gtcattactg acaaaggatt gatcgagcac
    14041 ggtatagcaa caaaagtaac agaacttcta gataaagcag caatcgctta tgctttatac
    14101 gacgggatcg tacctaatcc aacgatccaa aatgtaaaag atggagtggc ttttgttaaa
    14161 gaggcagagg cagactgttt gatcgcaatc ggtggaggtt cgccaatcga tacggcaaaa
    14221 gccattggga ttattttgac gaatccagag ttttcagacg tcattagttt agaaggtgtt
    14281 gctgatacga aaaatccatg tttacctata ttagctatac caacgacttc tggaacagca
    14341 gcagaagtaa cgatcaacta cgtcatcaca gatgaagtaa atcaccgaaa atttgtttgt
    14401 gttgatcctc atgatatccc aatcgtcgcg ttcatcgaca gcgatatgat gatgggaatg
    14461 ccgaaaaaat tggctgcatc aacaggtatg gatgctatga cgcatgctat tgaagggttt
    14521 atcacaaaag gcgcttggga aatgactgat atgctgcatt taaaagcaat cgaaatcatc
    14581 ggccattctt tggaagtttc tgtagatggc gatcaaaatg ggcgagaaaa aatggcatta
    14641 ggacagtata ttgcgggaat gggcttctca aatgtcggtt taggactggt acacgggatg
    14701 gctcacccgt taagtgcatg gtacaacatt cctcatggcg tagcgtgtgc agcacttttg
    14761 ccaactgtca tgaaatataa caaagaatat actggagaaa agtaccgtga gatcgctctt
    14821 gttctaggga ttaaaggagc agctgaaatg tctttagaag atgtaagaga agcggcttgt
    14881 ggtgagatcg atcggttgag caaagcggtt ggaatcccag aaaccatttc tgaattaggt
    14941 gtaaaagaag cagatattcc agcaattgca gaagatgctt tgcgagatgt atgcacacca
    15001 ggaaatccaa gagaaacgac agtagaagaa atcattgctt tatatcaaag tttgatgtaa
    15061 aaacgaagcg gatcttcaac attaaattgc ctgtaaagag aaatggtagt ttatatctgg
    15121 atatctcttt taaattgatg gtatttccgc tataattttt cttataaaga aataggagaa
    15181 tattatatgg aaaataaaaa gccttatgga actgtattga tcaaagcctc taaaatacta
    15241 gactatttag cggaaaacca agatgtctct ttgcagacga tcgcaaaggg tgtgggaatg
    15301 acctcttcta cagtcttgaa aatactagat actttgttga tgatcggcta cgtaaataaa
    15361 aattcagaga aaaactatcg tttaggtgca aagcttattc gctacgcgaa taaaaacatt
    15421 gaacaaatcg atttggtaga gttgacttta ccgtttttag aacagctgca gcaaaagatc
    15481 gatgagacta tccacttagg ggtattggac aacaatgaga tcttgtacgt gaacaaacta
    15541 gatccaaaac atcaaacgat ccgcatgtct tcaaaaatcg gaatcacccg accattatac
    15601 aattctgcga tgggcaaagc cgtattagca gaattcagtg aagagcagta cactgattat
    15661 cttgaaaagc atccgttgat cccttacaca gaatatacga tcaccaatgc attacggttg
    15721 aaaaaggaaa tccaaacggt aaaagaaacc cacatcgcat ttgacgatga agaaatcgaa
    15781 cgtgatattt tttgtatagg agcctcaatc gtgaaaaacg aacaaatcat tggtgcattt
    15841 agtgtaagta tgccgaaata tcgtttaaca gatgagacaa aagaacaaat aatcagtgcc
    15901 ctcaagcaaa caaaaagcga aatcgagcaa agaattcaaa aataatgaaa aaagcgtttg
    15961 caatttttct tttctgccgt ataatatggg tatagatttc caagttataa aataaaattt
    16021 ctaaatagga aataggtgga gaagatgaaa aaaattgata ttttatctcg attgaaaaac
    16081 gccggagtga ttgcggtggt acgcggaaaa agcaaagaag aagctttgaa tgcttgccac
    16141 gcaatcatca aaggtggtct aactggtatt gagttgactt ttacagtccc tcaagcagat
    16201 caagtaatca aagaactttt gtcattctac aaagaccaac cagaaatagt gattggcgca
    16261 ggcacagtat tagacgctgt cacagctcgc ttggctatct tagctggcgc agagtatatt
    16321 gttagccctt cctttgatca agaaacggca gagatgtgca atctgtatca aattccttac
    16381 ttaccaggat gtatgactat taccgagatc aaaacagcgc tgaaaagcgg ggtagatatc
    16441 gtcaaactat ttcctggaag cgcttatgga ccaagtatca tttctgcctt caaggcgccg
    16501 atgcctcaag tcaatatcat gccaactggc ggtgtgagtc tggacaatat gaaagagtgg
    16561 ttcgatgctg gtgtcgtaac agtcggagta ggtgggaacc ttttagcacc cgcagctaca
    16621 ggagattttg acaaagtaac ggaagtcgcg caacagtatg cagcaaaaat gaaagaaatc
    16681 aagaggtagc tgcttatggg aaaagtagtg acactcggag aaatcatgtt gcgattgtcc
    16741 acagagcagg gaacccgtat cgattcaagt gaacgattcg ctgcccatta tggcggaggg
    16801 gaagcaaacg tagcaatttc tttagctaat tacggacatg aagcgatttt tgctacaaaa
    16861 gtacctgata acgcattggg aaaagcagtc aaaaagcact tgcaatgtta tggagtagat
    16921 acgaaacagg tattatctgg tggatcaaga ctaggtactt attatatgga aactggtagt
    16981 ggcgaacgcg ccgcctcagt gatctatgat cgtgccggat caagttttgc tgaaatggat
    17041 catttggaat ggtcgctaga ggagttattc caaggaatag atatatttca tgtttcagga
    17101 atcaccccag ctttgtctaa aaaatggcaa aaaatgacga aacaggtgat agaagctgca
    17161 aaaaaagctg gctgcctgat cagcttcgat atcaactatc gtggaaaatt atggacacag
    17221 caagaagcca gcgaagtaat ccatcagctt ttacctttag tagatatctg ctcagctgga
    17281 aaaatggatg ctctttattt gttaggtatc gagaaagcgc cagaagcaga agaacaacct
    17341 ctaatttatt attatcaaaa aatgcaggaa aagttcccga atattcaggt gttttattcg
    17401 accaagcgta ccgttcattc agcaagtgaa aatgaattga aggggacatt gtggatgaac
    17461 catgaatatg tggaatccca agtccataga atcaccccaa tcgtggatcg agtaggtggc
    17521 ggagatgctt ttgcaggtgg cgtgctccat gggattttat ccaatatgtc tccgcaagaa
    17581 atcattgatt ttgctacggc agcttctgcc ttgaagcata cgatccatgg ggactgcaac
    17641 caatttagtc aagaagaagt cgcacaattt ttagcatgcg gctctggaaa aatcattcga
    17701 tagaaggagt agaagaaaat gcaagaaatg gaaacacgtt atacacacag tccagaagat
    17761 atccgtcatt attcaacgga acaattgaga aaagaatttt tagtagaaaa ggtatttgaa
    17821 ccagggaaaa tcagtttgac ttatacgcac aacgatcgaa tgatctttgg tggagtgaca
    17881 cctactgaaa aagaattaga aatcacatta gacaaagaac taggtgttga ttacttctta
    17941 gagcgtcgtg aactaggtgt gatcaatatc ggtggaccag gattcatcga gatcgatggg
    18001 caaaaagaag aaatgaaaaa acaagacgga tactatatcg gaaaagaaac acgacatgtt
    18061 gtattttctt ctcaagatgc tgaaaaccca gctaaattct atattagttc tgttccagct
    18121 catcataaat atccaaatgt aaaaatcagt atcgaccaag taaaaccaat ggagactggt
    18181 gaaggcctga cactgaatga acgtaaaatc taccaataca tccatccaaa tgtatgcgaa
    18241 agctgtcaat tacaaatggg ttatacgatt ttagaaccag gaagcgcttg gaatacgatg
    18301 ccttgtcata cacatgaacg tcgtatggaa gcttatgtct actttgatta tgccaatgaa
    18361 gatacacgtg tgttccacat gatgggcaaa ccagatgaaa caaaacattt agttgtagac
    18421 aatgaacaag ccattatttc gccaagctgg tcgatccatt caggtgtagg tacaagcaat
    18481 tactcattca tctgggcaat gtgcggagaa aatatcacct atacagacat ggatatggtg
    18541 caaatggatc aattaaaata agcataaggg agagaaaatg atggaattta gtatggatat
    18601 gtttcgttta gatggaaaag tagcattagt caccggtgct gtctatggta ttggatttga
    18661 gattgcacgc tctttagcta cagcaggagc aaaaatcgta tttaataact tgaatcaaga
    18721 atcagttgat gaaggtatag cgcattataa agaagcaggc atcgatgcaa aaggttatgt
    18781 ctgcgatgtg accgatgaag aagcggttca agcgatggtt caacagatca aagaagatgt
    18841 aggttcagtt gatattctag tcaacaatgc agggatcatc aaacggacac cgatgatcga
    18901 aatggatgcg gcagacttcc gccaagtaat cgacgtggat ttaaatgccc cattcatcgt
    18961 ttcaaaagca gttattccgg atatgatcga aaaaggcggc ggtaaaatca tcaatatctg
    19021 ctccatgatg agtgaattag gacgcgaaac ggtcagtgct tatgctgcgg caaaaggcgg
    19081 attaaaaatg ctgacgaaaa acatcgcttc tgaatacggt caatacaata tccaatgtaa
    19141 tggaattgga ccaggttata ttgctacacc gcaaactgct ccattacgcg aaacacaaga
    19201 aaacggtgaa cgtcatccat tcgatcaatt tatcgttgga cgtacgccag cagcacgctg
    19261 gggcgagcca atcgatttgg ctggaccatc tgtcttcttg gcttctagcg catctgattt
    19321 cgttaacggc catgtcttgt atgtggacgg aggcatactt gcctatatcg gaaaacaacc
    19381 ttaaaagagc tttagtacac tgtttagtgg agaggttgtg aaatcagcgg attgaacgaa
    19441 taccgaagga cagctgatta aacatcattt attcgaaaaa agaggctggg acagaagcat
    19501 ttaactccaa gaaataagaa ggaattcacg aaaattgctt ttcaaatttt tgtgaatttc
    19561 agcttatttc cgaaggagtt gcttttactt ccgccgtttg tacgtgttta gagcgtgaga
    19621 caaaagtgtt ctttactttt gtcccacgct cttttctgtg cgtcttccat acttcactaa
    19681 gtcgatccct cttgcttcgc accaatcctt cactggtccg gaaaagatca gagattgttg
    19741 gtgtagggcc gcggtattcg ttgcgccgac catagtataa agcagacgaa gctcttcttg
    19801 ccattgcttc atcagtataa tagtttcttc tacgccgtga ttcatcagat gcgtcagtac
    19861 ggttcctgaa gtacccacgg catttgcgcc gagacaaagg gctttaaata tgtcataggc
    19921 attgcggata cctccggagg ctaggatttc catagaagtg tctgcttcat ttgcttcaag
    19981 caatgaagag actgtcgatt gcccccagtc agctaaataa ctcagttccc gcttggagcg
    20041 acgggcgttt tcgatctggg tgaagcttgt tccgcttcga ccgctgatat caactgtatg
    20101 aactcctaaa gcagctagat cattgagcgt ttctcttgtc atgccaaaac cgacttcttt
    20161 tacgatgagg gggacgtcaa tggctgtttg agtttcttga ataagtactt tccaattggt
    20221 aaaatcacgg tctccctcag gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa
    20281 ggcatctgcg tgaaacaatc ggatagcttc tttggctctt tcgacagaag tccctgcacc
    20341 gatgttggca atgattttcc cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc
    20401 taaagaaggg actttcaagg cagcgctgac tgaaccagtg gcaatcatca aatctgcttc
    20461 tcttgcgacg attgcaagat cttggttgat tttttttgtt ttttcgcttc cgccagtcat
    20521 cgcattgata taaaatgggc tgcttagtgt aaacccaact gcctgtgtac ttagatcgac
    20581 atcagaaact gctatttgcg gcaaagggtt atggatgatc cgtacgaagt caaattcgtt
    20641 tttttgttta tcatgaaagg ctttagctag tgaaacatgt tcgtcttttc gattcatcgg
    20701 ttttcctttc tttgatcgct atagacatgt aacggcagtg gagtgatttc tgctttttcc
    20761 catgcactca ttaaaggaag aatgccagat ttctggtcaa caattacgat tccgcaatcg
    20821 cccccacctg caccagaaga ttttgcggct ccttcatact gttcagctaa attacacaat
    20881 ttgttcaaag caggcgtttc gatgacgacc ccagtgattg cagataaatc atgcagtaat
    20941 tgtcggtttt ttcgaatcat ctgttggatc aacgttacat tattttcttt aaaccctttg
    21001 atcatttcgt tgacacattc tgtactattt tttaagaaaa gctgataagc caccatttta
    21061 tcttctctcg aacggtgaac ttgatcgacc aaatcagaag tagaggcagg gctacccgtc
    21121 caaccaatca ataaacgtaa atcttcagga gcaatcaatg gctcaatgga tagacctggc
    21181 caatccaggg caagtaactc actgatagaa tgctgagttt cttgttcttg gagccaagga
    21241 tggtcgaagg ttgagaaagc gatccagcca ccataacagc tagcagcgat gtcgccgcag
    21301 gaaccattat cttgaactgc taaattggct agtgctgcaa tcttgaaaat ctccaactga
    21361 gataaattca acgcgtaaaa aacattcaag gctttgaccg ttgcaacggt cacggcaccg
    21421 ctagaaccca atccatattt ccggccattt gaactgtcta actcacttgt tactttcaga
    21481 tcataaaagg ataaaagaat gtttttttct tgtgcatact tttcagtcaa gcgaatagca
    21541 gcaaggatat aatgaaaagg attttcccga atatctaaaa ctaattctcc gttgcgtctt
    21601 gtccaacgta caggcatccc actatattgt gcagattgga tacttccgac ttttcgtgca
    21661 gattctacag tgactgtcac gaattgatcg actgcagcga taactgctgg atggcctgtt
    21721 tcaacaactg catattctcc ggcaatatag agtttgcctg gtgcagatac ttcaatcata
    21781 aaaatctccc tttattcaat aatcgcaatt cctggaccag catgtgcagt aattacttgt
    21841 tctttgctaa ataatttggt aaatgtttct tgtacctcag ataaatggct gttttccact
    21901 aaaactttga cattagggcc agcatccatc gtaaagtagc atggaatgcc ttgctttctc
    21961 aattgtcgca ccgcatccat cgcttttaag ctgtcaggag accagtaagt gaatggaggc
    22021 tgggcagcta aagtcgttcc atgcattttt agtccgtttc tttccatcgt ttctccgaga
    22081 agttggaaat cttttgtttt gattgcttgt ttcaattgat atagatcccc ttcaacggaa
    22141 tctaaccagc cttgataaaa actggatgtt tcgactgtcc gacgcatccc atttctgctg
    22201 gacacttctt ttttctggtc gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa
    22261 gaatcggaag ggactggctt agcgtaagaa cttaagtcgt catggccttt ttcccattcg
    22321 acgaaaccac cgaaaatact tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa
    22381 agagagagat catctaatcc aagcttaaga gcagtattgc aagcgcctgc taaagcagct
    22441 agcccgctgg cagaggaggc taatccagct gcggtaggaa cgaaattctg actgattact
    22501 gaagctttta gtgagaggcc ggcttcttta cgaaaaaggt caagaaactg actgactttt
    22561 tttgttgctt tttcgtcttg caaggtgcca tctagataaa attcatctac catatagcta
    22621 tcagaaaaga tgacttctgt ctctgtataa aatgcatcca atgttaacga aaggctgttg
    22681 ttcattggaa ggatgagttc ttcatttttc ttaccccaat atttgattag agcaatgttg
    22741 gtatatgcgc gtgctttgcc tttaaacata ggtatgtact cctaatcctt gtatccaagt
    22801 ttcagcagca cccgcatctt caagtgcttg actgatttct tgggcttttg tttttgtttg
    22861 tgctaatgca atcatacagc caccgcgtcc accgccagtt aatttggcac ccaatgcccc
    22921 ggtatctttt gctgtttgga tcaatagatt aagaaaatca ttgctgattg tcagcttttc
    22981 cagaagtgat tgtgattcat ccattgtctg tgccaacgtt tctggtgaat tttcaatgat
    23041 tgcttgtttt gcttgcttcg tcaagtatcc taatttttgg atcatttgtc cagtttcatg
    23101 aggctgggtt tcaaagaggt gagcgacatc tttgacggct tctcttgttt gtcctttgat
    23161 tcctgtatca gcgacaatca aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg
    23221 cccttttgta aaataaatcg gctgcaagct gctagtagcg gctgcatcga ttccactagg
    23281 attaccgtgg gcgatttttt ccgaaagctg gacattttct aatagtattt cacgagacaa
    23341 aggaaatgct aagtaatcat aaaaagcacg agtgactgct gtggcgactg cagcgcttga
    23401 tcccattcca cgttcagcag gaatcgtgct ttcaatggtc agttgcaagt ttttttggat
    23461 gtcatgctgt ttttttaagt gaatgaataa ttttttgata ttttttaatg catgaggagc
    23521 ttcggctaaa tttcctgaat agtaggaaga aaccagttga tcgtccattg catccagctc
    23581 ttcaaggaat gcggtgactt ttgttgcata gaaaggaaag gcaatcgctg gttctccata
    23641 aacaaccgcg tgctcgccca tcaatatgat ctttccgctt gattctcctt ggccatagtt
    23701 tgccataatc taactccttc tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca
    23761 aaacgagttg cgctgcttca cgaatttttt tgtccactaa gggtctcaaa aaacattcct
    23821 cctttatttt actaaaacca tctcagaagt aaaactaaga atacgttttc taaagaaaaa
    23881 acactttttt cttaagaatt ttttgacttt ttttcttctt tgactagaaa aaaatacaat
    23941 tgacactact agatattggg ggtatattta aaaaggttgg agttaaacac aatatttagt
    24001 gatgaagtga gctggaggta aatgagcgtg atcgttttag caggtacaat tggtgccgga
    24061 aaatcaagtt taacagaaat gatggctgaa cactttgata gtcaggcatt ttatgaatcg
    24121 attgatgata atgaagtctt gcccttattt tatgcaaacc ctgaacagta tgcttttttg
    24181 ctgcaaattt attttttgaa taaacgtttt gcaagcatca aacaagcaat gaaagatgac
    24241 aataatgtat tggaccgttc gatttatgaa gattcactgc ttttccatct aaatgcagat
    24301 ctaggaagag caacagaaac agaagttcgt gtgtatgatg aactgctgga aaacatgatg
    24361 gaagaacttc cctatgctgc ccataaaaaa catccggatt tattagttca tattcgtgta
    24421 tcttttgata cgatgttgga gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc
    24481 tttgatccaa gtctatacga ttattacaaa gaattgaaca gacgttacga tcaatggtat
    24541 gaagaataca aagaaagtcc aaaaatccaa atcgatggag accgctataa ttttgtggaa
    24601 gatcctcaag caaaagaaga agtcttgaaa atgatcgaag aaaaattagc agagattcgc
    24661 caaaccaaag tagccatttg atttacagtt gctcaaataa agggtaaacc tttatttgag
    24721 cgactttttt ctttatactg gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac
    24781 tattttaatc aagaccgtaa tttttggatc aatgtacagc aaaacgatgt gctagctgtt
    24841 aaaaacttaa aagaagaata tggcattagt gacgagatgc tgacttattc tctagataaa
    24901 aatgaacggg cgcgcgtaga gtacgattct tttgatgaag cgttactgct tgtatcaaat
    24961 gttccgcacc agcaaaaagt cgataatcat tatgaaacta gtcctatcgc ttttatttta
    25021 aaagaagacg gtttgtttac atttaccacg ccgaataccg agtacgtgat tcgactaatt
    25081 cgatcgcttt tggaacgaat gccagacatg agtgtataca gtttattgtt tcgtacattg
    25141 tttttgattt ctgattcctt ttttccttta atcgaggaag tgaatagtga acgtcagcgg
    25201 cttaatctga aattaagaga aaaaacaacg aacaagaatc tgctgcaact ttcagattta
    25261 gagatcggtt tggtttactt agttacagga acaaaacaaa atgtagtact tttagaacaa
    25321 atcaaggcat tggctattta tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat
    25381 gcgctgatcg aagcacgaca agcagttgaa atgacgaact tagcttcgca gattttagac
    25441 cagctctctg gcacttataa taatttgctg aataacaact tgaatgacac gatgaaattt
    25501 ttgacggtct ggtcgctgat cttgaccgtt ccaactattg tgacaggatt cttcggaatg
    25561 aatctccagt taccctttac ccattccgtt ttcggatggg gaatcgcctt gatcatcagt
    25621 ttggtcttat ctatttggat gcttattgca ctgtggcggc ggattcgttg atctagtctt
    25681 tgatctgcaa accaagggct tgggcgatca caagtgcttg ctcttggttg acgattagtc
    25741 ccgccaaaga atcttgagat aaagcgattg ctgtgaagaa attagtcgta aagtctaatc
    25801 ccttcagaga agttcgaaac cagttcgaac cggtcaactg attgtcagac aaaaaaaggt
    25861 ttttccaagt gacctcataa aattcgctgt cttctaattg acaatgatcg aaggagacag
    25921 ctttcaagtt tgtactacta aaggaagcca tcgaagcaat acaatcagtg aaagtacagt
    25981 ctcgaagata actttcggca aaattcgtac ctgttaattt acattgatga aacacgactt
    26041 ggtggaaact gccaccaatc cactccagat tggaaaaatc acatttttca aaaatgacat
    26101 tactgcattc aaaatgacgt aaaactgttt tttgcatcgt aagtttttgg atatgactct
    26161 ctcttaatac catatttttg gcatcgagat agctttggtc acccccgttg atcagaagac
    26221 cagagaagat ctcctcatct tctaaataaa actctccctt tgtaagaaga ggaagagaag
    26281 gtggaatagg ttcgattttt ttcatgtggg ttcacctcgt ttttctttag tatagcaaag
    26341 aagcgggaat cactgctaga aagtctgcta atctaatcat tttcagaaaa ggaagacaaa
    26401 ctgtatgaaa aacattgaca aaaagattag tttttgctat actatgatgg ttgagaaata
    26461 aaagcagaag cacccgcttc tcgccttagt tgataatgtc actgggctag atagttttca
    26521 ctggttctat taggtgaagt acgcgcgggt tctgtaagag aactcgtttt tttattgccg
    26581 tttattcggt aagcttttct ttctctcata atatttggag gtgaatgacc atagcaaagg
    26641 atatgatggt taacgacggc attcgtgcac gagagttacg attgatcggt tcagatggtg
    26701 agcaattagg agttaagaca aaagcagaag cattgaatat tgcagaacaa gcaaacttgg
    26761 atcttgtgtt agttgcccct ggtgcgaaac caccagttgc gcgaatcatg gactacggta
    26821 aataccgttt cgaacaacaa aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca
    26881 atgtaaaaga agttcgctta agtcctacga tcgacttgaa tgacttcaat acaaaacttc
    26941 gtaatgcacg taagttcttg gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag
    27001 gccgtgccat tacccataaa gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa
    27061 ctgcggatat tgcaacagtg gaacaaaaag cgaaaatgga cggacgcagc atgttcttga
    27121 cgctggcacc gaaaaacgac agtaagtaat ttgaatgact aactggctgt tggacagccg
    27181 atagtcggat aacgaaagat tttgaggagg aaatatagtc atgccaaaac aaaaaacaca
    27241 ccgcggatca gcaaaacgtt tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc
    27301 gtttacaagt caccgtttcc acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc
    27361 aagcatggtt tctagtggcg acttcaaacg tattcgccaa caactagcaa aaatgaaata
    27421 agaagtacaa ataacagaat ctgaagattt atttttatta ggaggaatta tacatggcac
    27481 gtgttaaagg tggaacagta actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag
    27541 gttactacgg ttcaaaacac actttattta aaacagcaaa agaacaagta atgaattcat
    27601 acaactacgc atatcgcgat cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg
    27661 ctcgtatcaa cgcagcagct cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt
    27721 tgaaagttgc tgaaatcgac atcaaccgta aaatgttagc tgacttggct gttaacgatg
    27781 cagcagcatt tactgcattg gctgaccaag caaaagaagc tttggctaaa taagctaaac
    27841 aattataaag aggaaagaag ataacgaaaa gttatgttct ttcctctttt tttatttaca
    27901 atcctccatc aaatgaaaca ggacaataac gtataagtct aaaatagcat aattaaggtt
    27961 ttttaattga aaactgaaca tttatggtca ccctttaatg aaagcgctaa ctacataagt
    28021 tagaatgagt ttgactgata tattagtttg cttatcagaa aaacaaaatg gacggaggaa
    28081 taacatgagt gatattatta ttggtatttt gttagttatc tcattctttt tcatggtctg
    28141 gtactgtgta aaaggctaca acttgatggt cggctttgcc atcatggcta ctgtatggat
    28201 gggattagag ctagtcggaa acacgttttc accaaacccg gcgatggaag ggcaaggagt
    28261 aattgatgtg ttgacgcata tctatacgac ggggccagct gagtacgcca aatcgatctt
    28321 ggtaaatgtc ttctttggtg cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc
    28381 taccctgatt cgaaaggtag ttgaacttgg aggggacaag ccaagaatca cgatgagctt
    28441 actatgtatt gtaaccgctg ttatattcat gtcgatgaca ggaatcggac cagtgatttc
    28501 tattgcagtt attgtgttgc caatcttgat gtcattaggt atttcagtac cggttgcact
    28561 gttctcattt atgggctcga ttatggctgg aatctttgct aatatcgtca atttcaaaca
    28621 atatcaaacg atttatgctg gtttcaatcc agcggctgaa agctatactt ataatgacta
    28681 tttccaaatt ggtatgattg ggatgattgt aagcttagtt gtggtactaa cggtcgctaa
    28741 tatatcaatg aacaaaaaga aacgctatgc aatggcagca aacgtaccag ctgaaggtgg
    28801 cgacgcacca atgatctcat ggttagccgt attattacca gttttagggg tcgttctttt
    28861 agatcttcca atcatcttag gttttattct agcaggtatc tgggcattgc tgtttactgg
    28921 aaaattgcgc ggaggttata aagaaatctg tcgtcagttt gcgaaattat ttacagatgg
    28981 cgcagtagat gttgcaccaa tggttggttt cttgatgacc ttagcgatgt tcaataattc
    29041 agctgcgtat gcttctcctt atttttcagc tatttttgga gattggatac cgcaatcacc
    29101 attagtcttg gcaatcgtat ttgctatatt gacaccacta ggatttttcc gcggtccaat
    29161 gaatctagtc ggttcaggct cagctatttt agctgtcgta ttagctgtta atccaacgat
    29221 gtcgccagcc tttttgttcc cgttatttgc aatcacaacg attgctcctc agcatttaga
    29281 tattacacaa tcttgggttg cttggggatt aggttataca aaagtaactt ctcgagaata
    29341 tatgaagaaa tccatcccaa ctggttggat catcggagcg atcttatgcc tgatcacgtt
    29401 cttattgtac gggaacgcct agtaaacaaa acgtcgataa aaacgattag atgaatggag
    29461 gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa
    29521 gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc
    29581 cacgatgatg tccgtggagt agccgcaggt gtcgttcagt cttttcaaaa ttgtttgcgg
    29641 gaaaataata tcagtccaga agatgttgtg tttgttgcac attctaccac acaagcgact
    29701 aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga
    29761 ttagaaggtt ttttagcaaa acgacaaact agattgaacg atatcgattt agggaataag
    29821 aaaaaaatag agattgtcaa tgcattctta cctgtcaaac acttaaatgt agaccgtgtt
    29881 tcagaaacga tttcttcttt agaaagagaa agagcggagg tcttggtttc ttctatggca
    29941 tttggagtag ataatgggga accagaacga gttgtttatg aagcagcatc ggtaaaatcg
    30001 atccctacaa cgatggcttc tgacatcaca aaattatatg gattgacaag aagaactcgg
    30061 acagcagcaa tcaatgcatc tattttacca aaaatgctag atacggcaac ctctacagaa
    30121 gattccgtac gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga
    30181 gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc cggtccagca
    30241 gcatcagtca tgggctcttt gatgtacttg agagcgtcta atggtgttta ttttgaagta
    30301 ggcggaacaa caaccaatat cggggtcatt aaaaatggac gtccagcaat cgattattca
    30361 attgtaggtg gccacccaac atatatctct tcattagatg ttcgggttct aggtgtggca
    30421 ggcggttcga tggtacgggc taatcagtca ggaatcatcg atgtcggtcc acgttctgca
    30481 catattgccg gtctagatta tgcagtattt acggagacag aaaaaatcaa aggtcctaag
    30541 gttgaatttt tctctcctaa agaaggagac cctgctgatt acgtcaaggt agtgatggaa
    30601 gacggagaag aagtgactat tacaaataca tgtgcagcca atgtccttgg attagtgcaa
    30661 gaagaacatt tttcttatgg aaatgtccca tctgcacgaa aagccatcca agcattagct
    30721 gattattgtc atacgacggt tgaagacatc gcagaacaga tcatggaaaa atcctatgct
    30781 aagattgaac cggtgatttt agaattggca gacaagtatc acttggagaa agatcagatt
    30841 tctcttgtag gtgtaggggg cggcgctgcc tcattgatta cttactttag taataaaatg
    30901 ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt cttctattgg tgtagcactt
    30961 gcaatggtcc gagatgttgt agaacgtatc atcccttcgc caagtaaaga agatatacga
    31021 tcgttgaaaa atgaagcgat gaataaagcg attgaatcag gcgctacacc agaatcgatc
    31081 gaagtccacg tagaaatcga tccacagact tcaaaagtaa cggctatcgc tactggttct
    31141 acggaagtga aagcaaccga tctaaccaag gagatcacaa cagaagaagc actagagctt
    31201 gctgcagagg atatgcgatt aaataaaaat gaagtttgct tattggaaaa tacacctttc
    31261 ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat
    31321 caaaaaggat ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac aactgcagcc
    31381 aattatatga cagctgtcga acaactttgg gaagatatgg cagtttatca aactgaattg
    31441 attgcccgac cagaatttta tctctgctta ggagcaagag tctctgattt tacagcgacg
    31501 gatctggaac aactacagct tttgatggat ctggaagtat ctactatgga accggaggaa
    31561 gaagtgatcg tcgttgccgg aaatatcaaa caaacataag ttagaagagt tactaaacga
    31621 attacaacag atccccgaag aaatttgggg attctaccag tttcggcgag atttattttg
    31681 gaaaaagatt cctttgtcca agcagaaaat tcttatacaa caatcaatcg attgtggtat
    31741 cgaaacggca tgtagcatca aaaaaaagta cccttttgcc gatgtaggag aaatttgtga
    31801 acaaatggct gttccaatcg tttcctgtga atcagagcaa ataaacgaac gaatcacttt
    31861 tgcaacttat gcagaagatg aagggattcg attgatgacg gagccgttag aaaaattaaa
    31921 atgttcagga ctagcttcga tctctaaaga aacagcgcaa gctttgatta ttggacatga
    31981 actatttcac cacatcgaag cttctgttaa gggaatttat acgcaaagcg aaaaaattgt
    32041 gctgtggcgg ttgccttttt atacacatca atcaaacatt cgtgcattga gcgagatagc
    32101 agcgatgagt ttttcaaaag agatgaatca aagcgatttt tcgccttatg tattagaggc
    32161 agtcttactt tggccatata atgaaattcg tagccaaggg atcttagaag aaataaaaaa
    32221 aatagaaaaa agatgtacgg agtatgattt agattacaaa taaagagact gtgacaaaag
    32281 tcgtgtcaca gtcctttttt tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca
    32341 agtcaaaata ttcaacaaac atgagaagct gttttttgaa attgttcctt gtgactcgga
    32401 gatgaacggc aggctcacga cctcttggaa aacggtgttc aaaagcttgc atctgcggta
    32461 ataagtccag ccaagttaaa aacataagaa gctattttta cttggctgtt cttattactt
    32521 gatgcagggc agctttttca caacctctca ataaacggtg tgcaatcagc tgaccctcgg
    32581 tattagctca aaaaaaacgg caaaatatga ggaactattt tgccgtttct ttctctaata
    32641 ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc
    32701 aaaataatcg gggtaattat gaatcacgta ttcttgctcc tcgataatca tatgtaaatg
    32761 catcgtgcaa agaaatccga cttcatcaaa gttatgatca atcattgatt gaagcattgt
    32821 ctgatgttca tctaatactc gaccccagtc taattctgaa atcgtcaaac gtaaccatct
    32881 gaaacggttc aagtgcgtac tataactttc tagccaatcc caaatttctc gttttccagc
    32941 aatttcataa caagttcggt gaaaatcagt atccaaatca aaaaaagctt ttttattttg
    33001 agcgattact gcctgatcgg tttttccaac ttttcccgcg tataacgggc taatgaagca
    33061 gatcggatat cgatttttga aacataagtg ccggactgtg gaatggtttt tacaagctct
    33121 tgattgcgca gttggatcag tgcttctcga atcggtgtac gcccgatagc tagttgttct
    33181 tctaacactt tatcggagat tttacttcca ggtgctaact cggaataaat gatttgttta
    33241 cgtatagatt cataagcttg ctgttgcaaa ttattgataa ccatttaacc ttcctttcta
    33301 tgcagatata atctattata acataaatcg cttacaaatc gtgtgggcat aaaaacacag
    33361 tctgcaaaaa aagccctgat aggcatgcct attagagcct cgaaaagaaa aatattgaat
    33421 caatctggtc gtacctcttg tatcggtttg acacgaccgc cgattctttt agcaaaagct
    33481 tcggattctt ttttgtcttt gaaaatgagc agattgtcta agtttttttc ccgagtgcga
    33541 cagccagttt tatcgatgta accgtgcatt actttaacga catacatggg gatcacctgc
    33601 ttttttcttt tcattatacg aactactcga cacaatacaa gaaaaatgct tagacggtct
    33661 ttattaagaa tttacatcat ccaaagaagc gtacgcttgg aataactgat ctaactccgg
    33721 attgactttg ctcagcttca ctaatcgttg cgaatcagaa agtaaaaaac agtgtttact
    33781 actgccagta gtttttaatt ttttgtcttc agttcccaca atttcgtagc taaaatccaa
    33841 ccgtgtcccc gtatactttt ggatagttgg atggatgctc actttttctc cgtaatggat
    33901 catttcttta tagctgcaat ttacttccag aacggggatg atgatcccag attcttcgat
    33961 ttttttatag ggaaacccta gatgtaccaa taaggctacc cgagcctctt caaaccagcg
    34021 gatataattg ctgtgatgaa taatccccat ttgatctgtt tcataataaa aaggttctcg
    34081 cacataacct gtaaatttct ccatgactca tgcctcgttt tccattgatt ttttacaaaa
    34141 ataattcatc catttcggaa gtgcttcgct gatttgtgtc ggcaaaacaa tatattgttc
    34201 ttttgccagc tggtctccaa tgaaactatg aagataaata gctgctgcaa tcgtttggtc
    34261 attttgagga aactgggcta aaaatcctgt gatcatccca gctaaagtgt cacccatgcc
    34321 gcccgttgcc atagcagcag aacctaaagg attggtgtag aagtgctggt gaccataaat
    34381 ttttgtctca tgacttttca aaacgagcac agcaccgatt tcctgttgct tctttttgtt
    34441 ttctttatcc cgttgttctt gtatcggtag agaactcaac cgttgccact ccatttgatg
    34501 aggtgtaagt acagtttgtt cgggataggg tagcaaaaga tgattagcag caagtaaact
    34561 taaagcagag ccatcaatca ccagccattg ttcttttgat tggttttcta gtacttgttt
    34621 gaaacgttcc aaactcttag aagaagtacc caatccggga ccaatcaaga gtacatctgc
    34681 ttgctttatt acggaaagaa actggtcagt atccgtccaa actacaatca ttgcttctgg
    34741 cagatgactg tgaagagcac tccgattttt ttcatcagtt actacagtcg tgagacccgc
    34801 tccgctgtgt acggcagcta aagcactcat aatgattgct ccgccatatt gttcattgcc
    34861 gccaatcaaa acgatacgtc caaatgttcc tttatgtgaa atttttggac gaggtgtgat
    34921 gactcttttc actagttgtt cgtttatcag catctaaaaa cctcctttta ctcattatag
    34981 cgtatatttt ttttagagaa aaacagataa gaaaaggaat tgtcagattt tttgaactat
    35041 ttttttctgt aatttgtggt atcatgaaca cgaagactta aataagtgga ggattaactt
    35101 tatgacaatt gattggcaaa aagaagtaga agcacgtaaa gaagacttgc ttgaagactt
    35161 gaaaaatctt ttacgtgtca acagcgagcg cgatgactca aaagtaacac cagatgcacc
    35221 atttggacct ggacctcgcg acgcattgaa acatatgtta gcttatggcg aacgtgatgg
    35281 atttaaagta aaaaatgtcg ataactatgc agggcatatt gatcttggcg aaggagacga
    35341 aacgctaggg atatttggac acatggatgt cgttcctgca ggagatggct gggacacaga
    35401 tccttatgaa ccagtaatca aggatggaaa gatctttgca cgcggatcaa gcgatgacaa
    35461 aggtccaagt atggcagctt actatgcgat gaagatcatt aaagaacttg acttgaaact
    35521 atcaaaaaaa gttcgcttcg tagtcggcag cgatgaagaa agcggctggg gcgatatggc
    35581 ttattatttc gaacatgaag aagaaccaga ttttggtttc tcaccagatg ctgaattccc
    35641 aatcatcaat ggtgaaaaag gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc
    35701 aggagattat gtcttgaaat cttttgtttc cggacttcgc gaaaatatgg tgccaggaac
    35761 agcaactgca gcacttcaag taccaagtgc agatgcagct atcgcgatgg aagaagcatt
    35821 ttaccaattc atcgaagcaa atccagtaag cggaacgatt gaagcggaca atacgtatgt
    35881 aaaaatcgaa ttagttggta aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc
    35941 aggttctttc ttggcattgt tcttagataa ctatgaattc ctaggatctg ctaaacaatt
    36001 tatccatgta gcagctgctt atgtccatga agatttttat ggagaaaaac taggagtcgc
    36061 ttatgaagat gaaaaaatgg gcaaattgac aatgaatgct ggactatttg cttttgaaga
    36121 aaatggtaca gaggaagcga acttcatcaa tatgaatttc cgttttccaa aaggtgtaac
    36181 agtagacgga ttgcaaagtg acatcgaaca aacagtgggg caagaaggcg caacagttac
    36241 tcgaggtgct cgcgtaatgg aaccacatta cgtaccaatg gatgatccat tggtggctac
    36301 attattgcaa gtctatgaag accacactgg agaaaaaggc tacgaacaaa tcatcggtgg
    36361 cggaacttac ggacgtttgt tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata
    36421 tactgataca atgcatcaag caaacgaatt tatgagtctt gatgacttgt tcagagcaac
    36481 agcaatctat gcagatgcca tctatcgttt ggcgaaataa ttaattaaaa agaggatcaa
    36541 gaattgcaaa ttatttttgc agtcttgatc ctttttttac tttctactgt ggtattcatg
    36601 ataaacatga tatagattca attcatattc tttcttagcg ttcgtgacga ctgtatccaa
    36661 aatcaaccct gttactaatg aaagaagtgc taaagtcatc aatccagtac ttaaaatcgc
    36721 agaagggatg cgtgtaataa agcttgtcag catatattcg cgaatcacag gaatccccgt
    36781 gaccaatcca aataaaaaga agaagatggt ccaaatccca aagaaaagca aaggcttata
    36841 atctttaaac attttaataa tcatcatgat cactttgaat ccgtcagaga atgtattcaa
    36901 ttttgattcg ctgccttcag gacgatcacg ataatcgatt gggatttcaa cgaacttgaa
    36961 ttttttatct aaagcatgga tcgtcaattc cgtttcgatt tgaaaaccag aactcataat
    37021 aggaaaactt ttgacaaaga tacgattgaa tcctcgatag ccggtcatga catcaagaat
    37081 ttttgtttta tacattctag taattgtatt tctaacaagg ttatttccaa aatcgtggaa
    37141 aggtctctta ttctcatcaa agtaggttcc atttgatagg cgatcaccaa tgaccatatc
    37201 tgcttcacca gaacgtaatt tctcgagcaa accatgtaca gcttctgcag gataagtatc
    37261 atcgccatct accattaaat aatagtcggc atcgatatca aagaacattt ggcggatcac
    37321 atttcctttt ccttgacgag gttctttttt tacgattgct ccaccttgaa cggccaattc
    37381 gtatgttcga tcggtcgaat tattatcata gacataaata tctgcttctg gtaattctcg
    37441 tttaaagtct gcaatgactg ttgaaattgt tgcttcctca ttgtaacaag gaatcagaac
    37501 agcaattttt tcttgttgtt ccatttttca ctctccttaa ctatagaaaa caaaagctaa
    37561 attaaaaata cctcataatc aaaggaaatg ctagtttttc ccgctaaata tcatttattt
    37621 aaaaagaaaa tactggaata tatgtttgga aagctctatc taaaaaacca cccactacaa
    37681 aagaaggaca tgatacctct cttttgaata gtgggtgcgt attcgttctc tcactttttt
    37741 gacgtattaa tctgttgcac ctttttttct tgaaatttct aatacggttc ctgtatgagc
    37801 atctgctaaa aattcataat aaacagtttc gccatcttct attcgagaaa gacctccccg
    37861 ataagcactc gaccgaacgg cgaatttcct aataggttcc ttttcaaaag agatccaaga
    37921 accttcgatt gcaccttctt ttagaaaaga tgacttgata ttttcaagta cttggtctgc
    37981 tgacaatact tttttgcgat gataccaaat cgttgaagct accccgctca ataatcctag
    38041 accgattcca actgctaacc cgcctttaaa atagcttagt tcgctttctt catacatgtg
    38101 gacatacctc cttcttctca cttgttactc cttgtgctta ttttaccaca ctctcatatt
    38161 ttctttgaaa taaataaccg ctaccagaaa aaataagcta taataaatac gatcggtctg
    38221 cagaaaaaat catattttat ccgcagatca atataagtct gagaggagtt tagtctgaaa
    38281 tttagacgaa tagcatatgg aagaaaaaac atttcaacga atcaaagagt taacagaatt
    38341 acaaggaaca agcggttttg aacaagatgt tcgcgcttat atggaaagtc atatggaacc
    38401 gctggtagat gagttacagt tagatggttt gggcggcatt tttggtctgc gccatcatga
    38461 agaagcggat gcaccccgtg taatggtcgc agcacatatg gatgaagtcg gattcatgct
    38521 gacacaaatc caagaaaacg ggctttttaa agtcgtacct ctaggcggct ggaatcctta
    38581 cgttgtttct gctcagcgtt ttacacttaa aacctctact ggtaagaatt atccatgtat
    38641 ttcttcttcc gttcccccac atttattgcg aggaacaagc ggacaaaaat cagtagaagt
    38701 aaccgatatt ttgttcgatg ctgggtttga atcaagagaa gaggcaatga gttacggtgt
    38761 ccttccagga gacacaatcg ttccttacgc tgaaacgatc aaaacagcca acggaaagaa
    38821 tattatcagt aaatcatggg acaaccgcta tggctgtaca atggtattag aagctttaga
    38881 agcgttgcaa aatgaaacat taggtcatac attgattgca ggcgcgaatg tccaggaaga
    38941 agtaggcttg cgtggttcta aagcttctgt caataaattc aagccagatc tgttctttgc
    39001 agtagactgc tctgcagcag atgatacagt gacgaaaaat ggcacattcg gacatttagg
    39061 agaaggtaca ttgatgcgta tccaagatcc aggtttgatc atgcttcctc gtttgcgcga
    39121 gtacttgctg gatatcgctg aaacaaacaa tatcccttat caatactttg tttcaaaagg
    39181 cggtacagat gcaggtgctg ctcatacaca aaacgaagga atcccaagta cagtgattgg
    39241 cgtagtggga cgttatatcc atacgcatca aacgatgttc agtattcgcg attttgaagc
    39301 tgcacgagaa atgttgattc aaacactgaa aggtttggat aaatcaacag tgaatacgat
    39361 cgtatacgga aaataatgcg atagtgaaac tataaaatct ggcaaaagat tgctttttgc
    39421 cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag gtgtataatt
    39481 taattgaaaa atacataaac agaaagtaga ggacaaagaa tgattatccc aaatagttta
    39541 gaagaattag caggctacgt agaaactgga aagagtgtat ttttctttac tgccggctgg
    39601 tgcggagact gccgttttat caaaccgcaa atgcctgaaa ttgaaaatga cttttctgat
    39661 tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga atggaacatt
    39721 tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg tctagtgaat
    39781 aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaac aaaaggctga
    39841 catctgttag gaggggtcaa gatgaatcaa gaatataaga atattttagt aggtatcgac
    39901 ggtagtgaac aagcacttgc tgctttcaaa aaagcggtag aagtggctcg ccgtaataat
    39961 gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataactttat gggctatgca
    40021 cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaaggaaat gatcgaagaa
    40081 tgtaaagaat atgggaaatc tgttgattac acaaatatag aaggaattat tgcatatggt
    40141 tctgttcgtg aatccatggc agtcaaattg ccaaaaaaat atgagatcga tctaatcatg
    40201 gttgggcagt caggtctaaa tgcagtcgaa cgctttatga caggaagtgt agccagctac
    40261 gtgatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga acagccaagt
    40321 aattaagcaa gtggaacttg tacgaccttt ttaatcaatt aactaaaaca gtaaagggac
    40381 tgtgacaaaa gtcttgtcac agtcccttgt tacgaataaa tggtgttcca gaagaggctt
    40441 cttcggaaat aaagcatgaa aaaaatttca tgcagtgaat gcaatttttt gttaagataa
    40501 atctcagcaa aggataatgg aggaaagaac atgatttttg cttataacaa agcacacgtc
    40561 ggagacacct tgatggttat tatagccgac gataaaggaa cagaaaacac tgttgaacgc
    40621 aaatggaatg ttgctcggat caaagatgaa caaggtcaag ttgttgcttg gaactttttc
    40681 catatctccg atcatctaac aatcgaaggg aacggccaag taagcttaac agatgaggaa
    40741 ttagcgaaat tgaatcgatt gatcaaagaa gcaggattcg aagaacagct tgaagctgat
    40801 catgaaccga agatcgttgt cgggtacgta aaaacatgcg tcccacatcc tgattctgac
    40861 catttatcga ttactgagac agaagtagac aatggtacag tattgcaaat cgtttgcggt
    40921 gcacccaata tcgaagctgg acaaaaagtt gtcgttgcaa aaccaggagc tatgatgcca
    40981 gatggaatga tgatctggcc aggaactttg cgaggggtag atagctatgg aatgatctgt
    41041 tccgctcgtg aattgcattt gccaaatgct ccagcaaaaa aaggaatctt agtattgcct
    41101 gaggatgccg tagtcggaga agcatttcca aaataaagag gttgtgacaa gtctttgtca
    41161 caacctcttt tttattaatt ttcactattt tgattttgtt ttaatgccga ttgatcgatc
    41221 gtcaattcaa cagtagttgt tttttctttc gagccttcat agtaagtgat tttgacagaa
    41281 tctcccactt ttttcttata caaagcagat tgaagttcta caccagaaga aacttctttg
    41341 tcatcgatct tagtaatgac gtcgtattgt ttcaatccag ctttttcagc cggtgtagct
    41401 gtttggacat ttgtcacgat gacaccgtta gttacagatg aaggaatctt caagatttgt
    41461 tcttgttgct gagtagaaac ggcagataag tcgaccattg tgatacctag agctggacgt
    41521 gtcacttttc cgtctttttc cagttgattg atgatattca ctacatcgtt acttggaata
    41581 gcaaatccca ttccttctac gcttacatta gaagtagatt cagaagtact tgcgatctta
    41641 cttgagttga taccgataac ttgtccttcg atattaacta aaggaccacc agaatttcca
    41701 gggttgattg ctgcatctgt ttggatcgca ttgatattga ctgtttcgtt cgattcattc
    41761 gtacttgtca cttgtcggtt caaggaagaa atgattcctg aagtgacaga atttgcatat
    41821 tcagatccta gtggggaacc aatcgcaatc gcaggctcac caaccttcag agcacttgag
    41881 tcaccaaaag aggcaactgt ttcgactttg tctgaattga tcttcaacac cgctaaatct
    41941 gaataagcat ctgttccaac taattcagct tttaccttcg taccgtcttt catcaagact
    42001 tcaagacctt gctggccatc tactacgtga ttattcgtga ctacataagc agtattcccg
    42061 tcttttttgt agatcacgcc gcttccttca ctataagctt ccaaattact atcatctgag
    42121 ctgctacttt cttcttgctg cccgaatagt ccgccaaaac cgcttgattg gttttgactt
    42181 tgtaagttga taacagaaac gacagcgtct tgaactttat ctaccgcttt ggtgatatca
    42241 gaatctacat tgactttgac attttccaca actgtttcac cggcagaatt ctgattgccg
    42301 cttgtagcag tagaactatt gttgccagtg cccatcgctg cataaaaaat gccagctgtc
    42361 agtagtcctc caacgatccc tccaacaagc cccagaccta acttacgcca gaggctatta
    42421 tttttacttt ttttcatctt tggtgtcaca ttttttctat ccatgtgttc ctcctccaat
    42481 tacattcatt tatctactgt tagtatagtc ttatttgttg actttagtct actttgagac
    42541 tgtgaacttt ttatgaaaaa ctttaggaaa aatcattgtt ttcagatgaa aatatttgat
    42601 tttctttttc cacagatact gtgtaaaact acgcggatag gattggaaaa ataagggata
    42661 aaacatatcc acagcctgtg tataaagtgg ataaattctt ataaaacgta tgtttgccta
    42721 atgaatgtga tgaaataagg taaaataaca ctgttttctg tggataaacc tgtggatgtt
    42781 gtgcataact gttggggaaa tctttttaac aaaaatagcc aaaataaaca tatagacgaa
    42841 aaaattgatt ataataaaca aagtgagcat gaagaccaaa ggagagaaaa catgaatatc
    42901 aaaattatct cagtaggaaa attaaaagaa aaatacctga tccaaggtat caatgagtat
    42961 gtcaaacggc tgaatgccta tgcaaaaatc gaattgatcg aagttcctga cgaaaaagcc
    43021 ccggagaatt taagcgaagc gcaaatgaga caagtaaaag aaaaagaagg ggaacggatc
    43081 ttagcgaaaa tcaaagaaca agaatacgtc tatgccttag caattgaagg gaaaaatcct
    43141 acaagtgaat cttttgctaa acaaatcgac caattaggta tccaaggaaa aagccacctt
    43201 gtttttgtta tcggcggctc tttaggactc agcgaagccg tcatgaaaag aagcaatgct
    43261 cagatctctt ttggaaaaat gacgtatcca catcaattga tgcgtctgat ccttgtcgag
    43321 caaatctacc gtgccttccg gatcaatgcg ggggcgcctt atcataagtg atgcggtttt
    43381 tatcattgat cctaaaaatc ttagcaagac aaaaagtacg taagaaagta aagactaaat
    43441 acttcggagg aacaaatgaa taacaatgat aggttattgc gattgcgtta tgcgattgat
    43501 ttgaaagata cggatttgat taaagctttt gagctaggcg gtgtaccact gactaaagaa
    43561 gaagctcagg cggtactgac caaagtacag gataagaaca aagataatgc agaaaataat
    43621 gtgtatgaaa aaaccatcaa caaccaagtt tttgacgcat tcctaaatgg cctgattctc
    43681 ttggcgcgtg gtccgcaaaa agataagccg atagtagaca cgccgcaaaa aagcaaggaa
    43741 atcaaatata tcaacaatgt gttgctaaaa aaattaaaaa tcgccttgtc tatgaccact
    43801 gatgatattt tagacgtttt tgctgaggca gaaatctatc catcaaaagg cgaaatcggg
    43861 gcatttttga gaaaagaagg ccaacgaaat tttaagcctt gcggagataa atatatgcgt
    43921 aattttctca aggggttagg aatttataat cggagaaaag tataaagaaa tttcatgaga
    43981 aatcaaaaca atggttgttt tgatgagagc tagtttttgt atgagattaa aatattatct
    44041 taatcgagga gctggattgg ttttatgaat gatgaatata agaatgatga agataagatg
    44101 ctttttgaag agatagagaa tcgatgtagg ctcaattttg agcttcgggg aaagatgtct
    44161 ttaatacaac aaaaaaagta tttagcgaat aaatcagagt ttactctggg acatgttgaa
    44221 aaattaatct cggattggat tagtagtaga agcgaattta caaaaataaa acagccgatt
    44281 aaatttgaca tgaaaaaact tttgttaaat aagagtgaga taggtaatag agatcaatat
    44341 attagagcta aagggcaaga aataattgat tcattaggtg aaatgaggtc atacaattat
    44401 ttgtatgtaa cacatagagc tgatggaatg gtcattactg tggggaaatc ttcttctaat
    44461 gatatttttt tagatggaga tcttttttat caactcaata caaatcactt gtctggcact
    44521 gaaaatataa ttttaaggac ggaatatggc aatgaaatat ttgcaaaata tgatgagatt
    44581 ttaaaaaatt atttggactg ggcttggata attccggttg agtcaggaga tgctaagaaa
    44641 ctggagagat tattgggcga tgaactaatt aataaaaaag taccaatttt aaattattat
    44701 tcccatagac aataaaaggc tatttttcat gataatacta gttataactg taaaaatgga
    44761 agaaaatctg ggaaagaagt ttttaaataa gtgtattgtt cgattttaat aaataataag
    44821 caataccatc cgtctgtgtt gcgcaacatc gtactacaat gaatattttt tcacaaaaag
    44881 aaaatcatcc atcgaatgaa tggaaggttt tagtatacac gaggttaatg ccccctcttt
    44941 tataatcaga ggagtgaggt tttttctatg aatgtttcta ttttcttatt gctagtctag
    45001 tttctcttac gatgaaattg ggattctatt ccagttttta ttgatttgct gataaaataa
    45061 cttgaatggt ggatataaac atgataaata agagaagaat aacaaaacaa ggttgtgaaa
    45121 tgattctcaa atgaatataa tagaaagaaa aaggaggaag aatcaatgaa aatagcaatt
    45181 gcaggcgctg gcgcaatggg aagtcgcttt gggttaatgc ttcaccaaag tgggaacgaa
    45241 gtattattaa tcgatggctg ggcagaacat gtccaacaaa tcaaagagca tggattacaa
    45301 gcaaatttta atggtaaaga ggtagaagca aaactaccaa tcgttcttca atccgaagta
    45361 gaaaaagaag atcaagttga tctgattatt ctatttacca aagcgatgca gctggaaaaa
    45421 atgctgcagg atatccaatc attaatcaaa aaagatacag aggtcttatg tctattaaat
    45481 ggtatcgggc atgaagatat tattgagaaa tttgtaccaa tggaaaatat ctatattgga
    45541 aataccatgt ggacggcagg tcttgaaggt cctggtcagg tcaaattatt tggaagtggt
    45601 tcagtagaat tacaaaattt aggtgatgga aaagaagcag ctgcaaaaaa attagcagac
    45661 aaattgtctg aatcaggatt aaatgctcat ttttctgaca atattcacta ttctatttat
    45721 cgcaaagcat gtgtgaacgg aacaatgaac ggactatgca caatcttaga cgttaatatg
    45781 gctgagctag gaaaaacatc gactgctcat aaaatggtgg cgacgattgt caatgagttt
    45841 gccaaagtag cagcagtaga gaagattgaa ctagatgtcc cagaagtcat tgcacattgt
    45901 gaatcttgtt ttgacccgga aacaattggg ttacattatc cttcaatgta tcaagacttg
    45961 attaagaacc atcgattaac agaaatcgat tatatcaatg gcgcaatttc tagaaaaggg
    46021 aagaaatatg gtgttgcgac gccttattgt gatttcttga ctgagcttgt ccatgcaaaa
    46081 gaagatagtt tgaacgtaaa ataaaggaga gattgaatgg aaacttcaca aaaaatatca
    46141 ccgaaaattt ttctgaataa agtcctcgct ggtacagcaa caggaatcat tattggattg
    46201 attcctaatg cagtattagg tgccatttta aaatatttta gtgcgtatcc ctttgcggta
    46261 acgatcgcac agttagcagt tatttttcaa ctagcaacac ctttgatcat tggtggtttg
    46321 attgccttac aatttggatt tgatccgatg agaatgatgg ttgtagccgg agcttctttt
    46381 gttggttctg gtgtagtcaa attcacacct gatttgggtg aaacgggtgt ttacgttggt
    46441 gctggtacag gcgatattat taatacgatg ctgacagcat cgattgctgt cgggctgatt
    46501 ttactgattg gaaatagttt tgggtcagtg tcgattgtgt taacaccgat tgttgtaggt
    46561 gtaggtgcag gtttacttgg tttttattta ttcccttttg taacagcaat tacttctgca
    46621 attggatcat tgattaataa cttcactacc ttacaaccgc tgttaatgtc tatcttgatt
    46681 ggttgttcct ttgccttttt gattatctct cctatttcaa cagtagcaat cggaatggca
    46741 atccaactag atggtgtttc tgctggtgcg gctgcgatgg gcgtagctgc aaccactttt
    46801 gttttagtag tgaactcttg gaaggttaat aagtcaggtg tgacaattgc gattgcttta
    46861 ggcgctatga agatgatgat gcctaatcta tttagaaaac ctgtgattct tattccttgt
    46921 ttagttagtg cagtgattac agctattcct gtcgctctat tttctatttc aggtacgcct
    46981 gcttctgctg gtttcggcgt ggtcggactt gttggtccgt tggcttcttt agatgcaggg
    47041 ctaaatggct ttcttgttgt tctttgttgg ttagtcatcc ctgtttttgc ggcactaatt
    47101 actcaatttt tatgcgagaa agtatttaaa ctgtatgatc gagaagaagt cttcaaattt
    47161 ttaggctagt agattttact aagaaatttt caatcaatgg atgcgtaagt gagatgtcaa
    47221 caacatctga cttacgtttt tttgcctttt tgagtaggag attgatgctg acaactgaac
    47281 tcttcagtgc cattcgattt tttaaaatgt agaagaaact cacctttatg ttattgattg
    47341 ttcaaaaggc aactagaata aaaatatgtg ttaaaatgag aaaaaatttg attgttggat
    47401 gtatcgaata atcactaaaa agtaggagaa gaaaatgatt tttttgcaaa aatatggttt
    47461 ttatttttta ttattaggtg tgattagtga tttatcgaca ccgtatatcc ttggcctttt
    47521 ttatcccaaa ctgaatcaaa tgactaccgt aattagtgtg tttggtgacg tagatagtcc
    47581 agtcagacgt gctttcttgg tctggtcagt tgtatctgga cttttttttg ttctttcgtt
    47641 gcctgcgttg tatcacctat ttattggaac atcaaaaaca cttgcaatac ttgctgttgc
    47701 aacagttggt ttatatggga taggtgattg tatttttact gggttattca gtatcaatac
    47761 gaatgaaagc tcttggaatc tatcgacttg gatccataat ataggttctg ggctaggcta
    47821 tgcaggcttt ctacttttcc cattgttact tgttctgctt tatcgtcaaa gtggtcaagg
    47881 tacgcttagc catttttatc ttgttttaac agttattagt ttactaattg caggtcttta
    47941 cggattagca cggattccgt cgattagtca gtttgccttc tttaagcaat tgggcttctt
    48001 tcaacgctta agttttttct ttaactattt gccgatgatt tgtttaagtt tttttcaact
    48061 acgaaagtag aaaaagcagt gtgaagctga ttaagtaaaa gaatttctct atgggtaacc
    48121 attcgtgtgg atgtgttcga ctaagaatag agatgaggag agcgaaaagc aagttagtct
    48181 taacttgtta tgcgttgtgc taaatttcag gtagtcattt tttgaatgaa taagaagaaa
    48241 acttgaaaga gggtgacttc atgttagatg tggagcagat ggaaatagac ctttataata
    48301 ttattttaaa tcgaaataca agagaatggg aaagaacaca atttgaagag gcaaaagaag
    48361 acctagcaaa aatgtcgata aagcaggtga tagaaaaact agaggtcaac ttgcgtccgc
    48421 tagcactacg agataatttg acaccagatg cgatggattt ttatttaaga atcacagggg
    48481 agtcagcgag tgcaaaggct tatgattatg ccaaacataa gataacttca cccttatacc
    48541 aagaacgggc gatttttgcc ggtggctgtt tctggtgtat ggttgagcct tttgaaacaa
    48601 aaccaggtgt aatctcagtg ttatctggtt atacaggtgg agagaccaaa aaaccaaatt
    48661 acgatcaagt cagtggtgga tatactgggc atgttgaagc tgttgagatc atatttgata
    48721 cacgtcaaat aacctatgaa gagctgttgg agatttattg gcaaatttca gaccctacag
    48781 atgcctttgg tcaatttcaa gatcggggga aacaatatcg ttcaattcta tttgttgtta
    48841 atgaaaagca aaggcaattg gctgaaaaat caagagaaca actgattcaa tctaaccgat
    48901 ttagccgtcc tatcgttaca gagattcgcc aagcaaagac tttttggcca gctgaaaatc
    48961 atcatcaaca attttatcag aagcaaccta aaagatataa aaagatcaag cattcgcatc
    49021 agcttcattt attttttcag cgacatagat agaggaaggt tgtggcgcaa agcgttcagc
    49081 tccgacaaaa taatgagcaa ctgacaaaaa tagcttttca tatttttgga cagttatgaa
    49141 ttatttcgag gagttgcttt gagaacaccg aggaaaaggt tgtggcgcaa agcattcagc
    49201 ttcaagtcgc aaggaaacag gaggtatgac aagtcttttg tcacaactcc tgttttttta
    49261 ctagccttta tttccatgtg aaagagaatt cgaattgtag aatcttgatt tttctatgga
    49321 attttgtaga gaatcatctt gataattaat agacacctaa tcgaattcca cattaatgat
    49381 gaactaaatt ccgtataaaa gcgcaaacaa taacta
//
LOCUS       DAZUKT010000012        45071 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000012 DAZUKT010000000
VERSION     DAZUKT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.012"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1986
                     /locus_tag="ACG816_000680"
     CDS             142..1986
                     /locus_tag="ACG816_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919592.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            complement(2031..3035)
                     /locus_tag="ACG816_000681"
     CDS             complement(2031..3035)
                     /locus_tag="ACG816_000681"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4919593.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3046..3786)
                     /locus_tag="ACG816_000682"
     CDS             complement(3046..3786)
                     /locus_tag="ACG816_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4919594.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            complement(3856..4659)
                     /locus_tag="ACG816_000683"
     CDS             complement(3856..4659)
                     /locus_tag="ACG816_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4919595.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            complement(4659..5741)
                     /locus_tag="ACG816_000684"
     CDS             complement(4659..5741)
                     /locus_tag="ACG816_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4919596.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            complement(5882..7150)
                     /gene="dltD"
                     /locus_tag="ACG816_000685"
     CDS             complement(5882..7150)
                     /gene="dltD"
                     /locus_tag="ACG816_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4919597.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(7153..7386)
                     /gene="dltC"
                     /locus_tag="ACG816_000686"
     CDS             complement(7153..7386)
                     /gene="dltC"
                     /locus_tag="ACG816_000686"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4919598.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            complement(7431..8636)
                     /gene="dltB"
                     /locus_tag="ACG816_000687"
     CDS             complement(7431..8636)
                     /gene="dltB"
                     /locus_tag="ACG816_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4919599.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(8633..10147)
                     /gene="dltA"
                     /locus_tag="ACG816_000688"
     CDS             complement(8633..10147)
                     /gene="dltA"
                     /locus_tag="ACG816_000688"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4919600.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(10158..10307)
                     /locus_tag="ACG816_000689"
     CDS             complement(10158..10307)
                     /locus_tag="ACG816_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4919601.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            complement(10588..12630)
                     /locus_tag="ACG816_000690"
     CDS             complement(10588..12630)
                     /locus_tag="ACG816_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919602.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            complement(12620..13387)
                     /locus_tag="ACG816_000691"
     CDS             complement(12620..13387)
                     /locus_tag="ACG816_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919603.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            13713..15902
                     /gene="nrdD"
                     /locus_tag="ACG816_000692"
     CDS             13713..15902
                     /gene="nrdD"
                     /locus_tag="ACG816_000692"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4919604.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16217..16819
                     /gene="nrdG"
                     /locus_tag="ACG816_000693"
     CDS             16217..16819
                     /gene="nrdG"
                     /locus_tag="ACG816_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4919605.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(16873..17994)
                     /gene="dinB"
                     /locus_tag="ACG816_000694"
     CDS             complement(16873..17994)
                     /gene="dinB"
                     /locus_tag="ACG816_000694"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4919606.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(18103..18975)
                     /gene="rsmI"
                     /locus_tag="ACG816_000695"
     CDS             complement(18103..18975)
                     /gene="rsmI"
                     /locus_tag="ACG816_000695"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4919607.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            complement(19044..19391)
                     /locus_tag="ACG816_000696"
     CDS             complement(19044..19391)
                     /locus_tag="ACG816_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4919608.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            complement(19384..20208)
                     /locus_tag="ACG816_000697"
     CDS             complement(19384..20208)
                     /locus_tag="ACG816_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4919609.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            complement(20244..21182)
                     /gene="holB"
                     /locus_tag="ACG816_000698"
     CDS             complement(20244..21182)
                     /gene="holB"
                     /locus_tag="ACG816_000698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4919610.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            complement(21196..21525)
                     /locus_tag="ACG816_000699"
     CDS             complement(21196..21525)
                     /locus_tag="ACG816_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4919611.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            complement(21540..22184)
                     /gene="tmk"
                     /locus_tag="ACG816_000700"
     CDS             complement(21540..22184)
                     /gene="tmk"
                     /locus_tag="ACG816_000700"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4919612.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            complement(22248..22844)
                     /gene="recR"
                     /locus_tag="ACG816_000701"
     CDS             complement(22248..22844)
                     /gene="recR"
                     /locus_tag="ACG816_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4919613.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            complement(22869..23183)
                     /locus_tag="ACG816_000702"
     CDS             complement(22869..23183)
                     /locus_tag="ACG816_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4919614.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            complement(23203..24948)
                     /gene="dnaX"
                     /locus_tag="ACG816_000703"
     CDS             complement(23203..24948)
                     /gene="dnaX"
                     /locus_tag="ACG816_000703"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4919615.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            25163..26410
                     /locus_tag="ACG816_000704"
     CDS             25163..26410
                     /locus_tag="ACG816_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4919616.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            26435..27619
                     /locus_tag="ACG816_000705"
     CDS             26435..27619
                     /locus_tag="ACG816_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4919617.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            27616..28413
                     /locus_tag="ACG816_000706"
     CDS             27616..28413
                     /locus_tag="ACG816_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4919618.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(28855..29511)
                     /locus_tag="ACG816_000707"
     CDS             complement(28855..29511)
                     /locus_tag="ACG816_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4919619.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            29704..29982
                     /locus_tag="ACG816_000708"
     CDS             29704..29982
                     /locus_tag="ACG816_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919620.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            30001..31209
                     /locus_tag="ACG816_000709"
     CDS             30001..31209
                     /locus_tag="ACG816_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4919621.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(31357..31722)
                     /locus_tag="ACG816_000710"
     CDS             complement(31357..31722)
                     /locus_tag="ACG816_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4919622.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(31869..32642)
                     /locus_tag="ACG816_000711"
     CDS             complement(31869..32642)
                     /locus_tag="ACG816_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4919623.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(32816..33676)
                     /locus_tag="ACG816_000712"
     CDS             complement(32816..33676)
                     /locus_tag="ACG816_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4919624.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(33676..35094)
                     /locus_tag="ACG816_000713"
     CDS             complement(33676..35094)
                     /locus_tag="ACG816_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4919625.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(35151..35600)
                     /locus_tag="ACG816_000714"
     CDS             complement(35151..35600)
                     /locus_tag="ACG816_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4919626.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(36045..36308)
                     /locus_tag="ACG816_000715"
     CDS             complement(36045..36308)
                     /locus_tag="ACG816_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4919627.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(36407..37702)
                     /gene="rho"
                     /locus_tag="ACG816_000716"
     CDS             complement(36407..37702)
                     /gene="rho"
                     /locus_tag="ACG816_000716"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4919628.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(37699..38970)
                     /locus_tag="ACG816_000717"
     CDS             complement(37699..38970)
                     /locus_tag="ACG816_000717"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4919629.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLLTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(39136..40005)
                     /locus_tag="ACG816_000718"
     CDS             complement(39136..40005)
                     /locus_tag="ACG816_000718"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4919630.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            40108..40320
                     /locus_tag="ACG816_000719"
     CDS             40108..40320
                     /locus_tag="ACG816_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919631.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(40297..41907)
                     /locus_tag="ACG816_000720"
     CDS             complement(40297..41907)
                     /locus_tag="ACG816_000720"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4919632.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(42129..42374)
                     /locus_tag="ACG816_000721"
     CDS             complement(42129..42374)
                     /locus_tag="ACG816_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919633.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(43100..44440)
                     /gene="glnA"
                     /locus_tag="ACG816_000722"
     CDS             complement(43100..44440)
                     /gene="glnA"
                     /locus_tag="ACG816_000722"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4919634.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(44468..44860)
                     /locus_tag="ACG816_000723"
     CDS             complement(44468..44860)
                     /locus_tag="ACG816_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4919635.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT        13348 a         9368 c         7845 g        14510 t
ORIGIN      
        1 gctgtaaaaa tgtttccctt taataattat atgtttctaa ttctatttcc tcatctttat
       61 ctcatttttt attataaata attgacgtta gtttaaaaaa cgtttaccct aacattacaa
      121 gttttagaaa ggaatacctt tatgtctttt tttaataaca tatataataa aaaagaatct
      181 ttttatttac gtataaagtg gagcataatc attatattat cattgttatt tttaacagga
      241 attgcttatt tcttttattt tattttttct cctgcggaca gcaatcaaat gaaagaaata
      301 aaagattcgt gggttttcta tacaaaagat gatcctgatt ttaagtttga cagcaagtac
      361 actcgtacga ttcctaccgt ggataaagac gaaacattca ttatggaaac gacacttgaa
      421 aaaccgttgg acgaagcaaa tttactaatc aaaggaaacc atcaatggat tactgtatat
      481 ctaggcgaaa agattttata ccaacggaaa gattatcagg aagaaagcaa tcccggactc
      541 tcattggctg tgatagattt accagcaaat tatatcggaa aaaaattgac ccttacagtc
      601 tcatctcctt atcagaacta tgctggactg ccaccgaaag tttatctagg aacaactgga
      661 ccattgatta gttttattta ttcacaatca gtgccgcaag tattgacgat gataatcgct
      721 gtttttatcg ctatagggac ctttttctat acgatctact tgatgtataa acaaaaacgt
      781 cttgaatact cacttattct cttaagttgc tttgctttgg cattaggatt tgaagctgta
      841 tcagaagata ttttaagtgg tattcttttt gaaccattcg ttcattcttt tttatctcat
      901 ttatttatta tcttaacgtc agctttcatc atctgctact attggagccg aatgttttac
      961 tataaaaaat ggtacggaat tttttgtacg ttccaacttt tgatttttat cggcgttctg
     1021 ctttatgcaa cttttactcc tgcagaactt cctgaattaa tgccgattgc taatattgct
     1081 agtgttataa gcactttagt cacttcactt gcatgcattg gcgaagcata taagaataac
     1141 cgtttttatg tagcttgtac accttggatc gtcttggtag ccattatcca ttgcttgatt
     1201 tacatccaaa cggcattagg tatctatcaa acaacgatca attggtctac gattttatat
     1261 attatcattc taatcgttat tatcagctac aatctaatcg atcacatctt aaataccgac
     1321 aaagatcgga gacaagtaga tttcttaaaa atcaaaaatg atttgttgga gcaacattat
     1381 gagcagcttc gacagcatat ccaagaagta aatacacttc gcctggaatt tgtccaagag
     1441 atggagaatc tgcaagattt aatgcatacg gaccaaacaa aagctaaaaa ctatgtggaa
     1501 aagatattag aagaagcaaa aaatttcgaa atgctgtgtt ctttttgcaa ccaccaggtg
     1561 acaaatctta tcttagcgcg ttaccagaag ttagctgcca aacggaatat tcagataacc
     1621 ttccaagcag atcttcccga agaacttcct atcaacgatg acgatctggc tcaacttttg
     1681 atccacgctt tagagcattc ctttagagaa actcatgcca tcgaaaatcc actgtatcga
     1741 aaaatttatt tgtcgataca cgaacaagag gaacatttcg tcatttgttg cgaacatagt
     1801 gcacattacg atacaaacct atttagtcga ggcatcacag aagaattaga tgatcaggaa
     1861 cgttttgact taatgatgat gaaagacgta gcggatcgtt acacaggaac actaacgcag
     1921 gaaaaagata ctttcattga tcggatcact gttcaactct ctcgaaatta cccgaatcct
     1981 gtataaaaaa tagcggaatc catatatgtg gattccgcta ttttttattt tcaataaagt
     2041 aaggtaagca attcttctgg ttctatcgca ccaaaatatt tcttcacatc aatttggctg
     2101 actatttttg atagagatgc tttatcatat ttctgtccaa ccaatttttt ttccacgtca
     2161 gagatatctc ctagtccgaa gaaatcgcca aagatttttg cttctttgat cattccttct
     2221 gagacattca aatggacttc aatcgaaccg atagaaaaac gtttttggcg agagaaattg
     2281 aaatctggag acttcccgta attccaatcc caatttcgat aatattcctc agagattcga
     2341 ttgatttttt tccaatcttc atcagtcaat tcgtacgttt ttacttcttc tactgactct
     2401 acgccgaaaa ttttcaacaa gatttcttga cgaaattctt ctgttgtcat tttttgcttt
     2461 tcttctggaa gaaatggttt gatattcgtt acacgagaac ggatcgattt gattcctttt
     2521 gattcgattt tgtctttttt tacttttaat gcattgacta cttcattgat atcagagtcg
     2581 aacataattg ttccatgagc aaacatccga ccgttggtag catacatcgc gttgccagaa
     2641 aatttcatgc catcaatgac tagatcattt cggcctttca attccgcccc agcaacaccc
     2701 aattcatgaa gtgcttggat gattggctgt gttactttcg caaagtcacg gaatgaatcc
     2761 ccatcatcag gcatgataaa actaaagttc aggtttccaa aatcatggta gacagctccg
     2821 cctccactta agcgacggac aacatgaatg ccattttctt ccacataatc tcgattgatt
     2881 tcttcaagcg tattttggtt tctgccgatg atgattgaag gttcattgat ataaaagagc
     2941 aaaataggtt catctaaagg catttcttgc agcaaatatt gttcaattgc taaattgatt
     3001 cttgggtcgt ggttttcatt tggtacaaaa atcatgttct tcctcctaaa tgttaaacac
     3061 tttgtcaact gcagcaagtt ctatatcaat ccctttgcct gctgcctttt gcgcttcatt
     3121 tttcaattgt tctaaatcac caaactgagg taggtgtgta agtacaagtt tctttacgtt
     3181 agctgatttc gcaagttctc cagcttcttt tgaagtgaaa tgggcatgat gatgttcatt
     3241 tccttcaaaa agataggtat ctgctagaaa caaatcagca tctttcgcaa aatcagcgaa
     3301 actttccaaa taacctgaat cacctgtaaa tacgaatact ttccccgttg tttcttctac
     3361 gatacgcatg gcgtaacata ctactggatg gattgtttcc ataaaagtaa tcagaaaagg
     3421 accaattttt aattcttcag cttcaaaata aggatgtcct tttgagacat tttccaacgt
     3481 caattcttca aagtgaatgg aatcttttgt gtgtccataa attgaaagca atgtctccgg
     3541 atttctctta ggatgaagtt gccagtaata ctgtaataca ccaaggtcag caatatgatc
     3601 atgatgataa tggctaagga tcacagcatc tagatcaagt ggatcaaggt gttcttgtaa
     3661 tttgatcagc gtagcactac ctgcatcgat caacaaattg aacccgtcag attgaagcaa
     3721 ataacctgtt gtcccttgat ttttataagg atacgctcct aagcatccta aaacagttaa
     3781 tttcatcgta cattccccct caaagattta tcctaccaca aaagttattt ttttgatgaa
     3841 taaactgctt acatgctaag taaaataggc gtctggacct cctttttcaa tccgtgcttg
     3901 ttcataaaat tctgcaactg acacattcac ataaacaatg acccacaaaa gaccgatgaa
     3961 caaagcgatt gctcctagca aataccagcc gataaaagac agctgcaaca aaatatacgt
     4021 accaattcta tctttcaata aaaaccagcc atatttcaaa cattgaataa ccgacaaatc
     4081 aggatagtcg aactttgtcc atgcagcaaa ttggaataaa ccgccaacaa tactcatgat
     4141 gagtgctgaa acaaaaatca tccctatcat cgataaaaca aagctgatat caagaaaatc
     4201 ttctggcatg acacccgtag gtaccgcatt tgaactgaaa agcagctgca tataagtact
     4261 cactccgctg attaaaaaca tgggcagaaa gattaaactg ttgagtagcc agttgatgac
     4321 tgaattgatc aaattgatgc caagtatcga accaaaatac tttctttgga aaactgaaaa
     4381 taaaaggtta ctttttggtc tgccaccccg tacaactact agcgcaatgt aaaacaatgc
     4441 atagttgaat gcgaataaaa gtaaattatc gactagaaaa ctcaataagt gctgctgcat
     4501 gctttcttcc ggaaaaggac tgaaaccgtc aaaaaagttc tggatgacgc tcgtaaatat
     4561 tatagataag aaaacaagcc aagtattgat tccccattga ttttccaaag aaactcgtgc
     4621 ttttttccga tattcagaaa taggtattct attattcatt attcataact ccgaattttt
     4681 tcaactgttt cactatcaag ccgtttcact aattctgtga ctagtttcac tgtattcaaa
     4741 taatcatctt catgaatcac tgatgtatgg gaatgcagat aacgaacagg aacggtaatg
     4801 gctacagacg gaacaccatc tcttgtcaaa tgcatttgtc ctgcatcagt tcctccgcca
     4861 gaaatcactg tatattggaa tggaatatcc atttcctctg ctacatcaat aacaaaattc
     4921 aataattttt tatgcgggat catagatgca tcaaaaatca aaatctgtgg gccttttcct
     4981 aaaatcgaat ctgcttcttt tggtgtcatt cctggagtgt ctcctgctgt accagtatct
     5041 aaagcaaaag caatatcggg atttacaagg tgtgtacttg ttcgagcacc tcgcaagcct
     5101 acttcttctt gcacatcgct tcctgcgaat aaaacatttg ggtgtgcttc tttagataga
     5161 ttctctaata ctcgcagaga aacagctgtc ccgatacgat tatcccatgc ttttgccagt
     5221 aaatatttac tgccattcat tcgtttgtat tcaatatatg gagtgaccat atctccagga
     5281 cgtatgcccc actctttggc ttcttcttgg cttgatgcgc caatatcaat gaacatatcc
     5341 tttatttcat aaggtttatt tcttacttga ggtgttaaaa catgtggtgg tttggaacca
     5401 atcacaccgt ggacgatttt cccttcacgt gttttgattt caacttgctg agcaagcatg
     5461 acttgccccc accaacctcc aagagtttgg aattccaaga atcctttttc ggttattttt
     5521 gttaccataa aaccaacttc gtccatatgt ccagaaataa agaccttggg gcctccgcca
     5581 gcaccatgtt tggcaatcac actgcctaaa ccgtcataaa aaatatcatc agcgaatggt
     5641 ttggcatatt ctctgaatac ttctcttacc tcttcttcat ttccaggtac tccttttgca
     5701 gaagttaaat caataagtag ctgttcttct tttgtatcca aatgttttcc cccatttcta
     5761 tattcatatt gtgattatac cattgcttat gatttttgtt caggcaaact tggtattttt
     5821 atcttttatt cagaatattc aaaaataaag aaaacaagcg agcaacactc gcctgttttc
     5881 tttattttaa ttgtagttta tctggtattt gatgttgcca agatttgcta aaaaaagcat
     5941 cgtcaagatg atacttacta gcagtaatat gattttcttc taaaaacggt ctgatctgtt
     6001 gatctgcagc caaccagcct ttccagccta gatggatcgt gtcttccata aaatagttgg
     6061 tacccgcttg attgacaaaa tcagctattc gattgaatcc ttggctattt aattgaaatt
     6121 tgatcttttt ggcaaatcct tgtaacattt cttgagatag tccggtataa tcgctccatt
     6181 tctcgttaac cggtgggatg atgaacaata cttcgttatg gttttttgct aattgatcca
     6241 agaccagctg aaaatcagaa aattctggcg aaaaacgata atcccaattt ttttgtgact
     6301 gtttgagctc tggctcgtgg gctcggatac gatgcgtata aaaatcgttt ttcaacccga
     6361 atggattatt cgtcgtttct cgttctccaa tttgatttgc taaaatggat aattctgttt
     6421 cttggtattg ttttggtaat cgttttgact catgatcgat tttttgctgt cgatctttca
     6481 acccaatatt gctgaatact tcatcctctc tcttcagcat gttccattgg ctgcgcagct
     6541 gattcaaaga ttcaggtaaa ggtagcttcc cttttttgat cgtttgcaga atcgccgtca
     6601 acgtatcatt ctcttttact tttgagaaag ttaataatct tctagctaaa tagcgatcag
     6661 caggcgttac tttcttcatt gaaaacaacc agtcgtatgt ttgaagttca gaataatacg
     6721 tattaaagta atcagttttt acaccatttt taacgaacca ctgaggagaa ataataaaga
     6781 cgactttctt gtttttcata gcatctcctg cagaacgcat catcatatat tggcttaacg
     6841 attgtgtccc gggtgctccc agcaaaaatg gacgatagtt tcgctggtat ttttctgcca
     6901 gaacagacgg atgaaatgga ctgattctgc ttaattcact tgagccaaaa aaaggaatat
     6961 attgtttttc agataatgca taatttttta tcgcatctcc tttcaacacg ttcactgaca
     7021 tggaagaaga ggcacgtcta atcgtttctg gattagagat atctacttga taggaggagg
     7081 taaaaaacac aaacagtaag ccaacagcta aaacaaccgg gccgaaaata gcaaaaagtt
     7141 tctttttcat actcaaccct tcaatgcagt tgcttgttga atgattttat tcggtgtgca
     7201 ccaatcctct cgctcgaatt cagatacagg gacttggata tccagctctc cgtctagctc
     7261 gactaataat tgaacagatg ctaaagaatc aagcaaccct tcatcaaata gatcaatatc
     7321 acgatttttt cttacttcat ctgttcctgt tatttcttct aagattgata aaatttgttc
     7381 ttccattttc cttttctcct ttgttatata aattaatatg tcactattcg ttagctaaaa
     7441 aaaagtttat ctaaaaatcc tgaaaaaatc aggaagctaa aacatactgc ttgaaatgtc
     7501 aaaaatacgg ctaagccatg cgtaaatttg ttagaaggca actgctcttt atgcttcttc
     7561 ttaaatcgga tccatgcatc tgtcagacag atcagacaag catgatacag tccatatacg
     7621 atgtagtacc acgtcaatcc atgccatata cccattagaa gaaacaaagc aaaataaccg
     7681 atatttgaca ttacgatctt gcttttaaaa aattttttct tcatcatgaa gaacatcaat
     7741 cgcatatata cataatcacg gaaccaaaag gacagcgtca tatgccaacg attccagaat
     7801 tctttgatat tccatgataa aaaaggttta ttgaaattga ttggtgtctg atatcccatc
     7861 atgtagcttg taccgactgc aaataagcta tagccagcaa aatcaaaaaa caaatacata
     7921 ctgtacacgt acatataacc tactaatgcc catgaaatac caccatgctg taaggcaatg
     7981 cgtcccacta caggaagcag tacttgacca aaataataac cgataatgaa tttatataaa
     8041 aaaccgacaa aaatattttg gacgcctttt cccaaaagat caacatattt atcacgaaca
     8101 ggcggatgga gctgatcttt ttcaaatctc ctatagcgat cgattggtcc agaagagatt
     8161 gttggaaaga acagcagaaa ctggatataa cgaaatggct tatatgtttt gatcatgcca
     8221 tctcttattt ccatgatcat ttgtacagat ttaaaggtta aataagagat tcctaaaaaa
     8281 ccaagtaacg atgcttttcc atgttcaaaa aaaggagtca ctttcgttaa aaacagtgga
     8341 attatcgcta aaataacggc ggcaaaaaac catcctgtct gatttttcct ttgccgataa
     8401 acaaaatagc cccatacaag tatcgtctgc cacaaaacat acccaatcag cgccgctccc
     8461 tgttgccaat aattacctcc aaagctgaca aataaaaaga aaagtgtcac tgctgattga
     8521 taaactggta atcttttttc ccatatcaat aaagatagaa cgatcggtac aagcgcaatg
     8581 gctaaaagaa aaaaataaaa cggctctgca taagggatca tatgaggtaa catcattcag
     8641 gattcacctc agctgttaat ttttttcgat cgatttttcc attaactgtc tgaggcagca
     8701 gatcgacata atggaacttc tgaggaatca tataatccat tacaacttct agtaattgtt
     8761 ctttgataga tttagtcaac tgaaaatccg gctgtttcgt tttgttttct aatacgacaa
     8821 aagcaaccag ctgctgtact tttgttcctt ggtattttgg tacgacgaca gcttgtttga
     8881 ttcggttgtc ttggagcaga taatgttcga tatccccgag ttcaatccgg tatccatgta
     8941 atttgacctg ctgatccata cgtccttcat aaaataaaag cccatattca tctagtctac
     9001 ctgcatcccc tgtatggtaa cttgctaagt cattttgaca aaagaacgca gcagctgtct
     9061 tttcgggatt gttcaggtaa ccttttgata cgcttggtcc tgcaataatg atttcacccg
     9121 tttctccttt tgaaacaggt ttaccttctt ggacaataga aactttcgtg tcttttttta
     9181 cgtagccaat cggtagtcgg tcataactat ctagtacatg ctggtcaatc tgaatacttg
     9241 aaatagctac agttgcttct gttggaccgt aagtattata tatccgtgca tttgggaatc
     9301 gatcaagtag actttctgca gtttttttcg tcaattcttc tccgcaaaat aaaaacatgt
     9361 ccaattccgg cacattttct ccattgaatg taggttccat caagcaaata tccataaatg
     9421 atggagtaga aacccacaca ttcaacttca aggctggcaa tgtcgcgaat aattgacgaa
     9481 agtcttgaac aactgcttta tttaacggct tcagcattcc gccagaaact aatgcaggat
     9541 aaatagaaaa taccgataaa tcaaaagaaa atggcgcttg ggctaagaaa tgcataccag
     9601 gcttgatccc aaagtcttcc atcgtccatt gaacaaagct gactagatta tcatgactga
     9661 tttgtacgcc ttttggttct ccagtagtac cagaagtaaa aataatataa aaattttctg
     9721 taccttttac gctatttctg ggataaaatc ctactggtat cttcttgatt tcttgaaatt
     9781 ctttttttgt tatgacagga accgaaggaa tatcttctat ccagtccccg atactgatga
     9841 ccatagatgg tttagccacc cgaaagatcg ataggatacg ctccttgggt gtatgctctt
     9901 caatcggcaa ataggcatgg ccagatttga caacccctag aaatgagaca atcatttcaa
     9961 actccaaatt gccaaaaacg acaattggtc catcgccttc tacctttttt tctaaataat
    10021 aagcaagtgc gtctgatgct tgtttcaatt caccaaaagt atgcgtctca tccgcctgat
    10081 aagcaactcg atcaggttca tttattcccc aatcatcgat tgcttcaata atcgttttga
    10141 tttccatact cttcctccta aaattcgtta tagataaaag ttcctccttg aatattttta
    10201 tagtgataca gataaatcag gaacagcagt actgcactat aaaaaattgt tcgaatgatg
    10261 aattttcccc aataattcaa ttttggatta gaacgccatt tgttcattgg attccctcct
    10321 catataacct cacacaaagt gtaactaatt ttgacaaaaa gaaacatgtg tctagcttac
    10381 agaaaccgtt tttttactaa cagttttgtc acatagcttt tataaaaata gcacatcaaa
    10441 tcactgcttt tttctttata atttctcgta cttttaaaaa atataaaaaa taaagaggtt
    10501 gtgacaaaaa tcgtgtcaca acctcttatt ccaaataaat ggtattcaaa atttagttct
    10561 atttattatc ttgtcttatt ccgtatatca ttcctcgata atccgacaat ataactgact
    10621 cgtaatgaca taaagcaaaa gatagataag gatcaataag ccactcacaa gataagccaa
    10681 ccagtaatcg gcagttttta tatactgagt gaagacattg attgcaaata ctgcgtgagt
    10741 gatccctaat atcatgggtg gaaagaaggt aagtgcgttt tgctcgaaga tcatccgtct
    10801 gatccttctt tgtggaatcc ccagcttagt caataggtga taattttctt tttcttcctc
    10861 tgcttctgct aattgccgga ctgtcaaaat actaccagta gcaataatca cgatcatccc
    10921 gacaaatagt gtgacaaaaa tattgatacc gattcgccga ttaactgcac gcaatttcga
    10981 atagctggaa gagaaattca aacgagagct ttcttggcta aattgtgctt ccttcatttg
    11041 gtcatccgga agtttctttg tgctaatttc tccttgtaga cttccattta cgatatcata
    11101 agaataataa acagcattga gccactttat cgacaggtca ttgctcagtc gttcatctat
    11161 tttttcatta tttccacctg atacatctac attgactact tgataatcca caccttttgc
    11221 tttttcaaaa acggtatcat ctacaatcag tacaggactt agatatacca ttgtttcgtc
    11281 acctaataca cttggaagca tttgttgaat cttcaattct tgattatctg gcagtccaat
    11341 actagatcca tataatgaaa tattccgaaa aagagtttgc atttgatcaa gcaaaacagt
    11401 atgaccaggt gatttcaaag aaatgttcgg caaatctgga ttaacttttc tgaaggcttg
    11461 atattgtgtt tccgtgatca gattagcagt ttgaaacata ggttcttcga gattttgact
    11521 atagatactc ccaatgactt tataatgcaa ggtgatttct ttagttatct tctgcccttc
    11581 ctctgctaga atcggcttga tttttttctc tgtctcaaca tcaaactgat agtccatcgg
    11641 ggaggaaact ttctcctggt aggataaaat agtagagata ctagccgtgc cgccaattgt
    11701 cattaaagaa attccgagaa tgactgtaat cagagaattt gtacgccaac ttttgagcaa
    11761 atgaacttgc gtgttaccca ttatcaaaaa attgatacca tagtaataaa tcttccattt
    11821 actcaatatg atcaaaaata accgaaatgt ataacagaaa aacaaataag taccgaccac
    11881 acaaacgaaa aaaattaaaa aaggaaaaaa gaaaactaat gtatcaagat aatgataact
    11941 gatcatccat gaagtgattt ccctaaaatg actagctcca aaatatccgc tcgttaaaag
    12001 tagtaacccg atgactgcga gtaatcggtg ccttgtcctg aagcgcattg ttgaactatc
    12061 tgcttgttct ttttctccaa aacttcgaat cattggatag cgccatattt tccatattga
    12121 atatacagat actgctaaaa ggatgatgaa aaaagcaaat aaagtatcta ctactgatgg
    12181 aatggagata aaaaatggac tagtaagatc catatccatc attcgaacca aaatcattga
    12241 aaagagtttg gaaaaaataa taccgagtaa tatcccagaa atacaggcga acaatccaat
    12301 tatcatgatt tctaacacat atatacctga tatctgcagt ttattcatac caaaaagctg
    12361 atagatgcct atttcctttt gccttctatt caagaaaaat cggttggcac tgaatacgaa
    12421 aaaaagcaaa acaacagtaa tgataaatcc accaaaactc aatccaccat ctatcgaagt
    12481 atcttgactt gctcttctga ctaaagattg atcgtaggtc attgcgctga aggaataata
    12541 gatcattacg gataaagtca aactgaaaaa ataaacaaga tagcctcgtg actgccgaaa
    12601 aaaactttta acggctagtt tatacagcat tcatttttcc tcctccgcca atcgtcgcct
    12661 gcatgtctat tactttctca aagaactctc ttcttgtccc tcttctgaca acttcagaaa
    12721 aaatcacacc atctttaata aataaaattc tatcgcagta gcttgctgta tatggatcat
    12781 gggtgactaa cagtatcgtt gctttttctt tttgattcat ggtcttcaag tagttcaaca
    12841 attctgtagc cgacttcgag tccaaagagc cagttggttc atctgcaaaa atcacttgtg
    12901 gctgaacaac aagtgctcta gcagctgcaa ttcgttgtct ctgtccgaca gaaagttcat
    12961 cgggataccg tttgagcagt tcttctatat tcaacaattg agctacatgt cttactctct
    13021 tttccatttc ctctttagtc atccgttcaa ctgctagggg caataggata ttgtcttttg
    13081 catttagggt atcgatcaaa ttgaactctt gaaaaatgaa tcctagtttt tttcgacgaa
    13141 agtcactgag ctcactgttt ttcatttttg tgatttcttt tccttggatc gagacactgc
    13201 ccatcgtagg caaattgatc gtggacaaga tattcatcag cgttgtcttt cctgcaccac
    13261 ttgggcccat aatcccaaca aactcgcctt cttctactgt gaatgagata ttatctaagg
    13321 ctttgacggg agaaagtttg ctgccgtagc ttttacttag atgtttcact tcgatcatct
    13381 tttccaattc ttctcctcct ctttcatttt tcatttctat ttatttgcat agtagcatat
    13441 ctacgaataa tttgctctta tgtttttgtc acttttactt ggactgaata aaaaatttta
    13501 ttcaattatt ttattcagca aaattaaata tgcaaaaaac aaatccacca gctacaagtt
    13561 ttcacctttt ttcacaataa aatatacact acatatagta tagaaatcca attatacagc
    13621 acattgaata ctatatatag ccgatattta gttgctcctt ccccgatttt gttttatgat
    13681 agacaagaac aatttagcag gagttgaaga taatgaaagt aaattatgaa gaaaaaactg
    13741 tcgttactca gcagccagag acatcccaga tgaaagtcat caaacgcgat ggccgccatg
    13801 ttgatttcga tgaacaaaag atcaatgaag cactgatcaa agcagaaaag aaaattcacg
    13861 gctcattatc accacttact tatgaaaaaa ttcaaatgat tgcagacttg gttgtccaag
    13921 aaatcaaaag ccggtttgct gaaaatgtca aaatctatga gattcaaaac atcgtggaac
    13981 atatattact agaaaaaaac gaatatgaat tagcagaaga gtacatcaat tatcgtacca
    14041 aacgagattt tgctcgcagt aaagcaacag atatcaactt ttcgattgaa aagctaatca
    14101 acaaggacca aactgtcgtg aacgaaaacg caaataaaga cagtaatgtt ttcaatacac
    14161 agcgagatct gacagccggg atcgttggaa aatcaatcgg actgaaaatg ttgccaccac
    14221 atatagcaaa tgcgcatcaa aaaggcgata tccattacca tgatttggat tatcatcctt
    14281 atacaccgat gacgaattgc tgcttgattg atttcaaagg catgctgaac aatgggttca
    14341 aaatcggcaa tgccgaagtc gaatcgccaa aatctatcca gaccgccaca gcacaaatct
    14401 cacagattat tgccaatgta gcgtcaagtc aatatggtgg ctgttctgcc gaccgaacag
    14461 atgaattgct tgctcctttt gcagaattga actataaaaa acatctaaaa gacgcggaag
    14521 aatggatcga ttcgccagaa cgccaaaaag aatatgcaaa agcaaaaacc aaaaaagata
    14581 tcttcgatgc tatgcaaagt ttggaatatg aaatcaatac cttatttact tcaaatggac
    14641 aaactccttt cacttcatta ggatttggac taggagaaaa ctggtttgaa agagaaattc
    14701 aaaaagcaat tttacagatt cgaatcaatg gattagggag tgaaaagcgg acagctatct
    14761 tccctaaact gatcttcact ttgaaaaaag gtgtgaactt aaaccctgaa gatcctaatt
    14821 acgacatcaa acaactagca ttggaatgtg caacgaaaag aatgtatcct gatattctga
    14881 attacgataa aatcgttgaa ttaactggaa gtttcaaagt acctatgggc tgtcgttcat
    14941 tcttgcaagg gtggaaagac gaaaatggtc aagaagtaaa tgtcggacga atgaatttag
    15001 gtgttgttac attgaatttg ccaagaatcg cgatcgaatc aaaaggagat cagaataaat
    15061 tctggcagtt actgtctgac cgcttagaaa tcatgaaaga tgcgcttctc tatcgtgtag
    15121 aacgttgtaa agaagccatt ccagcaaatg cgccaatttt atatatgtat ggtgctttcg
    15181 ggaaacgtct ttcacgtacc gattctgtca atgaattatt caaaaaccgt cgagctactg
    15241 tatcgttagg ctacattgga ttgtatgaag ttgcttcagc tttcttcggt ggcgaatggg
    15301 aaacaaaccc agaagcaaaa gctttcacac ttgatatcgt caaagaatta aaagctaatg
    15361 cagatgcatg gggggatgaa tatggttatc atttcagtgt ctattcaact ccaagtgaaa
    15421 gtctaactga ccgtttctgc cgtttggata cagaaaaatt cggcatcatc gaaaatatca
    15481 cagataaaga atattataca aacagtttcc actatgatgt gcgtaaaaac ccaacgccat
    15541 ttgaaaaatt ggaatttgag aaggattatc caaaatattg ttcaggtgga ttcatccact
    15601 actgtgaata ccctatgctc caacaaaatc cgaaagcttt agaagctgta tgggattttg
    15661 catatgacaa aatcgggtat cttggtacga atacgccaat cgatcattgc tatcaatgtg
    15721 gtttcgaagg tgatttcaaa ccgacagaac gagggttcga atgtccagaa tgtggaaatc
    15781 atgatccaaa aacatgtgat gtagtcaaac gtacttgtgg ctatttagga aatccgcaag
    15841 ctcgtccgat ggtacatggc cgccataaag aaatttcctc acgtgtgaaa cacatgaaat
    15901 gaggttgtga aaaagctgtc ctgcatcaag taataagaat agctaaacaa aaatagttcc
    15961 tcatattttt cgtttagttg ggcttattac tgcagatgca agcttttgaa caccgtttat
    16021 tcagaataag aggctatgac aagtcttttt tcaccaccct ctttcgatgg ctttgtatct
    16081 ttgaacgccc tctatcaaaa taagaacaag ctttcttgaa caatggattt aggtcagttt
    16141 gttcttattt atgtggtcga gtagatttta tcttcaatag ctatatcttt gaaccttaaa
    16201 tgaaagaagt ttttttatga gaaatccaaa accaaaagaa tggttagcaa aagactacag
    16261 ccaaaactat attgctgact acaaaccgtt taattttgtc gatggtgaag gtgtccgcaa
    16321 cagtctttac gttagtggct gcttatttgc ctgcgaaggc tgcttcaaca aagctgcaca
    16381 aaattttcga tatggcaaac cattcacacc tgaattagaa gaacaaatca tcgctgactt
    16441 gcgcaacgat tacgtgcaag gattaacttt acttggtggc gaaccttttt tgaatacaga
    16501 tgtttgtctg caagtcgtac gacgtatccg aaaagagttc ggtacgacaa aagatatctg
    16561 gtcatggaca ggctatacct ttgatgaatt gctacaagat tcagaagata aattagaact
    16621 cctttcgcaa atcgatatcc ttgttgacgg acgctttgaa ttaagcaaac gtgacctaaa
    16681 actacaattt cgcggaagta gcaaccagcg gatcattgac gtccaaaaat ctttagaatc
    16741 caatcaagtt gtgatttggg aaaaatgtac ggatgcaaca gaaacctatg aacaaatcaa
    16801 aaagcaagac ttgatataaa aacagctttg ggagagaaaa cggatttccg gtttcaactc
    16861 tcaaagctgt ttttatattt cttgattttc ccataatgga aggacgatgt tttcataagt
    16921 catcgggtcc agattcgtca aagtaatccc taacaatcgg attccttttt ctactcccaa
    16981 gatttcctcc catataaggc ttgcttgata aaagagtgct tcttttttat agatatattc
    17041 tggcaatgtc actcttttgg tcacagtaga atagtccgtg tacctgacct tcaaaactac
    17101 tgttttccca tgtttttgga ctcttctcag tgcttcttct actttttcag ctagttggcg
    17161 caactgagtc agcactgctt cttccgtttg caacggctgc ccataagtat gctcttttcc
    17221 cacagatttt cgctctcttg taacgtttac tggagaatca tggattcctc tgactttacg
    17281 ataaagagaa tagcccattt ttccaaaatt tcgaatcaac atcatttctg tgcattcata
    17341 taggtctttt cctgtataaa tccctagctc atgcattcta ggtacagttt tcttccctac
    17401 cccatgaaat ttttcaattg gcaaagcttt taaaaatggc acagcgtctt gaggggcaac
    17461 taccgtgatt cctctaggtt tttgatagtc tgatgctaat ttggccaaaa acttattgta
    17521 acttactccc gcagaacatg ttagctggac actctcccaa atatctgctt ggatcattcg
    17581 ggcgattttg atggctgact tgcaattgat tttattttcg gtcacatcaa gatacgcttc
    17641 atcgatacta actggttcca caatatctgt ataacgatag aatacttccc gtacttgttt
    17701 ggaaatatcg ctgtatttat gatggtcgcc aggcttaaag atggcttgcg gacaaagctc
    17761 atacgctttt tgtgcactca ttgctgaatg aattccgaat tttcttgctt cataatttgc
    17821 tgtagtcact actccacgcc caccagtgtc actggggtgt ctggcaatta ctaatggatg
    17881 tccgcgaaac tcaggatgat ctcgttcttc aaccgaagcg aaaaaagcat ccatatcaat
    17941 atgaatgatt tttcttgatg tatcattatg taaaggaaat tgcagctcca tcatcctcac
    18001 ctcttattca ttatacaaga acattcgttc gcgttcaaga gatttgaaaa gttagtagct
    18061 gatttcctca ttagtctttc attatagaca acttatacta tctcagtcta attcatggta
    18121 ctgacggtag acttcttgtt ttttcagccc atttttcagc gctactgctt tgatagcagc
    18181 atttggtttt tcgcctgaag cgatctttgc ttctacttgt tcctttaacg ttcccaactc
    18241 ttcatcaact tccgtcactg gcatttcgaa caaattccct tctacaagca gacaacattc
    18301 accctttact ggatgttctt cgatataagc gagtagctct tccgtacttc ctctaaggta
    18361 ttcttcatgg acctttgtca attcccgaca caaaacgact tggcgatttc caaaaatttc
    18421 taagaaattc gtcaatgtag cagtgatccg ataaggtgac tcataaaaaa tcagcgtaac
    18481 tgtctgttct tttagctttt tcaatgtttc tttctgttct tttttcttac gtggaaggaa
    18541 gccataaaat aaaaatggtt gaggcactag ccctgaagca atcaaagccg tcatcccagc
    18601 tgtaggtcct gggatagaga tcacagaaat cccttctttt atgcaggcta aaactagctc
    18661 atgtccagga tcgctgatgg aaggcattcc tgcgtcactg acttgagcaa tcgtttttcc
    18721 ttcttttaac aaagcaatca attgagggac acgttcttta taattatgtt catgtaaact
    18781 tttttggggt gtatggattt caaaatgatt caataacttt tgtgtattac gcgtatcctc
    18841 acttgcgata agatcggatt cttgcagcat acgaacactt ctgtagctca tatcttctaa
    18901 atttccaatt ggcgtcggta caagatataa atccccatat accggatttc ccttgaaact
    18961 tttttgatta tacatctgtc acaatctcct taataaaaag actggagcag tatttctccg
    19021 ctccagtctc ctcttgttat tggtcatcga ccgctttcgc gatagatcac ttctagacaa
    19081 aacgcacaag gctcgtcatt gtcccttctt gatccatata aatcgtagca gacatggaat
    19141 ccttcttcat aaattttttc aagattcata cgagacttag aaagttcctg cttcacattt
    19201 tcagctggag cttgtgcaac ttggttcaat tcccgcaggt gttcccgcag acgttgattt
    19261 tccatctcca aagttgtatt tttttctacg atctcatgca aagcatgctt catttcaaca
    19321 agttctgtta atgtatttct caactctgat tctaattgat tcaatccgtc gtataatgat
    19381 ctcttatcca ttcatctcac ctacttggat aaattctttc aattcatccc attcatattc
    19441 gacaggattt tcccgtccag tcagccgaat actaacgata cggctaagca gattcaaccc
    19501 tacgactttc cctttaccat caggtgtagc aattactttt ccaaagtcag ggagttcttt
    19561 tttcgctgtc tcgtattctt cgctctcata ttgcagacag cacatcaagc gtccgcataa
    19621 accggaaatt tttgttggat tcaaagataa actttgatct tttgccattt tgattgatac
    19681 aggcacgaaa tcgcctaaaa aagaagaaca gcataatgga cgcccacatg gaccaattcc
    19741 gcctattatc tttgcttcat cgcggacacc aatttgtcga agctcaatac gagtacgaaa
    19801 tacagcggcc agatctttta ccaattctct aaagtcaatt cgcccgtccg ctgtgaaaga
    19861 aaaaatcaat ttatttcgat caaatgtgta ttcggctttg accattttca tttccaactc
    19921 acgcaactgc actttttctt ttgcgatttt caatgcatca taagcatctt ttttgttttt
    19981 tttgtctttt tctaattcag cttgtgacgc tttatgcaag atcggacgga tagcttcagg
    20041 aaggtcattg ggatcaatct cttttttagg aaggacaaca gtagcaatat gtaaggattg
    20101 ctgagattcg actaatacct tgtcactata aacatattca gagtctcctg gtgcaaaata
    20161 atagatatga cctgcatctc tatatcggac acctactact tctaccattt ccatcctcct
    20221 tgatcatcat cctttgcggc aagctaaaaa atcgtatgca aaacgaactg ttccgcaaca
    20281 gcctgaaaac tgacattgct gcgtagtttc tgctctgcga tcaatatccg ttcaattatc
    20341 tggtttatct gtttcacttt gccatattcg ccgtttgcta ctgcttgacg ctgtgcttcc
    20401 tgataataaa acatcaagac agataatcct gtaaactgtt gtgttttttc tttaaatgat
    20461 tttaccaatt ttttttggac atagataaag gcttgagtat cttgcttttg caagtaaacg
    20521 aaccattgat aaatcaagtc cttagcatca ttaaaccatt catcttgaga gatttcaact
    20581 gcttttccta aactgtttgt taagaaagcc aaaagttttg ctttttccaa tggaatctgc
    20641 tcttcttgca atcgatggat cagtgcttct ttcgaaagtt cttgaaaatg cagaacttga
    20701 caacgagatt gaatagtagg gagaattctt cctactgcat ctgtttctaa caccgctaaa
    20761 aaatctccag ggggctcttc caagaatttc aacaaactgt ttgccgcact gctattcatt
    20821 ttttcagctt ctttgatcaa aaaaactttt tttcttgatt cgtaaccgct gcgactgaat
    20881 tccgtctgca aacgtctgat ctgatcgact ttgatggttt gtccttccgg ttcaataacc
    20941 aaaacatctg gatgttcttt cttttcgatc cgctgacaat tgttgcattt tccacaaggc
    21001 agattgtctt tcatttgggt acagaacagg tgctgtgcaa gccatatacc tacttcatgt
    21061 tttcctgttc ctttctcccc ttcaaaaaga taagcatgag cgatacgccc atgctcaaaa
    21121 ctccgttgaa gctgctgata cacgatcggt tgcatttgag ccaagtttat ttcttgattc
    21181 atagctcagc tcctcctaaa attgatggaa tccttcaaca ggtaagacga agactgtggc
    21241 accgcctact tctacttcta ctggataagg gacgccccca tccatcgaaa tatccagtgt
    21301 aacaggtgta gatacaaatt gctttcttga ttcacacgtt tttttgatga tttccaatgc
    21361 ttcttctaca cggtcatctt caatacccac gataaacgta ctgtttccag ccttaaggaa
    21421 gccgcctgtc gatgatagtt ttgtggcacg gatatttgca tcaatcaatt catttgataa
    21481 acggttgctg tctttgtctt ggataatcgc taaaataatt ttcataaacg ttcaccttcc
    21541 tagttttcaa aaaattgcgg atactgttca ataattgtat gataacttgt ttgcagcact
    21601 tcttcaaagc tttttcttgc atcaactttg tggattcttt ccggattctc ttctgccagc
    21661 tttaagtaag catgacgtac acgctggtgg aattccaaac cttccgaatc taaacggtcg
    21721 atttgatttt gtctgttttt cttgattctt cttaatccgg tgtctgaatc tacgtcaaga
    21781 taaagggtaa aatccggcgt agtaccttct gtagcaaact cattcaaccg agcaatctcg
    21841 gtaacgccga tgcgtcggcc tgcaccttga taagcaagag aactgtcgac aaatctatcg
    21901 cataaaacga ttttcccttt tgccagagca ggcagaacct tttcgactag atgttgtctt
    21961 cttgctgctg cataaagcag tgcttccgta cgttcatcca tccgatcatt ttttggatca
    22021 aggatcactg ctcggatctc ttcagcaatc ggaatgcctc ctggttctct tgtttgaatg
    22081 atttctgttt ttgctacttc tttcaaaaga gggtaaagtt cattcaagat gctggttttt
    22141 cctgcaccat caggcccctc gattgtaata aatagtccgt tcacattgtt gcccccatct
    22201 cttgtgctgc ttttatattg tatctgaaaa aacacttaat tagaaattta taactcacgt
    22261 cttccttcga cagctttcaa taaagtaact tcatctgcat actcgatatc agagcctaca
    22321 gacagaccat gtgctagacg agtcactttg atacctgcgg gtttgatcag acgtgagaga
    22381 tacatagctg ttgcttctcc ctctgttgtt gcatttgttg cgataatcac ttcttttaca
    22441 tctgcatcat gcagccgctg caacagagaa gaaatattga tgtcttctgg acctgtccct
    22501 tccatcggtg ataacacacc atggagaaca tgataaagtc cgtgatattc tcgcattttt
    22561 tccattgcca ttacatcttt cggctcttcg accactagga taacgcttcg atcacgtgaa
    22621 ggatttctac atatttcgca gggatcttct tctgtgatgt ttccgcagat actacaaaaa
    22681 tgcaaatcgc gttttacact taacaaagat ttagcaaatt catttactac ttcatctttc
    22741 atatcgatcg tataaaaggc gagtcttgtc gctgttttct gaccgattcc tggaagtttc
    22801 atgtagctgt cgatcagttt cgcaattggt tctggatatt gcatattcat tactccttca
    22861 gtttaaaact aaaatcctgg caaacctttt gcgtatttcc ctaatttttt ctcagtttcc
    22921 acatcaattt gtgcgatcgc atcattgact gccattacga caagatcctg aagcatctcg
    22981 atatcatcag gatcaacgac ttcttcattg atttggatat ctttcatgcg acgatcgcct
    23041 gtaaaaaccg ctttgaccaa ttcattggtg gctttacctg taaactctgt tttatttagc
    23101 agctcttgtg cttctgccat ttctttttgc attttttgta cttgtttcat catgccttgc
    23161 atattgccca ttccacgcat cataattatc tactcctttt tattaatcat ctaccactgt
    23221 aacagcttcg gagccaaaca gttcctctgc ttttactaca agtgtttctt ctggtacttc
    23281 ttgttcttga ggaagcaact cgatttctcc ttctgagtct tcagaagcaa aagagttttc
    23341 tgttccctct tcttgtgcct gcgtcaaata atttcttcga agttctggcc agctttctct
    23401 tgtaatataa acaggatcag gcgcataatc tttgatcatt cggcttaagt tgttgtgtac
    23461 cgaaagctgc aattcttcgt catttgctgc tctttgacaa acaatctcat aatcaaacgc
    23521 aatcactagt cctgtaggtc cagctgcaac tggttcgctt gctttaagca ttgctcgctg
    23581 agtgacagat agactcatca gcaagtcttc ccatacttct tttacttggt tcagatgctg
    23641 tcttgtcgct tcccgcagaa ctttatatac tcgttccgtc ggtacacgat aggtacttgt
    23701 ctgtttttta tgatttgttt gctgtaccgg ctgtactttt gtattctcaa ttcctgtatt
    23761 actgcgtagt tccttgattt cgtttcttag ttccttaatc tcttgttgta gctgttttac
    23821 tatctgatca tctgcaaatg ctacatctgc tgaaggaaca gaagcagacg gtagctgtgc
    23881 aagtttgact gtagcgactt ccaaataaat agcggcacta ttggtaaaac gaatttcatt
    23941 ttgagtatcg ctcaaaatct ttatcattga aaacagctga tccgctgaaa cagtttgtgc
    24001 caattctgta aatgtacttc tgatcgtact cgattgttcc tcaactaggt taggtgcttg
    24061 ctgatacatc agcaagtcac ggcagtattg taacatatcc tctagaaagc gacgtgattc
    24121 tttgccggct gcgagaattt ggttcaatgt ttccaatgct tctggtactt cttttttcac
    24181 gcaagcactc aagtaactgt ccatcatttc gtcagttaag ctacctgtca cttgcatggc
    24241 atcatctaaa gtaattgttc cttcactaaa cgagattgcc tgatcaagaa tactcaatgc
    24301 atcccgcatc ccgccttctg cagaacgggc aatcacattc aatgcagctg gttcatagct
    24361 gatagtcgat tctttcaaga taaattcaag atgttcgaca atatctgccg tattgatccg
    24421 tttaaaatca aatcgctgag tccgtgaaat gatcgtagct ggtattttgt gaggttcggt
    24481 cgttgctaag atgaatatga cactttcttt tggctcttcc aatgttttca acaaggcatt
    24541 gaaagctcct gttgaaagca tatgtacctc atcaataatg tacactttgt acgctgcctt
    24601 tgtaggcgca taatttgccc gatcacggat aaatcggatt tcttcgactc cgttattgct
    24661 ggcagcatcg atttcgatca catcttcttg cgtaccagca gtaattgatc gacacatttc
    24721 gcattcgttg catggctcac cgtctttact gttcggacag ttgattgctt ttgcgaaaat
    24781 ctttgctgca ctcgtctttc cggttcctct gggtcctgta aacaagtagg catgtgaggt
    24841 tttgtgggaa acaatagcat tttttagtgt ctgtgtcaca gctttttgtc caacgatgtc
    24901 ttcgaaacgc tgcgaacgcc agactcgata aagtgcttga tatgccataa ggatccccct
    24961 tttttaaaaa attcactatc tcttattata cgtgatatta ggtaaaaaaa ctactatcct
    25021 tctcggcttt ttcaggtatt taaacaatac gcaattttcc atttcaattc cgtctctagt
    25081 ccgagaagtt tcacatcttc ctatggtata ataaataaac aataattgtt tggcactagc
    25141 tgaatcagag gaggaatttt ttatgggtct tatcaaagca gcaacaagta tggttggcgg
    25201 cggtctggca gatcaatggc tagaagtcat cgaaccagac aatatgagtg atacaacggt
    25261 catgacaaaa ggtgtcaaag tccgcaaaga tgacaaacgt ggctccaatc gtaaaggaac
    25321 agaagatgtt ttaacagacg gaacagtcgt ccatgtctat ccaaacatga tgatgctttt
    25381 ggtcgatggt ggaaaaatca tcgattatac cgctgaggaa ggttattaca ccattaaaaa
    25441 tgacgcagct ccttccatgt tcaatggaac actgaaagaa gcaattgctg aaacttttga
    25501 ccgattcaaa tttggcggcg tcacaccgca gaaacaacaa gttttttata tcaatctcca
    25561 agaaataaag ggaatcaaat tcggtacaag cgcgccgttg aactactttg acaatttcta
    25621 taacgcagaa ttattcttac gggcacatgg cacttattct attcatgtcg ttgacccgat
    25681 tttattttat acaaacgcaa tccctaaaaa caaaacacaa gtggaaatca atgatatcaa
    25741 tgagcagtat ttagctgaat tcctaacagc gctacaatca gcaatcaatc agatgtctgc
    25801 agatggtcaa cggatttctt atgtaccatc taagagtttg gaattaagca aatacatgga
    25861 tactgcattg gatgattctt ggcgtgaatt acgtgggatg gagattgttt ctgtggctgt
    25921 agccagcatt tcttacaccg atgattctgt gaaactgatc aatatgcgta acgaaggggc
    25981 gatgctaggt gatcctagcg tacgtgaagg ctacgtgcaa ggttccattg cacgtggtat
    26041 ggaagcagcc ggaaaaaatg aagcaggagc aatgaccggc tttatgggtg taggtatggg
    26101 aatgaatgca aatggttctt atctttctca agcagctcaa aataaccaag aacaaatcaa
    26161 acaacaagca gaaaaacaaa atcaacaaac tgcacaagga aatgcggata cttggacatg
    26221 tccagtttgc gggacagaaa attccggaaa attctgttcc aactgcggag ctgccaaacc
    26281 tgtagaaaac gttcagccta agctacaaat gcgatgcagt gaatgcaatg agatcgtgga
    26341 tctttccaac ggcatcccta aattctgtcc aaactgtggt aaaccgttta aaggaatccc
    26401 cgttaattaa tttatttaga aaggtgtgca gctgatggat gttcttacac ataaatgtcc
    26461 taattgcggc ggtccgttga cttttgatcc caaagaccaa aaatttcatt gcgaatactg
    26521 cctgaatatc tatacagaag aagaagtcag ccaatatgag caaaagcaaa aagaagcgcg
    26581 tatggctggc gaagaaagca aagaagaacc ttcacctacc gaagatttta cttttacagc
    26641 ccaagaacag cttgccgata tgaatgaagc cgagcgcaaa gcggttgacg aggcaggcgg
    26701 tttttcagat acagcggacg aatctgcaga taataccgca gcgatagatc tttttctttg
    26761 tcccaactgt ggtgcagaga tcgtgactga tgcgacaact gctgcgactt attgttacta
    26821 ctgccataat cccgtcgtac tatcgggacg tttaagcgga gaattcttgc caaataaagt
    26881 acttcctttt gctgtcgaga aagaagaagc aatcaacaga tttctcgctt ggaccaagaa
    26941 aaaatggttt gttcccaaag cttttttcaa taaggatcaa atcgataagt tgacaggtgt
    27001 gtatttccct tattgggcta cagatgctga agtagatggt gccatgcagg cgaatggtac
    27061 agttatccgc atatggcgga taggtgacat cgaatacaca gaaacgaagc aatttgctgt
    27121 ttcaagagca ggcaagcttt cttttaaaga actagtaaaa aatgccttat caaaaaatac
    27181 gcaacaaaag atggtcgaag gcgtgcaacc tttcccgtta gataaagcaa ttgattttaa
    27241 aagccagtat ttagccgggt tccaagcaga aaaaagagat atcgaatatg aggcaatcaa
    27301 atcacagatt caatctgaat taaaagacta ttcagagaag ctgttaaaag ataccgcaaa
    27361 tggatataca acattgaccg gtgtgcgaac ttctgcttca atcacaaacg aagtaaataa
    27421 ttacgtatta ctgccggtat ggcttgttac ttatcgaagc aatgactcaa gtaaaaaagt
    27481 ctactactac gcaatgaatg gacaaacagg aaaggtcagc ggtgtcctgc cgatcagcca
    27541 gaaaaaattg ggactgaccg ctcttgggat tttcgctggg ctagcgatcc tctttctgat
    27601 cggaggttac ttgatatgat gaaaaaattt tttttattct ttcttttgct agtaagtgtt
    27661 ggctggccgg tggctactca tgcagataca gctacggtaa atgatgaggc tgggttattc
    27721 acaacagatc aaatccaatc tttggaacaa caggcagaaa agctgaatga aaaaatcaaa
    27781 ggacgagtct tgatcgtaac gactacatct aattcagaag aaccaagaga ctttgctgat
    27841 aattacttaa gagacgcagt cggaaatgat caaaatggct cagtattgct attagatatg
    27901 ggacagcgtg aaatctacat ttcgacttct ggtaatatga tcgattattt gactgatagc
    27961 agaatcgatt cgatcttaga tgatgtttat gatcagatga caaatgctgc ctattacaat
    28021 gctgcaaaag cttacctgtc taaatcagca acatatgtgg aagacggagt tccaggagga
    28081 cattatcgtg tagacgaaga aactggcaaa attacacgct ataaggtttt gacgacagta
    28141 gaaatcatca ttgctgttgt tttggctctt atccttagtc ttgtctttta ttttgtgact
    28201 gtttcgcgtt atcaattgaa atcaggcatc tacaaatatc cattccgaga aaaatccagt
    28261 atcaaattaa ctgaaaaaac cgatcgtctg acaaattctt ttgtcacgac aagaagaatc
    28321 cccaaaagcc cacctcctgg aagcggcggt ggcggaagta ctacccattc tagcggtggc
    28381 gggacttttg gcggtggcgg cagaagtttc taaaaattgc taaatgaatg ttcgcaaaaa
    28441 aagggctgtg acaaaaatcg tgtcacagcc ccttttgttg caaataaacg gcgttcaaaa
    28501 gcttacatct acggtgataa gtccagccaa gttaaaaaca taagagactg tttttacttg
    28561 gctcttctta ttacttgatg cagaacagct ttttcacaac ctcttggtaa acggtgttct
    28621 cctaccaact cctcggtcac aagtcacaat attcatcaaa catgaaaagc tgttttttga
    28681 aattgttcct tgtgactcag agttaaacgg caggctcaca acctcttggt aaacggtgtt
    28741 caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc
    28801 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttg ttcatcaaaa
    28861 gataatattg tcttctaaca agtccatatt caaaataacg atatgatggt ctttaacggt
    28921 gataatacca tcttttttca gttgttttaa aattctgttt acactgctag cagttgagat
    28981 gccacaaaat ttggcgattt cttcattcgt gatcgaaaaa tcgatcagca tcccttcttc
    29041 tgtccgcaca ccgaataaat catataattc gtaaagttgc gtacaaacag ctcccagctt
    29101 tccgtttaat agcatttgct gcattttttt catcgaataa agcagacgat agcggtaata
    29161 ctcttttaca tagtgttgta gctttgggtc ttggttgata ttttgccaaa agcgcacacg
    29221 attgattcga taaaactctg catattctga ttcaactcga atattatacg gcgaatcgat
    29281 gaattgtgaa tattcgtctt ttaaaagaga gacgatttca agatctttga tatagcgaag
    29341 gttaaattct ctcccatcat ttgaaataac acttgctttt accgttccct ctttcaagat
    29401 atacgtatgg gaatcttcca tcccttcgaa tgtcaaatat ttatgatatc ctttgcgtat
    29461 aatcggaaaa tcatttttat ccaaatattc ctttaagact ctactatcca atgatgctcc
    29521 cccatccaaa ctttcatcta aaaagatcat acagtacttt aaaacttttt aaagtccaaa
    29581 ggtattttac taatgatatt aacatttgtt aagtaccttt ttccatcact tatgcgattt
    29641 ttttcctttt ttttattcgt tatactgcag aagtaaaatt ttttaagaat agagggaaaa
    29701 aacatgaatt actggatgaa cacaatcatt aatcgattag aaacagcata tcaaacaaga
    29761 tttgatatga aagcttcttt agtcttttta aatgatgcgt atcaaaactc gatcgaattg
    29821 atcaaagcag tggatgagaa tccaacaaac gaatgcgaag aatttttaaa tctgtttatg
    29881 tctacaagag atttatttat tcgccaactt gttgatcgct atccatccaa ttatcacgat
    29941 gtagaagttc aaatacaaaa attaaaagca tattcagctt aaggggaatg tataacatac
    30001 atggaaaaga aattatttaa taaatatttt attggaatca cattgatcaa ttttattgtc
    30061 tacctgattt attatctgtt gatggtcatc atggcagtca ttgcgcaaga caccttacac
    30121 gccagcttga gccaagcagg ttttgcttct gggatctata tcatcggaac attgctggca
    30181 cgcttattca tggggaaaca gttagaacta tttggtcgga agtttacctt aagaggcggc
    30241 gcgatcttct atctcctttc aacattagca tatctttata cgccaactat cggggcattg
    30301 tatttagttc gattcttgaa tggattcggc tacggtacag tttctacagc tacaaatgct
    30361 atcgttactg catatatccc tgcatctaaa aaaggggaag gcatcaatta ttatggatta
    30421 agtactagtt tagctgccgc tgttgggcca tttttaggaa tgattttgct gaatatgact
    30481 gattttcgtt tcattatctg gttttcaatt gtactggtct tctttgttac gattgcctgc
    30541 cttgtctttc ctgtaaaaaa tattccatta tctgaagaac aaaaaatatc tcttcgctca
    30601 tggaatatca atagttttat tgaaaagaaa gcacttttca ttacaggaat tgcctttttg
    30661 atgggtcttt cttattcaag tgttttatca tttttatctt cttatacaaa agtaatccac
    30721 ttagttgctg ctgggtcatt tttcttcgtc gtatatgctt tggtcatcac atttacacgt
    30781 ccattgactg gaaggatttt cgatgtcaaa ggagaacaat acgtgatgta tcctagctat
    30841 ttattcttaa cagcaggatt atttttactg agtgtaacga cgaatagtgt gacattgctt
    30901 atctctggag cattagtcgg cctaggttat ggtacgttta tgtcaaatgg acaggctgtt
    30961 tgtttaaaaa tcgtccgcga acatcgaatc agtattgctt tatctaccta ctttattggt
    31021 ttagacctag gcttaggcgt tggtccttat ttgatgggga tgctaaaatc tcttacttca
    31081 tttagaggat tgtatgtgat tgccggagtc attccattga tatgtacagc actctatctt
    31141 gttaatggcc gaaaacatag tcatgaagca gagggcgaat tgattgaatc attgaaaaat
    31201 gaaatttgat cttgatgaat agataaaaaa aaccgttcag atctgaaaat aatctgagcg
    31261 gttttttgag taaaaatagt ttatgattat tcttaattaa acatctattt atttttaaag
    31321 ctttcattta tcgataagaa tagtttaact gttgatttat tccattttcc gttcatctgg
    31381 tacgatgcct tgacttagca accacttgtg gaagaaccat tctgccacac cgatcaatag
    31441 agcaggaatc aatactttca tccaaatatc gactgtatca ttcataaaat atagatagag
    31501 ccacaagata actgcagctg aaccagcatc tgcgatcgtc gctgcgatgt tccctacttt
    31561 acgtaagatc aacagatccc ctagcacata gatcatgact acaataatta ccccaacaat
    31621 cgttgaatcc ataaagctaa caccgtataa acctgtcagc acgacccaca gaatcgccca
    31681 gatcatgatt gccttcacta ccaacgcctt cacatgtttc attttctgca ctcctttctc
    31741 ctggataaaa aagtaagatt atctaacaag ataacctttt cattattata ccatcggctg
    31801 agagaagcga gcaaagataa ggtcctatct aaaaatgagg aagccactta gaacattcac
    31861 gtaataattt atccgtacag acattcgata tggtgttcct ttactttcgc atactgttct
    31921 tttgtcagtt ctttacttgt caccagataa tcgatttcat gaaaagcaat ggcttgatgc
    31981 atgaatttat gtgcaatttt ttgctgtgta actaaaacgg caagccggtc tgattgtttt
    32041 ctaaaagctt ttttgaccat tgcctctcct tcatgagttt ctgttactcc ttggctgaaa
    32101 tctagcccgc gacaactcat tatcgctaaa tccgcataat atgacaatgc atcgttatag
    32161 gcttttgaac cgttgattgt tgctcgtttc ggaacaattg cccctcccaa tacagatact
    32221 tttgccgttg tatattcact caacatagca gctgtgacga tgccattcgt aatgatctgc
    32281 aaaccatcaa atcttgctag gtttctgact aagtgtaaag aggttgtact ggaatccaag
    32341 aataagacca tatcatctcg aatcaaagaa gaagctgtat ctgcaatctt acgtttttct
    32401 gtttcttctt ctaatttggt gatcttcatc aggtaaggca cgtcaatttt tcgattgtca
    32461 ataagcatgg cgccaccgcg aactctacgg atcaatccac ttttttccag aaaaacaagg
    32521 tcacgtttaa tcgtggcttc gctataattt actaactcga tcaaatcggt gactcttgca
    32581 aattggttgt tttttagata attcacaatc tcctgctgtc tattctccac ttcattcaac
    32641 ataaaatcac cccatttact ttatatagta acaggaaaaa agaggttgtg acagaagtga
    32701 ttggcttcga gaaataagaa gaaatttttc gaaaaaacta cttctgaaca tcgtgtgtgg
    32761 ttaagagggt atgacaagac tattgtcata ccctcttgac cacacatttt tatttttagt
    32821 ataaactagc cttgcctact gtgttcaaaa tttccatttt atgtttgacc acttcttgta
    32881 cctttttatt agctgctgga taaattgcat tcggctcgta catcgatgga ttttcactca
    32941 atatttctct caaagcattg aaaaagacgc ttttaagatc tgagctcaaa ttcactttgg
    33001 caactccgta ttttactgct tcgctgactt ctgcgtccgg attacctgaa ccgccatgta
    33061 aaacaaatgg aagatcgatt tgttggttca attctttcag taagtcgata tttaattttg
    33121 gagttctgtt tttcggatac aagccatgcg ctgttccaat tgctacagca agtgtatcga
    33181 ttcctgtttc tttcacaaaa atcttcgctt gatcaggatc tgtataaatg attgtttcag
    33241 cacctccttc agcagaacca ttattcccaa tcgttccaag ctcagcctcc actgagacat
    33301 ttaccgcatg agctaacgca accacttttt tcgtaatctg tacattgtcg tcgaatgatt
    33361 tcaaagacgc atcgatcatc acggaagaat agccgtttcg aatcgcccgt aagacatctt
    33421 ctatcgtacc gccatgatcc aagtggataa cgatcggcag tttacttgta taagcatagg
    33481 ctcttactgc cgcaacaaac tcatcactta aataggcgat ttcatcagga tgaatttcta
    33541 agattactgg tgcttctaat ctctctgctt cttcgatgat tgctttcatc atatcatagc
    33601 tgcaaatatt gaaggctggt actgcaaatt gattctcttt agccactttc aataactctt
    33661 tcattgtata taacattacg cttccacctc atttgttaga ttttcatttt tcgtccactt
    33721 gtcttgtttt ttctctttca ctgttttttt caatagtcca agcataaagg ctgtagtgac
    33781 tgtacctgat gcaattgcca agacatacat aggtaaattg ttcataacat ttggaattag
    33841 catcacccaa atccctccat gaggtgccat tgaagtaact cctactgcca tggaaatccc
    33901 tgcagcgact gctgaacctg tcatcaaact aggtatcacg cggatcgggt cagataccgc
    33961 aaacggtata gcaccttctg taatatacga agcgcctaaa atccagcatg attgacctga
    34021 agtccgttct tctttggtaa atttatgttt gaataataaa gtggctaaag caatacctag
    34081 gggaggtgtc attcctgcag ccatacaagc agcaatcggt gcatttactc cagctgacat
    34141 caaaccgatc gagaaagtcg aagtcgattt attgatcggc ccacccatat cagctgccat
    34201 catgatcccg ataagtaatc cgaacaatag cccggatgta tttcccaagc tattcagcca
    34261 atttgtcaaa gcatccaaca cccatgtgat cggttgttca atcacaaata ccatcaaaag
    34321 cccaataatt aagatcgata aaaaggggac gacaatcagc tgataaatag cagaaaattc
    34381 tttttttacg ctaattttat ttgccagaaa atcacagata taccctgcta gcacgccacc
    34441 aatcattccg ccaagaaaac ctgaaccacc tgtagaggcc agtaatccgg caacaattcc
    34501 tggggcaaaa cctgcttttc cagcaatcga tgtagcaatc cccgcgccta acgcaggaac
    34561 catcatggca aacgctgtat ctcctccgat ccttgaaaat gcagctgcta acacattata
    34621 gtctgctgag ttgggatcag ccgctgtaat cccaaaggca aaactcagtg cgatgatgat
    34681 tccgcctgcg atcacaaatg gaagcatgta attcacacca ttcatgaaat gattatagat
    34741 tgtcggcttt ttctcatttg cagctggttc tttttcatta gacacaacga atacgccaat
    34801 atctctcaat gcttcgtcta ttactacttg cggttctttg attgctcggc ttgtagagac
    34861 acgctttact tttttcccgg caaatcggtc caattcgatt tctttatcta ctgcaagaat
    34921 gattgcatct gcctctttga tttcttgttc agataactga ttttctgttt ttacaccttg
    34981 tgtttcgaat tttgcttgat ggtttagatt tcttgctgtt acttctaatt tttctgccga
    35041 catataagta tgagcaattc cagtcgggca agcagttaca ccaacgattt tcatgattat
    35101 tttcctcctt tttcttcact atcgatactc ggacaaagac tctactattt tcattcttga
    35161 ctaatctgtc tgatgatatc tgctggcgta gtggcttctt ttattccctt tctaaaatct
    35221 gcattcatca atcgtctgga gagtgtgctg agaatctcta gatgcatatc tgctgatttt
    35281 tcaggaacta cgatggcaaa aatatattca attggcattt catctatccc gctatagtcc
    35341 gttaatttgg ctgtatgaaa aaaacacagt gctggttttg tcacatattt gctttttgca
    35401 tggggaatcc caaatccatc ttctattcct gtggagatct cctcctctcg tttgaatagt
    35461 gccttaacga ctttttgaac atttcttacc ttatttgttt ttttcaacac atcccctatt
    35521 cgctcaaata atgatccttt tgaatctatc gatggatcga aaacaatcag ttcttctgcg
    35581 atcaattgat caaacctcat aaaaaacctc tcccccacaa aaaatgttaa cgttttcttg
    35641 gaactgaggt cagtatagca tcgctagctg taccgttcaa tattatgaat gaacagagga
    35701 aaaagaccca tcatccgcgt gtataagtca ttaaaatgaa cgatctagct cacaaacaat
    35761 cgcaagtcta taacaaaaca aaaagccttc cttgaaagga aagcttagct tctaatttta
    35821 ggtaaatggt tttttacttc gaaataaccc ctcttcattt cgttaatata aggtgttttt
    35881 atcaagtgga taattaaaac tatatactag tttttcattg ttttgtgcat tatgatccta
    35941 cgactttttc agcatcaaac gaagttttaa attggcaaaa aacataaaaa aacagactat
    36001 ccctatcaga tagtctgcta aacgttgagt tatcagcgtt tttattattc cgcagcgttt
    36061 gcgtctttga gaccgtattt tttgttgaaa cggtccacac gtccgtctgc ttgtgtgaat
    36121 ttttgacgtc cagtgtagaa tggatgtgag tcagaagtta cttcgacacg gatgactggg
    36181 tatgtattac cgtcttccca ttcaactgtt tcttgtgaag tttttgttga acctgacaag
    36241 aatttgaaac cagttgttga atccatgaat actactgggt ggtaatctgg atggatgttt
    36301 tctttcatga ttttttcgct cctttgccct gctccatttg ctgaaccaga gttgatttgt
    36361 cgatttacaa cattaacatc ttaacatgtt tcagtcaagg ttgcaattat cttttcagat
    36421 ttctggtcgg atttttttta ccaaaggaga catcttgaaa ttctttgaaa aattgctgat
    36481 tgttttttgt tttacgcaaa aagcggatca gttgttccgt atattctaat gaatctccag
    36541 tcatattttt tcgaagtttc caaatttctt ctaattcttc acttggcatc aataattctt
    36601 cttttcgggt acctgatttt ttgatatcga tggctgggaa gatccgacgt tcagataatt
    36661 cacgtgaaag ttgtaattct agatttccag ttcctttgaa ttcttcatag atcacgtcat
    36721 ccatacggct tcctgtatcg actaaagcgg tcgctaaaat cgtcaaactg ccaccttctt
    36781 cgatgtttct tgctgccccg aaaaatttct taggtttata caatgcggct gggtcaatcc
    36841 cgccactcaa tgtacgtccg cttggtggaa cgactaagtt ataggcccga gcaagtctag
    36901 taatactatc catcaagata acgacatctc gtttatcttc gactaaacgc attgcgcgtt
    36961 ccaatactaa ttcagaaact cgagtatggt tttgcggctg cagatcaaat gtagaagata
    37021 ccacgtcgcc tttcacgctt cgttctagat ccgttacttc ttccggtcgt tcatcaatca
    37081 gtagaacgat taattctact tccggatagt tttcggtgat accatttgcg atttctttta
    37141 atacagaagt tttccctgct tttggcggtg cgacgatcaa tccacgctga ccaaaaccga
    37201 ttggagaaaa gatatcgatc attcgggtag atagtctttg cggtgttgtt tccagttgaa
    37261 gctgtctttg cggataaaga ggtgttaagg ctggaaaatg cggacgttct tttgcttctt
    37321 ccggatcttt tccattaacg ctttccacgt gcatcaaacc ataataacgt tccgattctt
    37381 taggtggacg ggcttttcct gccactttgt ccccatttct caaactgaat cgtctgattt
    37441 gtgaggcaga aatatagata tcttctgcac ttggtccata attgattggc cgtaaaaaac
    37501 cgtaaccatc ttgaccaacg atatccaata ttccttccat ataataaaat ccttgtttct
    37561 ctgcttgtgc acgaatcaca gctagagata attccttttt attcatctta ctgtaataag
    37621 gaattttaaa ttctttggca taactataaa cttcttttaa cgttttgttc tctagttctg
    37681 ccattgtcaa ataatcgctc attcagctcc cacttcctct atcatttcaa ttgatgcccc
    37741 taaggctgtt agtttctcga taatatgatc ataaccgcgt aagatataat ctacattgta
    37801 aatcgtcgtt gtcccttctg ccaatagtcc agcaatcact aaacaagcac ctgcccgaag
    37861 atcagaagcc acgacttctg tgccgtgaag tttatttgga ccattcacga tgatcatatt
    37921 tccttccacg gtaatatcag cgcccattcg tgctagttcc ggcacatggt tcacacgttt
    37981 cgcatagatc gtatcgatga tttctgaagt gcctgtagtc aacaatagta acgcagtaag
    38041 tggctgctgc agatctgtgg caaatcctgg ataaggatag gtcatgatgt ttgcgggttt
    38101 taattctttc gaggggtaaa cttcgatttc atcttcgctg attttcatat ggacaccaat
    38161 ttcctgcaat tttgctataa agctttccaa atgttcaaag atcacatttt tcactttgat
    38221 accatttcct actgcagctg ctaatgctag ataggttcct gcttcgatgc ggtcaggaat
    38281 catgaaatgc cgacagccat gtaatgtttc gactccttcg atacggatca catctgtacc
    38341 cgctcctcga acttttgccc ccatattatt cagtaaagtt gccacatcta tgatttccgg
    38401 ttctcgtgca gcgttttcaa ttactgtctg tccttttgct tttacagcag ctaacatcac
    38461 attgatcgtt gctccgactg aaaccacgtc catgaaaatc cgattgccac gcaatccttt
    38521 attttctgtc cgtaaataca tggcaccatg ttcatttgtc acttcagcac ctagagcttc
    38581 aaatcctttg acgtgtaggt caatcggtct agggcctaaa taacagccac ctggcaggcc
    38641 tactaccgct tcgccaaatt taccaagcag tgttcccata aaataataag aagcacgcaa
    38701 actattgatt tttccgtgcg gcatcggaat cgatacgact tctcgaggat cgatttctag
    38761 tatgttgttg gaaaaatgga cagttgcgcc catgatctct aaaatttcaa ttaatgaatg
    38821 aacatcttgt atgtccggca caccttccaa gatcactggg gaatcagcta agattgcagc
    38881 tggaatcaat gcgactgcac tattttttgc accgctgatc gttacttcac cagctaaagg
    38941 tcggtttcct ttgattacga tttttttcat ctgtttccac cttgtttatt aatctttttt
    39001 catggatatg cttgtatttt atcatagata aaaaaaaaga aacaagcatg gtctgccgtt
    39061 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
    39121 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
    39181 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
    39241 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
    39301 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
    39361 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
    39421 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
    39481 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
    39541 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
    39601 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
    39661 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
    39721 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
    39781 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
    39841 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
    39901 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
    39961 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
    40021 aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta ttttaccaaa
    40081 catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa gttttttgca
    40141 aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc tatctttttt
    40201 cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc ttatcccctt
    40261 ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt gatctgctaa
    40321 tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag aaatcaattc
    40381 tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga tttctactaa
    40441 acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca attgacgata
    40501 tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg ctccatcata
    40561 tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa ggcgtaaagt
    40621 tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat tgtttgctgt
    40681 atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac gtgcaaattc
    40741 gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat tttcgcgagc
    40801 ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac caggaaccaa
    40861 gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt cttgtgcttt
    40921 gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca atgcttcaac
    40981 tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa tccgtacttt
    41041 tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt ctgcttctgg
    41101 tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt gtaaaactaa
    41161 tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg gttcgacatc
    41221 acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg tacgtaccac
    41281 caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct gcgttggttt
    41341 agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga tatacataac
    41401 attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg gcaaagattc
    41461 tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag tcattttagc
    41521 tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt gaacagttgc
    41581 tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt ttcctgtcgt
    41641 cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc ccagatccaa
    41701 atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg ggctcatcgt
    41761 ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta agccgcggtt
    41821 tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg aaacgacacc
    41881 gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat ttatgtgcag
    41941 gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg gagccgaaat
    42001 ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa gctgactaag
    42061 ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt tcgatatcct
    42121 ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc acataataag
    42181 cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt actgaaaaat
    42241 tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa tatcccacct
    42301 aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc gtagaaacaa
    42361 tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca cagttctcgg
    42421 atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc actaattttt
    42481 tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa ggtaacgaat
    42541 cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt ctaaataaac
    42601 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca tgagaagctg
    42661 tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc tctggataaa
    42721 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagcc
    42781 attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa cctcttggta
    42841 aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa aacaagaaaa
    42901 gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca caacctctcc
    42961 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    43021 gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt cacagcctct
    43081 aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc cactcggaaa
    43141 cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt gagaagatat
    43201 gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata gcattatgga
    43261 tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc attacataaa
    43321 tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct agtcctgctt
    43381 gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac cgcaattcca
    43441 aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga ttacggccac
    43501 tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa gaattgactg
    43561 ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct aagaaatgat
    43621 aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt ccttcatcat
    43681 tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac ggtttcggca
    43741 tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt gacaacgagc ttgaaggttt
    43801 ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca tgctgtcctg
    43861 gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat tccaacacga
    43921 tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa ccacctttat
    43981 cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag aattctggtt
    44041 caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat gctcgtttca
    44101 aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata tcacaaatta
    44161 aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg gagagatctg
    44221 gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa gaaccgtcaa
    44281 acatcatttt attttctaat actttgtcga tctggctcac tggaacttct acatttttga
    44341 tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct tcctcaatga
    44401 ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc ttcctttttt
    44461 aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga cctgttttgc
    44521 ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca tgatctgtca
    44581 acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat acacgtttga
    44641 tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac atatcaatat
    44701 cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact aaatcttgtt
    44761 cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact gtaccaattg
    44821 gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc ctttcgtttt
    44881 ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat gttaggtttt
    44941 ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca aaaaaggtat
    45001 ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta tcctttatta
    45061 gtgtttttat t
//
LOCUS       DAZUKT010000013        41711 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000013 DAZUKT010000000
VERSION     DAZUKT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.013"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(281..373)
                     /locus_tag="ACG816_000724"
     CDS             complement(281..373)
                     /locus_tag="ACG816_000724"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4919636.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
     gene            complement(644..1624)
                     /locus_tag="ACG816_000725"
     CDS             complement(644..1624)
                     /locus_tag="ACG816_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4919637.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1637..2515)
                     /locus_tag="ACG816_000726"
     CDS             complement(1637..2515)
                     /locus_tag="ACG816_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFS4919638.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2531..3331)
                     /locus_tag="ACG816_000727"
     CDS             complement(2531..3331)
                     /locus_tag="ACG816_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4919639.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(3606..3755)
                     /locus_tag="ACG816_000728"
     CDS             complement(3606..3755)
                     /locus_tag="ACG816_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919640.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            4723..5049
                     /locus_tag="ACG816_000729"
     CDS             4723..5049
                     /locus_tag="ACG816_000729"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4919641.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            5057..5965
                     /locus_tag="ACG816_000730"
     CDS             5057..5965
                     /locus_tag="ACG816_000730"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4919642.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6591..6845
                     /locus_tag="ACG816_000731"
     CDS             6591..6845
                     /locus_tag="ACG816_000731"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFS4919643.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            7387..7545
                     /locus_tag="ACG816_000732"
     CDS             7387..7545
                     /locus_tag="ACG816_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919644.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7617..8843
                     /locus_tag="ACG816_000733"
     CDS             7617..8843
                     /locus_tag="ACG816_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4919645.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(8932..9324)
                     /gene="rpsI"
                     /locus_tag="ACG816_000734"
     CDS             complement(8932..9324)
                     /gene="rpsI"
                     /locus_tag="ACG816_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4919646.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(9340..9768)
                     /gene="rplM"
                     /locus_tag="ACG816_000735"
     CDS             complement(9340..9768)
                     /gene="rplM"
                     /locus_tag="ACG816_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4919647.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(10302..10769)
                     /locus_tag="ACG816_000736"
     CDS             complement(10302..10769)
                     /locus_tag="ACG816_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4919648.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10889..12427)
                     /locus_tag="ACG816_000737"
     CDS             complement(10889..12427)
                     /locus_tag="ACG816_000737"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4919649.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            12507..13193
                     /locus_tag="ACG816_000738"
     CDS             12507..13193
                     /locus_tag="ACG816_000738"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4919650.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(13394..13921)
                     /gene="msrA"
                     /locus_tag="ACG816_000739"
     CDS             complement(13394..13921)
                     /gene="msrA"
                     /locus_tag="ACG816_000739"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4919651.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14005..14682)
                     /gene="pgmB"
                     /locus_tag="ACG816_000740"
     CDS             complement(14005..14682)
                     /gene="pgmB"
                     /locus_tag="ACG816_000740"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4919652.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(14679..15807)
                     /locus_tag="ACG816_000741"
                     /pseudo
     CDS             complement(14679..15807)
                     /locus_tag="ACG816_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(15807..17990)
                     /locus_tag="ACG816_000742"
     CDS             complement(15807..17990)
                     /locus_tag="ACG816_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4919653.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17987..19012)
                     /locus_tag="ACG816_000743"
     CDS             complement(17987..19012)
                     /locus_tag="ACG816_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4919654.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19057..19884)
                     /locus_tag="ACG816_000744"
     CDS             complement(19057..19884)
                     /locus_tag="ACG816_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4919655.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19881..20948)
                     /locus_tag="ACG816_000745"
     CDS             complement(19881..20948)
                     /locus_tag="ACG816_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4919656.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20974..21810)
                     /locus_tag="ACG816_000746"
     CDS             complement(20974..21810)
                     /locus_tag="ACG816_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4919657.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21807..22676)
                     /locus_tag="ACG816_000747"
     CDS             complement(21807..22676)
                     /locus_tag="ACG816_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4919658.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(22746..24029)
                     /locus_tag="ACG816_000748"
     CDS             complement(22746..24029)
                     /locus_tag="ACG816_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4919659.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24043..25728)
                     /locus_tag="ACG816_000749"
     CDS             complement(24043..25728)
                     /locus_tag="ACG816_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4919660.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26003..27028
                     /locus_tag="ACG816_000750"
     CDS             26003..27028
                     /locus_tag="ACG816_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4919661.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(27623..31276)
                     /gene="rpoC"
                     /locus_tag="ACG816_000751"
     CDS             complement(27623..31276)
                     /gene="rpoC"
                     /locus_tag="ACG816_000751"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4919662.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(31355..34972)
                     /gene="rpoB"
                     /locus_tag="ACG816_000752"
     CDS             complement(31355..34972)
                     /gene="rpoB"
                     /locus_tag="ACG816_000752"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4919663.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            35700..36668
                     /locus_tag="ACG816_000753"
     CDS             35700..36668
                     /locus_tag="ACG816_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4919664.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            36686..37591
                     /locus_tag="ACG816_000754"
     CDS             36686..37591
                     /locus_tag="ACG816_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919665.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            37588..38394
                     /locus_tag="ACG816_000755"
     CDS             37588..38394
                     /locus_tag="ACG816_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919666.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            38551..39519
                     /locus_tag="ACG816_000756"
     CDS             38551..39519
                     /locus_tag="ACG816_000756"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4919667.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(39836..40360)
                     /locus_tag="ACG816_000757"
     CDS             complement(39836..40360)
                     /locus_tag="ACG816_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4919668.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            40647..>41711
                     /gene="scm"
                     /locus_tag="ACG816_000758"
     CDS             40647..>41711
                     /gene="scm"
                     /locus_tag="ACG816_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4919669.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        12024 a         8775 c         7666 g        13246 t
ORIGIN      
        1 taaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt
       61 cttcgccaac acaatttgac gaagagcctt ttctttcagg aagacttcgg taatttttag
      121 gagttctacc tattctttta accgtttatt ctctttttct aagcgtgtaa tttcatttct
      181 gcgaatggat ttcatgactt cctctaccga gaaaagtacg ttatccatat ttttcaactt
      241 cttgaactca tcaatacacg ctattagagc gagcttctag ctatagacta acttctttaa
      301 cagaaaaccc ttcttccgtt accagtttta cagcggtaat tttaaatatt tcatcaaatg
      361 aacgtcttgg cataatccag atcttcgctt tctcttaatt tggtgtcaat tttagtataa
      421 cccgtccaac ttatttttta actaagtgcc cggtttttct tgacaactcc acccaaaagt
      481 tagatttttt ggtctaagtt ttagggtcgc ttcattacta gaattcaaca agagatgttt
      541 tttattttat ttaaattatg gggtcagttt ccatttgatt acaaacttct ttgttttctt
      601 tttttaaacg caataataaa ggttttaatt tcagcctttt taattagaaa atcaattttc
      661 ccaaattatt ttctttagct ttcttaacga tttctgtcgc aactaccaca tctaatacag
      721 ccgaaccaac tgttttgaag atcgtgattt gttctccact tgtccgaccg atcaattctt
      781 cattgatcag ttggcctagt tccccatgat agctgtctct ttgaatatac ccatcttgca
      841 gcggtgaaat gaagtcccca gcttctttta gaacaccgtc catggtatca aaaataacaa
      901 catctgcggc tttgattatt tctcgtggaa tttcacacat ttccggtgtg taagcaccga
      961 ctccattgat gtgcgcacct tttttgaccc actcagcaga gaatgtcgca cgttttgaag
     1021 tggtcacact tgtaatgatg tctgctcctt caacgcattc ttgactggtt tttgttgggc
     1081 gcatcgttac agaaaattgt tgcatcattt tttcagcaaa ttggctggca cgctcaaaat
     1141 caatatcaaa aatccgaact tcttctaatt tacgaacggt caacattgct tctaattgtg
     1201 actgtgcttg tccgccggtt ccaatcagcg cgccaatttt agcatcttct cttgcaagaa
     1261 gttctgttgc tgctccttga acagcgcctg ttcttaattg tgtcaaataa gtaccatcca
     1321 aacaagcgga taccataccc gtttctggat ccaatacgat catcgttgct ggtacacttg
     1381 gtaagttttt cttgatgttt tcaggataca cggaaacgat tttgacacct agcgcgcctt
     1441 cgccaccttc tacataagca ggcatatata agctttgtcc gttgcttttt ggcacatcga
     1501 tattggtgcg aagaggcaca cttgcctttc ctgctgaata caatgacagt gcttttttgt
     1561 ccgcatcgat ggcttctctc attgaaaaac ttttgataat atcttctttt ttaagaaata
     1621 acatttgaat cgctccttaa ttagctgctg taactttttt taattgactg cccaacatcg
     1681 tatatgcact gtatagtaag accaaaatca cgatccattt caggatatag gtatctaaag
     1741 atttgactag gaatacagcg accaataccc ccaatacacc gaaggttgaa gtaaaaagtg
     1801 tgatttttct gctgtattca ttaaatttaa tgaattgaac actgccgatc ggtactgaga
     1861 aggtacaagc ccccatcata ataggaaagg ctgcaactgg attcaagcct agcaagtaga
     1921 cagttaccat cgttaacgca taagaaccaa tgccgatatt gttcaatgca ccatagacaa
     1981 agagtaaaaa tcctgccaag attaattttc cgccatacaa ttcactggct gttccgtttg
     2041 atggaatcca gttcatttgt cctgcaaaaa tcaacaatgc agcaatgacc aatcctatgc
     2101 cgacaaacag tttgatcgtt ttctctggta acttcacgac aaatcttggt cctaaatatg
     2161 caccgataat ttggcagata atacaaacag ctagtgtctt gatccctacg ttgatggcag
     2221 tgatgtaaga caatgccatg acggctaccg gtactacaca ttgggtattc aacgttccgg
     2281 gtagtttttt catgctgacc catttcaact ttggataaag gacggttccg atagcaaagt
     2341 cggaaacgcc aaatgttgaa agaaagaaga tgataaatga cgtgaagggc aacagacgca
     2401 tatcccctgg ttcttctttg aaattttgtt tgtttttgaa aaggtctctt agaaagtaaa
     2461 taataaaata tagattgatt gcgacaatta gtgtcagcaa aatattccga atcataaaaa
     2521 attcctccta ctagtaaatc tttctgattc cctcaaagcc ctcaccatat tttgctttaa
     2581 gttcttcagg agattctgca atcactttta gtgtatatgc atcatgtttg acatattctt
     2641 ttgctagatt gaccatggcg tggttttttg tccaagtgac atcggttgat tgcagttctt
     2701 cgttaatggt accaaacacg acttcttgct catcggcgac gatgttgatc catcttgctc
     2761 caaagtcagc aagtttgtct gtttcaaaac catgaatata atacttttct aagggaagat
     2821 catattcatg ggtagaactg aataaaatca gtacaaactt atcaactctt ttttcagctt
     2881 gctgcaatgc ctttagtagt gaatccgtga aattctcatg ccaaacttga atgagcaaac
     2941 tttcttttgc gtttttcacc aaatgctcta ctttatccag aacatactcg ataccatcaa
     3001 ctttccacag catttcttct tcatcttttt ctttgatcat ccctagattc ttggtaagaa
     3061 aagaaagatc atttttaaca cttttttcta atttctctaa aaactcattt gccgagatgg
     3121 catgataaag tttaggttct gatttattaa cgacaatcaa attctttttt aatagttttt
     3181 ccaaaatatt atatactttc gagcgtggca cacctgagat tttacttact tcatatcctg
     3241 taagattacc tttttctaat agtgtggtat acactcttgt ctccatctct gaaaaatcat
     3301 attttttcat gatttgaata atcgtttcca ttgattaatc ctcactttat ttttagtagc
     3361 tactacggta acaactataa attctttttg attatctgtc aagagttagt tcaataacac
     3421 acatataata attagcttaa aaacaatctt tttattaaaa agaaacatta taatatgaat
     3481 gtttgtgaaa aaataataca acttagtcat tctttacaac gatttataga aaaacaacct
     3541 ctttaaatat aaaaggtacg ggttactgat tacttgtaaa tctttacgtt ccagttttat
     3601 tctttctaaa aaatatactc acttcttttc ttagacttct ttttttgatt ctcctcttta
     3661 tggtcttgtc cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa agcatttcta
     3721 ggttttggac aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga
     3781 tatcatcaca ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag
     3841 atttcactgt gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta
     3901 atctaagcga tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg
     3961 gaaaaacttt tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttgttttgtc
     4021 ctaccacacg ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg aaaatgacct
     4081 ccaaaaacaa aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc
     4141 gtccgcctcg gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat
     4201 aagataaatc atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg
     4261 taaaaactta tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt
     4321 acccacctgc acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc
     4381 attataatga ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa
     4441 ggcaccaatg aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac
     4501 cctttccttg caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta
     4561 tagcggcaac cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc
     4621 gcaaacccgt agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc
     4681 agtccctacc aaacctgtac tattttttta caggaggttg ctatgttcaa aatctttcgt
     4741 aaaaattgtt gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca
     4801 gatgcgaatg gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc
     4861 aatgaattag ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc
     4921 ggtaaatttt ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct
     4981 catccgaagt acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg
     5041 gatttgtgat ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat
     5101 ccgccagctt cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga
     5161 aaagaatcgt gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag
     5221 tgatgtgttc ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga
     5281 atcatttgat gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca
     5341 agcggcagtt gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa
     5401 ccatattaac gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc
     5461 agaacccttc tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc
     5521 aataacagcg attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc
     5581 gaaacacttt atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac
     5641 aaaatcaaca cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc
     5701 caatgctctt ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa
     5761 agcgaatcgt ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat
     5821 ctggaatgtc ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc
     5881 tgcgaaagaa gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg
     5941 atataccatt atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg
     6001 ttgtcttcca tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg
     6061 tatcttcaaa tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct
     6121 tagcatttaa gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc
     6181 tcctagtata taggcagact gccaattata actgattcaa tccacattat gataaaatcc
     6241 accttttcgt ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg agtcttttca
     6301 ttcagtgctt tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt
     6361 acaaaatcag aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg
     6421 caacagattg gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg
     6481 ctgtttccca gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag
     6541 caattgacta cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat
     6601 caaatcaaaa atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg
     6661 actgtatcat aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat
     6721 tggacagttt tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct
     6781 cgactcttgt ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa
     6841 aatagaaaag agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct
     6901 ttgtggatcc attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg
     6961 tatttgataa tttatcttaa caaccagata attttattca gcaccgtatt atctatattg
     7021 aattctcgga atcaagataa atttttttcc gtttttgttt cttttttaat cttcttcttg
     7081 ttattaatag tgattaggat gatattccta tattgatctt tgataattag atataatttt
     7141 tgaataacgt tccctgctat tcgagctaga aatacaagta cgccatgacc tagttagcga
     7201 caactactta gagatttctt ttgttctggg acaacaaaat gccatgacaa tagcagtaaa
     7261 taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta
     7321 cccaaagcgg ttcaaaaatt ttaaactatt attttaggag atgattttat aaaatcgaaa
     7381 gcctacttga ataaaaaaca agcctgtgaa tttctaggaa ttgataataa tactttagat
     7441 aagtggattc gtgtatataa ttttccagta ataaaaattg atggcgtagt tcgctttgac
     7501 gtagaggata tcaaagattt catgaataca ttcaaaaaaa attgaaattt tcttctcgtc
     7561 acatatacta taaatagcga aatgtactgc cttacttttt aaaggagtga ggaagtttgc
     7621 ctacaaagct ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag
     7681 aaatcactgg aattgcatca gagaagtatc aaaaagtatt tccaactagg caattagcta
     7741 ttaaggccga aagggaaatg aaaaagaaga ttgaaacagt tttaagagag gagaatgcaa
     7801 actcactaga attgaaagga aaaataaaat ttaaagattt ttatgaatca aaatggcttc
     7861 ctagatatga actaggtcaa actactaggt caaacagggc tccaagtaat gtaacaatta
     7921 gtaatactaa ggatatattc cgattgcaca tccttccaat gtttggtgaa tatgcaatga
     7981 attatctgaa ttcaaatact gagataatat cagatgaatt aacaaagaaa tcgaaagaat
     8041 acgcaaatat aaaaattatc aaaggctatg ttcgctctat gtttgatatt gccgaaatac
     8101 tatgttatat cgaattcaac cgaactacca aaattattca atgtattacc gcgcctaaga
     8161 aaatggcttt agaagaaaaa aggataaggg aagggaatca agcattatca tcagaagagc
     8221 tgactgattg gatagaagca gtaaacaatg acttaaataa tcacttgctt acacttcacg
     8281 attacacact tttcatgctt accttatatc taggtgatag aaaaagtgag acttatgctc
     8341 tgcaatggaa atatattgat tttaataaaa atacggttcg tctcaaatat gctttagata
     8401 aatatcaaag aaagacacat acaaaaggtt ggaaagacac cgttattcag gcaccagaag
     8461 ttgtgatgac attattaaga gaatggaaat cagctcaggc tgaacaattg ttgaaactaa
     8521 aaattataca aacacctgac cagtacctct ttacctattc caaaccttct ggagaagtaa
     8581 attgtcctgt tcacgctgac tacttaaact atcgaatcaa tgcaataaaa aaaaggcatc
     8641 ctgaattggc tcaattatct ccacacaaac ttcgtcatac ctatgcaact atagctaggg
     8701 aaggtggagc aaatatgaat caaatctcta atgctttaac gcactcggat atttctacta
     8761 caaaaatata cgtgaacaca cctgacgtag ttgataaatc tgtttttgaa gcatttcaaa
     8821 aaggattgaa taagtgtgat taacatgtgt tctcaaatgt gttcttatta taaaaaaaga
     8881 cactttccaa ataattgaaa agtgtcctga aatattgata taaaaagaat attaacgttt
     8941 tgagaattgt gaagctttac gagctttttt aagacctggt tttttacgtt cgaccatacg
     9001 tgcgtcacga gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc
     9061 tagcaatgca cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc
     9121 gtttacgtta acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt
     9181 gattacttca cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt
     9241 aattttgcca gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt
     9301 gccgatatat tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc
     9361 taaaacttct ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt
     9421 tgtaaattgt ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat
     9481 caaacgacga gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg
     9541 gtagttgctg tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt
     9601 gtctgcattg atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt
     9661 atttttacca cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt
     9721 tgcgtctact acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg
     9781 cacgttattt tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc
     9841 tctttgtggg gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg
     9901 tttttctgct tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa
     9961 aagggtcgtt tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca
    10021 accccttatt ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    10081 ccataaaaca taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca
    10141 ggcttacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    10201 acggcaaaat atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct
    10261 gatttcacaa cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc
    10321 gctttgccca aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg
    10381 ataaagatat cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg
    10441 taaagatctg ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta
    10501 cgatcccagc ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta
    10561 gaataaggtg aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc
    10621 aaatcatcca tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg
    10681 taccaatgtg tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt
    10741 aatacttctt tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt
    10801 aaaataatag tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt
    10861 cagttcgctt ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca
    10921 gttgagcaaa ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat
    10981 agattttctg atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta
    11041 tcaattcctg tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca
    11101 cagctcctaa tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat
    11161 cagccatcat ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg
    11221 tgagctgatt ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt
    11281 ctagaatcgc cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc
    11341 caaacgcatc tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca
    11401 gaccattggc acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt
    11461 ctcctttttc tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa
    11521 agatgccagc accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat
    11581 aacaaaacgt ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc
    11641 cgctcgttcc tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag
    11701 aaagcggccc gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg
    11761 ctgctgtttt tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt
    11821 gctctattcg aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat
    11881 caaaatatcc agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat
    11941 aaaagatata gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc
    12001 tgttttgcag ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct
    12061 gccgataaaa ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc
    12121 ggttatctgc ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca
    12181 ggccatgcat cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt
    12241 taattacttc ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg
    12301 ccatcccttg tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg
    12361 cttcatctgt gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa
    12421 cgttcatttt ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat
    12481 cttggttttg aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg
    12541 gatcattttt atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta
    12601 gatctcattg tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct
    12661 cgatcatcgt ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt
    12721 tgttggcaga agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa
    12781 gaaatttttc gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat
    12841 tttacttcat ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt
    12901 ggttgcaggc cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg
    12961 tgtgccttcg ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt
    13021 ggagtggtgg cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg
    13081 gaatatccgt tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt
    13141 tcattccttt tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg
    13201 ttgtgacaaa cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg
    13261 acttggagct aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc
    13321 ctcagtatta gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag
    13381 ctttttcggc ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag
    13441 aaccttctcg ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat
    13501 cttctgcagg ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc
    13561 gcccgctgtc ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat
    13621 tatagtagat cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag
    13681 tcggatcggt ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat
    13741 catattcgat ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag
    13801 ttggatttgg tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct
    13861 cttcaaatgg tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca
    13921 tataaaatcc cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc
    13981 ttgtgacaga aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa
    14041 cgaacgtact tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt
    14101 tatgttttca cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc
    14161 ttctgaatct tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt
    14221 gagaaaaact tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc
    14281 aaaataactt gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc
    14341 caatccgatc ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa
    14401 aacggatgtt tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc
    14461 aaaggctgat ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg
    14521 ctctagtgac tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg
    14581 aatggacagc tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac
    14641 aagaacgcca tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa
    14701 tgaacgactt gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct
    14761 tgctgtaaag cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg
    14821 ccaacatctg ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga
    14881 acgccctctg gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca
    14941 tctagacgtc cttcgcctac gattacgagg tctgcttgct gtacccgtat cgaactccag
    15001 taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc
    15061 tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct
    15121 catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt
    15181 ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt
    15241 aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc
    15301 aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc
    15361 ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc
    15421 gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg
    15481 cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc
    15541 tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc
    15601 aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga
    15661 aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac
    15721 ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa
    15781 agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt
    15841 tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt
    15901 attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga
    15961 aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt
    16021 actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca
    16081 tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg
    16141 aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct
    16201 ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg
    16261 attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg
    16321 ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca
    16381 ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc
    16441 ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat
    16501 tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc
    16561 gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg
    16621 actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct
    16681 gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg
    16741 acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa
    16801 gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc
    16861 tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct
    16921 ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca
    16981 ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct
    17041 ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga
    17101 gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg
    17161 ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct
    17221 aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct
    17281 gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt
    17341 ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct
    17401 ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca
    17461 ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa
    17521 ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc
    17581 ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta
    17641 atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc
    17701 atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt
    17761 tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat
    17821 tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga
    17881 tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc
    17941 gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa
    18001 cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt
    18061 gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca
    18121 tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat
    18181 ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag
    18241 ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct
    18301 tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat
    18361 cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc
    18421 gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc
    18481 cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg
    18541 gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa
    18601 gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa
    18661 tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg
    18721 gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt
    18781 gaaagcgatt ggctgtacag acactcacga tatcgggaga aatctcattc agcatttgtg
    18841 taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc
    18901 tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga taataagcag
    18961 catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct
    19021 ctctcctttg cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa
    19081 atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac
    19141 ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag
    19201 tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt
    19261 atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc
    19321 atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt
    19381 agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt
    19441 caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag
    19501 tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa
    19561 tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcact
    19621 tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa
    19681 gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt
    19741 gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc
    19801 ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc
    19861 ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata
    19921 ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc
    19981 acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat
    20041 gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct
    20101 cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct
    20161 ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa
    20221 gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta
    20281 tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg
    20341 gcaccgtatt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta
    20401 gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag
    20461 attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga
    20521 gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta
    20581 taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac
    20641 tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata
    20701 tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag
    20761 aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg
    20821 acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt
    20881 tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat
    20941 tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa
    21001 atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac
    21061 caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa
    21121 accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc
    21181 attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg
    21241 tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc
    21301 aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt
    21361 ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat
    21421 gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct
    21481 agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat
    21541 cccaatcact actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata
    21601 catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc
    21661 tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat
    21721 cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa
    21781 taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag
    21841 acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca
    21901 ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt
    21961 agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct
    22021 agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg
    22081 tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa
    22141 ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca
    22201 actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga
    22261 tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata
    22321 gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg
    22381 cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat
    22441 gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga
    22501 taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa
    22561 ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt
    22621 ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa
    22681 acctccccct gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact
    22741 tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg
    22801 acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc
    22861 gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc
    22921 tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg
    22981 actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg
    23041 tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct
    23101 agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt
    23161 gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa
    23221 gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca
    23281 tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc
    23341 atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac
    23401 aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt
    23461 ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa
    23521 ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga
    23581 acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt
    23641 ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc
    23701 tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact
    23761 tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct
    23821 accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca
    23881 ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt
    23941 tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc
    24001 agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag
    24061 ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg
    24121 tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga
    24181 aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga
    24241 atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg
    24301 aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa
    24361 ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt
    24421 tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg
    24481 cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc
    24541 cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact
    24601 ggtcgttcca tctgggtttt tcttgtagtt tactcgggca ccctctttct tcagctgttc
    24661 cactaattca aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc
    24721 gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt
    24781 taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg
    24841 tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt
    24901 atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc
    24961 gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc
    25021 tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa
    25081 gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc
    25141 tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa
    25201 cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg
    25261 caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt
    25321 actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat
    25381 ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga
    25441 gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc
    25501 tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa
    25561 aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc
    25621 ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc
    25681 agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt
    25741 tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata
    25801 gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa
    25861 taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt
    25921 ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc
    25981 caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa
    26041 tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc
    26101 aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag
    26161 aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac
    26221 gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca
    26281 aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg
    26341 cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga
    26401 tgaaacactt ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt
    26461 cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga
    26521 tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac
    26581 agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca
    26641 cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc
    26701 agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc
    26761 attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga
    26821 tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac
    26881 tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact
    26941 aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac
    27001 agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg
    27061 ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat
    27121 tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt
    27181 gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag
    27241 tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga
    27301 tacttatctc taaaacataa atagaataac agacatgtct actggttttg ttttattcga
    27361 ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat
    27421 ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga
    27481 tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt
    27541 tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt
    27601 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
    27661 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
    27721 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
    27781 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
    27841 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
    27901 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
    27961 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
    28021 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
    28081 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
    28141 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
    28201 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
    28261 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
    28321 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
    28381 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
    28441 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
    28501 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
    28561 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
    28621 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
    28681 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
    28741 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
    28801 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
    28861 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
    28921 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
    28981 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
    29041 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
    29101 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
    29161 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
    29221 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
    29281 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
    29341 agtctttcat acggtcaagc atcttagaag tttcttttac tttgaatcgt ttgaagactt
    29401 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
    29461 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
    29521 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
    29581 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
    29641 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
    29701 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
    29761 cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta acaactggtt
    29821 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
    29881 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
    29941 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
    30001 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
    30061 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
    30121 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
    30181 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
    30241 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
    30301 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
    30361 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
    30421 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
    30481 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
    30541 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
    30601 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
    30661 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
    30721 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
    30781 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
    30841 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
    30901 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
    30961 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
    31021 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
    31081 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
    31141 acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
    31201 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
    31261 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
    31321 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
    31381 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
    31441 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
    31501 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
    31561 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
    31621 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
    31681 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
    31741 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
    31801 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
    31861 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
    31921 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
    31981 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
    32041 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
    32101 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
    32161 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
    32221 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
    32281 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
    32341 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
    32401 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
    32461 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
    32521 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
    32581 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
    32641 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
    32701 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
    32761 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
    32821 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
    32881 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accatcatgt ttacataaca
    32941 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
    33001 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
    33061 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
    33121 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
    33181 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
    33241 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
    33301 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
    33361 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
    33421 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
    33481 atccgccagg tcctaaggcg aaaagacgac gtttgtgtgt caattcgcca agagggtttg
    33541 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
    33601 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
    33661 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
    33721 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
    33781 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
    33841 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
    33901 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
    33961 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
    34021 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
    34081 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
    34141 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
    34201 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
    34261 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
    34321 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
    34381 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
    34441 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
    34501 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
    34561 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
    34621 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
    34681 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
    34741 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
    34801 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
    34861 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
    34921 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
    34981 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
    35041 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
    35101 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
    35161 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
    35221 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
    35281 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
    35341 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
    35401 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
    35461 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
    35521 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
    35581 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
    35641 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
    35701 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
    35761 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
    35821 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
    35881 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
    35941 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
    36001 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
    36061 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
    36121 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
    36181 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
    36241 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
    36301 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
    36361 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
    36421 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
    36481 tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa
    36541 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
    36601 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
    36661 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
    36721 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
    36781 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
    36841 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggagtgc
    36901 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
    36961 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
    37021 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
    37081 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
    37141 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
    37201 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
    37261 tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa
    37321 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
    37381 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
    37441 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
    37501 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
    37561 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    37621 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    37681 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    37741 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    37801 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    37861 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    37921 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    37981 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    38041 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    38101 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    38161 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    38221 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    38281 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    38341 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    38401 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    38461 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    38521 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    38581 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    38641 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    38701 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    38761 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgatgacttc tgaatctacg
    38821 atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    38881 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    38941 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    39001 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    39061 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    39121 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    39181 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    39241 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    39301 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    39361 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    39421 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    39481 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    39541 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    39601 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    39661 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    39721 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    39781 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    39841 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    39901 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    39961 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    40021 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    40081 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    40141 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    40201 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    40261 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    40321 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    40381 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    40441 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    40501 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    40561 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    40621 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    40681 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    40741 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    40801 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    40861 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    40921 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    40981 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    41041 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    41101 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    41161 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    41221 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    41281 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    41341 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    41401 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    41461 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    41521 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    41581 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    41641 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    41701 cagattcatc a
//
LOCUS       DAZUKT010000014        38053 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000014 DAZUKT010000000
VERSION     DAZUKT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.014"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(409..1353)
                     /locus_tag="ACG816_000759"
     CDS             complement(409..1353)
                     /locus_tag="ACG816_000759"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4919670.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1649..2593)
                     /locus_tag="ACG816_000760"
     CDS             complement(1649..2593)
                     /locus_tag="ACG816_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4919671.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2814..4028
                     /locus_tag="ACG816_000761"
     CDS             2814..4028
                     /locus_tag="ACG816_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4919672.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4050..6065
                     /locus_tag="ACG816_000762"
     CDS             4050..6065
                     /locus_tag="ACG816_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4919673.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6148..7521)
                     /locus_tag="ACG816_000763"
     CDS             complement(6148..7521)
                     /locus_tag="ACG816_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4919674.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7547..7831)
                     /locus_tag="ACG816_000764"
     CDS             complement(7547..7831)
                     /locus_tag="ACG816_000764"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4919675.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7833..9848)
                     /locus_tag="ACG816_000765"
     CDS             complement(7833..9848)
                     /locus_tag="ACG816_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4919676.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10027..10332)
                     /locus_tag="ACG816_000766"
     CDS             complement(10027..10332)
                     /locus_tag="ACG816_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4919677.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10421..11452)
                     /locus_tag="ACG816_000767"
     CDS             complement(10421..11452)
                     /locus_tag="ACG816_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4919678.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11659..12534
                     /locus_tag="ACG816_000768"
     CDS             11659..12534
                     /locus_tag="ACG816_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4919679.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12686..13528)
                     /locus_tag="ACG816_000769"
     CDS             complement(12686..13528)
                     /locus_tag="ACG816_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4919680.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13620..15497)
                     /locus_tag="ACG816_000770"
     CDS             complement(13620..15497)
                     /locus_tag="ACG816_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4919681.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15494..16915)
                     /locus_tag="ACG816_000771"
     CDS             complement(15494..16915)
                     /locus_tag="ACG816_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4919682.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16918..20307)
                     /locus_tag="ACG816_000772"
     CDS             complement(16918..20307)
                     /locus_tag="ACG816_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4919683.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20564..21955
                     /locus_tag="ACG816_000773"
     CDS             20564..21955
                     /locus_tag="ACG816_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919684.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22028..22675)
                     /locus_tag="ACG816_000774"
     CDS             complement(22028..22675)
                     /locus_tag="ACG816_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4919685.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22683..23369)
                     /locus_tag="ACG816_000775"
     CDS             complement(22683..23369)
                     /locus_tag="ACG816_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919686.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23375..24448)
                     /locus_tag="ACG816_000776"
     CDS             complement(23375..24448)
                     /locus_tag="ACG816_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919687.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24670..25344
                     /locus_tag="ACG816_000777"
     CDS             24670..25344
                     /locus_tag="ACG816_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4919688.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25349..26689
                     /locus_tag="ACG816_000778"
     CDS             25349..26689
                     /locus_tag="ACG816_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4919689.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26838..27824)
                     /locus_tag="ACG816_000779"
     CDS             complement(26838..27824)
                     /locus_tag="ACG816_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4919690.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28548..29579
                     /locus_tag="ACG816_000780"
     CDS             28548..29579
                     /locus_tag="ACG816_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4919691.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29688..31220
                     /locus_tag="ACG816_000781"
     CDS             29688..31220
                     /locus_tag="ACG816_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4919692.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31359..32171
                     /locus_tag="ACG816_000782"
     CDS             31359..32171
                     /locus_tag="ACG816_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4919693.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32161..32646
                     /locus_tag="ACG816_000783"
     CDS             32161..32646
                     /locus_tag="ACG816_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4919694.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32669..33214
                     /locus_tag="ACG816_000784"
     CDS             32669..33214
                     /locus_tag="ACG816_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4919695.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33287..34930)
                     /locus_tag="ACG816_000785"
     CDS             complement(33287..34930)
                     /locus_tag="ACG816_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4919696.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35059..37287)
                     /locus_tag="ACG816_000786"
     CDS             complement(35059..37287)
                     /locus_tag="ACG816_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4919697.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37320..37799)
                     /locus_tag="ACG816_000787"
     CDS             complement(37320..37799)
                     /locus_tag="ACG816_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4919698.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11092 a         7204 c         6917 g        12840 t
ORIGIN      
        1 ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa
       61 ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa tgataaaaac
      121 cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc
      181 gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta
      241 atcaacagat taaatttcat atgttcatat gtacggatac attttcggac cgattaattt
      301 taaccattct atttatttgc ttacctatta gatatcaata aaaaactgct taacagctat
      361 ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt ataatacagt
      421 gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt cttcttcgtt
      481 gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg gcacacctgt
      541 aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca atacagcttg
      601 ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac catagtaagt
      661 tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag caatcacatc
      721 taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg gtttaccacc
      781 gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta agatatagcc
      841 gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga aacgagttgt
      901 atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt cgttccaagc
      961 gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt cgaatcctga
     1021 tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa ctaaatctaa
     1081 acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat cagcatcttt
     1141 acattcttca tagccacctg accatacagc cacattttca tcgccccaag ccataccatc
     1201 taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca atactaattc
     1261 attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa aacctgtacc
     1321 gacgattact actttacgac ttgttttttt catgaataaa cagctccttt tcataactat
     1381 ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg ttaattataa
     1441 tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac agataaatga
     1501 caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact gaaaataaat
     1561 ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa caattttcct
     1621 tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca ttgggagtac
     1681 atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat ccgatacgat
     1741 tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca aagtcgaatt
     1801 ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat ttattactaa
     1861 gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat
     1921 gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca tctgctgatg
     1981 aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat gtatagagat
     2041 ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata ttttcaatca
     2101 aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta tgttcccctt
     2161 tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt gccaaatgat
     2221 tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa atatcctctt
     2281 gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg tcactttctt
     2341 ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac atataattga
     2401 tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt ttactgatat
     2461 gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt
     2521 aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt
     2581 cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc tcctttttgt
     2641 aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa agctattttt
     2701 ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg ctattgagag
     2761 aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg aacatgctta
     2821 tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg acttctgctg
     2881 gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt tctttatctg
     2941 ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt ttgattagta
     3001 gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta gtgattgcat
     3061 cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta gcttcaagag
     3121 ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg attgctgcct
     3181 tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt caaggatttg
     3241 gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg caatatacct
     3301 ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt gttcctacac
     3361 ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta atcaaaacga
     3421 atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta tataatgctg
     3481 tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa actaatgcag
     3541 cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt tttggctggc
     3601 ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg ggaatcggct
     3661 ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg atcctaacag
     3721 gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc caagcgccca
     3781 agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta ggtactttca
     3841 ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa acacttgctg
     3901 ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt ctagtagtac
     3961 acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa caattgacaa
     4021 caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc
     4081 tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc caacgggaag
     4141 aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg aacgtcaagt
     4201 ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc caaggagttg
     4261 ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact tgtccagctg
     4321 gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg atgtttatcg
     4381 agggagacga agtaaaatca gatattggct ggacagcaag taattttatt gaagaatatc
     4441 cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa atctattatc
     4501 aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc ttattcgatt
     4561 ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat atcgaatcta
     4621 tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat tgctactata
     4681 agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta
     4741 taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt ttatctcttt
     4801 cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg tcagtatcag
     4861 aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa cgagatcgct
     4921 ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt ttttttgacg
     4981 aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc aagcagcata
     5041 tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac cactatatga
     5101 tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca ttaatgagat
     5161 acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa gattggatat
     5221 ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa gtcttattac
     5281 tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca tatggagaga
     5341 acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa caaaacctat
     5401 ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa tctgggagaa
     5461 gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct aacaaagacc
     5521 aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag attactactc
     5581 catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg aatatcgtct
     5641 accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta actttctggg
     5701 aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga gatccttatg
     5761 gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga cgctatttta
     5821 tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct aatctagatt
     5881 catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa
     5941 caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa atgaatggta
     6001 aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca aaaataaatg
     6061 cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt ctgatattgt
     6121 ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa tgtttgttct
     6181 ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga tgattccaac
     6241 tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg gtactagata
     6301 atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg gaaggataaa
     6361 taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg cccctcttct
     6421 accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat gtcctactac
     6481 cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata gtgttcctac
     6541 aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa aaacgatagg
     6601 aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt ggaatgctgg
     6661 aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa
     6721 tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta gttctctttc
     6781 aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca tcaagagcat
     6841 gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta tgtcctctgt
     6901 agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat aaccgatatt
     6961 tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa acggttgaca
     7021 aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata agatcatcca
     7081 caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag aaacataaag
     7141 cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa gtaaaatatt
     7201 cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat
     7261 cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg tttggaacat
     7321 cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca gtactaggaa
     7381 tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct tctgaagaac
     7441 gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga ttgacacaaa
     7501 caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat tcttttgaca
     7561 agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc aaaggtacga
     7621 gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg aagaaataat
     7681 cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac tcgtccacat
     7741 ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa cttgttccta
     7801 atccactttg acatactgca aaaattttca tatcattcgt ctccttttct tatgatcgct
     7861 aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt attagaaagt
     7921 agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc agctaaacag
     7981 atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg ttgaagtttc
     8041 agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg taatgcgatg
     8101 tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat agctttgata
     8161 taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt gattgcttct
     8221 tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa ttcagatagc
     8281 ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt taatacttga
     8341 tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag cgccgacaac
     8401 aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg tacttgattg
     8461 atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag agactttttc
     8521 ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc
     8581 tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa taattgctga
     8641 agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat cacggctctt
     8701 atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa tagtatagtg
     8761 aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa cggaagtaat
     8821 gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc gatcatcaca
     8881 ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg ataaaagaca
     8941 ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc catgcgatgg
     9001 atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac atctccttca
     9061 acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg ttcggaatag
     9121 ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc caacagttca
     9181 gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa actcaaataa
     9241 taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga aatcgtttta
     9301 tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg
     9361 agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt tgcttctctt
     9421 cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata cgacagctga
     9481 caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac tgtcccttta
     9541 gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt cgaataaacc
     9601 atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa tcgagtaata
     9661 tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat acgggtatca
     9721 tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt tacttcgatg
     9781 atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata actaattttg
     9841 tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct tttaaatcga
     9901 ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa cgatttctat
     9961 cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg aattgatttc
    10021 gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt tttctttgac
    10081 atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag gctttgtttc
    10141 ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt caccagagtt
    10201 tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc ctgctaaagc
    10261 cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt tttcaccgtc
    10321 ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta caataagaca
    10381 gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt ccttctgatt
    10441 cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt tcttcatctt
    10501 tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag acacggctgt
    10561 aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca tctatatagg
    10621 tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa
    10681 acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc cctttttttc
    10741 cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt atcgaaattt
    10801 tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct ttccctaaaa
    10861 gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga acttgatcta
    10921 ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca tgcgtatttt
    10981 ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata tcccctaatt
    11041 ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt ctgttttggt
    11101 aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct gcttcctttg
    11161 acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt gccatttttc
    11221 catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc tcaggatcat
    11281 cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc cacactggtt
    11341 cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt gaaaaaggca
    11401 aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc atgagtggtg
    11461 cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat ttataatagc
    11521 tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg aaattaatgt
    11581 tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat cagtaagata
    11641 gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga gttgtttacc
    11701 acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc agtctacatg
    11761 acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat tcgtttatgc
    11821 aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg ggaagttcat
    11881 caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc aagtgaagat
    11941 agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat aaaaaaagca
    12001 caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa caaggcacag
    12061 cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt ccagaataaa
    12121 atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc ttcttggaat
    12181 gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg tcgcatccat
    12241 cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt gctgattacc
    12301 ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat atcgcaggag
    12361 gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga ttacgtatta
    12421 aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg caacttgaaa
    12481 aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag
    12541 atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata tcatcataaa
    12601 aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac gattcgaata
    12661 gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac gattgacgaa
    12721 aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca tacaggaaaa
    12781 atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca tgataaggaa
    12841 cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta caagttagga
    12901 gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc ggttctaccg
    12961 tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata tagaaagaat
    13021 cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt cctcgatggc
    13081 tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct tccaataatg
    13141 aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa tggacattga
    13201 tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta tcctttttcg
    13261 caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct tttgctaatt
    13321 gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca ttttctttga
    13381 ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc agcgcgtcgc
    13441 taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc atcagcagat
    13501 ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact gtttgataat
    13561 aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt gcaaaaagac
    13621 taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag aaggactact
    13681 cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt acctttgtgt
    13741 ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga ttgttcatcg
    13801 atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt cgtttcttcc
    13861 agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact tgtggttgta
    13921 aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga tgggtcgatc
    13981 gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc tccagcaagt
    14041 gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc agtaacgaca
    14101 tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc ttggttagaa
    14161 aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa tttcaatgta
    14221 ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa tccgtctgtt
    14281 tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata taaggcataa
    14341 gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt atcgataagt
    14401 ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa tgggatattg
    14461 acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt caattgaggt
    14521 gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg tgtttggcta
    14581 tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc attatcagga
    14641 gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat caactggcct
    14701 ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg accagtaatg
    14761 aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt gacactttgg
    14821 atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc tgctccattt
    14881 agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc attaccgact
    14941 gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt ataagagcca
    15001 tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc ggcagctgac
    15061 actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt ttgtttttta
    15121 ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac tggtgtacca
    15181 ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt atctcgttgc
    15241 gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa tccttggtat
    15301 tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg taaatcagtc
    15361 attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac tttttttgcg
    15421 ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat aacgtttatt
    15481 tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac cagattgcaa
    15541 agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg tttgtttttg
    15601 gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg tccgcctctt
    15661 tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt aaaatataac
    15721 cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg gaaattgaaa
    15781 aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt
    15841 atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg aatactgcgc
    15901 cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta ctccctgtat
    15961 atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga
    16021 tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg gtcagttttt
    16081 ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg tgaggtttct
    16141 tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt aaactattag
    16201 gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga tagtcaagga
    16261 tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat
    16321 ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt
    16381 taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca ggaagaacaa
    16441 ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca atgaaaagat
    16501 acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg ataccatctt
    16561 cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac gcaccgtttt
    16621 ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag
    16681 aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac gtttgtaata
    16741 aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt gggaaaagca
    16801 atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc tgagtaaaag
    16861 ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt ttcatattta
    16921 gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat
    16981 ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg gtacttttgc
    17041 ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca gaacagattc
    17101 atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag taactggctc
    17161 ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag ttccaggttt
    17221 taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt ctaagtccat
    17281 ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt cttctttttt
    17341 gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc atacattatt
    17401 tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga tttggaattc
    17461 ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga ctcgatattt
    17521 accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt ggctgtcaat
    17581 tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc ctttaagatt
    17641 tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa cttcaacaaa
    17701 aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt ttccatcaat
    17761 ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc ttaatccatg
    17821 cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca atttgacatt
    17881 ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa tagaatctcc
    17941 tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat ttttcgttat
    18001 tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat ctatttgttg
    18061 agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg cttgaccttg
    18121 attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat ttgcagataa
    18181 tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt taatgattcg
    18241 aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt
    18301 aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact ttttgatgtg
    18361 aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt cattggtatc
    18421 aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg gatggaagtc
    18481 ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt cttttcctaa
    18541 atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag tgaccgttgt
    18601 aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga atggttctgc
    18661 gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag cttttttggc
    18721 taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat cgcctttttc
    18781 atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga gaccagctgc
    18841 cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt ctttgagtgc
    18901 catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata gattgttttg
    18961 gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat cttgcgtatt
    19021 agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct gtactttata
    19081 ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc catttggaac
    19141 ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac catttgtccg
    19201 tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca tctgtactgc
    19261 accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat tgattttgtc
    19321 tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct ttacttcacc
    19381 gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga gatcaagagg
    19441 cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca atccgggtgt
    19501 acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg ttcctccata
    19561 ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta taccatccca
    19621 ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc ctgtaggttg
    19681 ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag gaatcgtttg
    19741 attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg ggctgacaga
    19801 attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta ctgacacatc
    19861 tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat cgacgatcag
    19921 cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat cgatagtttg
    19981 tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat aagcatagcc
    20041 atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa tttctttttc
    20101 tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt tttttccaga
    20161 ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc
    20221 tgctattgca gataatggca caaagtttga taaactcaat attatcagca tgaaaataac
    20281 tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt tctgttttga
    20341 cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt attttaatat
    20401 gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac caattggttt
    20461 catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg
    20521 agaaaaacat atgatcaggt tgttggaatt aaactataga aagatggaaa aaatcattcg
    20581 aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac gaattggtag
    20641 ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg gaacggatgt
    20701 aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg taccagctta
    20761 tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat taaatgagat
    20821 tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca tcagccggtc
    20881 ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt tattaaaagt
    20941 gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt tgcgtatctt
    21001 tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc caatcgaaaa
    21061 aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt ttcaggaaga
    21121 aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca tgagagaatc
    21181 ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga taaatatcga
    21241 taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat
    21301 aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa
    21361 tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg
    21421 caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg
    21481 tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat
    21541 gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac
    21601 tctactgaat cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg
    21661 taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag
    21721 tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat
    21781 tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc ttctcgagta
    21841 tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga
    21901 tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat
    21961 caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg
    22021 tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca
    22081 actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca
    22141 tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc
    22201 ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg
    22261 ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt
    22321 gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt
    22381 tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat
    22441 acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca
    22501 tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca
    22561 gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg
    22621 gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg
    22681 attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga
    22741 tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca
    22801 tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct
    22861 gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact
    22921 ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct
    22981 aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca
    23041 actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata
    23101 aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag
    23161 tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg
    23221 aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca
    23281 taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta
    23341 acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt
    23401 ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa
    23461 tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa
    23521 tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa
    23581 aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta
    23641 ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag
    23701 caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc
    23761 caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt
    23821 ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag
    23881 aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga
    23941 cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt
    24001 aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga
    24061 tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg
    24121 cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc
    24181 tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta
    24241 attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc
    24301 aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga
    24361 atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat
    24421 agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg
    24481 acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt
    24541 tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa
    24601 aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga
    24661 ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc caataatata
    24721 aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg
    24781 ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg
    24841 aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt
    24901 ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat
    24961 tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa
    25021 cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta
    25081 aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa
    25141 ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca gatatttgac
    25201 cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac
    25261 ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc
    25321 ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag
    25381 tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat
    25441 tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat
    25501 aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt
    25561 tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc
    25621 atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa
    25681 taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa
    25741 tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt
    25801 ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt
    25861 agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg
    25921 tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat
    25981 gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga
    26041 attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa
    26101 tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct
    26161 cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct
    26221 ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga
    26281 gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc
    26341 aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata
    26401 tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat
    26461 tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg
    26521 attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc
    26581 aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc
    26641 acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct
    26701 aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt
    26761 ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca
    26821 acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt
    26881 gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt
    26941 tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc
    27001 tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa
    27061 gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct
    27121 accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca
    27181 tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt
    27241 ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc
    27301 atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg
    27361 ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt
    27421 gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag
    27481 ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga
    27541 tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt
    27601 ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct
    27661 ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg
    27721 atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc
    27781 ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc
    27841 aaatagattt atgattggta tattgttact tataaattta ttctatagga gacgattgtt
    27901 ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt tgaaaacatt
    27961 tatgttaatg aaagtcatat tctaataaaa tgtcatttgt ttctatatga atttttatga
    28021 aaaatttcat ataatttcca ctcgttgtga tggtttaatt tatccaatcc atcaataagc
    28081 aaacaactta tataaaaaag gattaattct caatataatt gctcctacca tagaataaat
    28141 cttcatgtaa agtaatgaag taccaactat ttctaatact tctttaagaa ttatcccaac
    28201 aatatcgtta gacaaaccat tattgaataa atggagagcc aatcgattct gaaaaaatgt
    28261 tttctaattt ttcggaaata aaagaaagag cctcctgcca ttagaacgaa ccaaatccat
    28321 ttcattgctc atgaagtaac cacatagcta aaaagaggat aaatcaatta aaagatcaaa
    28381 agaatgactg cactaataaa ataactaaaa atgtatcttc ttacttcata tcttttagct
    28441 agtacttagt taaaaatttg cttcctaaga tcaagacaaa ctgtctatct tcatttgatg
    28501 aaatgcggta aaatatagaa aatgaacact attggaggga atttgtaatg gacagcaaaa
    28561 ctagaaaaca actgcaagaa aagttaaagc aacgtacaca cttgctccct tccgtctttc
    28621 aacagttttt agaagaaaat cgttcttccc tttcattagg cagtcgctat gaatacgcaa
    28681 aagattttca tctgtttact gattatttga gaaaagtatt ttctgaagaa gtggatgatc
    28741 agctgattat ccatttagaa aaacaagatt atcagaattt cttagctaaa atctatcgac
    28801 atactcgttc gttccaaact acagcgaacc aagaaacgga gcaattgttt gaaaatagct
    28861 attacgggat ttcaaggaag caatattcat taaatcattt tttacgattt ttgtatcaaa
    28921 aaggactgat tgaagaagag atttctcttt tgggtacaag tttaccggaa acaaccaaag
    28981 cagctccgcg atatatcgat gcagaacttt tcaaagattt tgagcatttg tttatcccga
    29041 ttgaaggctt tcaaacaagc agagaagcta gctttgaaga aaagaataga ccgaggaacc
    29101 ttgcaattac tgccattctt ctgtattgcg gattaaagat ccatgaagta gtagaactta
    29161 aacgtaaaga tgtatctttg accaagcaga ttcttaatct gaatagaaaa gaaaacagac
    29221 tgaataaagt accaatacca aaagaagcga tgccatttct ggagaactat tttaagttaa
    29281 cagcacaaaa tacggacgaa aattcttttg tttttcgttc ctcacatgat cagcaaattt
    29341 ctcctcggac tattcgccaa attttgagca aaattacagt atataaaaac gtatcgccag
    29401 aactatgcag aaaaacatat cgttattatg cagaactata tttagatgat gctgacgcgg
    29461 taaattattt atgtggtaat cgatacctct catcccattc ttttcaagat atctgggaaa
    29521 agatggttga tttttcttat cacgaattag cggcattcgt tcgggtttct tccatataaa
    29581 acaaatgtat gatctttttt tcacaataaa ctatgttatt atataaagga ttatgttacg
    29641 attatcttga atttgtatat tacttaacaa aagataatgg agtgtgaatg atggagacca
    29701 taacgtattc ccctttgaac ttatttacga attttcaaga agctgcagct aacttttccg
    29761 agaccccgat tatctttgat gagccacttt cagctttccc agaattagga cttgaaacaa
    29821 cttacaaaaa atgctgcgaa agtgtgttga aacgggctta tcaattggct catcttggag
    29881 tgaaatccgg agacaaagtt attatttata agagttccgc ttttgacaca taccttctag
    29941 ctgtttctgt gtcttacctt ggtgcagtgc ctgtcatgac ttcttatcat ctaccaacaa
    30001 caacaatgga agtatttatc gatcgtttag aagacccttt cattttattt gatgatgaga
    30061 caaaaaagcg agtgagagaa atttcaaatg gaacaaaatc aaaacagatt cctattcttt
    30121 atttaatgga acaacctgct ttgtctgttt ctcaatcatt tttagacaaa aatgaaattt
    30181 cttatatgac ccatacttct ggtacaactg gtatccctaa acttatttgt cattctgctc
    30241 attctatggg atggcggacg aaatggcaaa aaacagtttt cacaaaaatt tcggaaaaga
    30301 aattaatcgc ttttcacatc tctccagtgc attccagatt caatattggt atctcttctt
    30361 tgatgtccat gggatttcct atgatgcctt tagcaaacgc acaaagttca aaagttgttc
    30421 atatgttgga agctcaccgg ccaattgcac tagagaccca tccgaataac tttgttcagt
    30481 ggcgttttac tgctaaggag catccagaag ctttttcggg tatccgatac tatcactcca
    30541 cttttgatgc tataaacaac cagacaatga agacattttt gcgaacgtcg atggcacagg
    30601 atgcggtatt tcttcaagta tatggccaaa gtgaatgcgg accaatgatt cttaaggctc
    30661 atacactaga gagtttgaaa acaagtgatg caagagatat gggcgttgga ctggaagaca
    30721 tgacaagcgc tcggattaca gattcagttg ggaatgtcct acctgaaaac acagatggtc
    30781 atatacaatt actgtctaaa ggacgggctt tgacttatta caaggaagat gcacggttcc
    30841 aggaaaatgt ttatggcgat tggtgggata gcggagatta cggttttatg gataagcatg
    30901 gccacctatt tttgaaagat cggcaagtgg atttgatcga aacgatcaat agtacattag
    30961 ctattgaaga ctttttatta gattcgttag attttttggc agaagtagtg attgtaaggg
    31021 ataaaaatca ttctccacaa ccgattattg ctttagctcc tgataaagaa atggattgga
    31081 atcgatggtg ggagcaagtc catgaacttc ctcgcttgaa cgtccctatc atccgtgatt
    31141 ttgatgcaat cccgcgaaca gctacaatga aagtgcaacg gcttcaaatc gagaaagaat
    31201 taaaatcaca aaagtcctaa ctatttaagg aaagaaagat ccattttggt ctttcttttc
    31261 tttttgtttt tttaaattgg atgagataaa aatatgccaa ttggctgttt ttcttttttg
    31321 aaaatttcgt tatactctta ttcatggagg aaaatgttat gaaaagaatc ttgacaattg
    31381 gcggttctga tccatttgct ggtggaggaa tccaaacaga tttaaaaact tttgagaatt
    31441 ttggtctatt cggactaagc gccttgactt ctataggggc actagatatt aatgagcgat
    31501 ttatgctgga atctatatct ttggaattgc ttaaacgaca gttagtttcg attgataaaa
    31561 tgacttcatt agatggtatc aaaattgggt tattgaattc aagtgaagca atcatggttg
    31621 ttcgagactt cataaaaaca aaacaaaata tcccaatcat tttagaccct gttttggcat
    31681 tcaaagaaac tgaagctgtg atcaatcaat tgtatatttc gcagctcatc gaagaacttt
    31741 ttccacttgt tacgatgatt acgcctaatt tggcagaagc tgctttactt tctggtcaag
    31801 ctcttccctc ttcgttaaca gaaatgatcc atctcgctaa agaactcact gcttcaggaa
    31861 caaaaaacat tgtgatcaaa ggcggcacaa ggatggctgg agatgaagct gtggatcttc
    31921 tctattcaat tgatcattat gaggtatttt ctcataaaaa aatcgacact acaacagtaa
    31981 acggggccgg ttgtgccttt tcttctgcaa taacagcgaa tcttgtgtta ggggcagatt
    32041 ttaccaaagc agtagcagaa agtaaaaaat ttgttgccca ttgtatttct aatggcgtgc
    32101 tgatgaacga tcataccgga agtgtctggt ctggcggtat tcaaaaagga ggaatgagaa
    32161 atgaaaacta agcaattaac gatctatgct atgctgattg cgttgacagt tgccctttca
    32221 ttaacaattt tgatccctgt accggctacg aatggatttg tgacattatg cgaggccggg
    32281 atctataccg ctgcttttct ttatggtcca acgggtggat tagttgttgg tgcttccagt
    32341 ggtctattga tcgatttgat ttcaggatat ccgcagtggg cagttttttc attagtgatc
    32401 cacgggttac aaggatatgt tgctggttct ttcagcaaat cttctactcg tatgtgggga
    32461 ttagggcttt tattaggaag cttagttatg atcatcggct atggtatcgc cggttggatt
    32521 ttatatgatt ggccggctgg agtggcttct ataccaggaa acatcttcca aaatctgatt
    32581 ggaatgggac ttgcctttcc tataactgca agtcttcaac gtattcaagc acggaagcaa
    32641 tattaattga aaaagaaggt tttagaaaat gaagttgtca gaaaaagaat taaaagacca
    32701 attgactgaa atagttaatg acatattggc tgaggcacac ttgaaaaaag gtgatctttt
    32761 tgttttagga tgcagcacaa gcgaagtcgt tggaggacat attggaaaaa attccagtgc
    32821 ggaagttggt caatggatca tccgtacgtt aaaagagttg ttggatccta aagaaattgc
    32881 gctagctgtc caaggttgtg aacatttgaa ccgagcatta gtagtagaac gaacagtggc
    32941 agaagcaaaa aatttcgaaa ttgtctctgt tgttcctgca ctacatgctg gaggtgcttg
    33001 ttctgttgct gcttttgacc agttcaatga tcctgtcgaa gtggaacacg tagttggaca
    33061 agccggtatc gatatcggtg acacatcaat cggtatgcat atcaagcatg tccaagtacc
    33121 tgtcagacct cgtttgaaaa cccttggaca agctcacgtg acagcccttc gctctcgtcc
    33181 taaatatatc ggtggtccta gagcgaatta ttgaaatgag taattgacag agcaatctga
    33241 aaaacttcct cgcaaagtcc aactaatgaa ctttaacgag gaagttttac ttgtttgttt
    33301 tatataaccg agcttcataa ggtgctagta tgcctttagc ttcaaattct ccgctagagg
    33361 aatttgacaa gagtaatgtc caggactgat ctgcttcttt tggaagttca tagtcagcaa
    33421 gtgattctgt taagtttacc ataataagat attgttcatc ttctaaatat ctttcataga
    33481 cataaagttg tggatgttca tgaagatatt ctttataaga accgtatatc agacctttat
    33541 tggtttgtct aatgcggatc atgttcttat agaattgcag gaccgactct ggttcatatt
    33601 cttgttgttc tacattgatc gttttggtat ttggattagt gatcagccaa ggttcatgtg
    33661 tagaaaaacc agcatattgt tccgaagtcc attgcatggg tgtcctgctg ttgtctctcg
    33721 ttgtttcacg aatgatatcc aatgcttctt ctcttgtctt accttccgca agcagccttt
    33781 tataaagtct tttcgaatct actgcatcaa cttgatcgat cgattcaaag ggcatattcg
    33841 tcattccgat ttcctgtcct tgataaataa atggcgtacc ttgcaaaaag aaatacatca
    33901 tagcgattgc ttttgcagac atagcccaaa aatcgggacg agtatctcca aacactgaaa
    33961 cggcacgagg aacatcatgg ttttccatat ataaagcgtt ccatccgata ccatccaatg
    34021 aagtttgcca agcgcttaat gcatgtttca atttaaggac atctaattgc ccttcttttt
    34081 cttgctgcca gatatggata tgatcaaatt cgaatatcat atcgaaataa ccgtctttcc
    34141 ctacccattg tggtccttct tctgcagtga caccacttgc ttctccgaca gtcatgatat
    34201 cgtactcttc aaaaacatgt ttcaaatcgg tcaaatgttc ttcgattcct gagacatttt
    34261 gaaagggtga gaaagggttc tctgtggctt ttactgaata ttcatctttt tttacatgag
    34321 aaatcgcatc taaacgaaat ccatctatcc ctttatccaa ccaccatcgg atcatattga
    34381 atagttcttc tttcaatttc tcgctttccc aatttaagtc tggttgttct tttgcaaaaa
    34441 catgcagata atactgtcca gtttcatgag aatactccca agcagaaccg ccgaaaatcg
    34501 atgtccaatt attcggttct ttgccatcta ccccgtcgac ccaaatgtaa taatcgcgat
    34561 acgggctttc tttagatttt ttcgattctt caaaccattg atgctgatcc gaactatggt
    34621 tgatgacaag atccataatc actttgatcc ctaattcatg tgcttctttt agcaataaat
    34681 caaaatcttc catcgttccg aatctctctt ctatatcttg atagtcactt acgtcataac
    34741 cattatcgac aaatggagat tgatagatcg gattgatcca gataaagccg ataccaagat
    34801 ctttcaaata attaagtttt tcacggatcc cattgatgtc tccttgacca tcgccattgc
    34861 tatctttgaa actagcagga tagatttgat agcctactgc ttctttccac catttttgtt
    34921 cttgtatcat atcgtttcac tcctttaacg gtccctcttg attataaccg attctatttt
    34981 agaagtatcc ttgttaccgg taactagaaa cgaaaagctc aacgaatctg ctaagtgcgt
    35041 tcgttgagct ttattttatc atagatagcg ggcaatcagt tgaatcattt gcttgggaaa
    35101 ttcattcaag tcagtaatat ctacaaaacg actttcaccg tagatcgact tgatttcttc
    35161 tttgtcttgg ccaatagctg cggagacaaa taggattcct tgacgttcat attctttcaa
    35221 aacagactgg atatcttctt ttgctttttt gcctgtataa tccggcaacg cttttggctg
    35281 accgtcacta atattaagca acagtttggt tgttgcggat tgtctgttta atttttctgc
    35341 tgctaaacga agtgcagcac catcacgatt gttttgcctt ggtttcatag tgactaattt
    35401 ttcatttaac cattgaaatc cgtcttcaaa ttctttataa gaaaacaaag aagtcttttc
    35461 tcttgacgaa cggtctgctg tatctccgta aatcaacaat gggatatcga ctttcttaga
    35521 aaattcggca atggcaatcg ctgctttttt tgccgcttcg atccgatcat cccgtatcat
    35581 tgacccagat tcatcgatcc gtacagctac cgctaaagaa ggttgctcat gtggcgggtt
    35641 ctttttgtca aaatttctca aatcaccata tgctactttg cttgcattga accgctgacc
    35701 ttcgtatttt cctttttggt acgtctctgt ttgttcatta tccagtaact ctaaaatttg
    35761 ccggctaagt gattctacta ttggcattac ttgcatgtgt agctcacgtg cttccggcgt
    35821 ttttccttgg aaagctggtc ggtgaacgat gagaccttct ttttcatgaa tcgtttcctt
    35881 catgatttgt cgcgcttctt tattcagttg cttcttgatt tccttatcat gtgcttccat
    35941 gtcagaagca gacatttctt gagaatcatc tcctgtttgt tgttcttttt gattttttcc
    36001 ttcgatatcg ctactgcgtt ctttttgcat ttgaggagga atctcgtttt tttctattgt
    36061 ttcttcttgt ttctcttctc cctcatttgg ttcttcagca gacaacgatt tcttttcatc
    36121 taagcgcaga atttcttcag cattttcagt gatgtttcgt tcatttgctt cttcggcgct
    36181 aatggttctt tcttttgtat gctcagattg aggatgaccc catttttctt ttctcaatat
    36241 gtcctgaagt tccagtagca atccactgtc agacaaagct tgttcaagaa ttgccaattc
    36301 atctgaatca gtggataatt tatacaaaac ttttggataa atcgactgga gtaaatcttg
    36361 tccttgctta taggtatttg cccaataaaa ataagaacgc attcctgcta cacctttgat
    36421 ggcgttagct tttgcagttt catccaacaa acggataatc tctgccatct ttaagagaag
    36481 ttcttgtttt tggatcatag ttttcgccat cgcacgttct gccatgactt ctaaggcagg
    36541 aagatccatt ttttccgcat gttgcatccg atctcttaaa gattcattta atgggcgatt
    36601 tccttggtaa tttcgatttg tagtgatcac aatgacgcaa tctggatggc gacgaatgat
    36661 tcctgtcggt aaattcaaca tcccatttgg ttcgagcgct gagttaagtg ccacaagaac
    36721 agaggcatct cggatgacag tcggctcttg aatctctaat aaatagcctt tttcaatcgc
    36781 tcgaacgatt tcagaagggt aatagctgta atggattgca tctggataat cagcttcgat
    36841 cctttgaact agctctgcta atttttcttt agcagaaaaa ggatcaatgc cgtaatggtt
    36901 ctggatcagt gtcagtacag cttcaagtgt atctgtttgg taaatagctt ctagtaattc
    36961 ttggtcttgt tccttattag aatctactac aggtaataag gctccgaaaa catctgattt
    37021 gtccatatct gcaaaacagg tcacttttgt atagggaagt tgcagatcag cagatagtgc
    37081 tttggctaat tgtgtcttcc ccgacccggc atctccttcc aaaagtacgt tactgatttt
    37141 catttcagga tcttgccaat tccctttgat ttcggaggcg atccgccatt cttctgcact
    37201 tgtccgatgg cttttcggtt tcttccagat catttgcttt tctagccgtg aaaaggttcg
    37261 gttttctgtt agtgagaaat tctccatgct tctcttcccc ttttgatcat ctctgtctat
    37321 taatatccta gctcatctaa aagacgtttt aaaatacgga atcgttcccc aatcagttct
    37381 ccgtcttttt gtagtgtatc gtaatacaaa atgatgtctt catcgatctt tggatttttg
    37441 gtatctactt ctaccgtcat tcggttccct tgtaatccaa tgatgtctac gactggattt
    37501 tctggatcat aaaatttttc ataacgataa gcatcttgga agtataaact tccttgcgca
    37561 gctaagatcg ctaaatctaa aatacgtgtg attggtaatt cttccgactg tcgtgaccat
    37621 ttttctccag tatgtcgcca taccttgcct gagatatcga cttttccacg atcattccac
    37681 tgagcaaggc ccagtgaaag cccttttgca tccatattta atgcatttct tccatcaact
    37741 ttatcatagt cttctacaac gattactggt ttatgtttta aatttgttgg tatttccatt
    37801 attttaactc ctcatccttt tatttttagt aaataagtaa attagtagtt tactcattta
    37861 ctaaactata tgcgacatta tagaggattg aagtgaggat tgcaaagaaa aagagcttca
    37921 ttgatagaaa taaaaacacc atctcgttct tttacacgct cattagtaac atgtagaaag
    37981 tccaagatga tgttggaata ttttggctct atactttttg atgttggtga acttcaccaa
    38041 catctttttt gta
//
LOCUS       DAZUKT010000015        36979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000015 DAZUKT010000000
VERSION     DAZUKT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.015"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACG816_000788"
     CDS             complement(1..120)
                     /locus_tag="ACG816_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4919699.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACG816_000789"
     CDS             299..1960
                     /locus_tag="ACG816_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4919700.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="ACG816_000790"
     CDS             complement(2030..2377)
                     /locus_tag="ACG816_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4919701.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /gene="opp3b"
                     /locus_tag="ACG816_000791"
     CDS             2461..3402
                     /gene="opp3b"
                     /locus_tag="ACG816_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4919702.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="ACG816_000792"
     CDS             3403..4446
                     /locus_tag="ACG816_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4919703.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="ACG816_000793"
     CDS             4452..5522
                     /locus_tag="ACG816_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919704.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="ACG816_000794"
     CDS             5512..6465
                     /locus_tag="ACG816_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919705.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="ACG816_000795"
     CDS             6477..7487
                     /locus_tag="ACG816_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4919706.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="ACG816_000796"
     CDS             complement(7772..8593)
                     /locus_tag="ACG816_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4919707.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="ACG816_000797"
     CDS             complement(8690..8968)
                     /locus_tag="ACG816_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919708.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="ACG816_000798"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="ACG816_000798"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4919709.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="ACG816_000799"
     CDS             complement(11724..12311)
                     /locus_tag="ACG816_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919710.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="ACG816_000800"
     CDS             12486..13808
                     /locus_tag="ACG816_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4919711.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="ACG816_000801"
     CDS             13963..14739
                     /locus_tag="ACG816_000801"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4919712.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="ACG816_000802"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="ACG816_000802"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4919713.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="ACG816_000803"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="ACG816_000803"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4919714.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="ACG816_000804"
     CDS             16629..17258
                     /locus_tag="ACG816_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919715.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /locus_tag="ACG816_000805"
     CDS             complement(17382..18491)
                     /locus_tag="ACG816_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919716.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="ACG816_000806"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="ACG816_000806"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4919717.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19309..19407
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19445..19981
                     /locus_tag="ACG816_000807"
     CDS             19445..19981
                     /locus_tag="ACG816_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4919718.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="ACG816_000808"
     CDS             complement(20021..21784)
                     /locus_tag="ACG816_000808"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919719.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="ACG816_000809"
     CDS             complement(21787..23502)
                     /locus_tag="ACG816_000809"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919720.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="ACG816_000810"
     CDS             complement(24281..24505)
                     /locus_tag="ACG816_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919721.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24696..27473
                     /locus_tag="ACG816_000811"
     CDS             24696..27473
                     /locus_tag="ACG816_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4919722.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27554..27973
                     /locus_tag="ACG816_000812"
     CDS             27554..27973
                     /locus_tag="ACG816_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4919723.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="ACG816_000813"
     CDS             27985..28455
                     /locus_tag="ACG816_000813"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4919724.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="ACG816_000814"
     CDS             28472..29251
                     /locus_tag="ACG816_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4919725.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="ACG816_000815"
     CDS             29238..30059
                     /locus_tag="ACG816_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4919726.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="ACG816_000816"
     CDS             30076..31125
                     /locus_tag="ACG816_000816"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4919727.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="ACG816_000817"
     CDS             31138..32142
                     /locus_tag="ACG816_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4919728.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="ACG816_000818"
     CDS             32775..33794
                     /locus_tag="ACG816_000818"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4919729.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33837..34718
                     /gene="galU"
                     /locus_tag="ACG816_000819"
     CDS             33837..34718
                     /gene="galU"
                     /locus_tag="ACG816_000819"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4919730.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35018..35395
                     /locus_tag="ACG816_000820"
     CDS             35018..35395
                     /locus_tag="ACG816_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919731.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36001..36615
                     /locus_tag="ACG816_000821"
     CDS             36001..36615
                     /locus_tag="ACG816_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4919732.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        12097 a         6481 c         7565 g        10836 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gccacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    33841 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    33901 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    33961 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    34021 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    34081 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    34141 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    34201 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    34261 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    34321 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    34381 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    34441 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    34501 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    34561 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    34621 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    34681 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    34741 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    34801 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    34861 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    34921 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    34981 cgaatttttt aattataaaa aaaggtgtgt gttcactatg ggaaaattag tcgctgttgc
    35041 acagtcgctg cttgatatac tcaagtggct gatggaagga agcaattgga tatttgtgtt
    35101 gccactattg cttattagtc tatttatcat gtttcgcatg gtcagaaaaa aagaaacgtt
    35161 tctgattttt atctggtatg cagcatccgt tggggttgct accttgatgg ttgaattggc
    35221 gttaggtagt tgggaagctt gcgtatttgc tttatttttt gctgtactgt atctaatcat
    35281 ttatatccca tatatgaaaa gaaagtctcg gataggcaga aaaggtagac atacaactac
    35341 tttttctagt acagattctc tcgaacaaag tagaagaacg agaagaagac ggtaatattt
    35401 agtaggtata cgcaaaaaaa gataaaataa tggataaact attgagatga gagatttatt
    35461 taattggtgg aaaaaagctt ttttcactta tataaaagaa tcttttttaa aggaatgctt
    35521 gttgtatcgg gataagccat tccttttttt atgttattta tatatatata aatcatacca
    35581 atgttttttt gtattttctt gcttgcactt ggataagaga ttacttacaa taaattcaga
    35641 tagaaacagc tgaataaaaa aaggctccct gacccttagt tggcgctaag aatcagggaa
    35701 tgaccagtca cacacataca ccctgaccga tcattttgcc atagtcaatg cagtgcatcg
    35761 acctaattta ttgtacctaa tctagggtga aaattctacc cctatgtaga tattttccat
    35821 ggtctgcacg ttgttttatt tgctatgtca aggtattggg cgtattcgtt caatgccttt
    35881 tttgtgtttg aaacagcaaa gaccacatgt gttactgtct aactactgtt tttttatgta
    35941 cttccttaga ttaggcagct gtttctattc atgtcaagta tagctaggcg ggagagataa
    36001 atgagtttat gttattttga ctattatcac caattggaag agaaattaag gatagaagag
    36061 ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa ttaatcaagg tggtagattg
    36121 cagatatatg ctagccgcac actaagtggt gtagcatttg aattttggga tcaaaccccg
    36181 gatcttgtac caagtatatt gattgaaaat ccagccggag gtgtctatga aacgctagaa
    36241 ggtgcaggac aggcaattat tgatcagctt tctgtcaaat cagaagacat ttttcttctt
    36301 ctttcgaatg aaggaagaaa cccggcaatc atcgaactag ctcagtggat caaagaaaat
    36361 ggtcatccat taatcattgt tactggattt gatttgtcac gaagtatcaa atcgcaacat
    36421 agcagtggtt tgcgattatt tgaatttgct gatttggttt tggacaacca cgccaattta
    36481 catgatgcag tcttgacttt gccaaatcta gatccagcga tctgcggaac agcctctctt
    36541 gcaacgatct ttttgctgca gcaagttttg tattttacag caaaacagct agtccaggac
    36601 acaagtggaa aatagttatc cacatgttga caaagttcct taaaaaaata atgttccaca
    36661 taaaaatgga gcgtttgaaa aaaagaaaac gattattaat gaggattaac aacgactact
    36721 ttttttgttt tagttatttt aagatggatc atctttgatt ttaagcttgt tttgtttaaa
    36781 gtttgttatt tttataagtt gtttcacagt tatgagaaat gagttataag aatatgtagt
    36841 gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag aatccatatg gattctttct
    36901 tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa ctctttacaa agtaattata
    36961 gtacatttgt tatcattat
//
LOCUS       DAZUKT010000016        36919 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000016 DAZUKT010000000
VERSION     DAZUKT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.016"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..1035)
                     /locus_tag="ACG816_000822"
     CDS             complement(16..1035)
                     /locus_tag="ACG816_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4919733.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(1046..1243)
                     /locus_tag="ACG816_000823"
     CDS             complement(1046..1243)
                     /locus_tag="ACG816_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4919734.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(1266..1559)
                     /locus_tag="ACG816_000824"
     CDS             complement(1266..1559)
                     /locus_tag="ACG816_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919735.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            complement(1596..1733)
                     /locus_tag="ACG816_000825"
     CDS             complement(1596..1733)
                     /locus_tag="ACG816_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4919736.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            complement(1735..2181)
                     /locus_tag="ACG816_000826"
     CDS             complement(1735..2181)
                     /locus_tag="ACG816_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919737.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            complement(2184..4370)
                     /locus_tag="ACG816_000827"
     CDS             complement(2184..4370)
                     /locus_tag="ACG816_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4919738.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            complement(4370..5152)
                     /locus_tag="ACG816_000828"
     CDS             complement(4370..5152)
                     /locus_tag="ACG816_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919739.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            complement(5169..6926)
                     /locus_tag="ACG816_000829"
     CDS             complement(5169..6926)
                     /locus_tag="ACG816_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4919740.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(6901..9732)
                     /locus_tag="ACG816_000830"
     CDS             complement(6901..9732)
                     /locus_tag="ACG816_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4919741.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            complement(9729..10517)
                     /locus_tag="ACG816_000831"
     CDS             complement(9729..10517)
                     /locus_tag="ACG816_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFS4919742.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            complement(10529..13648)
                     /locus_tag="ACG816_000832"
     CDS             complement(10529..13648)
                     /locus_tag="ACG816_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4919743.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            complement(13665..13952)
                     /locus_tag="ACG816_000833"
     CDS             complement(13665..13952)
                     /locus_tag="ACG816_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919744.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(14006..14374)
                     /locus_tag="ACG816_000834"
     CDS             complement(14006..14374)
                     /locus_tag="ACG816_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFS4919745.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            complement(14420..14932)
                     /locus_tag="ACG816_000835"
     CDS             complement(14420..14932)
                     /locus_tag="ACG816_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFS4919746.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(14943..15335)
                     /locus_tag="ACG816_000836"
     CDS             complement(14943..15335)
                     /locus_tag="ACG816_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFS4919747.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(15335..15673)
                     /locus_tag="ACG816_000837"
     CDS             complement(15335..15673)
                     /locus_tag="ACG816_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4919748.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            complement(15657..15983)
                     /locus_tag="ACG816_000838"
     CDS             complement(15657..15983)
                     /locus_tag="ACG816_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919749.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            complement(15980..16309)
                     /locus_tag="ACG816_000839"
     CDS             complement(15980..16309)
                     /locus_tag="ACG816_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFS4919750.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            complement(16321..16584)
                     /locus_tag="ACG816_000840"
     CDS             complement(16321..16584)
                     /locus_tag="ACG816_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919751.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            complement(16602..17525)
                     /locus_tag="ACG816_000841"
     CDS             complement(16602..17525)
                     /locus_tag="ACG816_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4919752.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            complement(17538..18140)
                     /locus_tag="ACG816_000842"
     CDS             complement(17538..18140)
                     /locus_tag="ACG816_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFS4919753.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(18267..19193)
                     /locus_tag="ACG816_000843"
     CDS             complement(18267..19193)
                     /locus_tag="ACG816_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFS4919754.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            complement(19198..20697)
                     /locus_tag="ACG816_000844"
     CDS             complement(19198..20697)
                     /locus_tag="ACG816_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4919755.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            complement(20710..21954)
                     /locus_tag="ACG816_000845"
     CDS             complement(20710..21954)
                     /locus_tag="ACG816_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFS4919756.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            complement(21941..22474)
                     /locus_tag="ACG816_000846"
     CDS             complement(21941..22474)
                     /locus_tag="ACG816_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS4919757.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(22501..22725)
                     /locus_tag="ACG816_000847"
     CDS             complement(22501..22725)
                     /locus_tag="ACG816_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919758.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(22732..22944)
                     /locus_tag="ACG816_000848"
     CDS             complement(22732..22944)
                     /locus_tag="ACG816_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919759.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            23294..23485
                     /locus_tag="ACG816_000849"
     CDS             23294..23485
                     /locus_tag="ACG816_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFS4919760.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            complement(23478..23819)
                     /locus_tag="ACG816_000850"
     CDS             complement(23478..23819)
                     /locus_tag="ACG816_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919761.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(24118..24585)
                     /locus_tag="ACG816_000851"
     CDS             complement(24118..24585)
                     /locus_tag="ACG816_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4919762.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            24893..25144
                     /locus_tag="ACG816_000852"
     CDS             24893..25144
                     /locus_tag="ACG816_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919763.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            complement(25177..25464)
                     /locus_tag="ACG816_000853"
     CDS             complement(25177..25464)
                     /locus_tag="ACG816_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919764.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(25461..25670)
                     /locus_tag="ACG816_000854"
     CDS             complement(25461..25670)
                     /locus_tag="ACG816_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919765.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            complement(25667..25864)
                     /locus_tag="ACG816_000855"
     CDS             complement(25667..25864)
                     /locus_tag="ACG816_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919766.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(25861..26187)
                     /locus_tag="ACG816_000856"
     CDS             complement(25861..26187)
                     /locus_tag="ACG816_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919767.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            complement(26184..26465)
                     /locus_tag="ACG816_000857"
     CDS             complement(26184..26465)
                     /locus_tag="ACG816_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919768.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            complement(26478..26696)
                     /locus_tag="ACG816_000858"
     CDS             complement(26478..26696)
                     /locus_tag="ACG816_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFS4919769.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            complement(26693..27004)
                     /locus_tag="ACG816_000859"
     CDS             complement(26693..27004)
                     /locus_tag="ACG816_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4919770.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(27181..27495)
                     /locus_tag="ACG816_000860"
     CDS             complement(27181..27495)
                     /locus_tag="ACG816_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4919771.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            complement(27495..27794)
                     /locus_tag="ACG816_000861"
     CDS             complement(27495..27794)
                     /locus_tag="ACG816_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919772.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(27797..27958)
                     /locus_tag="ACG816_000862"
     CDS             complement(27797..27958)
                     /locus_tag="ACG816_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4919773.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(27973..28332)
                     /locus_tag="ACG816_000863"
     CDS             complement(27973..28332)
                     /locus_tag="ACG816_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4919774.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(28329..29180)
                     /locus_tag="ACG816_000864"
     CDS             complement(28329..29180)
                     /locus_tag="ACG816_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4919775.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(29197..30030)
                     /locus_tag="ACG816_000865"
     CDS             complement(29197..30030)
                     /locus_tag="ACG816_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4919776.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(30037..30723)
                     /locus_tag="ACG816_000866"
     CDS             complement(30037..30723)
                     /locus_tag="ACG816_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4919777.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(30729..31475)
                     /locus_tag="ACG816_000867"
     CDS             complement(30729..31475)
                     /locus_tag="ACG816_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4919778.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            complement(31468..31809)
                     /locus_tag="ACG816_000868"
     CDS             complement(31468..31809)
                     /locus_tag="ACG816_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919779.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(31867..31995)
                     /locus_tag="ACG816_000869"
     CDS             complement(31867..31995)
                     /locus_tag="ACG816_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919780.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            complement(31988..32695)
                     /locus_tag="ACG816_000870"
     CDS             complement(31988..32695)
                     /locus_tag="ACG816_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4919781.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            32740..33219
                     /locus_tag="ACG816_000871"
     CDS             32740..33219
                     /locus_tag="ACG816_000871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919782.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(33460..33726)
                     /locus_tag="ACG816_000872"
     CDS             complement(33460..33726)
                     /locus_tag="ACG816_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919783.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(33737..33928)
                     /locus_tag="ACG816_000873"
     CDS             complement(33737..33928)
                     /locus_tag="ACG816_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919784.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            34219..34641
                     /locus_tag="ACG816_000874"
     CDS             34219..34641
                     /locus_tag="ACG816_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4919785.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            34658..35080
                     /locus_tag="ACG816_000875"
     CDS             34658..35080
                     /locus_tag="ACG816_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4919786.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            35109..35309
                     /locus_tag="ACG816_000876"
     CDS             35109..35309
                     /locus_tag="ACG816_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919787.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            35396..35845
                     /locus_tag="ACG816_000877"
     CDS             35396..35845
                     /locus_tag="ACG816_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFS4919788.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            36147..36284
                     /locus_tag="ACG816_000878"
     CDS             36147..36284
                     /locus_tag="ACG816_000878"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4919789.1"
                     /translation="MTLQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRV
                     K"
     gene            36422..>36919
                     /locus_tag="ACG816_000879"
     CDS             36422..>36919
                     /locus_tag="ACG816_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFS4919790.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPM"
BASE COUNT        10322 a         7760 c         5902 g        12935 t
ORIGIN      
        1 aacgaggggc tatttttatc gttgcggaat atttaaatac cagcgtttat catgaaaatc
       61 ttgtgctccg cctttagtgt tcccttctgg atcattcgtt gcccgcatca ttacatagac
      121 tttcttatta gggaagttgc gcatgttaaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacat ctggtctata tattccatca gcttttactc gagctaattc
      241 tttcccagta ttgtaatcca taatgaagat atactcgtat ttatagttag cgatgtgcca
      301 accagccaca tgtaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagggtag gatttgcagc acctgctcga gttggatcaa tgactggttt
      421 atcatctgaa gttgtcggat tttcatcggt aaatccatga gccaaatcat aggctaattt
      481 ttctttactt acgcccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat ccctggttga ttatacggtg tgtctagtgt
      601 aagtggaata ccatatttca ttgctgaatc tctagctaat tcaacgtatg ccttgtagtt
      661 tttctcaaac gttgctttat catgtgtgtg ttgtaactca atctgcacag gactatttgc
      721 attagcatac gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat
      781 tccgccgtct cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg
      841 cgcttcattg cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tgtactgatt
      901 atttgctact tgtgagctac cttcgtttgg gcctaaatta aattcattgt taatcgtata
      961 ggcaaaccca ttaattggca ataaaaaaag agccgttaat aggctcatcg cagtaatagt
     1021 aattttcttt ttcatttgtt tcctcctatt ttttcaaatt ataagccgac acgccagtga
     1081 taacgcctaa aaatgttgct actgcattga tagtgagtac tgtcatatct gttccattcc
     1141 atccatacgc tttgcctagt gttgcaacta atacagatgc agctggcaat actgttaaaa
     1201 ctgtccattt aatgacttga taatacttat cgggtaaaat catttcttct taactccttt
     1261 cttttttacc gagatctttc tctaaataaa gttttgattt gttgtgtgtg ttctaccaat
     1321 ttttctgtat gtgtatctaa tctttcatcg tgtttcttta gttcttcatg aatcatcaat
     1381 cgatctgatt tgctcgattc taaatcttta gtcagcaaat ctaaattgtg acttactttt
     1441 gaaagagttt cagtaatctt cgagaaagat gcagtgactg gtcttattac taataaaatc
     1501 aaagaaacaa tcgcagtgat tgatcctgcg attgttcccc attctcctaa attaatcatg
     1561 tgacaactcc tttaccttaa ataaaaacgc atcacttaag atgcgctctt atctttatta
     1621 atgatttttt ctgcttcttc gtctgtaatg catagtggaa cgaatagtcg aacttgatcg
     1681 tcagtaaaac agccccaatc atacatcatt ttcacatcgc taaaactaaa catactactc
     1741 acctcccttt gaagctggat ttagttgctc tttaatttct gcaatgtctt tgctattttg
     1801 gaacgaagca agcatcgttt ttgaattgat ttgtgctaaa ccatccgctt tttctttcaa
     1861 tgcagtattt tcctgtttaa tcgctacatc gcttagcatg agtttggcgt tgagctgttt
     1921 taaatcgccg ttctcatttt ctaacgactc atacatcgct ttgagattgt ttaaatcgtt
     1981 gtgatctagt acattcacta aaacaatcca ttggttcagt ttaggatcaa acatctgatc
     2041 agcaatcgtt agtggttccc catcagcacg aattccttcg agtggtggaa catcggtaaa
     2101 tggaacagtc ataaccatat catcaagcac ttgtccagca tactctccac ctgttcgtcc
     2161 gtatttccaa atatttttca tttttattcc tcccctaccc aataattacc atggaatgta
     2221 aaccattcat ttgcagcaaa ttcgctattc gtaataattg ttcctaagtc ttttcgcacg
     2281 tacatcgctt ttgatacact acttgttcct gacggacctg taagcatgcc atgaacaatt
     2341 tttgcgacaa attcatctgg gacttcatca tcaagtggta caccaaaagc ataagctttt
     2401 ttgaacttta ctgtaccatt tacaataact tcttttccgt gtttaataaa tgttgcatac
     2461 ccatcgataa acgattgact gttgtcttta gttaatgtat aaacagtgca atcattttca
     2521 ctgatcactt tatctcctgc tatttgaata ccatcggcaa agtttttcaa accttcaact
     2581 ctctgaggat catacacatt aacagcatca ttcaagcctt tttcagtata ttcaggtgtg
     2641 acatcccaac tgtaatcatt gggattgtta ctgtctttca agccttcacc aaagtattta
     2701 aactgactaa tattcggggt tcgtgtgtcg cctttttcga tcttgagcca gtcaatttga
     2761 catgcacctg ttgttgattg gggatactgg ataatagtta accgtttggg gtagttaact
     2821 ccaaccttct gcggtgtaaa agtcagaccc catgtatcaa ctaacccttc tactggctct
     2881 aagttaccat atctataatc tccactatcc tcgttgtaaa caatgaacgt ttggataggt
     2941 ggtttggttg ccttcatggt aattgtatag gtttgaccta aaacaaattc ttcttccatg
     3001 ttagcttggt ataggctgta ggcgctagat ttgattggaa acgtaacaga cttattagca
     3061 atattctcac ccaaaggcgc tttacctagc cagtaagggt catcaagtaa gtttggctga
     3121 tatggggtgg ctgttgagcc ttcttcgatt ttgatgtcgt acaacttaaa tccgccattt
     3181 attttatccc tgtcaacaaa gctaatactc atataaaatc gatctaaatt tgtgatttga
     3241 taattaacgt tagctgtacc tttgattgtt atttctttcc ctacatcatc tgttgtaata
     3301 tttgtactat ttgcttccaa taatatcttt tctcctggtg atgtacgata caccaaacgt
     3361 aatttatcaa tagctcctgt agttccttca tcaaatcgaa ctttcgcact cagagtatag
     3421 gttttcccac tagcaagctg aggtgtatta atacgcgtaa acataataat gcttcctgta
     3481 ccatcagaag taaaatgtag cttttcgtta tccgaagtta gtgacccgtg gtaaccaacg
     3541 ttaaaatcgc tcgattttag tttggacatt aaattcggat tcccactata atcataattc
     3601 ccgaagtcga tgctgttact gtacatcttt ttcagcttgc cgaggtcgcc gatttgctga
     3661 ttggtttgat tcatcttgtc ttcaactgct ttaagattgt tttgtgccga tgtaacgttt
     3721 tgagaaacgg tatcaatctt agtttgagta gcttgtagtt gctctgttac ctcgtcaaca
     3781 gtttgatcaa ttgcttgtgt ggctccctct acaaattcat ttatttgttg gtttgccttt
     3841 tcaatcgtag aatccacatt tgttaatgct gcgttaacag catctgttgc ttcttgtttg
     3901 actccatcaa gtaattgttg aaagtctgca ataaagtagt ctgctttatc ttgcgctagt
     3961 ccgtcgattg ctgaacgctt catacggaag gtgaagccta agttatcact tgttgatccg
     4021 tctggatact cgatgtaaac ataagcttcc accataccgt tataagcttt catcgcttct
     4081 ggcaagatat atacgacctg tccattcaag aagctttcag tgatgaggtt tttcgtgata
     4141 aaagggattg gctctttcgt gatcattcca tctacttcat catagataaa catcaacaag
     4201 cgcaagttag ctcctagcaa gtccgccggt gtgccgttct gttgttcgac gttgaactct
     4261 aacgctattt gattatcgta tgatttaaat acaagccccg ttgcttgcaa atcgtagtct
     4321 ttcggctgtg taggtacttt gatagagcct tttttgatga catgcgccat tatttcacac
     4381 cttcaatctt agttattttc acgttgttgc tcattgcggg attggaaaca ttgccggatg
     4441 aaatatctaa agctctgccg tttgacatcg ttatctttcg cgcttcgatt gtcagctcaa
     4501 attcgatgaa cgtcactccc gcgtctccat tccataaatt cgcttttgta attcgcgcgt
     4561 atctttgacg tttcacttct tctacaaagt ctccgtcacc gtctgtgtag tggatacgaa
     4621 gcgtttggta acggaaagta tcatctggca gattgactga ttgtcctttt cttaattcgc
     4681 cttcaaaaag aacagaagta gtgtaaacgc gccgccagcc taacgagtta taaccctcta
     4741 ccggttcgct gttatgtgtc attttgatgt aaatttcgcc gctataacga gcaaaagcga
     4801 cgattaattt tctcgaatcg tgatccgcga ataccatcat ttcaacaaaa ctgtcatctt
     4861 caatcccgtc agggttgtct gcgccccatc cggctttggt tgcgtatttg ccgggcggaa
     4921 tgtctaaaat gttcggatat ttttgtggta gcttataatc gcgtaaccaa cgcccacgcg
     4981 cttgcataac tgtttccggt gtagcaaagc ccgcgttgct ttcagtagct aggacgtgcg
     5041 cgtcgtgatc cgagccgtcg taatggatag ccgcttgacg caataaccat ttcacagcct
     5101 cttcataact catttcttca aaaatatttg gtttgctttg aaaatctaac atttaattgt
     5161 tcaccgcctt aatagttata agtgaagtct ctcgcgcgcc ctacgccttt gcttgtcact
     5221 tttcttgttg ctgtgtcaac ttctatttta taaaacgcta attcatccgg tgtatcaact
     5281 tggctagctg tatgagagaa gccgacgtcg caaagaatca tttttactgt tccaaacgcc
     5341 ccttcgactt gttcgtgcca atgcccgcag aaataaccta ctacaacgcc ggcgcctttt
     5401 gtattcattg cgaaggtctt taatccgccg aacgaaccgt cattcggttg tcctaattta
     5461 gaccaatcga tagttaccgg actaccagat ttaaaccctt ctatcaacgt gctaatcatg
     5521 ttttcattgc gaacgggaaa cttgctgaga cctaaaggcg tatgtcctac taacgccaca
     5581 tgatagtttc tcggaacgtt cactaaccat tctccgaaag cgttaatttg tttggcactg
     5641 aaagctcccg gcgctgtatc gctgtaaccg tccacgtact tgtcgcctgt gccgccttca
     5701 taaaaatcac aagtatcgaa acggtaaatt gctgcgtttt tatctgggaa caacacgcca
     5761 ccgtacaaac cgttccaata ctcttcgaag tcagcgttgc ataacattcc ctttcgcttg
     5821 cgccacgcgg ggtcgaaaca agcgtcgtga ttccctttgc agataataac cggtttttct
     5881 tgtcctgcta ccgctgcgtt agtgaagcgt ttgagcgtgc ctaacatgga atgtcgcgcg
     5941 ctccattcat caataatgcc tatatcgctg ccgagtgaac caagtccgcc gtcgatattg
     6001 tctccgccgt aaatcatcac atctgttttg ttccctaatt tttgaaattg agggattgcg
     6061 cgccaatgtc ttaaataaga agcatctttg taaccgacgc catctatacg caagttgtga
     6121 ttatctgcgt gaatgtctgt aatgaaagaa aagttaaatt tgctgttatt aacactgttg
     6181 acaaccgtat tcaaattgcg cggcactagg tcaacgtatt tcatcgtgtc gtaatcaaaa
     6241 aagcgtgttg tttctctaat ttgtgagctg ccgactggca cttggtattt ttggtttaaa
     6301 cgatcagcta atgattcgta gtcgccttta gcttcattca gaatgttaat gatcatacca
     6361 cctggatcga tattttccag tatttcacga ttatcttcta accactgctc ccagtcattt
     6421 ttgccctgat ccatgtaatc tttgaatttt cttagcaaat cctcaaatgt ccacacatag
     6481 ccagagtcac gtaactggct tctagatatt ccagaaatga ctcgataggt aaaatcctgt
     6541 gtgctaaatt gttcactcca agttccatca ccattaagtg atcggaaact gaaatgtgcg
     6601 gtgttttcac caccccattg ccagtcaggc tcacttaagg tgtaaacaag ccttgcttgc
     6661 gctggactgt attcttgtac tttttgttca acaggttggt tttcgccaaa ttttgttgta
     6721 ttaataaaaa acggcactag gccctcgaat gtttttagtt tgccatgttc caccacttca
     6781 acaacgaact tttgcgttaa aacatcccct tgccgaattc gaaccaaatt tattccgttg
     6841 tttggttcgg tggtggatag gaccatttta tgctgcgttt ctgccatgac tatccctcct
     6901 ttagaaatcg atgtagtctc ttgcattgtg gaaatgacct gaagaagatg gataaaactc
     6961 atccataaat tgaaaatgaa gatgttctcc agtggatggt cctgttgtcc ccatcagtcc
     7021 aatttgctga ccagcagtta ccttctgacc ttttgagacg tctactcggc tttgatgcgc
     7081 gtatcctgta tacattccat cagcgtgttt aatcactgtc caatttccat accagtcaaa
     7141 gtaatttgca tcacctgcaa caatcacttc gccatccgct gaagcaaaaa taggtgtatt
     7201 aggatttcca tttacaaggt caataccgtt atgaaattct tgtgctcctg tgattggaga
     7261 agtgcgccag ccaaattcgc tcgtcactgt gattggatcc gcaatcggct tcatatatcc
     7321 ttttgataca ggaatttcca aatctttgaa cttgtcatac cattcttgtg accatgccgt
     7381 ccgttctgga tgtggatcac gtggacgttc aaagttagcc acgaatgctt gtgctgctgt
     7441 gttgatatcg gtcagattca tgaattgtgt ccatgtgtaa ggataagcgc tagttgcgat
     7501 ccattgacca tttggtgcat gccacatcaa caatttaaac tgtgccgtga ttgtgtctgg
     7561 atcatcactg atgccagcct ttgtcattaa attgatcatg tagacacgtc cgctagttgc
     7621 gccagaagaa tcggtccatt gccatacacc ataaccgaac cctggtgcgc cattgccctc
     7681 atcagcggtt ggattagcat ctgattctcc ttgtgcattt ccaagtaagg ctgcagcagc
     7741 ttgttgtgta aaaccggcac ctattgccgt tgcccaaatt tgccaatatc gtttatcccg
     7801 atcagtagtt acttctggtg ggtattgtcc gttccaaccg ttatcgttcc ctccggaatt
     7861 gcctcctcca ttagtgtcaa tttttgtacc gttaacatag agttctttta catctagccg
     7921 tccatctatg gttatatttc cttctgaaaa attgccgtca ccataaagat tatatttgcg
     7981 cttatcagca gtaacatctg ctggaatttg aaaaatagga ttccctcgat cgccaccgtc
     8041 cccagcatta atggaaaaaa tatagtttgg ctctttccag acagcaaagc cgttaatccc
     8101 tttagaacca taagtagata caattgagcc caagcgttct ccatgtacat catctagtcc
     8161 agtagacttg acttgttttt caaaagaaag ttctcccccc tctgccacta attggaaatc
     8221 tttatcgtca aatgttttta aagctacccc ttgcacgagg ataccagaca gaatgcctgc
     8281 gcgaataaaa ttagcattga aagttccatc caacgtccaa gcggtcgtgc tatcaccatt
     8341 gtgtacatct tgaattgttt gccactcacc ttttttacat tgtttgaaag atatccctga
     8401 gttattttgg accataaaaa agcgtgatct aggaatgtta ggtccatcca tataaacagt
     8461 ttcatagatt tctctactat cactaacacc agcttcaatt ccatttaccc aataaataga
     8521 accgccatta tctcctgcac ctcgcataat gtcatcttga tattttccaa tctctgtcga
     8581 ttcgtaaaat gtcattttgc tagattctaa actattaata ttattgacaa tagaagccgt
     8641 ttgttttcta acatcttgtg ttaaattatc ccctagttcg atattcgttt gaccggtaag
     8701 ccgattgaat gtagttttat aaatacgagt tttatagtga taacctttat cgtatctgtg
     8761 aatagtcact gtatttccta tcacatctcc tccagtgact tcagctttga attgtactaa
     8821 cggtctagca gaatcgatta aggttgaata agtattttta agtaaatctg ttggatcatc
     8881 tatatcatca aacactacta cggtttctcg ttttctcatt gatccatctt tttgtggtat
     8941 cccatacttt tgagttgatt ccggatcttc aagccaattt tggcctttag gcttatctaa
     9001 aggatcacca ttcgactttt tccattcaac atcagtgaat tcaattcttc taccgtaacc
     9061 gtcaccaacc tcttcgcctc gcccacgacc tatcattgaa gttgagattg agcttctatc
     9121 tatctctctt acaactgtta atgctttact accatatacg aaacgtgtat tcgattcttc
     9181 gccaatttgt tcatatactt cgatccattt atcctttatt ccatcagaat tcaaagaaca
     9241 cctaaagaca aattccatac ctaaggtttg caattctttc aacgcttctt ttacagagac
     9301 atagtaaaaa gttgcagtta ctgttggtaa cattgcttct acgtgaccaa cgcgccaatt
     9361 tccttcagta aattcaatca atcgatcaag aacatttttt aagggctgcc cactcggcct
     9421 aatatctttg atgatgtaag catctaattc atttgtcgca aatcctaacc ctgtaaactc
     9481 taatgtttca aatgggtcgc taactttagt aattcgatac aacgaaaaag acgactcgtt
     9541 ttcacgaatc gccatatatc ttgcatcctc tatttcttta tcatattttg tcgtaacgta
     9601 aagagtatct ttcattagat cactcttatc agaactaatt tctttttctt gggagacttc
     9661 aatcaaactt cttgtatttt ttcttttaat aagtttttgc aagtgatcaa agaaataaac
     9721 tgtctcactc aaattgtcgc ccccctatag aatattttaa gcttcccatt attgctagtt
     9781 atcttctgac cttgcttaag ataaaagttc tcaaaatcac tttctaaatc aatcatagaa
     9841 gtacaatctt ctccgtttac agttacctgc tcatcggaaa aatcaaaaac caacacgtct
     9901 cctgttttta ttgccgcgtc agttatcgtg atattttgtt ctccgtttgt aattttgatt
     9961 gaattattca tggataaagt aacttcaatt tttcttggtg ttataggaaa ctgtatcgga
    10021 tttccaatat agccatcact aacacactct ttcgtatact ttagtggatc cgcacagaat
    10081 acattaaaac tcgaaataat cgagttggag tctcctggaa cagtatcagt tgatgtatag
    10141 cgaccgtagt aataataatc taattcatca tgaaacctaa tttccacgtc ttcattccgg
    10201 tataaataat tcaacagttc tttgaattta aactgtagtt tttctggatc tctatcttcc
    10261 agcttatatg ttatttttag tgttcttgaa ggtattttct gatttgtaat gattgaacca
    10321 atttgtatct cttgctgttc aacttctaca gaaagcattt ctctaccttc caccgtaagt
    10381 gtttgataac catcaatcaa atcttctaaa tacattccat cgtacatcat ggcagacgtc
    10441 ggaaggaatc gtttagaact agtgagatta atggttgtat ctttgaatga gtacatttta
    10501 ttttctcgtt gatccaaaat attccctcct aaaattccag attaatatct gcaccttcgc
    10561 ccatagcttg tgaaatatcg tccataaaca atctaaacga ttgtcttcca agattgattt
    10621 taaatacagc tggtttagta gagccaccca tatttatttt atgttcaact tgtgcaccaa
    10681 tgtttttatt tgcatttttc aaatttgcag ctatatctac atcagaattt gcattgaaaa
    10741 gttccgcgat aaagtctgcc atacttccaa cagtattctg tacgtcattg aatcctcctg
    10801 tcagaccttt attcagaccg ttcataatag cctgaccagc tggaatcaat agctttctat
    10861 cgtattggat aggtcctttg tgttcacgaa tccaatcacc aatacctcca acaaaatctt
    10921 gcacagattt ccatgcattt tgtaaccctt ctagaaaact atccatgata gcttttccgg
    10981 ctgctagtaa atcgatattt ttcaagttat caaaccagcc agttactcta tcaaccgtgt
    11041 cactaacagc atttactaaa ttatcccatg cctcttgagc accatttact aaattgttga
    11101 aagtatctat agtaccttgt tttaggtttt cccatccctg aatgatgtta tctttggttc
    11161 cagtaattaa atcaccaatc caagatttga atgaagtcca aatgtcttta gcaccttgaa
    11221 ctgtattgtt gaataaatct atcgttcctt gctttaagct gttccaactt tttataagag
    11281 tttgcacgat attattaacc gtttggaaaa accattgttt tagattattc caaagttcaa
    11341 ttgcaccata ttttgtatca atccaagttt gaattatgga gaattttaga tttatccaca
    11401 tttgaattgc actgtattta atatcaatcc ataaattagt aaaaaatagt ttcaaatcaa
    11461 tccaaatcat agtcgcttga taaactactt cattccaaat attagaaata ctttgtatga
    11521 atccagtcca taaactaacg gcactattta ctattgcacc gatatattcc gtaaatatat
    11581 tttttacaga tgtccaaata tttaccactg attggacaag tgtgttccag attagatcta
    11641 aatcctcttt catttgttcg aagtcaccag taattaaatc tataataaac agtagtggcg
    11701 cagctattaa agacttaatg atttcccaag catttactat tatgttttta acctcagcaa
    11761 atatacttgt tattgctttc actatattag agaacacatc tgaaaagcta cttacaaatg
    11821 gaccaatata ttttagtata aaatcaaaac cagatttgat tttacttgta attgttttcc
    11881 atgcagtaga tatagtattt ttaaaccctt cccatttttc accagcactt gaaactaaat
    11941 catcaataac tgatatgaca ccttctttta aactattcca aatctctgaa gcagagtttg
    12001 caatacttga ccataaattt gaaaagaact cttttgttcc agtccatttg tttttaatcc
    12061 aatctgccgc tttcccaggg gcttcttgaa ctgtagtcca aacattgtct gcgccttctt
    12121 taatggactt ccataaattg ttaaaccatt ctcctgtaga tttccatgca ttctgaatcc
    12181 attctactgc cgagcttaca gcagacttga ttccctccca taagccaatc caaaagtttc
    12241 taaaatcttc actcgtattc caaagataga tgaaacctac aacgagtagt gctaccgcag
    12301 ctataaccag aaaaaccgga tttgtaagaa gacccattgc tttacttaat ccactaaaca
    12361 attttgaact ttcacttaaa gctttaaaag ctactttcac cctattgatc catataatta
    12421 ctgaaccaat cataaaaatt aacggtccta tagcagcgac aattgctcct atggccacta
    12481 ctaatttttg agttgattct ggagcactta caaatttttc tactaagccg gatatggcat
    12541 ctgctacttt tttgatggat ggtgctagaa tcttttgaat tacaatagct gctgactcaa
    12601 aagctccaaa catttgctcg atggaagaat tcatattatc ttgcatggtc cgagccatat
    12661 cgtcagctgc accatcagaa tctttcagag attttgttaa tttgcccaat gaatcaggtc
    12721 ctttatcaat caaagccatc atccctgata atgattcttg cccatatagt gttactaaag
    12781 cattttgttg ttgttcaggc gtcaggcctt caaaagcttt tttaagtaat tctacttgag
    12841 tttttaaagg tttcatttta ccgtcagcat cataaaacga aacacctaaa ttatccattg
    12901 tatcttgcat agcctttgtt ggccttgcta acctagacaa tgctcctcgc aacgttgtac
    12961 ctgcttgaga acccttaatg cctgcgtcac tcataatacc aatagctgct gcagtttctt
    13021 ccaaagaaat ccccattgaa ttagctacag gagcaacata cttcaatgct tctcccatgt
    13081 ctccaacttc agcattggta tccgcagcag cacgagcaaa tacatcagcg acatgtcctg
    13141 cttcacttgc ttctaaacca aatcctctca aagcagtagc agtattttca gaagctagag
    13201 ccacatcccc tccagataca gctgctaagt ctaaaagacc cggcattgct ttcatgattt
    13261 cttgtgcgct aaatccagca gaagctaagt tttccattcc agcagctgat tcttttgcgc
    13321 taaaagcagt ttttgctcct agatcaatcg cttgctgttt catctgttcg aatgtgtcgc
    13381 cggttgctcc tgatatagct tttacacgac tcatttgtgc ttcgaagtcg ccaccaactt
    13441 tagcagctgc tacgcctact cctataagag gagtggtaat atactttgtc attgcggcac
    13501 cagtaccttg catcacttta ccaacagcgg ttgtcatact atttgaattc ttttcaaaag
    13561 ttttaacagc atcttgtgca tctttaaaag tctttacaaa tccactatct gtggctttta
    13621 ataaggcttc aacagaaaat tgttccatga ttctcctcct ttcctcaaga gttagcttta
    13681 gttagtaagc tttggaattt tttatcttgt tttgaaagtt cggaaactcc catgattgaa
    13741 tcttcgattt tttgataatt gaagaattct tcaaaggatc gatatacagg aactgtcttt
    13801 ttgcctactt ttttctccgc ttggacttgc tgatttgccc acgctaattc gtgaatcaac
    13861 ttttctttgt caagccaaga taactgggct gcagtcatac gaatgttgta ttcatataac
    13921 gtcattcttt cgatatctga gatattggtc attcccaaat atcgaaaaga attgataaga
    13981 atttgttcgt atgccagtgc agaatctatt ccacttgttg tttttccgct tctttcaatt
    14041 tctgattcag gtttcggacc gctaactttc ccgcgttcga ctccgccaat tcttttagga
    14101 cttcatcaaa caatttttca atgtctttaa cttcatcgat gtaatcatcc aattcatcta
    14161 atgtaataga ttcatcttca gttctatttg ctatttccag aactcgtgat aacgtgttaa
    14221 cgttataaga acgtagctct ggtaagactt ttgcggagag tcccattcca aattccatat
    14281 ttccatcgac gaaaggcatc actttatcta attcacgtac aaatttagtg ccaaatttaa
    14341 acgaatattc tttaccttta atttttaatt tcaatgtttt tcatcctcct aaaataaaaa
    14401 agagagcatc taagccctct tatgctcctg tcgaagttgc tttcacggta tctttgaatg
    14461 catattgaac aacatcggct tgatcttctg tcaaggttgc ataaccatct tgaccaacac
    14521 catttactgc aaatgataaa cttaattcaa cgttatcctc tgcagcagcc gatggagtaa
    14581 attcagacac atatgcttgg taataagtag ctttgtactt atttgcatta tcatctgttc
    14641 cctgttctgc tttattaatt tcccaaattt caatgatatc cccatttaat aaagcttgtt
    14701 tcatttcatc tacatgagaa tctcctttag caactattga agtagccgaa aaatcatatt
    14761 caaccgggct taaactttga acgtttccat cttttgtcac tgtagagtct gaatctcttg
    14821 ataaaccatt ttcgtgttct gtttgaaatg ccattttcca agcggcttcc tgagtttctt
    14881 ttttcaataa gcgataaagc aaaatgacat caataccttt taatgcttcc atgttcttcc
    14941 tcctatctaa ttctaaattc aagtgtgaca accgcccgtt ttagcggtgt attcgttgtt
    15001 gtgtcgtcca tcacttgaat tccacttgct tgataattta aagcccaata atagccttct
    15061 gtggcttcta tcaatctagc ttcattaaaa agagcagatg ccatatctga caactgcttt
    15121 cgtttcttct gtaatcccca aacggataaa accacaatca cagacccttt aatgtcagtt
    15181 ttatttactt catggattgt ctgagtgttc tcaaattcca caaaaggata accaacatcc
    15241 tctaaagttt tgtaatcgta tgttttatat ccaagtttgt tttgggatat tttaaaaagt
    15301 tcatcaaaaa ttgattgatc tcttgtctta atcatcattt caccaaggct ttcatttcag
    15361 ccataaattt gactttttgg taattaaacg ctggtctaac ataaggctgt gccgacataa
    15421 atcgagtgcc atattctaca taaggtgcat aatctgctgt cggtcctaca ataccagtta
    15481 aaccagcttc taaaagattc atgtttattg atcttcgtaa gtaacctgta tccactggcg
    15541 cacctttttg cattcgttca gtcatttcag cagtattact tttcacgact ttttgaacgt
    15601 cattaagcgt tgctgctttt ttcagatgtc gcatcagctg atcgattcct ttatattcaa
    15661 gttgtgcctt catcaagaac cacctcttgc acaattaaac tatttctata tgctggattt
    15721 ctagctgttt tttgttgcca agtctttcct tcaatctcga tatagtcaaa tgtagggata
    15781 gaaaaaagag gctgcgtcct aatgaccttc gccccttctt ccacactacc aaaaatagtc
    15841 acacttctat cagtgccaat atctgtcacg tttgcctctg ttcttgttct ttctggtttg
    15901 ccttcaaccc actcaccgag atctggatca tatttagagt cagatgaacg tttaacaaat
    15961 ataatttcat ctgtaaatct catacaaatt taaacctccc tcgctttggc ttgtacaact
    16021 cttcctgatc tttacgctta aattcatcaa tctcattttg atactctgaa aaatccgagt
    16081 caggaaaagc catagataaa ccttcttgag aatatgactg cataccttct tgaccaattc
    16141 tattgaatct tttcaacgat acttcatata caactgtttc aaattcttta ggaacttctt
    16201 gcgtatttaa caaggttttc atacgctcat tcgttcttcg ctcaataact tcaagctttt
    16261 catctattgt tcctttaaga agttttttaa tatcatctgc aatcatcata tttttacttc
    16321 ctaactagca ggttgacctg tcacattgat tgaagtagtg aattctccag aagtaaatgt
    16381 gaatgttgct gacccttctg ctgcaatcgt tccatcaaaa ccaccatttt catttttggt
    16441 cactgttgcg atagctccat cacttgaagt tgctgtagta gctgcaacaa cagcagctgc
    16501 atcgctagca tctgcaggca cagctgaaat agtaaatgtt ttagtatcgc ctactttacc
    16561 ggtccatgtc ttttgatttg gcacaatacc ggtagcaggc gttacgcttt tggggaaatc
    16621 ttcccaaatg cttcatcttt tacaatcata aatccaacat ccattgttgc acgcaaagca
    16681 atcagttctt gctcaaacaa gttaactggg gttccatctt cattagttaa agtagacaat
    16741 tgggcttctt cagaaatctt aaatgaaata ttatatggga tcccataaaa catgtaatta
    16801 aagtctccag cgtaaagtgt tcctttatct aaagacttaa ggtctactac tggtaatccg
    16861 tcaattgtat tagtagagcg atcataaata aactcaacat ttgacccgac tgtttgagct
    16921 gcagaacgta attctgtacg attttttcgg tttgaaataa acgcattagg ttcgaattca
    16981 ttttctgcta acttgtcttc taaggctagg atattatcat aagtcaatcc gccttcaacc
    17041 acattccccg cactaataac tgatccgtct agtgactgag gaaatgggtt ttctttattt
    17101 aataaggcag ctgcatcaaa ttttttatag aaagcttcag caatttttgg ctgcatctcc
    17161 tcaaagaaat ctgataattt ataatttaaa tattcacgag aaaccggaag aatgacaccg
    17221 agtttttttg cagtcatcgt agcttgcatc catttaggtt tagacgtttt aattttttca
    17281 ccttcaccca cccagtatgc gcctggtcct tctgcaaagt attcaaattt cttttctttg
    17341 tcagtcattt cttcgtattt tgctaactgc atgatcttag aattttccat aacttcactc
    17401 aaaatgagcg tattatattt atcaggaatt tttccctctt tcgtttcata taccaagaca
    17461 ttatctggat cccatgtttg agcaaacatt tgcaagttca ttggtaaaag ttgtttcttt
    17521 ttcattaagt tttcctccta tttgataatt cgatttttag cagctagttt agccactgtt
    17581 tctttagtat ttttcgatgc tgtaaattgt ccaccttcat ttggtggtgt ttgtcttgcg
    17641 ttttctctct taatcaaaga agcaaagtta gtgatgactg ctacagcttg ttttgtggca
    17701 tctgcatcat cagaaacaat cagaccaagt aaatcatcat cgtgtggtaa atttgcatct
    17761 gtcagcattt tagaagcttc tttcgtcatt tcagatagtg cctgtccacg ctttaattca
    17821 gcgatttcag cttctttttg ctccaactca tgctgtagtt tttcttccgc attcattttt
    17881 gccagctttt tagcttcttc tttttttgct gctagttctt tttcccacgc tgcttttgct
    17941 ttattcgtct cagcagcgat cattttcgct acttcatcac gagaaaatgt tttgccagta
    18001 ttgttttctt cttttgcttc gggcggtgtc tcttgtgagc cagctggtag gtttccttgt
    18061 tgtccctcat caccagatcc accatctcct ggttcagaaa aaaattgtaa gttcattggc
    18121 ataaataaac gttttttcat gattaatcct ccacggttac gccgctaccc gataaatttg
    18181 accagttacg ccggtcagcc gaaaatagct ttctctttaa cgcctgtaag ctgtaagaag
    18241 gcacaataaa aagccgttaa tttgtgttaa cggcttgata ttcgttctat ataaggtgct
    18301 gtactgcatc gacaaaatgg atgcatatta ggagcgttac ttcctggctg catatcggca
    18361 acatcaaaaa cttgattatt taacggtata catagtttgc acgccgttgg ttctgctata
    18421 tagatgtact gggtaatgcc tgcatctcta taacttcgtt cttgaatccc tacctgaact
    18481 ctagtcgttt cagtcaccat caaacgttga gtgttgaact tagtgttttc tcgtccttca
    18541 gctgttaaat attctgccaa tttagatgca agttgcttgg gatttttccc catcgttata
    18601 cttctgacta acaacctatc taattctgat ttcaattcag attgatacat ccataagcga
    18661 tcactaaaag acacgtcatc actcaaaaag gagctgttta ttactagttc tatcagcttt
    18721 gcatatccac ttgaagcaat agtcatttct aaaataccgg cttgtctctt caattcagct
    18781 aaaccagctt ttgttaattc attcgaaaag tacttatcca attcattaaa cagtgaaatc
    18841 aattcaagcc cgatattagc ttttaagagc tctaatctat ttacacgcat cgtaagattg
    18901 tatagcttta attcttggtt tgctgtagga gaaaaatctt tctctttgac atacttcttt
    18961 gctttgcgag cgaatgcttt gacatccatt tcactagcac gcttcatcgc ttcgctacga
    19021 gtgattttct ggccattgga gaaactgtcc cactgcgcgt ctatttcttt ttgtatcgca
    19081 tcttgtgcgt attgcatgcg acttttaatt tctgccatgc gctttttatc atctttaatt
    19141 tgttgctttt gccattcctt ttcccgtttg atccaatatt cttgggagtt catttaatca
    19201 cccccctgtt tcatcttttt tgttgttaat tacttcttca ccatctgaat caaaaatgcc
    19261 aatctgcttt tgcgtttctt tatttactcg tttcaactct gcctgtacat ctggaacaaa
    19321 agaagcgagt cctaagatcg tctcttgact gagttcagct ccagcatcaa ccaaagattt
    19381 caactcttcc agaatggctt taggtagatt aggcgtaaat attacacgta agcctttcaa
    19441 atcggagtta tccatttcag aaatacttga ttttaggcta aataaaagac gataacgccg
    19501 cataaggcct tttttgaata gcctttgctt tgttgccgtc atttgttcaa atccaaataa
    19561 tttatatttc attgcttctc ccgattgcac tccggaaaaa ttgtcatcag taagatcagg
    19621 aaccatggat atttcgtgaa tatccttgcg tactctgtat ttgtatgctt ctacaccgtt
    19681 cacatcatat tgtttgtaaa tatatcctgc agtcacactt gttttattac cgttcacatc
    19741 agttccagac tcaagcaaaa gcatattcgc ttctttctgc ttgatggcgt cctctgtgga
    19801 tagtcctgct gcttcaatat caccactaat aactagaaga gcatcgttta gatcagtcat
    19861 ataattggcg gtatcagact gccctgcatc gtatagatca atcaaagata gtacatcttc
    19921 atacaagccc atccgaaaac gattaggaga atactctgta ataggtacct ctttatattc
    19981 atgcggttca tcctggggat tctttaactc aattgctgtt agtgtcgtct catcataagt
    20041 gatacttttt tcttttgtgt atacgattgg ttgaatgtac tgtttatcag catccttgtt
    20101 gaatcttgtc ttaggatacc gtacagccaa aataggctct cgttttactg tagtatcata
    20161 tacaacaaac gtttcaaata cattagccaa atcaacataa tctgtatcat ctgaatctcg
    20221 atagataatc tcataggctc tcccatactt atccatatca agccagagtt cagcatttaa
    20281 cccatctatg tcattattag tattaaactc ttcgatttct ttttgttgat ttgtgtcctc
    20341 gatttgcact tttataggat tgcctgtgtt gtacccaaca tcaaacgtac aaagaacttt
    20401 tccaaagtta tgtgctgatc ggtgatccgc tttttctttt tctctgcgcc tacggttatc
    20461 catgatattt gtatttctag ctttataata atcatctaat acacttagcc tttttacctg
    20521 atattcatga tgatgtttta tcattgctgc taaagtatct aaatcgttaa gtaaatcttc
    20581 tgctgagcta aatctatagt gaagatttga gtctacgcta aactttacat aatttgtgtt
    20641 cacgtcatta gaataatgta tatcagctcc gtattcaaac tcgtttacct tatccatttt
    20701 tcacactcct taaaacatgc gtttaatacg tttaatcttt tcgttgacat taacttttgg
    20761 tttcttaaga agctcatgag tatatatcgc atatcgaacg gagtctagca catcatcata
    20821 ttcttttatt ggctcgcctt ttttcttgtc ccaaatgtat tgataaattt catcccgaaa
    20881 tttcatcact ctatcttgac agataaacaa tttatcttgt ttaaatctct tggcgacttc
    20941 ctcaactcca gataaccgtg ctttatgagc atttttagca ttaatctttt ctcttacaaa
    21001 cctagccaca tgctccggtc tcgcagagtc acaataaaaa ggtactcgta agccgtaacg
    21061 ctcttgaatg ccttttgcca catctaccca ataatcaatc tcttcaaatt gagtagcatg
    21121 ttcttcgatt aaataagctg ttccgtcatc cgtttcacct ataactacga ttgaacccca
    21181 gtgttcatat ccccagtcaa caccacaata aaagttgctc aaaggtggta agtcttttga
    21241 ctggatgtag tgcttgctag catcaaaatc acgataaacg acaccttctg cagaaaccca
    21301 taaacctttg atatcacgat cataaaacat gccgcttggt gttgattctt taatattgtt
    21361 acgatatcgt tcagataaaa atgtattatc atctaattca aagtggaacg attgaatatt
    21421 tttgcttgaa ttgtcgatat actctttctt tagccagtgt tccgggttgt cagggtttgt
    21481 atcagctagt attctcgctc ctgttcctga acaacgtgaa acgatttcag caaatacttc
    21541 ttgtctagct agcgatgcct cattgatata agcgccatat gctgtcatac cacgaattgc
    21601 gccaacaccg ccaatgtttc ctgtataagc ttgtacgacc ttaacgccaa atagcttaaa
    21661 gtttccatgt ttatcgaatt taggttctat gctgtacatg ttgtatagtt cctgtaagat
    21721 gttcttttgg attgttgcac ttgaaactcc tgctaggata tacattggtt ccttaatgtc
    21781 ttcttcatcg gcaatctttc gcacacgtcg caattcaaac aagaacaaat cattgtttat
    21841 ttttgttttt ccagaacgtt ttgctccatg taataaagta ataaaccaat cattcttaat
    21901 ggttttattt aacacgtcga tttgctttgg gttataaata tcaactagtg ccatctaatt
    21961 caccactaat cttttctaac aactcatcaa gtttttcttc ggtagaacgc tctgagttta
    22021 tctgtaattg ttctaatttt gcttgcatta attttatttc tgtttgagct ttttctaatt
    22081 gcgtttgtga taacagcgat ttatacttca aatacaaatc caaggctttc aatttgcttt
    22141 ctaaatctgg tgtatattca tattgcatat gtttaataac cttgtttttc tccaagcggt
    22201 ctatttgctt gctcacgctt atttgtactt ctccttgcca aatatctatc aattcattta
    22261 aagcgtcctc ggcacttaat ttgcgcttct tttcgatagg atttagccgt tcattaatat
    22321 actgtattat gttaggttta gttaggtttt ccttacctat tactcttgct gatcgtttgc
    22381 tataacctgc tttaatggcg gcttgagtag cattaccacc attttttata tattcatccg
    22441 caaaagcttt ttgttttggt gtcaaattta ccacattcaa gccaccacct ttcaatttta
    22501 ttaacaatat ctctcaatat ttgtctgtat gttctgctca ctaaaatatc catggccgca
    22561 gtaacgaaga ttgtacttat caatctcttt tggcgtagct tctctggtca tttcaatgat
    22621 ggagtacttc tttttaatct ggactgactg gacaactcta attggatcat ctgcgtttgg
    22681 ttgcggatat ttatttgata atgatacata ccagtagttc ctcattacgt atcactcttt
    22741 ctgttggtta ctggcagaaa aggttcgcat cataaattcc atagcctgtg cgtcattaaa
    22801 tccttgcaga ataagctgat cgtagaaata cttagcttgt tttgcgatta acgctaagct
    22861 tttttgagtt tcgtagaatg tcgcctccat tgttttgttt gcttcattcg ttttaaacaa
    22921 ctctcttaat tggttttcgt tcattttttt actccccttc caaaataaaa agaccactcg
    22981 atgagtgatc taatatgtaa aaactacacc tcataaacga gatgcagtta tagttctatc
    23041 aagcaaccta caccgattcc atcgattact atcgacctcg ccttgctcgt gtactttgag
    23101 cgcccatttc caaccctcag ttgccaaagt cactggcaag gaatcgaacc ttgcatggtt
    23161 gccgaagcat tgacctaaca cacatgctta gcgtctaccc tttccgccac agtgacaaat
    23221 taatattgtg aaaataaata ctaagtgtat aattttattt atcagcgagt ggtccgctga
    23281 aataaattaa aaggtggtga aaaatttgta ctttgttata agagaagcaa ctaatggcca
    23341 atattacttc gttattaagt ccgataacaa tgaagtagta gcaactagcg aaacatactt
    23401 gactaaatat tccgctgaac aaaccattaa ttctataaaa aacggaatca ccaaagactc
    23461 tcaagttatc gatgtgacta aataagacga ctaacttcat ttgctaggga gttcatttca
    23521 gctgcttttt ctaccaaagc agttgagata ctttctagct tttctgattt aacagaatta
    23581 aatactggcg tcttgatact aatcgataca tcttgacgag gatcaggact cgttgctcta
    23641 tttttttgca ccactgatct gtacttttct actgaatgat actcaattac ttgttctaaa
    23701 tgttttttgt actctttaca tacagcttct ttttggatca attctaaacg ttgttgctca
    23761 ataatatcaa tcaaccgttc ccgatccata ccttgatact ttatttgctc gacttccatt
    23821 cgttttccct ccaatacata aattaataaa cagcaacgga tgatagataa taagaacaat
    23881 ttagaaggag ttggaattca catccttatt cttaatattt ccgttgctgt ctatcgaagc
    23941 ttaattgtga aacaataata aaacgatgtt ccttttatta ttattttgtc tcagacctat
    24001 cactaatctt tcgacactat cataataaat catttaccag gtaagtgatt ggtataaaaa
    24061 aggtataaaa tggaaaccaa atgggtaatc aaagggtata aaaagtgtaa aaactggcta
    24121 cttaaaagca accagttcta acgatgaagc aaattggatg ataattctgt ttgattctac
    24181 tttaaccgat tcttcgctag tattattcct ttgagcagtt acataaatgg gcagaccatt
    24241 gatataacga tcatagaata tcttcttgcg cctttctgtt acatcaggtt tatgcggatg
    24301 ctgaattgct gaatagccac gaacaaataa tttgtgcaag tactcaaatt cttcttgcgc
    24361 ttcctctctc tcaatcatca ttcgttctgc ttcaaatgta ttatttgctg tagaaggtgg
    24421 aaccaaggag taagatgccg ttactttagg ctcccgaggt tgacctatcc tacatctagc
    24481 tgacagatat gctgaaagaa aaacaccaac attatgtttt gtgcgatcca tatccacatc
    24541 ttttgcctct ggtgtctcat acttctttac atcgaaaagt accatccatt gattcctcca
    24601 tttatgatat aataattgtg tcagaattat taattaaggt cggaggaatc cggctttttt
    24661 tatacccatg cttatgctaa gcttttcaag tagcgaggtt gcactcgtta cccatataca
    24721 tgttgagcta tctggcggaa aacagatggc tcactatttc aatattctgc taaggacagc
    24781 cagtggtcgg ctgtcttttt tatattttaa tgagaagcct tacttatttt tttatcttta
    24841 ttacgaaaaa tgatattatc tattgataat aaagcgtata ggagatggtt gagtgactat
    24901 gtggagcatg ctgttatttt ggattcccgt ttgtattggt atcgtcgcat tttgttactt
    24961 tgtcaaacac tctagaacaa ataagctcct catgttatct tttttaccta tagtattttt
    25021 tattgtacaa attgttaaat atacctatat tgaatcgcaa gaaatattca ttttttatgt
    25081 ggtaggttta tttatctctg tggtcttttt cataatgata ctttcctatt tttataaaaa
    25141 ataaattttt ctcttagaag tacatttttt cgctgtttat atagccttca tatccaccaa
    25201 tctcgctaca gctaaattct ctttgctttt cgctaaccac ttatcacatt ccatcgtgtt
    25261 ttcaatacga atgatcgctg agtgattata gacgtgttct acatatccac gaaatggata
    25321 gatgaaccct tctgcttcgc agcgaaccat gtcaccgact ttgaattttg gtttcttacg
    25381 ttttttagga ttctttgtcg gcatatctag cattaaaccg ccgataccat gactactagc
    25441 gtaaaatccg tcttttaatt tcatctttct acctcctcat gatcgacagt gaccagttca
    25501 tatacttgtg ttaatccacc aagtcgtcgt gaaacttggt tagcttcttc gaggctatca
    25561 aaccatctcg cactttggag tatgtctaca atgaataatg tgttagcccc tatagagcgt
    25621 tcatcacttc tataaaattt attcccgaac tttactacat aaatcttcat tctgcttcct
    25681 cctcaatcgt ccaagcattt gtaaatttct ctaagcttat tagttcatct tcatttaaat
    25741 atccgtaacc atataaggat tctaaaaggt cttcttttga atagcctcta aaatcaagtc
    25801 tgagaccgct taggatgtat acataggttt tatttccata aaacttgatt gtgtatgctt
    25861 tcatcccact tcctcctcat cgatctctat ctcgacatct ctgccttcta aaatctgttt
    25921 ggtatcaata taggctacga gtatttttcg attttggctt aaagcaatat acggatcaat
    25981 caaagcaact tctgcgtgta gacgataata agcaatacaa tctatttcta aaaatatcaa
    26041 acgatcaata gcatcattaa tcataaaatc tgtaacgttt tctgctgtat ttaaaccatc
    26101 tgcatattta ggcaggactt cattattgat gaactcagct acatgttttg gacataaatc
    26161 agtcagttct tgttgtgtat agttcatttg ctgtcctcct gattaatata tttcctcaaa
    26221 ctacgtatcc ctttaccacg tttttttgtt ttgcgctcaa tccacgttcc tgtagttccc
    26281 cagtagtcaa taattccatt atgggtatta attgcaaatt gatgattgtt tggataatga
    26341 gtaaattcaa atcccaaggt ttcaatattt ttagttgctg aattgcccat ctttctgaca
    26401 tgttgttttc tacgttcttt tagatgcggt ttaatatctt tccagtattc gcccatatcg
    26461 cccatttcgc tgtcctccta tctccttgtc cacaataaaa accacatcaa ctcttctgtc
    26521 gtaccgttta aataaagatt tatttctgca ccaatcgcaa ctaaaattcc gacaacaaac
    26581 aaaataatcc ctactgctgt atattttttg ccttttgcac tctcgtaaat gaggtatgct
    26641 cctaacatcg ctagtaagcc gccaattaca caccaaaata ttgttaggct gatcatttgc
    26701 tgtcctccaa atcactcgac ttcacgaata caccatctac catttttcct gtgcgtcctt
    26761 tgatttcgtt gtatgcttgg ttcagacact cgtacaaatc catatcattt tgcatagcta
    26821 aaataatcaa ggttactact acatctccaa ttccgtctct taaaccatgt tcatcttttc
    26881 tagctagaga agcggcaact tccccgatct cttcgatcgt ttttaacatt tgcttgctgg
    26941 aatcagcttg atccaatccc ttatctttag cccactgctc tacttttgtg attagttcgt
    27001 ccattattca ttctccttta tatatttaag ttggataata caacttgtaa caaatgaagc
    27061 taatgttaaa attgtgccga ttggtgttaa aaaaccgctt tttatcgcat taattaatat
    27121 agctacaaat atcgttatat agaataaaaa gtgaattgac gctaacgttg aattaaacat
    27181 ctagtcctcc tcgaaatact cattcagtat ctctctatac ttttctacaa atttgaaacg
    27241 atcctgatga agtttcttgc tccaatttgt ttgccgatcc agctcacgca tctgatcgaa
    27301 ccctttttga atttcgttgt aataaaattc aatgtttgct gctgctttcc aatgcctgct
    27361 acttcgcact cctgctcctg tttcagccat ttccaactta actaattccg ctcgttcttt
    27421 tgctttttta tctttctgaa tcttcatcat gattttcttg aggatgatat cactgtattg
    27481 tgtaatgaga tccattattt ctcctccaca tacctaaact gtcgcccctt tgaatcaatc
    27541 cataagctcc tagctctatc ccagatgatg tttttgctca gtccagtaat ttcagataac
    27601 tgttcagctg tacccgttac tagaattcga tcaccatgcc agattgcaat ttttctcggc
    27661 gttttccgtt taggcttttc agtccacatt gatttaccga gcttttggac ttctgcaact
    27721 atttctttgt cttcttgcca attctcagaa tgtgtcagtt caatgattcg tttcattgct
    27781 gctttcttac ccacgctcat tcctccaatc tacgaatttc ccttcttaaa ttcactatgt
    27841 gcaaattgat tgcctttctt gccgtttcat tgaccatcac tgcctttatc cgttccagat
    27901 cgtcaatctc acgttgaagg cttcgaatac gcatttgaat cacttcttct gttgtcatga
    27961 tggaccacct cgttaaaaac gctcttcctt gaacgtattc cgatattttt tagctaatat
    28021 caacggcact tgatattgat gacagaacaa ctttgccttg atcttaaagt cttttgtctg
    28081 cattcctttg acatctacga ctttgacaag tttgccgttt ttataaaatg tgaagtcggg
    28141 aatatactcg atcttgcgat acttttttcc atctagttca aatttcggca tcagctcaaa
    28201 tctttcctga agttttactt tccagccgtt cgcttctgct tgccacaagg ctaaatcgta
    28261 atactctgct tccgcgatag aatcaaactt gatacctcga tgaacagttt tcttattacg
    28321 gtatttattc atgcgatact acctttcact ggttttatgc gcttgtctgc tgtttgttgg
    28381 aatttcagcg cataaccttc tgaattctta aatattctag aaacaattct ttcgccgtag
    28441 gcttctctta gttcaggacc ggataagttt gttgtgatga tcgttgcctt gttctgtctg
    28501 gcttctaaga gcgtgtttaa cgtgttgttt gtaaactgcc tactatttga taccccgcta
    28561 cctaattcag ctccaatatc gtcaaaaacc accaaatcag ttgttttgat atcggctata
    28621 agcgatcctt caatttcttt tctcagttca gcattgttat aagaaaactt tatttgctct
    28681 aataactctt gatagcttat aaaaagtatt ttcttgtcat aatttgagcg ctcaagtatt
    28741 tcccaagctg tcgccattga caagtggctt tttccgcttc ctgatttccc tgatagaatg
    28801 aaatgtgcag gatggttcag taagacatca tttacaaagc ttttagctct ttctaaagca
    28861 attttcgttt cttggtccac tacgtgataa ttctccattt tgcatttaaa caaagtttta
    28921 tctgttaata ccgaaccatt ttgaaaaaaa ctcaacgctc gtgcttttaa gctgtcgtta
    28981 tatatccgtt cggtctgtat atcctctttc acacgtaacg ctttataacc acaactcatg
    29041 catgttggtt tacaacgttc tgaaccatcc ttatttttag ctcgccaact atacaaaggt
    29101 tcgctacatt ctggacattt cccgctttgc actaatactc ttcttattag tttctccata
    29161 gcatttgcta ggctttccat gtgatgcatc tcctttttaa attggcaagt cgtcatattc
    29221 actaggattg ctgtactgta gtttttgact ttgcttttta tgattattct tgtctgcttt
    29281 gatttcgaat ttgagcttct caaatttttc tctcaatttc ttagcacttc taatatttcc
    29341 aaaccaaaat tcatttgtag gtagccaatt gatcacatac tcaatcgctt ctatagacgc
    29401 tttatctctt tcttccatca acctgattgt gtctgcccat ttttcgatat ctactttgtt
    29461 catttctttt ggaaaatctt cagttaaatt actttgcaat tttttagcaa ggcgtaagtg
    29521 ttcgtcagaa tacttacctt tcttttcttc tttatctata tctatatctt tctctatctc
    29581 tatctctaac tctggtgtag ttttgtctgg acatttgtcc gacacttgtc ctccagttat
    29641 taaattccgt tttgcctctt ctattttctt tctgtattct ctttttctat ctgcttcagt
    29701 tgaggatttt ccaatgaaac tttgtatatc agacatataa attgctccgt tatctaatac
    29761 gtcaataagc tgcaaatcac ggaaaatttg taccgctttt tctacgactc ctacagaatg
    29821 tcttgtaata gttgcgagca ttgtagagtt aaatggaatc ctgtcattaa acatcaactt
    29881 accttcgtgt tttagacttc ttaaataaag tttgagaaga atattagaat aaatatagcc
    29941 atctggcata ctttctaaga gaaccatctc gtcactatcg aaaaaattct cttttagttt
    30001 taaatagtag tagcgtttgt tgtcagacaa tattttttac cctcctattc taagtttctt
    30061 aattgtttcc tggtttaact tgatcccttt gatttgatat ttatttttga aattgatcac
    30121 acctatattg tgtttctccg tgtgatgaat cctgcagagt gctgcaaatg tgtactctga
    30181 atgatcaact tctttgcgct ttcgtcttcc tagcgctttg tcaaagtgat cgatgtcagc
    30241 tcctgttttg ccacagatgc agcagactct ttttgtgatg catttgtaga agtaatattc
    30301 ttgattcgct ggtaaaatct catagccttc tttgaaagga atatgatgtt caaagatgaa
    30361 atctaagatg atatttgcta agacattagc atcactcaca gttgtattcg attcgtcttt
    30421 gaggcttatt ttgcgccctg tgacgccttc aaaacggaag taaaagaatt ccttccagaa
    30481 gtccgttggc atgcctgtat cgatgaaaat atcgcctatc agcgcataga tgaagtttcg
    30541 ttgctgcaca gtaaatcgac gtggatcaat aaatcgaact tcaataatcc gatcgccatc
    30601 atatccgtca tacatcgtct ttagtcgatc aatgttcact tcttcattga tcgttgcacc
    30661 tatgtctttt cctttgaact ttttcagaac cgctgaatat gaatcgatca atggtttaaa
    30721 cactcatatc acttctcttt tgtttcttct ctgtactgat cttcaatcca gttaacgcct
    30781 cgttttagaa tgcccaagtc tctcttggtc catttactgt catcagcggt tatagaagcc
    30841 gcatcagtca atgcaacaat tgcttcatca attgattttt cgtacttgtt agcaaccagt
    30901 tgtaaagcat ccaagaatag ctttttgctt ctttgagtag ctggttcaag catcgagaca
    30961 tcttctggca tatcttcgcc agcaaatata tatagcccta gcccaaacat tgctagattt
    31021 tttacaagac agcgcatgat cgttttgttg atatcaaaca tagttgctgc ttcaactcgc
    31081 ttttcgattt ttccaacaat ctcttttttc ttcgtttcgt tattccactg ataatcattg
    31141 acttcgtagg tatatggctc atctttcatt gccttgtttg caccatccat gactggtaac
    31201 cacatgtcac gctttactcc gttgactgtg atactggtaa aaaccatata gcctgttttc
    31261 ttatcaaaga catacggcag atatgtttct ggatcatgat agatttcata atctactgcc
    31321 tcacacactt tgctaacttc tgcccaagcc catgcccacg aaagataagt gaggttattt
    31381 cgtttttcag taacatcttt cacatttatt ttgtataagg tattaaaaag cgtattatca
    31441 cttctatttt tcaaaggttg ttccttttca ctcatcaaat tctgcctcca tttcagcaat
    31501 gtatttctta cctgatccgt aataagagat atcaatcaag ttatctctgt catactcttc
    31561 tagcgcatca atcaagccat cttcgatgac atagatatat tcagtttttt tggacttcct
    31621 cgataaatgg ataagataga catgatccca aatactcaca aaatttccca aatcgtcttg
    31681 atcacatgct agttcttcat ccgtcaaaag attacgtctg atttttcgat tgcttgtttc
    31741 cttgatattc gatttgcccc aactaggatc agtcaaatat tgatctagag tggaaagttc
    31801 ttttttcatg tggtaacatc tccttagatg tattttcttt gtgactcatt gctttggtcg
    31861 gctgagtcac ttttttattt gttgccatgc tttttgctta tcaatatgtt gttggcttag
    31921 gatgtttggt ttattgtgtc tccaccagcg attagcaatt attacgccta tttttagcgc
    31981 ttcagctcta ttcatcctct aacaactcca tttgcttaat aattgtcatc gtcgctgtag
    32041 aaggcatcca attgttaata aaatcgataa catcatcgaa ctgttttgcc ttgatacgat
    32101 ttctagctac tgctccagtg attcgcttaa ttccgccatt caagtcttta aacaattctg
    32161 cttttgcttt gctatttaaa tgctgctgat tacaaataac tctaattttg ttgcgaacca
    32221 tattagaaat tgttcggtaa tctggttcag ctagaagttg attgttcttc aaatcgttta
    32281 aatcagactc gatagcgtct actctttcat tcgtttcttc gtttgcagct agagcaagta
    32341 atgccatttc tcgtttagtt gctggaattg atggcaattg gtcttttaat tctttttcca
    32401 tcttgttgaa ggcctcaata tattgaagtt taaaacttaa agctttctta ccagtgaagc
    32461 ccattgctaa tagagagaag ccgtctctat tcatataaat aattcggtat gattgtttat
    32521 tttgaggatg aacataagta tcttcccaaa ataggtctgc ccaattttcg gcaaccccct
    32581 cttttagttc atcaatagcc gtcaaaacat gtctgtgctt tttatcgaag ctttctgcaa
    32641 cttgtaagct cgtagtcaca gcttctttat ttttcaaaat tactaattct tgcattattt
    32701 cttctctcct ttttgatata attctggtgg ggtgaaaaca tgagcagaaa aattgataat
    32761 ataatattgt taacaactat tttttattta gtaatcggag caactctttc cttactgtca
    32821 tttttgtcca acagtagttt gattgtgtta aatcaaaaaa gcatattaac agaatttgcc
    32881 cacatagatt acttctcttt ttttaaaact ggactaatat gtttatttct atcttcattt
    32941 atcctctata taggaactac attttgtcta atggtgctta atttaaaaat gtttataatt
    33001 gaaaaatgga tagttattat cgcaaagatt ttctcatcga tatcaattat cattccaatg
    33061 acattgacgt tttctaaaga gcaatttaac gtagctacta cattcgtatc ttttatagct
    33121 ctattttcgt ttgctgttcc taaaactttt caaagcaaat ctagcaacgc aaaggttaat
    33181 gtagaaaaca ctgatagaaa aaatgatgaa agttattaaa aaaaataatt ctttcattta
    33241 ttgtcaaacc tttcttatag acattagact tttttagtca gtccctcccg actggctttt
    33301 tcgctctgta ctcagcttca tcaagcccaa tgaaaatcca aagcatgtaa acgatcgtgc
    33361 cgattaatgc ttgtctacta ccccatagac ctaaagcgta gataattagt ggcgcgctga
    33421 atactaatgt tcgattaaac tttcccatgt tgacctcctt taaattttgt atttagccat
    33481 cagttcatcg atgtcctttt tgtcatatct taaaaagcca tctatacgaa tttcttttaa
    33541 accgtgagct attaacttct caaaaccttg atcgtttaca cctcccacat attttctagc
    33601 ttcgatcttt tttaaatatc gtgtttctga caaattcttt tttgaaagtt gactaagtgc
    33661 gtcctttaca acttcaacaa ttgcaattct taaacgagtc aaaaactctt cgcttagaat
    33721 attcatgact tgcctcctat cgaattctgt aatcacggat gatctctaga atagttctgt
    33781 ttgcctttgg tccactccag tgtccatcaa gaatttgttg cattcgtaca cgcgtgtgac
    33841 cataagcagt agccaaatca tccatagtta ttccattctc tttcataaac tctttgatcg
    33901 ccgcacgacc gttatccaag tttgacatta acccacttcc tttttttata aatttgtaag
    33961 ttaaaatgat agaaaaaata taatttccat tgacattttt acactataga gtaaaatgaa
    34021 tacatagaga aataagccta taacaaaacc ttttatttgc acttggtcgc caaactttat
    34081 gctataaggt gtgtttttag tttgcttttt tctatcaaat taacttacgg ataaataata
    34141 actctatatc gtaaaattgt aaagctattt ttactctttt tcgtaaatta ttttttcgtg
    34201 ttggaaagga acgttggaat gacaacattt gaaagaataa aagaactagc gaaaaacaag
    34261 aataaaaacc ttcaacaagt cgctaaagat ttgaagtacg gagagaatta cttttatagt
    34321 ttaaacgcag gaaaacagcc aactgccgaa aagttacaag aaattgctga ctacttcgac
    34381 gtatctgtag attatttgct aggacgtact gagaaaaaaa gatactatga tttaacagaa
    34441 aaagacgagc gtgatatcca aaaagaattg cagtcattaa tcgacgattt atctaatgct
    34501 gatggaatgg ctttttcgaa gaaagataat gaaatgagtg aagcgacacg tgaagctctc
    34561 attatttctt tagagaatgc tttgcgaata gctaaaatag aagctaagaa aaaatacaca
    34621 cctaaaaaat atcgcgatta aaaggagtac gttgtttatg tatttacctc aaattgataa
    34681 caaaattaat aagctggtaa aactatatca aacacgcaat ccttttagaa ttgcacgaga
    34741 attgaagata ctagttttag aagaagattt gggagaaatt tatggttact atagtaaatt
    34801 caaacagata aaaatgatcc acataaattc tagtttagat gagatgggaa aatggataac
    34861 ttgtagtcat gaattaggcc actgtgtttt gcatccgaat gaaaacacac ctatgttgtc
    34921 taaaaaaacg ctagtatctg aattaaaaat tgaaaaagaa gcaaattatt tcgcaactca
    34981 tctgactatt gatccaactt tagacggctt tgatcatatg acaaaatatg aaaagctcgt
    35041 ctgctttgga ttaccagatg aattcgatcg tttcttgtga attataatac aacgtaagga
    35101 ggatatagat gaaaatagga atgagaaaac caagtataaa aaaatccata agcgctagaa
    35161 ccactggaaa agcaaaaaga aaagtaaaaa aagcagttat tcctggttat gggaaaaaag
    35221 gtagcggctg gattaaagac ccaaaaaagg ctgcttacaa taaagtatac aagaaaacta
    35281 cattcagttt ttgggattta tttaaataaa aaaagcccgt gcagcaacac ggactcgaaa
    35341 ccttatttct aagattcccc aataaaaatc atatcataga aatgaggaat aaaaaatgaa
    35401 aaaagtaata acaagtttat tagtttttgc ttctctgtta cttttcactg cttgtggtaa
    35461 aaaagtaact acagaagatt taaaagctaa tgattggttg attgaagcaa ccaaagatga
    35521 cgaacctaat atgatcgtat cattttctga tcacgtcgta tcttttagtg ttgacacaag
    35581 ttctatgaaa tcaacttcaa gtaacgattg ggaggctatg ggagaagaat ttgctaaaag
    35641 cttagtcgac caaatgaatt acaaatttga gtatacactt gaaggcgatg ttatgacttg
    35701 gaaagatgat aaagacaaag ataatgatgc caaatatact gtcagcaaag aagataaaaa
    35761 tattatttta actccagaaa agcctaacaa atctgatgat aatgagaaat tagttttgaa
    35821 accctacaaa aaggaaaaaa actaaagaat ctagaacaac tactagctca acaagtagca
    35881 acgaacaatc atcttcgact acaacagaaa gtactattgc atcttctagt actgtagaac
    35941 aagctgctat tgcgcagaat acgcctcctg aaactagtag tacttactct gctgaaagtg
    36001 tagaaaacta tgcagaagct actcctagtt caagtgttga acaagtttca cccactccca
    36061 gttcaacgga gcaatcagta ccacaagatt atgtaccagc taatcaagct caaccaacgg
    36121 caccttcttc atctgaaacc gaaaccttga ctttgcaacc tggtgaaggg accaaacaaa
    36181 tagctgaacg agctggaatt acggtagatc aactgtttgc attaaacggt atggatccaa
    36241 acaactacat gctttatcca ggacaagaac taagagtgaa ataaaaaaag cacatttgcc
    36301 gcccgaccaa gaacgcaaat gtgcaaagag ctacggtaaa gtaggtctat ttgttgtacc
    36361 ctattttacc atctcgaaat ccgctgtaca accgaacaaa cgtacgaaag gaaagataaa
    36421 gatggcaaaa aaagaaattg ataaacgaat taaaaagcac ttaacaaaaa aaggcgaaga
    36481 aaaatatcag tttagtctgt acttaggagt tgatcctcta acaggaaaaa aaagaaaaac
    36541 aacgagacga ggttttagta cgccattagc cgctgagaga gctttaaaaa gattggaggc
    36601 agatatacag gaaaaaggac tgcaagcttc tacagctcaa aagagcaaga aattcgaata
    36661 tgtttacggc ttatggtttg aaaattataa aaagacagtt aaagaaagca cttggtcctc
    36721 caccaaacaa atttttgaca cacacatctt aaaagtgttt ggagataagt tcattgacaa
    36781 aatagacgtt tttttctgtc aagaagcagt aaacacttgg tccgatagcc atcctaaaat
    36841 ttttaagaaa ataaaaaatt acacatctaa tgtttttgat tacgctgctt ctttacaaat
    36901 tataactagc aatcctatg
//
LOCUS       DAZUKT010000017        36569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000017 DAZUKT010000000
VERSION     DAZUKT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.017"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG816_000880"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG816_000880"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4919791.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG816_000881"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG816_000881"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4919792.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG816_000882"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG816_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4919793.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG816_000883"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG816_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4919794.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG816_000884"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG816_000884"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4919795.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG816_000885"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG816_000885"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4919796.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG816_000886"
     CDS             complement(8053..8523)
                     /locus_tag="ACG816_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4919797.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG816_000887"
     CDS             complement(8569..8994)
                     /locus_tag="ACG816_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4919798.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG816_000888"
     CDS             complement(9061..9330)
                     /locus_tag="ACG816_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4919799.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG816_000889"
     CDS             complement(9341..10936)
                     /locus_tag="ACG816_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4919800.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG816_000890"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG816_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4919801.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG816_000891"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG816_000891"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4919802.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG816_000892"
     CDS             15402..16376
                     /locus_tag="ACG816_000892"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4919803.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG816_000893"
     CDS             16546..17946
                     /locus_tag="ACG816_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4919804.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG816_000894"
     CDS             complement(18024..18746)
                     /locus_tag="ACG816_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4919805.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG816_000895"
     CDS             complement(18825..19331)
                     /locus_tag="ACG816_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4919806.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG816_000896"
     CDS             19454..21253
                     /locus_tag="ACG816_000896"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4919807.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG816_000897"
     CDS             complement(21321..22895)
                     /locus_tag="ACG816_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4919808.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG816_000898"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG816_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4919809.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG816_000899"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG816_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4919810.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG816_000900"
     CDS             complement(24672..25310)
                     /locus_tag="ACG816_000900"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4919811.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG816_000901"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG816_000901"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4919812.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG816_000902"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG816_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4919813.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG816_000903"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG816_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4919814.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG816_000904"
     CDS             complement(29104..29844)
                     /locus_tag="ACG816_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4919815.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG816_000905"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG816_000905"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4919816.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG816_000906"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG816_000906"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4919817.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG816_000907"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG816_000907"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4919818.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG816_000908"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG816_000908"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4919819.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG816_000909"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG816_000909"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4919820.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG816_000910"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG816_000910"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4919821.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6705 g        11990 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gttcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatac
//
LOCUS       DAZUKT010000018        35882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000018 DAZUKT010000000
VERSION     DAZUKT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.018"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG816_000911"
     CDS             <1..1152
                     /locus_tag="ACG816_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFS4919822.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG816_000912"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG816_000912"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4919823.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG816_000913"
     CDS             2728..3003
                     /locus_tag="ACG816_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4919824.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG816_000914"
     CDS             3009..3776
                     /locus_tag="ACG816_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4919825.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG816_000915"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG816_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4919826.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG816_000916"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG816_000916"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS4919827.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG816_000917"
     CDS             7920..8222
                     /locus_tag="ACG816_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4919828.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG816_000918"
     CDS             8225..9388
                     /locus_tag="ACG816_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4919829.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG816_000919"
     CDS             9414..12842
                     /locus_tag="ACG816_000919"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4919830.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG816_000920"
     CDS             12863..13987
                     /locus_tag="ACG816_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4919831.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG816_000921"
     CDS             13968..14414
                     /locus_tag="ACG816_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4919832.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG816_000922"
     CDS             14398..14736
                     /locus_tag="ACG816_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4919833.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG816_000923"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG816_000923"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4919834.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG816_000924"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG816_000924"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4919835.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG816_000925"
     CDS             15893..16936
                     /locus_tag="ACG816_000925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4919836.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG816_000926"
     CDS             17095..17823
                     /locus_tag="ACG816_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4919837.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG816_000927"
     CDS             17939..18184
                     /locus_tag="ACG816_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4919838.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG816_000928"
     CDS             18181..18399
                     /locus_tag="ACG816_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4919839.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG816_000929"
     CDS             18535..19074
                     /locus_tag="ACG816_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4919840.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG816_000930"
     CDS             19067..21340
                     /locus_tag="ACG816_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4919841.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG816_000931"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG816_000931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4919842.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG816_000932"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG816_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4919843.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG816_000933"
     CDS             complement(23167..23436)
                     /locus_tag="ACG816_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919844.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG816_000934"
     CDS             complement(23698..23826)
                     /locus_tag="ACG816_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919845.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG816_000935"
     CDS             complement(23906..24361)
                     /locus_tag="ACG816_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4919846.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG816_000936"
     CDS             complement(24499..26427)
                     /locus_tag="ACG816_000936"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4919847.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACG816_000937"
     CDS             complement(26624..27457)
                     /locus_tag="ACG816_000937"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4919848.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACG816_000938"
     CDS             complement(27649..28353)
                     /locus_tag="ACG816_000938"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4919849.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACG816_000939"
     CDS             complement(28376..29002)
                     /locus_tag="ACG816_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4919850.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACG816_000940"
     CDS             29328..31703
                     /locus_tag="ACG816_000940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4919851.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACG816_000941"
     CDS             complement(31727..32218)
                     /locus_tag="ACG816_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4919852.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACG816_000942"
     CDS             32405..33325
                     /locus_tag="ACG816_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4919853.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACG816_000943"
     CDS             33479..33823
                     /locus_tag="ACG816_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4919854.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACG816_000944"
     CDS             complement(34013..34513)
                     /locus_tag="ACG816_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4919855.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACG816_000945"
     tRNA            35041..35124
                     /locus_tag="ACG816_000945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="ACG816_000946"
     CDS             complement(35448..>35882)
                     /locus_tag="ACG816_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4919856.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7405 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAZUKT010000019        35847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000019 DAZUKT010000000
VERSION     DAZUKT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.019"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..96)
                     /locus_tag="ACG816_000947"
     CDS             complement(<1..96)
                     /locus_tag="ACG816_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HFS4919857.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNY"
     gene            complement(102..1361)
                     /locus_tag="ACG816_000948"
     CDS             complement(102..1361)
                     /locus_tag="ACG816_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4919858.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1363..2409)
                     /locus_tag="ACG816_000949"
     CDS             complement(1363..2409)
                     /locus_tag="ACG816_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919859.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(2644..2919)
                     /locus_tag="ACG816_000950"
     CDS             complement(2644..2919)
                     /locus_tag="ACG816_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4919860.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3162..4472)
                     /gene="der"
                     /locus_tag="ACG816_000951"
     CDS             complement(3162..4472)
                     /gene="der"
                     /locus_tag="ACG816_000951"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4919861.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(4654..5883)
                     /gene="rpsA"
                     /locus_tag="ACG816_000952"
     CDS             complement(4654..5883)
                     /gene="rpsA"
                     /locus_tag="ACG816_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4919862.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6018..6698)
                     /gene="cmk"
                     /locus_tag="ACG816_000953"
     CDS             complement(6018..6698)
                     /gene="cmk"
                     /locus_tag="ACG816_000953"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4919863.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(6747..7382)
                     /locus_tag="ACG816_000954"
     CDS             complement(6747..7382)
                     /locus_tag="ACG816_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4919864.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7448..8851)
                     /locus_tag="ACG816_000955"
     CDS             complement(7448..8851)
                     /locus_tag="ACG816_000955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4919865.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(8838..9869)
                     /locus_tag="ACG816_000956"
     CDS             complement(8838..9869)
                     /locus_tag="ACG816_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4919866.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            9900..10121
                     /locus_tag="ACG816_000957"
     CDS             9900..10121
                     /locus_tag="ACG816_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4919867.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10286..11023)
                     /locus_tag="ACG816_000958"
     CDS             complement(10286..11023)
                     /locus_tag="ACG816_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS4919868.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11176..12516)
                     /locus_tag="ACG816_000959"
     CDS             complement(11176..12516)
                     /locus_tag="ACG816_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4919869.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(12753..14276)
                     /locus_tag="ACG816_000960"
     CDS             complement(12753..14276)
                     /locus_tag="ACG816_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4919870.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(14654..15265)
                     /locus_tag="ACG816_000961"
     CDS             complement(14654..15265)
                     /locus_tag="ACG816_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4919871.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15388..15513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(15595..16311)
                     /locus_tag="ACG816_000962"
     CDS             complement(15595..16311)
                     /locus_tag="ACG816_000962"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4919872.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16317..16886)
                     /gene="scpB"
                     /locus_tag="ACG816_000963"
     CDS             complement(16317..16886)
                     /gene="scpB"
                     /locus_tag="ACG816_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4919873.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(16870..17640)
                     /locus_tag="ACG816_000964"
     CDS             complement(16870..17640)
                     /locus_tag="ACG816_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4919874.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(17637..18008)
                     /locus_tag="ACG816_000965"
     CDS             complement(17637..18008)
                     /locus_tag="ACG816_000965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4919875.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18027..18914)
                     /gene="xerD"
                     /locus_tag="ACG816_000966"
     CDS             complement(18027..18914)
                     /gene="xerD"
                     /locus_tag="ACG816_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4919876.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19010..19483)
                     /locus_tag="ACG816_000967"
     CDS             complement(19010..19483)
                     /locus_tag="ACG816_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4919877.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(19588..20451)
                     /locus_tag="ACG816_000968"
     CDS             complement(19588..20451)
                     /locus_tag="ACG816_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4919878.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(20647..22746)
                     /locus_tag="ACG816_000969"
     CDS             complement(20647..22746)
                     /locus_tag="ACG816_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4919879.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(22966..24075)
                     /gene="rpoD"
                     /locus_tag="ACG816_000970"
     CDS             complement(22966..24075)
                     /gene="rpoD"
                     /locus_tag="ACG816_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4919880.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(24099..25940)
                     /gene="dnaG"
                     /locus_tag="ACG816_000971"
     CDS             complement(24099..25940)
                     /gene="dnaG"
                     /locus_tag="ACG816_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4919881.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(26122..28437)
                     /locus_tag="ACG816_000972"
     CDS             complement(26122..28437)
                     /locus_tag="ACG816_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4919882.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(28622..29482)
                     /locus_tag="ACG816_000973"
     CDS             complement(28622..29482)
                     /locus_tag="ACG816_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4919883.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(29541..30173)
                     /locus_tag="ACG816_000974"
                     /pseudo
     CDS             complement(29541..30173)
                     /locus_tag="ACG816_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            30340..31101
                     /locus_tag="ACG816_000975"
     CDS             30340..31101
                     /locus_tag="ACG816_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4919884.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31127..33718
                     /gene="mprF"
                     /locus_tag="ACG816_000976"
     CDS             31127..33718
                     /gene="mprF"
                     /locus_tag="ACG816_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4919885.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(33906..35093)
                     /gene="tuf"
                     /locus_tag="ACG816_000977"
     CDS             complement(33906..35093)
                     /gene="tuf"
                     /locus_tag="ACG816_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4919886.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            35461..35817
                     /locus_tag="ACG816_000978"
     CDS             35461..35817
                     /locus_tag="ACG816_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4919887.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         9972 a         7581 c         6157 g        12137 t
ORIGIN      
        1 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
       61 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
      121 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
      181 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
      241 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
      301 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
      361 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
      421 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
      481 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
      541 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
      601 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
      661 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
      721 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
      781 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
      841 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
      901 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
      961 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     1021 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     1081 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     1141 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     1201 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     1261 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     1321 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     1381 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     1441 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     1501 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     1561 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     1621 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     1681 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     1741 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     1801 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     1861 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     1921 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     1981 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     2041 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     2101 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     2161 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     2221 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     2281 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     2341 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     2401 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     2461 gaaaagataa gagcaaagct cacctcataa cttgcaaata aataaaaatt aaataaaata
     2521 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     2581 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     2641 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     2701 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     2761 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     2821 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     2881 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     2941 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     3001 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     3061 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     3121 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     3181 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     3241 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     3301 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     3361 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     3421 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     3481 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     3541 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     3601 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     3661 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     3721 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     3781 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     3841 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     3901 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     3961 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     4021 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     4081 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     4141 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     4201 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     4261 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     4321 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     4381 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     4441 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     4501 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     4561 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     4621 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     4681 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     4741 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     4801 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     4861 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     4921 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     4981 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     5041 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     5101 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     5161 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     5221 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     5281 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     5341 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     5401 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     5461 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     5521 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     5581 acggcgtttt gaaagtagat agctgccgtt ttctttatct ttgccgatcg tactgataac
     5641 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     5701 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     5761 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     5821 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     5881 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     5941 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     6001 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
     6061 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
     6121 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
     6181 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
     6241 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
     6301 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
     6361 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
     6421 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
     6481 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
     6541 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
     6601 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
     6661 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
     6721 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
     6781 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
     6841 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
     6901 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
     6961 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
     7021 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
     7081 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
     7141 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
     7201 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
     7261 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
     7321 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
     7381 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
     7441 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt ccccaatgaa
     7501 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
     7561 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
     7621 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
     7681 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
     7741 gctccatcca ttttttttgc agctcatcaa agggagcttg ctctgtggca tattccaaat
     7801 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
     7861 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
     7921 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
     7981 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
     8041 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
     8101 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
     8161 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
     8221 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
     8281 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
     8341 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
     8401 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
     8461 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
     8521 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
     8581 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
     8641 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
     8701 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
     8761 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
     8821 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
     8881 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
     8941 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
     9001 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
     9061 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
     9121 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
     9181 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
     9241 ctttgttgca gataaaccgc tgactcatca ttgttttttg ccagctgata aggaagcagc
     9301 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
     9361 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
     9421 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
     9481 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
     9541 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
     9601 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
     9661 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
     9721 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
     9781 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
     9841 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
     9901 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
     9961 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    10021 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    10081 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    10141 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    10201 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    10261 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    10321 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    10381 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    10441 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    10501 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    10561 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    10621 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    10681 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    10741 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    10801 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    10861 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    10921 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    10981 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    11041 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    11101 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    11161 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    11221 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    11281 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    11341 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    11401 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    11461 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    11521 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    11581 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    11641 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    11701 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    11761 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    11821 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    11881 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    11941 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    12001 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    12061 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    12121 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    12181 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    12241 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    12301 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    12361 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    12421 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    12481 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    12541 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    12601 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    12661 atggagctat tgacttatcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    12721 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    12781 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    12841 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    12901 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    12961 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    13021 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    13081 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    13141 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    13201 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    13261 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    13321 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    13381 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    13441 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    13501 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    13561 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    13621 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    13681 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    13741 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    13801 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    13861 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    13921 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    13981 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    14041 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    14101 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    14161 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    14221 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    14281 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    14341 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    14401 tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg
    14461 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    14521 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    14581 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    14641 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    14701 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    14761 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    14821 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    14881 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    14941 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    15001 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    15061 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    15121 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    15181 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    15241 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    15301 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    15361 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    15421 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    15481 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    15541 ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa gtgttcattt
    15601 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    15661 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    15721 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    15781 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    15841 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    15901 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    15961 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    16021 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    16081 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    16141 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    16201 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    16261 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    16321 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    16381 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    16441 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    16501 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    16561 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    16621 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    16681 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    16741 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    16801 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    16861 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    16921 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    16981 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    17041 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    17101 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    17161 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    17221 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    17281 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    17341 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtga cgaattcttg
    17401 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    17461 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    17521 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    17581 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    17641 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    17701 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    17761 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    17821 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    17881 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    17941 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    18001 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    18061 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    18121 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    18181 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    18241 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    18301 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    18361 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    18421 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    18481 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    18541 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    18601 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    18661 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    18721 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    18781 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    18841 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    18901 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    18961 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    19021 ggtttctttt ttctttttca tagcattcct gacagattcc atggaacgtt agccgatgat
    19081 cttttactaa aaaatgataa cgttcttcta ctacttgctc cacttcaagt aaaagatcct
    19141 cttctacttc ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg aaatgtttgg
    19201 cgccttcttt tctcaaatca taacgggcta atccatcatt aaaacttact ttatcgacaa
    19261 ttttcaattc tgtcaagatt tcaagggttc gataaacagt agcaagtcca atttccggac
    19321 tctttttttt cactaaaaag aagatttctt ctgcagataa atgctctttc tcattttcca
    19381 aaagcacaag cagagtagct tctctttgcg gtgtcagctt aaaccccgct tcatgtagct
    19441 gctgttttgt ttttttcatc gccgaaatag cattcatgac catttactca accccaacaa
    19501 tttataataa ttacaaataa aaaaagatat aacactatgt taattaaaat tattataagc
    19561 tagttaagaa aaacatgcaa gggattctca attagatttt ttgactagtt ttgtcacacc
    19621 gttttcttgt gtaatcaatc cttgtttcaa aagattacct aatgcgcgct taaatgctcc
    19681 tttgctgata ccaaaagctt tcatgatttc ttctggatcc gatttatctg taaagttgat
    19741 ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat ctgatatagc
    19801 ttcatatgct cttggcttca atgaaagatt cagtacaccg tctggtcgga caccaatcac
    19861 tcgacctttt atctgctcgc ctagacgagg ttcattatat cgttcagaag gatggataaa
    19921 cccaagatag aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc ggtatgctgt
    19981 tcctatcaca tctttgtttt tcatttcttc tgttccatat ttgcttaaag atttgaatat
    20041 tttttcatct gctaaactgc cccaaatacg gtcttttgca tcaactctaa gggaaatcat
    20101 caagcgatcc ccttttttag gccatagctc tgtcattgtc ggcaattcat caagagatac
    20161 gacaaagtct ttatctggaa gaccgatatc gacaaacgca cctaaatctt ttcgtgtagc
    20221 agtaacgatt ccaaaagcgt aatgaccgat acggctttta ggaatttgtg tagtgatccg
    20281 attttcatgt ttttgatttt ggtaaccgaa tccttcaact gcttcaccga ttttgtgttc
    20341 accttctgat ttgcttaagc caaaggtttg tccgtttttt tggacaaagt agtttttttc
    20401 attttcatca ataataatac ctgtaaatat agttgctaat aaatcgttca tttttttctc
    20461 cctctatagt gaaaggtttt cttaatacat ttctttgcta tcataacata atgttatcta
    20521 cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa cagttgtgtc
    20581 atactcccct ttttcgaata aacggtgtta aacggcaggc tcacagcctc tttcatttgc
    20641 ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt ttacagttga tggttacgat
    20701 gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat gcagattttg ttgaagtggc
    20761 atatagatta taatagcctt ccccattttc gattctggct ttttctaatc tgtaacctcc
    20821 ttcaacgata tatccacgtt ggatacatgt agcaatcact ttgctttgga cacctggtgc
    20881 ccacgtccat gctggattag atgcatcggc aatatcactt gctctggcag atttgattgg
    20941 ctgattcgct gagtttgaag aggaaggggt cgaggatgcc gcttcttgct gtgctgcttg
    21001 tgcgaccgca gcttgttgag cggcttgagc tgcagctgct gcttgagcgg cttgttttgc
    21061 ttgttcttca gctgcttttt tcgcttcttc tgcttttttc ttttcctctt cttctttttt
    21121 ctctttatca gtgagcatct tatctacttg tttcaattgt tcattcaact gagcagatag
    21181 atctgtattt ttcacttttt ctactgcttc ttttgccgtc ttgtattggt cgcgtgttgc
    21241 tgaatccact acttttccgt ctttaaagac tatagctgtt ttttctttag ctgtttgcaa
    21301 ttcagcatat tgatttttag cttctgtttg tgcttgttga atcaattttc caaaatctgt
    21361 gttatccgtt tcgattgctt ggatctcttt atctgctttc aacttgatgt catctgctag
    21421 atgatcatcg acgatggcag gtgctatgaa ccactcattc actttttgga tatctcggat
    21481 cttagattgg atatcttggt aagttgtttc taactctgaa tatccttttt cattcttaag
    21541 actggataaa tcatttttta gttttgataa atcttgatga atcatatctg cacgaataaa
    21601 ttgatggtga tcatctgtat aaaaggcagc aagtttctca tgaagttctt ccatttgttg
    21661 ctgtcttttt gcttctgctg ctacttcggc ttctactttt tgatgatggt cataataagc
    21721 ccagcttcct ccaccgagta gaagtactgc tgctaaaacg agataaggta ctttttttga
    21781 ttttttcttt tgcgctttat ctggatctgt tttttctaag tttgattctt gatgtgtttt
    21841 ttcagtatct ggcacgattg ttattgattt cgcttctgct tcaaacagct taggctggtt
    21901 ctcttctttt tctatttttg cttcaagatc agtaccctct ttgcttacag attgattttc
    21961 agcatcggat tcactattta tttcatcggg ctctgttgac tttttgtctg tgttttcgag
    22021 tatttctttt ttctctgctt ccggcagttt attcgttttt tccttttcta attgtttttc
    22081 agtaaatact tcagtttttt ctgtgatatc cgtttttacg tcttttattt ttactgtttc
    22141 tttacctgtg gcgttttctc tatcagagtc tttttcagtt tcttgttcct tttctggctc
    22201 tactttgttt tcctgtgcaa ttttttcact gatcattttg agcagctctt tcgcgtgctc
    22261 ttcttttgta tcttcacgat gtgctttgat ataagcggat agtatcgaat tttcttcctc
    22321 attatcgacg atttctttca gttcagctac tcttcttttt tgttttgatt caagcgtttc
    22381 ttcttgattc tttcctgatt cttccgaagt ctcatgaaca gttgctttct tctgttcatt
    22441 tttctctata ttagttactt gatcgtgagc aggtggttct tcgtcttctg gttctgcttt
    22501 ttcgaataat tccattacag attctagagg taaatctttc aattccgacc attcgattgg
    22561 atcgtttaat tctgtatcag gaaagactgc atcttcagtt gatttatcct gcatagctgc
    22621 atctgtattt gtttcttcaa ttgattcttt tatttgctga tcttctgcag ttatttgatg
    22681 accgcagtct ggacagatag tattctgctc catttttttc gatccgcaat ttggacattt
    22741 tttcatcact tagcgatcgg ctcctttact aggtttacgc taccatttaa tctatcatat
    22801 tctatacgtc acgccaatag cttattttta ggatttactt aaaaataatg aatgaatttc
    22861 cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa gctttttttc ttttaaaata
    22921 aaccaaacag ttttactgat ttctcttctg gtcaatagtc cacgcttatt ctaagaaatc
    22981 ttttaattgt tttgaacgag aaggatggcg caattttctt aatgctttag cctcgatctg
    23041 acgaatacgt tcccgagtaa caccaaatac tttccctact tcttccaatg ttctcgtccg
    23101 gccatcatct aaaccaaaac gtaagcgaag gacattctct tcccgatcag tcaatgtatc
    23161 tagtacatct tccaattgtt cttttagtaa ctcataagct gcgtgttctg ctggacttgt
    23221 tgcttcctgg tcttctatga aatcacctaa gtgagagtca tcttcttcac cgattggcgt
    23281 ttcaagggaa acaggttctt gcgcgatttt caagatttct cggacttttt ctgttggaag
    23341 atccatttcc gcaccgattt cttctggcgt aggttctctt ccaaggtctt gcagcaattg
    23401 acgttggata cgtatcaatt tattaattgt ttcaaccata tgaactggaa tacggatcgt
    23461 acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga atccaccatg tagcataggt
    23521 tgaaaactta aatcctttgc ggtagtcaaa tttttcaact gccttcatca gtcccatgtt
    23581 tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct acataacgtt tagcgatact
    23641 tactactaaa cgaaggtttg cttctgccag acgctgtttc gcttcttggt cgccttcttc
    23701 gattttcaat gctaacgcta cttcttcttc cgcagttaaa agggagacac ggccaatttc
    23761 tttcagatac atacgaaccg ggtcattgat ttttacaccg gttggcgcag aaagatcttc
    23821 tgtttttgct tgttcagctt ttttctcagc actcttcaag ctgtgaattg atggatcacc
    23881 attttcatcc accacactga tacctgcgtc ttcaatcttt tggatcaatt tgtccatttc
    23941 atctgcattc aaagtgaatg gtgtagcaag ctgattggat aaatcatcat atacaacttg
    24001 tcgtttgggt ttgttttctt tgatgaaagc tgctactgct gcttcatacg tttttttatc
    24061 tgttccattt gccataaaag aaggcccccc ttgagtagct atttggcttg ttttaactgt
    24121 ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa gctgtttgtt tccagttcgt
    24181 ctggcttctt gctgcatttg tttttttaat gttatttcat cagctaagcc agatttgctg
    24241 atcacatcta gcaagtcttg gatttctcgt ggcgaacttt cttctgccac cggtaaatat
    24301 tcgatttcca ccaccaaccg ccttaaattt tcatcttgca aaaagtctaa aaactctgca
    24361 aggttgaatt cataatgttg tgagacatat gcatcgaata aaaaataaac ttctgcatat
    24421 gcatcatgaa taaactgaaa ttctcgttct tttaaaatat tacggacaga aacttcttta
    24481 aatgcacgat ataatagaat ctgctctgcc ttttctactt gcgtcaaagg tctctttttc
    24541 cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt cctgaggttc ttgacgattt
    24601 tgctgatttg tccttcgaag atcgcgcagt tgtttttgta tcgtctctct gccgatctga
    24661 aactctgttg acagttgaga tagatacata tcttgttcga ttggtgaaac aactcggcta
    24721 agttcaacta gtaattcttc gaggtattcg atcttgtctt tttcattatc taaatttttc
    24781 ccttgtttta aataacgtga tttaaaagta aagactgttt cgcgcccatg agccactaat
    24841 tcttggaaag atgcttctcc atacttacgg agatattcat caggatcaag cttttctgga
    24901 acgactacga cagaaagtgc taatcgactg ttttgccgaa gcaattcaat ccctcgattg
    24961 gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga ccaattcttt cgtcagcttt
    25021 tccatttgtt ggatctgttg agctgttaga cttgtcccca tagaagctaa tccagttttg
    25081 ataccagcct gccatgcagc aatcacatcc ataaaccctt cgaacaacaa aatttctccg
    25141 tttttccgga tatcgcttct tgctttatca agattaaata aaacttgtct tttattgaac
    25201 agttctgttt ctggactatt caagtatttg ggctgatctt tttcctgccc tttttcaggt
    25261 gcaagccagc gtcctgaaaa accgatcgtc tggccttgag aattacgaag tggaaacatc
    25321 actctttgat aaaaacgatc agacatacgg ccatcatcat gcgtgacaaa caatccagag
    25381 cgttctaaca attcatttga aattgattca ttcttgaata cttgctgaag aaaagaacgc
    25441 tcattaggtg caaaacctat gttgaattct tcaattaatt ctaaagacaa cccacgactt
    25501 tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca atagcatgtg atgatagatt
    25561 tccgctgctt tttcatgagc agcaatcagt ttccccgtag cagattcagg attttggaca
    25621 gctatagtac gctgacgcca ttgatcttct aacggtatct gttcgaagtc tgccactttt
    25681 accactgctt ctggaaaact tagaccttct agttcttgaa gaaaagtaaa cacgtttccg
    25741 cctttaccac aaccaaagca atgaaaaatc tgcttgtctt ctgcaacaga aaaagacggt
    25801 gtacgttctt catgaaaagg acacagcccc aagtaatttt taccagactt tttcaactgg
    25861 acatattgtc caatcacctc aacgatattc gtttgactgc gtactgtttc gataacctct
    25921 tgaggaatcc gttgagccat tttttcaccc cctgctctat tgatatcttt tgggtttaac
    25981 agaagatatt tcaggagaat aaccggtaaa aaatcgcact aactgaaatc agtgcgattt
    26041 taaaggacac aattatacat gtttgattgt atcatatttc attgcttttt caagtataac
    26101 acatttctat ctcattaagc atcaatttgc tactcttttc agtaactgat tttgaatatc
    26161 agccttccag tacaatactc gaagaacaat atttacgagc atgaatacaa agaagtatcc
    26221 tacgctctct cccaacccta agtttacttc caaaattcca tggaacagca ttgcagctac
    26281 atatatccct acgatcgtcg ttactgctag gaagatgcgc aatgggttga agggataaca
    26341 agcttttaca actgccatgc aactgatccc aatcagcaag taatacatca aagtagtagt
    26401 ttcagcattt ccccatgcga atgtttttcc aagtagatac accgcaatga tattcactac
    26461 tacaagcaag gcatttggta aagcattaat aagtgctgta ggcaaataac ggtaagtgat
    26521 ttttctttta tcttgttcaa aagataaaaa gaaggaagga tacccttcaa tcgctaaatc
    26581 aatcagcgtg atttggatag ggataaacgg aaatccaatt gctgtaacgg cacatataat
    26641 cgataaaata aatgaataaa tcgttttgat aaaaaagatg cctgagactt tcgtcacatt
    26701 gttgaccact cggcgtcctt caaacaggac ttctggcaat gtagtaaaat cagagtccaa
    26761 caataccaga ttcgcaatct gacgtgttgc tccatcacct tctgccatag caatgctgca
    26821 atctgcttct cttaacgcca aaacatcatt cacaccgtcg cctgtcattg caaccgttct
    26881 gccgctttct ttcaattcat ttaccagcaa tttcttttgc tgcggactca ctcgtccaaa
    26941 aaccgtatag cgatgagcag cttctcgcac ttccatttct tcagtaattt gagaaagatc
    27001 gacatacgca tcataattcg gtaatcctgc acgtctagcg atattggaga cagtaaaagg
    27061 attgtctcca gaaatcactt tcagatccac accttcctca tgaagataag ctagtgtttc
    27121 ttgagcgttt tttcggattg gatcatcgat ttcaaacaga gcaaccggct caacaaatgc
    27181 tggtttttct tcgttcaatt gctgatttgg agcgatcccc aacataagta cgcgatagcc
    27241 attcgcttgg gctgttttta gactgtccgg aagccgatct tcttccacca aacgttcagg
    27301 cgctcctacg tacacgactc ccagttctgg aaattccatc gcgccccatt tgcgatcaga
    27361 agaaaatgcg actttgtttg tcacatcata ccgattagaa agctcaaaat aatctctcat
    27421 agcccgcata gtgatgttgt tatcactgct ttctgccaga tacgtcccca taatttctgg
    27481 gatggctttc tcgtacagcg gatgtagtgc ttctacccgc tggatactca ttttcccttc
    27541 agtaatggtc cctgttttat ccaagcataa tgtatcgaca tgggccagcg tttcaattga
    27601 atacatgtct tgaacaagga ttcgtttttt tgccagtttg gttacccctg tcgttaatgc
    27661 aatacttatt agcaatacca aaccctttgg aagcatgcct aacaaagctg ctgcagatgc
    27721 aataacagaa attttgatag aagcatcgcg gatgaacagt gcttccaaaa atagtatgat
    27781 ccccaaagga ataatcacga aactagtgaa tttggatact ttgcgaatcg aatctacaag
    27841 ttcagaacga atagctttgt gagtctttgc ctcgctagtc aagcggacag cataattatc
    27901 tgcacctact cggataactt cagccatgac agaaccgctt gtcaaaaagc ttccagaaag
    27961 caattcagct ccagtttctt tgacaatcaa atcagattcg cctgtcaaaa gagcttcatt
    28021 tgcttctgcc cgtccagata taacctgtaa atcggctggt atctgttctc ctgctccgat
    28081 ttttaccaaa tctcctaata ccagctcttt agtagaaatc gattgttctt taccgtcccg
    28141 gatcactagt acttctgatt gggaaacaat cgataatttc cgtacaagat ttcttgcatg
    28201 tatttcttga taaattccaa taacgatatt gacgacaata attgctaaat acgccatgtt
    28261 ggaatacgca cctacaaata acagacaaat cccaatacca aagttcagaa aattgaataa
    28321 cgtcaatata ttatcgctga atatttgttt tgtacttttt gatgcatctt cctggtagtc
    28381 attttgctgg ccagaagcca ttttctgaac tacctcttgc tgtgtaagtc cattcatttt
    28441 ctttttctcc attttccgcc aactttcaaa aataatcacg tgtttaagtc tagcgtgttc
    28501 caagattaaa aacaacttgt ttttataaaa tttatagaaa aacacacgaa tgattttaca
    28561 aaaaagatgc caaccttttt gtttgaatca cccgtgtgct gtttttatta tcaatctatc
    28621 tttacttata ttgttcctcc aaacggctca tccaccaacc tagtgctgta ccggctataa
    28681 aaagaacagg acaagctagt accccagcaa ctcccaatcc cgcgtaatcg gtaggaatga
    28741 tcataatcgt tgaagcaaaa actacaccta aaatgaaatg gaatagcttg ctatatgcct
    28801 ttgagaaaat gtaatccatg atttttgaca atccaagaac acaaagaatc ccaccaattc
    28861 cgatcggcac aatcacttca agtcgtactt ctttaaatcc gtcagccatc gctttataca
    28921 atcccatata caccaaaaaa ttagaagggc tcaatcctgg gacgatcata cccaaaccaa
    28981 tcaatccgcc ggcaatcagc catgttccaa aattctgatc gacttgcccg tcaaataatt
    29041 gagcgccgta taataaaaac aaataagcca gaacaaaagt aacaaccatg atgatcacat
    29101 cagaagtatc tcttcctttt ttccctgctt ctttccataa cgcaggaaca gttccagcga
    29161 tacatccgac aaaaaaccat aatatcgttg tttcatacgc acctaataaa aaactgacta
    29221 aaaaagaaag tagaaagatg cctgtcactc cgcctagacc aacaggcaga aaaaacaaaa
    29281 cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga aatcagccgc tcatacatgc
    29341 caaagacagc tgctaatgca ccaccgctga ctcctggcaa gataaatcct gaaccgataa
    29401 acattccctt cacaaaccgt aacatccagt ctttggttgt gtaccccact tcttgatttt
    29461 ccattttttg ccttttttcc actatttgcg tactccttct ttcatttaat tttattcatg
    29521 attgaacatt tcctgctatt ttacttaaag actttttcat aggtctctat tattttttca
    29581 ttcattttga caatatcagg ataaaacatg ttacctccat tatgatagag atagccgcca
    29641 ttgtaaagca aggactggat ccgccaatat cggtattgtt tcccttcctc atttcctaaa
    29701 agtgggctca gcacatcttt agaatacgct tctgctaatt ctactgtgtt ttctccgcca
    29761 tgatttttta cataggcaat ataatctttc ccaaaattat atgcttggac agctgtccat
    29821 aaatcgcatc cttgtttctt tgcttctact atcatttccg ccaaatattt gacaccttga
    29881 tcaatactgt cagctggatt atcaatgatc tcagcttctc catgcagact ttccgagctc
    29941 tgcatgagat ctgtcccttt tccttttgat tcggtcaata ttatcgcctt gatcaaattc
    30001 tcgtagtttt ccatatgata attttttgca gctgttgtta tttctatttg atagctttcc
    30061 actcgtttta ccgaacgata agttcttcct cataaaaaca acaccgcacc aataaccgtc
    30121 agaatgatca tgtaaaaaag aatacggata atagataatc gttttcgctt catttcatca
    30181 cctagttatt ttgacatctt tttttatctt actatttctt tatcttcttg gctatttctt
    30241 ccttgatttc tgtgtacgcc ttaagaaaaa atgaattttc ctgtattttt tgacatctta
    30301 cgaaaatgat cgttgttttt cagtaaaaac aattgcccaa tgactgatat aatcgttata
    30361 ctttacacag taggtgatta tgtgaaaaaa gaaaagaaat atgcaaaaat gccagatggg
    30421 agcgaaattt actatgaaaa gagcggtcaa ggcttccctc tctttttact gcatggcaat
    30481 gacggcagcg gccgcttttt ttcagaacag gtccctgtac tcgaacgcta ctataccgtg
    30541 tatctagtag atagccgagg acatggtcgt tcgacaaacg aagcatctat gttgaatttt
    30601 cagttaatgg ccgaggatct gaatacgatc atgttgctgg aaaaaatcga tcaggcagat
    30661 tttcttggat ttagtgatgg agcaaatcta gcactggtat ttgccagcag cttccctaaa
    30721 aaagttcatc gattgatttt aaattctgga aacacgcttg ttaaaggcgt tcgtttttct
    30781 gccagagtca tcagtaatat tcattatgca tgggtatggt tactctcttt atttcgccca
    30841 agtcttcgca aaaatctttt agttatcaaa cttttgctgc atgacatcgg gcttacagaa
    30901 aatgatttaa aaaaaatcaa ctcccccact ttgatcatcg tgggaaaaaa agatgtaatc
    30961 aagctaaaac attctttgta tatagctaaa actataccaa aagcttcctt tgtgcttgtg
    31021 aaagaacaag gacatgaatt agctagaaaa gatcctgaac gcttcaatcg agaagttctt
    31081 caatttcttt ctgaaacata actattagga atagaaggtg atttatttgt taaaaaatac
    31141 catacaatgg ctgaaaaacc acctaggact gttcaaaaca atctttttga tttctgtcat
    31201 tgtcattatt gtcggtgaat tgatgtccat cggtaaaact ctatctatcc agcaattagg
    31261 agaaacgttt gccactatac ctgtatggaa aagcggacta atgctattga ttggtcttat
    31321 ctctgtgtta cctatggttg gttatgatat tattttgaat cggatgcttg aacaaaagca
    31381 gaatcctcgt tacctgtttg agacaagctg gctgatcaat acgatcaaca atattgccgg
    31441 tttcggcggg tttgttagtg tggggcttcg ctctgaacta tatggacaaa agaaagagag
    31501 cagaaacgtg atccaagcat tatcgaaagt tttcttgttt ttgatggctg gactttctat
    31561 ttacagtctg ctttcttttt tacttgtagt cttcacgcca gttgctcctt ttttgaagca
    31621 atattggata tggttgatcg gcggcggtct atattttcct gtcgtctttt tatttactac
    31681 tttcaaaaag aaaggattcc ttggtggtac ctcacttaaa actcaaggac agctattgct
    31741 agtttcattg ttggaatgga ccggtgtcct gtttagtttc ctttctgttg gttatttaat
    31801 ggaaatccgg attgatctgt ggcaaacaat tccattattc attgctgctt cagtgatagg
    31861 aatcgtgtct atgattccag gagaaattgg gagtttcgat gtaatgatga ttatcgggtt
    31921 gtccgctatt ggtgtgccaa gagaaatagt cgttgtgtgg atcttgctct atcgcctgtt
    31981 ctactacatc attccgtttt tgattggaat cgtctttttc ttcaaaaata tcggttccac
    32041 ttttgatcag cgttattctg gtatccctaa acaattagca acagaaattg cgcataaaat
    32101 cgtggttgtc ttattatact tttctggaat catgctagta ctttctgcca ctatcccgca
    32161 agcttttaca gagtttcgct ggcttcatag tttgaaccca ttgaaatttc attttattat
    32221 ccaatttcct agtatactat tgggatttct tttaatcatt atgggcagag ggatcgcagc
    32281 acgtgtgaaa cgagcatatt tgcctacaat cttcttgatt gttctagctt tactttatgt
    32341 tttattaagt gattttagtt tcacaccggt ggttttcttg tctattctgc ttctgatcat
    32401 tcttgcttct aaaaacgaat tatttcggga acaattgatc tattcatggg agtggcgtac
    32461 gatcgatgga atcattattg gtgcactgtc cttgctttac atcgttattg gggtatacaa
    32521 tctgccggat ttccctcatc ggcgtcacca ttttatttcc tttttcttat ttccttctga
    32581 aaagatttgg ttttcgggat tgttggccat catcgctgtt agtttcatga tcgttctatt
    32641 tgttcatttc cttcaaggag aaaagaaaca gattggcgaa gcctttaatg aagaaaaggc
    32701 cctgaagatc cttacaactt atggaggaaa ctccgatagt caattgatct tcttaaaaga
    32761 taagcggatg tttgcctatg aaaaagacgg tgaaccaact gttcttctcc aattcgcttg
    32821 cttcaataat aaatgtattg taatgggtga tccttctggt aagaaagaag attttccaga
    32881 agcaatcgaa gcttttatcg aagaaacaga tcgactatgc tatttaccag ttttctacga
    32941 aacaagcgaa gagattgtta tgattttgca tgaattcggc tacgatttca ttaagatggg
    33001 ggaagaagct tatgtagacc tgaatagttt tacaacttca gggaaaaaaa tgaagggcac
    33061 tcgtgctgtc ttgaatcgga tcgaacgaga aggatttaca tttgatgtct tacaaccacc
    33121 attttctact gagcaaatga gtactttcaa aaatatctct gataattggt tgggttcacg
    33181 aaaggaaaaa gggttttctt taggcttttt ctctgaagat tatctacaaa gagcgcctat
    33241 tgcagttgtc aaaaataccc aagaagaaat tgtcgctttt gcaacgatca tgcctactta
    33301 cacgaacaat aaagtaggaa cgattgatct gatgcgatat gattctaaaa aagcaccttc
    33361 tggcagtatg gatttcttgt tcttgcattt gtttgcctat atgaaagaag aacatatcca
    33421 atggttcaat ttagggatgg cccctctttc aaatgtcgga acttccagaa agagtttttt
    33481 acaggaaagg attgcatact tggtttacga gtttggttct catttttatt ctttccatgg
    33541 attaagagaa tataaaagca agtacgctac aaactgggtc tcacgttata ctctatattc
    33601 tagagatagt tggattgcat atgttatgat tgctttgctg attattgata atgcgccagt
    33661 tgagcagaca caaggtagaa taagtggttt gaaaaaatgg atacgaagaa agtattaaaa
    33721 actgcatacc gatagatgaa cactctcatg atgaataaaa gagactgaag taaaacgaaa
    33781 gttcatgctt ctacaatctc ttcttataca attgaagaac aaagagcggt ccaaacagaa
    33841 atcctgtttg gaccgctctt ttattttaaa catttttcat taccaaaata ttcgagtcgc
    33901 gtttcttatg cttcgatctc tgtaacgata cctgaaccta ctgtccgtcc gccttcacga
    33961 attgagaatg tcgttccgtt ttcaacagcg attggatgga tcaagtcaac atcaatcgtc
    34021 acgttgtcac caggcatgac catttctgta tcttctggca atgtgattgt tcctgtaaca
    34081 tcagttgtgc ggaagtagaa ctgtggacga tagttgttga agaatggagt atgacgtcca
    34141 ccttcttctt ttgtcaacac gtacacttct gctttgaatt ttgtatgtgg agtaatggaa
    34201 cctggtttag caagtacttg tccacgttcg atatcgtcac gttggatccc ccgtaacaac
    34261 acgcctacgt tatccccagc ttccccgtaa tctaacgttt tacggaacat ttctacacct
    34321 gttacaactg ctttttgtgt ttcaggtttg atccctacga tctctacctc atcaccgaca
    34381 cgaacagcac cacgatcaat acgacctgag gcaacagttc ctcgacctgt aattgagaag
    34441 acgtcttcca ctggcaatag taatggttta tctgtatcac gttctggtgt tgggatatat
    34501 tcatctactg tatccatcaa ttccataata gcagcttcag catctggatc gccttgcaat
    34561 gcttttaatg cggaaccttt gatcacagga gtgtcatcgc ctgggaaacc atattcgctc
    34621 aataactcgc gaacttccat ttctaccaaa tcgatcaatt cttcatcatc gactaaatca
    34681 actttgttca agaaaacaat caggtatttt acaccaactt ggcgtgacaa taaaatgtgt
    34741 tcacgtgttt gaggcatcgg accgtcagtt gcagaaacaa ctaagattgc accatccatt
    34801 tgtgctgccc ctgtaatcat gtttttcaca tagtccgcgt gtcctggtgc atcaatatgc
    34861 gcgtagtggc gtttttctgt ttcatattct acgtgtgcgg tattgatcgt aatcccacgt
    34921 tcacgttctt ctggagctgc atcaatacta gcatagtctt gaggatttgc taggcctttt
    34981 ttgcctaata ctgtagtgat cgcagcagtt aaagtagttt tcccatggtc aacgtgtcca
    35041 atcgtaccaa tgttaacgtg tggtttactt ctgtcataat gttcttttgc cataatttac
    35101 aaacctccta aataatatag atcactgaaa aatttcagct ctcacctttt tattatagtc
    35161 tttttttgac taaatcaaaa agaatacgtc cgacttactt tttgtaagat tcgaactaag
    35221 cttattatac gtcaatattg gtcaaagtca actaagagca ctcgtctgtc ttatacgcga
    35281 aaaaagtacc ttttataaat aaaaaaatgt gttttttgag aaaaacttca agatagtcct
    35341 taactttctt ttgaagcaat aagaacaaaa tgaggaaaag tttaaaattc agctgttgcc
    35401 aacaactttc aatagacgaa atcttctatt tcgttaataa taaggaaaga ggtgaagtac
    35461 atgaaaaaat taattggcgt acttgtcgtt ttaggcatat ttctcggcgg aagttttgca
    35521 gcttatcatt atttttacgg cggcgaagct tattatacaa aaatcacgac gaatggcgaa
    35581 atgtcagcta cccaagcaga taacggagag aaattcacga cttataccta caagcaagcg
    35641 gcttataata aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag agaacatcca
    35701 ttaaagatga atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt attaagctgg
    35761 gaagaaataa aggcttctga agtgcctaaa aatgcggcag aaaaaatcga taattagtaa
    35821 atggtcttaa aattttatta gataaaa
//
LOCUS       DAZUKT010000020        35443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000020 DAZUKT010000000
VERSION     DAZUKT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.020"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..688
                     /locus_tag="ACG816_000979"
     CDS             17..688
                     /locus_tag="ACG816_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919888.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            747..1187
                     /locus_tag="ACG816_000980"
     CDS             747..1187
                     /locus_tag="ACG816_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919889.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1199..1867
                     /locus_tag="ACG816_000981"
     CDS             1199..1867
                     /locus_tag="ACG816_000981"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4919890.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1931..2554
                     /locus_tag="ACG816_000982"
     CDS             1931..2554
                     /locus_tag="ACG816_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4919891.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2610..2996
                     /locus_tag="ACG816_000983"
     CDS             2610..2996
                     /locus_tag="ACG816_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4919892.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3209..4600
                     /locus_tag="ACG816_000984"
     CDS             3209..4600
                     /locus_tag="ACG816_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4919893.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5211..5579
                     /locus_tag="ACG816_000985"
     CDS             5211..5579
                     /locus_tag="ACG816_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4919894.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5759..6700
                     /locus_tag="ACG816_000986"
     CDS             5759..6700
                     /locus_tag="ACG816_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4919895.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6769..7500
                     /locus_tag="ACG816_000987"
     CDS             6769..7500
                     /locus_tag="ACG816_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4919896.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7644..9824
                     /locus_tag="ACG816_000988"
     CDS             7644..9824
                     /locus_tag="ACG816_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4919897.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9844..10326
                     /locus_tag="ACG816_000989"
     CDS             9844..10326
                     /locus_tag="ACG816_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4919898.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10342..11103
                     /locus_tag="ACG816_000990"
     CDS             10342..11103
                     /locus_tag="ACG816_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4919899.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11234..12568
                     /gene="murC"
                     /locus_tag="ACG816_000991"
     CDS             11234..12568
                     /gene="murC"
                     /locus_tag="ACG816_000991"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4919900.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12875..13360
                     /locus_tag="ACG816_000992"
     CDS             12875..13360
                     /locus_tag="ACG816_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4919901.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13353..14036
                     /locus_tag="ACG816_000993"
     CDS             13353..14036
                     /locus_tag="ACG816_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4919902.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14162..16807
                     /gene="polA"
                     /locus_tag="ACG816_000994"
     CDS             14162..16807
                     /gene="polA"
                     /locus_tag="ACG816_000994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4919903.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16852..17688
                     /gene="mutM"
                     /locus_tag="ACG816_000995"
     CDS             16852..17688
                     /gene="mutM"
                     /locus_tag="ACG816_000995"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4919904.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17652..18281
                     /gene="coaE"
                     /locus_tag="ACG816_000996"
     CDS             17652..18281
                     /gene="coaE"
                     /locus_tag="ACG816_000996"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4919905.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18333..18665
                     /locus_tag="ACG816_000997"
     CDS             18333..18665
                     /locus_tag="ACG816_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4919906.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18977..19468
                     /gene="nrdR"
                     /locus_tag="ACG816_000998"
     CDS             18977..19468
                     /gene="nrdR"
                     /locus_tag="ACG816_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4919907.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19491..20864
                     /locus_tag="ACG816_000999"
     CDS             19491..20864
                     /locus_tag="ACG816_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4919908.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20864..21790
                     /gene="dnaI"
                     /locus_tag="ACG816_001000"
     CDS             20864..21790
                     /gene="dnaI"
                     /locus_tag="ACG816_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4919909.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21919..22359
                     /locus_tag="ACG816_001001"
     CDS             21919..22359
                     /locus_tag="ACG816_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4919910.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22503..23204
                     /locus_tag="ACG816_001002"
     CDS             22503..23204
                     /locus_tag="ACG816_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4919911.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23201..24322
                     /locus_tag="ACG816_001003"
     CDS             23201..24322
                     /locus_tag="ACG816_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4919912.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24337..25569
                     /gene="pepT"
                     /locus_tag="ACG816_001004"
     CDS             24337..25569
                     /gene="pepT"
                     /locus_tag="ACG816_001004"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4919913.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25701..26609
                     /locus_tag="ACG816_001005"
     CDS             25701..26609
                     /locus_tag="ACG816_001005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4919914.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26644..26916
                     /locus_tag="ACG816_001006"
     CDS             26644..26916
                     /locus_tag="ACG816_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4919915.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26927..27973
                     /locus_tag="ACG816_001007"
     CDS             26927..27973
                     /locus_tag="ACG816_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4919916.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28224..30833
                     /gene="clpB"
                     /locus_tag="ACG816_001008"
     CDS             28224..30833
                     /gene="clpB"
                     /locus_tag="ACG816_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4919917.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30984..31670)
                     /locus_tag="ACG816_001009"
     CDS             complement(30984..31670)
                     /locus_tag="ACG816_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919918.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31648..32142)
                     /locus_tag="ACG816_001010"
     CDS             complement(31648..32142)
                     /locus_tag="ACG816_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4919919.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32162..33382)
                     /locus_tag="ACG816_001011"
     CDS             complement(32162..33382)
                     /locus_tag="ACG816_001011"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4919920.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33529..35364
                     /gene="lepA"
                     /locus_tag="ACG816_001012"
     CDS             33529..35364
                     /gene="lepA"
                     /locus_tag="ACG816_001012"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4919921.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11865 a         6250 c         7622 g         9706 t
ORIGIN      
        1 aaaaaggggg agaaaaatga attttttagt tgttttaaaa gatactctga ttgagcgatt
       61 tacatttcta tatgttcaat tgcaggaaaa taaaacattg ctgcattttt caccagagtt
      121 ccatctagag gggctaacat taggagaaat ctatcaagca gaagggctgt cggctgtttt
      181 gcactttttt aaggtagaat tggaattacc agtaaaagat tatatcgagc tttctcttga
      241 atcttgggat cgagcagtgg ttgaactgtt tccagaaggt ttaatgatcg atacaaacga
      301 tgggaagatc catctgactg cagcagaact gcaaaaacaa atgcgttatc aaatagatga
      361 cgaaaattct ttttctattt ttagacgtca gcaaaagatt ctaaaaagct ttttaaaagt
      421 gatcagtaaa aaacggaatc tgatcaaaac gacacagctg ttgaaaaaat atccaggtct
      481 gcttcacacg tctattcaat ttcctcaatt gattacgatg atccatcgtt ttatacgtat
      541 gcaacagcta ccatctaaga agctgtttct tccattgacg gacacatttc gagtagtcaa
      601 cagagaagat aaaaagaaag tgattgtgat tgactttacc agaaatcgga atgtccttca
      661 ccaagttgca atggaaaagg ccaattaagg ctttttcttt ttggattttt ttgtcataat
      721 aaatattaga tgatggagga gataatatga tgcaaacatg gttagaaagg ctaatacgat
      781 ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc gaaatttgat cagttgaatc
      841 gcggtctatt gatcgctgcg cttgtcttta gtttgctaag tcgttggttg ccctatagaa
      901 tcggacaatt gttgttcatc gtctttttcg gatggatgat atttcgtttc ctatcaaaaa
      961 aaatctaccc cagactaaat gagaatcaac gttatttgaa atatcttgat agagtcaaag
     1021 aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat ggatcgaaaa agttatactt
     1081 tttttgagtg cccgaattgt catcaaaagc agcgtgcacc taaaggaaaa ggacgaatac
     1141 gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa tgtttaaggg agaaaagaat
     1201 gaaattagta ttattacgtc atggcgaaag cgaagcaaat tttgaaaatt actggacagg
     1261 atggctagat gtcgccttaa cagaaaaagg aaaagaacaa gcagcagttg ccggtcaaaa
     1321 aatgcttgaa gccggacttc aattcgatgc cgtttatacc tctgttttga aacgtgcgat
     1381 tcttaccgga caagtcgcac tagaagaaat gggtcagtta tggcttccta taataaaaac
     1441 atggcgtcta aatgaacggc attatgggga tcttgtgggt aaaaacaaag ataagatgaa
     1501 agaaatatac ggaaaagatc aagtaaaaac atggcgcaga ggatatacgg aagttccgcc
     1561 gttaaccgca gaaaatagat ttgaccgcag atatgatcac ttggatccgc atttgatccc
     1621 atacggagaa agtctggaaa tgacagtgaa tcggatcatt cccttatggc aagatcatct
     1681 agcaccaaaa ctaaaagatc ggcaagattt actagttgtg ggtcatggta atagcatccg
     1741 agcactgaca aaatatttag aagatgttcc tgaagatcaa atggatacga tcgatatccc
     1801 aaatgcacaa ccaatccagt atacattagc agatgatttg acaattctgg acaaaaaaat
     1861 actttaaaga acacctttat ctaaaagtga gagatagaaa aaagaagcaa aggaatggaa
     1921 caaaaggaac atgaaaaatt atatatggga ctttgatggt acattgtttg atacgtatcc
     1981 cgcaatggta gatggtgcat ggcaagcact aaaagatttt ggtatcagta tggacaaaaa
     2041 agaaatctac ttcaaaatga aaaagtattc tactagttat ttgatcaatg aatcaaactt
     2101 gaatgccaga gagtttaatg aattatttca ccgatatgaa aaagaaagta cggaagtttc
     2161 ccgtccattt ccagaaacga aacaagtact ggaaatgcta aaagacaacg gcggtcgtca
     2221 tttcatcctg acccaccgtt tgacagaatc aacatggggc ctgttaaaag aacatcgatt
     2281 ggcacatctg attgaggaag tagtagggat cgatcaggac tttcctcgta aacctgatcc
     2341 agcatcttta aattatttga tcgatacttt tcatttagaa agaacagaca cgatgatgat
     2401 cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca ggagtcgcta cttgtcttta
     2461 cgacatcgat cattttctgg gtgagatccc ggcagattat gtggtaggaa atctgaatga
     2521 aatccttacc ttgcagagca tcattaaaaa gtagtatccc ctttcaaagg agatatgtta
     2581 taattttcat aaaggagtga taaaattata tggaaatcag aagacaaaaa gaagttgtag
     2641 aagcatttca ttatgatatg agattgccga accaagaagt agaaacagat ttgaaagtag
     2701 gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa agaacatagc atcgtaggag
     2761 cacgcttaga gttccgttta gtgtttgata catatgtttt atctggaagt gtgagtcaga
     2821 tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga tgtcacacaa gaagaagtgg
     2881 acgagctagt agcaccgtta ttcgatatcg tacgccgaat gtcttatgag gtgacagaga
     2941 tcgccttaga tcagcctggg gtaaaattga attttcaatc agaaaaacaa gaatagacag
     3001 taaaaacata cctgcgatag attaaagcta tcacaggtat gtttttttat tactaattgt
     3061 aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat ataaaagtca ctgaaaaaaa
     3121 ttttttcagg aggatggttg aagaatcatt ctcgggaacc tttacttcct tcgatggact
     3181 tgtcattaat aatatgagga ggagaaaaat gacaacactc atcacagttt tagtggttct
     3241 agtttttgcg gcattattat atgtgttcta tcgtatgcaa aagcgccata caaaattttc
     3301 aacacgcgtc tttacagcat taggagttgg tatcgtcttt ggcggtctgc ttcaattgat
     3361 ctttggtgta gacgacaaag tcgttactca atcaatggaa tggatcggga ttgtaggtca
     3421 aggatatgta tcattcttgc aaatgctagt tatgccttta gtgtttattt cgatcattgg
     3481 tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa attagtgcga ctgtattgac
     3541 ttctttactt ggaacaacag ctattgcagc attgatcgga attggttcaa caatgctgtt
     3601 cggactccaa ggagctaaat tcacacaagg agctgcagaa acagcacgaa tctctgaact
     3661 ggcaacacgt caggaaacag ttgaaaactt atcgattcca cagcagattt tatcctttat
     3721 ccctaaaaac gtttttgctg atttagcagg tagtcgtgca acaagtacga ttggagtcgt
     3781 tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg aaaagaaaag cgccaaaaga
     3841 agcagcattc ttttcagatt tagtcgacag tctgtataaa atcattatgc ggatcgtgac
     3901 gcttgttttg cgtttgacac cttatggagt atttgctttg atgacaaacg ttttggcaac
     3961 aagtgatttt gcagctatcg tcaatttagg taaatttgtc gttgcttctt acgtagcatt
     4021 gcttgctgtt ttgattgtcc actcactgat tttgattggt gcaaaagtca atccaatcaa
     4081 ctatttcaaa aaagcctttc cggtattgag ctttgcgttt acttcacgtt caagtgctgg
     4141 agctttgcca ttgaatatcg agactcagac aaaagcttta ggagtagatg atgcaacagc
     4201 taatttctca gcaagttttg gtttgtctat tggacaaaac ggctgtgctg gcgtttatcc
     4261 agctatgttg gcaacgatcg tcgcaccagc agctggagtg aatgtcttta gtttggaatt
     4321 tattttgatg cttgtggcaa ttgtaacaat cagttctttt ggtgtagctg gagtcggtgg
     4381 gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg aacttgccag tagcaatcgt
     4441 tgggttagtt atctctgttg aaccattaat cgatatggca cgtacagcag taaacgtcag
     4501 cggtagcatg gtagctggta tcgtgacaag tgcacgcatc aaagatttag atcgcgatgt
     4561 catcaatgat gattctaaag tgatcgatgt gaatgcatag tcaaaaagag gttgtgatag
     4621 aagtggtcag ctttaagaaa taagaagaag tttcctaaaa atagtttctc aaatttttgt
     4681 gaaatttcgg cttatttccg agaagctact tctgaaacac cgtgaataag aggttgtgaa
     4741 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
     4801 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgtttaag aaagaggtcg
     4861 tgagcctgcc gtttcactct gagtcacaaa gagcaatttc aaaaaatagc ttctcatgtt
     4921 tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag aacaccgttt accagaggtt
     4981 gtgacttgtt tttagtcacg acctcttttt ttatttattc catataaata cacattattt
     5041 attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac ttatttatta ttaaagaaaa
     5101 gcgtatttaa gtaggacgat tctgttgaga gctttcgtag attttcccat tttcctatgg
     5161 taaactgaca aagttgagaa tgttagaaca gatcgtttag gaggaaacag atgtttacga
     5221 atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg ttttggagag
     5281 cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata attgaagtag
     5341 gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt gagtcccatt
     5401 cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat atctttggac
     5461 tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag cttggagaag
     5521 aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca ggtaaataat
     5581 agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt taattcaaat
     5641 aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca aataaatcgt
     5701 caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga agaaatacat
     5761 ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag aggaatttgc
     5821 caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc tagtagctga
     5881 agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct atatgaaagc
     5941 aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa gcgaaggaca
     6001 tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa agctagaaga
     6061 ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat ttaaagaagc
     6121 tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat atgacttggg
     6181 acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc cagactggat
     6241 ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta ctggtggcat
     6301 tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg tcggacagct
     6361 gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat tatgggacgg
     6421 caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag ataaacaaat
     6481 cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt gtagcaatcc
     6541 tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt taggagcatg
     6601 tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg gaatctctcc
     6661 agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag ttttgattgt
     6721 cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat gaaatcattg
     6781 atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact tattcactat
     6841 tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg aggtcctcgt
     6901 acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc catttacctc
     6961 cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga ctggtatgcg
     7021 ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc aggggcatcg
     7081 gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc agaactgatt
     7141 aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt tgtccatgcc
     7201 ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt tatttggata
     7261 agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt tggacatacg
     7321 attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca ggatcataaa
     7381 atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt ttttgacaag
     7441 gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca tggaatctga
     7501 ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt ttatagagaa
     7561 tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa agaagcaatt
     7621 tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc attcagcttg
     7681 tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt aatttgttaa
     7741 atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact ggaagtcttg
     7801 atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac ttagaaaaac
     7861 gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc gagttagaag
     7921 cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt ccatacaaac
     7981 aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca ctcgcgaagt
     8041 ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat attgacaaag
     8101 aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt attgctgaac
     8161 aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat aacaaaggta
     8221 tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat gaaatgtatt
     8281 atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct actaatcgtg
     8341 gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca attttagctt
     8401 acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc gtcagagaag
     8461 cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa ctgcgcaatg
     8521 aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac ctccgtaatc
     8581 ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg gcttatcgta
     8641 ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc gaagttattt
     8701 atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct tttatccaat
     8761 taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc agtcgggaat
     8821 ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat tacgctatcg
     8881 tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa gaatttccgc
     8941 atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa gatcctttag
     9001 ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa catgacgttt
     9061 ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt aaccaagtag
     9121 gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga ttgaataaaa
     9181 caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc gcacgaactc
     9241 aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc ggattcttgc
     9301 gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa agttattcta
     9361 ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt acagaagaag
     9421 cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta tcagtcggag
     9481 aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat atgcgtgatg
     9541 agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg aaaccaggga
     9601 tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt gatatcggtg
     9661 tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc aagcatccta
     9721 cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc gatacgaaaa
     9781 aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga ggagtaacaa
     9841 aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa aagcttccag
     9901 cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg cgggcgctat
     9961 catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc agacgaagag
    10021 acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct agctggaaga
    10081 gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg agggttgcgc
    10141 tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg ccaagattgt
    10201 tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg tggaagatgt
    10261 catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc acaaaaaatc
    10321 agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa agactggaat
    10381 cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact gacggcacct
    10441 acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc acaaatgtat
    10501 ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc gactagaatc
    10561 ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag tgaaagtgaa
    10621 atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt tctgatagaa
    10681 tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct tcattcctta
    10741 ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg caggactcga
    10801 gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc tgtgccattt
    10861 gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga actgaatcat
    10921 caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg tctccatgac
    10981 gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga tatatcagag
    11041 gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt ttctcgtact
    11101 tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat tttcaatcgt
    11161 ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg ctagataaga
    11221 gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta tcaaaggttc
    11281 aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc aaggatcaga
    11341 cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca cgattctgcc
    11401 atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg catttcctga
    11461 ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc gctatcacga
    11521 ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct ctcacggaaa
    11581 aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa caagttatct
    11641 aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat ttgaggcctg
    11701 tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga ccaatatcga
    11761 ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt tccaaacaat
    11821 ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt tacgcaaatt
    11881 aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata tccaagcacg
    11941 aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg atgaatttgt
    12001 tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt taggtgttat
    12061 tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa tgctgacttt
    12121 cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg tagtcgatga
    12181 ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc aaaaatatcc
    12241 agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga ttgcattgat
    12301 ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg atatttttgg
    12361 ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga aaatcaaaaa
    12421 aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc atgatgcagt
    12481 agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg aaaaactttt
    12541 aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta taaaaataga
    12601 aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat gataaacggt
    12661 gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg agaagctgtt
    12721 ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct ctgtaaacta
    12781 taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt ggtacaataa
    12841 tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc cgagaaaact
    12901 aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag aatctattga
    12961 agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt tgtatatcca
    13021 acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag tgatgctgat
    13081 ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac atgttgtcaa
    13141 tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt ttcagtttga
    13201 ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag cggagaatac
    13261 gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag ttcctgaatt
    13321 ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca tcaatgtcag
    13381 gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt gcccttagtg
    13441 cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact tttatcaata
    13501 attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta atggttctag
    13561 gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc tactctcttt
    13621 tgaatggcat aacattagcg gttactcttg catactacga tattggaaca gtaacgcaag
    13681 ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta acaacaaaaa
    13741 gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg atcattgcac
    13801 tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc tccctactgt
    13861 tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt aatatctatg
    13921 tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg aagctgtatt
    13981 tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac aactaaaaaa
    14041 gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg ctttttcttt
    14101 tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag gagttaagaa
    14161 gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc gcgcattctt
    14221 tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata caaatgcaat
    14281 ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga ctcatgttct
    14341 ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg agtacaaagg
    14401 tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac gtgatttgtt
    14461 gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg atattatcgg
    14521 tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt caggtgatcg
    14581 ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa aaggtgtcag
    14641 cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt tgacgccaaa
    14701 acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc ccggtgtgac
    14761 taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag tcgaaggaat
    14821 ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga taaatgacaa
    14881 agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc ctgtggatgt
    14941 ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc cgttttataa
    15001 agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag aagtaaaaat
    15061 ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta ctacggatat
    15121 ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg tcggagtagc
    15181 gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg aaaaccgagc
    15241 gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg cgaaacgaac
    15301 ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg atgtcttgct
    15361 aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa tagctgctca
    15421 ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg cgaaaaaagg
    15481 tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag ctgcaatcaa
    15541 tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag atcttttccg
    15601 gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg ggatcaaagt
    15661 agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa gagagatcga
    15721 acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa agcaattagg
    15781 agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa ctggatattc
    15841 cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag aggatatcct
    15901 cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc tgaaagtgat
    15961 ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa caggacgatt
    16021 aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag ggagaaagat
    16081 acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg actattcaca
    16141 aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag ctgccttcct
    16201 agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg aaaaggcaga
    16261 ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta tcgtgtatgg
    16321 catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag cacagcaata
    16381 catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa gaatcgtacg
    16441 cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat atctgccaga
    16501 catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta tcaatacacc
    16561 aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg ataaacgatt
    16621 aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac ttgtcttcga
    16681 ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta tggaacaagc
    16741 tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt ggtacgaagc
    16801 aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa aatgccagaa
    16861 ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg aaaaacgatt
    16921 caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc catctttgag
    16981 gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt tttaattttt
    17041 catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata tcaatttacg
    17101 aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga ggatggaagc
    17161 caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga aaaaggagca
    17221 agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga tagcttctta
    17281 ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc attgctgcta
    17341 gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt atgggaagcg
    17401 aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga acaattacgt
    17461 ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac gattcgcagt
    17521 tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt ctatcagcaa
    17581 actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt ggcacaaagg
    17641 gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga tttgttttag
    17701 gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt cagtctcatg
    17761 gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct ggaacgcttg
    17821 ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat gggcaactag
    17881 atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa aaacttgatc
    17941 agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct ctaagttcca
    18001 atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat gaccactata
    18061 tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt ttgatgaaac
    18121 gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt ccaatcgaag
    18181 aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa aaacttgtgg
    18241 gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg tcaattttgc
    18301 tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat caaaaggata
    18361 aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa aatctaatcg
    18421 atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac caagcaagaa
    18481 tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat ttgactcaag
    18541 aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt gaccacattc
    18601 ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc gaagagaagt
    18661 tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt tcatctgctt
    18721 caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct cagtaatcaa
    18781 ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt acgcatgaaa
    18841 gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt ttgtgatttg
    18901 tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata aaatgcgatg
    18961 caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac gagtaatcga
    19021 cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg agaattgttc
    19081 gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa tcaagaaaaa
    19141 cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc gttcagcaga
    19201 aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg aaaaccgcgt
    19261 acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg tgatggaaga
    19321 tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc gccagttcaa
    19381 agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa aagaaaataa
    19441 taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc ttggaaaatg
    19501 cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct ccgctgagag
    19561 aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga gaagccttga
    19621 gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt cctgaaggga
    19681 tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat gatgcaagaa
    19741 aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt gatctgggac
    19801 ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc aaagactctg
    19861 ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag cttacccaat
    19921 tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa aaatttttag
    19981 atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc gaatcgctga
    20041 ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg acattcgatt
    20101 ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat gatccaatca
    20161 tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca cttggcgaac
    20221 tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa ctgcaaaaag
    20281 ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt gcggtttctg
    20341 aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt cagctgataa
    20401 aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca gaaaaaggcg
    20461 gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa tcaggtcttt
    20521 ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat aatgctagct
    20581 taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa atcgccacag
    20641 cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa tcaaaacaaa
    20701 agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag cttcctgatt
    20761 gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca gagattgacc
    20821 gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag acgtaggcaa
    20881 agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg aaatgatcgc
    20941 tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag aagaattgac
    21001 acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag aaaagcgtaa
    21061 atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc tgacgttgaa
    21121 ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac ggcaaagaca
    21181 agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc aatcggcttc
    21241 ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag ccctagattt
    21301 tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc tagtaggaag
    21361 ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag cgattgctgg
    21421 attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac aggcaatcgg
    21481 aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt tgatgatcga
    21541 tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg gtgttatcct
    21601 tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta caatggatga
    21661 attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa aagcgaaacg
    21721 gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc gcaaccgacg
    21781 caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa agcaggtcag
    21841 cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta atgcagaaac
    21901 ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt tggtctattt
    21961 tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga ttcgattgat
    22021 gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat atttgtcgga
    22081 acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt gaaactcgtc
    22141 gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa tgacggagtg
    22201 atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa aatcggtgac
    22261 aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc accgcaagtt
    22321 cctggagatt cgattattga tgtttcattg aacaaataac caataacatt ttttcaaaaa
    22381 taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata tgggaatttt
    22441 tttattccta gtctccttat tttatttgga aaatgctata attatctaga aggagtgaag
    22501 ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt gtccctgaat
    22561 ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca ttgatattaa
    22621 gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat gaatcagcac
    22681 gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt gcaaatggtc
    22741 tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt atgggaggtt
    22801 cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc ggacaagaac
    22861 gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg caagaaaatc
    22921 agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat gaaatcattg
    22981 tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg ttcgggccat
    23041 ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag cttcaacaaa
    23101 gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt caaaaacaaa
    23161 tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca gcggacgcac
    23221 tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag gagacccagt
    23281 cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga cattagacgt
    23341 tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga ttgctaaaca
    23401 tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag aaaaaatgta
    23461 tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca tggatattat
    23521 ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg agagttattt
    23581 agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg tggatcatgc
    23641 acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg attataccgg
    23701 tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg cacaaccagc
    23761 gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg ttattttgcc
    23821 ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt atgaggaacc
    23881 tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag gacgagtagg
    23941 tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag aagcgttcca
    24001 actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga agcgcattgc
    24061 gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa gagcagatgt
    24121 ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg ctgggttgat
    24181 tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta tcgagaaatt
    24241 tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg aaacaaatac
    24301 aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct tttgccacgt
    24361 tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac gactccatcg
    24421 actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga gttaggctta
    24481 gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc aagcaatgtt
    24541 gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga ttttaatgca
    24601 gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat cgtactagat
    24661 gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa ttatgaaggc
    24721 cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa atcagggatt
    24781 gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc tcacggtacc
    24841 atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa attcgatgtt
    24901 gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg tgagttgcag
    24961 tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa tgtacatcca
    25021 gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt tcataatcag
    25081 cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt ccatttgatg
    25141 gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga tcatgatcgt
    25201 gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt gaatcagcga
    25261 tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat gaaagaaatc
    25321 attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga gcttggtatc
    25381 tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc ttacatgggt
    25441 attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt cgaatttgtt
    25501 tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga attgaacaca
    25561 aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat gatggaatgt
    25621 catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct cttttttgaa
    25681 gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa agttcaggga
    25741 aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa aagcagccag
    25801 aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa ttagtcaaga
    25861 aagcagaagc catagaagct gatactttag taattatagg tggagacggc acgattcatc
    25921 atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta cttccaggtg
    25981 gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa gaagctgctg
    26041 acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat gatgaagtca
    26101 ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc agtcaagaag
    26161 aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta ttgctgaaga
    26221 agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga aaatcccaac
    26281 tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac gactcagcga
    26341 ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt ttccgattta
    26401 ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc attacttatt
    26461 tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact cgaacggatg
    26521 gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga ttaacagttt
    26581 attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt tggaagaaga
    26641 gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt gaatttttac
    26701 ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg gcgtgttcct
    26761 gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt gatctctcga
    26821 aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc tgcaggtgta
    26881 cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt cgtctaaagg
    26941 aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg ccattattta
    27001 ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta ccagtgttgt
    27061 tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag ggcgctcaat
    27121 caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt tcttgacctc
    27181 tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga tctcggagat
    27241 ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga cagcactcca
    27301 tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg tcatccaatt
    27361 ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg gtatcctttt
    27421 gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg ttcaaatatt
    27481 ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat gggtggatac
    27541 atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc aagacaggac
    27601 tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt atgtacttcc
    27661 ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca tttcgtttat
    27721 cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg gttcctttga
    27781 atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag tttcttcgct
    27841 tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg tgtatgtgat
    27901 tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag agaataaaga
    27961 aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag aaaaacgtta
    28021 tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa tcaattttgt
    28081 ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta tattttgacc
    28141 aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag tgatcttacc
    28201 tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg cttcaagaag
    28261 cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc gacattgccc
    28321 atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat acggatgccg
    28381 gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag tatccagttg
    28441 tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt cgattattga
    28501 atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta
    28561 ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat aaaaacggat
    28621 tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa agagtgacct
    28681 cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat ttagtacagc
    28741 aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca
    28801 tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa cccggtgtag
    28861 ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa
    28921 atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct ggagcaaaat
    28981 tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg
    29041 gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga aaaacagaag
    29101 gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga
    29161 tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa gcgttggaac
    29221 gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc agtattctgc
    29281 gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac aacgcattag
    29341 tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg gacaaagcaa
    29401 tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct atgccaacgg
    29461 aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct gcactgaaaa
    29521 aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac
    29581 ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt
    29641 ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa
    29701 ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct caattagaaa
    29761 aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg gttcaagagt
    29821 ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc cccgtaacga
    29881 aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt cataaacgag
    29941 tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg
    30001 gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca actggtgtcg
    30061 gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa gatcatatgg
    30121 tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta gtcggagctc
    30181 ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc
    30241 cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct
    30301 tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta gtagatttca
    30361 aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg gaaggcgtga
    30421 ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa
    30481 acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca ccattgagcc
    30541 tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa cgtctggaac
    30601 atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct gaaaatgctt
    30661 atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa gtagaaacac
    30721 cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta acgattactt
    30781 tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc taatcttcca
    30841 ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc cttttttcaa
    30901 ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct tgaagctgcc
    30961 cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt ataactttca
    31021 aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa ggcagcacct
    31081 atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt
    31141 gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc gcataagaat
    31201 tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa taagaggaac
    31261 aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat caatccatag
    31321 ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca aaaagagatg
    31381 atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa
    31441 aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg
    31501 aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg agaagtcgaa
    31561 tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc aaaagcaagt
    31621 aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat ctaaaaatct
    31681 ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca atttgatcaa
    31741 acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc
    31801 gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc cgatcactgg
    31861 gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga tcgtcagccc
    31921 atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag caagagcaat
    31981 cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt gaggaagata
    32041 gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt tatataacaa
    32101 atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat catccttttt
    32161 atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct agtgacttca
    32221 gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca cggcgataat
    32281 tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct ataggttcat
    32341 ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca ttattccaag
    32401 gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca aacggaaacc
    32461 gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg atctgctctt
    32521 tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata tctccatgta
    32581 cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt cgagcttctt
    32641 gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag tagaacagaa
    32701 cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca tcttcatttt
    32761 cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca tggttttgtt
    32821 gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat cgctcaacca
    32881 taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag gaaagtttat
    32941 cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga tacaaataag
    33001 agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga tcttgcatca
    33061 atccatggtt agcatattct ggataagttc caactccagt cacaccaccc attttataac
    33121 gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc agtttatttt
    33181 cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa cggataaaag
    33241 ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg aacacatagg
    33301 aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat tcaagatttt
    33361 ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata acagtatagc
    33421 aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa attactgctt
    33481 ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat ggatataaag
    33541 aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca tatcgaccat
    33601 gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa
    33661 atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat cacgattaaa
    33721 ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat ctttcacttg
    33781 attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt
    33841 gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac attggccaat
    33901 gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa aatcgaccta
    33961 cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct
    34021 agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat tctcgagcag
    34081 atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa agctttgatc
    34141 tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat cacggacggt
    34201 atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt tgatgttaca
    34261 gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt cggtgatgtc
    34321 ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga tacagtaacg
    34381 ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt
    34441 tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga agctttagaa
    34501 aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca agcattaggt
    34561 tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca agaacgcttg
    34621 gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta ccatgttaat
    34681 aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga acctgtaacg
    34741 attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa tgattttgtc
    34801 ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat ggattattta
    34861 gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt ctttgacttt
    34921 ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga aatgtctggt
    34981 tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa agtcgatgca
    35041 ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa
    35101 ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc tattggacaa
    35161 aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat
    35221 ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg
    35281 atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt tctgaaaatg
    35341 gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa aaaataaacc
    35401 atgtttacat ggatttgcca ccggtttgcc accggtggtt ttt
//
LOCUS       DAZUKT010000021        34961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000021 DAZUKT010000000
VERSION     DAZUKT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.021"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="ACG816_001013"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="ACG816_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4919922.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="ACG816_001014"
     CDS             complement(2019..2921)
                     /locus_tag="ACG816_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4919923.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="ACG816_001015"
     CDS             complement(2918..3610)
                     /locus_tag="ACG816_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4919924.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="ACG816_001016"
     CDS             complement(3653..4294)
                     /locus_tag="ACG816_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4919925.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="ACG816_001017"
     CDS             complement(4332..4646)
                     /locus_tag="ACG816_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919926.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5263)
                     /gene="pyrE"
                     /locus_tag="ACG816_001018"
     CDS             complement(4634..5263)
                     /gene="pyrE"
                     /locus_tag="ACG816_001018"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS4919927.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACG816_001019"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACG816_001019"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4919928.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="ACG816_001020"
     CDS             complement(5983..6915)
                     /locus_tag="ACG816_001020"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4919929.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="ACG816_001021"
     CDS             complement(6912..7688)
                     /locus_tag="ACG816_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4919930.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACG816_001022"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACG816_001022"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4919931.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /locus_tag="ACG816_001023"
     CDS             complement(10932..12008)
                     /locus_tag="ACG816_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4919932.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="ACG816_001024"
     CDS             complement(12013..13293)
                     /locus_tag="ACG816_001024"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4919933.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="ACG816_001025"
     CDS             complement(13312..14238)
                     /locus_tag="ACG816_001025"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4919934.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="ACG816_001026"
     CDS             complement(14251..15534)
                     /locus_tag="ACG816_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4919935.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACG816_001027"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACG816_001027"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4919936.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="ACG816_001028"
     CDS             complement(16322..17233)
                     /locus_tag="ACG816_001028"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4919937.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACG816_001029"
     CDS             complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACG816_001029"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4919938.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="ACG816_001030"
     CDS             complement(17699..18397)
                     /locus_tag="ACG816_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4919939.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="ACG816_001031"
     CDS             complement(18384..18770)
                     /locus_tag="ACG816_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919940.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="ACG816_001032"
     CDS             complement(18917..20587)
                     /locus_tag="ACG816_001032"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4919941.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="ACG816_001033"
     CDS             20770..21003
                     /locus_tag="ACG816_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4919942.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="ACG816_001034"
     CDS             complement(21102..21533)
                     /locus_tag="ACG816_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4919943.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..22025
                     /locus_tag="ACG816_001035"
     CDS             21612..22025
                     /locus_tag="ACG816_001035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4919944.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22063..23211)
                     /locus_tag="ACG816_001036"
     CDS             complement(22063..23211)
                     /locus_tag="ACG816_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4919945.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23333..23929)
                     /locus_tag="ACG816_001037"
     CDS             complement(23333..23929)
                     /locus_tag="ACG816_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4919946.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="ACG816_001038"
     CDS             complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="ACG816_001038"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4919947.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27159..27590
                     /locus_tag="ACG816_001039"
     CDS             27159..27590
                     /locus_tag="ACG816_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4919948.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27635..28984)
                     /locus_tag="ACG816_001040"
     CDS             complement(27635..28984)
                     /locus_tag="ACG816_001040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4919949.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29054..30217)
                     /locus_tag="ACG816_001041"
     CDS             complement(29054..30217)
                     /locus_tag="ACG816_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4919950.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30346..30669
                     /locus_tag="ACG816_001042"
     CDS             30346..30669
                     /locus_tag="ACG816_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919951.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30852..32201)
                     /locus_tag="ACG816_001043"
     CDS             complement(30852..32201)
                     /locus_tag="ACG816_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4919952.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32270..33223)
                     /locus_tag="ACG816_001044"
     CDS             complement(32270..33223)
                     /locus_tag="ACG816_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4919953.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33325..34515)
                     /locus_tag="ACG816_001045"
     CDS             complement(33325..34515)
                     /locus_tag="ACG816_001045"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4919954.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34715..>34961)
                     /locus_tag="ACG816_001046"
     CDS             complement(34715..>34961)
                     /locus_tag="ACG816_001046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919955.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9951 a         7642 c         6120 g        11248 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa aaaaagactc
    22021 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
    22081 gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt gactacgtaa
    22141 gattctttct agttctgtat caacaaactg gaatttctca gggaccacta catcaaattg
    22201 cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg tacctgtttc
    22261 aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat caacaggcat
    22321 tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt tttctatcag
    22381 atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga tgtggactga
    22441 tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc caagtatatc
    22501 atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg ggcgacttcc
    22561 taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga tatataatgc
    22621 taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga ttgctgctga
    22681 aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg tagctctcaa
    22741 tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg caaatttcaa
    22801 aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat tttctggatg
    22861 gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa tgatgatcga
    22921 aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt cctgatctgc
    22981 tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag tatctgatag
    23041 actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga tcattagctt
    23101 agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta ctcctttttt
    23161 tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca agttttttac
    23221 cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt tttcctctcc
    23281 tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt cattattctg
    23341 gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg attcccgcac
    23401 ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta aataaaagag
    23461 cagagtctac accataggca tctgccgttg cacctgtata aaataatccc atgaatccta
    23521 acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag ctgatcgtta
    23581 ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag ccaacaagta
    23641 ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat ggcgttgttg
    23701 gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc accatgccat
    23761 cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt gttaaactaa
    23821 tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg atgattgcta
    23881 acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt ttttcacccc
    23941 tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag accatccgaa
    24001 tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca agacttgttt
    24061 gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt ctgtcaaacg
    24121 taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc tggaatacca
    24181 gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc accgcctact
    24241 ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt tgattctttc
    24301 gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac aagaatatct
    24361 gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat atctttcact
    24421 tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt gaatacatca
    24481 ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg tagctctctt
    24541 acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg tgatttaaca
    24601 attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta cgcttcttta
    24661 cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga aacgattttg
    24721 aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc gattgaccag
    24781 ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa agccattgca
    24841 cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat agcttcccag
    24901 attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat ttgaccaaag
    24961 tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg atttgcatgc
    25021 tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg gtttttgatg
    25081 atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc actcattggt
    25141 gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac atctgtaaca
    25201 actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac ttgcccaccc
    25261 atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc tgcgacttca
    25321 ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc ttgttctgtg
    25381 tatccaatga atttactttc tactttgata tctgtcaata aagcagattg tacccccatt
    25441 gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat ttctttttca
    25501 aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt taattctacc
    25561 gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt cgtcccgttt
    25621 tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt gattgtttca
    25681 ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg ttgaagtact
    25741 tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata taagaatgct
    25801 tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg gcgtaagaca
    25861 taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga aagggcgcga
    25921 atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc atattttact
    25981 tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt ctcaaagttc
    26041 gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt atcaatgttt
    26101 ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga aaatactaag
    26161 ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg atcgtcttct
    26221 gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg accacatggg
    26281 cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag tggcaggcca
    26341 acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt tacataaagt
    26401 ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc ccagtggatg
    26461 gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa catcgtatgg
    26521 tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt ttgggcattt
    26581 gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa tgttgcaaca
    26641 ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc actaggttca
    26701 atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg tgcactactt
    26761 aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca ccatcacagc
    26821 caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat tacctcttaa
    26881 actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg agcaatttga
    26941 ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa aaaacaaatc
    27001 atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa tatctcaatt
    27061 tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag aataaaggaa
    27121 tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata gttggagcgg
    27181 atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta ttagaacaag
    27241 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
    27301 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
    27361 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
    27421 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
    27481 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
    27541 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
    27601 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
    27661 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
    27721 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
    27781 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
    27841 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
    27901 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
    27961 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
    28021 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
    28081 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
    28141 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
    28201 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
    28261 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
    28321 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
    28381 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
    28441 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
    28501 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
    28561 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
    28621 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
    28681 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
    28741 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
    28801 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
    28861 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
    28921 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
    28981 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
    29041 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
    29101 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
    29161 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
    29221 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
    29281 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
    29341 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
    29401 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
    29461 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
    29521 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
    29581 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
    29641 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
    29701 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
    29761 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
    29821 ggaaataata tcttaaataa atgccaaacg gtatgagtaa taaaacatta aaagctggtt
    29881 ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac aagaccaata
    29941 ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc tttggccctg
    30001 tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa tacgcacaca
    30061 ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat gttccatact
    30121 ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat ggaaaaacaa
    30181 tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt gattctttgt
    30241 atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct attatttctc
    30301 atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct gaatatgtac
    30361 tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc ttatgtaaga
    30421 aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg tttcatgcta
    30481 gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat gactccacat
    30541 gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat tttcatttct
    30601 ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca gttacgcaaa
    30661 tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt gctatttcat
    30721 cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca tttcttccac
    30781 gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct aataaaattc
    30841 gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc aagtgtcaat
    30901 actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac gataaaagtc
    30961 cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta tggtcgaaac
    31021 aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt ataaaaaatc
    31081 caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat catcatgcca
    31141 atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt atcatgaagt
    31201 ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata agggagacgc
    31261 attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat accaactgtg
    31321 accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc cgcattgctg
    31381 tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac taataaataa
    31441 acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt cgccggcttt
    31501 ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa catcgttgct
    31561 agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg gatcggaaac
    31621 agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac aaacaaagga
    31681 ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga acctaaaaaa
    31741 ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat tgactgggaa
    31801 agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc tacatttgct
    31861 ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt tccgtaaatc
    31921 cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag ttcagctcct
    31981 gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat atgcaaactg
    32041 gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc accgatcact
    32101 gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa ttcattttct
    32161 atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc cctttcatac
    32221 catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt tataccgtct
    32281 ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct aagtcgtatt
    32341 tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt ggccaagatt
    32401 ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct ggtatgattt
    32461 tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata ttgaaggcat
    32521 ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca tcgatcgcat
    32581 ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg tactttcctt
    32641 ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct cctggcaagg
    32701 ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca tatttcacat
    32761 aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg gttgtaggac
    32821 gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac gcgccgattg
    32881 aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca ttgaccagcc
    32941 cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct gacaaaagag
    33001 cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc attttttcgt
    33061 aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca ggcatgcgta
    33121 tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg atcaactctg
    33181 aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc ctccctgatc
    33241 ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc tctaatttta
    33301 aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta ttttttgata
    33361 aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca tatttctgat
    33421 cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa tcccatgttc
    33481 gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga ctggagtaat
    33541 aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt taaaataatt
    33601 agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag taatcgaagc
    33661 caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg gccgacttct
    33721 agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa caacacctat
    33781 agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa aatgcttgac
    33841 ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt aggtgatcag
    33901 tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc catccatgga
    33961 gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta tcgcttcctt
    34021 tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt ttgctccgga
    34081 taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga tagcatcttc
    34141 ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt gaaaggcgat
    34201 tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat ggattttcag
    34261 cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg ttgcttcaat
    34321 acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg atgctagatt
    34381 gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta gtatgtcgat
    34441 cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat acattgttag
    34501 ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac ataccaaaca
    34561 tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg cttacactga
    34621 ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt caaatgactt
    34681 atatagtatg aacaattacc ttttagataa tgatctattt tactctacct gggcagataa
    34741 atacaacaca accattgata gaagcccttt cgttaaaatc aattgtattc tgaatattta
    34801 atttatttat tgcatagacc aataaaaata cataaaattt ccatcttcgt ttatcagcaa
    34861 tttttaattt tactctctga atatttctta aaagttttac ttgttgaatt cgaaaaaagg
    34921 actgtgacaa aagtcttgtc acagtcctat tcttccgaat a
//
LOCUS       DAZUKT010000022        34743 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000022 DAZUKT010000000
VERSION     DAZUKT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.022"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG816_001047"
     tRNA            8..81
                     /locus_tag="ACG816_001047"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACG816_001048"
     tRNA            101..173
                     /locus_tag="ACG816_001048"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACG816_001049"
     tRNA            201..274
                     /locus_tag="ACG816_001049"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACG816_001050"
     tRNA            288..361
                     /locus_tag="ACG816_001050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACG816_001051"
     tRNA            385..474
                     /locus_tag="ACG816_001051"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACG816_001052"
     tRNA            487..560
                     /locus_tag="ACG816_001052"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACG816_001053"
     tRNA            579..654
                     /locus_tag="ACG816_001053"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACG816_001054"
     tRNA            656..726
                     /locus_tag="ACG816_001054"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACG816_001055"
     tRNA            740..813
                     /locus_tag="ACG816_001055"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACG816_001056"
     tRNA            838..925
                     /locus_tag="ACG816_001056"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACG816_001057"
     tRNA            947..1018
                     /locus_tag="ACG816_001057"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACG816_001058"
     tRNA            1041..1130
                     /locus_tag="ACG816_001058"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACG816_001059"
     tRNA            1143..1216
                     /locus_tag="ACG816_001059"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACG816_001060"
     tRNA            1235..1307
                     /locus_tag="ACG816_001060"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACG816_001061"
     tRNA            1311..1383
                     /locus_tag="ACG816_001061"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACG816_001062"
     tRNA            1388..1458
                     /locus_tag="ACG816_001062"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACG816_001063"
     tRNA            1471..1544
                     /locus_tag="ACG816_001063"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACG816_001064"
     tRNA            1556..1627
                     /locus_tag="ACG816_001064"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACG816_001065"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACG816_001065"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4919956.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACG816_001066"
     CDS             3216..4688
                     /locus_tag="ACG816_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4919957.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACG816_001067"
     CDS             4771..5559
                     /locus_tag="ACG816_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4919958.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACG816_001068"
     CDS             complement(5626..6294)
                     /locus_tag="ACG816_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4919959.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACG816_001069"
     CDS             6409..8121
                     /locus_tag="ACG816_001069"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919960.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="ACG816_001070"
     CDS             8124..9896
                     /locus_tag="ACG816_001070"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4919961.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(9978..11099)
                     /locus_tag="ACG816_001071"
     CDS             complement(9978..11099)
                     /locus_tag="ACG816_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4919962.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(11171..12451)
                     /locus_tag="ACG816_001072"
     CDS             complement(11171..12451)
                     /locus_tag="ACG816_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFS4919963.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(12542..12994)
                     /locus_tag="ACG816_001073"
     CDS             complement(12542..12994)
                     /locus_tag="ACG816_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4919964.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(13007..13417)
                     /locus_tag="ACG816_001074"
     CDS             complement(13007..13417)
                     /locus_tag="ACG816_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4919965.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            13692..13904
                     /locus_tag="ACG816_001075"
     CDS             13692..13904
                     /locus_tag="ACG816_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFS4919966.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            13907..14119
                     /locus_tag="ACG816_001076"
     CDS             13907..14119
                     /locus_tag="ACG816_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919967.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            14259..14414
                     /locus_tag="ACG816_001077"
     CDS             14259..14414
                     /locus_tag="ACG816_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919968.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(14411..14635)
                     /locus_tag="ACG816_001078"
     CDS             complement(14411..14635)
                     /locus_tag="ACG816_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4919969.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            14713..14901
                     /locus_tag="ACG816_001079"
     CDS             14713..14901
                     /locus_tag="ACG816_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919970.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            14898..15071
                     /locus_tag="ACG816_001080"
     CDS             14898..15071
                     /locus_tag="ACG816_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919971.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            15040..15168
                     /locus_tag="ACG816_001081"
     CDS             15040..15168
                     /locus_tag="ACG816_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919972.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            15227..15562
                     /locus_tag="ACG816_001082"
     CDS             15227..15562
                     /locus_tag="ACG816_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919973.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            15795..16736
                     /locus_tag="ACG816_001083"
     CDS             15795..16736
                     /locus_tag="ACG816_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFS4919974.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            16738..17628
                     /locus_tag="ACG816_001084"
     CDS             16738..17628
                     /locus_tag="ACG816_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFS4919975.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            17671..18471
                     /locus_tag="ACG816_001085"
     CDS             17671..18471
                     /locus_tag="ACG816_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4919976.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            18486..19337
                     /locus_tag="ACG816_001086"
     CDS             18486..19337
                     /locus_tag="ACG816_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4919977.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            19403..19537
                     /locus_tag="ACG816_001087"
     CDS             19403..19537
                     /locus_tag="ACG816_001087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919978.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            19538..19999
                     /locus_tag="ACG816_001088"
     CDS             19538..19999
                     /locus_tag="ACG816_001088"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4919979.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            20025..20477
                     /locus_tag="ACG816_001089"
     CDS             20025..20477
                     /locus_tag="ACG816_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919980.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            20583..20777
                     /locus_tag="ACG816_001090"
     CDS             20583..20777
                     /locus_tag="ACG816_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919981.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            20808..21101
                     /locus_tag="ACG816_001091"
     CDS             20808..21101
                     /locus_tag="ACG816_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919982.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            21032..21571
                     /locus_tag="ACG816_001092"
     CDS             21032..21571
                     /locus_tag="ACG816_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4919983.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            21646..22113
                     /locus_tag="ACG816_001093"
     CDS             21646..22113
                     /locus_tag="ACG816_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFS4919984.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            22860..23090
                     /locus_tag="ACG816_001094"
     CDS             22860..23090
                     /locus_tag="ACG816_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919985.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            23249..24082
                     /gene="terS"
                     /locus_tag="ACG816_001095"
     CDS             23249..24082
                     /gene="terS"
                     /locus_tag="ACG816_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFS4919986.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            24075..25490
                     /locus_tag="ACG816_001096"
     CDS             24075..25490
                     /locus_tag="ACG816_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4919987.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            25502..27031
                     /locus_tag="ACG816_001097"
     CDS             25502..27031
                     /locus_tag="ACG816_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4919988.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            26949..28001
                     /locus_tag="ACG816_001098"
     CDS             26949..28001
                     /locus_tag="ACG816_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFS4919989.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            28003..28320
                     /locus_tag="ACG816_001099"
     CDS             28003..28320
                     /locus_tag="ACG816_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919990.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            28447..29070
                     /locus_tag="ACG816_001100"
     CDS             28447..29070
                     /locus_tag="ACG816_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFS4919991.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            29085..29975
                     /locus_tag="ACG816_001101"
     CDS             29085..29975
                     /locus_tag="ACG816_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFS4919992.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            29998..30213
                     /locus_tag="ACG816_001102"
     CDS             29998..30213
                     /locus_tag="ACG816_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919993.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            30225..30566
                     /locus_tag="ACG816_001103"
     CDS             30225..30566
                     /locus_tag="ACG816_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919994.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            30566..30937
                     /locus_tag="ACG816_001104"
     CDS             30566..30937
                     /locus_tag="ACG816_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919995.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            30930..31325
                     /locus_tag="ACG816_001105"
     CDS             30930..31325
                     /locus_tag="ACG816_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFS4919996.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            31327..31704
                     /locus_tag="ACG816_001106"
     CDS             31327..31704
                     /locus_tag="ACG816_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFS4919997.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            31705..32313
                     /locus_tag="ACG816_001107"
     CDS             31705..32313
                     /locus_tag="ACG816_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS4919998.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            32313..32654
                     /locus_tag="ACG816_001108"
     CDS             32313..32654
                     /locus_tag="ACG816_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4919999.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            32896..>34743
                     /locus_tag="ACG816_001109"
     CDS             32896..>34743
                     /locus_tag="ACG816_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4920000.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTN"
BASE COUNT        11644 a         5418 c         7162 g        10519 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtatct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag ttgattttgc
    10021 atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc atgtcctaca
    10081 cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat gtgggaatgc
    10141 cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac tgctcttaaa
    10201 gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa aatgtattta
    10261 tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat ttctttagca
    10321 ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt tttaattttt
    10381 aactcgtaat cataagtacc agaaaccgta attttattac catcataatc agattcagtc
    10441 aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt aattaaagaa
    10501 taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac ttcttctttt
    10561 gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt tcttgctaaa
    10621 gaaaccccat ttacgggatt atattttaat ccatatcgac ggttggcgaa tttaaaaatc
    10681 attcctgtaa aagtttttaa atgtattaca taattatatg ataaattttt ttcaaaatag
    10741 tagtaatcta gcatatcttg tataagatta ctattaacgt tttttacaag tacatcatct
    10801 tttatatatt ttgaaattaa tgatagataa acctgagttg ttcgatagga actttctttt
    10861 acagttggct tgtatttttc atcccattta gtttttaatt ctccaaaagt tagtggtaat
    10921 tcttcatttc gttttatttt ttctgcgatt ttttcattca aaagtaagtt agcttgattc
    10981 caagcttggc gtgaattgct agataaagta acggacactt ttctcttttt ttcagttaag
    11041 ggatccgtat aacgttcggt gtacttaaat tttttgtcat ttattttttc taaccacatt
    11101 atttctattc ctttcgaata tacgttccta ttaattgtta aaagaaaaag cccgaaggct
    11161 ttttatatat ttaatctttt gacaattcta aagatcgtct atattgttca gctttagcta
    11221 attttgtaaa tgttactgtt ccattataat tttctaaaac aactttctct atttcgtcta
    11281 gtgaaacttt gaaaaattct tttctttgat taattttatt aacttcttgt tttctaaaat
    11341 gattatgcaa tttagtttcc aaagacggag cgtcctctga aaaaatcatc gcatgaacgt
    11401 caaattcaaa tggaaccgat gcactactca attcttttat tctatccata ggttctaatc
    11461 ttctagtcat accaatttta tatatgtctt caccaaaaga cccaatatta ctaattacat
    11521 acacataacc ggctctagta tttaattctc tttgtaatac attttcacgt ataacttcta
    11581 attcttttaa tttttcttcc agttcttgaa ttttatttgc atataattct ttttccacat
    11641 ctgcattagc tttttgcata tatgcgatta atttggaaat ttcagaattg aattgacgtt
    11701 gttctttatc aatacgtttc ttttcacgat caatttctct acgaactttt tcctcttcta
    11761 tcatctgttc ttttattgct ttttgttgtt ctttttcttc ggtaattttc actaaataag
    11821 agtaatgaag ttctagttct tgaagtttaa actctaaaag acccttcctt aattcagcat
    11881 tgtcattctt gaataaatta ttaattttat caaatatttt tatgattttt tccctagtcg
    11941 agtctatatt agaaatagtt agtttattga gtaatccttt cacttctgaa tcaaatggaa
    12001 tgagcatttt tttctttaaa ttatttttct ctcgtttatt taaaataaga gggacagaaa
    12061 cagcatcgtc aataatatat tccttttctt ttaattttaa caaagaaagc ttatttttta
    12121 tttcagaaga tgaagtttct tctatatttg aaacgataag tgaagaatag acatttttta
    12181 atgtttcatc atacttttga tttatttcat catttaaatc ttctttctct ttttttaata
    12241 aaataatatc attttttaac atagtaattt gtttatttaa atctgatagt gtttcttcct
    12301 tacttaatat aaatctttct ctatcatgta atttatttag tttttcatca tttttttgtt
    12361 ctaattcata agcttctttt tttattgtgt tagctttcga cagtttagtg attcctataa
    12421 ttaataaaat aagtccaatc aataataaca ttcaaaactc ctcatttctt tgatataatt
    12481 attgtagagt ttactcagaa atgaggaaag agtccatgtg aaagcatggg cttttttatt
    12541 tttaatgttg taatactaaa tgttcaagat tatgaggaag ccctaaatat tcaataattt
    12601 ggaactgact catatcagat aaatcttgtt catctattgt tctagttaac atttcaattg
    12661 caaacatatt agcttcagct tctataccat aaatcataca actaaggttg tttgctctaa
    12721 aagctgctgt gcaaatacct ttatggagtc taacgtgggc tatttcatgc cataatacaa
    12781 atcttttcat agaaggtgat aggttttgat taattaatat cacataacat cttttattgt
    12841 gtatcgtttg tcctaatgtt ttattcccta aatcagtttc aattaaatct atatcagaat
    12901 atttgattat tttatatgga ttaatcgcat cagatccaaa ctctacaaat aatttttcta
    12961 tttcttctaa tatccagtat ttgtttaaat gcataccaaa gcccacctat tgacgatatt
    13021 ttttaggagt gaattttttc tttgctttaa ctttggttaa ggcaagtgca agttgaatgg
    13081 catcgtataa tttttcctta tcttcatcgt ctaaaggttc accataataa tttgtttcag
    13141 catctgaatt caagccttca agtaattttt cagcttgtaa agcaatatct tgtttatctt
    13201 tttctgttaa atcataataa tggcgtttat cagtacgtcc taaaagataa tcagttgata
    13261 cattaaagta atctgcaact ttttgtattt tatctccaga tggagtactt ttattccatc
    13321 tagacatgct tccatttccg aaatttaact ttctttcaag ttctgccaac gtcattgaac
    13381 gttcttttaa taagattttt atacggtcta atagcatagg atatgcattc ctttcaaaat
    13441 accgacaaaa aaagtatttt tctgcaaatt atctatttac aaatagagaa tttgcagtta
    13501 tactagtttt gtaagcaaga tatttacggc atacaaagac taaaagcaat aaactaatag
    13561 tctccccaga catcagtaag ctcgcttttt gtgcgctgta ttttgtatgc ttacataata
    13621 gcatatttgc agtttttgtc aaacttatta gacaaaaata ttagaaaata ttcgataagg
    13681 gggtatgaag catgacttta aaacagcaag tattaattgc tttagtaaag aaaggttgga
    13741 gtcaaagaga gttagctcga cgaatgggta tatcaattac ttatttacga gatatcttca
    13801 tcgaaaaaag aaaacctaag gaacgtttaa aacaaatcga agaacttttg gatatcaagt
    13861 tagaagtaga ttctaatgat cagccatcag aacaggaggc gtaaaaatgg tacaacgttt
    13921 gagcaaagcg gagtttaaag agaaatataa gtattctgaa agtacttatc aaagaagaat
    13981 gaaggaattt aaaaaatcac gttttagtga ggggtataac gcagttactt ccaaagaaat
    14041 aacaattgat gtcgatttat atgaacagtt tagaacatgg cgttctaaaa atcgatttag
    14101 agttagagct gtttgttaat agctaaaaaa tctaaattca ccacttctga aaagaggagt
    14161 tgctgctctc ctttcaagtt tgccaaagtc cacgtttttt cattttggca actcctcttc
    14221 ttggaggtgg tgacggcgag agacgaagga gggatgacat gaaggaagaa tcaaaaatag
    14281 ggattttgaa tgagaacaat tacacaagat taattattga aacagaagaa gggaaaaaag
    14341 tagcagaagt tacattgacc gatgcaactc ctgctgacgg ttatcgtatt cgattaactc
    14401 ctaaatatga ttaacctttt ggtgggtgtg gatcgtttcc gtgactatca cggctttgaa
    14461 tttttccatc tctgccatga ataaatagct cagatccttg atttttagaa atctctctag
    14521 cgatttttac tgcttctgat tttgtattag tatgtgcagt cgctttagaa tttccagcac
    14581 ctttgacatt ccaaccacca ttggaattag gtacaacatg ttgatttttt cccatagttt
    14641 tcacctccat ttctatagaa atattttatc aaatagaaat ggagaaattc aataaacgaa
    14701 aggagaagtg atatggcata taccttgcaa caagaacatc aaattctcgg tttgattaaa
    14761 caacgcagga aacaattaca agatgaccgt gcagcgctta gaaaagccga tgagctatca
    14821 gataggcaag ctgaactaat tgcttctgaa cttgaggatt tgagaatgct agaaataaaa
    14881 aatagggaga ttagattatg aagaagacag acacactttt tataggattc attttggggt
    14941 tattagtgat tgtagcgcac caaagtatta tcgggggaag cttgttcgca gcattgatgg
    15001 ttttaatcaa tctgcttgat tcaaaagaaa ggagcaacta tggcacgaga agaagcgcta
    15061 aaaatcggta aagtgattgc tgataatcgg tggggaaata gccgccctat tattttaagc
    15121 aagcaacata tcgaaaagca aaaagcatgg caacaaataa aaaagtgact ctgccagcca
    15181 gcaaagagtc acatacaaat ttataaaact aggagaatta tatcacatga ataagaaaat
    15241 tgaaaatctt atcgaagaat tgaaacgtga atgccaaaag caaggtgttt ctattatttg
    15301 tacagcccaa aaagaagggg aacttaaaag tctcgtttat ggtgagacaa cagaaatttt
    15361 actttgtctt gctatgcaag aagaacattt agatgaaaat ctcccattgt cagcacatat
    15421 tatgcgtaga attgctgtgg atgcttacga acaagcgaaa aatgaagaag aaaatcaacc
    15481 ttcaaatcat acttttgtta ttaataacaa ggaagattta gctgatgtga tgactcgtat
    15541 tctcaagggg gaatttcaat gatgaatgaa aaaacaattg atctactcat tattttattt
    15601 ttcacgttag tgattgtagc gaaaagttat gtaatctttg gcttgttatt cgcaattacc
    15661 atgcttgtgc tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg agttagcaaa
    15721 tcttgacaac tatttaactg atcctgatta tgctaaacca ccatatgaag caccaattga
    15781 tgaggaggat gaagatgagt aaatctacct tagaaatgag ccatcaagaa tggcttgaag
    15841 accgtaagaa aggcatcgga ggttcggatg ttggaacaat tttaggattg aataaatgga
    15901 aatctcctta tcaactatgg ctagaaaaaa caggacaagt agtacttgaa gaatcaggaa
    15961 gtgagcccgc ttattggggt aatgttttag aagaagtggt tgctaaagag tttcaggaac
    16021 gtacaggcaa aaaagtacgc agaagaaacc aagtatttga acatccatta catccatttt
    16081 taagagcaaa tattgatcgg gaagtagtgg gagaaaatgc cattctggaa tgcaaaacag
    16141 ccaatcaatt tctcggtaaa gaatgggaag gcgaagaggt accgcttagc tatctctgtc
    16201 aagttcaaca ttacatgaac gttctaaaca aagactattg ttacatcgct gtcttaatcg
    16261 gtggtcaaaa atttatctgg aagcggattg aacgagatca agagctgatc gatacaatca
    16321 ctgaacaatt agtagagttt tgggaaacaa acgttcttgg aggtatcgag ccaattattg
    16381 atggtagtca agcgacagct gatttcttga aagagaagta tgcagatgtt gaagacgttc
    16441 aaacgacgtt accaattcat ttcgatgaac tagttgaaca gaaaaacgaa ctcaaacgga
    16501 ctaagaaaga aattgaatca gctatccgtc aagtagacaa cgagattatc agcgagctag
    16561 gtaaacgtga agccagtatc ggtatcactc aaaaaaacat catcagttgg aaatttgtcc
    16621 gtacgagacg tatgaactcg aagaaactag cagagaaata tccagatgtc gcaaatgatg
    16681 aagagattta taacgttact gaatcaagaa ggctaaccga aagggagatc aaataatatg
    16741 gcaacaaatg aatcgttaaa aaatcaattg gcagcaaagc cacagaaaca agttgcacca
    16801 ggtcaattag gacttaaagc tttgatgaat acaccaacaa tgagaaagaa gttcgaagag
    16861 gtgcttcatg acaatgctaa tgcttttatg tcgaatgtta tgactcttgt atctaatgac
    16921 agttatcttg cagatagtga accgatgtct atcatgagtg gtgcgttaac tgctgcaaca
    16981 ttaaatcttg ggctagataa gaatttaggt tatgcatatt tagttccatt caatagtaaa
    17041 aacaagcaaa caggaaaatg ggaaaagaaa gctcaattca tgcttggcta taaaggatat
    17101 atccaattag cccaacgatc aggtaaatac aaagcattaa atgtgatcga agtttacgaa
    17161 ggagaactaa aaagctggaa ccgactgaca gaagagtttg agtttgatcc aaatggtaga
    17221 acgtctgatg aagtcattgg atatgttggc tatttcgagt tactgaatgg attcaaaaaa
    17281 actgtctatt ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa taaagatcgc
    17341 gataagacaa agttaagtgg tgtgtgggca tctgattaca atgcaatggc acgaaaaact
    17401 gttttgagaa atcttctttc taaatgggga atcttgtcca tcgaaatgca agaagccacc
    17461 acatcggatg agagagtcca aagggttcaa gaagacggca gcattattgc tgaaacagaa
    17521 gttgaagaag atattcctga aagaaaagaa gcagaggtta tatctgaaga aaatgaagat
    17581 gtacaaactg gattatttga tgcatctaat ccgccgttaa acaaataatg agggagcttt
    17641 ctcccttgct tttctagagg agaaatacga atgaacacag gatatgtcaa attacatcga
    17701 aaagtgatga attcattcgt ttggaccaat ccttatatgt ataaattatg gaacttgtgt
    17761 ttgatgaaag cgagtcatga gaatcgcaag atacttttta atggaaaaga gatacaagtg
    17821 aacagcggag aattcgtcac agggcgcgat gctatcacat ctgagatgaa cgaaggcgtg
    17881 aaacgtgaac atcaagtgaa cagcggttct gtatggagat ggctaaaaca atttgaaaaa
    17941 aacggaatgt tgaacatcaa atcaactacg aaatacagcg ttatatcaat aaaaaactgg
    18001 tctttgtacc aaagcagtga acaacaaatg aacatcaaac gcacaacaag tgaacaacaa
    18061 gtgcacacaa acaagaatga aaagaattat aagaatgaaa agaataataa aacatcatcg
    18121 tcgcaaccac gcaaaaagcg tgtttacgac accgactcag tttactacat tctcgcggag
    18181 gagttattca aacagatttg tcagaatcag gaaatcaaaa agccgaatct gcaaagttgg
    18241 gcagatgaca ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca ggtacgggga
    18301 atgattgaat ggagtcagca caatgtgttt tgggcatcga atattttatc tgctaaaaaa
    18361 ttgcgagaaa aatacgacac aatggcagcg caagcaaatc gtgattataa aacaaaacaa
    18421 actaaaacgc ttgaatacga gaaatttagc acagatgagt tgcctatttg agaggaggcg
    18481 taggcatgga aactgttggg gaaatcatgg aaaagctgat acagaaagtg cttgtccaac
    18541 gtggcgaatg tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag aaccctgatg
    18601 gctcagaacg ttgtaagcca acctgtatgc aatgtgggta taaagcattg cgagttcagg
    18661 aagacttaca aacagaacga atttacaacg agagcctgaa agcaagagca atcaattttt
    18721 tcaaaggtgg ttctgttgtg cctaatcaag cgttgtttga ttgcacattg cagaattatc
    18781 aaattgtcga tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt gttaattcag
    18841 tcttgttagg aaatccaagt caccttgttt taactggaaa acaaggaaca ggtaaaagtc
    18901 acctagcgat ggcagcggct tgggaagtct tgaagcgatc aaactacgat aaaaaaatct
    18961 tatttatcgg gttacaggaa atgctggatc aaatcaaatt ttcgtacaac aatcctgaac
    19021 tcagaaaaac gattgaggga tcgttgattg cagatatcaa aacagcggat ttagtcatca
    19081 ttgatgatat cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg tttggcataa
    19141 acacgctaaa ttcgttcttg gatgcacgac agaacctagc aacgattatc acaacgaact
    19201 tgcttggtga agaactgaaa aaagcttacg gtacgagaac aatatcaaga atgtttgtca
    19261 actctgatgg atttacgatg gtattttctc aaacagcaga caagcgcata aaaccagtca
    19321 aaggagcgat cgcatgaatt tgactaaaca agaaaaaaga atctgtcgtc agataatgtt
    19381 gcaaattata ggtgatcgtg acatggacga attatctcaa caagagagtc gaatggttcg
    19441 gaagataagt aagctaatta tttatcggga tacagaaaga tttctcactg ttttaggtaa
    19501 cgtctacgag aatccagagt tattggaggt agagtaattg ccaaaaatat tagatgcatg
    19561 ttgcggtagt agattgttct ggtttgataa gaacaattct gacgtaacat ttatggattg
    19621 tcgtcagcag tatgaagaat tatcaacagg tcatgtgatc aatgttgatc cagatgtggt
    19681 cgcagacttt cgcgatatgc cttttgaaga tgatgtgttc gatatggttg tatttgaccc
    19741 gccacactta agacatgtcg gcgaaaactc atggcttgcc aaaaaatatg gaaaattaga
    19801 cgaactttgg ccagaagaca tcaggcaagg ttttgcagag tgtatgcgag ttttacgacc
    19861 ttcaggaaca ttaattttta agtggaacga ggaacagata ccactttctg atgtgttaga
    19921 ggctattgga gaacagccat tgtttggtaa taaacgaagc aaaacacatt ggcttgtgtt
    19981 tatgaaaaaa ttcagctaac ggaggaaaca aaggaggaaa agtaatgaag attgtattaa
    20041 ataaatgttt tggtggcttt gggttgagcc atgaagcaaa aatggaaatt tttaaaagaa
    20101 aaaatattga agtattccca tacatcaata actttagcta tgactcagat gatgaataca
    20161 cgagatacac aggccaaaag ctaggttcga tggattttat ctactatttc aaaaaagacc
    20221 cgaaaatcga caaagttaca ggaatttata gcgaaattga ccgactgtac ggaattgctg
    20281 atgattccag tttttcaagt gattcaaaca gaggtgataa ggacctcgtt gctgttgtcg
    20341 aaaaactagg aagtgaggcc agtggaccat atgcaagtct taaagtcgtt gatatacccg
    20401 atggggctga gtgggaaata agtgattatg acggcgttga aactgcacat tatggttttc
    20461 aaacaggtag tatttagtcc actaatgaca actaaagagg aggcagaagg atgagtgaag
    20521 atgaatactt tgaacagtag tctattaacg acagagattt ttaactggaa aggggtgtag
    20581 tcatggacgc atcggaagta gtaaataaaa ttaatagtct caaagaggtt ttcggagacg
    20641 tcgaagtagt gataaacgca caaggctatg acaacgaatt ttttaagcag attaccgaca
    20701 ttagcattca gcaaggaatg gaagacgaag acggaaactt tatcgatgaa gtagccattt
    20761 tagtgacttg cgagtaagta attgacagct aaagaggagg aagcggaatg aaactaaaag
    20821 acggatttta ctccagcggc cacggcatcg gcggtttaat gctagatatg ccgacaaaga
    20881 atcctaaaac acgtaagaaa ccaaaattca aagtcggtga catggttcgc tgcgaagcag
    20941 aaggattcat ctatccattt cgtggatatg tagaacacgt ctataatcac tcagcaatca
    21001 ttcgcattga aaacacgatg gaatgtgata agtggttagc gaaaagcaaa gagaatttag
    21061 cagtggtgag attggtggac atggaagtta taaaatctta attttgcttt tttacaacta
    21121 tcctacaaca atgagcgtta ttccacaact ggaaggagaa gaggcaattg tgaacatttt
    21181 agagaatata gatattaagc aaacaaggaa aaatgctaga cgattactaa aaagatacag
    21241 aaatttagaa cgtttggtcg gaccagtgaa aatagacttt tccgtgatga ctgttaccaa
    21301 aaatttgaaa ttcacaattg acagtcaaaa cgaagaaatc attgaagcga taagtactcg
    21361 agattcggtt atcgaagcat taacacggct aagtagaatc catttccagg tactttatta
    21421 tagttattgt tttcctaata agatgtcaat gtatcaaata ggagaaaaat taggatattc
    21481 cgatagaact atcgagagaa tgaaggcagt agctttggtt gaatttgctg aggcgtataa
    21541 atcaggagaa ctcatttcac gtacaaaata aaaaaagtcg gattcctccg actatgaata
    21601 atatttccga cataagtatt atatcataat tgggggaatc agaggatggt acttttcgat
    21661 gtaaagaaat atgagacacc aagcgcgaaa gatgttgata tggaacgcac aaaacataat
    21721 gtcgctgtgt ttctttcagc atatctatca gctagatgta gagtagggca acatcgagaa
    21781 ccaaaagtga cagcatctta ttccttggtt ccaccttcta cagctgatca tatatttgaa
    21841 gcagaaagaa tgctgatcga taaagaagaa gcacaggaag aatttgagta tctgcataaa
    21901 ttgtttgttc gaggctattc agcgatacag catccgcata agcctgatgt gactgaaagg
    21961 cgcaagaaga tattctatga tcgttatatc aatggtctgc ccatttatgt aactgctcaa
    22021 aggaataata ctagcgaaga atcggttaaa gtagaatcaa acagaattat catccaattt
    22081 gcttcatcgt tagaactggt tgctttcaag tagccagttt ttacactttt tatacccttt
    22141 tattaccaat ttggtttcca ttttatacct tttttatacc aatcacttac ctattcaacg
    22201 ttgtattatg atagtgtcga aaaattagga aacaggatcg acaaaataaa atgtaaggga
    22261 ggaaatctcc ctcatcgttt aattaagctt cgataggcag caacggaaat attaagaata
    22321 aggatgtgaa tttcaactcc ttctaaattg ttcttattat ctatcatccg ttgctgtcta
    22381 ttgttttttt aattattcac acgataacta aaggtaagtg aagagaaatg attccattaa
    22441 taatttcaat ttttgcgctt tgtcttaatg tatatatgat tggatttaaa aatgggcaaa
    22501 ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga aagggtagac
    22561 gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat tccttgccag
    22621 tgactttggc aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa tttcgtacgc
    22681 gttttgtgca tcgtgtaggt tgctattaca taactggtta ggttagattg agatttggga
    22741 ttcggtacaa atgaatcgtc aaatgtctca agcacaggat cggaaacgtc cctgcctgtg
    22801 cattacatat tagatcactc tttgagtggt ctttttcttt acataaagga ggctacataa
    22861 tgagaaacta ctggtatgta tcgctaacta atgaatatcc tcgaaccatt gatgattgtt
    22921 cagtgcgtgt tgtgcgttct gtacaaatca aagggaagta ctctattgtc gaaatgggga
    22981 gagaagcaga accatgtgag attgatgcgt gcaagctcgt ttattgcggt catggtttct
    23041 atgatgaacc aaacattcaa aataatatta acaagaattt gagggattag aatgcaagaa
    23101 atggcttaaa aatctccaat caggacaaat caagttagac aaagtttccg atttaaagat
    23161 attgattgaa gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa aactcaacct
    23221 aaacagaatg tgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa gacgtgatga
    23281 agccaaaaga atttggttag aatccaacgg agaaaagcag ttaaaggaaa ttgcatctga
    23341 attaaatgtt tcagattctc aggttagaaa atggaaatcg caagacaaat ggagcgctga
    23401 attgattagt aacgttacca atggcaaaag taacgttact aatcaaggtg gcgctcctat
    23461 tggtaatcaa aatgctaaag gtaacaaagg aaatagccga gcttctccgc cagtgggtaa
    23521 taaaaacgct ttgaaaacag gtgagtatga aacaatattt tttgacacgt taaccgataa
    23581 tgagaaggac atctattcta gtttgaatga tgatccttct tttgctttgt ccgaagaaat
    23641 acgacttctt aaaataagac aacttcgaat gatgaaaaga atccaacaag ctgaagctgg
    23701 actaaatgat gaagaagtcg aacgattgca gcagctaaga aagattaaaa ctccgattga
    23761 aaaaaatggt aaaaagctag aaatcaagcg tgaggttatg caagatgtgc agattagcag
    23821 aaaaaaacat cgcaaaattg atgatattct ttcaattgaa gattcattga ctcggattag
    23881 caaccagtta gctaaagcca tcaagcaaat gaatgaactt tatatgaatg aatacagaac
    23941 tgatttaatt aaagctcaga ctgataagat ccaagctgag acaaatgaaa ttggcggaaa
    24001 taattcaggt gaagaaatag aagaatggaa acaggcagtt ttaaatgccg caaacaaacg
    24061 ggcggtgaaa gaaaatgaat aatgaattta ttccttttgc tgatattggt tctgccattg
    24121 attattacta tgataaacca gtagcttttt gccaagatat tttgcatttg aatcctgatg
    24181 aatggcaaga aaatgtttta aatgatttag ctgaattttc aaaggtttct gttcgttctg
    24241 gtcaaggagt tggaaaaaca gcattagaag caggagcaat actttggttc ttaacgtgtc
    24301 gaccctacgc taaagtaata gcaacagctc cgacaatgaa gcaactttac gatgtacttt
    24361 gggcagaggt agctaaatgg ttaaatgata gcttgatcaa aaacttactg aagtggacaa
    24421 agaccaaaat ttatatggtt ggtgattcag agcgttggtt tgctacggct agaacagcga
    24481 ctaaaccaga aagtatgcaa ggttttcacg aggaccatat gttgattgtg gtagatgaag
    24541 cttctggtgt gtctgatcca attatggaag ctattcttgg tacgctatca ggttttgata
    24601 ataagctgtt gatgtgtgga aaccccaata atattgaagg tgtcttttat gattcccata
    24661 attcagaccg tgataaatac agagttcata aagtatcaag ctatgatagt aaacgtacaa
    24721 acaaagacaa tatagaaatg attcttaaaa aatatggaaa agaaagtgat gttgctcgtg
    24781 tccgtatttt tggagaattt ccgaaaggtg cgttggattc atttatcagt cttgaaacag
    24841 ttgaattggc tacagaaaaa caaattagtg attctttagt caataaaaca aaggttgctc
    24901 atattggtgt cgacgtagct cgatatggtg atgattctac gattctcttt cctagaattg
    24961 ctaccagggc attggagtat gagaagtatt caaaacgtag caccatggaa acaacaggat
    25021 atgtcatcaa catggccaag aatctaatga gtcaatatcc gagtattgat aaagtgatga
    25081 ttaaagtcga tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa gaacttatag
    25141 aagacaaaca ttatcctttt gaggtgtttg gagtgaataa cggttcaaca tcagaagacg
    25201 atttttacga taatttaggt actcaactat ggggaaacat caaggaaatg ttagaagaaa
    25261 atatgacagc aaatcttaac ggagaacagc ctgttattga attgccttct gatagttcgt
    25321 taatcaaaga attaagtact cgcaaattca aaatgacaag tagaagtcgt atacgtttag
    25381 aaagtaaaga tgatatgaaa aagcgaaata ttggtagtcc cgatattgct gacgcactgg
    25441 ctttagcgtt ttatgagcca ccaagtcact atcaatttat tcaattttag gaggtgagct
    25501 tttgattagt acagtattat ctttagagag atataagaag ctacgtgtta aatatgcaac
    25561 acaaattgaa gatggaatgt ttgatccgaa tggttttata gaagatatga aaccattttt
    25621 tgctgatcgc gaaagaaaat atctagctta tactagcgaa aagaatgaaa tagataatag
    25681 accaaagcct aacactgata ttgtaaaagt taataataaa cttcatgctg gtatgtattc
    25741 aattgtcgta gaccaagcag tcaatcattt tactggtata cctatcaaat gggattacga
    25801 tgtatcggaa caaaaaagaa cactcattca aagattaaaa gataaattct taaaaaatga
    25861 tatagaactt ccgacagttc cagaagcttt taacaaatta acaagcaatc ttgattccat
    25921 gagattcgca atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg gagtagcttt
    25981 ccgattgcta gagcctgtgg aagaagatga tggttggaag ttaagagcaa gtaatattga
    26041 accttggaga gcagaaagat atggaaatgc cggaatctat atcaaagaaa agtatgactc
    26101 ttaccagaaa aaattttttc aagaaatgaa agttatgaca agaaataaaa ttctgacata
    26161 tgcttgctat ggtgatttga attttgttac aagcggaacg ttcaaaaaaa ttgatgagac
    26221 cgataaccct ttggggacga ttaccttggc agaatttaaa aataatacga atcgttattg
    26281 tgattttgaa gtagctgagg aaattggaga tgcaattgat cgtgctttgt ctgatcaaca
    26341 aaacgaaatc gaacaattta agcttgcgta catgcttgtt actggcacaa caatgagcaa
    26401 aggcactgct aaagaaatga tgaatcagct agggattatc aacttaaaag cccctactgc
    26461 aaaagctgaa tatgtcacaa aaaatttagc aaaagatttc aatgaatatc atatggatct
    26521 attgaaaaaa cagttttaca ccatttgtaa agcaatcgat tttaacgatg aggtgtttaa
    26581 atcaaacagt tctggagaag ctcgcaagtg gcaaatcatt agcctagaag cgaaaacaaa
    26641 tactaaagag caatatttta gagaaggatt gaaagaatgc gcagaaacaa ttgctgcttt
    26701 tcttaagttt catgacaaag tagaaattga gcctgaaaaa attattttta ctttctctag
    26761 atctttgcca actgatttaa gttatttagc tgaagcttta cctaaacttg caccatacgt
    26821 atccaaacga actattcaga gccaaattcc atttgtaaca gatgttgatt atgaaaatga
    26881 aatgatggaa ttggaaagtg gaagtgctta tccagatagt gaatacaatt ttggcggagg
    26941 tggcaatagt gacgatagaa acgaagtatt ggaccaaacg tcgagaactg gaggatcaag
    27001 caaggctcaa acaagaaaat cagacactta aaaaattaac tagtgtattt cctgaagcac
    27061 tgaaagagat acaagcaaaa ctattatcac aagctgactt acacaatatc acttatccag
    27121 aaatgatgga gttttacagt acaagtaatc agaaaaaata tcgtgaatat gtggaaaaaa
    27181 attataagtc attaaaaatg tatgatgcaa aatacaaaga gtttatcgat gaattttttc
    27241 caccatttga ctatgcaaaa gtcaatcgct tattacaaat acgatcagat gtatttaaaa
    27301 ttcttgcaga atatgcgatg gatgcagatg tgaatcaata tttttctgat cgcttagagg
    27361 aaattcttca aagaacatat tcttctaatg ctaatgtttt tgttcaactt ttaaacgttg
    27421 atataccaaa ttatttacca gaaaatgaaa ttaaacatta tttgaactat ccatggtcag
    27481 gaaagacatt ttcaagaaga ctttggggaa atatttcatc gcttgaacag aaactctcta
    27541 atgctattgt aaaaagtgtt gctagtggag aaggcgttat acacgcatta aacaccatga
    27601 gattagactc agaaatttgt gacatgttta agttagaaga atcaaagtat aacaaagcga
    27661 tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca agatggtatt gaaaaatcat
    27721 atttagaaac aggtattgag gaatacaacg tattgactgc aaaagatgag agagtttgcc
    27781 ggatttgtgg aggaaaggca agtaagaatc cctataaact gaaagatgct gtcataggtg
    27841 aaaatcgagc acctttccat agtcgttgta gatgtacgga tgttcctaat ttaccaaaat
    27901 taggaaagga tattgatgaa gaatatgacc gtttatttgg cgatttatta gatgagtttg
    27961 cacatgattc ttttggaatt aatttgaaac ggaggaagta gaatgaaaga tttttttgaa
    28021 gcagtactaa caattaatgt aaatgctgat attgcagaag cctacaaaac agctattgag
    28081 tctgagaacc atcctaatgg cttgagagac cattggaatg gcaattatgc ctacgtggtt
    28141 attggcgatc aaactgttaa ttatcaagat aatactccag ttgataagaa taccgttaat
    28201 ttaacgattc aattattatc tcattcatta ccaaatttaa aagaaacagt tgattggtat
    28261 gaaaagatgg gatgtattgt tgttagaact gactacaaag aaggaaagtc tagtaattag
    28321 gcttttttat tttgtccgaa atgacgttaa actagcgcaa tactgggctt ggttgaatgg
    28381 tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc gtggggcgaa
    28441 aggagaatga gcatgaaaca caaatcatta atgccaatga atttgcaata ctttgccgaa
    28501 ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc caatgaagaa
    28561 gctcaaaaat ttattcagtc acaatctcaa agtgttgccg acaaacaatt ggaagcttgg
    28621 aaacaaaata acttagataa aattaaacag gataccatca aggaatatga ggaatctaag
    28681 aaaaacaagt cacctgagca aattcaactg gaaaaactac aagctgaatt tgaagcagaa
    28741 aaagcgttgc gtgtgacaag tgataataaa gcatttgttg cagaacaaat tgctggatta
    28801 gaactagatg gagagttaaa agagtctatt tctcaattta tgctaaataa tcttgttagt
    28861 tcggatacag atttcactaa gaatgctgtt gaaggtttta caagtgtttt gaatgcaatt
    28921 aatgagaaac atgcagatgc attaaaagaa ctacaaatga agtctgcatt tggtggaact
    28981 caacaatcga ataaccaggt tcagcagaac aatgaaacat taacaaatcc agaagaacaa
    29041 ttaggacaaa ttcttcaaca atttaactag gagagtgaaa aattatgaaa aaaacatctt
    29101 taaataattt agagtatttg gatatttcac cagcgattaa tgctatgcaa gtaccaaata
    29161 cacctttttt aagctattta cttggtgctg gaaaaacaga gccagcaaac tcgacagaaa
    29221 ttaaatggcg tgaatatgat atcaacaacg atgattcttc tgaaaaactt gagggcggag
    29281 aatatccaga tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa atttttagaa
    29341 aatcaacctc tgtatctggt acattagatg ctattaatgt gaatggagtc ggaaatgaat
    29401 taactaatca agtagcacta cgtggtatgg aaatgaaaat tgatttgaac cgaaaattga
    29461 ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat gggattttga
    29521 acttgatcaa ttcagcaaat aaggcagaaa cagccactgc gggtgcagta acaagaaaag
    29581 atatcgatac tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa aaactatgct
    29641 tgatttctcc agatatgcag gagttaatga ctgatgagtt agatggaaaa tcaacaaaaa
    29701 ttgttcagtt cggggaaaga gtaacttttg gattgcaaat tggaaatatc gtgtctaatt
    29761 acggtacagg tattgctcta ctagaaccat cattgccaaa aggaacaatt gccgcaatcg
    29821 atactaatta tgtgaaacta cgtccattac gtgaatggag agcagaagaa cttgcaaaaa
    29881 caacggattc tagacgtatc ggtcttgtag gtgaatactc tcttgaatac aatgcttcaa
    29941 actctggggc aattttaaat ttaaagtctg aataaaaggg agttagtact cccttttttg
    30001 ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt cgtgaaatat
    30061 agagtaggta aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg tagatttatc
    30121 acagcagatt caaataacga atttatcatt tctatagatg ataaagaagc aattgcaatt
    30181 ttggaagatg caattgatgt taatgaaatt taggaagtga tctgatggat gaatcgctaa
    30241 aaacggaaat cattgagtct acaaaagaag attttccaga tttgagtgaa gaacgcataa
    30301 ctaatttatt agaaataatt ttgctagaaa ttgaatcata caacacttgt aaaaatgata
    30361 tttcatggga aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata ataaaaaatg
    30421 aatcagaaaa aacagtatct tcagttagac gtggcgatac aacgattagt tatgcttcaa
    30481 caacaaatga tgttagtgag ttacttctag gctatggtga tttgatacga agagttattg
    30541 gttgtggagg attggagttt ttttaatgaa tgaagcagat attttagaaa ctacttatga
    30601 agatagttgt attattgaaa gactgacgga cattgaagat tctaatacaa atattactat
    30661 tcaagattac aaaaaagtat atgataatcc tatttcttgc gctctttcac aaggtcaaat
    30721 cgatggacta gcagtcatag aagatggaga gatggtaaat gtttcaactg acacatataa
    30781 attatttgtt catcctaaga ttaaactaaa aaaaggagat cgaataacaa taactcaaaa
    30841 ggccagtggc ttaatttttt ctctatttgc tactaagcct ttttactatc ctagtcattg
    30901 tgaagtgaat ttgataggaa gtgagaaaaa tgggtaatct caaatatgaa tcaaatgcag
    30961 aaaagatcat tgagaatttt aaaaatatga ctgtaattgc tcaaaaagaa ggaatatcct
    31021 tcgttaacga ttcaatgaat aaagtcgtta gtttagctaa accattaact cctgtaaaat
    31081 caggtaattt aaggcgagga tatcgtgtag taaaagctag aaaactatca agcggtcgaa
    31141 ttgttggaga agttataaac aatgaacatt attttaaata tgtagaagaa ggtcatagga
    31201 ctaaaaatgg tggctttgtc aaagggaaat tcatgttaac tcgtgcaacg aatcttgcaa
    31261 atatgtctta tattcctcga agatttaaac aaatggcaat aaaaatcgtt aagaaaggaa
    31321 agtaacatga aagatgaaat cattgctgca atcagtagca aattaaaaga aatctatcct
    31381 gatgggacaa tatatcttga ttcagttatg cagtcaacta aagattttta tttcgtttta
    31441 tcagtaatgg aatctggaac tgaaaatgta ggaattgata ttcaaaatgt ttctttctta
    31501 attgatattg cattgattga taataaccct aatagaaatt taattaatga attagtctca
    31561 cgttgtggga ctttttttaa tacgattaca atcgatgagc aaatattatt tccaaaagcc
    31621 tatttacctg atgaagcaga tggtgttcaa catattcgtt ttacattaga atttccacaa
    31681 tatattgaat ggagtgaaag ataaatggga gaaaaaagaa gtaaaaccgg aattatttct
    31741 gtcgaaaaac ctacttggtt tcctttggaa gatgagacag ggaattttcc ggtatatggt
    31801 actgcgttgc caatgggaac agcagtaagt atcaaaccaa cagctaatta tgaaacaaca
    31861 caagattatg gggattcagt ggttcaagat caatttacag cttttggtgg tgcagaagtt
    31921 gaactggaag caaatggata tactcataaa gttttatcta caattactgg agggaaaatt
    31981 gttaaaggtg gtgccttgcg ttctggagaa gatattgcgc aagatggtgc gtttgcatat
    32041 agacgtaaaa aatcgaatgg gaaataccgt tatacggtct tttataaagg acagtttgct
    32101 ctagattcag atgaaacatc aacgattgaa ggaagtaaag taagttttac tcatccaact
    32161 tggaagggtt catttgtaga tgtaccagga cttggatata tgtattcagt tgatgaagat
    32221 gatgaaggcg tggataaagc aatgatcgga aattggttta caaaagtagc aattccaatt
    32281 gaagaagcag aactttcagg aggtacagaa taatgtcaaa atatcaaaca acgattaaat
    32341 taacgaaaaa agacgaagaa ggtaaatatg agcaagtaca atttaaatct gctgaatttt
    32401 taccaggaac agttgtagaa gatgctgcag gagttatgga agaaatgcaa acagcgactg
    32461 ataagcaatc agttaaaaaa gctttaagtc gtgcttattc atttattgca gatactttat
    32521 ttgagggaca atttactggt gaagattatt gcaaaggaat tgatgctcgt gagattgcct
    32581 cattgacagg aaaattattg aagtctgtta ctgcaggttt tgatgaaact tatacagaga
    32641 cgaaaaaaaa gtaagtgagg cgctcaagtc accttcattt aagtattcga ttacttaccg
    32701 agaattagat ataaagacgc aattactcga agcaggttgg acgttaccag aaattgaacg
    32761 tactgacttg gatgagttaa tgcgtctttt tgcttttaga gatgcggtaa aagagcatga
    32821 agatgttgag tactatgata atttcactca attttaggag gtgatacttt gaataacgat
    32881 gacctaattc tgaagatgat actagatgaa tctggtttta ctgccggtat gaataatgct
    32941 gtcaaaaagt tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa tagtggcagt
    33001 tcattaggta gtatttggaa aatatttgct ggaagttttc tagctagcgg agtaactaga
    33061 atcgtaggag ctggctttga tctaatcaag ggttccataa gtggagcggt tgatcgagta
    33121 gatacgatga ataacgctct acgaaatttc caaaatatgg gatttagtaa ttcagaaatt
    33181 atgaagaata tcggaaagaa tgggctttta tctcaaggta ttcaaggact tcctactgct
    33241 ttgaatgatg cgataagtca tgttcaactt cttgcttctt ctacaggtga tttaactcgt
    33301 tcaactcaaa tatttaaagc tttaaatgat ggaattcttg gtttcggtgg ttcaactgat
    33361 caagttaatg aagctgttat tcaattatct caaagtttct caaacggaaa agtagacgca
    33421 caaacttgga actcaatgat caacgctcaa cttggaccta ctctttctgc tattgctaaa
    33481 aagatgggaa ttacaatggg agatctgaaa gaaggtttat ctcaaggtaa gatttctgtt
    33541 gaagaattcc aaaatcaatt aatagaaatg gataccaaag gtggcggagg acttaaatca
    33601 ttaagtcaaa tcgctaaaga ttctactaag ggaattaaaa cctctataca aaatgcaaaa
    33661 acagctgtta cacgtggtgt tggtgaggta atagaaggat taaataaagc attagtagat
    33721 tcagatttag gtggatttaa gggaattatt gataaagtgg gcagttcaat ggaatcgttc
    33781 ttaaaagtaa ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa tttatttaac
    33841 gaaattcaaa aatttggttc tgctttgaaa ttcatgatgc cctttcttgt tcctgcagct
    33901 accgcttttg gagcgttcat gtttcaactt aaaggtatac cagcaataat aaaaagcttc
    33961 aataatttta agaatgccat aattggcgtt ggaaattcat taaagattat gggggcaata
    34021 gctgctgcta atccatttgt tttaattatt ggtgcagtcg ttggtgcaat agctgttttc
    34081 ggttacttta tggcaaccaa tgaagagctt agaaacaagg ttatatctat ttggaatgat
    34141 gtgaaagatt ccgtaattgg tgtattaaaa aatataaagg actggggaat tgatacttgg
    34201 aattctgcta aagaaatggc atcaaatgca gttgagggtg tcaaagatgc ttggtcagga
    34261 acaaaggaat ggttttctaa cacctggaaa ggtattaagg atggggccac aggtttattt
    34321 gataaaacag tagagacttc aagaaatgca gtcgatagtg taaaaaatgc atggtctaat
    34381 atgaagaaat ggttttctga tacttggcaa agtataaaag attcagccag agaaaaatgg
    34441 aatggaatca aaggttctat tatgtcagtt gctggtccat tgattacagg tataaagaat
    34501 gcatttttac atgttacttt ttatctagag accttatgga ataatttagt tgagattggt
    34561 aaaaacgtct ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt tattacctcc
    34621 atgatttctg gtggatggga agaaacaaaa aataatatga ttgctgtttg gaataatatt
    34681 aaagaaagtg ccataaatat ttgggaatct ataaagaata tatttgtaag ttattttaca
    34741 aat
//
LOCUS       DAZUKT010000023        34393 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000023 DAZUKT010000000
VERSION     DAZUKT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.023"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1936)
                     /locus_tag="ACG816_001110"
     CDS             complement(44..1936)
                     /locus_tag="ACG816_001110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920001.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1933..3660)
                     /locus_tag="ACG816_001111"
     CDS             complement(1933..3660)
                     /locus_tag="ACG816_001111"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920002.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3676..4326)
                     /locus_tag="ACG816_001112"
     CDS             complement(3676..4326)
                     /locus_tag="ACG816_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4920003.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4530..4826
                     /locus_tag="ACG816_001113"
     CDS             4530..4826
                     /locus_tag="ACG816_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4920004.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5018..5638
                     /locus_tag="ACG816_001114"
     CDS             5018..5638
                     /locus_tag="ACG816_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4920005.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5730..6896)
                     /locus_tag="ACG816_001115"
     CDS             complement(5730..6896)
                     /locus_tag="ACG816_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4920006.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6908..7261)
                     /locus_tag="ACG816_001116"
     CDS             complement(6908..7261)
                     /locus_tag="ACG816_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4920007.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7389..8411
                     /locus_tag="ACG816_001117"
     CDS             7389..8411
                     /locus_tag="ACG816_001117"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4920008.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8430..8591
                     /locus_tag="ACG816_001118"
     CDS             8430..8591
                     /locus_tag="ACG816_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920009.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8850..9284)
                     /locus_tag="ACG816_001119"
     CDS             complement(8850..9284)
                     /locus_tag="ACG816_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920010.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9394..11253)
                     /locus_tag="ACG816_001120"
     CDS             complement(9394..11253)
                     /locus_tag="ACG816_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4920011.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11254..11394
                     /locus_tag="ACG816_001121"
     CDS             11254..11394
                     /locus_tag="ACG816_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4920012.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11371..12531)
                     /locus_tag="ACG816_001122"
     CDS             complement(11371..12531)
                     /locus_tag="ACG816_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4920013.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="ACG816_001123"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="ACG816_001123"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4920014.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="ACG816_001124"
     CDS             complement(14278..14976)
                     /locus_tag="ACG816_001124"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4920015.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="ACG816_001125"
     CDS             complement(14933..16594)
                     /locus_tag="ACG816_001125"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4920016.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="ACG816_001126"
     CDS             16812..18212
                     /locus_tag="ACG816_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4920017.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18464..19543)
                     /locus_tag="ACG816_001127"
     CDS             complement(18464..19543)
                     /locus_tag="ACG816_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4920018.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19629..20588)
                     /locus_tag="ACG816_001128"
     CDS             complement(19629..20588)
                     /locus_tag="ACG816_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4920019.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20600..22111)
                     /locus_tag="ACG816_001129"
     CDS             complement(20600..22111)
                     /locus_tag="ACG816_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4920020.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22133..22697)
                     /locus_tag="ACG816_001130"
                     /pseudo
     CDS             complement(22133..22697)
                     /locus_tag="ACG816_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(22703..23566)
                     /locus_tag="ACG816_001131"
     CDS             complement(22703..23566)
                     /locus_tag="ACG816_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4920021.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23580..24476)
                     /locus_tag="ACG816_001132"
     CDS             complement(23580..24476)
                     /locus_tag="ACG816_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4920022.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24651..25940)
                     /locus_tag="ACG816_001133"
     CDS             complement(24651..25940)
                     /locus_tag="ACG816_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4920023.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25912..27429)
                     /locus_tag="ACG816_001134"
     CDS             complement(25912..27429)
                     /locus_tag="ACG816_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4920024.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27627..28517
                     /locus_tag="ACG816_001135"
     CDS             27627..28517
                     /locus_tag="ACG816_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4920025.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="ACG816_001136"
     CDS             complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="ACG816_001136"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4920026.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29426..30898)
                     /locus_tag="ACG816_001137"
     CDS             complement(29426..30898)
                     /locus_tag="ACG816_001137"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4920027.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31535..31897)
                     /locus_tag="ACG816_001138"
     CDS             complement(31535..31897)
                     /locus_tag="ACG816_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4920028.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32120..32872
                     /locus_tag="ACG816_001139"
     CDS             32120..32872
                     /locus_tag="ACG816_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4920029.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33012..33935)
                     /locus_tag="ACG816_001140"
     CDS             complement(33012..33935)
                     /locus_tag="ACG816_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4920030.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33950..34222)
                     /locus_tag="ACG816_001141"
     CDS             complement(33950..34222)
                     /locus_tag="ACG816_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920031.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10149 a         7165 c         5910 g        11169 t
ORIGIN      
        1 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
       61 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      121 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      181 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      241 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      301 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      361 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      421 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      481 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      541 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      601 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      661 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      721 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
      781 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
      841 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
      901 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
      961 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1021 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1081 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1141 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1201 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1261 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1321 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1381 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1441 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1501 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1561 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1621 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1681 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1741 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     1801 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     1861 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     1921 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     1981 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2041 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2101 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2161 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2221 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2281 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2341 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2401 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2461 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2521 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2581 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2641 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2701 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     2761 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     2821 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     2881 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     2941 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3001 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3061 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3121 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3181 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3241 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3301 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3361 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3421 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3481 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3541 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3601 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3661 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3721 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     3781 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     3841 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     3901 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     3961 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4021 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4081 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4141 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4201 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4261 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4321 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
     4381 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4441 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4501 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4561 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4621 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4681 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4741 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     4801 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     4861 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     4921 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
     4981 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5041 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5101 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5161 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5221 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5281 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5341 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5401 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5461 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5521 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5581 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5641 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
     5701 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5761 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     5821 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     5881 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     5941 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6001 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6061 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6121 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6181 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6241 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6301 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6361 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6421 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6481 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6541 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6601 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6661 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6721 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6781 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     6841 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     6901 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     6961 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7021 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7081 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7141 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7201 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7261 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7321 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7381 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7441 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7501 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7561 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7621 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7681 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7741 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7801 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     7861 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     7921 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     7981 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8041 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8101 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8161 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8221 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8281 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8341 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8401 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8461 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8521 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8581 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8641 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8701 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8761 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     8821 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     8881 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     8941 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9001 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9061 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9121 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9181 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9241 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9301 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9361 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9421 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9481 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9541 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9601 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9661 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9721 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9781 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
     9841 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
     9901 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
     9961 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10021 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10081 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10141 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10201 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10261 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10321 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10381 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10441 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10501 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10561 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10621 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10681 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10741 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10801 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    10861 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    10921 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    10981 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11041 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11101 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11161 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11221 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11281 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11341 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11401 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11461 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11521 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11581 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11641 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11701 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttacctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt
    18301 ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga
    18361 gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga
    18421 cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt
    18481 tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt
    18541 tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg
    18601 tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga
    18661 tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag
    18721 ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta
    18781 ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca
    18841 aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt
    18901 gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt
    18961 ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt
    19021 cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg
    19081 ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat
    19141 aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta
    19201 aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa
    19261 aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg
    19321 ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat
    19381 agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata
    19441 tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga
    19501 tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt
    19561 atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt
    19621 tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc
    19681 cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg
    19741 ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta
    19801 aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc
    19861 gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg
    19921 cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg
    19981 cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca
    20041 ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga
    20101 taaagattag aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt
    20161 ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt
    20221 cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg
    20281 cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac
    20341 cattttccat tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct
    20401 tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc
    20461 cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct
    20521 gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc
    20581 ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa gagtattttt
    20641 ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta
    20701 ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt
    20761 gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg
    20821 tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat
    20881 aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata
    20941 cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg
    21001 cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac
    21061 taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg
    21121 tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc
    21181 ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac
    21241 ttctcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga
    21301 atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag
    21361 tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct
    21421 cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag
    21481 agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt
    21541 catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta
    21601 gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc
    21661 ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt
    21721 catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga
    21781 tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat
    21841 caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg
    21901 taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga
    21961 catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat
    22021 agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta
    22081 ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt
    22141 catttcctat agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa
    22201 taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa
    22261 tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg
    22321 tgatatcttt gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa
    22381 gtaaaactac atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa
    22441 caagaaaata aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct
    22501 ttattccttg aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca
    22561 ttacataaaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
    22621 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta
    22681 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
    22741 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
    22801 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
    22861 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
    22921 tcatcagtgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
    22981 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
    23041 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
    23101 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
    23161 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
    23221 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
    23281 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
    23341 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
    23401 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
    23461 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
    23521 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
    23581 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
    23641 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
    23701 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
    23761 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
    23821 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
    23881 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
    23941 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
    24001 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
    24061 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
    24121 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
    24181 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
    24241 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
    24301 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
    24361 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
    24421 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
    24481 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
    24541 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
    24601 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
    24661 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
    24721 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
    24781 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
    24841 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
    24901 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
    24961 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
    25021 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
    25081 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
    25141 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
    25201 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
    25261 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
    25321 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
    25381 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
    25441 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
    25501 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
    25561 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
    25621 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
    25681 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
    25741 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
    25801 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
    25861 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
    25921 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
    25981 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
    26041 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
    26101 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
    26161 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
    26221 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
    26281 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
    26341 ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc
    26401 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
    26461 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
    26521 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
    26581 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
    26641 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
    26701 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
    26761 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
    26821 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
    26881 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
    26941 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
    27001 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
    27061 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
    27121 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
    27181 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
    27241 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
    27301 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
    27361 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
    27421 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
    27481 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt
    27541 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
    27601 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
    27661 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
    27721 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
    27781 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
    27841 tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt
    27901 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
    27961 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
    28021 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
    28081 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
    28141 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
    28201 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
    28261 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
    28321 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
    28381 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
    28441 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
    28501 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
    28561 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
    28621 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
    28681 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
    28741 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
    28801 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
    28861 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
    28921 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
    28981 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
    29041 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
    29101 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
    29161 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
    29221 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
    29281 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
    29341 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
    29401 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
    29461 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
    29521 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
    29581 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
    29641 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
    29701 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
    29761 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
    29821 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
    29881 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
    29941 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
    30001 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
    30061 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
    30121 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
    30181 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
    30241 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
    30301 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
    30361 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
    30421 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
    30481 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
    30541 gcccttccac ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac
    30601 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
    30661 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
    30721 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
    30781 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
    30841 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
    30901 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
    30961 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
    31021 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
    31081 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
    31141 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
    31201 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
    31261 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
    31321 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
    31381 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
    31441 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
    31501 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
    31561 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
    31621 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
    31681 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
    31741 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
    31801 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
    31861 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
    31921 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
    31981 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
    32041 cgtgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
    32101 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
    32161 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
    32221 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
    32281 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
    32341 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
    32401 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
    32461 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    32521 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    32581 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    32641 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    32701 ccaagctcat aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa
    32761 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    32821 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    32881 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    32941 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    33001 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    33061 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    33121 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    33181 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    33241 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    33301 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    33361 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    33421 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    33481 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    33541 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    33601 gcaaattctt caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc
    33661 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    33721 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    33781 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    33841 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    33901 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    33961 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    34021 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    34081 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    34141 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    34201 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    34261 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    34321 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
    34381 attattagtg aat
//
LOCUS       DAZUKT010000024        34392 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000024 DAZUKT010000000
VERSION     DAZUKT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.024"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..2096)
                     /locus_tag="ACG816_001142"
     CDS             complement(12..2096)
                     /locus_tag="ACG816_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4920032.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2238..2360)
                     /locus_tag="ACG816_001143"
     CDS             complement(2238..2360)
                     /locus_tag="ACG816_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920033.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(2621..2980)
                     /locus_tag="ACG816_001144"
     CDS             complement(2621..2980)
                     /locus_tag="ACG816_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4920034.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3010..3351)
                     /locus_tag="ACG816_001145"
     CDS             complement(3010..3351)
                     /locus_tag="ACG816_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920035.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3348..4022)
                     /locus_tag="ACG816_001146"
     CDS             complement(3348..4022)
                     /locus_tag="ACG816_001146"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4920036.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5198..6496)
                     /gene="asnS"
                     /locus_tag="ACG816_001147"
     CDS             complement(5198..6496)
                     /gene="asnS"
                     /locus_tag="ACG816_001147"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4920037.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6536..7726)
                     /locus_tag="ACG816_001148"
     CDS             complement(6536..7726)
                     /locus_tag="ACG816_001148"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4920038.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7747..8274)
                     /locus_tag="ACG816_001149"
     CDS             complement(7747..8274)
                     /locus_tag="ACG816_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4920039.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8321..11110)
                     /locus_tag="ACG816_001150"
     CDS             complement(8321..11110)
                     /locus_tag="ACG816_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4920040.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11257..11457)
                     /locus_tag="ACG816_001151"
     CDS             complement(11257..11457)
                     /locus_tag="ACG816_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4920041.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11910..12230)
                     /locus_tag="ACG816_001152"
     CDS             complement(11910..12230)
                     /locus_tag="ACG816_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920042.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12733..13851)
                     /locus_tag="ACG816_001153"
     CDS             complement(12733..13851)
                     /locus_tag="ACG816_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920043.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14084..14206
                     /locus_tag="ACG816_001154"
     CDS             14084..14206
                     /locus_tag="ACG816_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920044.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            14322..15629
                     /locus_tag="ACG816_001155"
     CDS             14322..15629
                     /locus_tag="ACG816_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4920045.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15750..16949
                     /locus_tag="ACG816_001156"
     CDS             15750..16949
                     /locus_tag="ACG816_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4920046.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(16976..18244)
                     /locus_tag="ACG816_001157"
     CDS             complement(16976..18244)
                     /locus_tag="ACG816_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4920047.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(18300..18366)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(18414..19055)
                     /locus_tag="ACG816_001158"
     CDS             complement(18414..19055)
                     /locus_tag="ACG816_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4920048.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(19464..19967)
                     /locus_tag="ACG816_001159"
     CDS             complement(19464..19967)
                     /locus_tag="ACG816_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4920049.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(20090..20854)
                     /locus_tag="ACG816_001160"
     CDS             complement(20090..20854)
                     /locus_tag="ACG816_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4920050.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            20962..21237
                     /locus_tag="ACG816_001161"
     CDS             20962..21237
                     /locus_tag="ACG816_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4920051.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            21797..22747
                     /gene="yidC"
                     /locus_tag="ACG816_001162"
     CDS             21797..22747
                     /gene="yidC"
                     /locus_tag="ACG816_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4920052.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(22793..23194)
                     /locus_tag="ACG816_001163"
     CDS             complement(22793..23194)
                     /locus_tag="ACG816_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4920053.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(23208..24017)
                     /locus_tag="ACG816_001164"
     CDS             complement(23208..24017)
                     /locus_tag="ACG816_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4920054.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(24004..24894)
                     /locus_tag="ACG816_001165"
     CDS             complement(24004..24894)
                     /locus_tag="ACG816_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4920055.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(24907..25386)
                     /locus_tag="ACG816_001166"
                     /pseudo
     CDS             complement(24907..25386)
                     /locus_tag="ACG816_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(25562..26503)
                     /locus_tag="ACG816_001167"
     CDS             complement(25562..26503)
                     /locus_tag="ACG816_001167"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFS4920056.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(26500..27495)
                     /gene="lacD"
                     /locus_tag="ACG816_001168"
     CDS             complement(26500..27495)
                     /gene="lacD"
                     /locus_tag="ACG816_001168"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4920057.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(27488..28684)
                     /locus_tag="ACG816_001169"
     CDS             complement(27488..28684)
                     /locus_tag="ACG816_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4920058.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(28702..29430)
                     /locus_tag="ACG816_001170"
     CDS             complement(28702..29430)
                     /locus_tag="ACG816_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4920059.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(29871..30845)
                     /gene="lacD"
                     /locus_tag="ACG816_001171"
     CDS             complement(29871..30845)
                     /gene="lacD"
                     /locus_tag="ACG816_001171"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4920060.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(30842..31300)
                     /locus_tag="ACG816_001172"
     CDS             complement(30842..31300)
                     /locus_tag="ACG816_001172"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4920061.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(31297..32760)
                     /locus_tag="ACG816_001173"
     CDS             complement(31297..32760)
                     /locus_tag="ACG816_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4920062.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(32742..33674)
                     /gene="pfkB"
                     /locus_tag="ACG816_001174"
     CDS             complement(32742..33674)
                     /gene="pfkB"
                     /locus_tag="ACG816_001174"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4920063.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            33816..>34392
                     /locus_tag="ACG816_001175"
     CDS             33816..>34392
                     /locus_tag="ACG816_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4920064.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT        10016 a         7428 c         5864 g        11084 t
ORIGIN      
        1 aaagaggacg ttcattcgtc ctcttttttt acatgcaagc ttcgcatggc attcaaaaca
       61 gctaatactg tcaccccaac atctgcaaat actgcatctc ccattgatgc aaaaccaaaa
      121 gcacctaagg caagtaccaa aatttttatc ccaatagcaa agacaatgtt ttgtttgacg
      181 attttcaatg ttttctttgc taaatggatt gcttctgcaa tcttcgatgg ttgatcattc
      241 atgatgacta catctgctgc ttcaattgca gcatcgcttc ccaatccacc cattgcgatt
      301 cctacatctg ctcttgctag caccggtgca tcgttgattc catctccgac aaatgcggtc
      361 tttttattgt ttctttgtaa gatttcctct aaccgctgca ctttatcttc aggaagcaat
      421 tcgctatata tcttgtccac gcctacttgc ttcccgatat gatcagcaat ttttttcgag
      481 tcgcccgtca acatgactgt gttcttcaca ccagcctgtt ttaattgaac caatgcgtct
      541 acggcatctt ctttcaaggt atctgcaata acaagataac cactgtaact ttgatcgatc
      601 gctagataaa gcaatgtacc gatttcttga cttaaaggaa aagagatttc aaatctttcc
      661 atcaatttgt gattacctgc taatacttgt ttgccatcaa cttcagctaa gatcccttca
      721 cctgcaaatt cctggatatt agttgttggt aaaagcggac ctgcgtagcc ttctaaaaca
      781 gattgagcaa tcggatgtgt agaaaactgt tctgcactcg caatatattg catgaatttc
      841 tttggatcga tttttgtatc gatcgtctgg atcgaaaagt cacctttcgt taatgtgccg
      901 gttttatcga ataccactgt ctcggtttgc gcaagcaact ccaagtaatt tgaacctttt
      961 actaatacac cgattttact tgcgcctccg ataccgccaa agaaactaag tggcacggaa
     1021 atcactaatg cacatggaca tgagatcact aaaaatgtca atgctcgata gatccattca
     1081 gaaaacgccg ctccagaaac aacaagtgga ggtaaaacag ctaataaaac ggccaatcct
     1141 acaacgactg gtgtgtaata gcgcgcgaat ttagtaatga aattttcagc tggtgctttt
     1201 ttactgctgg cattttctac taattctaat aatttgctga tagtagaatt ttcataagat
     1261 ttttctactt gaactaaaaa tggtttattt ttattgataa atccgcttaa tactgtttca
     1321 ccaacatata cagatttagg gactgattct cctgtaagtg ctgacgtatc gactaatgat
     1381 tctccttcga tgatcgttcc atctaacggt acacgttcac caggcttcac aagtacaaac
     1441 tgaccgatct gcacgtcttc agggttcacg gtcatcaatc cttccgtcgt ctgcacgtga
     1501 gctgtttctg gacggatcgc catcaattcg cgaatcgatt ttcttgaatg actgactgcc
     1561 aatccttgga aatactcgcc gatttgataa aatagcatca ctgctacggc ttctggatat
     1621 tcaccgatca acatagcgcc aatcgtagca acagacataa gaaaattctc atcaaagatt
     1681 tttcctttga cgatattcat cactgccttc ttcactacat catatcctat cagcagataa
     1741 atcaataaat accctatcaa gcgaattggt tcattaaaat caaataatag caataaagcg
     1801 atacctgcaa gggaaaataa aatccggaac agcgtatttc tctctttttc atttccatgt
     1861 tcagatactt tttccttatc tatttctgtt actttgacat ctggttctaa ttccttcact
     1921 actttttttg tttcttgttc aatcgtagcc aaatgatctt cctctgcgtc aatcgttagt
     1981 tttccagaag tgaagtttac cgttgcttcc ttgactccat tgatcttctg aactcctttt
     2041 tcgattttca ttgcacagtt cgcacaatct aaaccttcta atctgtaact tttcatttta
     2101 atgacctcct cgcctcaaca tatgaacatc tcttcataac taattataaa tgaatggtta
     2161 ttcatatgtc aaggtatccg aaagaaaaaa tgaaaaacat ctgcatcgga aaatttcttc
     2221 catacagatg ttttggctta ttctttcttt ttctcttcga tcattgcccc tgctcttatc
     2281 caacccgcac ttgccttctt gatcttgaca ggatagcaac taagtttgaa gcctgttgct
     2341 ggcaatttgt ctaaattcat taatttttcg ataggacaat atcttttttc aatccctgca
     2401 aaatgtcctt cccagatatt cgaagctcca ctacgctttc aaaaatatag aaagagttct
     2461 ttaatgaaaa ttatttcaac tttccataaa cagtaatccc atataaataa aagacgtaaa
     2521 agtcacatgg gagaacttat acaggaaaac acatagagtc aggtgaaata catgtatccc
     2581 acctgactct atgcgttttc taacctttat tattctcttt ttatacaatt tctgcaccga
     2641 atggcatcaa cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga
     2701 tcgtgataca tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa
     2761 cgatcagcca gatgatatta acgatcaatg acatgccaga ctggtgataa attacttctt
     2821 tgccaaaagg cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac
     2881 ctatgatcgt gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc
     2941 cgccgaatat aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac
     3001 tgtttttgat taaaactggg caaaaattga ttttattctt ctttttttag cttccagcat
     3061 caagtattct gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg
     3121 ttggattagt tttcctgatg gtcgctgatc cacccgttct tctgactgtt gcacgtccgc
     3181 ttgcgcaaat ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt
     3241 atcatttagg tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag
     3301 ctgattgatt tgaggtaaat cttcttttac tgcccaccgt atttccatca tttctcactt
     3361 ttctttcgct catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag
     3421 ctttgtgcaa tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat
     3481 ttcgaaaaac cataagcaat ccctaaagca ttgaatatgc catctaaagg aaccagcaat
     3541 gcgccatatt ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc
     3601 cagccatgac ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag
     3661 atttccgctg tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgcgca
     3721 gatttctcag ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg
     3781 atcaaaatcg atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc
     3841 agatctgcaa ttttgtctcc tcctatccca cggttcatgc attcaaattt catcgttgga
     3901 tattgggaga ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct
     3961 tctgctcggt ctcttcctgc gtctgtaatg ctgtcaccga taaataatat acgttttttc
     4021 atttgtcaaa aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa
     4081 gccctatcaa gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc
     4141 aatcaattga atgaacgctc atcaagtgtc tagtttttta tacttttttt cttaacgtgc
     4201 cggattcccc ttgattcctt cgttgtttgg tccagagaaa tcgatcacac ctaagcttcc
     4261 gtcaatcaga ccactgatga cgccatcaga acatatattt ataggctcat ctaagacttc
     4321 cagtaaatgt tcttttacta atatctcttc tagattgctt gggatagatt ggtcataact
     4381 ttatctggtc cgatgatttc aaggatgaat taaacttaca tatggatcat tgcatgctga
     4441 aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg tactagatgc
     4501 tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat aaccttcttt
     4561 gtatatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct
     4621 tttgctttct tctcttgtct gctggaaact tcctttatcc cgtattttaa aaaaagaact
     4681 gcgagagttc gcagttcttt tttttaaata aacgttgctc tccggccaac tcctcggtca
     4741 caagtcacaa tagtcatcaa acatgaaaaa ctgttttttg aaattactcc ttgtgactcg
     4801 gagctgaacg gcaggcgcac aacctcttga tataaacggt gttcaatcag ctgaccctcg
     4861 atattagcta aaaaaacggt aaaatatgag aagctatttt tccgttttgt ttctctaata
     4921 ctcaggtcaa ggcactgatt tcacaacctc ttccttaaac ggtgttcaaa agccagtttc
     4981 tcggtattaa gccaaattta tgcaaaaatg tggaaagcca tttccacaaa atttttctta
     5041 atactcgaaa ctaaacagct ttttcacaac ctctccacta aacggtattc aaaagcttac
     5101 atctgcggta ataagtcccg ccaattcaaa aacatgaaca gctgtttttg cttggctgtt
     5161 ctaattactt gatgcagaac agctttttca tcacctctta tggataaatt cggttcaaca
     5221 aacgtgggaa tggaatagct tcacgtacat ggtcgatacc agcaatccaa gtgactgttc
     5281 gttcaaggcc tagaccaaaa ccagagtgcg gaacagaacc gtatttacgt agatcaagat
     5341 accatgaata ttctttttca tccaaaccgt gattacggat ttgttctagc aagaaatcat
     5401 agtctgttgc ccgttcgctt ccgccaataa tttcaccgta accttctggt gcgatcatat
     5461 ctgcacaaat taccacgtct tctcttgttg gatgtggttt catgtagaat ggcttgatgg
     5521 cttttggata gtttaggata aagacaggct gttcgaatga attcgcaatg aacgtttcat
     5581 gaggtgaacc aaagtcttcg ccccattcga tgtcatcaaa tccatttttc ttcagtaatt
     5641 cgattgcatc atcgtaagta atacgaggga atggcaattt tgtataagtt tttaagactt
     5701 ctttgtctcg tcctagtaca tctaatgcat attcgcaatg atctaaaaca ttttgtacta
     5761 agaatgctac atattgttcc tgtacttcta aactttcttc ttgatgagtg aaagccattt
     5821 ctggttcgat catccagaat tcgatcaaat gacgtcgtgt ttttgatttt tcagcacgga
     5881 aagtcggacc gaatgaaaag acttttccga aagccatagc agcagcttcc atgtataatt
     5941 ggccactttg agataaataa gctttttgat caaagtaatt tgtttcgaat agatccgtag
     6001 tgccttctgg tgcagaacca gtcaagatcg gcggatcgat tttgacaaaa tgatgttgat
     6061 taaagaattc atacgttgca cgaatgattt catttctgat ctgcataatc gcatgttgtc
     6121 ttgaagaacg cagccataaa tgacgatgat ccatcaagaa atctgttccg tgttcttttg
     6181 gtgtgatcgg ataatcgtgg ctctcgctta ccacttccac atttgtaatt ccgatttcat
     6241 aaccaaattt tgaacgactg tcttcacgga tttctccagt tactaacacg gatgtttctt
     6301 ggtgtagatt tttagctgtt tggaaaactt cttccgacac ttcgcttttt acaactacac
     6361 cttggaaaaa ggctgttccg tctcttaatt gtaaaaatgc gatttttccg cttgatcgct
     6421 tatttgcgac ccaagcgccg attgttactg tttctcccac atggtttttc gaatcgatga
     6481 tttgaatttg ttccacgtat gactctcctt ttatattcct tgaatttctt caggattaat
     6541 tttgactttt actctcaata aattgcttga ttctgcgtac cgcttcttct aacgtagcgc
     6601 gatcagtggc ataactcatg cggacatttt ctggtgcccc aaagccttcg ccagtcacaa
     6661 gcgctacccc tgtttcttct aaaagatctt ctacccattg tgtgacattt tcatacttac
     6721 acatgcgtag cgtttctttt acattaggaa aaagatagaa agctccttgc ggcttttcaa
     6781 gagaaacacc cggcagttct gccacaagcg gatataacgt attcaggcgt tcttcaaagg
     6841 cgttccgcat ttcttctact gtatcttgct cacctttcaa tgcttcaaca gctgcatatt
     6901 gactgactgc tgtcggattg ctagttgatt gggaagcaac agcgatcatt ccattgatga
     6961 tcacaggatt tcccaccgca taaccaatgc gccagcctgt catcgcatat gttttagaca
     7021 cgccgttgat gatgatggtc tgatttttga ttgcttctga aatcgttgca attggagtaa
     7081 actcattgcc attataaact aaacggccat aaatatcatc agcaactatc aaaatatcat
     7141 tttttactgc ccattcaccg attgcctgca gttcattctt gctataaatc attccagtcg
     7201 gattcgatgg agaattgatg attacggctt ttgttttttc tgaacgtgct tgttctagct
     7261 gttcaacagt aactttccag ctattggatt cttccccttt cacaaagaca ggaagtcctt
     7321 ctgccagttt tacttgctca ccgtaactga cccagtaagg cacaggaatg atgacttcat
     7381 cttgcgggtc aaggatcgtt tggaaaaggg tatacaaagc aaatttcgct ccgtctgtta
     7441 cgactgtttg tgaaggttca tagacaagtc catagttttt cttcagataa tcaacgattg
     7501 cctgtcgaag ctctgggatt cctgctgttg gtgtataata gctagctttc ccattgcgga
     7561 ttgcttcgat tgctgcttct tggatgtttt ctggtgtcgc aaaatccggt tctcccacag
     7621 tcaaactcag cacatctctg ccttttgctt ttaatgcctt ggcctttgcc gcagctgcca
     7681 aagtgacaga cggttctaat cgttttgttc tttttgataa ctccatccca ttcattcctt
     7741 taccttttaa attcccttga ttgtcttgac ttcatctcca gattcaaagc caatcaggta
     7801 ataattcagt ttcccatttg tttcttttcc tactacttcc cacacaggct gatcatcata
     7861 catccctaaa gcagcttttt cgatttggat ttcaggatgg gccgcagcaa ttgtctgttt
     7921 tgcttcgttt tcagtcagtc cctttgtttg atccaatact tttacttttt caccagattt
     7981 aggaatgata acggcgattt cttttccttt atcatttgta cctgtcagtg aaaagtatgt
     8041 cttctttcga gtaaaccaat aaaaatgatc ggtttcctca agatttgcat atttctttgc
     8101 gatttctact gcttctttct tcgcctgcac cgccggacga ctggaacgca agtaaaaaat
     8161 cgagctgatc acgatgaccg ctaataaaaa tacgatcaag ccaagtaaca cgcgattaat
     8221 cgttttttcc tttgtctgtt ttttcttcaa gggtcgtcgc tcctttatct tcattccttt
     8281 tcattatagc agattttaat tatgaaaaaa gacagctcct ctaacttcct tcttcatttt
     8341 tctttaagaa ttcatggata tcctgataga tttcctccat agctagctct ttgatcggta
     8401 cttttttcgg taatgcatgg atgatccgtg cgccatattt tgcagtgact agtcttcgat
     8461 ccaaaataag caatacgcca cgatctgtct ccgtccttat caagcgacct aatgcttggc
     8521 gtaacttcaa agctgcttta ggtacggatt cctggtaaaa aggattgatc ccctctgatt
     8581 ctaaataagc gttcctagct ttgaccatcg ggcgaagcgg attatcgaag ggtaaacgtg
     8641 tgactacaac gatctgcaaa gcatctccag gcaaatctac accttcccaa aagctgtcag
     8701 cgccaaacaa tacgcttttt tctgacaaaa tgaagcgttt caaaagcttc tcccggcttc
     8761 caccaaaccc ttgagctagt atttctcgtc ctttttctag catagacaga tgcattttct
     8821 gatagacctt ctgcaaaaca tcatgagaag tgaataacac aagtattggc cgatcttctt
     8881 gttcgatcag acctgtaagt gctgtctcta aataatcgat atatgcttga ttgctgactt
     8941 gatggacggc aggtgcctct tttggcacat agatccttgc ctgtttctca taatcataag
     9001 gagaggcaat cactttaaga ggtgctgctt caatgccaag cctcttagag agatagtgtc
     9061 gattagggcc gatcttcagc gttcctccaa tataaagaat acgatcatat cgaggatacc
     9121 aaacagtcga cggcaacata gccgcttgaa aatcatggag atgaaaaatc atctgctgcg
     9181 ctgtgttccc aggaacgaac caatggatca aatgatctcg ccagtcttcc agccattgat
     9241 ccatcagcaa gtattgcttg tctaattgat caaataggct atataaactt gctaaatcga
     9301 tccgcatctg gtttgtccag ctttcttgga taatctcaaa agtcagacgc atttgtgact
     9361 gtagctgctg gatctcctga taaaaaagct gcaggtgatc aatggctgtc ccagattcca
     9421 gtggcagctg atccaacatc tcttttgtca tcatgacttc ttctggatac tgtccgttag
     9481 gttgcttgcc gctcactaaa ctcattaatc cttctgccat acaaatctgt gcttcgcata
     9541 gcgcagaaag ttcttctcga taaatcgata accagcgtaa ggcttcatgt tctttcggta
     9601 aatagctttg tattcggtca aacagctgat ttggttcttc taattgctgg atttgtttat
     9661 ggaaatagtt aatacttaat cgtttgtcat ttactttttc agcgatttcc ggcaaatgat
     9721 gtacttcatc gatcaacaaa aatggactag ctggaagcaa tggttctttt cgctgtgttt
     9781 cttgtactaa aaacgcatga ttgacaatca aaaaattact ttgcctgatt ttcttttgca
     9841 agaaacgaac aaagtcttgt tcgtaaaatg actgatttct tgctaaaaat ccaatacctc
     9901 tgtgcgcgat atctgcaaag aatgaatggt tcaaatttgt taaatgtaat tcatccaaat
     9961 caccagtctt cgtttgcgtc agccaaacta agaccgccat ttgatacaaa gcatattgtt
    10021 tttgttctgg cggttccaca agtgtgcctt taaaacgctg caaatcaata tagtggcgat
    10081 agcttttgat aattacaggt tgtatgggct gatctagcaa ttgattcagc aatggaatgt
    10141 ctttttctat gatctgctgc tgcaaaacta gcgaaacggt actgatgaca gcgggttttt
    10201 caggtgtagc caaataactc aatggcagca gataaccgat tgttttaccc attcctgtag
    10261 ctgcttcgat catcaaatct tttgattcat cttttgtgaa atggtcgtag accgcattca
    10321 tcagtctgtt ctgttctttt cgatacatca gtgttttacc aaacattttt tcttttgctt
    10381 gtttttttct tggatatgtc cgctcgccat aatggacgga tgtgaatagt tccacttctt
    10441 ttttctgcaa agctagccca tcgacgattt ctaatgaggg atcaagtggc tctggttttt
    10501 ctttcatttc ttcaatcaca tgataaatga aacgactcgt atccatacct gtgtgtctac
    10561 ttaatcgcgc aatctgctct aacgtgatga ttggcaattt tctcatccgt tcttcgatca
    10621 gcaaaagaag ttgacctgtc acttcggcat cactatctgc ctgatgagga ttctcatgaa
    10681 taaatccaag gctttctgac aaatcagcta agcgaaagct tggttccgtc ggcaagaaaa
    10741 tttgtgcaag ttctactgta tcgatgcctg gtatcttcaa actaggaagt ccgcatcgca
    10801 tgagttcatg gttcaaaaaa ttataatcaa aataaatatt atgcgcgaca aatatggtgt
    10861 ctgccaaaag attgtaaatc gtttgggcga catcttcgaa ataaggcgct ttttggacgc
    10921 gttgattcgt gatccctgtt aaatgctgga tttgttttga aatcttccgc cctggatgaa
    10981 tatcgatcga aaaacgtgaa gtgatccgac ctgattcaat catgacgcag ccaaattgga
    11041 tgatccgatc ttcttttgga ttcgtcccag tcgtttcgat atcaacgact gcataagttc
    11101 ttggtttcaa aatatcttcc tcttctcctg cctctaatgt tcttttgaaa ttatactaca
    11161 aagtgcagaa aaatactctc atcgcttttt aaagaaaata gacaaaaacg cccagacacg
    11221 caaatgtctg ggcgtttatt ttttagtata ggttaattaa gctttagtaa cgtttgttgc
    11281 ttgaggtccg cgttggcctt cttcaacatc gaaagttact gcttggcctt cttctaaagt
    11341 tttgaatccg tcgccttgga tagctgagaa atgaacgaat acatcgtttc cgttttctgc
    11401 agtgatgaat ccaaatcctt tttctgagtt aaaccatttt actgtacctg tttccataat
    11461 aaaaatcctc cttgtgtatc aacacatatt ttgcaattac ttgaatggtg tatatctgga
    11521 agatgtttat ttgaaacaag ctaccacaga ttaccgaaca aaaactactc ctatagtata
    11581 tcataccgtt tacaaaaaag ctagagtttt tcgacttttt ttattaaaaa aagttttctc
    11641 agcgattaca taaagtgaaa aaccgaactt ttttttaaaa taaacgacat attgttcagt
    11701 tggtgttttt ttgctctgat gttctttttt aaactaaaaa aattattctt tctgactact
    11761 tatcggttct gtttcaacgg tcgaggctgg ggaagattct ttagtaggtc tgatgttctc
    11821 atggtatttc tgatagccta tgacagttac cagaagaaca aggcaaacca atacgaccaa
    11881 cgagattagt ttttcttttt tgctcatatc tatacctcat aaataacagg atgatgtcca
    11941 tcatatttct gagtatgagt aaaagcaaaa gctgcagctg tagaatgtaa gaaatgccag
    12001 caactttcat aatcacaggt cggacagtac caaaagtaac caaatcttgg ctgcaaagac
    12061 cgttcttctg tttcaggaat cgcagaatca agatctggtg tctgtacata aacatttttg
    12121 tacagacgtg tgcctgtaat aggaaaccgc tgtgtttctt cagcaaaaac tggctgactt
    12181 gttaaactta gggatagaga cagcaaacac cataacagtc tttttttcat aaagacctcc
    12241 ttatcgataa gtttttccca attaaaatca tatctcaaga acgcccgtta ttcacatttt
    12301 gttcataaat gatgtttttt gtatcataat cgtcaaaaag aggtcgtgac cacgtcacaa
    12361 cctctttttt aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcacaa
    12421 aaatttgaga aataattttt gtcaatctct tcttatttct tgaagctgac cacttctgtc
    12481 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaccaaac
    12541 aaaaaatatg aagagctatt tttgcttgct gttcttatta cttgatgcag aacagctttt
    12601 tcacaacctc tcatacacgg tgtttaatca gctgaccttc ggtattagct caaaaaacgg
    12661 caaaatatgt gaaactattt ttccgtttgt ttctctaata ctcaggtcaa aacactgatt
    12721 tcacaacctc ttttaataac gaagaatcgc tgtcagactc ttttcatctg gcgcatcttt
    12781 ttgatccaaa ataaacactt ttccgccatt aatgatcaca tcatctgcta atgcattcaa
    12841 cacttgacgc cgatcatatt ctgtttcagg atcttcaccg aaaccatcag ctaagtttga
    12901 cgtagcgata aataaatgtg acacttttcc ttctttcgca gctggaacga tatcaactaa
    12961 ctgatcgatg aatttcttat ctaagagttt attgtattct tttgattctt gagctgcgag
    13021 ttcacttgac agcttcttcg ccccttcttc gatatctcta aaggatagct gcgcaggaga
    13081 agcagaaatc gcaacgtctg cacaataata gggattttta gcaatttttt tgaacatcgt
    13141 ctgattttct ggtagagcaa ataaataaat cggccattgt tctggatttt cgaaagaatc
    13201 cttcaagtac gtatcaactg cttggtaata gttgacccaa tcgatctcta cttcttcatc
    13261 ttttggattt acgccatgga aagttactcc atctcgggct ccgcctcctc cattagaaga
    13321 gaagttcaaa ccgccacctg tcagttcaga tcccaatgca gtaacgacat caaccggtgc
    13381 cccttctggt agagatactt cttttacttg tttgttgctg acttcataca atttcatgga
    13441 gtcgcggttc aatcccaata aataataaga atagttgaac tgcccatttt tgataatagc
    13501 cagtaaataa ggaagatcac ttacatagta ttgatcgtct accgggatac tcaaacgatg
    13561 gatataggtt tggtcagcag ttataatgac cgctacactc gtagttgcac tacgccaaaa
    13621 cgaaggatct cctaacaaat tttcaatttt ttcttggaac ggggaccagt catgttcgga
    13681 atattttttc tcaaaacgtt tctttgcttc tttcgcaaaa tttttgaatt tgatctgatc
    13741 tttttccaca tcttgatggg cgacatgcgt attcagcatg atcgtgacga atggtccttt
    13801 agcgccttct gaagtcaatt gtgacaataa ttctttatcc ttttttgcca taaacgtacc
    13861 cctcctaaaa aaatcattcc tttgtttcta tctaaagtct accacaacac tatttttcca
    13921 tacaataaaa cgtatctaaa aaaataaaac ggtttctacg gttttaaatt tagctatttt
    13981 tttaagttat cttcataatt tcctcaatta tttagcccca gtcttcaact attcttcatg
    14041 aaatgctgat aaactaagaa aaagaataaa agaggtggat cttatgcatt tgattatgat
    14101 tttcagttta ttaagtatta tcagtttatt tgctttcagc gcttcccgct tattagatgc
    14161 ggaaacacag cctattcctg taaaagtacg taaaaacgac cgctaacctt ccataacgtg
    14221 cgttcttatc ctaaagtttt ttcagaaaca ttgaaactct ctcccctctc tgataaaatt
    14281 ttaatttgta gcgaaacaaa ttaattacaa ggagatcgga tatgtccata aaaaaaacac
    14341 gtctagtcct attattggct agtctcattc tttgccttgc ttcttttcta ccagctgttg
    14401 ctgtgcaagc tgaagatact ttcaaagtaa atgcaaaagc agcctttgct gtagatgcgc
    14461 aaagcggtaa gattctttat gatcaagatg gagaaaaaac catggggatc gcttcaatca
    14521 caaaaatcat cggtctttac atcgtccttg atcaagtaaa agaagggaaa ctatcatggg
    14581 atgataaagt ttcaatttca gattatgcag aaaatttgag tgtcacccca gatttatcaa
    14641 atgttccttt gcataaagaa aacacctaca ccgttaaaga attattcgac tctgcaatca
    14701 tccagtcagc caacgcttca atggttgcct tggcagaaaa aatctctggc agcgaagcaa
    14761 aattcactga acgaatgaaa gaacaattga aagattgggg tatcaaagat gcaacgatcg
    14821 ttaatgcatc aggactgaat aactcttatc ttggcgaaaa ccgaccagaa ggtactggag
    14881 aaaacgacga aaatcaaatg tctgctcaag atgtggctat cgttgcccgt catttgatct
    14941 tggattttcc agaaatactg gatgtatcta gcactacgac acaaatgttt ggcgaaaaca
    15001 cgcaatcacc agtagaaatg gtcaactgga actggatgct cccaggattc gtgaattata
    15061 aagaaggcgt ggacggatta aaaactggta cgactgaact tgcaggagcc tgtttcgtcg
    15121 gtacgatcac taaaaacgat caacgaatca tcactgtcgt attgaatgca gacggccatg
    15181 cggaaaaccc aagtgcccgt ttcaatgaaa cagctcgctt aatggattat tgctacgaca
    15241 attggtcaga aaaagagctt ggaaaagcca atgcaagtat ccctagtcta aaaaccattg
    15301 atgtaaaaga cggaaaagaa tcaacagtgc ccgttgtgct taaaagtcct gtaaaagtat
    15361 gggtacggaa cgatatggat actggaaaat taacaatcac accaactctt gacaaaaaac
    15421 aagtcactga caatgagatc caagcaccta tggataaagg aacaaaaatc ggtaccgcaa
    15481 cgatcacctt ggcagaagat aaattaggtt atcttgaaga caacaacgcg ccatccacag
    15541 aaatcatcac ggataaaacc gtagaaaaag caaatatttt cgttattggc tggcgacgcg
    15601 tcgcagaatt tttcggtaac ttgttttaat tgaacaaaaa gaaaaaagca tctttccatg
    15661 tggaagatgt ttttttgctt tttctagttt aaataacagc ttttttcatc tattttcgtt
    15721 atactgtata agaaaaagga gttgaattta tgaatctaac cagagatctg atttttttgg
    15781 ttctctatct tattggggta ttctccatcg tttattggcg aaaacagaaa aaggatcctt
    15841 ccttgttctt ttacagcagt acatggacgt ttgcggtaat cttgacttat tttgtcgtgt
    15901 tggaaatttt ttattcaacc ttttattttt tgattccctt agtgtttttc agtatctttg
    15961 ccttttccta tttttcagaa aagcgaaggc tgttgaatgg tcttttattc aatattttcc
    16021 tcattagttt tggtatctac ttgtttgtct tgctatacga aacacaaaat atttttcttg
    16081 gcggactgat tgccttgatc acgatcccgt tactgcttgt ctttctattc ggcatttatg
    16141 gattgatcgt ttttcttttt tggaacggtg tcacagtttt acgtagagag tcgcattcat
    16201 tagctaatct tttaacgttg atactagcca tttttcttac attgtttctt gtttttgact
    16261 ttttcttgtt aaaatattta ccacaatgga taaatgcttt gtttttctgc atcccattga
    16321 tcttgatcta tctatttatc gtattctaca actttttgac tgtctcattc ttgtatcagt
    16381 tcaaccgccc tcgatacaac caagacttca tcgttgtgtt aggcgccggg ttgatcaatg
    16441 gagaaacagt ttctcctctt cttgccaagc gaatcaataa ggcgattgct ttttattggg
    16501 cacaaagccg tgcgacatta aatccgccta tcctattaat gtcgggtgga cagggagcag
    16561 acgaaaaagt accagaagca atggcgatga aacaatatgc catggaacaa ggaattcctg
    16621 aaagagatat tcttgtcgaa accaattcga caaccacttt ggagaatatg cgctattcaa
    16681 aggaaatcat ggatcagcaa atgaaaggtc cctatcgcgc gatcttcagt tccaacaatt
    16741 accacatttt cagagctggt ctctatgcac gacaagcgaa actgaaggca aatggcattg
    16801 gggcaaaaac agctttctat tacttgccta acgcgttttt aagagaatac atcgccatct
    16861 tgatgatgaa caaaaaaaga catatgatca tttgcggatt gcttttcgca ggttccgcat
    16921 tcttatcact gatcacttta cttttctaaa aaaacaaggt gtgacaaaac atatgtcaca
    16981 ccttgttttt ttcttttatt ctcttgacga ttggcaagaa tagtattcct tttttgacgg
    17041 ctacttgctg tgcagaataa ataccgctgt ttcctgaaca cataaagctg atcaagcaga
    17101 tcataaagaa gtaagcagca gcttgtgaac caaataattc gattcccatc acaaaacagg
    17161 caatcggtgt atttgtcgca ccagaaaata ccccgataaa acctaatcca gcaagaaaag
    17221 gaacagaaac atggagcaaa ggcgctagta ctgcgccaag cgttgcacca atctcaaata
    17281 gtggtgtcac ttctccccct tggtaccccg cacctaaaga caagaccgtg aagaataatt
    17341 tcccaataaa atcataagca tgtgcctgtc cattaaatga ttcctgcagt aacggaagac
    17401 tcaacccaag gtaacgttgt gtttgcaaaa tcaacgctgc ggctacaact actgctccac
    17461 ctatcgtatt tcgccaaaca ggatgaggaa tccattgcgc ataccgcgct tttaaaaaga
    17521 cgatcgaacg gctgaatacc caaccaatca aaccaaaagc aatcccagca acaaaaactt
    17581 tgaagaacag caacatactc cattcaggaa tcacacccat ttggtaatgt agatgagaaa
    17641 caccatagct ttctgtcatg aaatttgcaa aaagcgcagc aaagaagcta ggaaatattg
    17701 cttctgttcg gactttaccg attgccaaca cttctaatcc aaataatgtt ccagctaaag
    17761 gtgtgccaaa tacagaacta aaacccgcac tgattccact gataaccaaa atctcccgtt
    17821 ctgctttatc caatcggaat aaatgcgcta tattatcagc taatgctccc cccatttgaa
    17881 ctgctgttcc ttcacgacca acagatcccc caaataaatg ggtagtgatg gtcccaaaca
    17941 aggtcaatgg aatgagccgc aaagggatct tctcttctcc tccattccct tgatcgatca
    18001 ctaagttatt cccacgacta gaaagaccgc catgatacgc ataaagataa gcaaaaagcg
    18061 cgccgctgac tggcaataga taaagtagcc aaggatgtgc tagtcggata tctgtcacga
    18121 acgtcaggct atttaagaaa aaagcagaca agctccccat gaatactccg agaatcgctg
    18181 tgaccaccag ccacttcaac atgtacatca aaatacgcac tggactcggt aattgttttt
    18241 ccataaaaaa ttcctcctca aataaaaaaa cgcctactaa agaaaggggc tggccccaga
    18301 ctttagtagg cgtcattagc tatcttacgt tagcatttaa aggcgaacac catcacctat
    18361 tcacttccat taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttatttc
    18421 tttacacgtt cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattga
    18481 tcactatcat gacaaatata attccccttt ttcgtatacg tgccatcaga accggctttc
    18541 gttgtagaaa gaaagagcga gacattcgat gttttctgac agatcgcaca gattccctta
    18601 ttaatcgttg gtgataaagt accgctgatc ccttttaatt ttccatcttc gtaataaacc
    18661 atgaattttt tttgcgtccc tggatcattc caaccgatgt acgtatgtgc ttgcaagtcg
    18721 atttcattcc aagcgggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt
    18781 ggtttgctcg gctgcataaa cggctggacg atttgcttca gctcctccaa tgctcgttct
    18841 gcttgcgttt ttgtcagttt tacatctaaa actgtcgtta aaaacgtatc gatttctgat
    18901 ggttcgtaac ctaatacatc gcgaatcttt tcagctgcta gagcctgaac cgtttgtacc
    18961 gtttttgagt cattgacaga tgagtacgca tttaataact gctctacttc tctttttata
    19021 aaaaaatatt gatatggttt cattgtttgt tccatgattt taaagtcctt tcttctaacc
    19081 aaagtgcata ctttgtagaa aaaagtgatc ttctgcaaag tatgtgttac ctgaagatcc
    19141 cggactcttt taaataagat acgttttgca tcttatcatc acccccttta atgttcattc
    19201 ttctatgtac atgattcatt cgataaataa aagtaaaccg tatcttgagc agatagtcaa
    19261 ctgcaaacag aaaaaggact gtgacaaaag tcgtgtcaaa gtccctttct acaaacaaac
    19321 ggtgttccag aagtagcttc ttcaaaaaat aagccggaat ttcacaaaaa tttgagaagc
    19381 aattttcgta aattccttct tatttcttga agctgaccca cttctgtcac aacctcaata
    19441 tggtctatta ttggttttac ctgttaaatc gaggattctt ttccgaagta acgttgccat
    19501 ctcttacgca tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct
    19561 acgaatgtta cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg
    19621 tgcttcaaaa tgatatctcg ctcaagatcc gacaaatcgg tgatcttttc tgcattcttt
    19681 acagcgattc ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa
    19741 tcatagtaga acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc
    19801 catttttttg cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg
    19861 gaatgaacat gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct
    19921 tctacataag acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg
    19981 ccaccttttt gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga
    20041 ttattctagt cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa
    20101 aatgataaag aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca
    20161 tcgggatata tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt
    20221 cgttccccat gattattgcg aagttatctg atttttctac tttgtctaag gcgtgtgctt
    20281 cttcatttaa ttctgtgcca tacactgtaa accctttgtc tttcattaaa ggaatcactt
    20341 ctgtcaaatc tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca
    20401 cctttgtact ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg
    20461 catctgccgt acggatcatc gtccctacat ttccaggatc ttgtacatta tctaataaca
    20521 gccatcctcc tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc
    20581 cttgtggtgt aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag
    20641 aaatctcttt gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa
    20701 aaagttcttt tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca
    20761 aaagatattc ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga
    20821 ttcttggatt ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc
    20881 atggaatagg tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa
    20941 taggaaagaa aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa
    21001 ggcgtaggat ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg
    21061 gtaaaaaacg aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc
    21121 cagaaattta ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg
    21181 gatgtccaag aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt
    21241 tgtgaaaaag ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata
    21301 tttttcaaca ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcaataaggt
    21361 tgtgagaaag tcgcttagtt ttgagtagta aggagaattt tgtgaaaatg gctttccaca
    21421 tttttgcata aaatttgact taataccgag aaactgactt ttgaacaccg tcgataaggt
    21481 tgtttttgag acgctttgcc tcaagtatta aagataggta gtaaaaaagc tgatctataa
    21541 cttgcaataa atttgcttaa cccatatttt ccagacagaa caaaaaagtt tttagttttt
    21601 taaataagaa agaaacactt ggaatcactc ttttctttcc aagtatttct ttcttgattt
    21661 tttgtctttt tttcaaaatt tttcgctacg taaaagaacg aatatttata tgaaatttct
    21721 atgaagttga ttgaattcca tgcgcttttt tagtatagtt aagtttgtgt aaaaattata
    21781 gaagaggaag tattctatga ataaatggaa aaactggtta ttgggatccg gacttgtcag
    21841 tatcctgctc tttttatctg gctgtgtgcg gatggattca aacggaaacc ctgacacttc
    21901 gggaatcgtt tatcgagtac tcgtccatcc tatgggccaa gcgattacat atctagttga
    21961 gaatttcaat tggtcttatg gctgggcagt tattgcaatg actgtgatcg tccgtatcat
    22021 catcttgcca ctaggtatta gtcaatcgaa aaaaacaatg atccaatctg aaaaaatgca
    22081 agcgttaaaa ccacaagttg aagcagccca acaaaaattg aaagctgcaa caacacgtga
    22141 agaacaaatg gcagcacaag cagaaatgca gcaagtatac cgcgaaaacg gattgagtat
    22201 gaccggcgga atcggctgtc tgcctctatt gatccaaatg ccgatcttct ctgccttgta
    22261 ctttacggca cgttatacag aaggtattcg ggaatcatct ttctacggca tcgatttagg
    22321 ctcaccaagt atggtacttg ttgtcattgc aggggtcgcc tatctgcttc aaggatacat
    22381 ctcaacgatc ggtattcctg aagaacagaa aaagacgatg cgtacgatgc taatcgtctc
    22441 tcctgctatg atcgtcttca tgtcgatcag tgcgccagcc ggtgttacac tttactgggt
    22501 agtcggtggt gtgttcagct gtctgcaaac attcatcaca aacgttatga tgaaaccacg
    22561 tttgaaagca caagtggcag aagagttgaa acaaaacccg ccaaaacaag tcgtcactcc
    22621 aagaaaagac gtgacagaat cagcaaagcc agctaacaac aaacaagcaa caaaacaaat
    22681 caaacaacca aaaaacagta caaacaacaa aggccgcaac gcaggaaagc agcagaagcg
    22741 gaaataaaaa cataggacaa aaaagatgag agctaagccc tcatcttttt tgttactctt
    22801 cctcttctgc tattccatta attaattcgt tgacagagac aatgctttca catcctgcac
    22861 gaacaaactc gggctctgaa ccaatcattt gtgagttgat aaattcaatg accatcggaa
    22921 gattcatccc agcataaaga gtaatctgtt gtccttccat tattagttta gaaatgatat
    22981 ttgcaggtgt accacctaaa agatcgcata gtaccacaaa atcatttaac gaatctgtga
    23041 cagtgagaaa cttttgtttg tatgtttcag tgccatcttc tggtaataaa ggtaacgtgt
    23101 agatatcctc ttgaggcccc atgatcattt ctgtactttt tttcaattcc tcacaaaatt
    23161 ttccgtgact aatcaatacc aattgtctac tcatttttcg ctctccctta tttcgaaatc
    23221 acacccaatg cagataaagc aacacacaca attaatacga taaaaatcgc tcgagtagga
    23281 gtcatgtttt tctttcctaa taaccaataa acaccaccga cgatcccagc aggtaaaatt
    23341 ttaggtaaga tcatatcgat atagttttgc atattcaatg tcacatcgcc aatcgttggt
    23401 gcccatgaaa atttaacatt gatcattgtc gcaactaatg cccctaccat gaatacccct
    23461 aataaagtgg ctgcgtccgt taatgcagtt aagcgagatt gcatagtagt aactaaggaa
    23521 attccttctt tgtatgcaaa ttcaagttgc ttccaacgaa aaaccatgac aacgatctga
    23581 gcaaaaatcc atataaacac acctatcgga tttccatcag ttgccatatt cgccgccaaa
    23641 gccccaaaaa tcgttggaat caaagcagca aatacggaat ctccaatcgc tgcaaaggaa
    23701 cccatcaatc cagctttcag ccccgatacg gtttcctttg aagcaattcc ctctttttct
    23761 tccattgcaa gatcaatccc agtaatgatt gtattaaaaa aattagaagt attaaaaaat
    23821 tgcgtatgtg ttttcatcat ctcttttaat tctggtgtat tgtctccata tatttttctt
    23881 agttgtggca agatagtata taaatagcca gaaccctgca ttctctcata gttccaacct
    23941 aattgaaaag tgaacaaact tcttcgattg atttgcttaa aatcttcttt tgttaatttg
    24001 taattagatt tcgtcatctt cgatttcccc ctcaacagaa tgtgatggct cactagctaa
    24061 tggttgatgg tctttgtttt gatcattctt ataactgatc attgctagtg ctagaccaat
    24121 caatgctaca gcaagcatcg gcagcgaatt gaaagttgcc ggaagatctt taacaacgct
    24181 tgccacactt gttcctaata attgaacatt agagaataac gttgctaata atgccgtgat
    24241 cgtaaatcct aaaataagat aagatagatg tttttttact ggtaaatagc gcagaagaat
    24301 ggcaaaccca acagctggta aaacagctcc tgctactgaa agtccatcgc ctagccattt
    24361 tagattcccg tttaaaacag aaacaacatt ttcaactaat ccaccaccaa atgctaaagc
    24421 taaaaataca ggaatcatac gagagagcga ccaaggtaaa gctcccatta agaaattgcg
    24481 ttcgattgct tgataattca tgtcttctac gtgacggtca atacgatggg caaagtatgt
    24541 gttagcgaat ctagctaaaa tatccaattg gatcattaag cttgctacag gcactgcaat
    24601 agcagcaatc gcttgttctg gattcattcc aagagaaatc gagaaggcag ttgctaaaat
    24661 cgtcccagag ttggcatcta tttttgatgc gcctccaaac gtccccactc ctaaaactgt
    24721 caactgcata ctgccaccaa taatcaaacc tgcgtttaaa tcacccatta ctaaacctgc
    24781 atataaccct gcaaacactg gcgcactcat tgaagaatag atctgtaatt catctaaaat
    24841 ttgataacct gcatataatg ttaataataa gatctgccac cattgaatat ccatggatca
    24901 ttcctcctat ttgattaaaa gattcataaa gtcttctgct ttatcgtttg gtaccatttg
    24961 agcaattaaa tgtataccaa gctgatctaa ttttttaaat acttcgatat ctgcatctac
    25021 cacattgatt gattttgtaa tggaccgtgt ttcattcgtt tgtgacatat tccccacatt
    25081 tacttctttg ataggaactc ctttttctac taaagataag attcgatctg gcttttttgc
    25141 tacgatcaat actcgttgag agtcatattt gccagctaaa atatttgttg ctgctttttc
    25201 gattggcaaa atgcttaatt ttacccctgc tggagtcgct aacttcaatc cacttttttc
    25261 aattgtgttt gtggctacct catcatccac gaccatgatt cgactaatat tcagtttcgt
    25321 tgtccaaaga tttgctactt atccatgtat caaacgtcca tcgattctta caccaataat
    25381 tgccatcatg cattctcctt atctattttt tctatttatt gttcaattaa aactgtgaca
    25441 tcatcaaata tcccttctgt ttcaaagata acgttttcat tttttctttc ttatatttca
    25501 tttttaagct aagaacaaat tccttcccaa ctaaagaaaa cgctttcttt tatatttaag
    25561 ttcatttgat tttcttaact tgaatgtgtt tcatcaacat cgtaactttc atagggtcga
    25621 tccagccagt tttctcttgt tgcgcgtttg ccataccaca agccatagaa aatgcgagaa
    25681 tctcttcgat tgattgcctt tttgataacg catgggccga tcctgcaagg gtcgcgtcac
    25741 ctgaaccaac aggattaaca actgatattt taggaataat gacttcgtaa aaacactgag
    25801 caacttttgc cacagcacct ctctctccta aagaaacaac aacccatggc actccttcaa
    25861 atattggatg agctaataag gactgaattt tttctaattg aatcgaagaa cagatacgtt
    25921 ggccaatcag ttctcctaat tcttccagat tgggcttgat aagtagtggc ttctccttac
    25981 cttccaatac ttcaagcaaa aatttaccag aagtatctag taatatgtct ttcccttttt
    26041 tatcagctaa agcaaccaat tgcttatagt aatcaagagg gcatccttgg ggtaaactac
    26101 ctgagatcgt cataatatct gatatttcga ggagctgctc gaaatgatcg gtaaatgtct
    26161 gttgttcttt ctttgttatg aaggggcctc gttccaaaag ctctgtttgt ctcgaactat
    26221 gcaggatagc aatagaatcc cttgtctctc ctttaattgg aacaaactga tgcatgatcc
    26281 ctaactgatc tagctggttc tttagatatt caccgtgctg gccacctatt aaaccagtag
    26341 ctatcacctc ttctcctaat aaatttagaa ccctcgccac attcactcct tttcctcccg
    26401 ctgttttatt gactgtttca actcgattga ctttgtcgag gtttaattct tcaattgtat
    26461 acgcgatatc gatggaagga ttcaaggtaa ctgtcgtaat catttcatct tcaccttttt
    26521 gctttttgtt ttattgaacc aaggggtagc ggtttcattt attactttat ttaaagtctc
    26581 gatgttttct ctcccatttg ttcttaacca atgaatagct ccgtcttttc cattttcgac
    26641 gtatgctttg accccatctt tccatgtcgc acgtccacac aatacaccat tgaaggtgga
    26701 acccgattca tgagcaaaaa tcagtgtttg ttgaaataat tctgttgaaa cacccccact
    26761 caaaaaaatg aatggtaatg gagtcgcttc actttgctcc ttaaagaagg cagcagcctc
    26821 ttctttgctg tgaataaatt cacctgtgga atagccattc acataattca tattgactgg
    26881 tacttctact ttcaacacat caactttata ttttggatca gaaaattctt tcatcatctc
    26941 aataactttg tggggcttga ctttcgcata ctctggagaa gaactatcgc ttatttgtgc
    27001 gtcataagaa actaactcca aatagaaggg acgattttct ccttcgcact cactacctaa
    27061 acgttcgatg aacacatgct tcaaatgatt gatttcgggt gcttcatcaa catcgtagta
    27121 aagtaagaat tttactgcat ccgcttgctg ttctttcaat ctgagagaag accaatccgc
    27181 taataaatca ggtagacgac caggcgtagt tgcgtcatag ccggttttct cataagctaa
    27241 caataacccc gtctcttttg ctctgacttt tgctgctggc aatccatatt ccggatccaa
    27301 taaaatagca gaagcatagg gtgtcagctc agctgatact agttctttga attcactgat
    27361 ttggtcttca cttggctctt ttcccaacga tttgatcatt ttttttagag caccacgctg
    27421 atcgatcgct aatgcactaa taatcccatc ttttgttgat agtctctcca ttgccctatt
    27481 tttttcttca ttcatctgtc atttctcctt cggtttcata cttatgaata atgacgccct
    27541 tgactactcg attgaccgtg cctgttggag atggtgtatc tggtttatta gctactttga
    27601 ttgaagtata gagtgcaatg atttgtgcag tcataatcat cgctaatgca agataggcat
    27661 cgggcaattt actcaccgag tcttccaata gcacagtcat acctgagaaa tcaatagtac
    27721 ccggttgcgc aatcgcaatg acattggcag tgacccgatc ttcttttatt tcctctaaaa
    27781 tatctaagtc ataatttcgc gtatatggtt gattgctcac aaaacctaca acgagtgtct
    27841 cgctatttaa aaaagacttt ggtccatgac gaaaccccat cgaagaatca aaaattgtag
    27901 caactttacc tgctgttaat tctaaaattt ttaattgtgc ttctcttgtc gcacctgcca
    27961 aacttcctga acccagataa gcgatccgtt caaaaaaatc aggtaataac ttttgaattt
    28021 cttgatctct tgaaagaaga tcttgaccga ttttaactaa ttgttcaaga tatctttctt
    28081 ttttctccat tgctgtttga tcaaagatcc ataaagcact caataacata cacgtaaaac
    28141 ttcctgtcat agcaaaacct gcatcgtttg ctctctctgg catcaataac agataattct
    28201 gtttatcatc ctgtgcgttt tttgccaaca tcccctcttt agcacaagta atcgtcaaat
    28261 gaaacaatgt ttcaacttgt tcatttgcaa tttgcacagc cgctacactt tctggactgt
    28321 tgccactgcg agcaaatgaa actaataaag tcggttcatt tggaaacaaa aattcttttg
    28381 gtgaagctac aatatctgtc gtgccaaaac tgtggaagat aaaaaactga ttgtctccat
    28441 gttcatttag atagggtaag agcgtatccc caatatattg agacgttcct gcacccgtaa
    28501 aaataacacg tattcttctt ccgtttgctt ctttttttat ttgatcaaaa aatagagcta
    28561 aattttcttg gctttgcatc aatctatcga ttgtttcttt ccagagatct ggttgttgtt
    28621 ttatttcttg tgtcgtgatt gctgcaccta tttgttttaa tttgttcttc gatagctcaa
    28681 acatgttgtt ctccctctca atcattttcg gatatgccga attttataat gaaactggtc
    28741 cgctcttgct acactatctg tatattcgat aatctcattt ttcacgttat aggttatcct
    28801 cgtcagatgt aagacgggag cgccatcagc gattcgtagc acaacagcat cttttttttg
    28861 cacgatactt gcataaaact cttcatcagc taattgaact tgttgttcat aatcctgtga
    28921 aaacacatca tacaacggtt tactttcaag taacggcaaa tcaagtccca taaatagttt
    28981 cgccggtaga taagaacgct caaccatcaa tggctcttca tctgccaaac gcaatctttt
    29041 aagtttaaat acagtttcac ctaaatggat gttcaaattc ttagcaattc tcttattagc
    29101 ttccatttgt ctaaattcta aaatgaccgt cttagggacg cgccctattt cgcgcatttc
    29161 ttctgtaaag ctatatgctc cagctaagtt cgatgactta tcagacaaat cagagacata
    29221 ggtacctttt ccatgtcttc ggtaaatata accttgattt tccaattcct gtaaagccag
    29281 acgtacggtg gtacgactca ccccatagtg ttgacaaagt tctctttcag ataacagctt
    29341 atcatgcgga gtcatctgtg tttcgatacg atccttcaat aaatcaacta gttgataata
    29401 caatgatttt cctttatttg agctctccat aaaaatcacc tcttctttac tggttataac
    29461 cacaacacaa gaatagtgaa ttattaatca tttgtcaaag gttttttaga ccaaaaaagc
    29521 tattataaca gctctttaaa gctttatatc ttgaatagac aaagatacct caaaaatacc
    29581 agttaccaat aaaagaacag aaaaaaagac gaaccacata agcgatccgt caaaatggtc
    29641 taacttatcg agcagcgtga tccacattaa tccctatgat attcctacaa ctatcgtttt
    29701 attacttcca atatcattta caacaactaa aaatagctta ttaatgacaa atgttattat
    29761 tagttttaga taacttttgc agttttatac tttttttcta taaaatttga caaacatttt
    29821 tttaaataac taattatttt ttgaatctgt cactcagaca atcctttctc ttacttgtta
    29881 acataaatag aagttgctgt ttcttctaat agtgtactta attctttaat attttgtttt
    29941 ccttcttcta acatccaact actcgctttt tcttcgcctt cggtaacaaa aggtgctact
    30001 cccttcgccc atgttgctcg cccacataag acaccattga atgtcgaacc tgattgtttt
    30061 gcaaaacgca atgtatcttg aaatagtgca gcgctgactc ccgcacttaa aaaaataaac
    30121 ggttgttcag tcactttcga ttgttcatag aaatacgcgg atgcctctgc ttgactataa
    30181 caagtatctt gcccatatcc ttccacaaat ctcatattca ctggtacttc tactttcaat
    30241 acatccactt gataacgttc ttttgaaaat tctcgcatgg cttcaatcac tttatgcggt
    30301 tttacttttg catagtcaag agaagacgga tcgccaatag ttgcatcgta agaaaggagc
    30361 tctaaaaaga aaggtatgcc ttctgcttcg cattctgaac caatccgttc tataactatt
    30421 tgtttttgca cattaatctc tactggttca tcaacgtcat agtacaataa aaatttacaa
    30481 gcatccgcac ccacttcttt taaccttttc actgaccaac tattcagtaa atctggtagt
    30541 cggcctggca ttttcgcatc atagccggtt ttttcataag ctagcaacaa accacattct
    30601 ttctctcgaa gcgctgctgc aggcagacca tattctggat cgagtaaaat agcggaagca
    30661 tacggagtca ggtactgaga aaccatctgt ttaaattgga tgatatcttc tgatgtagct
    30721 tctttatatt ggttgatcat tttcttcaaa gcaccacgtt ggtcgatagc tagcgcgcta
    30781 ataattccag cttcatccgt caatcgcttc aaacgttcct gtttttctat actcattttc
    30841 ttcatatatc ttcacctatt tctttagtta atattgtagc tatttctatt tttgattttg
    30901 cagctctcaa ctgttctgta acctcttttc gcatcaatga tcgtgcaacg agagacaata
    30961 atttcaaatg agtcgttcct gcttctgaat ctgggataaa taacgcaaaa ataaaatcaa
    31021 ctgactgtct atctaaactg ttccattcaa ttcctttttt cagtgtgacg ataatcaaac
    31081 taggttcatt gattgtttga gacttcgcat gagggatagc aaaacctgac attattcctg
    31141 tagtaccttc tttttctctt tccagaagtt tttgatatac ttctttttcg tcatgagcga
    31201 ttcctaaaca gaatgtttgc tttgctaaaa acttaaatgc ttcttcttga ttatctaatg
    31261 gctgttgtaa aaaaatgttc tcttcatgaa tgattgtcat gataaacctc ctgctcttcc
    31321 ttcttcgata actgtacgat ttgttgcttt attatccctc ttcttgtctt tatcagttac
    31381 tcttttcttt tggatcaaac ctagtatcac tccaccaacg atcgaaccaa taagaatcgc
    31441 tgtaacccat tggatttttc ccgtcactac aggtaacact aaaaaaccgc catgtggggc
    31501 tggtacttgg acaccaaaca gataggtcaa gacagcagca attgatgagc ctaacatcat
    31561 cgttggtaat actcggatcg ggtctttcgc agcaaacgga atagctcctt ccgtaatatg
    31621 agttgatcct aaaataaaat taaccaaacc tgcattacga tcattagtat caaaatattt
    31681 accaaacaat aaagtagcaa aaccagtaat caacggtggt gcaatgcaag ctgctgacgc
    31741 accagccata aaagtcgtgt ttccttgtgt caaaagagct gttcctgtta catatgccgc
    31801 cttattaacc ggtcctccca tatcaaacgc acacatacag ccaacaatca cgccaagaac
    31861 aagtggattc gaatattcaa atccagctaa aaagctcatc attccttcat ttatagcctt
    31921 cattggacct gccagcaaag acatcacgat gccaataaat gctacaccaa taactggata
    31981 taagacgatt gccttcaagc catctagttg tttaggtaaa attatcagta gcttttgcaa
    32041 aaataagata aaataacctg ctaagaaccc agctaaaata cctcctaaaa atccagtacc
    32101 gccattcatg gctacgattc caccaacaaa tccaacgact aaccctggac gtttcgcgat
    32161 cccttctgca atataagctg ctaaaactgg aaccatcatt cccattgctg catcgtcaac
    32221 tgcttttacc attgccgctg tagcattgta ttgtgcatga tctggatcag aagaatagat
    32281 tccccaaacg gcaaatgaaa tagcaatcaa tactccgcca gctaccacga aaggcaacat
    32341 atgagagaca ccgttcatca aatttttata gatgctacga ccaaaattag caccacttat
    32401 cgcttctggt tctaaagtat cagcagcttc attatctgct aagacagtac cttttcctgc
    32461 caatgcttcg tcaatcagcc gttgtgcatc cttgattcct gtactgacag aaacatcaat
    32521 gactcgtttt cccacaaatc tttctgaatg gacatctttg tctgcggcaa taatgacagc
    32581 atctgcctgt ttgatttctt cagccgttaa ttcattctcg atcccaattt gtccatgggt
    32641 ctccactttg atcgtgatgc cttttttcct agctgcttgc tctaatgcct cttgcgccat
    32701 gtaggtgtgc gcaattcctg tcggacaacc ggttgctgca attagttgat attttcccat
    32761 ctttcgaacc tctcttcctt gattttgatt tgtttactta attcctccac atccaaaaaa
    32821 tcagtcagtc ctttacgaaa agcagtagag ctgcctgctg ctatgctttt acgtaacgtt
    32881 tcatcaagcg atcgtcgatt catgtatcct gctaaaaaag tgcctaacat ggcatcacct
    32941 gcacatgctg tattgacaac ttttcctttt ggcgagtttc ctcgaatcac cctgtctttc
    33001 gtaaaaagaa tcgctccttt ctcaccgagg gataacaaaa cattttctgc gcctgactct
    33061 actagtcgtt gtccataaac aaagtaatct tcatctgtaa ccatttttcg tccgaaccaa
    33121 gaagaaagtt cttcctcatt tggtttcagc aaaaacggtc gatagggtaa acaatccatg
    33181 acttcttgat cactactatc aataatcaaa caaatccgac tgatttctat taaaaccttt
    33241 ggagacagcc ctcttggtaa actcccagaa acacaaagat aatcccctgc ttgtaatgct
    33301 cttatttgat ttaaaaaatt atgaacagct gtctgcggta tttcaggtcc ttgattcact
    33361 aatttgtatt cttctcccgt ttgattgact tgtgtaaata cattgatacg cgtcatacca
    33421 ggcacttcaa taaactctgt ttggatttgt ttctgctgta aatagtcttc tatatacttt
    33481 cctgtgaacc cagcagaaaa acctaatgcc gtacttggga aatctagcat ctttaatact
    33541 agtgacacat tcaccccttt tccgtttgcc tgaatgtcat cctcttttgt gcggtttacc
    33601 ataaaaggat gcatctcatc tgtttcaata aatagatcaa ttgccaaatt catcgtgcat
    33661 gtgtagatag acataaccaa cgacctcctt gattctatta tgaaatacgc gaaatagaaa
    33721 atgttttctt aatcgtccca acacaaagaa aacctttaca tttataaact cgtcgtgctt
    33781 tttttaacta acttttggtg aaagaaggaa agaatatgaa tcacaaatta agtgaaacag
    33841 agcagtacct ctggaacttt atcgaacacc tcatcctaga gatccctaac tattccatcg
    33901 tgaaactaag tgaacaagca aatgtctcaa cagccaccat tgtgcggaca atgaaaaaga
    33961 aaggctatga agggtttact tcgtttaagc atcatctaaa agaaggagaa aacaagaaca
    34021 tcaatttttc atttttagat aaagtagaca aaggaatccg ccgagccatt ctaaaaaatg
    34081 aacaagaagt ggtacgtaca atcaatatgt tggaaatcgg gaacattgaa gatgcaatac
    34141 aaagaatcaa atttgctgac cgggtttttg tctttgcccg aggattttca gaaatgattg
    34201 gccaagaaat gctggtaaaa cttcaattaa cgggaaaaaa ttgtcagatg catactgatc
    34261 cagaaattat caaaagcatc agtcggaaac taactaagaa agacagcgtt atttttgttt
    34321 ctttgaacgg tgagaaaaca aataaaaaca tcaaaaataa gatttatgtc tttatttttc
    34381 aattttttaa at
//
LOCUS       DAZUKT010000025        33995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000025 DAZUKT010000000
VERSION     DAZUKT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.025"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACG816_001176"
     CDS             complement(27..1259)
                     /locus_tag="ACG816_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4920065.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACG816_001177"
     CDS             1514..2083
                     /locus_tag="ACG816_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4920066.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACG816_001178"
     CDS             complement(2261..2701)
                     /locus_tag="ACG816_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4920067.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACG816_001179"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACG816_001179"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4920068.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACG816_001180"
     CDS             3655..4578
                     /locus_tag="ACG816_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4920069.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACG816_001181"
     CDS             complement(4803..5042)
                     /locus_tag="ACG816_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920070.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACG816_001182"
     CDS             complement(5047..5466)
                     /locus_tag="ACG816_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4920071.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACG816_001183"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACG816_001183"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS4920072.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="ACG816_001184"
     CDS             complement(7038..7673)
                     /locus_tag="ACG816_001184"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4920073.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="ACG816_001185"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="ACG816_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="ACG816_001186"
     CDS             complement(8015..8650)
                     /locus_tag="ACG816_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4920074.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="ACG816_001187"
     CDS             9162..10304
                     /locus_tag="ACG816_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4920075.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="ACG816_001188"
     CDS             10297..10503
                     /locus_tag="ACG816_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4920076.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="ACG816_001189"
     CDS             10582..11775
                     /locus_tag="ACG816_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4920077.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="ACG816_001190"
     CDS             11980..13119
                     /locus_tag="ACG816_001190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4920078.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="ACG816_001191"
     CDS             13112..13912
                     /locus_tag="ACG816_001191"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4920079.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="ACG816_001192"
     CDS             13912..14739
                     /locus_tag="ACG816_001192"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4920080.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14744..15451
                     /locus_tag="ACG816_001193"
     CDS             14744..15451
                     /locus_tag="ACG816_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4920081.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="ACG816_001194"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="ACG816_001194"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4920082.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="ACG816_001195"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="ACG816_001195"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4920083.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="ACG816_001196"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="ACG816_001196"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4920084.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="ACG816_001197"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="ACG816_001197"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4920085.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="ACG816_001198"
     CDS             19036..21069
                     /locus_tag="ACG816_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920086.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="ACG816_001199"
     CDS             21113..22393
                     /locus_tag="ACG816_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4920087.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="ACG816_001200"
     CDS             22603..23409
                     /locus_tag="ACG816_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920088.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="ACG816_001201"
     CDS             23421..24641
                     /locus_tag="ACG816_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920089.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="ACG816_001202"
     CDS             24631..26217
                     /locus_tag="ACG816_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4920090.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="ACG816_001203"
     CDS             26256..28394
                     /locus_tag="ACG816_001203"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4920091.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28762..29745)
                     /locus_tag="ACG816_001204"
     CDS             complement(28762..29745)
                     /locus_tag="ACG816_001204"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4920092.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30123..31514
                     /locus_tag="ACG816_001205"
     CDS             30123..31514
                     /locus_tag="ACG816_001205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4920093.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31530..32570
                     /locus_tag="ACG816_001206"
     CDS             31530..32570
                     /locus_tag="ACG816_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4920094.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32589..33674
                     /locus_tag="ACG816_001207"
     CDS             32589..33674
                     /locus_tag="ACG816_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4920095.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33707..>33995
                     /locus_tag="ACG816_001208"
     CDS             33707..>33995
                     /locus_tag="ACG816_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4920096.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGA"
BASE COUNT        11017 a         5825 c         6701 g        10452 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accatttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa
    28801 agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt
    28861 cttagcgtca atataaagaa catttccact attaccaatt gctgaatata tttcgttctc
    28921 agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact
    28981 ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata
    29041 catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa
    29101 accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga
    29161 cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt
    29221 tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact
    29281 tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc
    29341 ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga
    29401 acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg
    29461 cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata
    29521 atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag
    29581 caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc
    29641 attccattca tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt
    29701 tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac
    29761 tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta
    29821 atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata
    29881 aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc
    29941 aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag
    30001 aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc ttataaacta
    30061 ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat
    30121 atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg
    30181 ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc
    30241 cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg
    30301 cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt
    30361 ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg
    30421 gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt
    30481 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
    30541 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
    30601 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
    30661 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
    30721 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
    30781 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
    30841 aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
    30901 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
    30961 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
    31021 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
    31081 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
    31141 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
    31201 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
    31261 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
    31321 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
    31381 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
    31441 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
    31501 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
    31561 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
    31621 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
    31681 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
    31741 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
    31801 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
    31861 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
    31921 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
    31981 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
    32041 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
    32101 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
    32161 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
    32221 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
    32281 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
    32341 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
    32401 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
    32461 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
    32521 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
    32581 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
    32641 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
    32701 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
    32761 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata
    32821 agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa
    32881 ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa
    32941 ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat
    33001 tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt
    33061 ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa
    33121 attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg
    33181 tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt
    33241 tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta
    33301 gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag
    33361 gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc
    33421 tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct
    33481 tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag
    33541 atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa
    33601 cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag agtatatata
    33661 gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga aaatatgtta
    33721 tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc gattgaacga
    33781 aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat ttattgaagc
    33841 atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta aaatttattg
    33901 gggtggtttt tccattgtac aagcagaaat caatttagta aagagagcat tacaaaatga
    33961 gaaatattta aaatatgtat tattatctgg tgctg
//
LOCUS       DAZUKT010000026        31896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000026 DAZUKT010000000
VERSION     DAZUKT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.026"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACG816_001209"
     CDS             complement(26..601)
                     /locus_tag="ACG816_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4920097.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACG816_001210"
     CDS             complement(606..1580)
                     /locus_tag="ACG816_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS4920098.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACG816_001211"
     CDS             1863..2831
                     /locus_tag="ACG816_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4920099.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACG816_001212"
     CDS             2928..3221
                     /locus_tag="ACG816_001212"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4920100.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACG816_001213"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACG816_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4920101.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACG816_001214"
     CDS             3678..4433
                     /locus_tag="ACG816_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4920102.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACG816_001215"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACG816_001215"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4920103.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACG816_001216"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACG816_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4920104.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACG816_001217"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACG816_001217"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4920105.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACG816_001218"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACG816_001218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4920106.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACG816_001219"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACG816_001219"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4920107.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACG816_001220"
     CDS             9170..9712
                     /locus_tag="ACG816_001220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4920108.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACG816_001221"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACG816_001221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4920109.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACG816_001222"
     CDS             11305..12207
                     /locus_tag="ACG816_001222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4920110.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACG816_001223"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACG816_001223"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4920111.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACG816_001224"
     CDS             13650..14072
                     /locus_tag="ACG816_001224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4920112.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACG816_001225"
     CDS             14541..14771
                     /locus_tag="ACG816_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4920113.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACG816_001226"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACG816_001226"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4920114.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACG816_001227"
     CDS             16182..16358
                     /locus_tag="ACG816_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4920115.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACG816_001228"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACG816_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4920116.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACG816_001229"
     CDS             16853..17320
                     /locus_tag="ACG816_001229"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4920117.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACG816_001230"
     CDS             17405..18289
                     /locus_tag="ACG816_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4920118.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACG816_001231"
     CDS             18403..18639
                     /locus_tag="ACG816_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4920119.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACG816_001232"
     tRNA            18727..18807
                     /locus_tag="ACG816_001232"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACG816_001233"
     tRNA            18814..18885
                     /locus_tag="ACG816_001233"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACG816_001234"
     CDS             19011..19181
                     /locus_tag="ACG816_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920120.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACG816_001235"
     CDS             19401..20108
                     /locus_tag="ACG816_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4920121.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACG816_001236"
     CDS             complement(20167..20811)
                     /locus_tag="ACG816_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4920122.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACG816_001237"
     CDS             20938..21225
                     /locus_tag="ACG816_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920123.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACG816_001238"
     CDS             21333..21653
                     /locus_tag="ACG816_001238"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4920124.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACG816_001239"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACG816_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23763
                     /locus_tag="ACG816_001240"
     CDS             23044..23763
                     /locus_tag="ACG816_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS4920125.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="ACG816_001241"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="ACG816_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4920126.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="ACG816_001242"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="ACG816_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4920127.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="ACG816_001243"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="ACG816_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4920128.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="ACG816_001244"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="ACG816_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4920129.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29209..30093
                     /gene="ftsX"
                     /locus_tag="ACG816_001245"
     CDS             29209..30093
                     /gene="ftsX"
                     /locus_tag="ACG816_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4920130.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="ACG816_001246"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="ACG816_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4920131.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10607 a         5565 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DAZUKT010000027        31039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000027 DAZUKT010000000
VERSION     DAZUKT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.027"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(392..1975)
                     /gene="abc-f"
                     /locus_tag="ACG816_001247"
     CDS             complement(392..1975)
                     /gene="abc-f"
                     /locus_tag="ACG816_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4920132.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2080..2208)
                     /locus_tag="ACG816_001248"
     CDS             complement(2080..2208)
                     /locus_tag="ACG816_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920133.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2553..2696)
                     /locus_tag="ACG816_001249"
     CDS             complement(2553..2696)
                     /locus_tag="ACG816_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4920134.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2919..3398)
                     /locus_tag="ACG816_001250"
     CDS             complement(2919..3398)
                     /locus_tag="ACG816_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4920135.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3471..3755)
                     /locus_tag="ACG816_001251"
     CDS             complement(3471..3755)
                     /locus_tag="ACG816_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920136.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3833..4027)
                     /gene="ssrS"
                     /locus_tag="ACG816_001252"
     ncRNA           complement(3833..4027)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG816_001252"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4099..5385)
                     /locus_tag="ACG816_001253"
     CDS             complement(4099..5385)
                     /locus_tag="ACG816_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4920137.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5523..6194
                     /locus_tag="ACG816_001254"
     CDS             5523..6194
                     /locus_tag="ACG816_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4920138.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6160..6648
                     /locus_tag="ACG816_001255"
     CDS             6160..6648
                     /locus_tag="ACG816_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4920139.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6661..7608
                     /gene="prmA"
                     /locus_tag="ACG816_001256"
     CDS             6661..7608
                     /gene="prmA"
                     /locus_tag="ACG816_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4920140.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7609..8373
                     /locus_tag="ACG816_001257"
     CDS             7609..8373
                     /locus_tag="ACG816_001257"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4920141.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8606..10819
                     /locus_tag="ACG816_001258"
     CDS             8606..10819
                     /locus_tag="ACG816_001258"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4920142.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10830..11276
                     /gene="dtd"
                     /locus_tag="ACG816_001259"
     CDS             10830..11276
                     /gene="dtd"
                     /locus_tag="ACG816_001259"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4920143.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11447..12706
                     /locus_tag="ACG816_001260"
     CDS             11447..12706
                     /locus_tag="ACG816_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4920144.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12795..13742)
                     /locus_tag="ACG816_001261"
     CDS             complement(12795..13742)
                     /locus_tag="ACG816_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4920145.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14136..15428
                     /gene="hisS"
                     /locus_tag="ACG816_001262"
     CDS             14136..15428
                     /gene="hisS"
                     /locus_tag="ACG816_001262"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4920146.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15443..17212
                     /gene="aspS"
                     /locus_tag="ACG816_001263"
     CDS             15443..17212
                     /gene="aspS"
                     /locus_tag="ACG816_001263"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4920147.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17321..18196
                     /locus_tag="ACG816_001264"
     CDS             17321..18196
                     /locus_tag="ACG816_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4920148.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18462..20345
                     /locus_tag="ACG816_001265"
     CDS             18462..20345
                     /locus_tag="ACG816_001265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4920149.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20420..20704)
                     /locus_tag="ACG816_001266"
     CDS             complement(20420..20704)
                     /locus_tag="ACG816_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4920150.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20694..21428)
                     /locus_tag="ACG816_001267"
     CDS             complement(20694..21428)
                     /locus_tag="ACG816_001267"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4920151.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21557..22180
                     /locus_tag="ACG816_001268"
     CDS             21557..22180
                     /locus_tag="ACG816_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4920152.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQVAFDKLDKEVNPDFKYEVKKK"
     gene            22372..23493
                     /locus_tag="ACG816_001269"
     CDS             22372..23493
                     /locus_tag="ACG816_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4920153.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23500..26628
                     /locus_tag="ACG816_001270"
     CDS             23500..26628
                     /locus_tag="ACG816_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4920154.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26647..29841
                     /locus_tag="ACG816_001271"
     CDS             26647..29841
                     /locus_tag="ACG816_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4920155.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29987..30694)
                     /locus_tag="ACG816_001272"
     CDS             complement(29987..30694)
                     /locus_tag="ACG816_001272"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4920156.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10122 a         5626 c         6567 g         8724 t
ORIGIN      
        1 aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta acttcaattt
       61 cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt caaaagggaa
      121 aagactggca ttttatttat tgattttcag gaaaatccca atattttatt ttcattcatc
      181 tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc ctcgtgggct
      241 gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca ttcattgatc
      301 gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag cacaggtatg
      361 aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg agcggctggc
      421 ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc aaaaagtagc
      481 ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct tcaatttctt
      541 ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg ttgatttttt
      601 ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa tctttgatcc
      661 gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga gcgacttctt
      721 cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg gcaaactgca
      781 gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg ccaccgtaag
      841 gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca ttgtcgccga
      901 tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat agtacttgat
      961 cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat tttccaccat
     1021 tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt gtcagtttcg
     1081 ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct ccttcatgtg
     1141 cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt tgctgcagtt
     1201 cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa cggctgtagt
     1261 ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg gtgacttgat
     1321 cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc tgtaaatagt
     1381 tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg agtaaaagta
     1441 aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg cctccgctaa
     1501 gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag atcgctgcaa
     1561 tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc tcaccgtaac
     1621 gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg tagcgactca
     1681 tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg ttgacggtcg
     1741 cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt ttgcgactga
     1801 taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt gatttgccag
     1861 ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag gataaatcct
     1921 caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg atcatgatct
     1981 ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta tttaaactca
     2041 gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt caactgtttt
     2101 ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa atgcgatatg
     2161 gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag tattttccct
     2221 ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca actagcgctg
     2281 acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt atctgactga
     2341 gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt caggcgaaat
     2401 catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt tgcttaatac
     2461 cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc aggagtatcg
     2521 ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt accgaatgta
     2581 gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc gataaaatcg
     2641 gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac ttccacattg
     2701 acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg cgactaatcg
     2761 ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc ctttatttta
     2821 aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg gttttttgtg
     2881 caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt gtggtcttta
     2941 gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc tgcgtttcta
     3001 ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa tcctgtggct
     3061 cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat aatttgtttt
     3121 ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt cgcattgctt
     3181 tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag cattccatca
     3241 aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag aagttctccg
     3301 ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc atcagaacca
     3361 tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc ctcaagtcaa
     3421 tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat ttactgcttt
     3481 ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat aattacgatc
     3541 agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat acccattacg
     3601 cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt atcatttttg
     3661 ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac gaataataat
     3721 actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa aataagcaac
     3781 aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga tggtaatctc
     3841 cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag gtgggtccca
     3901 tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac acaagacggg
     3961 tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc gaacatcaca
     4021 gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga gctcgcagaa
     4081 acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt ttatacggta
     4141 atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat aatactgagc
     4201 attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa aatgatgagg
     4261 atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc tatctctcaa
     4321 atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg catctaatgc
     4381 catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg
     4441 gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca ctgtacgagc
     4501 cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca gacgccggca
     4561 aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg cagcatccac
     4621 atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag catctccatt
     4681 tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca gtgtaagatg
     4741 aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc cattcaatgc
     4801 actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt cgtctaactg
     4861 gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga ctgccaactg
     4921 gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc ggatcgctgg
     4981 attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc ggccactttc
     5041 taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt catccaataa
     5101 gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat ccttttttga
     5161 atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga tcgcacttgc
     5221 gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga gcatgcgagc
     5281 atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac caacgacttc
     5341 atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat tttcctcctt
     5401 cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa gaacagcctt
     5461 ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag taggtgagaa
     5521 aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg ccagaagtgg
     5581 cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga ttagctggat
     5641 atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat agctatggtt
     5701 taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc tacctttaca
     5761 cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt cctcaaggag
     5821 taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg
     5881 gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca ttagcaattc
     5941 ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt tttgagaaaa
     6001 aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat
     6061 ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa
     6121 aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta
     6181 acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc tttagattgg
     6241 gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa
     6301 tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga atttgaagac
     6361 ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta tctagcagta
     6421 atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt agtggaatat
     6481 ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga tgaagacgat
     6541 gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt agtagaagta
     6601 aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg agtgacagat
     6661 atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc agttgctaat
     6721 attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga tgttgaacat
     6781 tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc gctgaaagaa
     6841 ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat cttgcctttt
     6901 atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa aaataaagta
     6961 acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata ctatcatccg
     7021 gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca gacagaagat
     7081 cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg cactcatccc
     7141 acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga aacattgcta
     7201 gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat
     7261 gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg
     7321 aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg aatcgagatt
     7381 gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat gatcccagat
     7441 gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat tgaagagaag
     7501 aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca aattttccag
     7561 caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat gcagcggtat
     7621 tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga aaactatcac
     7681 cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc attcaatgat
     7741 caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta tctgaaagaa
     7801 attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc gagtggctat
     7861 cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg agcgcaagct
     7921 tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa
     7981 cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca tcggcagatc
     8041 gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct agatgattat
     8101 gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc
     8161 aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg accagaaggt
     8221 ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact ttgtggatta
     8281 ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc tgctagctat
     8341 caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa agagtatcga
     8401 cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat ttatacaatg
     8461 gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa aaaaacgtat
     8521 aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc cgttttaccg
     8581 aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg gacccggtgt
     8641 cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg agaaagcatg
     8701 cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac cctacattat
     8761 tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc atacagtggc
     8821 tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag atctgagaga
     8881 agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag ggaaaataaa
     8941 atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc tagcaatggc
     9001 tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca tgcgtacgtt
     9061 gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag aaatctatgc
     9121 gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag atacagcact
     9181 tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga cgaagagaga
     9241 agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga cagaagagct
     9301 ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct atcgtaaaat
     9361 gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc gagtgatcgt
     9421 agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat ggaaaccaat
     9481 gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc aatctttgca
     9541 tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa cacaggaaat
     9601 gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag gaaaaaatga
     9661 aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga tactcgaact
     9721 tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag atatctttag
     9781 tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa aaggatccgg
     9841 accattggac tttgcataca gtatccatac agatatcggt aacaaaacca ctggtgcaaa
     9901 agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata tcattgagat
     9961 catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag ttgctactag
    10021 caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag aaaatgtgat
    10081 caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc ctaaagatat
    10141 tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa cagaagatga
    10201 tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca atcgtctgac
    10261 tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag aagaaatcat
    10321 gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat
    10381 tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga ttcctggtga
    10441 tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc gagattgtcc
    10501 gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg aatgggaaga
    10561 tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct ataatcgttc
    10621 aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt
    10681 tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg gtatccagaa
    10741 tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg tctacagtgt
    10801 acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg gtaagtaaag
    10861 ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta cttgttttat
    10921 taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa aaaattgcac
    10981 aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag
    11041 gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac
    11101 caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa tcattcaatg
    11161 aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca gatatgtcgg
    11221 tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac aaataaagta
    11281 atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact gataaactct
    11341 aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag
    11401 ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga aagaagtacc
    11461 agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat acaacatgtt
    11521 ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga
    11581 agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa ggaatttcaa
    11641 acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag gtggagtaca
    11701 gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc taatgacacc
    11761 ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag cacttgaagc
    11821 acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta catctatttg
    11881 cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa aattagcaga
    11941 atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca taaaaggtgt
    12001 cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa
    12061 tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca cagatttaga
    12121 tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca tccgtccaac
    12181 ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta gaccagattt
    12241 gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc aagaaatgag
    12301 acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag tagaagtaga
    12361 tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa cagaccatga
    12421 tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa
    12481 agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa attttcgaca
    12541 aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt tttccgatca
    12601 gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc cattcaatca
    12661 gtttccgact tatccgaata gccgagcgct actttacaag aattagatta gcataaactt
    12721 atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta ctataaattg
    12781 ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata atcttcattg
    12841 gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg cttagctcca
    12901 ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag ttgcgcagga
    12961 gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa aatttctctg
    13021 aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc aaattgtttt
    13081 tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa atagggaaca
    13141 aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg ccgatggatc
    13201 aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc tttcaattgt
    13261 tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt taattcgtgc
    13321 aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg
    13381 tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt gagaaactca
    13441 ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt agcgatgacg
    13501 acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc ttcggaacgg
    13561 ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa agctgtacga
    13621 actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca
    13681 tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct tatttctgcc
    13741 atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct gaactaaaac
    13801 gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt ttagtaggtt
    13861 ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt acaagagagg
    13921 atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct ggagatggac
    13981 gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa
    14041 attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca agggtgattt
    14101 ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga ccaaaaggaa
    14161 caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt
    14221 tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa cactatgaag
    14281 tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg tatgattttt
    14341 atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca atcgttcgtt
    14401 cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa actttttata
    14461 tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa
    14521 tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc atggtgatgg
    14581 cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc aattcattag
    14641 gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt ttaccttttg
    14701 aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta cgcgtattgg
    14761 acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc ttagattatc
    14821 taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat gcattggctg
    14881 ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca catacgattt
    14941 ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac
    15001 gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc ggatttgcaa
    15061 tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc cctgcattta
    15121 atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag
    15181 tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg aaccgtaaag
    15241 caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg gtgatcggtg
    15301 aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa
    15361 gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa atgactttat
    15421 tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat tagtttccga
    15481 acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac gagaccttgg
    15541 gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc
    15601 tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga
    15661 agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa tggccactgg
    15721 tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt
    15781 tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc gttatttgga
    15841 tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa caaaagcgat
    15901 tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc tagggaaatc
    15961 tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg gacatttcta
    16021 tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg gattcgaccg
    16081 ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc gtcagcctga
    16141 atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc aaacttacac
    16201 agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca cattgccatt
    16261 tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac cggatacacg
    16321 ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat tcaaagtctt
    16381 ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga
    16441 tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat tcggagctaa
    16501 ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg ctaaattcat
    16561 gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg acttgctgat
    16621 gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt
    16681 aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg taactgattg
    16741 gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc atccattcac
    16801 gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag tctatgcaga
    16861 agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc gtattcatac
    16921 tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac aaatggaaga
    16981 acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg gcggtattgc
    17041 attaggattg gaccgttttg caatgttatt agccggagaa gataatattc gtgaagtcat
    17101 tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc
    17161 gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat agccgataaa
    17221 aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca
    17281 ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa
    17341 ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg cgttgatcgc
    17401 atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg gtattactgg
    17461 tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag taccggtatc
    17521 aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa aaatcgggaa
    17581 gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta tccatatttt
    17641 accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg gggctgtgat
    17701 gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc tagatatcgt
    17761 gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga tctacttcaa
    17821 tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt tttacagtac
    17881 gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat
    17941 gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc aaaactgtat
    18001 gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg ataagcagac
    18061 agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag caatgaaagc
    18121 ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg gtcgtttcta
    18181 tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg tagacagacg
    18241 ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa ctgctttttt
    18301 atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt tatgggatga
    18361 tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt aatacccgta
    18421 tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt
    18481 taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct gtggtgggtg
    18541 ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc gttccgattt
    18601 ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg
    18661 gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat ccggcacttg
    18721 tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg ctgttaaaca
    18781 atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg caaatcaata
    18841 acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat atctggtctt
    18901 tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg ttgatggtat
    18961 ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc ctctccgcca
    19021 tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat tacggtaccg
    19081 acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc
    19141 gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct ggaggaatct
    19201 cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat ttcatctatt
    19261 acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg acagcacacc
    19321 ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt ggcaaacgaa
    19381 gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca aaggtgactg
    19441 atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt ctaatcatca
    19501 gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat ggtcgtacat
    19561 tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa ccatgggttg
    19621 ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca ccgaaaaacg
    19681 ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa aaattagcag
    19741 aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg atccttgata
    19801 agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta acggcatttg
    19861 gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca aaagtagtgg
    19921 tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa aaattaaagg
    19981 aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc tcattctcgg
    20041 aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg
    20101 tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga
    20161 aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac catagtgact
    20221 ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac attcacttag
    20281 tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca aacgtgacag
    20341 aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag tttttttata
    20401 tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag tgtgtctgta
    20461 aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct tttgttgctt
    20521 cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt ttttgaagac
    20581 gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa agatcggttg
    20641 tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa tgttcatttg
    20701 ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta agtgatcaat
    20761 ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac taacaaaata
    20821 tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc aattcgatag
    20881 tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat agtaaagcgt
    20941 ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt cgtatgaatc
    21001 tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg cagccctttg
    21061 aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac agataagctg
    21121 tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag cgcgatacta
    21181 cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc cgcctgggtt
    21241 cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac aatcttccct
    21301 tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa agaaaacact
    21361 ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg
    21421 tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac tatttttatt
    21481 aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg accataagaa
    21541 gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag tactattagc
    21601 cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag atgagaatta
    21661 catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg tagctgcagc
    21721 acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag ttctgcgatt
    21781 tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac caagcgtcat
    21841 taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg
    21901 gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa tgtctaaagc
    21961 gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt tcaagcgtca
    22021 gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga aaacggataa
    22081 agaaggtatg gcggagatcg aacgacgaac acaagtagct tttgacaaat tggacaaaga
    22141 agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg
    22201 gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa acgagggaga
    22261 gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt
    22321 aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg aatgcgtttt
    22381 ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt gattgaagac
    22441 cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt agaggcaatc
    22501 gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt agaattatac
    22561 aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc tatatcagga
    22621 aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta ttcgaactat
    22681 tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa cgtacaattt
    22741 ttctttcttc cttactttga accaattgca gcgagattat attttgaaga tgacaatatc
    22801 cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct atttgattca
    22861 gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa atctgattcg
    22921 gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact tacaccgttt
    22981 gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga taatgctcgc
    23041 tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa aggcgtatgg
    23101 atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc cctgcatgat
    23161 attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc
    23221 aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc gaatatgatg
    23281 aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa cggtcgagca
    23341 gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa attagcagaa
    23401 gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa atggctcgga
    23461 gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa
    23521 cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt gacagaagca
    23581 ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt tgatgggatc
    23641 accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga gatgcgttct
    23701 ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca tcagcagatg
    23761 agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg aaacggaaca
    23821 aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa agaaataaaa
    23881 caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa tctcgatgcg
    23941 aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt tttggtttca
    24001 tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt tttccaacag
    24061 ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga tcatttagag
    24121 caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga accagcagca
    24181 gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga aaaacaaatc
    24241 acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact agaaaattcc
    24301 tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag
    24361 caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg gattgggtat
    24421 tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat aaaagttgaa
    24481 aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat gactacgcag
    24541 aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa
    24601 gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga atccttacgt
    24661 agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga attgaatgcc
    24721 gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct tcaagaaaat
    24781 ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt aaaatatcta
    24841 gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc gctcgcacag
    24901 ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca gctattgaaa
    24961 gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca aattgctcgt
    25021 cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac ggaacacccc
    25081 atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt agaacaacaa
    25141 ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa aagtgaagca
    25201 catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat ggcaacgaat
    25261 atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc atttcctgag
    25321 ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt agatagacga
    25381 aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca agcagaaaag
    25441 ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa tcagctccag
    25501 caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga tagtaactgt
    25561 ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa tcaactagct
    25621 gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg gacacagctt
    25681 gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga
    25741 atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt
    25801 gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta tcatgaagag
    25861 caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac tgagacattc
    25921 ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat cgaaataaag
    25981 caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat ctacgatgaa
    26041 tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca attacagcag
    26101 ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg gtatgtgctt
    26161 caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg tttaacgaaa
    26221 aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa aaccggatta
    26281 gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc
    26341 ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt gatccagaca
    26401 caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg gtcattagat
    26461 gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga aggaagaatg
    26521 atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca aataagaatt
    26581 gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa agggagattg
    26641 gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca atacttgcaa
    26701 gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca tgcagaagtc
    26761 ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat agattactgc
    26821 caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt ggaactttat
    26881 ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct agaaccggta
    26941 aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa aacacaaaac
    27001 aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat tgagactaat
    27061 cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc attggcatca
    27121 ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta cacagaaaaa
    27181 gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc gtttcaaaaa
    27241 gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca attattaggt
    27301 acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct cccagtagaa
    27361 caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct gcagataaat
    27421 gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc attcaaagtg
    27481 gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt tttctttatg
    27541 cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata tcaacaaacg
    27601 gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt agtgttcaag
    27661 aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga gatcggcgac
    27721 gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga atttattttc
    27781 tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta tggcgaagtc
    27841 atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca ggtgttacgt
    27901 gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta ccagaaaaat
    27961 gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt tttccgcaca
    28021 gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg
    28081 tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc
    28141 gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt cctgcaaagc
    28201 ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc ttttgattca
    28261 gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat acgtacagaa
    28321 gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca attggaaaaa
    28381 agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat tcatccagaa
    28441 agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta tcaaaagcaa
    28501 ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt agccgatgaa
    28561 atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa agaagaacgg
    28621 aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg gcaggcagag
    28681 gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa aaaagaacgt
    28741 gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag cttaagacag
    28801 gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga ggcacaaatg
    28861 gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt acctcagcgg
    28921 tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc attgtttgca
    28981 acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc cgcagaagaa
    29041 attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt gttgaaagac
    29101 ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca aaaaactgtt
    29161 tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc tgaagcattc
    29221 aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat ttgctgcgat
    29281 cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca agtaaaagat
    29341 ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca atttacaggg
    29401 atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt ttatttaaga
    29461 ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc tggcgaaaaa
    29521 gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac gggagcagat
    29581 acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc tgctggacgt
    29641 gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg
    29701 attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca aaaagtgatc
    29761 caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat gattttaagt
    29821 gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt aagaaagcct
    29881 ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg gattccattt
    29941 tatcccagct tccttgctat cattttttta tttctatttt taaaggttat acaaagattg
    30001 tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa attggagtct
    30061 gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca ctttttgcac
    30121 ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga agcatatgaa
    30181 attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct gttgcttgag
    30241 aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc agtttttttg
    30301 tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt tccttcaaga
    30361 aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct tcctcgtaaa
    30421 ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa tcttttagtg
    30481 attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc attacttttg
    30541 ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata tctgcatctg
    30601 aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg
    30661 tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt cggttttcat
    30721 tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa aagctgataa
    30781 ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt tatatttcag
    30841 tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat taatcattat
    30901 ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa agatttattt
    30961 ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg atttatttgt
    31021 ttatagtaaa ttttattta
//
LOCUS       DAZUKT010000028        30806 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000028 DAZUKT010000000
VERSION     DAZUKT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.028"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..316)
                     /locus_tag="ACG816_001273"
     CDS             complement(152..316)
                     /locus_tag="ACG816_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920157.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            611..1939
                     /locus_tag="ACG816_001274"
     CDS             611..1939
                     /locus_tag="ACG816_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4920158.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2141..2974
                     /locus_tag="ACG816_001275"
     CDS             2141..2974
                     /locus_tag="ACG816_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4920159.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2990..3727
                     /locus_tag="ACG816_001276"
     CDS             2990..3727
                     /locus_tag="ACG816_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4920160.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3882..4850)
                     /locus_tag="ACG816_001277"
     CDS             complement(3882..4850)
                     /locus_tag="ACG816_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4920161.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5082..5915
                     /locus_tag="ACG816_001278"
     CDS             5082..5915
                     /locus_tag="ACG816_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4920162.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5949..6788
                     /locus_tag="ACG816_001279"
     CDS             5949..6788
                     /locus_tag="ACG816_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4920163.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6814..7374
                     /locus_tag="ACG816_001280"
     CDS             6814..7374
                     /locus_tag="ACG816_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4920164.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7573..9294
                     /locus_tag="ACG816_001281"
     CDS             7573..9294
                     /locus_tag="ACG816_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4920165.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9459..10601
                     /locus_tag="ACG816_001282"
     CDS             9459..10601
                     /locus_tag="ACG816_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4920166.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10617..11828
                     /gene="thiI"
                     /locus_tag="ACG816_001283"
     CDS             10617..11828
                     /gene="thiI"
                     /locus_tag="ACG816_001283"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4920167.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12401..13048
                     /locus_tag="ACG816_001284"
     CDS             12401..13048
                     /locus_tag="ACG816_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4920168.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13177..13393
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13441..16086
                     /locus_tag="ACG816_001285"
     CDS             13441..16086
                     /locus_tag="ACG816_001285"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4920169.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16396..17709
                     /locus_tag="ACG816_001286"
     CDS             16396..17709
                     /locus_tag="ACG816_001286"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4920170.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17699..18352
                     /locus_tag="ACG816_001287"
     CDS             17699..18352
                     /locus_tag="ACG816_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4920171.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18407..19099
                     /gene="radC"
                     /locus_tag="ACG816_001288"
     CDS             18407..19099
                     /gene="radC"
                     /locus_tag="ACG816_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS4920172.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19249..19449
                     /locus_tag="ACG816_001289"
     CDS             19249..19449
                     /locus_tag="ACG816_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4920173.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19703..21382)
                     /locus_tag="ACG816_001290"
     CDS             complement(19703..21382)
                     /locus_tag="ACG816_001290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4920174.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21384..21596)
                     /locus_tag="ACG816_001291"
     CDS             complement(21384..21596)
                     /locus_tag="ACG816_001291"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4920175.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21989..23719
                     /gene="efrA"
                     /locus_tag="ACG816_001292"
     CDS             21989..23719
                     /gene="efrA"
                     /locus_tag="ACG816_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4920176.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23716..25476
                     /gene="efrB"
                     /locus_tag="ACG816_001293"
     CDS             23716..25476
                     /gene="efrB"
                     /locus_tag="ACG816_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4920177.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25571..25768
                     /locus_tag="ACG816_001294"
     CDS             25571..25768
                     /locus_tag="ACG816_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920178.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25921..26406
                     /locus_tag="ACG816_001295"
     CDS             25921..26406
                     /locus_tag="ACG816_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4920179.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26510..27103)
                     /gene="clpP"
                     /locus_tag="ACG816_001296"
     CDS             complement(26510..27103)
                     /gene="clpP"
                     /locus_tag="ACG816_001296"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4920180.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27283..27810
                     /locus_tag="ACG816_001297"
     CDS             27283..27810
                     /locus_tag="ACG816_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920181.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27901..28638
                     /locus_tag="ACG816_001298"
     CDS             27901..28638
                     /locus_tag="ACG816_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4920182.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28642..29559
                     /gene="miaA"
                     /locus_tag="ACG816_001299"
     CDS             28642..29559
                     /gene="miaA"
                     /locus_tag="ACG816_001299"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4920183.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29561..30790
                     /gene="hflX"
                     /locus_tag="ACG816_001300"
     CDS             29561..30790
                     /gene="hflX"
                     /locus_tag="ACG816_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4920184.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10174 a         5271 c         6572 g         8789 t
ORIGIN      
        1 tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt
       61 ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc
      121 tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata
      181 cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga
      241 cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag
      301 caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa
      361 tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac
      421 aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt
      481 atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata
      541 gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa
      601 ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca
      661 agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc
      721 tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac
      781 ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac
      841 attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta
      901 cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac
      961 aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga
     1021 tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc
     1081 ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa
     1141 attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga
     1201 cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc
     1261 attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca
     1321 tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga
     1381 tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac
     1441 agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat
     1501 ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg
     1561 ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc
     1621 aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg
     1681 agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc
     1741 aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt
     1801 tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt
     1861 gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc
     1921 aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga
     1981 gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg
     2041 tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg
     2101 atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt
     2161 atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac
     2221 ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa
     2281 aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc
     2341 ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga
     2401 gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac
     2461 agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc
     2521 ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt
     2581 ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga
     2641 acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct
     2701 gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt
     2761 ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc
     2821 ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact
     2881 gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg
     2941 ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa
     3001 atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt
     3061 tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag
     3121 aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga
     3181 gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta
     3241 caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt
     3301 caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa
     3361 tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa
     3421 aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga
     3481 tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata
     3541 tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga
     3601 gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac
     3661 aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa
     3721 taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg
     3781 aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt
     3841 cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa
     3901 atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt
     3961 tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct
     4021 tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg
     4081 attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca
     4141 cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc
     4201 ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc
     4261 agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg
     4321 atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg
     4381 tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct
     4441 tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca
     4501 ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca
     4561 agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata
     4621 cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg
     4681 tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg
     4741 atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt
     4801 tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact
     4861 tccttacgaa taattttgca tagccgttaa atcttattgt atactatata aaagagtttt
     4921 gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa
     4981 tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac
     5041 aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa
     5101 catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg
     5161 cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct
     5221 cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc
     5281 aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg
     5341 cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag
     5401 tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta
     5461 gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg
     5521 atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca
     5581 tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga
     5641 actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag
     5701 agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac
     5761 atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt
     5821 ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag
     5881 atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg
     5941 cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt
     6001 caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat
     6061 gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat
     6121 tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg
     6181 accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc
     6241 gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac
     6301 aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga
     6361 tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag
     6421 agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa
     6481 agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata
     6541 tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa
     6601 ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag
     6661 gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact
     6721 ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga
     6781 tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa
     6841 gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca
     6901 gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca
     6961 gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta
     7021 gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa
     7081 caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc attactagga
     7141 atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca
     7201 gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag
     7261 atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg
     7321 attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag
     7381 tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt
     7441 gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat
     7501 tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg
     7561 gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc
     7621 gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat
     7681 gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat
     7741 gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa
     7801 tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa
     7861 aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct
     7921 acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg
     7981 gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg
     8041 aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa
     8101 cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac
     8161 cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct
     8221 ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt
     8281 gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa
     8341 gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt
     8401 gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat
     8461 tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa
     8521 tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt attgattgag
     8581 ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt
     8641 ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt
     8701 caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta
     8761 gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc
     8821 gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt
     8881 tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt
     8941 gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa
     9001 gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca
     9061 catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat
     9121 cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa
     9181 gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct
     9241 ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa
     9301 aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta
     9361 tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg
     9421 ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg
     9481 caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta
     9541 ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag
     9601 caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag
     9661 gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg
     9721 ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat
     9781 taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg
     9841 tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga
     9901 ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc
     9961 ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat
    10021 ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg
    10081 gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag
    10141 ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta
    10201 aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa
    10261 aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa
    10321 ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg
    10381 tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta
    10441 aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg
    10501 ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta
    10561 ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga
    10621 aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa
    10681 gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca
    10741 aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag
    10801 ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag
    10861 aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg
    10921 ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta
    10981 atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc
    11041 aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat
    11101 cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc
    11161 ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag
    11221 tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa
    11281 aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag
    11341 tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga
    11401 caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt
    11461 tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg
    11521 tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac
    11581 ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg
    11641 aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg
    11701 tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct
    11761 tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat
    11821 tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa
    11881 aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc
    11941 atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta
    12001 tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc
    12061 gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt
    12121 tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta
    12181 tcagaggttg cgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagt
    12241 tcctcatatt ttacgtttgg ttgagcttat taccgcagat gcaagctttt gaacaccgtt
    12301 ttatttgtct cacacccttt tccttcctac ttgtaacctt gctcccgtgt tagaaacatg
    12361 ttacactaaa tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa aaacagatac
    12421 actgccaaaa gcaactgcga aaagattacc tttatattta cgctacttga agatgctgga
    12481 tgactcaggt ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc aaattccttc
    12541 tgcaacgatc cgcagagact tctcgcaatt aggcgagcta ggcagaagcg gatatggcta
    12601 tgatgtccct tttcttatcg atgtattcaa taatatcctt aatactaaag aagaaaaacg
    12661 aatcgcatta gtaggatatg gaaatcttgg caaagcattg aaacacaaca attttagaag
    12721 aaatgaaaat ctaaatatcg tgtgcgtctt tgataatgat ccagcactga tcaatcgagt
    12781 aattgatgga gaaatgatct atccgattga tcgtttcgcg gagatagcca aagcaaaaaa
    12841 tgtgacagta gcaatctcta cggtaccaag taaatattca caatctgcta ttgatgaaat
    12901 tgtcaaagga aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa ctgtcccagc
    12961 ttatgtcaat gttcaataca ttgatttgac aaccgagttg cagacgctta tttactttga
    13021 tgagaattac tcagaagttt tttcttagga aaatttgagt aggcttattg atcttgcgag
    13081 aactcgtcgt aattctttca aaaatttttt agtactaaat gaagtggttt tttcacgacc
    13141 acttgttgac taaaaaaaaa aaccattgta gaatgaagca taacgctgaa caagaggagt
    13201 aactggcaca ttcattctta gagagagaac tatctgctga aaggttctca atgacaacca
    13261 gtgaaggtag cttgggagtt tctgtcttga atggagtaga gatagacgag tcgtgatcgt
    13321 taactcactt gagaggcaat aatgattgat tattgcaatt taggtggtac cgcgtaaatc
    13381 atacgtccta gaatacgaaa gtattttagg acgtttttta gtttaaagga ggaaaacagc
    13441 atgacggaag aaaataacct atcaacaaaa tataatccgc aagaagttga agctggacgt
    13501 tatcagcatt ggttagaaca agatttgttt aaaccgagcg gagataaaaa agcaaaacct
    13561 tattcaatcg taatcccgcc cccaaatgtt actggaaaac ttcatttagg gcatgcttgg
    13621 gatacgacct tgcaagatat gctgattcgt caaaagagaa tgcaaggata tgatacttta
    13681 tggctgcctg gtatggacca tgcaggaatc gcgacacaag caaaagtaga agcgaagctt
    13741 agagagcaag ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga tcaagtgtgg
    13801 gaatggaaag aagagtatgc gagccacatc cgcgaacagt gggctaaaat gggcttatct
    13861 ttagattaca gtagggaacg ttttacatta gatgatggat tatctgaagc agtacgtaaa
    13921 gtttttgtta ccttatacga aaaaggtttg atttatcgtg gtgaatatat catcaactgg
    13981 gatcctcaag cacgaacagc tctatctgat atcgaagtta ttcataaaga tatcgaaggc
    14041 gccttttatc atatgagcta tgaactagca gatggatcag gcgtagtaga aattgcgaca
    14101 acccgtccag aaacgatgtt gggagatacg gcgattgctg ttcatccaga agacgagcgt
    14161 tatcaagcgc tcatcggcaa aaaagtgatt ctacctttag tcaataaaca aatcccaatc
    14221 attgcagatg aatatgtaga tatggaattc gggacaggtg tagtaaaaat cactccagca
    14281 catgatccaa acgactttga ggtaggtaat cgtcatgatc ttccacgcgt aaatgttatg
    14341 gatgatgatg gtacgatgaa tgaactagca ggaaaatatg ctggaatgga acgttttgct
    14401 gctcgtaaag caatcgtgca agatctgaaa gaaatcggtc gattgattaa aatcgatacg
    14461 atgatccaca gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc tcggctgtct
    14521 actcaatggt ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa ccaagaaaca
    14581 gatcaagcag tggaattctt ccctccaaga ttcaatcaaa cgtttttacg ttggatggaa
    14641 aatgtccatg attgggtaat ctcacgacaa ttatggtggg gacatcaaat tcctgcatgg
    14701 tatcacaaag aaacaggtga aatgtatgta gggatggaag caccagcgga tagtgaaaac
    14761 tgggtacaag atcctgatgt tttagacact tggtttagtt cagctttgtg gcctttctca
    14821 acaatgggct ggccagatac ggaaagcgaa gattataagc gatacttccc tactagcact
    14881 ttagtaaccg gttatgacat cattttcttc tgggtaagca gaatgatttt ccaaagcttg
    14941 gaatttactg gcgaacgtcc atttgagaat gtcttgattc atggattgat tcgtgacgaa
    15001 caagggcgga agatgagtaa atcattaggt aatggaatcg atccgatgga agtaatcgaa
    15061 aagtatggag cagatgctct ccgctggttc ttatcgaatg gttctgcacc aggacaagat
    15121 gtccgcttca gctatgaaaa aatggatgct tcttggaact ttatcaacaa aatctggaat
    15181 gcttcacgtt tcgttatcat gaatatcgaa ggaatgaccg tagaagagat cgatttatct
    15241 ggagaaaaat cagtggctga ccggtggatc cttactcgtt tgaatgaaac gatcgaacga
    15301 gtaactgagc tatttgatcg ttttgaattt ggtgaagcag gtcgacaact gtataacttt
    15361 atctgggatg atttttgtga ctggtacatc gaaatgagta aagaagtact ttatggagat
    15421 gatgctgttt caaaacaaac cacccgtagc atcttagtcc atacacttga tcagatccta
    15481 cgtttattgc atccaatcat gccattcgtt acagaagaaa tatgggaaca tattcctcat
    15541 cagggaaatt ctctagtagt ggcagaatat cctgttgtac gtccagagtt tgatgatgaa
    15601 acagcttcta aagggatgga agtattgaaa gaattgattc gagccgttcg aaatattcgt
    15661 tcagaagtga atacaccatt gtctaaacca attacgttgc taattaaaac aaacgatgct
    15721 aaaatcgatc aatttttagt tgaaaataca agctatatcg aacgcttctg taatcccgaa
    15781 gagttgacga tatcaagtga gatcactgcg ccagatctag cgatgtcagc tgttctgaca
    15841 ggtgcagaaa tcttcttgcc gttagcagga ttgatcaata ttgaagaaga aatcaaacga
    15901 ttagaaaaag aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg aaaactttct
    15961 aatgaacgtt ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg tgcaaaagaa
    16021 aaagattatt tagagaagca aacggctgtc aaagaacgaa tcgaaagttt gcgtactatt
    16081 caataagaat agaaaaagag agtgtgaaat cagctagcta ttactaaaag ctagctgatt
    16141 gaacatcgtt acaggaaaga gtttgagcct cactcttagc ttcgattcat aaggagatgt
    16201 gacataaatt ttgtcgcatc tcttttttag tctactctta ttatgtagga gaaggaggga
    16261 ggtggatcat gtatcaagag tcaataaagt agttgataaa atcgttcctc tctctgtttc
    16321 ttttctcttt gtcatacttt tatttttctt atgtgttcta tttgttataa taaaagagaa
    16381 atgggggaat tgttgatgac gattgaagaa gcaatagaat ggatccatag tcgattacca
    16441 tttggttcac gtcctggcct tgaccgagtc gaagctttat tagaagtggt agggcatcca
    16501 gagagaaaag taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc tactgtagct
    16561 tatttgagaa gcctattaga agaggctggg ctgacagtag gtacatttac ttcaccttat
    16621 atcgaaacgt tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga caatgatttg
    16681 atccagcttg taaaaaaata tcagccactg atcaaacaac ttgatcaatt tgaagcagta
    16741 gctggtatta ctgaattcga gacattaaca gcaatggcat ttgattattt tttgatgaag
    16801 aaagtggacg tagcgattat cgaagttggc ttaggcggct tattggattc taccaatgta
    16861 gctgtaccaa tgctgacggg aataacaacg attggacttg atcataccga aatacttgga
    16921 gatacaattg aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg cgttccagta
    16981 gtaacaggaa atattgcccc agaagctttt actgtcatcc aacaaacagc taaagcaaaa
    17041 aatgcaccgc tttatgtata cggggaaaat tataaaataa actatctgca tcctgatgag
    17101 atgtggggag aagtatttga attttttaat gaaataggaa agatccctca attaaccact
    17161 ccgcttttag ggcaacatca agctgaaaat gcaggagtag ccatcgaatt gtattttgtt
    17221 tactgtcaat tggaaaagct gcctttccga gaaaaagatg ttttgaaggg attgaagaaa
    17281 tctcgatggc caggaagaat ggagcgtttg agtaacgagc cattagttgt tttagatggt
    17341 gcccataata cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga attcaaagga
    17401 tatcaaatca acattttgtt ttccgcctta cgaactaaaa atgttagaga aatgctagaa
    17461 cgattgcagc aagtaccaaa tgtccatatt tatttgacga cctttgaaca tccaaaagcc
    17521 atcgaattaa gtgaagtatc agatttagca agtgagagag tatcaatcgt ttcattgtgg
    17581 caattcggat tagcagatat attggaaaag atgacaaatg aagacgtatt agttgtgacc
    17641 ggttcacttt atttcatttc agaagttcgc catttattaa tacaattagg aggagatcat
    17701 gaagtataat gcagtgattt ttgacatgga tggtttgctg ttcgacacag agttagtgta
    17761 ttacgaagcc acacaagtag tagcagacaa gatgggtttt ccttatgata aagacctgta
    17821 tttaaaattc ttaggtgttt cagatgaaga agtatgggcg aattatcata tgattttctc
    17881 ggaatttggc aaaacaagtg tacaaagatt catagatgat tcataccagg aaacgcttga
    17941 acgtttttcg caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc tctctttttt
    18001 agaagaacat cagattccca aagttgtcgc ttccagtaac caacgagcag tcattgaact
    18061 tttattgaag aagaacaatc tgtttgaacg atttgagacg attgtttctg cagaaaatgt
    18121 tcaacgagca aaaccagacc cagaaatttt cttgttagct aatcagaagc taggtacagc
    18181 gaagaaagaa acattgattt tagaagattc acaaaatggt attttagcag cttctgcggc
    18241 tgagatcccg gtagtcatgg taccagatct gcttccacca tcccaagaat tgatcaaaaa
    18301 aacagctggg gttgtttctt ctttacatga aatccctgca ttgttaaaat aaaaatccat
    18361 ctcttttcgt atcttatttt agataagata gaaaggagat gtttttttgg gaaaacgatt
    18421 gatcaaagaa gtacctacta gctctttacc aagggaacgt atggaaattt atggcgcgga
    18481 agctttatcg gatcaggagc ttttggcaat cttactgcgt accggtcaac acccttatag
    18541 tgtgatgtcg attgctggaa atctgttaaa aacgtttgga ggactggcct ctttacgaca
    18601 ggctactttg catgagttag aagagattcg agggatagga cgcgtgaaag caatcgaaat
    18661 caaagccttg atcgaattag gtcgacgaat tcagacggat catgaaagag cggctccacc
    18721 ggttcgtacc agctacgaat tggcccagca attgattctg gaaatgaaag atcacaaaca
    18781 agaacatttg gtttgtattt atttggacac gaagaatcaa gtgatcttaa aaaaaacagt
    18841 gttcatcggt tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc attatgctgt
    18901 acgttattgt gcagcaagga tcgttttagc gcataatcat ccttctggta atgttattcc
    18961 atcgcagcag gatatgaatt ttaccaaaag gatacaaaag tgtggagaaa tgatggggat
    19021 cactgtactg gaccacttga tcatcggacg caaacgatat tttagtttga gagaagaagg
    19081 aatgatggaa gagaagtaag ttgctttctt gcaaaaaaaa tgaagcaagt gtacaattat
    19141 attgtaattt tgtttgggaa tagaagttga aacatctgtt gcacctctat cgtacacgag
    19201 caatgttaca atttaaatta gtgcttttgg cacgaggagg atattattat ggaaaacgga
    19261 acagtaaaat ggttcaacgc tgaaaaagga tttggattta tctcacgtga agatggaagc
    19321 gacgtattcg ttcacttctc agctatccaa ggtgacggat tcaaaacatt agaagaaggt
    19381 caagcagtga ctttcgacgt tgaagaatca gaccgcggcc cacaagctgc aaacgttgta
    19441 aaagcttaat tttatctgta aatcaaattg atggtagaca tcaattttta cgagagaaca
    19501 aagcgagtaa caaaaatacc tgccacaaga gacaatttct cttgtggcag gtattttttt
    19561 gtatgaaaaa gtaaataaat ttaagaggct atgaaagagc caccttgtat caaggaataa
    19621 gaacaaacaa aaatggcttc tcatgttttt catttgtttt ctcttatatt caggacaagg
    19681 tgctgatttc ataacctctt atttattctt catctggtgt cattaccatt ggcaaaatca
    19741 ttggatgtct ttctgtacgt tcgaataaga aaggctgaag cgcgccagtc atcgcttctt
    19801 tcaatttata ttctgaacag tcaggtccat tcaaacattc tcttaaagcg tggaataaga
    19861 ttcgctgagc ttcattgatc atttctcctg attctcgcat gtagacaaat ccacgagaga
    19921 gaatatctgg accagccaag atttcttttt tcttgatatc cactgtcgcg acagctaata
    19981 caagaccttc ttctgaaagc aggtgacggt cacgtaaaac gacattacca atatcaccga
    20041 tcccgctacc atcgatatac acatctccag ccacaaaatg cccagcagga cgcgcagaat
    20101 cgctagttaa cgctaaaaca tcgccatttt ccatgatgaa gcagttttct gcaggaacac
    20161 ctacatcttg tgctagagaa gcatgtactt tcaacatgcg gaattctccg tggacaggaa
    20221 taaaatattt tggcttcatc aaacgaagca ttaatttttg ttcttcttgt ccaccgtgac
    20281 cggaagtatg gatgttattg atttttccgt gaatcacttc tgcaccagct tcagacagca
    20341 aattgatcaa atgattgaca cttgttgtat taccaggaat aggtgaactt gaaaagatca
    20401 ctgtatcgtc tggctggatt tggatttgac ggtgagtacc attcgcgatg cggctcaacg
    20461 cggccatcgc ttctccttga gaacctgtac ataggatcat cgtttcattg gcaggcaagc
    20521 gattgatttc gtgtgcatct acaaatgtgc cgtctggaac tttgatatat cctaaacgtt
    20581 gaccgttgac gatggcattt tccatgcttc gtccgaaaac agcgatttta cgaccagttg
    20641 ccacagcggc atctgctgct tgctgcaaac ggaagatgtt agaagcaaag ctggcaaaaa
    20701 tgatacgtcc ttcgattttt gaaaagatct tcaaaataga atgcccaatc gttttttctg
    20761 atttagtaaa tgttggaatc tcagcatttg tactatcaga taaaaggcac aagactcctt
    20821 cttccccgat acgagccatt ttatgcaagt ttgctggttc gccaacagga gtgaaatcga
    20881 atttgaagtc tccagtcgct acgatatttc cagaaggtgt tttcaccacg atccctaatg
    20941 catctgggat actatgggtc gttctgaaaa agctgacaga agtctttcta aaacggatga
    21001 tcgtatcttc attgatctca tggagctctg catctcgaag caagccatgc tcatctaatt
    21061 tgtttgtgat caacgctaat gcgagaggac cagcatagat aggaatattt gcttgtttca
    21121 gcaagtaggg tattccgccg atgtggtctt cgtgcccgtg agtaatcacc aaggctttta
    21181 ctttgtgtaa gttttgtatg atgtagctgt agtcgggaat gacatagtca attccaagta
    21241 aatcgtcttc cgggaatttg atcccggcat caatcaaaat gatttcatct tggaattgta
    21301 ccccatacgt atttttcccg atttcaccta atccaccaat agcaaagacg cctgtctcgt
    21361 tatttttgat ggaaagtttc atattaaaac tccactaatg agaaatcagc attttctttt
    21421 tcgtactcta gatgacttcc ttctagaagt tgaacgaatt caatattgta tggggtattt
    21481 tcttccacac gttggcgagc gtctacatcg ctttttgcct caatgtaaag agattttgtt
    21541 tcttcccgtt tagggttgcg tacttttgtt tcttgataat aaactttata gatcattgtt
    21601 ttgtctcctt tatctgcgta aagcagtgat ttagctagtt gattttttta gacaacacaa
    21661 aacgagggga caagccacct ggtttttcat atgctttgac tagaatactg gattcgagtc
    21721 aaagagacaa gcctcgtttt tgcttatctg ttcggatatt ttttcaaccg taccttctgt
    21781 ataaaaaata tacaacaatt aattgttatt ctagcataaa acagcaaata agtatagaaa
    21841 tcaactagat tgaaagaaaa aatcaatgtt tatcatcccg ctataatcat aatgactttt
    21901 ttatgagaga agtgttctat taataggctc gttgtgctat catttttagt aatgaaaatg
    21961 taacaaaaga ttaaaaggag atgagaaagt gaaactaatg tggcattata ccatgcgtta
    22021 taagaaattc ttgtttttca atttcatatg cgtatttggt tttattttga ttgaattagg
    22081 acttcctacg attttagcac gtatgatcga tgtaggaatc cgcaatgatg attttgatta
    22141 tgtgaagcaa caagggatat ttatgatcgt tattactgta atcggtatta ttttgaatat
    22201 cttgttaggt tattttggtt cgaggattac gactaatatc gtagcagata ttcgagatga
    22261 tttgttcgaa aagatccaaa gttattcgca tcaggaatac gaaaatctag gcgtatcttc
    22321 attgatcact cgtacaacaa acgatgctta tcagatcatg ttgttcctgc aaaatatttt
    22381 acgtatcggt tttatggcac caatgatgtt cattgtcagt ttgtacatgg tcatgcgtac
    22441 aagtgtgaca ctcagtttgt atgtagtagg tgcattacct ttactattat tggcagtcgt
    22501 agcgattgct aaggtgtctg aaccgttatc taaaaagcaa caaaaaaact tagataaaat
    22561 caacagtatc ttgcgggaaa atctttcagg attgcgtgtg attcgtgcat tcgtcaatga
    22621 aaagtttgaa gaaaaacgtt ttgctgtagt caatgagaat tatgcttcta gttcaaaaag
    22681 cttattccgt ttgatggctg ttgcacaacc gggtttttat ttcttattta atatcgtaat
    22741 ggtattgatc atctggagcg gggcagtgca aatcgatgca ggcgagcttc gtgtaggaga
    22801 tttgattgcg tttatcgagt atatattcca tgccttgttc tcattcatgt tatttgctag
    22861 tgtgttcatg atgtatccaa gagcagctgt ttcagctgga cgtatccaag aagccctaga
    22921 tgccaaacct tcgattgtag aagatccaaa tggtatatct gatacaaaaa ctaaagggta
    22981 tatcgaattt caaaatgtaa catttgctta tccaggccat tcgcaagagc cagtgatacg
    23041 aaatgtcagt tttacagcct ctccaggaga gaccgttgct tttattggaa gtaccggtag
    23101 cggaaaatct acgttgatcc aattgatccc tcgcttttat gatgtgagtg aaggaaagat
    23161 cttgatcgat ggagtagacg tgagagatta tcggctaagc aagttgcgag ataagattgg
    23221 gtttattccg caaaaagcgc tgctatttac tggaacgatt gcagataatt tgcgatacgg
    23281 gaaagaagat gctacgcaag aagaaatgga acgtgcagct gagattgctc aagcttcaga
    23341 attcatttct cgaaaaccag atggatttaa tgaacattta tcagaaggtg gttccaattt
    23401 ttcaggtgga caaaaacaac gattggcaat agccagagcc atcatacgcc gtccggaaat
    23461 ctatattttt gatgacagct tttctgcatt agattatcaa acggatgcga agttacgtgc
    23521 tcgtctgaaa aaagaaacga cagaatctac tgtattgatc gtagcacaac gtgtgggaac
    23581 gatcatgcat gcagataaga tcatcgtatt aaatgaagga gaagtcgtcg gtatggggac
    23641 gcatcgggag ttgttggaaa aatgcccgat ttattatgat attgccgctt ctcaattatc
    23701 aaaggaggaa ttagcatgaa aaaaaccttt tcttccttaa aacgtttagc tcgatatatc
    23761 agaccgtatc gtgagacttt tcttttagtg atcctattta cagttctgac agtggctttc
    23821 aacgtggctt tgccatatgt tacaggactt cctactacag aaatcagccg taatattgcc
    23881 aatggcgaat caatcaattt ttcttatata ttcaaatgtc tcatttggat cacagttgtt
    23941 ggaatagggt attgtatttc gcaattactt tctggtgttt tgatgacaaa tgtcgttcaa
    24001 agtgccatgc atgatttgag aagagacata gaagaaaaaa tcaatcgtct tccggtttca
    24061 tatttcgata aaaaccagca aggaaatatt ttgtctcgtg tgacgaatga tgtagatgca
    24121 gtcagcggtg ctttacaaca agcttttatt ggtatcgtaa atgcgatatt gggaattacg
    24181 ttggctgcag caatgatgtt ttatatccag ccgatgatgg cgctgatctc tatgatcatg
    24241 attccaacat cgatctggat ctcaaaacga gtgatcaatg cttcgcagaa atattttcaa
    24301 agcatgcaga attctcttgg tgagctaaac ggctacgtcc aagaaaatat gacaggtttt
    24361 agtgtattga aagtatatgg acgcgaaaaa gaaacattgg aaggattcaa aaaagttaat
    24421 cacagcttga aatactttgg gtttcgtgca gcattcattt ctggcttgat gatgcctcta
    24481 gttcaattaa ctgcctatgc tacttatatt ggtatggctg ttctaggaag cttttatgtt
    24541 attactgggg cgatcgttgt cggtcaacta caagcattca tccaatatat ttggcaaatc
    24601 agtcagccaa tggggaacgt gacacaattg tcttcagttt tgcaaagtgc atcagcggca
    24661 acgaaacgtg tattcgagat tttagatgag ccagaagaaa aagaaaacaa agtagatgct
    24721 tcactgccag aaacgatttt aggagatgtg acattcgatc atgtcgattt tgcatatgat
    24781 ccgaagaaac cattaatcca agatttgaat tttgaagtga aagctggtca aaccgtagct
    24841 gtggtcggtc cgaccggtgc cggcaagacg acattgatca atctattgat gcgtttttat
    24901 gacgtagaca aaggagcaat aaggattgat ggaatcgaca ccaaatcaat gagtcgcagt
    24961 gatgtgcgtt cgctatttgg tatggtcttg caagatgcat ggctttatca aggaacgatt
    25021 gcagataata tccgcttcgg aaagcttgat gcgacagatt atgaagtagt tgatgctgcg
    25081 aaaacagcaa acgtagatca ttttatccga acgatgccag atggatatga tatggccatc
    25141 aattctgagg gagacaatgt gtctttagga caaaaacagt tgctgacgat tgcccgagca
    25201 gtggtctccg atcctaaaat cttgatttta gatgaagcaa caagttcagt agacacccgt
    25261 ttagaagcgt tgattcaaaa agcaatggat cgcgtgatgg agggacggac aagtttcgtc
    25321 atcgctcacc gtttatcgac aatccgtgat gcagatctga ttctagtgat ggatcaaggt
    25381 cagatcattg aaaaaggaac gcatgagagt ttattggcac aaggtggatt ttatgaaaaa
    25441 ctgtataaca gccaatttgc tgaggaagca gaataagtca tataagaata aaacagttta
    25501 aaacttattt tttgttctct attcttgata atggtaaagt aaagttatca agaaaagtga
    25561 ggacacggag atggaacagt ttttaaatga aaaaattact gaagccaaaa gagaagttga
    25621 tcgtttaacg cacgtctgtc agacgaagcc ttttgattca gctgattttt ttgaaaatga
    25681 gtttcaaatc gaacgcttgg cagcacaaat agaagtgtac gaattaattt taaaaaaagt
    25741 aatagaagaa aatatagatg tacaataaaa acaggtaaaa cgtcagttac cagatactgt
    25801 aaatagacat ctggcctgag caggttttac ctgttttttt tcttatgaaa taaatatatg
    25861 ctatgatacc tttattcaat aggattagtt tctacaaaaa atgaaaggag aaaaataaat
    25921 atggaacggt ttatagcatc aaatgccaca gtgattggtg atgtgacatt gtcagaagat
    25981 gtgacgatct ggtatcaagc tgttttacgc ggggacagta attggataaa aataggacaa
    26041 agaacgaata tccaagatgg tacaatcatc catgtcgatc atgatgcacc tgtagatatt
    26101 gcggagaatg tgacggtagg acatcagtgc atgctacatg gctgtacaat cgaaaaaggg
    26161 gcgctgatcg ggatggggac cacgatattg aatcatgcgg ttattggtga gaatagtttg
    26221 attggtgctg gatcattagt aacagaagga aaagttattc ctcctaatgt cttggctttt
    26281 ggtcgcccag ctaaagtgat ccgtcctttg acaaaagaag aaatacaaaa aaacaaagaa
    26341 aacatccagc attatgtcga gataggtcgg atgcatgcgc aaggaaaatt caaggagcaa
    26401 agataggcag tagcatccat aaaataaaaa aggatggtca gtttgagttg accatccttt
    26461 tttatttggt ttagataaga gagcttaaaa aagcttcctt tgaaacagat taatttaatg
    26521 aagaactgtt ttccatgact tcatcgatca atccatatgc ttttgcttgt tcagctgtca
    26581 tatagttgtc acgatctgta tctttttcaa tcacttcaag cggttgtcca gtgcgttcag
    26641 ctaaaatttt gtttaagcgt tcgcgtgttt gcaaaatatg gcgtgccgca atttcgattt
    26701 cagttgcttg cccttgagcg ccgccaagag gttgatggat catgatctca gcatttggta
    26761 aagcaaatcg tttacctttt gtaccagctg tcagcaagaa gcttcccatt gaagcagcca
    26821 tacccataac aattgtttgg acatctgctt tgacaaagtt cattgtgtcg taaatagcca
    26881 tacctgctgt aactgaacca ccaggagaat tgatataaag atagatatct ttttcagaat
    26941 cttgagcgtc taagaaaagc aattgggcga tgatcgaatt agcaagatca tctgttactt
    27001 ggccgcttaa catgatgata cggtctttca ataaacgtga gtagatatcg taagctcttt
    27061 cacctcgtga agattgttca atgactgtag gaattaaatt cataatagag ttcctccttg
    27121 atttcgttta gaaatagtgt accataattt agtttctaat acctaaaatg tagtatacat
    27181 ttttggtcaa aaaaggtcaa ataaaaaact taggcaaatt agtcgcataa acccgcttta
    27241 tggtatgata agcagagaaa caaaacaagg aggaacaaca gattgatagt tggtattact
    27301 ggccttatcg gagtgctgac aggtgctgta gttgtctata tcggcatgat actgcaaatg
    27361 cgtcacttgg aaaataaaga acagcgtaga cgtgaattgg aattgaaagt caaagaaatc
    27421 gagacgctga atcaattaaa taaaaagatc aacgagatct tgcaaaaacg gacaacgctg
    27481 atggaagaat atgtcagctt tgatgcattt gatgattgtt atatcacgat tgacgatttt
    27541 gcgtatctcc aatcattttc agcgcaaaat agtttttatc tgccaaatta tattttagac
    27601 gagctattta agaatatctc ccatcgaaaa gtgattttat ctccagatga aacaatgaag
    27661 attggtggat atgcttataa aggcggacgc gtgattttag agaattttac ggatagtctg
    27721 actgaaatga tcaatgaaaa aaaagctcag ttgaagacta tgacgcgcca gccgattggt
    27781 ttcttctcga aagatgatgt ttggagctga tattttgaag cattgccgtt gcacatgaga
    27841 gcgtggtaat gctttctttt tttcttaatg atacaataga actgaacgga gtgaagaaag
    27901 atgttacaaa tctatgccca tcgcggaagc agcggcaccc acccagaaaa tacgcttcct
    27961 gcatttgcag aagctgtacg agtaggtgca gatggtatcg aactagatgt ccatctaagc
    28021 aaagatggtt atttgatcgt tatgcacgac gaagaagtgg accggacgac taacggtaaa
    28081 gggctgattc gagaaaaaac attggaagaa ctcaaaaaat tgaatgcagg gggcaaattc
    28141 agcaaagaat ttccagcagc taaagttcct gaattgagag aagtgttagg actgctcctc
    28201 caaaaaaatt accgagggat gctaatgatt gaaattaaaa ctgatcagta tgaatatccc
    28261 ggtatcgaag aaaaattagc cgaaacatta aaaagtagag attggccttt tcaacatgca
    28321 tattgcagct ttcatttgga ttctttggaa aaaatcgcat tactggaacc ggagatacag
    28381 ctggacttat tgatgagtac ttctgataaa aagatccaac tagcagcaga aaccccttat
    28441 atagaaggag tacatccaaa aaatacttgg ttgttcgaac atccagtcca ggcagctgat
    28501 tttccaaaag caattcgtgc atggacgatt aacgatgaag aagaaatggc caaatgtcaa
    28561 gaactcaaaa tagatggaat aataactgat tttccggaga aagctcttcg gtggaaacaa
    28621 tcaacgaaaa gtaggtaaaa tatggaaaaa gtattagtga tcgtcggtcc gacagcagtc
    28681 ggtaaaacag cgctaagtgt ggaactcgca caactatttg gaggagagat catcagtggt
    28741 gattcattgc aaatctataa aaatctggat atcggtacag caaaaatcaa agaagaagag
    28801 atgaataatg ttccgcatca tttgattgat gtgatcgaac ctactcaacg ttactcagcc
    28861 gctgattttc agcattcagg tcgacaattg attaccgaga ttgcgaaccg agggcaccta
    28921 cctatcatag ctggtggtac gggtctctat atccaatccc ttctatatga ttatcagtta
    28981 ggagcgacag aagaagcgaa tcctgagatt agaaaaaagt atgaaacgtt agctgaaaca
    29041 attggttccc aagcattatg gaatcagttg aaagaaaaag acccggcagc tgctgagaaa
    29101 atccactgga acaatcaacg caaagtgata agagctctgg aagttttaga gacgacagga
    29161 catagtatca cggcacctaa agaagagcca aagcaattgt atgattacct catgattgga
    29221 ttagatacga agcgtagtat tctttatgaa cgaatcaatc aaagagtcga tttgatgcta
    29281 gcagaaggtt tagtagcaga agcgcgtaag gtgtatgagc ttggagaagt ccaggccagt
    29341 caaggtattg gatataaaga atttttccct tattttgaag ggaaagaaac attggaagaa
    29401 gcaacagaac aagtcaaact acattcgaga agatacgcga aacgtcagtt gacgtggttt
    29461 cgtaaccgtt tggatgcaca gtggtttgat cttataagtg agccaaatca aaaaagtcag
    29521 attgagcaaa caatcaaaaa atggctggag gcggaataaa atggaagaaa aagtgatcat
    29581 cgtaggggta gaaaccgaag caaaccagcg ttacttttca gagtcaatgg aagagctagt
    29641 tcagctgacg aataccgcat caggagaagt cgtattcaca attacacaaa agagaccaca
    29701 agtggatcgg caaacgatta tcggtaaagg gaaattacaa gaattagttc aacaagcgga
    29761 tgcgcatgaa gcggatttga tcatctttaa tcatgagcta actccgagac agagccaact
    29821 gattaccgat gctgttggtc ttccggtaat tgatcgcgtg cagcttatct tagatatttt
    29881 tgcgatgcgt gctcgttcga aagaagggaa gctgcaggtt gaacttgctc agttagaata
    29941 cctgctgcct cgtttagtag ggcaagggaa aacattatct cgtcttggcg gtgggatcgg
    30001 aacaagaggg ccaggtgaaa caaagttaga aacagaccgc cgacatatac ggaataaaat
    30061 ttttgctgtt aagaaagaat tgaaagaagt agaagctcat cgagagcgca atcgccaaaa
    30121 aagaaaaaat agcgagattt ttcagattgg acttattggc tacacgaatg ccgggaagtc
    30181 aactatcttg aatctattga cacaagcgga tacttattcg aaagatcaat tgtttgcaac
    30241 gttagatcca ttgactaaga gatggcgttt tgctgaagga tttgagatta ctgtaacgga
    30301 taccgtcggg ttcatccaag acctgcctac gcaactgatc gatgctttcc attctacatt
    30361 agaagaaagt caaaatatgg atcttttgct acatgtagtc gatgctagtt ctccagatcg
    30421 tattttacag gagcagactg tcttaaaatt gatggatgaa ctgaatatga aagaaatgcc
    30481 gatactgact gtttacaata aggcagacca gatcgaccca gcgatgttca cgcctacgct
    30541 attcccaaat gtattgattt ctactcagac gaaagaaggt aagatggcgt tgatcgaagc
    30601 aatcaaacgt cagctgatgg aactaatgac cccttataca ctttctgtgc caagcaacga
    30661 aggacagaaa ttaagcgagt tgagaagaca aacaatggtt ttggacgaag cgtacatgga
    30721 agaaacaaac gaatatgaag ttaaagggtt tgcaaaaacc aattcaaaat ggttaagaaa
    30781 aaaccaataa aattttattt ttttaa
//
LOCUS       DAZUKT010000029        30688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000029 DAZUKT010000000
VERSION     DAZUKT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.029"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2154)
                     /gene="glyS"
                     /locus_tag="ACG816_001301"
     CDS             complement(79..2154)
                     /gene="glyS"
                     /locus_tag="ACG816_001301"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4920185.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ACG816_001302"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ACG816_001302"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4920186.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ACG816_001303"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ACG816_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4920187.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="ACG816_001304"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="ACG816_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4920188.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="ACG816_001305"
     CDS             complement(5326..5721)
                     /locus_tag="ACG816_001305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4920189.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ACG816_001306"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ACG816_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4920190.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="ACG816_001307"
     CDS             complement(6198..8390)
                     /locus_tag="ACG816_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HFS4920191.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSCTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="ACG816_001308"
     CDS             complement(8414..9385)
                     /locus_tag="ACG816_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4920192.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10091..11470
                     /gene="brnQ"
                     /locus_tag="ACG816_001309"
     CDS             10091..11470
                     /gene="brnQ"
                     /locus_tag="ACG816_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4920193.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11626..12072)
                     /locus_tag="ACG816_001310"
     CDS             complement(11626..12072)
                     /locus_tag="ACG816_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4920194.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12099..12275)
                     /gene="rpsU"
                     /locus_tag="ACG816_001311"
     CDS             complement(12099..12275)
                     /gene="rpsU"
                     /locus_tag="ACG816_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4920195.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12437..12865)
                     /locus_tag="ACG816_001312"
     CDS             complement(12437..12865)
                     /locus_tag="ACG816_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4920196.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12957..13817
                     /locus_tag="ACG816_001313"
     CDS             12957..13817
                     /locus_tag="ACG816_001313"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4920197.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13901..14251)
                     /locus_tag="ACG816_001314"
     CDS             complement(13901..14251)
                     /locus_tag="ACG816_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4920198.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14267..15994)
                     /locus_tag="ACG816_001315"
     CDS             complement(14267..15994)
                     /locus_tag="ACG816_001315"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4920199.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16207..17133)
                     /gene="trxB"
                     /locus_tag="ACG816_001316"
     CDS             complement(16207..17133)
                     /gene="trxB"
                     /locus_tag="ACG816_001316"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4920200.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17278..18711)
                     /locus_tag="ACG816_001317"
     CDS             complement(17278..18711)
                     /locus_tag="ACG816_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4920201.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18711..20114)
                     /locus_tag="ACG816_001318"
     CDS             complement(18711..20114)
                     /locus_tag="ACG816_001318"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4920202.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20344..21246
                     /locus_tag="ACG816_001319"
     CDS             20344..21246
                     /locus_tag="ACG816_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4920203.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21287..23173)
                     /locus_tag="ACG816_001320"
     CDS             complement(21287..23173)
                     /locus_tag="ACG816_001320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4920204.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23270..24121)
                     /gene="licT"
                     /locus_tag="ACG816_001321"
     CDS             complement(23270..24121)
                     /gene="licT"
                     /locus_tag="ACG816_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4920205.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24579..26363)
                     /locus_tag="ACG816_001322"
     CDS             complement(24579..26363)
                     /locus_tag="ACG816_001322"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920206.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26363..28114)
                     /locus_tag="ACG816_001323"
     CDS             complement(26363..28114)
                     /locus_tag="ACG816_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4920207.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28256..28480)
                     /locus_tag="ACG816_001324"
     CDS             complement(28256..28480)
                     /locus_tag="ACG816_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4920208.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28731..30317
                     /locus_tag="ACG816_001325"
     CDS             28731..30317
                     /locus_tag="ACG816_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4920209.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8550 a         6608 c         5480 g        10050 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct attctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gcgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gctttttttc attgcctcta ctgctttgtg
      301 cagttctttc tccgcttcat tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgca ttcaaaacag cttcgatgac atcatgacga atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgcccttctg ataaaccaat
      601 gccgaaacga tccgtatctt tactgataac atcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat ccgaacgaca ccgtatgctt ggcgacgtaa
      721 ggcataagga tcgtttgacc cgcttggaat catcccaact gcaaagaaag aaaaaatgct
      781 atctaattta tctgcaatcg ctaaaacaga acctactgta gactgaggta gctctccttc
      841 actggatata ggcatgtaat gctcacgaat cgctgttgct acagcaggtt tctcgccttt
      901 cagcagagca tatttttcgc ccatgatccc ttgtagttca ggaaattccc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agctcgttgc agatccatca attcttcctc
     1021 agatagtcct acttgttcgc caatgatacg ggcaatgact tgtacccgct gcattttctc
     1081 atagatacta ccgatttttt catggaaagt aacagttttt agtttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagccgtcaa
     1201 tactttttga ttgcctcgaa ccacattttc caattttacc cgatcaccat tacgagcaga
     1261 aataaaatga ggaagcagca taccttgatc gttccgtacc tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagaaaga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactaaatt gttgacttct tctagtaaat cttcgtctag
     1441 atcaataacc cagtgatttt tttcggctaa ttcatttgct tgtgtaacga tcatttcttg
     1501 tcttttttgc ggtgaagcaa taacgaattg ttcattcaat ttttcttcat attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgtcct cgagaggctc tgcctgtttt
     1621 gacatctaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 agggcggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttctttcgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttcagctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaagc ggcgcggtgt agaaaacgtt tcgatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccgat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt catgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagcacgagc catgttgcgg atacgggaaa
     2281 gataacctgc tcgttctgtt acggatactg caccgcgagc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacgg tcaaagttgc ttagtagcaa gtcttggtca cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacaccat
     2521 cagcccattc cagatcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt gatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtgatctcc atcccatcaa gccatacttc ccaacccaca ccggcacacc
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaag acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtgtcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcgtctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tctgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac accgccagat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt gatccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa atgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aataggtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttcgaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt ttcgatcttc cgccgttttt ctcaacgtgc ggatttttat ccagtttatt
     4381 tctatactta acctataaga agttcagaat atcagtattc gtcttttcgg tagccaaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc gcctttgccg ataatgattc ctttttggct atctctttcg acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgataaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcac tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca agctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac agacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagaaat catgatgtct cctagtacac gaggcaaagg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgcaactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcagt ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgtcgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagca aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgataagt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttgcgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcagatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcggttc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagcacgta ttttgttctt
     7441 tctcctgcat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaatgag tgggaaaatc gaagtcgttt gattcgtcaa tcctagtaat
     7561 tccaattttt tcatcgctga agcatcaatt tgatttcctt cgcgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcagatt gttttaattc ttctgtcttt
     7681 ttctcattca atacatcatt gactacaatg ccttgatcca ctaagtagcg aatcatttgt
     7741 tgctgctctg ttgtgacatt caaatactga atctgatctt ctgcttcttg tttaaaagct
     7801 tccaattcgc tttcacgaac ttgtttactc atctgttcgt caatgagctt caaactttca
     7861 tccctaaccg tgtgaatttc cgtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggcagttttt ggtaaaacgc aacgttttct gcattcacgt tttcaaaatt tttcttcagt
     7981 gtagcaatcc gctcttctgt agtaggtttc gggatatttt catcttcctt cgccttatct
     8041 accttgtcat tataagattt gttgatggta tcattcgtct tatcgatcaa ttcgaataat
     8101 tgcttgatcc gattgttctg gtcatctgcc agatcttttt gataagtata ttctggagtc
     8161 actgcttctg ctgccagttt tcttctttgt tcggtttctt ccgtgttctc gatcgtttta
     8221 ttcgcacgga tactctcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagacaatg ataaaaaaca agaacagaag gaaaggcaat
     8341 gctgcctttc cgagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatgaagat ccgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg atttttttga
     8521 tttgctttag tgttcgttct gcatgaacaa gaccacttgt cgtccctcga ggcaagtcta
     8581 tctgacttgt atccccattg acaatcattt ttgattgata accgagacgg gtcaagaaca
     8641 ttttcatctg agcgactgtc gtgttttgtg cttcgtccaa gataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaatggtg cgatttcgat tacgcctcgt tccatcaatc
     8761 ggttcgtatg atccaatcca aaaatttggt ataaagcatc ataaacaggc cgcagataag
     8821 gatccacttt ttctttcaga tccccgggta gaaagcctag gttttcccct gcctctacag
     8881 cgggccgcgt caaaatgatt ttttgcactt cgcctttttt tagggcagca attgctaata
     8941 ctacggcaag aaacgttttc cctgttccgg ctggccctac accaaatacg atgtcatgtt
     9001 tggctaccga ttcaacgtat ttcttttgac cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtaccttttt
     9121 gccccatttt caatgccgtc acgacatctg gtgtacttac gtggattcct cgtttgatca
     9181 attcctgtaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcaccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttccgt tgtttcctcg atcagtttta
     9301 tatgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagctgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttgt aaaagagtcg
     9481 tttaaccata cgtaccaaga aacgttaaaa aatagctgtt tccattcgct aaaggtctcg
     9541 ttttgataaa aagaatctga gaatgtaagt tatatgaaag tggagaacaa aatattccgt
     9601 ttcattcaga ggtattctct aggtattttt cttgtttcaa aaaaagaaaa caaaatagtt
     9661 aaaatattct tgcatttcaa aaaaaacagg tgtattatta cgtcaacaaa taaacataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt caacgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcaaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt tttttatatt ttaaggtggt ggttttcatg gaatctgata
    10021 ttagtggcat tactaattgc tgcaacgttt atatcgtcaa tcaaaatgat aattattgga
    10081 ggaaaaattt atggaagaaa caaaaactta ttcatggaaa caactattga tggtcagttc
    10141 acttatcttc gggatgtttt tcgggtcagg taatctaatc tttcctgtcc acttaggtca
    10201 aatggctggc gcaaactggg tcagtgctgg cgtaggattt gccatctcag gcgctctttt
    10261 cccattacta gctatcttag ctgtagtagt gacaaaaagt gatggtctgt atgacttagc
    10321 taaacctgtc ggaaaatggt atgctgcctt gttcttaatc cttgttcatt tgacgatcgg
    10381 accatttttc ggtacaccac ggacagctgc tactgctttt gaaatggctg ctaaaccatt
    10441 tttgcctgaa aaatataatt cattaggtat gtttttattc tctgctcttt tctttttact
    10501 tgcttatttc cttactgtcc atcccacacg gttggtcaaa tacgtcggaa agtatctaaa
    10561 ctccttgttt cttgcattat tagcaatcat ttttgtcgtt tctttattcc acccaatggg
    10621 aaatctaaat caatctgctg cagatactta ccaaacaaac gctttaacaa atggtgtcct
    10681 agaaggttat aacacggttg atgcagtagc cttgcttgcc ttaagtgtca cttttgtcca
    10741 tgcagtccgt gaccttggtt tcaaagataa aaaagtcaca gaattaacag caaaagctgg
    10801 tagcctgagc gttgcgctag aagtcatgat ctattttggt cttgttcttc tcggtgcgat
    10861 gagtttgaat aagatgtctt tatcagaaaa tggcggaact gcactttctc agatcactaa
    10921 tttttattta ggtaacttcg gcgctagctt cttaggagtg atggtcacat taggtgtctt
    10981 cactaccgct atgggactcg ttgtttcttt tgcacaagat ttccataaac tgttccctaa
    11041 agtcagttat atgacatggc tgcggttaac gactttcgtc tcttttgtcg ttgcaaatgc
    11101 tggtttggac aatatcatcc aatggtcttt accagtattg atgcttttgt atccattgtc
    11161 gcttgcgttg attttgcttt ccttaacagc aaaatttttc caaaagacac catttgtcta
    11221 tcaagtaacg atgttgtttg ctgctgttcc cgccgtattg gatatgctgg caaattcgcc
    11281 tgctttagtg agtcaacaaa gagttgtagc aagcatgctc gaattttatc atcatcacgt
    11341 tccttttgct gcattgggac ttggctggat ggtcccaacg ttacttgggt atgctggcag
    11401 cttactcttc tattacgcct atcgcctatc agggtataaa caagaagcaa atgaactacc
    11461 agaagaataa ccagatcaaa cacaatttaa cataagaaat attaaacagg aaaaaaacag
    11521 ctagatgaaa gaatcctatt ctttcgtcta gctatttttt agatctcttt tttacgagca
    11581 caaaaaaact gcggacaatg attgtccgca gtttaaatga taaaattaag attgtaatag
    11641 ttctttcaca atggcattta cttggtttcc atctgctttt cctttgactt taggcatgat
    11701 cgcacccatg actttgccaa attctttcat tgatgaagca ccagtttgcg tgattgcttc
    11761 ttgaacaagt tgtcgaattt cttcgtcgga aagttgttcc ggcatgtatt tttcgactat
    11821 cacaatttca ctttttactt tttcagctaa atcgtcacgt cctgcttctt caaactcatg
    11881 caaagaatca cgtcgctgtt tcatttcgcg agacaacacc gttaattctt cttcttctgt
    11941 caagtcgtga ccttctttaa tctgctcgtt ttgtatagat gacttgatca ttcggatgac
    12001 ttgtaaagat tccttatctt tcgctttcat cgctgttttc atgtcatcgt ttaatctact
    12061 tagtagtgac acactaccaa ctccaattca tcacagaact agaatttttt gcgttttcta
    12121 gctgcttcag attttttctt gcgtcttaca cttggttttt cgtagaattc acgtttacga
    12181 gattcttgta aagtacccgc ttttgacacg gaacgtttga agcgacgaag agcatcgtca
    12241 agtgattcgt ttttgcgaac gactgttttt gacatgttaa ttccctccct ccgagcttaa
    12301 aaagtaaccg taattgttat tgaaatatat ctcaaaaaac aagactgtcc tgtaaatatt
    12361 atatctaatg ctgttttgac cgtcaaggaa atcttgcttt tttttaaaat tatcaaatgt
    12421 tattttattt aaatccttat tgacaatttt cacatcttcc gaaaatctcg aatctatgcc
    12481 cttcgatcgt acatccaccg agctgttctt cgaaataatt cattggacac atatggattt
    12541 ctttcgttgt tccgcacacc gtacagataa agtgatggtg atggccgatt tccccttgac
    12601 agcaatgaaa acgaaacttc atctctccat ttaaatcggt ttcttccagt aaatcgattt
    12661 ctgcaaaatc atggagattt cgataaatcg tatcgtaact tactcctttg aattcctgat
    12721 tcatatagtc aaaaacttct ttagcggata cgtagcgatt gcgcttcact aaataagtta
    12781 acaatgcttc tcttttttta gtatatttaa aaccgttatt tttcaatgtt tctagtgatt
    12841 gttcgatcaa cgtctgtttt ttcatgactt attcctccaa atcaaaatga ttacgaagta
    12901 cacattctag agtatggtat aataaacata aaaaaaatgc aagtgaggtt ttatccatgt
    12961 cttccgattc tacagaaatc gttacttttt ttgtcatctc cgactctgct ggagaaactg
    13021 cgacaaaact agcgcaagcc actatggccc aatatcctag cgttgagttc aatttattcc
    13081 gcagaacatt tgtgacagat gagaaaacat tgatgcaggc actgcaagat gcactagaag
    13141 aaaaagctct tgtcctgcac acattgatca atcaagaatt gatcgagatg acgcgttctt
    13201 tttgtgaaga acatcaatta tttagttttg atgtgatgac tccgccgata tcagaaatcg
    13261 agcgcttaac tggtatcaag cccattcggc aacctggcgc ccttcattta ttgaatgaga
    13321 actatttcaa acggatcaaa gcaatggaat ttgctgtaaa atacgatgac gggaaagatc
    13381 ctcgtgggtt tctagaagca gatgtcgttt tgctaggcgt atcccgaact tccaaaacgc
    13441 ctttgagctt gtttttagct aacaaaaatt taaaagttgc caatctacct ttgatcccac
    13501 aagcacatat tcccaaacag ctttgggaga tcgatcctaa aaaaatcgtg ggactgacga
    13561 ataacccaga tattttaaac aatatccgta aagaacggat gcgctcttac ggattaaatc
    13621 ctgatactgc ttattccgat atcgaaaaaa tacgagctga actcgatttt gcgaatgatc
    13681 tttatgaaaa attgggctgc gtagtaatca atgtcgcttc tttatctatt gaagaaacag
    13741 cttcgcttat tttgaatgct ttagacctag aagaccatag ttattatgga actgaaacca
    13801 ccgaagaaac agactaacaa tttttcttta aaaaaactgg actgtgtcag cgaagaagat
    13861 tcgttgacac agtccagttt gtctctattt gcacctatcg ttattgatgt tccacatgtt
    13921 tgagtgtctg gttcaaaata tccagtacgt gagaatcatc aagactatac aaaatcgctt
    13981 ttccctcacg atgggacttg acgacatggt tttctcttaa taaacgcagt tgatgcgata
    14041 cagcagattg ttccatttcc actgcttgga tgattgctgt cacattttgt tccccgtctt
    14101 tcagatacag cagaattttg agacgagtgg ggtcactcaa cattttgaat agttggctga
    14161 ctttctctac ttttgttttt tgcgtgtctg gcaatccatt cacctccaac ttgaaaaaag
    14221 actgacttct aaagtttacc actttttcca tcagtctcaa gtttttttat tcttctgtca
    14281 tcttttggat aatcgtttca taagcggcta ctttttcagc tgcgtcttct gaagagtttc
    14341 cttttacacc gatataaaat ttgattttcg gctcagttcc tgaaggacgg atagcgatcc
    14401 agccattttc ttccagcgta tacttcaata cgtcagatgg cggtgtcgtt agtttttcta
    14461 cgtttccttc acggtctgtt ctcgtcaact ctttgaagtc ttctgtttgg atgacttttg
    14521 ctccggcaaa ttctgttggt gcttcttcac ggaatttttc catcaaagca gagattttcg
    14581 cagaaccttc ttgtccgctc attgtcacag aaatcgtttt ttctgcgtag tagccatagg
    14641 tttcaaaaat cgtttgcaag ccatcataca acgttttgcc ttgtttttta tagtaagctg
    14701 ctacttctgc taataagagc aatgcttgga tcgcgtcttt gtcgcgaacg aaaggtttaa
    14761 ctaaataacc gtaactttct tcgaatccga acataaaagt atgggaatga tcttcttcaa
    14821 attgttgaat tttttcagca atgaatttga atcctgtcaa tacattgaac atggtagccc
    14881 catagtgttc cgcaattgct gtaggtaatt cgctagaaac gatcgatttc aataccgctg
    14941 cgttttcagg taaagtgcct cgcgattggt gtgcttccaa aatatattga gcaaacagtg
    15001 ctccgatttg gtttccggtc aatacttcat aggagccatt tggcaaacga acggctgcac
    15061 ctaaacggtc tgcatcagga tctgttgcaa tcagcaaatc agcgccttct ttctcgccaa
    15121 gtcgtatcgc atactcaaaa gcagagtgtt cttctggatt tggtgatttg actgtagtga
    15181 aatctgggtc tgcgacagct tgttctggaa ctagtacgaa ttgttcgaat ccagcttgtt
    15241 tcaacgcacg ttcgcccaac atttttcctg ttccgtgaag tggtgtgtac acaagtttca
    15301 aatctttccc cattgtttcc accaattcat gatcaatcgt tactgctttg atttcttcta
    15361 gataagcggc atccacttct tctccgatga tcgtgatcaa tccgctatgt tcagcttctt
    15421 cgtcagataa tacttcaact tttaacggat tgctgacttc acggacatat ttcgtcaatg
    15481 catctgcgtc tgcaggaggc atttgtcccc catcttctcc gtagacttta tatccgttgt
    15541 aagctgctgg attgtgggat gcggttacca tgattcccgc aaacgtcttc aaatgacgga
    15601 ctgcaaaaga aagctctggt gttggacgta gactttcgaa tacataagat gggatattgt
    15661 gatgtgctaa tgttttggct gcttccatgg caaattctgg tgattgatgg cgtgagtcat
    15721 aagcgatagc gacacctcgg cgttttgttt cttcgccttg tgtatccatg aaacgtgcaa
    15781 gtccttctgt tgcttgtctt accgtataga tgttcattcg attgatccca ggccctaaga
    15841 ttccccgcat gcctgcagta ccgaattcca acggtgcata aaaagcatct tcaagttttt
    15901 caggatcttc acccagatca tttaattgtt tttttaagtt ttcttctaaa ttttcttcat
    15961 gtacccattg ttcatacact tgttcccaag acatgaaaat aacacctctt tctgaaatat
    16021 ggttcttaat aagtatagca ttcacttatt agatatgaaa gtatgaggac caaaaaacgc
    16081 ttaaatcaag aaaggattct tttatcaagc acggttgcaa gtattcgcat aaatcggatt
    16141 cagtagattt cacgcgaaaa aaaagagtaa aagaacaatt aaaccgttct tccactcttt
    16201 ttaaacttat gcagttgttt cttctaaaga ttccaagtat ttatacactt gttgtccagc
    16261 gatcccacca tcaccaacag cagtagtgat ttgtcgcaaa tctttttcac gaacatctcc
    16321 gatggcaaaa acgcctggta tattcgtctt catttcgttg tctgttggaa tccagccagc
    16381 ttcgttcgtc aaccctgctt ctctaaatgg tgcagttaat ggatccagtc caacatagat
    16441 gaatactccg tctgctgcca aatcaaactc attgccggtt ttgacattac gtgctttcac
    16501 acctgtcact accatttcat tcccaacgat ttcgtcaacg actgtatccc acataaagga
    16561 aattttttca ttcgcaaatg cacggtcttg gatgatcttt tgtgcacgta attcatctct
    16621 acggtggacg atgactactt cagaagcaaa ttgtgtcaaa tagatggctt cttctactgc
    16681 tgagtctcct cctccgatca ctactagacg tttgttacgg aaaaacgcac catcacaaac
    16741 tgcacagtaa gaaacgccac gtccagcaaa ttcttcctcg ccaggtacgc ctaatttacg
    16801 gtggatacag cctgttgcga taatgactgt ttttccttcg taagttttat cttcacaaat
    16861 aacttctttg tggctgccgt gatccttgat atcttgcacg attccataag cattttctgt
    16921 cccaaatctt gaaacacctt catacatctt ataagctaga tcaggaccca ttattgaatc
    16981 gaaaccagga tagttttcga tttctgccgt attgttcatt tggccgccag gggcgccttg
    17041 ttcgatcatc aatacggata agtttgaacg tgaagcatat aaagcagcag tcatcccagc
    17101 aggacctgcg ccaataacga ttacatcata catatgtaga ttctccttta cttaatcaag
    17161 taagcatact ttacagccaa tgcgaaaaaa agtctattgt tctgcttgcg tttctcgaat
    17221 agctttatta tactcgaaaa atccgctgaa aaacaataga cttttataaa tcattgatca
    17281 atcattcgaa agattttctc catttgaatc aattactttt ttataccagt tgaatgattt
    17341 cttcttcgaa cgggctaatg tgccatttcc ttcgttgtca cgatcgacat aaatgaatcc
    17401 gtaccttttc ttcatctcac ctgtaccagc tgatactaaa tcaatacatc cccaagtcgt
    17461 atagcctaat agatcgactc catctaattc tactgcatct ttcattgcct ggatatgtgc
    17521 tgccagatag tcgattcgat agtcatcttc cacataacca ttctcatcgg gcgtatcgac
    17581 tgcacctaag ccgttttcta caataaatag tggtttttga taacgatcat aaatatcatt
    17641 catcgttatt ctcaagccta atggatcaat ttgccaaccc cattcacttg cttccagata
    17701 tggatttttg acagaagcaa agatatttcc tgcagtctgt tccaagagtt cgttgtcagt
    17761 agtcgaaact cgagaagaat aataagaaaa ggagataaaa tcaacagtat gttctttcag
    17821 caatgcggca tccccaggtt ccattttgat ctgaatgcca tttcgttcca tttctttcaa
    17881 tgcataagca ggatattctc ctcgtgactg gacatcaatg aagaaataat tttctcgatc
    17941 tgattttcta gaagcccaaa catcttcagg attgcaagtg tatggataat atgctcctgc
    18001 tgccagcata cagcctactt tgttctccgg atctacttca tgagcaatct tcgttgcgat
    18061 ggcacttgca accaactcat gatgtgccgc ttgatatttg atttgttctt cattttctcc
    18121 ttcttcaaaa tacagccctg cgcccatgaa tggtgcatga aggatcatat tgatctcatt
    18181 gaatgtcaac caatatttga caagtccttt atagcgattg aagatgactc ggcacagatt
    18241 ttcataaaaa ccaacgagtt ctctggaacg ccacgcacca tattctttga tcaaatgcat
    18301 cggacaatca aaatgtgtaa tcgtcacaag cggctcgatc ccgtactttt gacattcttt
    18361 aaagacatcc tcataaaatt tcaaaccaga ttcatttggc tctttttcat cgcccattgg
    18421 aaaaatacga ctccaagcaa tggacatccg ataagtcttg aaccccattt ctgcaaataa
    18481 agcaatatct tccttataat gatgatacat atcgatcgca atttttgctg gataaaaatg
    18541 attttcatca aattcaaaca ttttcttttt tccagtaatg acaggaaaac ggtcttctcc
    18601 gattggcaca acatctacgt tcgctaaccc acgtccgcct tcatcataac cgccctcgca
    18661 ttgattggct gctgtagctc cgccccataa aaaatctttt ctgaatccca tcatacttcc
    18721 tccattacat gtgtattgct tgataattct cttgccagtt catcttggac ttcgattcct
    18781 tctgctttta accggctgat tgtttccttg aattgcggta aatactgctg atgtgcaata
    18841 aacaactcat tcatgatccg ttttgctgtc tttcctgatc gtataagcgg attgatcgta
    18901 aacgcttgta aagctgttcc ataatcacca gtcacagctg cttcgatcgt cagcaattcc
    18961 atattcttca ttacttgcaa ccagcctctt tcagcagcag gaagggaacc aaacgctaca
    19021 tttcgtgcgc cttgtgcgcc aacataggca gtgacttcta ctacacaatc tgccggcaga
    19081 tcaggaacag ctccatcatt ctttgtagat accacgatct gtgtttcttt atttccatag
    19141 atcgaagcaa tcgtttcaca ggcagcatct gaataataag caccacctcg tttttgcaat
    19201 tgttcaggtt tatgatccaa ttctggatct ttgtataatt caaacagctc tgcttctgtt
    19261 tctttcactt gctgcgctct tgtaccaatc gtttgatatt cttctaaagc atgttccagc
    19321 atttcttctt cacggtagta atagcggtgg tagccacaag ggatcatgtt catttgtttt
    19381 aattgttctt tgaaaaacgg gatatcaaag atatttttcg gcaatccatt gtcttcatcg
    19441 tataaacgat caatcaattc tgccgtgcgg tcatttcctt tattatctgc tactttgtgc
    19501 caatggaagt gatttagacc ggcaaacttg tagatcagct cttctttttt cttgccaagc
    19561 attgttggtt ctgtcatcat cgccattact ggcacattgc ataatcctat cactttatcc
    19621 catttgccat atctgataac tgcttctgtc accatcccgc ttgggttcgc gaaattgatc
    19681 agccaagcat ttggacataa acgcttcatg tcttcaacga ttgccaaaac cactggaacg
    19741 gtacgaaaag ctttgaatat cccgccagca ccatttgttt cttgcccaat catcccataa
    19801 taactaggga tccgctcgtc tttgattcgt gcatctaatt gaccgacacg aaactgagtc
    19861 gtaacaaaat ccgcatcttt caatgcttct tcccgatcca gagttgcata gattttgact
    19921 tcatacggag aagcatccca catacgctga gacatctgac tgacgatctc aagcttttct
    19981 tttccttctt ctacatctac gagccagatt tctcgtatcg gtaattcctc atatcttttg
    20041 ataaatcctt ccatcaactc gggggtgtaa ctgctgcctc caccaatcgt agcgattttg
    20101 attccttttg ccatgttctc actcctttac tttttacact tttattatct tttttgtaaa
    20161 caaaacaaac aatagaaatt cgtttctttc cagtaacatt tacaaaagtt agtgttttct
    20221 actggatttt tgtaaatatc ttgtcaaaaa atgtctttat taagctatat aacataaatc
    20281 cgtttacaaa tcattttcaa aaaagttata atttacgtaa aaaaacaaga agaaggtatc
    20341 tacatgttat tacgtgaaaa aatcaaagaa atctcctttt ctccttcaga aaatgaagtg
    20401 attcagtttc tcttaagcga ttctccagca cttggcgaaa tgaccatcca agagattgct
    20461 gagcgatgtt atgtccaccc gtccacactc atccgtgtag caaaaaaaat gggttttgat
    20521 ggctgggtca gttttcgtaa tgctttttta gctgaaacag cctatcttga agggaatttt
    20581 caagacgtag atgcgaatct tcctttttcc accacagacg gtattatgac gatcgcaaat
    20641 aaaatggctt ctttagaaca gatgacgatt caagatacac tttctctcat tcatcatgac
    20701 caactgcaaa aagccaaaca actaatgctt caagcaaaaa aaatcgtgat tttcgccagt
    20761 aactcgaata ctttgatttc tcaagatttc atgctgaaaa tgaagcgcat caaaatggat
    20821 gtcacgattg tcaccacttt tggtgaatcg ttctatgaag cgtacaattg cacgagtgat
    20881 atgtgcggga ttctgatttc ttatacgggt gaaaacaatg tgatccttcg taccgcagaa
    20941 atattgaaac aaaatcatgt tccaatttta gctttgacca gtatcggtga ttcaacgcta
    21001 tcttcaatga gtacagccgc tttgcgactt acgacacgcg aacgactgta ttcgaaaatt
    21061 ggtaatttta cgatcaatac atcgatttgt tatcttctag acgtgttata tagttgtgtt
    21121 tttgcagaaa attatcaaca gaatttagtc catctaattt ctgtaggaca aaaatttgat
    21181 aaacgcccta tcagctcagc catcatgaaa gagcctttca attttgaaaa taaagaagtg
    21241 gactaaaaag aataattaag cctagtccat tctttcacct aaccgttcag gtcagtgcgg
    21301 tgataagtgc atcgtctggc ccgaccgtct ccccttgatt ttccaaaata tccaaataat
    21361 ccgtgtaatt ggtgacgatc acaggtgttt ctaaactgta tccttcctct tcgattttct
    21421 ttttgtcgaa gcggagcaac agatcgcctt gtttcactct ttctccttgc tgcaccaagc
    21481 tttcaaaata tttcccttcc aactgaacag tatccaatcc gacatgaatc agcaattcca
    21541 gacctgcttc agaaaccatt ccgattgcat gtttcgtcgg gaacagggtc atgactgttc
    21601 cgtcaaaagg ggcacgtact tctccttttt cagggtagat caagattcct cgacctagtg
    21661 ttccttgtgc aaaagctgca tcctttgctg cattcagtgg agcgatttgt ccttgtatcg
    21721 gtgtttttac aatttcttta cctgatcttt tctgtctatc atctgtttca cctgcttcct
    21781 ccttccaaaa gaaaaaagtc agcgcaaagg ataccaccat cgcaatggcg attccaatca
    21841 atacattaat cacatccgtt aaatcaccat ctggtctaat atagttggga attgtaaaga
    21901 tgcctaatcc acctggattc caagaagtta aattcattaa tccactatac gcgcccccta
    21961 acgcaccacc gacacaggta aaaataaata tttttttacg tggcaatgta aacccataaa
    22021 tagctggctc tgtaatcccc acaataccag aaacaatggc tggtattgcg attgctttgt
    22081 cttttgggtt tctaagctta aagtagagtg cagtaagtgc tgcagtttgt ccaaaagaca
    22141 ctactgcaac aggtgtcatg atattttgcc aaccattcac agaaaattgc atcaatccca
    22201 ttggtacgat tgcccaatgg agaccaaaca tcactagaat ttgccagaag aagcctacga
    22261 ttgctccata aataactggc gaaagtgcta aaagtgtttc aaatccagcc attaaaatat
    22321 cagttgcgaa tgtgaatgct ggtccaacga gtaaaaaacc taagggcatt gtaataagaa
    22381 cggtgaagaa tggaacgaag aaattcgcga tcatctcagg tactaacttt ttaattggtt
    22441 tctgaatttg gctagctaac aacacaataa agataatggg aatgacagaa gaagcgtagt
    22501 tacgcgcaat ccagggaatg ccgaatactg tttgataaat aggtgcttca aaaatagtgc
    22561 cagaaaatat ggtagtcagt ggtgcttctg ctgcaccact caaagcttcc agcgacaagc
    22621 taggattcat taatgctgcc ccaagcaaca tccctacgaa ttcatttact ttaaatttac
    22681 gggcagctgt taaaccgata ataacaggca taaattggaa tacagaatca ccgatattat
    22741 caaacataac aaatgtgcca gaaccgcggt caaatgcgcc taagaagaca agtaatgctg
    22801 tcagtccacg aatcatccca ccagcagcta gaatagcaag gaatggctgg aaacagcccg
    22861 atattccatc aataagcata tcaaacggtc tcattttttc tgttgaaaca gcctcatcaa
    22921 aagtaaggtt tcctgctttt ataacctctt caaacacgac tggcacatga ttcccgatga
    22981 caacttggta ctgtcctccc gctcgcatta ccgtcacaac accgtccata tttttcaaga
    23041 tctcatcatt tgctttttct tcgtttttta atttgaaacg aagccttgtg atacaatgtg
    23101 tcaaaccatt gatattttcc ttcccgccga cattttctac aattttgctc gctaattctt
    23161 gatattttcc catttctatt ccctccaatt tattagtgga aggtttagcc aactgaatgt
    23221 cactaccctt atttcatgcg ttcttgttaa tttatcctcc tttttttatt tatttacaac
    23281 gattctcatg cactaaacgc gcaatatgga tcgttaaata cattttttcg tccgtggaca
    23341 tctggtagtt gaatatctcg gctaaaaagt ctgctatttt ctcaacgcaa ccaaacgtat
    23401 ccttatattt tttttgaata acctggtaca attcctcaga aacttctccc acttctgatt
    23461 tttctccatc taggacgcgt gtcagaaaaa aacgtaggtg tgtgacgaat cgaaaaaaat
    23521 ggactgaatc ttcatcaaat gtaatcttat agtaatagcg gacgatattt agaatatcct
    23581 gtattgttgt agtaaacagg taggtattgt cttgttggct ttgaatgcct tgtgcattga
    23641 ctaaatgcaa tgcgataaat ccggcttcat cttctgataa cttgatgtgg tatttatccg
    23701 agattttctt cactgttttt ttcccgatgg catactcttt aggaaatagt tgtttgatat
    23761 catacagcaa tacatttggt acaaatattc ccattttgtg acgttcgata gccgtataca
    23821 aatgatccgt caatgcaata tacaccgaat cattcaactg gacttgtaga gcctcttttg
    23881 tatattcaat gatttcacaa gaaatttcta agtactcaag aggaatcgct tgtgccaatt
    23941 cttggaactg ttgtgaaagt ccgtcatttg tcaactgaaa ttttttgtcg atgcatgagt
    24001 caaaaatcgg gtcacccact ttctttttga atgccaaacc ttttcccatc acgatcattt
    24061 cctgatttct ttcatccctg gttattacga cgttgttatt cagtactttc tcaatcagca
    24121 tgttttactc ctttccatga tatatagggc tgtatccaaa acaaaaagaa aaacccaaaa
    24181 agccgtccat ggaaccatgg gagactcatc aggtttagct tgcatcaggc aatcactatc
    24241 caagttgtat cgttttcttt atattatcga aagcaagcac ttttgtcaac gctttcttag
    24301 cggctattgg gcacttctga ctagctgtcg tgattttttt taaatctggt aattacgaca
    24361 ctaacgctca cagatgaaga atctgggaag ctctgtttta ttttcactct agcgcttagg
    24421 tcaaaatgct actatgctac tgtcccaact tctaatatac ggtgttccag taccagctta
    24481 tcggtgataa gccaaattta tgcaaaaatc tggaaagcct tttttacaat ttttttctta
    24541 tcacctaaag ctgaacggag ccctgtcaca accacttttt acacaccact gctttgcagt
    24601 ttgtacatat ctgcatacaa accattttgc gctagaagtg tttcatgatt cccacgttct
    24661 actatatgac ctttgtctaa aactaaaatc aattctgcat cccgaatcgt ggacagccga
    24721 tgggcaatcg caatcgttgt ccgtccttgt cgcatttttg tcaatccttc ttggatcaat
    24781 ccttctgttt cagtatcgat atttgctgtc gcttcatcca atactagtat tttaggatct
    24841 gtcacgattg ttcgcgcaaa agtgatcagc tgtcgttgtc cgctagaata gctggcaccg
    24901 tgttcgatca cttttgagtg atatcctttt ggtaattcat cgatgaactt gtttgcttga
    24961 acaaactcag ctgcatggcg aatctgttcg tctgtgatag ttggattcat catccggata
    25021 tttcctgcga tatctccata aaacaagaag gcatcttgca agaccaatcc cattttctta
    25081 cgtaactctt ccattggata ttctcgaatg tcatgatcat caatcaagat ctggccttca
    25141 taaaattcgt aaaaccgcat caatacgttg atgatcgagc tttttccgct tccggtgtgc
    25201 cccactaatg caacggtctc tcccggatta gcgacaaaag agatattgtt caatacattg
    25261 tgctctccat cataagaaaa gcttacattg cgaaactcga tcttacctga agtaatcgct
    25321 tgatctgctc caggattttg ttgcggtgca tattcttctg tatccatgat tttcaaaata
    25381 cgactaccgg caaccattcc atctgtaaag gtgctcaaaa agtccatcat ttgagacatt
    25441 ggattaaaaa atccctggac ataggtcgta aatgcataga tcagtccaac ttctacggga
    25501 gaatgcagcg catcgatccc aaacaaagtc aacgccaacg caatcgccaa tgcatataga
    25561 aaattgatga tcggacttaa cagaatcgaa tttgttttga tcatcgcata gcgcgtgtct
    25621 aaatactcgt tgttcacttg ttcaaattct ttagctaaac gagcttcttg gcgaaactgc
    25681 tggataatac gcatgccgga aatcgattca ttcaatttcg tgttcagctg acttaatttt
    25741 tcccgcatat tccggtaaat acgagaactg aatttctggt aataccagat gacgattcct
    25801 aaaatcggca agaacaccaa agtccacaag gcaatttctc cattgatttg aaacatggca
    25861 atgaaggaag aaacgactgc aaagatccca gtcaatacca tcaaaaagac ataccaaaat
    25921 tcaaatagcg tttctgtgtc atttgtcact cgagaaacag tcgatcctgc cggtgtctga
    25981 tcaaaataac gcatacctag tgtatgcagc ttttcaaata atcttacccg aacatactga
    26041 taggtcttca atgaagccat ggaatacaaa taccattgga aaaaccaaat gatacttttg
    26101 actatgactc caaacaagta cataccagca aaaaacaaaa taacttgtgt cgtagccgac
    26161 tgtttagcca gatgatcatc cataaaagtt tggatgatcc ttggcaatag gacattgatc
    26221 acagacaggg caaacgcaaa taagatagca acgatgaacg ttcttctaaa aggtttggcg
    26281 aacttcatca gtcgtttgat gatcttcttc tgttctttaa gtggaatcga ttttgtccat
    26341 tccgattgat aattttcatc catcaggcct cgcctccttc tatcttcgct tctaatttct
    26401 gtttctccca cattcgttca taccagccgc ctaatgcaag cagctcatga tgggttcctc
    26461 gttcaatgat tttcccttga tcgacaacaa tgatttcttt tgcatgcatc acgctgctta
    26521 aacggtgagc tgcaatgatg gttgttttct cttttcttat ttcttttaaa ttatgcaaga
    26581 tagcttcttc tgttttggca tctactgcag aaagtgcatc atctaagatc agcaattcag
    26641 gatttttgat caatgctcga gcaatcgaaa tcctttgttt ctgtccgcca gataaagaga
    26701 ctccacgttc tccaactaag gtatcataac cttttggtaa ttccttgatt tcattgttga
    26761 taaatgctaa agaagccgca tgctctactt cttcttctga aagttttgga ttagaaaaac
    26821 ggatattatc tcgaatggtc atcgaaaaca agaagtgatc ttgaggcaca tagccgattg
    26881 atcctaataa agcattcaat gaatattctt tgatcgaaca tccgccaaat tgaatttttc
    26941 cttgataatc atcgtattca cgcatcagta atttgatgat tgtcgttttt cccgcaccag
    27001 ttttcccaac aatacctaat gtttctcctt cttggatcgc aaatttgatt tgttctaatg
    27061 ctggtgattc ttctttcgga tagttaaaag aggaaatagc tacattcagc tcgccttttg
    27121 ccggagtctg cactgcaccc agttgttcca cgatatgcgt cttttcatgc aaaagttcat
    27181 tgacgcgatc ataagaagca tttcctcttt ccaatacgtt gaacaatcga ccaatcgcaa
    27241 acatcggcca aacaagcata ccgatatagc taataaatga gaccatctgc ccgattgaga
    27301 tcgtgccttc caagacatac tttccaccta aaataattgt tgcaacataa gacagaccga
    27361 taatcaaggt aatgaatgga tcaaataagg agtctaaaaa attgactcgt ttgtttttaa
    27421 taattgcatc ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct tgtccaaatg
    27481 ttttaatgac tttgatccca gtaatacttt cttgtgtctt atcattgata ctagaaaaag
    27541 cttcttgcga atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt gaagtaacgg
    27601 ctagtaaagg cagaggaatc agcgcaatca gtgttaagcg ccaatcaata aacaaaatca
    27661 tcgcaatgat cgttgttccg cccgtgatca ctgaatctgc aaaggtcaag ataccagctc
    27721 ctgctacatt ttggattgca ttcaaatcat tcgttgcatg agccatcaaa tctccagtcc
    27781 gatatttttg gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc tgctgccgaa
    27841 gttctttctc caaccgagct gcacttcccc aaatattcgt ccgccagata taacgaaaaa
    27901 gatattgtaa gagagctgca aggatcaata ataagatcca ccaggaaatg atctttattg
    27961 ataaattttt atcggcgatc tcatcaatga tgatcccaat cacttttgga ggagctaact
    28021 gtaccaaggc cactgcgaat agggagaaaa tcccaattaa ataatgtttc ttttcctgtc
    28081 gaaaaaacca acctaatttt ctaaaaatcg acattttact cctcctttgt ctttatcaag
    28141 agcataggat tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc taacaaatta
    28201 cgaaataaaa aacggactga aaaaatcagc ccgcaaaaag tctttgggtc agaacctatt
    28261 ttttcccttg gttcttcatt tgctgcatca tctggcgaat tttcttctca gatggttttt
    28321 gccccattga catcatcatt gatcgcaaca tgtcctcatt aacgggagga tttttttcta
    28381 aatagtcttt catatattta cgcgctagga aaaagccacc tactgcgccg attagcaaag
    28441 caataatagc aataagtacg acaatacctg ttgataccat ataaatctct cctttctatt
    28501 tccgaattgc tctcaatgat ttattttact aaagtttatt gaaaaaagaa aggcgtattc
    28561 tttagtttca tgcaaaaaat agtggttttt ttagagaaga acaaaatttc tttcattaac
    28621 cagcaagaca cgccacgaaa aaaaattagg cataccaaat ctttttcttt aattttttct
    28681 ttctgaggtg tagaatgtaa tcgtagataa ataggaaagg tggttttttc ttgaataatc
    28741 aatccaacaa acaatttcat aagcatcgac ttattgaaca tacaaatggt ttatctttag
    28801 ctgaagtaaa tggaacagtg gatgtaccca agggaaaaag tttttttaaa acacttcttg
    28861 cttactctgg accaggtgca ttagtagcag ttggttatat ggatccaggt aactggtcca
    28921 cttcgatcac tggtggacaa aatttccaat atttattgat gtcagttatt ttgatgtcta
    28981 gtctgattgc tatgctttta caatatatgg cagcaaaatt aggaatcgtg acccaaatgg
    29041 atttggccca agcaatccga gcacggacaa gtaagacatt gggtgtcatt ttatggatct
    29101 tgactgaatt agccattatg gcaacagaca tcgcagaagt cattggtgca gcaatcgccc
    29161 tctacctgct ttttgatatt ccattggtaa ttgccgtatt tatcactgtg ttagatgttt
    29221 tcgtcctttt attgctgaca aaagtcgggt ttcgtaaaat cgaagctttg gttgtctgct
    29281 tgattttcgt cattttattc gttttcgtct atcaagttgg cttgtctaac cctgattggc
    29341 acagtttgtt tgccggattt atccctaaca gtcacacatt ctcagactca ccggtcatcg
    29401 gcggcgaaac tccgctgact ggagctttag ggattattgg tgcaacagtc atgccgcata
    29461 acttatatct tcattcagca gtttctcaaa ctcggaagat cgatcaccaa gacgaagaag
    29521 atgtagcaca agcggtacgc ttttctactt gggattctaa tatccagttg tcaatggctt
    29581 ttctagtcaa tgcactgcta ttgatcatgg gtgtggctgt attcaaaacc ggttctgtcc
    29641 aagatccttc cttttttggt ctgtatgaag ctttatctga tccatcagct atgagcaatg
    29701 gtatcttgat tgctgtagca aaatctggta tcttgtctac tttatttgct gtagcacttc
    29761 ttgcttctgg ccagaactct actattactg gtacgttgac cggtcaagtc atcatggaag
    29821 gcttcatcca tatgcgtatc ccaatttggc taagaagact agtcacacga ttgctttctg
    29881 tgattcctgt attgatttgt gttttgttta caagtacaaa aggaacgatc gaagaacata
    29941 ccgctttgaa taacttgatg aatgaatccc aagtttttct ggcttttgca ttgccattct
    30001 cgatgattcc tttactgatg atgaccaata gccaagcgga gatggggagc cgattcaaaa
    30061 attcatttgt tgtaaaacta cttggctggt tctctgtgat tagtctgact tacttgaatt
    30121 tacgaggatt accaggtcaa atcgaaagtt tcttcggcga gcaagccacc tcagctcaac
    30181 tagcattggc tgatcaaatc gcttatgtaa tcattgttct tgtccttctc ctgttggcat
    30241 ggacgattgg tgatttatat aaaggaaaca aaaaatatga acagcgtttg caggaaacga
    30301 tagaagaaat cgagtagaaa ctgattcttt tacacaaagg aggttgtgtc agaagtgggc
    30361 agcttcaaga aataagaagg aatttaagaa aattgctttt caaatttttg tgaaatttcg
    30421 gcttatttcc gaagaagtta cttctggaac accgtgtatc agaggttgtg aaaaagctgc
    30481 cttgcatcaa gtaataagaa cagccaagta aaaacagctt tttatgtttt taacttggtt
    30541 ggacttatta ccgcagatgc aagcttttga gcaccgttta ttcggaaaaa ggaactgcga
    30601 caagactttt gtcacagttc cctttcctac gattagtcaa gtaaaaggtg taagaaaaat
    30661 agctcataaa cgaaagcgac aaaatcaa
//
LOCUS       DAZUKT010000030        27862 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000030 DAZUKT010000000
VERSION     DAZUKT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.030"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..2088
                     /gene="yfcC"
                     /locus_tag="ACG816_001326"
     CDS             349..2088
                     /gene="yfcC"
                     /locus_tag="ACG816_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4920210.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2245..3429
                     /gene="iadA"
                     /locus_tag="ACG816_001327"
     CDS             2245..3429
                     /gene="iadA"
                     /locus_tag="ACG816_001327"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4920211.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3540..3743
                     /locus_tag="ACG816_001328"
     CDS             3540..3743
                     /locus_tag="ACG816_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4920212.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3844..4506)
                     /locus_tag="ACG816_001329"
     CDS             complement(3844..4506)
                     /locus_tag="ACG816_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920213.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4519..6021)
                     /locus_tag="ACG816_001330"
     CDS             complement(4519..6021)
                     /locus_tag="ACG816_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920214.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6569..8248
                     /locus_tag="ACG816_001331"
     CDS             6569..8248
                     /locus_tag="ACG816_001331"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4920215.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8375..8854
                     /locus_tag="ACG816_001332"
     CDS             8375..8854
                     /locus_tag="ACG816_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4920216.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9073..9753
                     /locus_tag="ACG816_001333"
     CDS             9073..9753
                     /locus_tag="ACG816_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4920217.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9761..11092
                     /locus_tag="ACG816_001334"
     CDS             9761..11092
                     /locus_tag="ACG816_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4920218.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11107..12069
                     /locus_tag="ACG816_001335"
     CDS             11107..12069
                     /locus_tag="ACG816_001335"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4920219.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12180..12518
                     /locus_tag="ACG816_001336"
     CDS             12180..12518
                     /locus_tag="ACG816_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4920220.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12597..13304
                     /locus_tag="ACG816_001337"
     CDS             12597..13304
                     /locus_tag="ACG816_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4920221.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13407..14888)
                     /locus_tag="ACG816_001338"
     CDS             complement(13407..14888)
                     /locus_tag="ACG816_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920222.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15197..15733
                     /locus_tag="ACG816_001339"
     CDS             15197..15733
                     /locus_tag="ACG816_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4920223.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15811..17181
                     /locus_tag="ACG816_001340"
     CDS             15811..17181
                     /locus_tag="ACG816_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4920224.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17248..17583
                     /locus_tag="ACG816_001341"
     CDS             17248..17583
                     /locus_tag="ACG816_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920225.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17664..18521)
                     /locus_tag="ACG816_001342"
     CDS             complement(17664..18521)
                     /locus_tag="ACG816_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920226.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18628..19143
                     /gene="tadA"
                     /locus_tag="ACG816_001343"
     CDS             18628..19143
                     /gene="tadA"
                     /locus_tag="ACG816_001343"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4920227.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            19166..19250
                     /gene="ffs"
                     /locus_tag="ACG816_001344"
     ncRNA           19166..19250
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG816_001344"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19460..20845
                     /locus_tag="ACG816_001345"
     CDS             19460..20845
                     /locus_tag="ACG816_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4920228.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20882..21559
                     /locus_tag="ACG816_001346"
     CDS             20882..21559
                     /locus_tag="ACG816_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4920229.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21567..22331
                     /locus_tag="ACG816_001347"
     CDS             21567..22331
                     /locus_tag="ACG816_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4920230.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22333..22992
                     /locus_tag="ACG816_001348"
     CDS             22333..22992
                     /locus_tag="ACG816_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4920231.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(23022..23531)
                     /locus_tag="ACG816_001349"
     CDS             complement(23022..23531)
                     /locus_tag="ACG816_001349"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4920232.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23643..24641)
                     /locus_tag="ACG816_001350"
     CDS             complement(23643..24641)
                     /locus_tag="ACG816_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4920233.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24842..25426
                     /locus_tag="ACG816_001351"
     CDS             24842..25426
                     /locus_tag="ACG816_001351"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4920234.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25503..26678)
                     /locus_tag="ACG816_001352"
     CDS             complement(25503..26678)
                     /locus_tag="ACG816_001352"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4920235.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26760..27140
                     /locus_tag="ACG816_001353"
     CDS             26760..27140
                     /locus_tag="ACG816_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4920236.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27214..27576
                     /locus_tag="ACG816_001354"
     CDS             27214..27576
                     /locus_tag="ACG816_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920237.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9085 a         4688 c         5796 g         8293 t
ORIGIN      
        1 ataaaataca caaaaattga aggatagata atggatattt ataagtgttt attatttttt
       61 ttgcaatgaa attcaatttt tcaatgattg taaaatgaaa aaaacaggac gagattttct
      121 ctaatctaaa aataaaaaaa gaattttaag gaaaaacaga gaaataaaat acttaatata
      181 aatggaaaga gaaaaaaata atcatacata ggagaaaaaa aacaaaatag aaaaaagtga
      241 ttttctaata aattattcat gtggaaatgt catttttact gtataattca tttgattatc
      301 cattttttat tcatcaaaat tactgaaaaa agaaaggaag aaataaattt gaagaaaaca
      361 aattcttcag gaaaaccgaa acgcgacttc actaaattat cgacacccca tacgtatgtc
      421 attatttttg gtgtagtcat ctttgcatgg atactgactt ttgtcgttcc agcaggaaag
      481 ttcagcacgc aagatattga atacaaagat gcaaatggcg aaacaagtac tcgtactgtt
      541 ttacgtcaag attccttccg ttatgcttac gagctggata agtcgtatgt gtttgatcaa
      601 ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac tggatgttcc ggaaaaagga
      661 ttggaaaaag tcattgctga cggagaaaaa aatcttacgc aggaaaaact agatgaaatc
      721 tccttgacag atgatgtgct ttacgatcag tacggcgaaa atatctatga tacttctaaa
      781 aaattgcata aaacagccaa aatatgggga acagatgatt ttggcggctt tggtttctta
      841 aattttgttt ttgaaggact agtatctgga gataagtatg gatctgctgt agggatcgct
      901 gcattgatcc ttgttgttgg aggtgctttt ggtattatta tgcgtaccgg agcgatcgac
      961 gcaggtatct atgcgtttat cagtaaaact aagggactgg agcgtttagc tttaccgcta
     1021 ttattctttg ctttttcatt cggtggcgct acattcggga tggcagaaga ggtaattcca
     1081 ttctcaatgg tgatggttcc atttgtcatt gcgttaggat atgactcgat cgtagcagta
     1141 accgtgactt atgtcgcatc tcaagtcgga aatgcgacct cttggatgag cccgtttagc
     1201 gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt ctggtgcgac attccgattg
     1261 attatgtggg tagttgttac tgcattagcc gctggttata tgatgatata tgcagaaaaa
     1321 atccgtaaaa aaccagaaag ttcgttgact tacaaatcag atgattattt ccgttctcat
     1381 atcaaaaaga catctgatga aaataaaaaa ttcatgcttg ggcataaatt gatcttatta
     1441 gagatgttag ttgtccttgt ctggatcgtt tggggcgtga ctcaaaaagg ctattacatt
     1501 ccagaaatcg cttctcaatt ctttgtgatg ggattagttg ctggaatcat tgctgttctt
     1561 ttcaaattag atggaatgaa agtcaatgac atcgcttctt ctttccaatc tggtgcagct
     1621 gatttagctg gtacagcaat cgtagtaggt atggccaaag ggattcttct tgtcttggga
     1681 ggatctgatg catctgttcc ttcagctttg aatacgattt tgcatgggat cgggacggcg
     1741 ttgaccggtg ttccagctgt cattggtgct tgggcgatgt acatcttcca aagtttgttc
     1801 aacttggttg ttacatcaaa ttcaggacaa gcagctttga ctatgccaat catggctcca
     1861 ctggcagatt tagtaggtgt atccagacaa gtagcagttc ttgcttatca gcttggttca
     1921 ggttttatgg acgcttttac acccgtatca gccagcttga ttggtgtgct aggtgttgca
     1981 cggatcgaat gggcaaaatg ggcaagattc caaatcaaga tgcaaggatt cctgtttgtt
     2041 ttaggaacta ttttcatcat gattgccatt gcaataggtc tgcagtagta ctggagacct
     2101 gtaaataaaa aagtaatcca aacagtagat aaggactcca agaaccgtca ttttacggta
     2161 aaatcaaagg agtaaagatc ctatcgtttg gattattttt taagatccta tcatcagtta
     2221 tacttaaaga aagaggaaaa atgtatgaaa atcattcgac aaattgaagt gtttgcacca
     2281 gattacttag gaaaaatgga tgtactgatt gccggtggga agattttggc tgtagaagaa
     2341 cagctagaag gcggctacga aggagtggaa gtagaagaac tttcaggtga gggaaaagta
     2401 ctaaccccgg gtttcattga ctgtcatttc catattttag gtggaggagg agaaggcggt
     2461 tatcaaaatc gaacgccaga agtcacattg agccaattga ctacagctgg tgtaacgaca
     2521 gttgtcggtt gtttgggaac agacggagaa ggtagagata tgactgcgtt gatcagcaaa
     2581 gcgaaaggtc tggaagcaga aggaatctcc acttatgtat acgaaggttc gtaccgattg
     2641 ccggtaaaaa cagtaacaga ttctatcatc aaagactttt tgacaattga taaaattatc
     2701 ggaatcggag aaatcgcagt atccgatcac cgttcttctc agccaagttt tgaagagttc
     2761 gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat ccggaaaagc tgggatcatc
     2821 aatgtccact taggcggagg aaaaagaaag ctagagctat tgacacgaat cgtagaagaa
     2881 acagaaattc cgatcacaca atttttgcct actcatgcaa atcgcacacc agaattattt
     2941 gaagcttgcg tggcatttgc caaacgtggt ggcacgattg acttcactgc tagcgaagat
     3001 cctgattttt gggagaagac agatggtgaa gttcgcttca gcaaagcact gaaacgattg
     3061 atcgaggaag atgtttcttt agataatttc acgatgacct cagatggaca aggaagcttg
     3121 ccttattttg atgaaaacaa tcatttccta gggttaggag taggaagtgc caaggcattg
     3181 ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc ctttggaaat cgccttacga
     3241 gctataacca gtaatccagc acgtatattg aaattggata aaaaaggaaa aatcgaaatt
     3301 ggtgctgatg cagatctgtg tatcttggat aaagaaacgc ttgatattga tacagttatc
     3361 gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag tatgggggac tttcgaaaaa
     3421 agtttttaat caacagctct aactatcttt tttagaaaac gagcgttctg tttgtctgta
     3481 tctgttgttg atgttaccct gaaggtagat caataaacaa actaagagga ggaagcacaa
     3541 tggcagattt aaaaggacgt tctaaagata tgaaagataa agtagtcggt gaaggaaaag
     3601 aagcatacgg caaagcaaca gacgacaaga gcaaagaaac agaagggaaa gcacaatcac
     3661 taggtgctga catcaaagaa aaagctcgaa atttaggcga cgaagtcaaa gacaagtttt
     3721 ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa ttgaatcaag caaataaaaa
     3781 atccgaacca tatctgcgag acagatatgg ttcggatttt ttatttgatc attatcctgt
     3841 tttttaaact tcgatttttt gaaatttttt attttttagt tcaaagactt catcacagct
     3901 ttttgctact tcaatctcat gagtcacgat gataatacag cgattttgtt catgggcgat
     3961 ttcttggaaa agtttgacga tatcttgtga agttgtttca tctagatttc cagtaggctc
     4021 atccgctacg attagttgat gttcacagca gatagcacga acgatagcga ctcgctgctg
     4081 ctgacctccc gaaagtttgg tcacctgttt ttttgctaat atttcatcaa ttcctacacg
     4141 agccagtcga tctaaagcaa cttgtcgata attctcttgc ttggtcttag tgatttccat
     4201 tgctgctatg acgttgtcta gagccgataa ataaggaagc aagttgtaag cttggaaaat
     4261 aatcgagaca tcatttttac gataacttct taacccgatt ttgttcagtg aacgatcttg
     4321 atagtatacc tttccttctt ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt
     4381 ttttccagaa ccacttgctc ctaaaatagc gtacattttt ccagcttcga aattcagttc
     4441 tacatcttta taaagcgcat cttgcggatc ttgataccaa aatcccaatt gttctgtccg
     4501 taacatcaaa ttcctccttt agtttaatag gatttttctt gggtttaatc ttaatatccc
     4561 tatagaagct aaaaagacag aaaacagact gattccaaat gctactccgg ctaatagtcc
     4621 caattgtgca ggtttgacat tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg
     4681 attattcgtg gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg
     4741 catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag
     4801 tagctgctgt cccacggcat tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc
     4861 aagaatcata caaatagcca cttcggtaaa caattggaga atgattttac ttctggattc
     4921 tcctaaagat agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa tcataatgat
     4981 cagagtaagt atgataatac cggctacagc cactagtaaa acaacatttt tagaaaaact
     5041 tgccacgttg tttaaaggtt ccaacattga ctgatacatg gaatcatttg attgtaagct
     5101 aaatgtatct gtgtcgatca gcttctcagc ttctttgaga aactcatcca ttttatctgg
     5161 atcagataga gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc catttagttc
     5221 atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat
     5281 acccatactg ccgcttgttt cgctggattc ataaattcct ttgatcgtca tttcataagt
     5341 ggtatcttca tcttcagtac tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt
     5401 ttctgctaga gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt catctattcc
     5461 ttcaccatcc gtgatttttg ctgttccatc agaaaaatta ctattttgtg cagttgctga
     5521 cacaccactg atttgaaagt ctgcttgagt catttgcgga ttcccgccca ttcctccagc
     5581 aggttcaccg cctgcatcag tagtttctga actgtcttca cttgaatcag aactagtgat
     5641 ggcagttatt ccatcttttg ccatagctga ggcggaactt tcaaaggaat aactctttac
     5701 attatctaat tcagctattt tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact
     5761 gcctggatct ggacgttctt cagtggtaga accgcttgaa gaagtttgtt cctgtttttt
     5821 aaatgaagct tctcgattgg cacttaaagt cactgttgct ccaacttctt tttgtgcttg
     5881 attcgctgct tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa aaataagaat
     5941 ggctgtgaac acggcaaaca ataacaatga acgtccccat tttacttttg tacttttcca
     6001 tgcacgtttc ataaaattca tattttgcat ccctcctagt agatgcattt gataatagac
     6061 aacctatctt aaagctagct taaatcttat gctgtttgca caccatcaac atgaggttct
     6121 gtatatataa aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt
     6181 ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag atgctacaga
     6241 gggattttta cgtgcatagt tgttctaaca attgctgata ctagcgattt agtttatttg
     6301 ttgtataata atggggaaca gctgaatact tatataagta ttctggctat agcgtttcgc
     6361 aaaagatcca ttttgttaat gaagtatatc caaaataaaa tgcccatcca actgtcagaa
     6421 cgcggatgtg ctggtaattt tcctgctgat tctccataag ttgagaaata gtgattttgc
     6481 atatgcttca ttggaatggc tcatatattt agataaagaa gaaaatcgac atctttaaac
     6541 ttggatgtca taagaagagg tgaagtttgt gagtaccatt aaaattatcc cattaggcgg
     6601 cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata tttttatttt
     6661 agattgtggg ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg taatccctga
     6721 ttttacgtat ttagaagaaa acattgatcg tgtagctggt gtctttttaa cacatggaca
     6781 tgcagatgcg atcggggcac ttccttactt gttgtcaaaa atttctgtac cagtattcgg
     6841 gacagagttg acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg
     6901 atttaaggat ttccatgtgg tagatgagca tacagaaata gactttggtg aaacagtagt
     6961 gagctttttc cgtaccactc atacgatacc agattcagtg gggatcaatc tgaaaacaaa
     7021 agaaggtagt atcgtttata cgggagattt taaatttgac caaacggcta tcccaatgta
     7081 tcaaacagat tatgcacgat tggcggaaat cggcaaagaa ggagtcttag ccttactaag
     7141 tgaatcggca aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa tagcagatga
     7201 agtatttgat acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa
     7261 tttgcaacga gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa aagttgtctt
     7321 gactggtcaa gatttcgaac gaattattcg cacagcgatg cgtttagaaa aacttcaatt
     7381 accaagtgaa gatctgcttg ttacgttgaa agacatgaaa aactatgatc cagaagaact
     7441 gatcatttta gaaactggcc gaatgggtga acccatcaag tctctgcaaa aaatggcaaa
     7501 cggtacacac cgtaatgtca agattgaaga aggagatttg gtttatatca ctactacgcc
     7561 aagcatcgcg atggaaacca tcgtggcgaa aacggaagat atcatttatc gagctggtgg
     7621 aacagtgaag ttgatctctg agaatatgcg cgtttctggc catgccaatc ctaatgactt
     7681 gcaattaatg atcaatttga tgaaaccaac atattttgtg ccagttcaag gagagtatcg
     7741 tgaattagct gctcatgcag atttggctca tgcagtaggg atgccataca aaaatatcta
     7801 tattactgga cgcggagacg tgctggaata taagaacaag cgtatgagtg ttgcaggaac
     7861 tacaagtgca gaaaatgtaa tgattgatgg attaggcata ggtgatatcg gaaacattgt
     7921 tttacgtgat cgaaaaatct tgtcagaaga cgggattttc gtagcggttg tcactatcag
     7981 tcggcgagaa aagaaaatca tttcaaaacc tcaaattact tcacggggat tcgtttacgt
     8041 aaaagctagc cgtgatctga tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa
     8101 acatttagaa agcaatgatt ttgagtggag caaattaaag caagacatcc gagatcaatt
     8161 aggccgttac ttatttgaac aaacaaaacg ccgcccagtg atcttgccag tcattatgga
     8221 agctactcaa cgtcgaaata aaaaataaac aaaaagagta agatgaacct ctctatagag
     8281 tgctcgtctt gctctttttt tgcgaaaaac aaataatttt tgaatacagc atccctcgct
     8341 ttataataaa cgatagaata aagggagtga atcaatgatg agtcaaacaa aagtcgaacg
     8401 ttatttagaa gaacaaaaga tcagctatca accacttgat tttagtcatg tggtagaaga
     8461 atctttcaca gaagaactac aaaaaagagg gattgatcca gctttgatat gtaaaactct
     8521 cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg attcctttga acgatcactt
     8581 agattacaaa aaaacgagac aagtgacgaa agatcgtaaa gttggttttc ctggaatgga
     8641 ttttgtatta atgcatacag gatatcccca cggagcaaac acccctattg gaataaaact
     8701 cgcacatcct gattattgtt ttttagcaga cagttcttta aaagaaaaag aagaagtaat
     8761 tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa gtagccgatt tagaaaagat
     8821 cgtccatcct atttacgctg atttaattag ttaagggacg agaagagaag ctaagcagag
     8881 cagagacctc aaacttgaga ttatatctgc ttggtacttg gtgcatcctg tattatctgt
     8941 tacaattgct ttgtacatac tgtactatca atccaatgaa aatatattta gcagctatat
     9001 tttttcattg atgtgcaagt gatgaaatcg tttttaataa aaaagataca gagaaaacgc
     9061 gaggtgtaaa atatgaaaat tacgtatcat ggacattcct gtttaacaat tgaaatggaa
     9121 aatggccaac gcgtgatgat tgacccgttt attacaggaa atccattgac agacttaact
     9181 gcagaagaag taaagacaga ttggttgctg attactcatg gtcatagtga ccacatagga
     9241 gatatgattc ctattgcaaa agaaaatcaa gccacaatta tcagtattgt tgagattgct
     9301 gattatgcga acagcagagg agtagattca ttcggtatga atatcggtgg aaaacatgcg
     9361 tttccattcg gcgtggtaaa attcgtacat gcacagcata gttcaagcta tgaagtagac
     9421 ggtatcgttc agtatatggg cgagcctagc ggaattatta ttcaagcaga aggaaaaacg
     9481 atttatcatg caggggatac tgcttatttc agtgatctag gattgcttgc agaagaattc
     9541 gatattgatg tcgctttctt gccgattggc gataactata cgatggggcc agaagatgca
     9601 gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc caatccatta caatactttc
     9661 cctgtcattg aacaagatcc cgaatctttt gtagagttac tggaagatag tgaaggaaaa
     9721 ataatgagcg tctcggaaac gctggaagtg taggcagaaa atggcgacaa aacatgattt
     9781 gattttagaa tatattgaaa gtttacctgt tggcaatcga atttctgtaa gaagcatcgc
     9841 aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca atcaaagatg cagaaaatgt
     9901 cggactagtt tcaacaattc aacgagtagg gaccatccgt atcgaacgca aattaaaaaa
     9961 acatattgaa cgtttgacat ttggcgaggt cgtcaggatc attgaagggg acgttttagg
    10021 cggggcggca ggattagaca aggtcttgaa taaattcgtc atcggcgcga tgactaaaca
    10081 tgccatggaa cgctatatta cgccaggatc actaatgatc gtcggcaacc gggcggaagt
    10141 ccaaaaatta gcattggaag atggcgccgc agtattgatc acaggaggat tcaatacgac
    10201 tgctgagatt gcaaagctag ctgatgatct tgaaatgccg atcttacgca cctcttatga
    10261 tacatttaca gtcgcaacca tgatcaaccg agcgttaagc gaccaattga tcaaaaaaga
    10321 tatcatgctt gtcagtgata tctatatgcc agctgaaaag actcattatt tgcagcagac
    10381 agatacgatt tctgattatc agcgtttgtc agaagaaacg cagcattcgc gttttccagt
    10441 cgtcaaccgc catcatcgtt tgatggggat tgttacggca aaagatgttt tagggaaatc
    10501 gccaaaccag atcatcgatc gggtgatgac gaaagaacca atcagtgtaa agaaaacaat
    10561 gagtattgcc tctgttagcc atcaaatgat ctgggatggg ctagaagtca tgccggtcgt
    10621 ttccgatgac ttgacattgg aaggcttgat tacccggcaa gatgtaatga aagcgatgca
    10681 gcttgtccag cgacaaccgc agattgcaga tacgatttcg gatcagatat ctggaaatat
    10741 ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa aaccctaagt tcaaattcgt
    10801 agtagcacca caaatggtca atggcgttgg aaccatttct tttggcgtac ttagcgaaat
    10861 catttctgat gtcgcacaaa aaacaatggt gatgaaccaa aaacgaaata tcttgattga
    10921 acaagtgaac ctacattatt tgcggctgat ccaattggaa agcgaattag acattcgtcc
    10981 acgtgtattg gaaatcggca gacgttccgc taaactggat atcgaagtgt ttattgaaaa
    11041 caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg gaacgttctt agaaagcagg
    11101 agtgaaatga cagtacagca agaaatagta gatacaataa aaaaatacga taaaatcatt
    11161 atccacaggc atttgcgccc tgatcctgat gcattaggat cacaagtagg attagccgaa
    11221 ctattacggg aaagtttccc agaaaaagaa atacgtcaag taggtgaaac gattgaaggg
    11281 cttcgctttt tagcagaaat gcaagaagtt gaagatgtct tctaccaagg agcgctagtc
    11341 atcgtgactg atacagctaa ttcacccaga atcagtgatc aaagatatca gttggcagaa
    11401 aaactgatca aaatcgacca tcatcccaat gatgaccctt atggagatct tgtgtgggta
    11461 aatacaaaag caagtagctg tagtgagatg attgcagaat ttgctttgat gtttcctgaa
    11521 tctttaaaga tgaacgttaa ggctgcacgg ttactttatg caggaatcgt cggagataca
    11581 gggcgctttt tgtatccctc cacgacgtcc cgtacattag agattgcttc gatattgaga
    11641 aattatccat ttgatgcaag tgctttgaac agagagattg aacaaatgcc gatgaaagtc
    11701 gctaaacttt ccggttatct ttatcaaaat atccaggtgg atgaaaatgg tgcaggaaga
    11761 ataatccttc cgcaagaaat tcttgataaa tatggaatcg atgattcaga gacagcttcg
    11821 atcgtttcac ttcctggagt gattgacgaa gtgctggctt gggggatttt tgttcagcag
    11881 ccttcagggt attaccgcgt acgtttacgt tctaaggggc cgatcatcaa tgaattggct
    11941 aaacgccatc atggaggagg ccatccatta gcaagtggtg cgaatgcaaa agatttagca
    12001 gaagtagagg aaatctataa agagatccaa gatatatgcc aatcgtataa gagcagaaaa
    12061 acagaatgat acttcgttcg aaaacgtgcc ttttgtagta aaaatcacaa atgtatcttt
    12121 tcaagatctt ctttttccgt tacaatagtg ctcactaaag attttaaagg agaaagaaga
    12181 tgacattacc aaattgccca gaatgtggct cagagtacac atatgaagat cgaggcttgt
    12241 tgatttgccc agaatgcggg aacgaatgga acaaagaaga acaagaaact gtcatagaag
    12301 acggattagt ggtattagat gcaaatggaa accgtctgtt agagggcgat agcgtgacag
    12361 tgattaaaga cttgaaagta aaaggtgcgt caggtgccat taaacaagga acaaaagtca
    12421 agaacatccg tttagtagaa ggcgatcata atatcgattg caaggtagaa ggatttggac
    12481 cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag aatattcaag gtcttttcac
    12541 attcttttcc tactactaat gataaaataa agtaaacgaa aaggagtgaa acgaaaatgt
    12601 atggttatgg aatgtatggt tttttagatc cgacatttct tttggtcatt cttggattag
    12661 ttatttccgg aattgcgtca gcgtacgtca atagtacttt ccgaaaatac gatcaagtga
    12721 gaagcaaaaa caatgtgact ggtacacaag cagcacaata tatattacaa agtcaacaga
    12781 taaataatgt cggtgttcaa caaattgccg gagatctcac agataactac aattcaggta
    12841 ataagatgtt gagtttgtct caagcaactg cacagtctac ttcagttgct gcgataggtg
    12901 tagcagctca tgaatgcgga catgcggtac aagatgcttc gggttatgtc ccattgaaga
    12961 ttcgtgcagc acttgttcca gcagctaact ttggttcgat gatttcgttc cccttgatta
    13021 tggtaggtgt attgcttagc tggaactcta cgttaatcaa tatcgggatt ttcgcttttt
    13081 ctttagcatt acttttccaa ttggtgacat taccagtcga atttaacgcg tctcggcgag
    13141 caatccaaat tttgagtgaa ggcggactac tgacggaaga agaagtaccg atggcaagac
    13201 acgtattatt tgcagcagca ctgacttatg ttgcagcagc gctttcgaca ttcctgcagc
    13261 tgttgcgctt gatcttattg ttcggcggca accgaagaga ttaaaataaa gaagttgtga
    13321 aatcagcgct ttgatctgag ctaataccga gagttagctg attgaatacc atttatttgg
    13381 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
    13441 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
    13501 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
    13561 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
    13621 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
    13681 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
    13741 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
    13801 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
    13861 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
    13921 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
    13981 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
    14041 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
    14101 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
    14161 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
    14221 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
    14281 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
    14341 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
    14401 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
    14461 atccgtaaat ggaattttat taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
    14521 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
    14581 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
    14641 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
    14701 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
    14761 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
    14821 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
    14881 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
    14941 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
    15001 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
    15061 agatattctt gtttttaagg cggaaaaatt gatttttaat ggatataact aaatatgttt
    15121 tgataattat caataatatt tttagaatta tttatgttat ttatttatgg gtagatgaaa
    15181 agagacggag gtaagaatga tggggagagt aacaaattat agtaaagaat atttgctgtt
    15241 caaaagtatg gtatacgtgg aagaatatgg aatgaagtca attactgcaa gagagttagc
    15301 agagttctgc ggttgttcta catacccgat ttatacacat ttcaaatcaa tcagcgggtt
    15361 aaaagagaag atattagaag aaatcactgt ttattttgaa aactacttag ctgaatgcag
    15421 ttccgatgat gttttaagca ttgtctactt attgaaagat tttttttcta tccatgaaag
    15481 tattcgtgaa atcattgcaa aatctgattt agacatggat tcgctattta agcggccatt
    15541 tttctcatat gtcaaaggtc acacgaagat caaagaagaa aagtcgcttg aattggtatg
    15601 gcttaatgca ttgggaagtt tgcgctcaga tgattcttct caagattttc ttgaaatgat
    15661 gaacgctgtt tgggataaaa acgaggtgac ggatgaatac ttggaaaata tcttaaagga
    15721 acagtcacct taggcagcaa catgaaaaag tagatcagcc acctttattt ttcgtaaaaa
    15781 agattgtaaa atgtctaacg aaacattgga atgaaggagg aagcgacaac gttaccacag
    15841 caatttatca aaaaatatcg tcttctttta ggcgaagaag catccgattt tttttctgcg
    15901 cttgaacaag gaagcgtgaa aaaaggattt cggtggaatc cactaaaacc ggcaggattg
    15961 gacatggtcc aaacttacca tagcgaagaa ttacaacccg ctccctacag caatgaggga
    16021 tttcttggca ctgtcaatgg aaagtctttc ctccatcaag ctgggtatga atatagccaa
    16081 gaaccaagtg caatgatcgt tggaacagct gctgcagcaa aacccggtga aaaagttttg
    16141 gatttgtgtg cggctcccgg tggtaaatca acccaattag ctgctcaaat gaaggggaaa
    16201 gggttattag tgacgaatga gatctttccg aaacgtgcga agattttatc agaaaatata
    16261 gaacgatggg gagtttcgaa cgcaatagtg acaaatcatg cacccgcaga acttgtccct
    16321 catttttcgg gcttttttga tcggatcgtg gttgatgctc cttgttcagg agaaggaatg
    16381 ttccgaaaag atccaaatgc aataaaagag tggacagaag aatctccgct atactgtcaa
    16441 aaacgtcagc aagagatttt gtcttcagca ataaaaatgc taaaaaataa aggacaactg
    16501 atttattcga cttgtacgtt tgcaccagaa gaaaatgaag agatcattag ctggcttgta
    16561 gaaaattatc cagtaacgat tgaggagatt cctttgactc aatccgttag ttcaggacgt
    16621 tctgaatggg gaagcgtagc tggtttggaa aaaacaatac gtatatggcc tcataaagat
    16681 cagggtgaag gacattttgt tgcaaagctg acttttcatg gacaaaatca gatgcataaa
    16741 gaaaagaaga cacgaaaaaa aagtaaagtg caaatgacaa aagagcagga aaaactctgg
    16801 acggagtttt caaatgattt tcattatgaa gctactggaa gacttttagt gttcaacgac
    16861 catctttggg aagttcctga gttagcccct tctctcgatg gtttaaaagt cgtacgtaca
    16921 ggtttgcatt taggagattt caaaaagaat cgatttgaac ctagctatgc attagcgctt
    16981 gctacaaaaa agatagagaa tataccttgc ttgccgatca ctcaaaagga atggcaatct
    17041 tacacagcag gagaaacatt tcaacgtgat gggaatcaag gatgggtttt gctagtatta
    17101 gataagatac ctgtcggatt tgggaaacaa gtaaaaggaa cagtaaagaa ctttttccct
    17161 aaaggattga gatttcactg agtggattag ttcgtttatt tttttgtcta tgctatgatg
    17221 gagatagata aaaaggagct gatacttatg aaaacatcaa caaaagtaac aatcggatta
    17281 agcgtagcag ccgctgccag tgtagcgaca gcagtcgttg tatcgggaaa agtaatggaa
    17341 aagatccatc atatgactac tcggacgaaa gtaaaaaaat tcgtctatga caaatttgat
    17401 ggaaatgaaa aattactaga aatcgtagat ggtctttcag atagtgactt ggattctttg
    17461 atgggaatgt tagcaaaaat caaatcagga aaaaagaaaa tctctgttta tggtgattca
    17521 atcaaagatt caaccgaaga tgtaaaagac cggctaatgc attttgttga aaaaatgatg
    17581 taagagaaac tagcttttga acactattca ttcgaaataa ggggttgtga caaaattatt
    17641 gtcacaaccc ctttaatcta tctttaatta tgttcatgaa gcaataaaag atagcaaaac
    17701 agtaaatcgt tggctttttt tagattgaaa ccagactgtt cttgaaattt ttcgattcga
    17761 tattttagag tattacgatg aagaaacagt gtttttgcag cagagctgac attgcctaat
    17821 tcattccata attcttgaat gatcacttcc atttcatttt gtttgatcag ttctatgtaa
    17881 cttttgatca acttactatc ttttacagta tcttttgtat agtaatgtaa agccacatct
    17941 tgcatagaaa aagtacggct gtgagtataa agattgtttt gctctgttaa aaaaattttt
    18001 ctttcttcag caaacaaagg gacgaggtcg gtgccaggtg aatggaaaga accaacgaat
    18061 acagacgttg tcgtatcaaa atcagcatct aaagatagaa aaataccgtt gatttcagta
    18121 gcatcaagat aatcacggtt tatttgttcg actaagagcg catctgtgtc tgtataaaaa
    18181 aagaaatcag cagaatagcg aaataattcc aaaatagtat cttgccattc tttttttaag
    18241 aattctcctt ttgattcgac gtgaagctgg atcaaacgaa agcttttttc ttgaacacaa
    18301 gaagcatctt taaaaagata gtgataccat gggtgttttt tttgatcgtt gattacaggg
    18361 aataacgctt tcaaaaggct ggtttcttgt tgagataatg aatcttgttt gatccagata
    18421 aaagcatcat caattgcaaa agaaaggata tccgcttctg atgctggaga gttttttttc
    18481 tgtgcttgtg ggtaaagagc aagtaattta tccattttca tcttgttcac ctcttgtatt
    18541 tattttacca tagaaaaaaa agttagttac agccttttta tcatatgcta aagtaaaaca
    18601 agaaaaaaca aaggcaggga agggtaattg gtaaatacaa atcttgatgc agtagaaaaa
    18661 gaaaaatgga tgcgccttgc attggcagaa gcaaagaaag cagaaatgct tcatgaagtt
    18721 ccgattggag cagtggttgt attggatgga aaagtgattg gaagaggata taaccttaga
    18781 gaaaccacgc aagatgctac tacacatgca gaaatgcttg caatcaagga agcctgtgaa
    18841 aaagtgggaa gctggcgctt ggaagatgct gcactttttg ttaccttaga gccttgtcct
    18901 atgtgcagcg gcgcgatgat tttgtctcgt gtagcagaag tttatttcgg cgcgtatgat
    18961 ccaaaaggcg gaacagccgg taccttgatg aatcttttgg aggacgagcg gtttaaccac
    19021 caagcatatg tagaaggtgg cattttagaa gacgaatgtg ggatgatttt gactgacttt
    19081 ttccgaaagc tccgccagcg aaaaaagaaa ttaaaaaaag actggtcaac gaatgaaaaa
    19141 taaggtatac tattctttgc cgaaaggcta ggacaatggt gggcttgtga agcgtgtcag
    19201 gtctggaagg aagcagcact aagcaggttc cgccatgtgt ctgattataa tgaaacgaca
    19261 acagactttc gggtctgttt tttttatttt tatagtactg taaaaatgaa tgttcctgat
    19321 tttttagcca gactgattta tcgaatattg tacttataca atttgcttca aggcaggaaa
    19381 aactctgaaa tagagcttgt ttcaatggaa gaaaaagctg cctatggtat gataaagata
    19441 aagagaggcg agagggtaca tggaaaaaat agtgatttta catacgaatg atctgcattc
    19501 tcatttagaa aattggccaa ggatcagaag gtttttggat caacgtaaac gagaaaacga
    19561 aaagaaagaa aaaacgacaa cgattgcagt agatctaggt gattttgtgg atcgttggca
    19621 tccattgtca gaagcaacga atggacagga aaatatcttg ctgatgaatg aagttggtta
    19681 tgatgcagcg acgataggaa acaatgaagg cgtaggaaat tccaaagaag agttgaatca
    19741 tttatacgat catgctgagt ttgatatcat cctggataac ttattcgata aaaatacgct
    19801 ccagccaccg gcatggacga aaccatataa aatcattaca acgaaacacc agacgaaaat
    19861 cggactaatt gcatttactg ctccatttcc gcttacttat aatccgaatg gctgggacat
    19921 acggaatcct tatgatattt tgccagaatt agtcgaagat ttacgttcac aagtggatgt
    19981 tttagttttg atgagtcatc tcgggattca ggatgatttc caaattgcaa aagaaatacc
    20041 agcaattgat gttattttag gatcgcacac ccatcattta ttcagagaag gtcacgtaat
    20101 caatgatgtc caaatagcag ccgctggaaa gtacgggcag tatgtcggtg aagtccattt
    20161 aaccatcgat gaagagaaaa aaatccttaa gcattcagcc aaagccatcc ctaccgaaac
    20221 aatgacagct tttacagaag atgaacaaga aataaatagt tatctagaac gtgggcatga
    20281 attattaaaa gaaaaagaag tagcagattt gccattttct ctgagtctag atatcttcaa
    20341 tgagcattcg tttatccaag tagcctttga ggcggtgaaa gaacgaggaa agacggaagc
    20401 agcaatatta aacagtggtc tgtttttaac tgaaattcca aaagggcggg tcaatcagga
    20461 tcagcttcac actgcgttgc cgcatccgat gcacttgtta aatgtcacat tgggtggaaa
    20521 tgatctgatc cgcttagttt tagaaataga aaaaaatcgt aactttttac gtaactatcc
    20581 tatgaaggga atgggatttc gcggtaagat ttttgggcag atggtctata atggaatcac
    20641 atatgattct attaaccatc aagtcttttg gaagaacaag ccaatcgatc tgaataaaaa
    20701 ttatactttt actaccgtag atcatttgat gtttgttcca ttttttccaa cgattgagat
    20761 tgctggaaaa aatgaatttt tgttccctga atttatccga agtgtggtag gagattactt
    20821 gaaagctcac tacccaatca aataaaaaca aggtataata aaaaagatag gtggtgaacg
    20881 catgtctgca aaacaaaatg aacagcagaa cgttgagcat aaaacagaaa ttgctaagaa
    20941 aaaatcatca gctgaacagg aaacaacagt caccgaagaa ctgacagctg ctttggaaga
    21001 aatcgttgag gagcctaaag cagaaaaagt caaaggcgag ctagtgacac tgttagatga
    21061 cagtaatttt ttgataggtg aaagacatta tcggcttgtg tccgattatc gtgaaggatt
    21121 taatgcagaa aagcttggag aacgttacag tgatgtacta gcccgatatg attatatcgt
    21181 aggagattgg ggttatgaac aactacgcct aaaaggattt ttccaagcgg ataatcgacg
    21241 ggcgcatcca gatcagcgaa tcgattcctt ggaagactat ctttacgaat attgcaattt
    21301 tggttgtgcg tattttgtca tcgagagaat cggcggtaaa aaagaaaaga caactcagcg
    21361 ccgaaaaaag aaaaaaaacc ataatcgtgc gtatatcgat gagaaaaaag gaccagtcgc
    21421 atctaatcgc aaaaaaccgg ttattaaaaa tcgaaaaaca gagtcagcaa aaacctctga
    21481 aaaagtaaaa aaacaagaac aacctaaaca agtagaaaaa acaaaaacag catttacgat
    21541 cagaaaaagg gaagagtagt ggatgaatgg actataaagg atatttaatt gatttagacg
    21601 gaacgattta tcgtgggaca caacccatac cggccggtaa acgatttgtg gaagaattgc
    21661 aaaaaagaaa attacctttc ttgtttgtta ccaataacac gactaagtca ccagaaacag
    21721 tagctaatcg tctagcagat gagtttgaca ttcatgtagc tccagagaca gtctacacag
    21781 ctactttggc aacaatcgat tttatgaaag cagatggaaa agggaaaaaa gtctatgtta
    21841 tcggagaagc aggattgatt gacttgattt tagctgccgg atttacatgg gaagaagagg
    21901 ctcctgacta tgtagtggta ggcttagata attatctgac ctatgaaaaa gtagttaaag
    21961 caacacttgc tattcaaaaa ggtgctactt tcattggaac gaacccagat aagaatatac
    22021 ctactgaacg cggccttctt cctggagcag ggtcagtcat ctcttttgta gagacagcaa
    22081 cccaaacgcc gccgatctac atcggaaaac cagaagccat cattatggat aaagccgttg
    22141 aagtattagg gctgcaaaaa gaagaagtaa tcatggttgg agataactat gaaacagata
    22201 tccaagctgg gatacgcaat aatatagata ctttgctcgt tttatcaggt tttacaaaag
    22261 aagaagatgt acctgggttg cctatcccag ctacctatgt gaaacaatca ctggatgaat
    22321 ggagcttcta atatgaaaaa ttcgagatgg cagtggatgg aatatgctgg attgttcagc
    22381 ctatttctga cactgatcag tctggcagtt ggtgtgacga tcaactttcg accactatac
    22441 gtatttgata tcggacattt gcaaattttg gattatactt ctttagatca agagacgtta
    22501 ctgaaaaatt ttgatcacct aatgaattat ctcaataatc ccttcaaaac catcctcagc
    22561 ttgccagatt tcccagtttc tgctagcgga gcccatcact tttatgaagt gaaaattttg
    22621 tttttagtgg attatgctgt gtttttcatc actttgatcc cgagtatttt gtttatcaaa
    22681 tatttgcaaa agaatgatcg gctttggcgg ttgattcgtc cgtttcaaat cggcatgtta
    22741 ctgccagttg ttttcggttt tttcatgatg ataggatttg atcgcttttt tatccttttt
    22801 catgaaacgt ttttcaacaa cgatgactgg ttattcgatc ccgttactga tccaatcatc
    22861 aatgtacttc cagaagaatt tttcatgcat agctttatct tattttttgt attattagaa
    22921 ttattttttg ctgtttttct tttcttagga aaaaatagct tgaaacaaac aaaaaagaaa
    22981 gaactcgtct aaatagacgt tttctttctt tttttgttga attattgagt aaggcgactt
    23041 cttttttctg gttctttgtg tgctaatcga ctggcagcag cagcggcgat cgctccgacg
    23101 atatcatcta agaaggtatg gacatgaacg ccatcgtggc tgtttagttc ttttaaaata
    23161 ccaggtttca ccttgtcgat ataaccataa ttcgtaaagc cgatcgtacc gtaaacattc
    23221 acgattgaca aggctaggat ctcatcgata ccgtaaaggc cttcgtcatc ttcgataata
    23281 tgttgcaacg gactcatcaa ttttttcttt tctgctaata tatctaattg gattccagtg
    23341 ataatggcgt tatgtacttc acgctttgtt agcacgctgt tgacactttc agtacaatcc
    23401 gccaaagtca aatcagaaat atagtctttt tgtaaaaaca tgacaagttg tgcgatatct
    23461 tctacagtta cgccacgttc cttcaatagt tctcttgcct tttcttctaa agtctcagtt
    23521 ttgacgacca tattattgct ccccctttat tttttctttc tctatttcat gttaacggaa
    23581 catgaaaaaa atgcaagaaa ctgctttcgg gtttaacttg aaatgcaaag agatataaaa
    23641 aatcaactag gacgaagatt tttatcgtat aagcttgtgc tgtgaccagg ctgagttcgt
    23701 tcctttggat taatatagtg taaagcgttg ttcactgcag taggtgcttc accgagccca
    23761 gtagcaatca gtttcacttt tccttcatag gtacagatgt caccagctgc ataaacgcct
    23821 ggaatagaag tagacatatc tgattttaca gtaatggcat ttctggtact atcaagtccc
    23881 caagaagaaa gatgttctaa attagaagag aatccataat tgacgatcag agaatcaatc
    23941 atcaaatcaa tcgtctcgtc acttttcact ttttttaacc ggatatctgt cagttcccca
    24001 ttattgcctg ataatccatc gatgagatag ggagtaagca agttgacaga tgaagatttc
    24061 aagcgtgata cactatgttc gtgcgcacga aattccgggc gacgatgaat caagtaaact
    24121 tcgcttgcaa taggctctaa catcaacgtc caatcgatag cagaatcacc acctccagca
    24181 atagcgactt tttttccagc atacttcatc aaatcattca cgaaataatc caatccgtgg
    24241 ttttcaaatg attcagcatt atctaaattc aattttcttg gctggaatga accatttcct
    24301 aaagctaaaa tgactgcttt ggaataatgg atgcctttat ttgtagtaat ctcgataact
    24361 tcgtcttcac gagtaagcga gagaacttct tcttttaaat ggaatgtatg gttaaaagta
    24421 gttaattgtt tttctaattg atcgattaat tcacttgctt ttattgctgg atacccagga
    24481 atatcgtaaa tatatttttc gggatacaaa gttgctaatt gaccaccaag ttgaggtaga
    24541 ctgtcaatga tttttgtttt agcttggcgc attcctgcgt aaaaagcggc aaacatccct
    24601 acaggacctg caccgatgat tgtaatgtca tagatttcca taaaaattcc tcccttttca
    24661 cactatatca gaatttaatt gagaatgctt ctcaaaaatg tttgaattcg aaatcgaaac
    24721 ggtaagttat agaatgtatc tagtgatgaa aaataaaaga aaacaaatct agtattttta
    24781 cgagaggtta ggtattatgt tattattatt atgaagtatg caaatattag gaggaaacaa
    24841 tatggcattc ccacaactag atttaaaaag tgaaaaagga ccaaaagctg ttatcaaaac
    24901 aaaccgtggt gacattactg ttcaattatt ccctgaacta gcaccgaaga cggttcaaaa
    24961 tttcattgaa ctttccaaaa aaggttatta tgatggcgtg atttttcacc gcgttattcc
    25021 tgatttcatg atccaaggcg gcgatccaac tggaacagga atgggtggcg aaagtattta
    25081 tggcgaaagt tttgaagatg aattcagtcg tgaactgttc aaccttcgcg gagctttatc
    25141 tatggcaaat tcaggtccaa acacaaacgg aagccaattc tttattgtaa ataacacaaa
    25201 tgtgccagct aatatgcttg gacaactaga aggtgctgga ttcccatcag aaatcattga
    25261 agcgtataaa ggtggcggaa ctccttggct agatttccga catacagtct ttggacatgt
    25321 actagaaggg atggacacag tagatgaaat cgcaaatgta caacgtggtc cacaagaccg
    25381 acctgtacat gatgtagtga ttgaaacaat tgaaatttca gaatagtttg gtatggtacg
    25441 gagtaattat gaaaaaggac gaaacctagt aaggtctcgt ccttttattt tgaataaatg
    25501 atttaatcga ttgtctctaa agcatacttg attcgattaa gtccttccag taaagtttct
    25561 tctggacaag caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa cgtgatgcca
    25621 ggatttaata cgacttttcc tttccgaaca agagtgttct ccaattcttg gtcgtctgaa
    25681 atataggcgg taaaatctaa ccacattaag taagttcctt cagggttcat tattctcact
    25741 ttcggaagat actgttcaaa aaactgatta actgtttgaa catttttttc tagataagga
    25801 atcagttggc ttaaccattc tccacccgtc tcataagccg cttgggttcc aatcagtcca
    25861 aaagtgttga tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc agctttcatc
    25921 tctggatttt tgataaatac cattgaattt ttgattgcag ctagattgaa agtcttggtt
    25981 gcggaagtaa aagcaactag tagatcatct aaattaggat caatcgttag catggaagta
    26041 aatgtatgat cgaataaagt taaatcttga tggatctcgt cactaaacag aatcacttga
    26101 tgttttaaac acaattcgct taactgtttc aattcttctt tgctccaaac acgccctcca
    26161 ggattgtgag gattgcataa aatcattgct tttacattgt tttcttcgat ttttttctcc
    26221 atgtcagaca agtccatgat gaaatgatta tttttttcta gcaaagagct tcgaacgatg
    26281 tgacgttgat ttgtttctat aatagaggaa aatggaggat aaactgggtc atgaatcaaa
    26341 atagaatcgc ctggttttgt aaaagtttgt actgctgcag ctaaactggt taacacacca
    26401 cttgtaaaaa cgatattttc tttcgtcaac tccacagcat ggtgttggcg ttcccaatga
    26461 atgatagcct gatatagttt atcagacaga gtggcataac caaagattcc atgtttgatg
    26521 tattcactaa acgctttagc taccccaccg ggagacaaga agtccatatc tgctacccac
    26581 aaaggaagta agtcatccat ttgatatgtc tcagcaattg cgtcccattt tacactttct
    26641 gtatcttttc ttgaaatctg tttatcaaat tcgatcatct taagacctcc agtcattttc
    26701 gtcgcttctt tttttgagct atttacagta taatgataaa tgcgaagaga gggaagtaga
    26761 tgacttataa aattggacaa ataattgaag gaaaagtgac agggatacag ccttatggcg
    26821 cattcgtttc tcttgataac gagacgcaag gacttattca tgtttcagaa gtacaatccg
    26881 gttatacgaa aaatatccat tcattgttaa aagtcggaca gccagtaaca gttcagatca
    26941 tcgacattga tgaatattcc aaaaaaatca gtttatcttt aagaacgtta gaaaaaacaa
    27001 atccagctgt tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt aagatcggat
    27061 ttaccactat tgcggagcag ttacctattt ggatagaaga agcattatct gaactagaac
    27121 ggaaagaaaa taagaaataa aaagtgtatt ttttctgaaa atgtgctaga atttttatgt
    27181 aagccataga acaagttgat cgaggtggaa attttggcaa gcaaaagagt tgctggtaca
    27241 attatgttgc atttggagga tggtactaaa aaatttttgg tccattcaat tgatgacaag
    27301 ttggaatttg cgttagcgga actttctgaa ggaaagactg gtttagcgaa tatacttaat
    27361 ttcttaaaag aaatagttca tatcgatgtt agcaagatta gtttgatgga attgacaaat
    27421 acacatatta aagaggaaaa tgttccgttg tttgtctttg aaactgaaca aaagaatcaa
    27481 agagaagaat taggcgaagg atatatttgg gaagaaccag gtcaaatgcg ctctgttctt
    27541 ggaacttacg aagtagaggg agtgcctttc ttctaaaata cagattaagc gaacaaagaa
    27601 gtttgaattt ttttcaaact tcttttttta ttgtttggtc caaaaaatac aatgaaatgt
    27661 tctgctaaag atagtttttt agtgcatttt attttcaaaa tatgttgaaa acggttgtat
    27721 aacgaataat aataattgat cgaaaaatca tttcacaaag taatgcttaa tttatagttg
    27781 accaaggaag aaatacatga taaatttaac agcgttgatt ctgaagtgga tgttatatca
    27841 catttctaaa aaatttaaaa aa
//
LOCUS       DAZUKT010000031        27691 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000031 DAZUKT010000000
VERSION     DAZUKT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.031"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..665)
                     /locus_tag="ACG816_001355"
     CDS             complement(138..665)
                     /locus_tag="ACG816_001355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4920238.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(732..2234)
                     /gene="eat(A)"
                     /locus_tag="ACG816_001356"
     CDS             complement(732..2234)
                     /gene="eat(A)"
                     /locus_tag="ACG816_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4920239.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2492..3613)
                     /locus_tag="ACG816_001357"
     CDS             complement(2492..3613)
                     /locus_tag="ACG816_001357"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4920240.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3739..4680
                     /locus_tag="ACG816_001358"
     CDS             3739..4680
                     /locus_tag="ACG816_001358"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4920241.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4830..5726)
                     /locus_tag="ACG816_001359"
     CDS             complement(4830..5726)
                     /locus_tag="ACG816_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4920242.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5995..7335
                     /locus_tag="ACG816_001360"
     CDS             5995..7335
                     /locus_tag="ACG816_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4920243.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7553..7996
                     /locus_tag="ACG816_001361"
     CDS             7553..7996
                     /locus_tag="ACG816_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4920244.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8500..9918)
                     /gene="nhaC"
                     /locus_tag="ACG816_001362"
     CDS             complement(8500..9918)
                     /gene="nhaC"
                     /locus_tag="ACG816_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4920245.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10321..11544)
                     /locus_tag="ACG816_001363"
     CDS             complement(10321..11544)
                     /locus_tag="ACG816_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4920246.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11575..12414)
                     /locus_tag="ACG816_001364"
     CDS             complement(11575..12414)
                     /locus_tag="ACG816_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4920247.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12431..13111)
                     /locus_tag="ACG816_001365"
     CDS             complement(12431..13111)
                     /locus_tag="ACG816_001365"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4920248.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13113..14186)
                     /locus_tag="ACG816_001366"
     CDS             complement(13113..14186)
                     /locus_tag="ACG816_001366"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4920249.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14242..14480)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14523..14756)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14833..15504)
                     /locus_tag="ACG816_001367"
     CDS             complement(14833..15504)
                     /locus_tag="ACG816_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4920250.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15591..18239)
                     /gene="mgtA"
                     /locus_tag="ACG816_001368"
     CDS             complement(15591..18239)
                     /gene="mgtA"
                     /locus_tag="ACG816_001368"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4920251.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(18581..18755)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18928..19365
                     /gene="ndk"
                     /locus_tag="ACG816_001369"
     CDS             18928..19365
                     /gene="ndk"
                     /locus_tag="ACG816_001369"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4920252.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19387..20253)
                     /locus_tag="ACG816_001370"
     CDS             complement(19387..20253)
                     /locus_tag="ACG816_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4920253.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20413..22506)
                     /locus_tag="ACG816_001371"
     CDS             complement(20413..22506)
                     /locus_tag="ACG816_001371"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4920254.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23255..23878)
                     /locus_tag="ACG816_001372"
     CDS             complement(23255..23878)
                     /locus_tag="ACG816_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920255.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(24037..25434)
                     /locus_tag="ACG816_001373"
     CDS             complement(24037..25434)
                     /locus_tag="ACG816_001373"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4920256.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25466..26437)
                     /locus_tag="ACG816_001374"
     CDS             complement(25466..26437)
                     /locus_tag="ACG816_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4920257.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26811..27236
                     /locus_tag="ACG816_001375"
     CDS             26811..27236
                     /locus_tag="ACG816_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920258.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         7977 a         5730 c         4814 g         9170 t
ORIGIN      
        1 acttttatta aaataattaa atttcatttc taaaggttga gtttatatta tcaaaagaat
       61 tgtcataacc taaaaaaggc actgctaagc aaaagcagtg ccttgaaata ttttatttaa
      121 ataacacaaa aacataatta ctcataagaa accaattctg tattatctac catgaatgta
      181 aagaacccct cattaggcat cggatgaaaa taagcattga tcactcttat caaggcatta
      241 tggcaaacta acagatagac attttcttcg tcttcgatgc attctttcaa taaaggaacg
      301 atccgtgcat acacatctaa gactgactcc ccatttggga atcgtacagc gaacgctaaa
      361 cgtgcttttt gaaagttctc atctttgctt ggcagtccat cataatcccc aaaatccatt
      421 tctaccaatc gttcatcaac ggtcagcggc aagctgagac gatcagcaac tgcttgtgct
      481 gtatctcttg ctcgttgaag aggggaatgg atgattttcg tgattggaac aggcaaatct
      541 actacctttt ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag cggcacatct
      601 gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct cgttacataa
      661 agcatgtgat tctctccttc gttggcaaag ttccccttaa ccataccttg ttgttcttga
      721 gattacaagg gttagattct ctcaagagaa ataatctcag tagatacttt atccaggaag
      781 gtttgatcat gttccactag taacatggca ggtttcacgt tcaagatcag ttgttctaat
      841 tgttcttgat tgaagacatc caaataattc aatggttcat cccatgtata tagttcagct
      901 ggctgtgata aagatttagc caattccact tttttccgtt ggcccatact catctgctcg
      961 atcttgttat gaaaaacatc tctttccatc ccaagctttc ggaggttgtt caaaaatgct
     1021 tggtagtcta cttggttttt ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa
     1081 ttttggcttg cataactaat gctcagatgt tttggctggt atttttctcc tataaccttg
     1141 ccgttgaatg cccccagaag ataatggata atggatgact ttcctgcacc gttaggacct
     1201 gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa taacagattt
     1261 tcatacccta attgaagatc ttctacgctt aaaagtcgct tatggtgaga cgcttggcta
     1321 ttcatcgtca aagaatcgat atattcgata tcttttaata gtttttcttt ctcgctgatc
     1381 tgggtctcca tccgattcac gattgctttg gatcgtttca tcgtccgtgc agcatcagca
     1441 cccactgctc ctttattcac tcgtctagat tcagtatcga tgaatccgac ttgtttcttt
     1501 gttttatctc cttctcggga acgagaccat tcggcttttt cagctgctgt tttctttagc
     1561 ctactgactt cttttttcaa tttttcattt tgagccattt caaattcatc acgaagtttt
     1621 ttctgttctt catagataga gaaattccct tgataaagtt ccagttgact tttttcgatt
     1681 gctaaaacat ggtccactac ttcatcgata aatccccggt catggctgac cacgatgaag
     1741 ccttgttttt tctttttcaa ataagccgct acttgttttc taccagagat atccaaatga
     1801 ttcgttggtt catcgattaa cgggaaatga gtgtcatcca caaataaaag tgccagtagg
     1861 acttttgtct tctctccccc cgatagtgtg ctgaattctc tccataagat ttcaggatct
     1921 gtttgcatca attggagctc tctttcgatt tcccatatct caaaatccgt aatatcattt
     1981 aacacgtaat aggttaaacg ttctttatct tttgtctgtt gcgggaaata ggcaaattct
     2041 tgctgatgga ttacttgccc ttgataaggt agtttgtttt gtagaatatt cagtaaagtt
     2101 gtctttcctc gaccgtttcg tccgataagt cctagtttcc attgtgtgtc aaaatttaga
     2161 tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt
     2221 tcgattttag acattttcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg
     2281 ctaaagagga tgcaagggtt ggatacacac aaaaagaccc atacatcgaa aaaaccaagc
     2341 caatcccgaa aatctgcaca ctgcttcctt gtctccacaa ggtacagtct gaatgcagct
     2401 ttcatcaatc gacttaattt ttcaatgcac ggatccctca ctttgtccta tatttgaaat
     2461 tacgatagca taaagaaaat gattttgtca actacttcgg gaggatggtg aaaaatctgc
     2521 taagtgtctg tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc attgcttctt
     2581 ctaaacctat tggacccaaa tgaatactta agacaattgg gagaaaatca gaaagtcctt
     2641 ctagctcttg gtctctactt ccgcaaatag cgattattgg ttttcgatat ttttttgcaa
     2701 gcactgctac accgtatgga acttttccgt ggatcgtttg ctgatcgatc cgcccttctc
     2761 ctgtaatgat gacatctgct tgttcgatct ttttttccaa tccagtgatt tggctgacta
     2821 aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag ggctccacca ataccgcctg
     2881 ctgctccact tcctggaata tcatctaaat ccacctccca gaaatctttt atttgctgag
     2941 caatctttcc tgcttgttta tctaagaaag ctagcgtcgt atctattcct cctttttgtc
     3001 ttccaaattg ccaagcagct ccttgttgac ctgtatatgg attggtaaca tctgcagcga
     3061 taatcaattt tactttagaa acaagaacgt ttcttcgatc aaaatgaagt tttttagtag
     3121 ataataaagg atttccttct gtgtaccctt ctaaagaagc acccaaggat tggagtaatc
     3181 ctagaccacc atcgctacac ccgcttcctc ctaaagtgac aataatttgt tctacttctt
     3241 gaagcagtgc atctttaata agttctccta aggcataact ggaagcaagt cggatagttt
     3301 gatccgttgg tttatcaagt agatctaacc ccaatgcttg tgcagattcg atgactgcgg
     3361 tgttttttcc ttgccaagtt gtcaataaat agctgatttt tcgctgacgg aataagagat
     3421 ccatcccatc aatggtacgc catgttcccc tgagcgcgta atagattgct tctgcagtat
     3481 tttctccgcc atctgcaatc ggcacattga tttttcttcc tttacgatct tcccagccat
     3541 ttagaactgc ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg
     3601 ccaaaatatc catctgtatc cctcactttc ttcttaagta agacactttt tttcattttt
     3661 ttcaaacaaa acaatgctat tttcttccat ttttctatat actaaaaggg aactcattga
     3721 ataaaggaga gaaaaaacat gaaaatcgca atagctgggg ccggagcaat gggcagccga
     3781 atcggattga tgctacatca aagcggaaat gaggtcctct tgatcgatcg ttggcctgct
     3841 cacattgaag ccattcgtac aaatgggttg attgctgact ttaacggtaa agaagtggtc
     3901 gcaaaactgc caatctattc accagaagaa atcatcgaaa gcaacgaaca cgtggacttg
     3961 atcgtagcat taacaaaagc caatcaatta gatgatatgt tttgttcgat ccaatccatt
     4021 attactgaca atacttatgt tttgtgttta ctaaatggat taggtcatga agatgtactg
     4081 gaaaaatatg tgccaaagaa aaatatctta tttgggatca cgatgtggac agctggtcta
     4141 gctggtcccg gaaaagtcac attgttaggt gatggagaaa tcgaactaga gaacttggaa
     4201 ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat
     4261 ccgatctata gccataacgt tcgttattcc atttggcgaa aagcttgtgt aaatggaaca
     4321 ttgaatggtt tatgcacgat tttggattgt aatattgctg aactaggcgc gcaaaaagcg
     4381 gcagagagca tggttcgaac aatcgtatct gaatttgctt ctatcgcagc aaaagaagga
     4441 atcatactag atcaagaaga agtgtaccaa catattgctt ctacttatga tcctgacaac
     4501 atcgggttgc attacccatc tatgtaccaa gacctgatca aaaaccatcg tttgactgaa
     4561 atcgactaca tcaatggtgc catttggcga aaaggacaaa aatatgatat tgctacacca
     4621 tattgtgcct ttttgaccca attagtccat gcaaaagaag gaatccttgg cgcagaataa
     4681 aaggctctct gatgagaatc agctgaatca gcagctggtt ctcttttttt aagcccaata
     4741 aaagatcaaa aagaaaacac aactctcatg atgccataag aattgtgctt gtttgtttca
     4801 gtacataatc tcttcaaaat gtaggttttt tatttgtttt caacagagga gaggtcatat
     4861 tcgtattgct cataaatagt ggaatcgtta gtgataaatc gttttgccca attttctgca
     4921 tcataaaatt ccaatagctc tgataggatt ggcttaaatg gtgtatgcag aaattccggt
     4981 aattcatcta aatcacgttc tcgaagcaaa aaaatatttt caggacgata aaaatgataa
     5041 tgagcaatcg cttgattatc cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc
     5101 cgcaaagtca ctccttgctg accctcccgc aacagttcca gaatcccgcg cgattcccct
     5161 gtttttttca ggtactcttc ataagtaaga tatccaggtt gttcattccc tcgatgtgga
     5221 agaatgaaat agttggtcgt aacatctctt tttataaatt cctgcttata gctttctgct
     5281 cgttcttttc tcccattatt cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa
     5341 gaaaaattca tatgtttcga ataaaatgaa ctattatgtt tcagcccaag ttcttttgct
     5401 tccaaaaaat caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc
     5461 atcgcctcgt gttctgcttt gaagtgattc caaaaaaata acacaatttt tgcattggta
     5521 aatgggcgca cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc
     5581 tgataatttg ccaactgttg tctagctgat ttaatgaatt ccgactgtag taaatctttt
     5641 ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat aaagtgattt attttttgca
     5701 actagcagta cgtcattttt gttcatcttt tccgcctcac tttctctgtc actttttcct
     5761 atgatagcct gtttaaatag taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat
     5821 ttaacatttg tatgaaaata tgatgaaata aatcattctt ctattaaaat tcgcctaaaa
     5881 gttttctgaa tagttgtaat tgtctgaaaa tttgttatca ttagtcattg ttaaatattg
     5941 ctatttattc ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa
     6001 gataaaaaat tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga
     6061 gcaatcggtg caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct
     6121 gtattgattg cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga
     6181 aaaatcgttt taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat
     6241 ctaggtgctc attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat
     6301 atcatcgctg aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca
     6361 gcctgggtat ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta
     6421 cggttattcg cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta
     6481 ctgatcattt ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct
     6541 acccttcagc aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc
     6601 agttctttgc ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta
     6661 agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga
     6721 atcatttcct tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact
     6781 ttagcaggca cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt
     6841 gcagcggata tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt
     6901 gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga
     6961 aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt
     7021 gttctttatc ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg
     7081 gcaggttccc tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt
     7141 gtactggcac taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct
     7201 gtcttaggat tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg
     7261 actggcctgc tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta
     7321 ttgacgaatg aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct
     7381 attgaagaaa ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc
     7441 tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc
     7501 catgctcatg acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa
     7561 gtattggaag tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta
     7621 cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt
     7681 gatgatgggt gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa
     7741 caaaaagata aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt
     7801 tatggtacga agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca
     7861 tcattctatc gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa
     7921 attagcggaa gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat
     7981 caaagaaatg gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat
     8041 cagcttcaag aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa
     8101 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt
     8161 ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa
     8221 aatcagcgtt ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg
     8281 ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg
     8341 aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt
     8401 gtataaattt ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg
     8461 attgtgacaa aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt
     8521 cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc
     8581 actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag
     8641 tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta
     8701 gttgattact gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc
     8761 tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc
     8821 aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt
     8881 cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa
     8941 tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc
     9001 tccgcggcta agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc
     9061 gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac
     9121 tgctacagcg atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag
     9181 taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac
     9241 atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat
     9301 gaataaaaag aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg
     9361 tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga
     9421 catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa
     9481 gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc
     9541 actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg
     9601 gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca
     9661 tacagcaatc aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat
     9721 gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa
     9781 catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac
     9841 gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg
     9901 tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag
     9961 ttaagatatc ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa
    10021 agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc
    10081 tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta
    10141 ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg
    10201 tgaaaagttg acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa
    10261 atattttttc atttttctga aaattatttt tataactttc acacacaaga ttatttttta
    10321 ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat
    10381 tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc
    10441 tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt
    10501 gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg
    10561 gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc
    10621 atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac
    10681 gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat
    10741 cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt
    10801 tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc
    10861 tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt
    10921 gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc
    10981 tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc
    11041 tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa
    11101 aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg
    11161 ggtgccatat acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc
    11221 ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa
    11281 agcatcgaat tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc
    11341 atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa
    11401 ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg
    11461 gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg
    11521 gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt
    11581 tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt
    11641 gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc
    11701 ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa
    11761 gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac
    11821 tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc
    11881 tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc
    11941 cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc
    12001 attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga
    12061 ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc
    12121 tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc
    12181 tgctaaagcc acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt
    12241 ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc
    12301 tagttttacg atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc
    12361 caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc
    12421 tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa
    12481 aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat
    12541 accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag
    12601 tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt
    12661 ctaacaaata cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt
    12721 cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa
    12781 caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga
    12841 aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat
    12901 tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg
    12961 tcactactaa cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa
    13021 aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat
    13081 tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata
    13141 cgactatcta aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa
    13201 tccaatgctt ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac
    13261 ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg
    13321 ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact taattccacc
    13381 agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct
    13441 tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg
    13501 aagatctgga tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg
    13561 ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac
    13621 tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca
    13681 ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct
    13741 actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa
    13801 ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa
    13861 tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc
    13921 tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc
    13981 agattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca
    14041 cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt
    14101 tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat
    14161 acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca
    14221 agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca
    14281 agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg
    14341 cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata
    14401 gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact
    14461 taccatttca atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc
    14521 agtaaaagga cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc
    14581 aacatctcaa aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct
    14641 acttatcgac aaattattct ccaaggccat tttcagcttt tcttacttga ctcttctcac
    14701 cagccagagc ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt
    14761 ttgttgttgg gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta
    14821 ttttttctat atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc
    14881 ttttccttcc gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa
    14941 actggaatca ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac
    15001 taaagaatct tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt
    15061 cagataagga atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc
    15121 caagtaatca gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt
    15181 cgtagcaatg cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata
    15241 tggttttaag tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc
    15301 tcggacttcc tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg
    15361 tttccagttt tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg
    15421 ctgaaatccc caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc
    15481 taaatcaaat attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt
    15541 ttagcacgac tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt
    15601 tcttggaatc gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg
    15661 actgtagcaa ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca
    15721 ccaaaacttg taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac
    15781 atggcaaatg ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc
    15841 actaaggttt gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa
    15901 atcactcgct gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata
    15961 acaaaataca tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa
    16021 accatgaatt ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta
    16081 tccatccgat cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa
    16141 agttgtaaag gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac
    16201 atattgccaa aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt
    16261 tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg
    16321 tctgcagatt ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact
    16381 ttcattgcag gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta
    16441 cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg
    16501 tcttcagcta cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc
    16561 aattcattga tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc
    16621 ccagtcagaa cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt
    16681 gtttcttttg gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct
    16741 ttgactgaaa attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt
    16801 aagccatctt cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt
    16861 aatggtgtaa cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc
    16921 gcaccttttg tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga
    16981 cgacgttcaa aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct
    17041 aaagtttctg ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga
    17101 tagtagctat ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc
    17161 atatgatatt caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac
    17221 acatcgattg caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg
    17281 gccatcacac ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt
    17341 gttagaccaa ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta
    17401 agcccattga tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg
    17461 gatgttttat ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct
    17521 gctacatgac caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca
    17581 ctgatgacat tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg
    17641 tcttcacata ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg
    17701 gaaatgaata aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct
    17761 gaaagccgaa caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca
    17821 ttacgtttta cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg
    17881 gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg
    17941 gctacaccga aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa
    18001 ataattccta atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct
    18061 acaatgaatg gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa
    18121 cgttcgattt gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct
    18181 tgtttcgttt ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc
    18241 tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt
    18301 catcggatcg atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc
    18361 ctttcctcta gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac
    18421 taagtaattt taattcatga cttccaatca tttttcgaat agttattttt ccaattagac
    18481 cacctccttt ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa
    18541 tactcagcga aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag
    18601 ttttgacact gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct
    18661 tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta
    18721 tgtttgtata ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa
    18781 ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca
    18841 cgttatattc ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag
    18901 aaatatagta ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac
    18961 ggcgtgagac gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc
    19021 attgctgaaa tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac
    19081 ttgactgagc gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt
    19141 tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca
    19201 aaagctgccg atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa
    19261 aacatcattc acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc
    19321 ccgccattcg atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact
    19381 gctggtctag ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg
    19441 attctttcct aacatatatt cgagcactgt gaagaattct tggtagtctt tcgtcttttc
    19501 ttcccctaaa gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat
    19561 cactgtactt ctttctttat gtgctaagct ccgtttcata tcttcattca cactacgtgc
    19621 ctttttcaac cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag
    19681 atactgctca tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata
    19741 attgttctca atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc
    19801 aaattgacca tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg
    19861 taaaaattca aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac
    19921 caaataatac agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata
    19981 aggatcaaag ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat
    20041 tggttcatcc agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc
    20101 taaaaatgat ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac
    20161 tagagaagtc ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac
    20221 tcgaagtttc ttgatcaatt caccatcata cataatagtt ctccttttta atttaaattt
    20281 ttccacattc aataaataga tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat
    20341 gtttttttca taaaaaatac caaccttcaa gcattagaat gttgtctaac tactgaaggt
    20401 tggtttgatt gtttattgat tatttttttg ctgttcaacg tctgttcttg ttactggata
    20461 taagttggtt gttgttttat taccattttt tgaaaagaca cttgtcgaat gttcgccttt
    20521 ttgcttttcg attgcttcat tacgttccgc ttcattttta tagtcatatt ttttcggatc
    20581 aatcgtctca aatccgtttg gcacataaaa ccgcaagaga ttttcctgat tcagcttatc
    20641 tgaaagactt aacgcagtct tgactttttc ctgatctttg gagatttcct catcgattcc
    20701 tgctgctttc gtatcgatga tttctcctgt ctgattattg taagcttttc ctcctaggac
    20761 ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca actacttgat catgctgaga
    20821 agataataag tctgtaccaa aatgaagata atctttatca tcgattccca atagatgtag
    20881 taatgtcggc aaaacatcga tttctccacc gtattcatga ttgactgtcc ctttagtata
    20941 accaggcata tggatcatca atggcactcg ttgcatctgt gcgttgtcaa attcatccca
    21001 tgtatctgga tctttaccaa gagcgcttgc taaatcttta ttatctgtat tcgagatccc
    21061 aaaatgatcc ccgtaaatca caaagattga attttggtaa ataccagatt ttttcaaata
    21121 cgtaaaaaat tgttccaatg attgatccag ataatgtgcc gttcgaacat aattatctac
    21181 tgtgctattg ccagtattca aagatggaaa gtcgaaattc ttgtcatccg tataataagg
    21241 cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat gctcaagata
    21301 ttgggcagat tctctgaata aatctttatc taatatccca taacctaacg tttcgtcgga
    21361 ttcatcaaaa taagatcgat caaagaaatt gtcataacct aagtttttat atacatgatc
    21421 tctattccaa aaactagcta cattcccatg gaaaacagcg cttgtatagc cttgcttttg
    21481 tccaaggatc gctggtgcgg cttgaaagac attgtctgac ccaagttctg tgaataatga
    21541 gccttgaggt aaaccaaatg tgccagtttc caacatattt tcagcatcac ttgtctttcc
    21601 ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt gcttgattct ggaaaatact
    21661 atttaaaaac ggtgtgactt cttgtccgtc taccttcata ttaatcaaaa actgctggaa
    21721 actttctaaa tggagaacga tcacattttt ccctttagcg atcccaaatg tttcgggatt
    21781 tggctctgca taatgtttct ttgtataatc taaaacttca tcaatcccat tactgcttgc
    21841 atgcgcgcga acactgctag tcattcctgt tttgattccg tcataaatag tatacgcatc
    21901 gattcctaga tatttgacaa tatatgaacg gtcaaatgtt cgctgcagca attgtggacg
    21961 gttggcttcg ctcagcgcta gatttcctga aaaaataagc cctgaaagac aggtaacagc
    22021 gatagccatc ggtttgccga cagggttgct tttgataggt attttcttga ccttcaacca
    22081 aaccagtagc ccaatgaaca cagcgatatc cagccagtag atgacatcat ggggcttcat
    22141 caaagaaaag gagcttcctg acaatccttg cgacactttc gaaaagccta atacagattt
    22201 gactgtaatg aaatctgtga attcccgata aaaaataata ttcaagtaca acaaaagcgt
    22261 atttaaaata tcaatgatca aaagaatagc taacgctggc tttggctttt taatatacaa
    22321 agaaaggcca aaaaacaata aggtagtcgc aattgggttg atccacaaga taaagtactg
    22381 gatcgtacct tccacaccta atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca
    22441 gaaaaagaaa acagctgtca aaacgattgc taaacgattt tggcacaatt ttttaagttg
    22501 tgtcacgtat atcacttctc tctattcaca ttttttctta ttttgctcct gaaaaatgaa
    22561 aatttcctaa aaaagttgtt atcttaagat ttttttattc tttctaaaga ttattccgtc
    22621 cacttttcac agcataaaac cgctgcgatg taagcgatta aactaatgaa gagataaaag
    22681 tcttgtggtg accgcttcac tccacggtat tcaaaagcca gtttctcggt attcgtacaa
    22741 aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat ttcttgatgc aggacagctt
    22801 tttcacaact tcttccttaa acggtgttct cctgccaact cctcggccac aagtcacaat
    22861 atttataaaa catgagaagc tgttttttga aattgttcct tgtggctcag agttaaacgg
    22921 caggctcaag acctcttgat acacggtgtt caatcagctg accctcggta ttagattatg
    22981 tcaggacgct aattttacaa cctcttggta aacggtgttc aaaagcttgc atctgcggta
    23041 ataagcccaa ccaaacgaaa aatataagga gctatttttt gaaattgctc cttgtggctc
    23101 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccttcgg
    23161 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
    23221 caggtcaaaa cgctgatttc acaacctctg tttttcattg tccataggat tgatattgtc
    23281 cttgtgatga ctcattcgta ataattgagg cttgattttc ttcacttccc gttatcgttt
    23341 gtccactgcc agaatctgaa ggacttgttc ctgtcgactc gtttttttga gaagagacag
    23401 ttgttttctt gacttgttca tttgtctttt tccattccgc taaacgatcg gcgaccactt
    23461 ttttgtctac tttttcagaa taagcggtga tcacttttcc atttttcaaa cgggctactt
    23521 tgtcgccatc atgagtcaaa agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc
    23581 tgagagaaat cgcactaagt acgtcatcga tattaataaa ttgattttct tcttttcctt
    23641 ttggcaaata aatgtccaat tcgccttttt cgtaattggc agttatttta tactccgccc
    23701 accaataagt tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt
    23761 tttctgtttc tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa
    23821 taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc
    23881 tttcctccac tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag
    23941 acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt tttcacaaaa tggtaagaat
    24001 ggagaatttc acgcatttgc tctcaactat tctttcttaa gcctcaaatc cgtttttatc
    24061 tgatacgtct ttgaaccaat gacccgattt tttgattgtt ctttttccat cttgtgctaa
    24121 gtctactgaa ataaagccat atcgattctt atatgcattg gtccacgacc agttatccat
    24181 acatgtccac atatgatagc ctaaacaatt ggcaccttct tgaatggctt gatggacata
    24241 tttcagatga gattggacaa attcgatccg ataatcatcg tggatcatgc catcttcttt
    24301 gataaatcgt gcttcgtttt ctacacccat accgttttca gaaataaagc agtcgatatt
    24361 tccataatga tctcgtacat tgatcaaaat atcatagatt ccttgttcat agatctccca
    24421 tccgcgataa ggattcattt tacgaccagg catttcataa ttgtcgaaat aatcctcagg
    24481 cattggaccg tttttagtat cgattggtgt ttcttttgct tttacccggc gtggctggta
    24541 ataattgata cctaaaagat ctactgtatt ttctttgata accgtcaaat cctctgcatg
    24601 gatcacaggc atgagatcaa gttcttttac tatctcaatc aattcacttg gaaactcgcc
    24661 ttttacagct ggatcaagga atgaacgatt gaagaacgca tctgctattt gcgctgcttt
    24721 aacatcttca gggtttgatt cgtctcttgg atagcttggt gtcagattca agatgatacc
    24781 aataacacca ccttgctcca tttcatggta agatttgatg gctaacgctg aagccaacgc
    24841 ttcatgatag ccaacttgga tcgctttttt catatctgac tcttctggat aatgccatcg
    24901 atataaataa cctgcttcga ctggaacgat tggttcatta tgagtaaacc atttttttac
    24961 tttcgaacca aacaattcaa aacaactttt ggcatatttt tcataggctt caaccgtttc
    25021 tcggttcaac catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt
    25081 cacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac
    25141 ggcttcttcg ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagatgt
    25201 tcgaaatgaa ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata
    25261 aacttgcgag gttttttcag gtcctacttg atcaaagaac ttttgcggtt cttttttgta
    25321 ccaataatca aaaatgttct ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg
    25381 gccacttgct gcagaacccc accaaaaatt tgctggaaat tgatattttt tcatcaaagc
    25441 acctcttttt cttttttagt tcgttttagt tgattccatg tatgatattt tccatcacaa
    25501 tcactttttc attttcttcc tcttcttttt tgattcgttt taacagctgt tctgctgctc
    25561 gttccccgat tttatctgtt ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt
    25621 ttttatctaa ataatcatag tgactgaacg caatatcctt cccgattctg acaccttctt
    25681 gattgaaaca ttcgatggca ccaatcgaca tattgtaatt gctgacaaaa atagcagttg
    25741 cccctttttc taataatgct tttgccccgt aatagccgct ttttctagaa tagtctcctg
    25801 taaagacctc taattggatc gatggatcat tttggaccgc ttctttggct cctgctaaac
    25861 gttctcttgc tacgtagtct gtttgagtgg cagcaataaa tccgattttt ttatgtcctt
    25921 gttcaccatg atgagcgatc acttttgcta cactttctcg atcattcact aatattgtat
    25981 caatacctgc tggtccttct ggacaattca atgcgataac tggaatactc atttttgtga
    26041 gcatttcaaa tccagaccac tcttcttcgg ataagaagac aatcagccca tcgatcacat
    26101 gcttcatcag gtattcaatt ttttgttcaa ttttttctgg ctgatcggca aacccgctta
    26161 ataataagct atacccattt tcttcagcta ctctttcgat ccctgagaca acttccgacc
    26221 caaaacggct ggaaatcccg tttatcaaaa gtccaataga gaagcttcga ttattcttca
    26281 accctttagc aatgatgttt tctttatagt ccaacgctga aatcgcttct gctatttttt
    26341 tcatgttctt ttccttcagt tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa
    26401 caccagccat cttagccaca tcacggatcg atgccataca ttctcactcc tgccagttac
    26461 atttgatttc tctttcttgt tgttcgatta tgatgaccgc attctttgca tagtgttgct
    26521 tgtcatcaat atccatataa ctaaagtctt cagtagagca acttcctctc actattaatt
    26581 atacttaaac aagtatttga accggttcat ttttaatata gcattttttt aaaagattgc
    26641 aacacttttt taaaacgctt tctgaaagtg gcggattttt tgccttttta aaattttatt
    26701 ctaaaattta ctctatttgt gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt
    26761 ttacattcca atggtataat ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat
    26821 taatcgtact atttattttg tctgcgggac ttttgacatt gactggctgt gatttgtttg
    26881 gcggctctga ccaatcctcg gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag
    26941 cagataccac caacagtgta tatacgctaa aagtacgtaa agatatggat ttatccgcgg
    27001 gacagttgac atttacagat aaaaaaatgg aatggacacg cacgtatcag acacaaggaa
    27061 ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac agatatcaag atcactacca
    27121 aagatgacga atatattatt tcaggaaaag aaaataaaaa agaaacaaca gtcatattta
    27181 caaaaatcgg taattaccgt ttgaaggatg aggatggcaa catctacagt ctatagtgcc
    27241 gtctgttgga tcttcttttg tttctcccct tcttttctgc tatagtaaag aagcaagatg
    27301 attgtaaagg agacgaaatg gatgatcgat agtcctgatt attgtacatg taataacaac
    27361 aaaaatatcc atacagaatt taatgaccaa ggaagcttga gcatatgcga caactgtcac
    27421 aaactagtca aagattcatt taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa
    27481 agtagaagaa gataaacaaa tccaagcaaa actcactatg gctgattttt atgaacttca
    27541 aactcagctt cttcctttga tcaagcttca tcagtaaaac aaacagtgga tcatttcagt
    27601 ttacaaatca aagagtaaag atttatttat ttcaaatatt tgaatataaa ataaccaaac
    27661 gatagaaagg cttattctac atcacgtagt a
//
LOCUS       DAZUKT010000032        27580 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000032 DAZUKT010000000
VERSION     DAZUKT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.032"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1916
                     /locus_tag="ACG816_001376"
     CDS             150..1916
                     /locus_tag="ACG816_001376"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4920259.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2018..3277
                     /locus_tag="ACG816_001377"
     CDS             2018..3277
                     /locus_tag="ACG816_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4920260.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3424..4725
                     /locus_tag="ACG816_001378"
     CDS             3424..4725
                     /locus_tag="ACG816_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4920261.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4728..5579
                     /locus_tag="ACG816_001379"
     CDS             4728..5579
                     /locus_tag="ACG816_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4920262.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5592..6407
                     /locus_tag="ACG816_001380"
     CDS             5592..6407
                     /locus_tag="ACG816_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4920263.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6558..7724
                     /locus_tag="ACG816_001381"
     CDS             6558..7724
                     /locus_tag="ACG816_001381"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4920264.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7954..8601
                     /locus_tag="ACG816_001382"
     CDS             7954..8601
                     /locus_tag="ACG816_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4920265.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8685..9629
                     /locus_tag="ACG816_001383"
     CDS             8685..9629
                     /locus_tag="ACG816_001383"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4920266.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9764..11215
                     /locus_tag="ACG816_001384"
     CDS             9764..11215
                     /locus_tag="ACG816_001384"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4920267.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11236..12072
                     /locus_tag="ACG816_001385"
     CDS             11236..12072
                     /locus_tag="ACG816_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4920268.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12072..12731
                     /locus_tag="ACG816_001386"
     CDS             12072..12731
                     /locus_tag="ACG816_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4920269.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12857..13546
                     /locus_tag="ACG816_001387"
     CDS             12857..13546
                     /locus_tag="ACG816_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920270.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13552..14508
                     /locus_tag="ACG816_001388"
     CDS             13552..14508
                     /locus_tag="ACG816_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4920271.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14552..15064
                     /locus_tag="ACG816_001389"
     CDS             14552..15064
                     /locus_tag="ACG816_001389"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4920272.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15320..15868)
                     /locus_tag="ACG816_001390"
     CDS             complement(15320..15868)
                     /locus_tag="ACG816_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4920273.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15957..17138)
                     /locus_tag="ACG816_001391"
     CDS             complement(15957..17138)
                     /locus_tag="ACG816_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS4920274.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17374..18882)
                     /locus_tag="ACG816_001392"
     CDS             complement(17374..18882)
                     /locus_tag="ACG816_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4920275.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19041..19232
                     /locus_tag="ACG816_001393"
     CDS             19041..19232
                     /locus_tag="ACG816_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920276.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19435..20352
                     /locus_tag="ACG816_001394"
     CDS             19435..20352
                     /locus_tag="ACG816_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4920277.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20345..21418
                     /locus_tag="ACG816_001395"
     CDS             20345..21418
                     /locus_tag="ACG816_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920278.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21566..22084
                     /locus_tag="ACG816_001396"
     CDS             21566..22084
                     /locus_tag="ACG816_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920279.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22065..22457
                     /locus_tag="ACG816_001397"
     CDS             22065..22457
                     /locus_tag="ACG816_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4920280.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22533..23468
                     /locus_tag="ACG816_001398"
     CDS             22533..23468
                     /locus_tag="ACG816_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4920281.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23601..24380
                     /locus_tag="ACG816_001399"
     CDS             23601..24380
                     /locus_tag="ACG816_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4920282.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24373..24690
                     /locus_tag="ACG816_001400"
     CDS             24373..24690
                     /locus_tag="ACG816_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4920283.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24934..26544
                     /locus_tag="ACG816_001401"
     CDS             24934..26544
                     /locus_tag="ACG816_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4920284.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26701..27549
                     /locus_tag="ACG816_001402"
     CDS             26701..27549
                     /locus_tag="ACG816_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4920285.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8922 a         4881 c         5539 g         8238 t
ORIGIN      
        1 aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac
       61 tgttaccgat aacatttttg aaagcgttgt cttttgtttc tctatcactt atagtgatga
      121 cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg
      181 aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga
      241 aaaatgatat caaaaaagta tatgtgcttt gtggagatcc atataccata acaactgaaa
      301 aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt
      361 gggaaattga agtgacgagc gaaacaaggc gcttacagta tgcgttccat gttgttgggg
      421 aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga
      481 ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta
      541 ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg
      601 gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag
      661 aagacttctt cgggggagat ttgcaaggtg tgagggatca tttggattac ttgacggacc
      721 ttggcgtcaa tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg
      781 atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact
      841 tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata
      901 tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata
      961 aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag
     1021 aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa
     1081 atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag
     1141 aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga
     1201 aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt
     1261 ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat
     1321 tcacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca
     1381 gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact
     1441 tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga
     1501 tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga
     1561 cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga
     1621 aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac
     1681 agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtatat gatcgtgaag
     1741 ataagctcta tttcacacgg gaactagagg gacagatcat ctatgtttac tttaatcagt
     1801 caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt
     1861 tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac
     1921 tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg
     1981 gtgtaacagc acgttgaata cacaggagga aatgaagatg aaaaaaagaa gtgtgaaaca
     2041 gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg
     2101 taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc
     2161 tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa
     2221 aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc
     2281 tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca
     2341 gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa
     2401 agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt
     2461 tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa
     2521 tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt
     2581 agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt
     2641 atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga
     2701 aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat
     2761 aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat
     2821 tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa
     2881 aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt
     2941 agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa
     3001 tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc
     3061 tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga
     3121 aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa
     3181 cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt
     3241 aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt
     3301 tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct
     3361 tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt
     3421 cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt
     3481 attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc
     3541 atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg
     3601 gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact
     3661 ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct
     3721 tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc
     3781 atacataata taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc
     3841 atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat
     3901 gacttcaatc atattccacc agccagttta attgattggg taggggctaa aaactttttt
     3961 agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg
     4021 atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt
     4081 gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg
     4141 gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt
     4201 gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca
     4261 atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg
     4321 ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac
     4381 ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg
     4441 cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc
     4501 aataatttct ccatgatcta tctgtttaac aatggtggtc ccggaagcgt aggcggagga
     4561 gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg
     4621 cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca
     4681 ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc
     4741 attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa
     4801 tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag
     4861 gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga aactttactc
     4921 ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc
     4981 taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc
     5041 gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta
     5101 tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact
     5161 ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag
     5221 gatactttga cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc
     5281 atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag
     5341 cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa
     5401 cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc
     5461 aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat
     5521 tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg
     5581 agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc
     5641 aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt
     5701 tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc
     5761 cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt
     5821 ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc
     5881 ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta
     5941 ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct
     6001 atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc
     6061 aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata
     6121 gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct
     6181 ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg
     6241 caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta
     6301 tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt
     6361 tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga
     6421 tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga
     6481 aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt
     6541 ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt
     6601 tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg
     6661 acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa
     6721 gtaacgcttc tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa
     6781 gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag
     6841 attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata
     6901 tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga
     6961 cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata
     7021 aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg
     7081 gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg
     7141 ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt
     7201 tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg
     7261 atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt
     7321 tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc
     7381 gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg
     7441 tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt
     7501 tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc
     7561 aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat
     7621 atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc
     7681 ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact
     7741 gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg
     7801 tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa
     7861 agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata
     7921 gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt
     7981 ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg
     8041 aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca
     8101 agcacatctt cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc
     8161 aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt
     8221 actagtttgg aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat
     8281 gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat
     8341 ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat
     8401 gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc
     8461 ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa
     8521 gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg
     8581 tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt
     8641 catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga
     8701 cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga
     8761 ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct
     8821 gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga
     8881 gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg
     8941 cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga
     9001 gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca
     9061 ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg
     9121 aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca
     9181 aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt
     9241 ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag
     9301 aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata
     9361 tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc
     9421 aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg
     9481 gcgcaagcat gttacagatt ggtacagagc ttcataaaga agggccggaa attttttcta
     9541 gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat
     9601 ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt
     9661 ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta
     9721 gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga
     9781 attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca
     9841 gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga
     9901 gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc
     9961 atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg
    10021 tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat
    10081 aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga
    10141 cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc
    10201 aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga
    10261 tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc
    10321 gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga
    10381 tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat
    10441 ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa
    10501 acggttgaag atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc
    10561 agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga
    10621 gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc
    10681 agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga
    10741 agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac
    10801 ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca
    10861 agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga
    10921 tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga
    10981 aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca
    11041 gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa
    11101 catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat
    11161 cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa
    11221 acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt
    11281 attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta
    11341 gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat
    11401 aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg
    11461 ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca
    11521 aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa
    11581 ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat
    11641 gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat
    11701 actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc
    11761 acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat
    11821 tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag
    11881 aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg
    11941 aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata
    12001 tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg
    12061 gtgagtgagt gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt
    12121 tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa
    12181 atttggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat
    12241 ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt
    12301 tatatttcgg gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga
    12361 ttaccagcag tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag
    12421 gtcagtggga cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg
    12481 ttttgccgca agcttttcga atctcagtac cggcactcag taacgtagct atggatctag
    12541 tgaaaggtac ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga
    12601 tcataggcgg tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt
    12661 gggtcatttg tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatccatc
    12721 agtcaatctg aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt
    12781 cttgtatatt ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg
    12841 ttcggaggta gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca
    12901 atattgcatc gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg
    12961 aattgtagat acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta
    13021 tgagctgaaa gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca
    13081 ttataattat tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac
    13141 aggaataggt gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg
    13201 tacatttctg attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt
    13261 gacaaaccgc ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg
    13321 aggcgtaaaa tattttccaa aagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa
    13381 tcaagatcca ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca
    13441 ggcaatcaat gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag
    13501 gcaacaacag cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat
    13561 ccttatttaa gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga
    13621 ttgctctcca ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa
    13681 aatgtgatcg aattatttca aaaaaagcac catccaatcg ctgtgatcca aaatccccaa
    13741 gtaggacaat tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc
    13801 tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct
    13861 ccagcgtttc ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag
    13921 ttggcaggag ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg
    13981 cccgaaaaca aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat
    14041 gcagataaaa cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat
    14101 attggatttt ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc
    14161 cgagcgcttg ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat
    14221 tttgtctctg acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga
    14281 gataaaatga aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg
    14341 attgaattat tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag
    14401 tggtcactta tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg
    14461 cttaaatatt attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta
    14521 aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga
    14581 agtagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc
    14641 tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag
    14701 aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc
    14761 aggcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta
    14821 tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc
    14881 gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga
    14941 tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca
    15001 agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa
    15061 ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca
    15121 tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc
    15181 gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg
    15241 aaactccctt catcgagagg tcgtgacata gcttttgtca cgatctctcg gcttatccta
    15301 cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt
    15361 ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact
    15421 gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac
    15481 gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta
    15541 ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat
    15601 ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat
    15661 attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa
    15721 caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa
    15781 tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag
    15841 gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga
    15901 aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtattttat
    15961 accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt
    16021 ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca
    16081 gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct
    16141 gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca
    16201 tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt
    16261 ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg
    16321 aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata
    16381 gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa
    16441 aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag
    16501 ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc
    16561 acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac
    16621 atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt
    16681 atggaaataa aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc
    16741 aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga
    16801 agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat
    16861 acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca
    16921 ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct
    16981 gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca
    17041 attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat
    17101 gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt
    17161 ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact
    17221 tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt
    17281 taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata
    17341 gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg
    17401 gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt
    17461 attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc
    17521 aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat
    17581 gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag
    17641 aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt
    17701 taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc
    17761 atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat
    17821 cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac
    17881 ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa
    17941 gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac
    18001 ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata
    18061 aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac
    18121 atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat
    18181 attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa
    18241 cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg
    18301 gctttcatga tacagttggt tcaagcaagt agcgagcttt ccacttcctg ggccaggagc
    18361 tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc
    18421 tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt
    18481 gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg
    18541 agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa
    18601 gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc
    18661 agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt
    18721 cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctaacttccc
    18781 accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg
    18841 ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt
    18901 actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa
    18961 aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt
    19021 tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact
    19081 atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat
    19141 tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc
    19201 atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga
    19261 ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt
    19321 gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg
    19381 tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa
    19441 gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa cgcggatcag
    19501 gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt
    19561 tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct
    19621 gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa
    19681 aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt
    19741 ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact
    19801 tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg
    19861 cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa
    19921 aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat
    19981 gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg
    20041 aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct
    20101 aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa
    20161 gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa
    20221 caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa
    20281 ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc
    20341 gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca
    20401 tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga
    20461 aatccctatg ctagaggaaa caaaaataca tgaaaaagaa aaagtaactc cgacgaatac
    20521 gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg
    20581 aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc
    20641 ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata
    20701 tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga
    20761 atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc
    20821 agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa
    20881 cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat
    20941 gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct
    21001 ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt
    21061 caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca
    21121 gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac
    21181 gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga
    21241 aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac
    21301 aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt
    21361 cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt
    21421 ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt
    21481 tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc
    21541 tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt
    21601 gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat
    21661 gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat
    21721 gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca
    21781 atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt
    21841 gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat
    21901 aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct
    21961 tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt
    22021 tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt
    22081 atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata
    22141 cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt
    22201 tacaagaaaa tggtcaaaac ttttcggaaa aagaagtggt cttcaaaaaa ggcgatgatt
    22261 tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc
    22321 ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga
    22381 aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca
    22441 atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca
    22501 gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt
    22561 tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct
    22621 atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca
    22681 gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc
    22741 atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt
    22801 tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc
    22861 atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag
    22921 gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca
    22981 cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact
    23041 tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg
    23101 ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg
    23161 ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg
    23221 atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa
    23281 aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta
    23341 acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc
    23401 ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca aattattctc
    23461 gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt
    23521 aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg
    23581 taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc
    23641 atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt
    23701 tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg
    23761 gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat
    23821 tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt
    23881 ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa
    23941 actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt
    24001 tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt
    24061 tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca
    24121 ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag
    24181 gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa
    24241 cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta
    24301 agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg
    24361 aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg
    24421 gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg
    24481 ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat
    24541 gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt
    24601 ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt
    24661 gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg
    24721 cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg
    24781 caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta
    24841 ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt
    24901 ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca
    24961 ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc
    25021 tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt
    25081 ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt
    25141 gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt
    25201 agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta
    25261 tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct
    25321 atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat
    25381 ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg
    25441 cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata
    25501 ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc
    25561 catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc
    25621 agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta
    25681 ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg
    25741 aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc
    25801 agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca
    25861 gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc
    25921 gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact
    25981 ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt
    26041 gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta
    26101 tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca
    26161 attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg
    26221 agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc
    26281 aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc
    26341 tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg
    26401 tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc
    26461 ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa
    26521 catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg
    26581 gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat
    26641 cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac
    26701 atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat
    26761 cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga
    26821 atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact
    26881 acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg
    26941 tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta
    27001 tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc taagatcact
    27061 agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc
    27121 cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctatgc ttatctgatt
    27181 gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt
    27241 ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc
    27301 gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga
    27361 aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta
    27421 gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt
    27481 catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca
    27541 gcgcaataat tccttttatt caaaaaacat accaaaaata
//
LOCUS       DAZUKT010000033        27403 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000033 DAZUKT010000000
VERSION     DAZUKT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.033"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..1623
                     /locus_tag="ACG816_001403"
     CDS             145..1623
                     /locus_tag="ACG816_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920286.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1900..3960
                     /locus_tag="ACG816_001404"
     CDS             1900..3960
                     /locus_tag="ACG816_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS4920287.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4043..4612
                     /locus_tag="ACG816_001405"
     CDS             4043..4612
                     /locus_tag="ACG816_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4920288.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4787..5593)
                     /locus_tag="ACG816_001406"
     CDS             complement(4787..5593)
                     /locus_tag="ACG816_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920289.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5731..7245
                     /locus_tag="ACG816_001407"
     CDS             5731..7245
                     /locus_tag="ACG816_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4920290.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7648..8631)
                     /locus_tag="ACG816_001408"
     CDS             complement(7648..8631)
                     /locus_tag="ACG816_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4920291.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8636..9283)
                     /locus_tag="ACG816_001409"
     CDS             complement(8636..9283)
                     /locus_tag="ACG816_001409"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4920292.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9368..10000)
                     /locus_tag="ACG816_001410"
     CDS             complement(9368..10000)
                     /locus_tag="ACG816_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4920293.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10016..10567)
                     /gene="coaC"
                     /locus_tag="ACG816_001411"
     CDS             complement(10016..10567)
                     /gene="coaC"
                     /locus_tag="ACG816_001411"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4920294.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10564..11301)
                     /locus_tag="ACG816_001412"
     CDS             complement(10564..11301)
                     /locus_tag="ACG816_001412"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4920295.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11298..12287)
                     /locus_tag="ACG816_001413"
     CDS             complement(11298..12287)
                     /locus_tag="ACG816_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4920296.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12545..13087
                     /locus_tag="ACG816_001414"
     CDS             12545..13087
                     /locus_tag="ACG816_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4920297.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13104..14189
                     /locus_tag="ACG816_001415"
     CDS             13104..14189
                     /locus_tag="ACG816_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920298.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14201..15007
                     /locus_tag="ACG816_001416"
     CDS             14201..15007
                     /locus_tag="ACG816_001416"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920299.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15008..15835
                     /locus_tag="ACG816_001417"
     CDS             15008..15835
                     /locus_tag="ACG816_001417"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920300.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15832..16905
                     /locus_tag="ACG816_001418"
     CDS             15832..16905
                     /locus_tag="ACG816_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4920301.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17333..20029
                     /locus_tag="ACG816_001419"
     CDS             17333..20029
                     /locus_tag="ACG816_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4920302.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20094..20594
                     /locus_tag="ACG816_001420"
     CDS             20094..20594
                     /locus_tag="ACG816_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4920303.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            20606..21100
                     /locus_tag="ACG816_001421"
     CDS             20606..21100
                     /locus_tag="ACG816_001421"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4920304.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21121..21930
                     /locus_tag="ACG816_001422"
     CDS             21121..21930
                     /locus_tag="ACG816_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4920305.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            21927..22748
                     /locus_tag="ACG816_001423"
     CDS             21927..22748
                     /locus_tag="ACG816_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4920306.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22799..24898
                     /locus_tag="ACG816_001424"
     CDS             22799..24898
                     /locus_tag="ACG816_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4920307.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            24895..26013
                     /locus_tag="ACG816_001425"
     CDS             24895..26013
                     /locus_tag="ACG816_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4920308.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26052..26930
                     /locus_tag="ACG816_001426"
     CDS             26052..26930
                     /locus_tag="ACG816_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4920309.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27076..27273
                     /locus_tag="ACG816_001427"
     CDS             27076..27273
                     /locus_tag="ACG816_001427"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4920310.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8765 a         4696 c         5730 g         8212 t
ORIGIN      
        1 caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca agcataatcc
       61 atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag taattggtat
      121 gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga ggaagataga
      181 gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt agtgctgcct
      241 aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat catagaatta
      301 aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga agaagcagag
      361 atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat ctactttcaa
      421 cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac gatcgaaaaa
      481 tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag agaattagtc
      541 tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg ggaagaaata
      601 aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt ttctatgcca
      661 tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga atatctgttc
      721 aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac tattagtttg
      781 ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga atactttaag
      841 agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct tcatatccct
      901 aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac ggaagaagaa
      961 tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat ccacatgatt
     1021 agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga aatctctgaa
     1081 gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt caatcgaaaa
     1141 aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct acagtttttc
     1201 ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga gaataaacgc
     1261 tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta tctcttttta
     1321 ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg tattgatttt
     1381 tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt taaaaattac
     1441 aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc tgattgttta
     1501 cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc agatgactgg
     1561 gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga aaaaatcagc
     1621 tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat gtttttttca
     1681 aagaaaaggt aactagatat aactagatat aactagatat attacttaac aaaattaaaa
     1741 agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa aaatactctc
     1801 ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat gaaaattact
     1861 acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa aagacgttca
     1921 aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac
     1981 tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt ctatatgcat
     2041 gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt ttctgagacc
     2101 actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa acagctactt
     2161 cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca ctactggtta
     2221 attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct ttctatcttt
     2281 tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga tattttgaat
     2341 catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt atatgaaaaa
     2401 gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa agtttcgata
     2461 tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat gtccaaagta
     2521 atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac tatttccttg
     2581 tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt
     2641 gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga gagttacgcc
     2701 cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa
     2761 atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt tgtattaact
     2821 tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta tgtagaagca
     2881 tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa taaagaactt
     2941 caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct acctgctgaa
     3001 ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg gaaagtcact
     3061 tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg gtcggagatc
     3121 cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg ggaaattacc
     3181 tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct tattgttcat
     3241 ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc cttaggtaaa
     3301 cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga ggatggttat
     3361 ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc gtattcactc
     3421 caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca gatagatcaa
     3481 aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat ggctcatgca
     3541 gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag aatatctaga
     3601 aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc acaagtttct
     3661 ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa agaatttgtt
     3721 gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc
     3781 ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact tcctagtccg
     3841 ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt catgcgaggt
     3901 atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga ctggttctga
     3961 tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat ttggcataat
     4021 ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag aaatctggat
     4081 gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt ttgaatttcg
     4141 attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg ttggggaatt
     4201 tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt atcctgaaga
     4261 acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag caacctgtac
     4321 cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt tagcagtgaa
     4381 accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct tgcaagaatt
     4441 tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga gccatcaggc
     4501 gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag acggcacacc
     4561 taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg
     4621 tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat atcttacttt
     4681 tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa gaaggggcag
     4741 tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat cgtttgattt
     4801 ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga ctatagaggt
     4861 aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc ttcatagtat
     4921 ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt ttaccatctg
     4981 cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca gtgtttccgt
     5041 ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc atatcgaatt
     5101 gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact gtcatcgaaa
     5161 aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt gtatttttat
     5221 tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg aatgcatgta
     5281 gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct aaaactgttg
     5341 aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt atctttaata
     5401 tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa agcattgtat
     5461 ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg atacgtaata
     5521 aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta aaaaaggttt
     5581 tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa gaagcgtgtg
     5641 tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa aaatcactta
     5701 atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg tttattaaca
     5761 gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca
     5821 agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca agtatataca
     5881 gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg tggtgcaggg
     5941 atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt ggaaaagatg
     6001 cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc agaaacaaaa
     6061 tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga aacgctaaaa
     6121 ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca ctctgctgat
     6181 gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat cactggaggg
     6241 atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg atacttagtg
     6301 gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg
     6361 acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa
     6421 gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt tggtgccagc
     6481 aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac aaatcaagaa
     6541 ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac cgttgatatg
     6601 gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat tggtgaggtt
     6661 gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt caatgaaatg
     6721 gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc agcttatttg
     6781 atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga ccaaaaagga
     6841 tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt accaaaagaa
     6901 acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa agatgctcaa
     6961 ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa
     7021 atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta
     7081 ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt cacaggtggc
     7141 ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat ttttgggcga
     7201 caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag aacgtaaata
     7261 aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga ttgaacactg
     7321 tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa
     7381 aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa gcttttgaac
     7441 accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
     7501 aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg aaattcacaa
     7561 aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc
     7621 ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga tttttccgat
     7681 gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt ggaatgtacg
     7741 gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt gcataaaagt
     7801 ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac caagcagttg
     7861 gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc cacctagcat
     7921 accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt cccagtaaga
     7981 agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt ttcgtacctc
     8041 ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt ctttacaaaa
     8101 ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa gctgtctagc
     8161 taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga cagtttcgcc
     8221 ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa cttctgagat
     8281 tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat cggcaggcat
     8341 cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg tcccaggagt
     8401 aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga ctcccaaaat
     8461 agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc gattgacagc
     8521 tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag gcacttcaat
     8581 ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca tggatctatt
     8641 cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca gaaagtacta
     8701 gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca gcaactgtag
     8761 agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg gaagtaactt
     8821 cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta gttcgtttca
     8881 gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca ggccattttg
     8941 ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc gcattttcag
     9001 ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc tttccttgag
     9061 agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt ttttccacgg
     9121 accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat gtttgggaag
     9181 caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct tttatcaggt
     9241 gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc atcctttcga
     9301 cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag atcggctacg
     9361 atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca ttgcatactg
     9421 ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa ataaccgcta
     9481 gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa tcttgctgga
     9541 ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg gaaagtccag
     9601 ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga cctttataaa
     9661 cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc aaggctttcc
     9721 agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa gacaaaggtg
     9781 tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca agaaaagcac
     9841 ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg gttgttgcat
     9901 tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc aaaatagcta
     9961 aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct tttttctaag
    10021 catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata tcagctaagg
    10081 caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt tcctgatagc
    10141 cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac atattcgtgt
    10201 tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg actgttgaaa
    10261 gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta gcaggcgcga
    10321 tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc ggatttgatt
    10381 cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt aatggcgtga
    10441 taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat ttgacgaatt
    10501 gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct agtaagatct
    10561 ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa tctcagattt
    10621 actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt gtgtctcgcc
    10681 atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta tcagactatt
    10741 ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga aaccaatgat
    10801 ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt tcttcaaaac
    10861 gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg tctgttcatt
    10921 ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt tttttgccag
    10981 ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac tgtggataat
    11041 cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt ccagtgcaga
    11101 ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt gtttcgtagt
    11161 cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg cttgccctaa
    11221 tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag tcgttcctcc
    11281 agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct tccttggtgt
    11341 gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat ctcttggacg
    11401 accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag acgttgatct
    11461 attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat aaaaggcaag
    11521 acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc accgttataa
    11581 gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat gacatagtcg
    11641 atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg tcgtacgact
    11701 tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga aaaggtaatg
    11761 attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac accgccagaa
    11821 gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa agcgttcaca
    11881 cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag cgtcatattt
    11941 tttggtagag gaactaacca gtctcctggt actttttgat attggctata accgcctgga
    12001 tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga aaaacgtgga
    12061 ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg atatgttcgt
    12121 atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc agaataggca
    12181 atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact gattgctgta
    12241 tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg tgagacactc
    12301 cttttaaaat tttcttttat cataccataa agcctactct tttattcttg ttcttccgta
    12361 aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt attccaaaag
    12421 ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa taaatttcgt
    12481 atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg attcggagga
    12541 aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt tgacacaaga
    12601 agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg aacgagattt
    12661 aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg tgactccaga
    12721 agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag aagatcatac
    12781 ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg attcgaatga
    12841 aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata agacgtttga
    12901 accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt taagcttagg
    12961 agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga caaaacaaca
    13021 tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa cgcaatcata
    13081 tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat caagtagtga
    13141 aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa caaggaaaat
    13201 tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt attatagctg
    13261 gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg aatgatttgc
    13321 cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt cctcatatga
    13381 atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa tcagatatcg
    13441 aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa aaacgggaga
    13501 ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca attgtaaatg
    13561 aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa ctacgtacag
    13621 atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt atttttgtta
    13681 ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg aataaaggga
    13741 aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat catttcgttg
    13801 ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat aatctagttg
    13861 agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat gaaccagttg
    13921 aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga aaactagtcg
    13981 taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt tatgatgaac
    14041 agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc aaagtaggat
    14101 tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa gaagaattcg
    14161 atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa tgcgcaggat
    14221 ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg tattaatgat
    14281 catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt atctatctta
    14341 ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg catttttgat
    14401 cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa aactgaagca
    14461 caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc tgcttaaagc
    14521 ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct ttgattttat
    14581 cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg tggcaacgta
    14641 tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga tgaatccttc
    14701 tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc gacgagtgat
    14761 ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta ttccttctct
    14821 ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt taggaacggc
    14881 tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa tcggtgtgat
    14941 tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa aaggaggtcg
    15001 ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct ttttattctt
    15061 atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa cgatgaacag
    15121 ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac gtttgatcat
    15181 tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca cgatcatcgg
    15241 gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga cagcgttgtt
    15301 gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta gtttcttgat
    15361 tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt cccatattgc
    15421 tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa tgaacgattc
    15481 aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca aaaatatcat
    15541 tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta cctattctct
    15601 tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc tctcagtaga
    15661 gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa gtgcgttagt
    15721 tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag aaaaccgtcc
    15781 aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc gatgaaaaaa
    15841 ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct tggtgttcgt
    15901 caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta taactgggga
    15961 gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata caaagtgaat
    16021 tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg tgggacaaac
    16081 tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga aaaactagta
    16141 cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg atttttagac
    16201 ttggattttg atccagataa tcagtattcg attccatatt tttggggaac tttagggatc
    16261 gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga tttgtggaag
    16321 ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat ggggctttct
    16381 ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac agaagcagca
    16441 aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga gatcaaaatg
    16501 tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc tgccgatatg
    16561 atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa tctttggttt
    16621 gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga tttcatcaac
    16681 tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta ttctacacca
    16741 aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca attctatcca
    16801 tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa atacctaggc
    16861 atctataatg acctattctt agaattcaag atgtatagga gataatggca aaactcagta
    16921 agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct atctgagttt
    16981 tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt tattttttct
    17041 accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg cccgagacgg
    17101 gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa actatgcaaa
    17161 tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag ttcaatattt
    17221 gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta gataaattat
    17281 tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa aattggaata
    17341 caaatataga atagacatat tgcagccgat agtttattat gcttttagac acattaaact
    17401 ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga tacgactaac
    17461 agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta tctctcgtca
    17521 gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga aattaccggg
    17581 aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata cggagcaaat
    17641 aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg tcattcaaaa
    17701 atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa atggagccac
    17761 gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc atgagcaaat
    17821 tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg acaatcccga
    17881 actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg ctgaaaagga
    17941 taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg aggtccatcg
    18001 cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa actatcgtcc
    18061 agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg ccacgacgga
    18121 aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta gtgttatgtt
    18181 gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat tattttttca
    18241 aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct tgtctttaat
    18301 gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta aaatcagttg
    18361 tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa atgagatctt
    18421 gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta gtttattgat
    18481 aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg attcgatttt
    18541 ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt cttcgataaa
    18601 gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat caatgagtag
    18661 aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg
    18721 attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca tttccccaag
    18781 ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac ctcggacgac
    18841 ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg acgagaactt
    18901 tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca tcgagctgaa
    18961 gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag tagtgaatca
    19021 attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga
    19081 gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata gtctagtgct
    19141 gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg atttaaaagg
    19201 ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg gcttgaagat
    19261 gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt attcaatcaa
    19321 tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct gtatgtcggg
    19381 tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg atgaatccat
    19441 cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa atgatgaaac
    19501 ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt ccttttcaat
    19561 tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt tggagcatat
    19621 tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc tagtaaaatt
    19681 cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga tcgtgatgag
    19741 tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt taaatggccg
    19801 tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat tcttggctcg
    19861 gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg aattttatta
    19921 tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag tcaatgctta
    19981 tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa gtgtctaaaa
    20041 aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt aacgtgatta
    20101 aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac agaaagaaag
    20161 gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct tcaggctttc
    20221 ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt aatgcttatg
    20281 tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg aacgagcagg
    20341 atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag attaccaaaa
    20401 cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg ccaattctgc
    20461 tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa ctggtaaata
    20521 aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta aatgaagagg
    20581 aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg aacgattaat
    20641 tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt tagtagtagc
    20701 aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg ctgcaccatc
    20761 gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa aagatccgcg
    20821 ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg taaaagttgc
    20881 gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga aaatgacacc
    20941 gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt cagaaattca
    21001 agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta caccggcgat
    21061 ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag gagaaaaatt
    21121 atgtatgatg catttgtagt cgctttggcc gtttttattg gagttgctgg acatgaaatg
    21181 ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg agcgttgatg
    21241 ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt tatgggtgtc
    21301 gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct ggctacagcg
    21361 ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt tccactagca
    21421 gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc gatggctgct
    21481 aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct gcacttcttt
    21541 atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt tttatttgga
    21601 gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa cggattaaca
    21661 gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat gctttggagt
    21721 aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct cgagttacca
    21781 ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca aagagatcta
    21841 caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa tgcaacgctt
    21901 gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct aaagatgaga
    21961 aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg gtgaatccgg
    22021 tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat catttctaca
    22081 aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt tcgacgacca
    22141 ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa aattctgaaa
    22201 aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg ggaccattgg
    22261 ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg ggactagcga
    22321 tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt atgttcaata
    22381 tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta ggctctaaat
    22441 atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct gctagtatct
    22501 tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat accgttttga
    22561 gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat agtattttta
    22621 aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat aaaaaagtcg
    22681 gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca ttacttggaa
    22741 tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga gggaaaagat
    22801 gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg agttaaacga
    22861 cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg atgtaaaggg
    22921 attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt gcgggacgat
    22981 tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag gtgtttgggt
    23041 caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa acgggattgt
    23101 ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag actgtttgcc
    23161 tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg ctcccatact
    23221 ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg aaacacacga
    23281 aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt cggatcagca
    23341 aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg aagtggcaag
    23401 cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt ttcaggaatc
    23461 tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat ttgggcagct
    23521 gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc aaagcttttc
    23581 tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca gctatcctgc
    23641 tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca ccatgtttga
    23701 accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta agtttcctgg
    23761 ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca gtgcgttact
    23821 ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt gtccgcaggt
    23881 tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt caggggaacc
    23941 aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt atcataccgg
    24001 cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg aagccctaga
    24061 acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg aatcaatttg
    24121 taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa tcggtgatga
    24181 ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc aagggttaat
    24241 ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg atggagaaga
    24301 atcagacact acgttgatgc ctttttatca atttttagat aataaagatc atttatatca
    24361 gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg aaatcaaagg
    24421 catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg ttttaatgag
    24481 cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt gtttggcaga
    24541 tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag gagaagtcgt
    24601 gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca gcctaatgat
    24661 cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc ctgttgataa
    24721 tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga ttgaatgtac
    24781 gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa gtgatacgaa
    24841 aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa aaagatgagt
    24901 atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac tcccgaaaat
    24961 ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc aagccgcaaa
    25021 ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga tattactgga
    25081 acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt tggcagtttt
    25141 gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc tgctgttgaa
    25201 agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt gaactcgttg
    25261 ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc tttccaaaca
    25321 tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt tttttatacg
    25381 ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca cgcaaattgg
    25441 catcgggaac gcattaccaa attacaggag gattataagt taattgatcg attaaagggt
    25501 caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta ttggtggggc
    25561 gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt aacctcaaca
    25621 ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga gttcggacgt
    25681 ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc aaatagagat
    25741 gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt tggtgacgac
    25801 gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg ttttaccgaa
    25861 gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt tgagcggcaa
    25921 gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa tactaaacgt
    25981 catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat attcgaaggg
    26041 aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt gtctgtgcag
    26101 tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca actccagttg
    26161 aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg gcaggcatcg
    26221 gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc ggtcatatca
    26281 catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta aagcaatatt
    26341 tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt gaatatgttg
    26401 ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca ggtatcggag
    26461 gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa atggggcata
    26521 tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc caccaagatt
    26581 gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta aaaggccaga
    26641 atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc caatgtgcat
    26701 acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt gtaatgaaac
    26761 aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat ggatatgttg
    26821 aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat gccggaataa
    26881 tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa ttaatgaatg
    26941 tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca aatgaagttg
    27001 gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt gttttcatta
    27061 aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc gctgtatacc
    27121 ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta ctttatgtct
    27181 gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt tgacttcata
    27241 atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt ataaaataaa
    27301 tcgtaattct attatagaga gttgtgatta taaaaagata gcaagaaaaa aaaagaaagt
    27361 ttctatacat tttaaatatt ttattcctat ttctattgct aaa
//
LOCUS       DAZUKT010000034        27231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000034 DAZUKT010000000
VERSION     DAZUKT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.034"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..445)
                     /locus_tag="ACG816_001428"
     CDS             complement(161..445)
                     /locus_tag="ACG816_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920311.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="ACG816_001429"
     CDS             complement(463..1353)
                     /locus_tag="ACG816_001429"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4920312.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="ACG816_001430"
     CDS             complement(1373..1591)
                     /locus_tag="ACG816_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4920313.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="ACG816_001431"
     CDS             complement(1561..3261)
                     /locus_tag="ACG816_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4920314.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="ACG816_001432"
     CDS             3528..4301
                     /locus_tag="ACG816_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920315.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="ACG816_001433"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="ACG816_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4920316.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="ACG816_001434"
     CDS             complement(5125..6096)
                     /locus_tag="ACG816_001434"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4920317.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="ACG816_001435"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="ACG816_001435"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4920318.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="ACG816_001436"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="ACG816_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4920319.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="ACG816_001437"
     CDS             complement(9051..9875)
                     /locus_tag="ACG816_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4920320.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="ACG816_001438"
     CDS             complement(9848..10528)
                     /locus_tag="ACG816_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4920321.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="ACG816_001439"
     CDS             complement(10551..11489)
                     /locus_tag="ACG816_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4920322.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /gene="ispE"
                     /locus_tag="ACG816_001440"
     CDS             complement(11734..12585)
                     /gene="ispE"
                     /locus_tag="ACG816_001440"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4920323.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="ACG816_001441"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="ACG816_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4920324.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="ACG816_001442"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="ACG816_001442"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4920325.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="ACG816_001443"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="ACG816_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4920326.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="ACG816_001444"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="ACG816_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4920327.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="ACG816_001445"
     CDS             complement(19463..20704)
                     /locus_tag="ACG816_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4920328.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="ACG816_001446"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="ACG816_001446"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4920329.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22654)
                     /locus_tag="ACG816_001447"
     CDS             complement(21731..22654)
                     /locus_tag="ACG816_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4920330.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="ACG816_001448"
     CDS             complement(23392..24678)
                     /locus_tag="ACG816_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4920331.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="ACG816_001449"
     CDS             complement(24671..25939)
                     /locus_tag="ACG816_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4920332.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="ACG816_001450"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="ACG816_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4920333.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="ACG816_001451"
     CDS             complement(26711..27157)
                     /locus_tag="ACG816_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4920334.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7741 a         5872 c         4723 g         8895 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa t
//
LOCUS       DAZUKT010000035        26914 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000035 DAZUKT010000000
VERSION     DAZUKT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.035"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..1085
                     /locus_tag="ACG816_001452"
     CDS             81..1085
                     /locus_tag="ACG816_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920335.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1086..2030
                     /locus_tag="ACG816_001453"
     CDS             1086..2030
                     /locus_tag="ACG816_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920336.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2030..2992
                     /gene="opp4B"
                     /locus_tag="ACG816_001454"
     CDS             2030..2992
                     /gene="opp4B"
                     /locus_tag="ACG816_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4920337.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3019..3933
                     /locus_tag="ACG816_001455"
     CDS             3019..3933
                     /locus_tag="ACG816_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920338.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3959..5740
                     /locus_tag="ACG816_001456"
     CDS             3959..5740
                     /locus_tag="ACG816_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4920339.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6088..6774
                     /gene="rnc"
                     /locus_tag="ACG816_001457"
     CDS             6088..6774
                     /gene="rnc"
                     /locus_tag="ACG816_001457"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4920340.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6794..10375
                     /gene="smc"
                     /locus_tag="ACG816_001458"
     CDS             6794..10375
                     /gene="smc"
                     /locus_tag="ACG816_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4920341.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10384..11193
                     /locus_tag="ACG816_001459"
     CDS             10384..11193
                     /locus_tag="ACG816_001459"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4920342.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11204..12202
                     /gene="ftsY"
                     /locus_tag="ACG816_001460"
     CDS             11204..12202
                     /gene="ftsY"
                     /locus_tag="ACG816_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4920343.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12389..14656
                     /gene="gshAB"
                     /locus_tag="ACG816_001461"
     CDS             12389..14656
                     /gene="gshAB"
                     /locus_tag="ACG816_001461"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4920344.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14745..15197)
                     /locus_tag="ACG816_001462"
     CDS             complement(14745..15197)
                     /locus_tag="ACG816_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4920345.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15347..16297
                     /locus_tag="ACG816_001463"
     CDS             15347..16297
                     /locus_tag="ACG816_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4920346.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16290..17249
                     /locus_tag="ACG816_001464"
     CDS             16290..17249
                     /locus_tag="ACG816_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4920347.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17246..18001
                     /locus_tag="ACG816_001465"
     CDS             17246..18001
                     /locus_tag="ACG816_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920348.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18024..18977
                     /locus_tag="ACG816_001466"
     CDS             18024..18977
                     /locus_tag="ACG816_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4920349.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19621..19917)
                     /locus_tag="ACG816_001467"
     CDS             complement(19621..19917)
                     /locus_tag="ACG816_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS4920350.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20272..20628
                     /locus_tag="ACG816_001468"
     CDS             20272..20628
                     /locus_tag="ACG816_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4920351.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20638..21537
                     /locus_tag="ACG816_001469"
     CDS             20638..21537
                     /locus_tag="ACG816_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4920352.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22057..22785
                     /locus_tag="ACG816_001470"
     CDS             22057..22785
                     /locus_tag="ACG816_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4920353.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22930..24957
                     /locus_tag="ACG816_001471"
     CDS             22930..24957
                     /locus_tag="ACG816_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS4920354.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25065..26162
                     /locus_tag="ACG816_001472"
     CDS             25065..26162
                     /locus_tag="ACG816_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4920355.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26469..>26914
                     /locus_tag="ACG816_001473"
     CDS             26469..>26914
                     /locus_tag="ACG816_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920356.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9021 a         4661 c         5685 g         7547 t
ORIGIN      
        1 gttataaaac gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg
       61 aagcagagag gggtattcga ttgtccgata accaattatt agaagttcag aacctacata
      121 caggattccg actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag
      181 acaaaaacga gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta
      241 ctacgattat gggattactt gatcctaata acacaaaaat tacaggtgag attttatata
      301 acgatctgaa tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata
      361 tcggaatgat tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc
      421 agatcaaaga aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg
      481 cactagaatt gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc
      541 cccatgaact atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca
      601 aacctccaat cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac
      661 agattcttga tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca
      721 ctcatgactt aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac
      781 agttcgttga agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac
      841 gctctttact gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg
      901 aaggtgtcgt tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta
      961 ttccatggat accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga
     1021 accacttggt acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg
     1081 tgtagatgac agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg
     1141 gattcttcaa tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg
     1201 aaaaaggaaa gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa
     1261 aagccgttgt gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg
     1321 taacgaagcg aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc
     1381 aagattcaat gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca
     1441 tccgaaattt tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag
     1501 atatcgttgg gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac
     1561 aacgtcaacg tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg
     1621 atgaacctgt atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac
     1681 gaatccagca agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa
     1741 aacacatgtg cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa
     1801 gagaagacat ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc
     1861 cgcaaatcga tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag
     1921 agtatatcca aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta
     1981 cgatcacaac tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac
     2041 gattctgcga cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt
     2101 cttattagcc caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga
     2161 tccggcagtg atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca
     2221 gtacttccgt tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa
     2281 acgctctgtt gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt
     2341 gacagttgtc cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca
     2401 aaattcaatc ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt
     2461 atttatcttc ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac
     2521 aagtggatca caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt
     2581 gaaatatatt ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca
     2641 atatctgcga agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc
     2701 caaaggggta ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc
     2761 gatggcttct caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga
     2821 aaagattttt ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga
     2881 ctataccgtg atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt
     2941 actttcagat atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc
     3001 agaaaggaga atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct
     3061 cctatgggga tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca
     3121 ttaggtttgc tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa
     3181 gacaaagtga tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca
     3241 caaggacaaa agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg
     3301 atcataggtg ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc
     3361 atcggggttg gattagggat catctctggc tactacggag ggatcattga caacatcttg
     3421 atgcggatcg ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta
     3481 tcgattattt ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg
     3541 gtagctaaag cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc
     3601 agtgcctcaa aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg
     3661 aatctaagtt cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc
     3721 gagacaactt tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca
     3781 ttgatcggtt atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc
     3841 gcatcaatcc ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag
     3901 cgttctgcag atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat
     3961 gaagaaaacg atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg
     4021 gggcggaggt acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga
     4081 attaactttt ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga
     4141 agctgcggtc gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga
     4201 ttcatatgat gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga
     4261 tttcagaatc acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc
     4321 gacaatcaaa gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga
     4381 tgtgattttc ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga
     4441 cactttccgt aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc
     4501 cggaatcaaa aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag
     4561 catgcttcaa tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg
     4621 tgtagcaatc aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg
     4681 cccttattac atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca
     4741 ctactggaat ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag
     4801 tgcaactgaa gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac
     4861 ttacgatacc tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta
     4921 tacttatcta ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa
     4981 tccagattca aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa
     5041 taatgcagta ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc
     5101 tccagtattc cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa
     5161 agcgaaaaaa ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga
     5221 agacaaagat ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc
     5281 gcaaccattg gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata
     5341 cacaacaggt cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc
     5401 tgaaattgat gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact
     5461 atggggacca aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt
     5521 gatcaacgat atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca
     5581 aaaatggcaa caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga
     5641 agtattgcca gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc
     5701 atgggcaaat gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa
     5761 agggcgatgt cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt
     5821 caaggcgctg atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat
     5881 taagtcaaat ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc
     5941 gaaactaaac gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt
     6001 accttttgac cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa
     6061 aaaatatcca taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa
     6121 aactataaca tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc
     6181 tatgtgaatg agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga
     6241 gatgccgttt tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca
     6301 gaaggaaaat tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt
     6361 gccaaagaat gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga
     6421 ggacgtacgc gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat
     6481 cttgaccagg gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc
     6541 aaagcaggtg ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa
     6601 aaatcaggtg atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa
     6661 cgagtatttt ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa
     6721 tcgaaaaaat tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa
     6781 gtgagggaaa gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga
     6841 caagacagtc attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg
     6901 aaaaagcaat attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct
     6961 gcgaggcgga aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa
     7021 tattgcagaa gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa
     7081 tgagatcagt gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa
     7141 acaaagctgc agattaaaag atatccaaga attgttctta gattctggat taggaaaaga
     7201 atcgttctca atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga
     7261 ccgtcgtggg atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa
     7321 agcagaacag aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca
     7381 cgaattggaa gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt
     7441 acggctaaaa gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac
     7501 agcaaaaaaa gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg
     7561 caaactatct gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa
     7621 tgcgcaagcc gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa
     7681 actgaagcag acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa
     7741 aagctcgcaa gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga
     7801 aaagcttcaa gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc
     7861 tgaagcaaat ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga
     7921 attattggca gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg
     7981 aaacgaactg aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac
     8041 gttagccaag caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga
     8101 attgacacaa aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga
     8161 aatgatccaa caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat
     8221 gtatcaatta atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat
     8281 ccaagaaaac tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca
     8341 gctttcgggt attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt
     8401 agctatcgaa gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga
     8461 tgctcggcaa gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc
     8521 actgacgaca atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt
     8581 agaagggttt atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt
     8641 ggttcaaaac ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat
     8701 cgcgcaaacg atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc
     8761 cggaggatcc atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca
     8821 aaatcaagaa ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc
     8881 acaagaaaaa aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga
     8941 agttttgcgt acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct
     9001 acaaaacatc ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga
     9061 gacacgtgaa ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga
     9121 caaacaaaca gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag
     9181 tcaagaaagc gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga
     9241 acaagcagat cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg
     9301 tgcccgagtc caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc
     9361 gacattgaca gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat
     9421 taacgaatta tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca
     9481 gcgagataga aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg
     9541 tgagcaaaaa gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga
     9601 aatgatttta gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa
     9661 agcttcccaa gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc
     9721 cttaaaagaa cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga
     9781 acaagtcagt gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa
     9841 aaaccaattg tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga
     9901 agtatttgaa gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg
     9961 taaagctgaa ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga
    10021 agtacaacct cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc
    10081 acttacagcc atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat
    10141 cttagatgag gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt
    10201 gagcgatttc caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt
    10261 cgcagcagat gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc
    10321 tgtacggatg gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg
    10381 aaaatgatca aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg
    10441 atttcagatg agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt
    10501 ctatgtacag gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta
    10561 gatgaaggtg actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt
    10621 gaaatcatgg aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct
    10681 cagcaattgg atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt
    10741 gccccaagga agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg
    10801 gatataagcg atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa
    10861 gaagaattag atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc
    10921 atcaaaacaa gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt
    10981 atttctttat tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat
    11041 gaggaaaatg atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct
    11101 gtgcctttcg tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta
    11161 gccaaagtga tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa
    11221 aatcaagcgt gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta
    11281 cgacaagggt ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc
    11341 caattttcga acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc
    11401 agatgtaggt tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact
    11461 cagaaatgcg aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct
    11521 ctatgaagcg gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac
    11581 agttattttg tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc
    11641 ccatcagttc aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc
    11701 tggagcaatc gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg
    11761 caatgcagga ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga
    11821 gcaggcagat gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat
    11881 gaacgaatta gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga
    11941 aacacttctt gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt
    12001 caaagaaaca acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg
    12061 cggtatcgta cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg
    12121 tgaaggaatc gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa
    12181 aggattacta aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca
    12241 gctccttgtt tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata
    12301 cttatttgta agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat
    12361 tagattcata tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt
    12421 caatgcgcgc ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt
    12481 tgatcttgca ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta
    12541 tcacccctat atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac
    12601 cgattctatt cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc
    12661 tataccgaaa gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga
    12721 cgaagagatc attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt
    12781 agcaaaaaaa tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt
    12841 tggcgatgag ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga
    12901 attcaaaaca gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat
    12961 cacttattta ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca
    13021 tccgcaagaa cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa
    13081 tgtgaaagtt tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga
    13141 agaagggaaa ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa
    13201 gaaagtcgca gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt
    13261 gaatccttat gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct
    13321 gatgtacatg ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac
    13381 gactcgaaat aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga
    13441 aggtagagaa attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc
    13501 tctttattta gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc
    13561 gaaactctat caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca
    13621 tcaatattat aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga
    13681 actttctact cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt
    13741 agatgagtcg gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa
    13801 tgcaaacatg acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt
    13861 aacgaaaaaa gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc
    13921 catggaagaa gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc
    13981 taaatcaacc aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga
    14041 ttatcaagca ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt
    14101 tatgccggga acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt
    14161 gcgagtccct gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga
    14221 aaaaaatagt gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt
    14281 aggtgaatta gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca
    14341 agccaatcaa attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat
    14401 cgatatgact gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc
    14461 actaggagct aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac
    14521 aactgataaa aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca
    14581 tgtttatcct tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata
    14641 tcctgaagta ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga
    14701 tcaaaagctt tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc
    14761 cagaagcttt ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg
    14821 tatttttttt cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat
    14881 ccgaattcct caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt
    14941 ttcgttgcta gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt
    15001 atggaagcat ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg
    15061 atcatttcag caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta
    15121 tgctgttctt cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt
    15181 acatcatctt gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata
    15241 gcatattccc aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca
    15301 cttaaaataa gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg
    15361 actagtagca ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt
    15421 acaattgtca gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg
    15481 tttgccacga acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat
    15541 catgcaacat ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag
    15601 tgcgcgttta ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg
    15661 atccttgaca gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg
    15721 attgttcctc cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat
    15781 cattggttca gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc
    15841 atggcttcaa gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt
    15901 tgttccattg ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg
    15961 cgaatcgttt gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg
    16021 cttagtatca ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg
    16081 agtgatcatt ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg
    16141 cgtaactgct ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt
    16201 gatttttcct tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt
    16261 cctttatctt ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg
    16321 gtatgcttgt tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa
    16381 actggcattt tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc
    16441 tagctgtcag cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc
    16501 cttcagtatt gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta
    16561 tcggaggagt gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc
    16621 tgatggtcgg gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc
    16681 tttttttatt actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct
    16741 ttttacaagt catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa
    16801 gctttggcaa tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct
    16861 ggtacctgtt atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag
    16921 cagtaaacct cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc
    16981 ttttgacagg gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg
    17041 caaatatcag ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct
    17101 ttctggtagc tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct
    17161 ggaatacaac agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat
    17221 tgattcgcga aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca
    17281 tggaaaatca gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt
    17341 tatcggtcca aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc
    17401 aaaagataca ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga
    17461 attagccaaa aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt
    17521 gaaagaatta gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga
    17581 cgaggaaatc gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc
    17641 gattgacact ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca
    17701 agacacggat tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt
    17761 tcaaatcatg cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt
    17821 tcttcatgat atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg
    17881 acagttattt acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt
    17941 atacgaaatg gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta
    18001 aaactaacat taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat
    18061 catttggttt agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa
    18121 gcagcacaca aactgaaact acattgacga taacagatag taatggagac caaatcgagg
    18181 tgccaaataa tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg
    18241 ctcttggtgt tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt
    18301 catcctataa aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta
    18361 atcagcttca gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc
    18421 taagtcaaat tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt
    18481 tccagcaaaa tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc
    18541 aattagagga attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca
    18601 aaaaagcact agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta
    18661 gattcggttt catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat
    18721 ccactcacgg tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat
    18781 ttgtagtaga cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca
    18841 atgaactggt agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag
    18901 atgtctggta tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga
    18961 atcaagcatt taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa
    19021 tgtttcttac attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc
    19081 gtaaaatcgt attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa
    19141 cgtgaattca ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag
    19201 tgtgatccat tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc
    19261 gaaacttaga taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac
    19321 gatgttccat ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta
    19381 ttttctgtcc agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa
    19441 cgatgttcca tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct
    19501 attttctgtc cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa
    19561 acaatgttca atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat
    19621 ttaggcatgt gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc
    19681 ggcgggaagc ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat
    19741 tttatactcg cttaatatca atccaaaact attcgttttt tttgaagcta attttttata
    19801 aatatccaat gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga
    19861 accctcgata ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt
    19921 gattcctcca aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat
    19981 ccgtgaacaa aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt
    20041 attttttgtt atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct
    20101 ttttccgttc aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa
    20161 atcataataa acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat
    20221 tagttgcatc tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta
    20281 gaagtgacaa aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt
    20341 ttattagata tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg
    20401 gatacggcat ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat
    20461 tctgtagtgg ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac
    20521 atggatgatc catccacact tgctttagaa ccaacttata atagtttaca attaaaagga
    20581 ccaagcggct tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg
    20641 ggaaaattgg cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag
    20701 ctgatgcagc tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga
    20761 gatacggcta ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga
    20821 ttatctacga catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg
    20881 atggaccatt ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag
    20941 accgttcttt tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac
    21001 gaggcaatcg tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa
    21061 acagatctcg aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg
    21121 gaagcaaaaa aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc
    21181 atgtatcaat ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa
    21241 tcattcggag aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg
    21301 catcgtcgct ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt
    21361 tattattatg aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc
    21421 tatgatccta tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat
    21481 caaacacaac atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac
    21541 agcattagta atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta
    21601 gatccattta tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt
    21661 gatcggatga tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg
    21721 ataatcacaa aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga
    21781 agtctaggca gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa
    21841 ggctatgaca gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat
    21901 caaatcgggc tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg
    21961 ttcttttatt tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg
    22021 atatgtttag taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca
    22081 gcagcaatga tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta
    22141 tacccaaagg aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta
    22201 actcctcctg ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct
    22261 ccaagccctg gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg
    22321 actcaaaaaa tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat
    22381 gggtctagaa aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg
    22441 aaattaagtg tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga
    22501 gcacagctga aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg
    22561 caaacggtga attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat
    22621 gctaaatcag gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa
    22681 aatcaagatg cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat
    22741 tccactaaat tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac
    22801 tgttaaaaat aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa
    22861 ggctaggcta aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga
    22921 aaagatatat tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat
    22981 atctttccga tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga
    23041 gacgaagaaa gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa
    23101 gaaagcagtg aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct
    23161 gagggttcgg acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac
    23221 gatcccaata ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc
    23281 atattggaaa atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct
    23341 atttggtcaa ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac
    23401 ttaggaaatc aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct
    23461 agaatggcta gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat
    23521 tctactcgtt ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat
    23581 aaaaatagtg gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat
    23641 ggacatgtcg cttttgtcac gcctaaacca aataataata attattctgg agagcatact
    23701 ggcgtgacgg tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa
    23761 tgggacgcgg agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg
    23821 attatacctg atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct
    23881 tcaacaggta ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc
    23941 acatcaacag ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga
    24001 ggaaacgata agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat
    24061 aagaaaatgc agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg
    24121 atcaatggaa atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta
    24181 aatctgacag attattttat tgctggagat acgttaataa tcgaattgga gacaactttg
    24241 caggaagtaa ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat
    24301 ttgattgagc aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc
    24361 catgtttatt ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact
    24421 gggaaaatag gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac
    24481 tcggattcag cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat
    24541 tacagaaaag gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat
    24601 aaaatatcca gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata
    24661 gatgaacggg cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat
    24721 ggagattttg agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata
    24781 gggacatccg ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct
    24841 cttttagatt ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga
    24901 atcggaacct ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag
    24961 gaagagaaga atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct
    25021 tgtgtcccac agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga
    25081 ttttcttgag accaaaacaa caattattta taacactcct agttatttta ggggcacttt
    25141 ttaacaatat atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag
    25201 agaatcaaat cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag
    25261 tagaagtaaa agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg
    25321 tgaatagcgc aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa
    25381 aagacatgag cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc
    25441 taaaacctaa aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg
    25501 atggtgtatt ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag
    25561 aaactaagga tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga
    25621 tgcaacagac actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc
    25681 aaatcaatgc aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca
    25741 atcaagtagt tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag
    25801 aggaaaaaga aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa
    25861 atggtcaacc gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa
    25921 atgaaaatgg aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat
    25981 gggtatttga aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg
    26041 atgttacgat taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat
    26101 tgtttatttt attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt
    26161 aaggttaaga taacaaatag taaattttta ttattgtagg atggttgggc tctttttata
    26221 acaatataaa atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta
    26281 ataacaggtt tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag
    26341 acaggacgat gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa
    26401 atcagtttta tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct
    26461 gatgactaat gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac
    26521 ttaaatattt agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt
    26581 tgtcgctatc gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa
    26641 agttcgatct agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa
    26701 ttaatggaaa atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta
    26761 tgaaaatggt attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac
    26821 agcatcacgt gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa
    26881 aaaaatatca aatttttttt gaaaagagaa agtt
//
LOCUS       DAZUKT010000036        26769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000036 DAZUKT010000000
VERSION     DAZUKT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.036"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1959)
                     /locus_tag="ACG816_001474"
     CDS             complement(28..1959)
                     /locus_tag="ACG816_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4920357.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1977..2891)
                     /gene="pfkB"
                     /locus_tag="ACG816_001475"
     CDS             complement(1977..2891)
                     /gene="pfkB"
                     /locus_tag="ACG816_001475"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4920358.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2888..3640)
                     /locus_tag="ACG816_001476"
     CDS             complement(2888..3640)
                     /locus_tag="ACG816_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4920359.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3800..4009)
                     /locus_tag="ACG816_001477"
     CDS             complement(3800..4009)
                     /locus_tag="ACG816_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920360.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4020..5347)
                     /locus_tag="ACG816_001478"
                     /pseudo
     CDS             complement(4020..5347)
                     /locus_tag="ACG816_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5483..5638
                     /locus_tag="ACG816_001479"
     CDS             5483..5638
                     /locus_tag="ACG816_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4920361.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5712..6299)
                     /gene="yihA"
                     /locus_tag="ACG816_001480"
     CDS             complement(5712..6299)
                     /gene="yihA"
                     /locus_tag="ACG816_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4920362.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6382..7632)
                     /gene="clpX"
                     /locus_tag="ACG816_001481"
     CDS             complement(6382..7632)
                     /gene="clpX"
                     /locus_tag="ACG816_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4920363.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7879..9168)
                     /gene="tig"
                     /locus_tag="ACG816_001482"
     CDS             complement(7879..9168)
                     /gene="tig"
                     /locus_tag="ACG816_001482"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4920364.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9322..10254)
                     /locus_tag="ACG816_001483"
     CDS             complement(9322..10254)
                     /locus_tag="ACG816_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920365.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10411..11070)
                     /locus_tag="ACG816_001484"
     CDS             complement(10411..11070)
                     /locus_tag="ACG816_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4920366.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11073..12098)
                     /gene="sppA"
                     /locus_tag="ACG816_001485"
     CDS             complement(11073..12098)
                     /gene="sppA"
                     /locus_tag="ACG816_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4920367.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12111..12401)
                     /locus_tag="ACG816_001486"
     CDS             complement(12111..12401)
                     /locus_tag="ACG816_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920368.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12810..14615
                     /gene="glmS"
                     /locus_tag="ACG816_001487"
     CDS             12810..14615
                     /gene="glmS"
                     /locus_tag="ACG816_001487"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4920369.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14687..16042)
                     /gene="glmM"
                     /locus_tag="ACG816_001488"
     CDS             complement(14687..16042)
                     /gene="glmM"
                     /locus_tag="ACG816_001488"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4920370.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16066..17235)
                     /locus_tag="ACG816_001489"
     CDS             complement(16066..17235)
                     /locus_tag="ACG816_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4920371.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17232..18119)
                     /gene="cdaA"
                     /locus_tag="ACG816_001490"
     CDS             complement(17232..18119)
                     /gene="cdaA"
                     /locus_tag="ACG816_001490"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4920372.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18308..22000)
                     /gene="nifJ"
                     /locus_tag="ACG816_001491"
     CDS             complement(18308..22000)
                     /gene="nifJ"
                     /locus_tag="ACG816_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4920373.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22199..22720
                     /locus_tag="ACG816_001492"
     CDS             22199..22720
                     /locus_tag="ACG816_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4920374.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23419..23976)
                     /locus_tag="ACG816_001493"
     CDS             complement(23419..23976)
                     /locus_tag="ACG816_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920375.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(24155..24766)
                     /locus_tag="ACG816_001494"
     CDS             complement(24155..24766)
                     /locus_tag="ACG816_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920376.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(24879..25136)
                     /locus_tag="ACG816_001495"
     CDS             complement(24879..25136)
                     /locus_tag="ACG816_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920377.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(25733..26011)
                     /locus_tag="ACG816_001496"
     CDS             complement(25733..26011)
                     /locus_tag="ACG816_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920378.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(26045..26296)
                     /locus_tag="ACG816_001497"
     CDS             complement(26045..26296)
                     /locus_tag="ACG816_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920379.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         7559 a         5767 c         4585 g         8858 t
ORIGIN      
        1 gaaaacccag cctcttcttt tttatttcta tgcttctgct actgcaggag cttttctcaa
       61 taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac
      121 cgggtgactc agtaagaata caacgaaaat cccgccgtga ggagccagta atttgattcc
      181 gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg
      241 aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac
      301 gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt
      361 agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac
      421 acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt
      481 gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc
      541 agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat
      601 agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa
      661 gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa
      721 tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc
      781 tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact
      841 tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg
      901 gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac
      961 gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa
     1021 aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc
     1081 atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt
     1141 accactaagg gctagattga tcaactcttc tggtttacgg ataccatcac tgacaggacg
     1201 attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat
     1261 tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc
     1321 gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca aagcatcttc
     1381 tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct
     1441 ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc
     1501 tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt
     1561 taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa
     1621 atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc
     1681 gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt
     1741 aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg
     1801 tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc
     1861 aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat
     1921 gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa
     1981 tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag
     2041 tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga
     2101 tctttcgtac ggctgtaagc cccaataaag ccagcaatca ttgaatcccc tgcacctact
     2161 gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc
     2221 aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc tagtaatttt
     2281 ttcccatacg gaatgatttc ttctacagat tggaaggttg tgttgtaaag ctcagctaat
     2341 tcgtgattgt ttggtttgat caacaacggt ttcttaacaa gtgcattttt taggtcatca
     2401 cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga
     2461 taaaatcctt ttcgcaggct agctggcgcg cttcctgaca atacgacgat atctccttgt
     2521 cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggcct
     2581 aagccattga tctcagtttc agtgtctgat tttagtttga tattgattct tgtatcttct
     2641 tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg
     2701 ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata
     2761 cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa
     2821 tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact
     2881 gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt
     2941 ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg
     3001 acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg
     3061 ctaaacgttt aacagctgcc tcttctggat ccggagtcgt gaatccttgt tcaagatggg
     3121 caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat
     3181 tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa
     3241 gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt
     3301 ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat
     3361 cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt
     3421 tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc
     3481 catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg
     3541 attcagaagc attcaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa
     3601 gcaagtctag gattttttgt ctgcgttcct ctgtaagcat tttcacgatc acctcaatac
     3661 aaataatagc atattttttt tctactagca atataaatca gtcaaaaacg ttcataaaaa
     3721 ttataaatca gtcaaaaaaa aaataaaaaa ccagtcagaa agactggttt tttatttttt
     3781 tgtctatcat caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa
     3841 cagaagcagg ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca
     3901 tgaccaaaaa accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg
     3961 ataataatga tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct
     4021 catatttttt gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc
     4081 agatgctgct cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt
     4141 ttgaaggtaa catcagagag tctatgtttg atttccttta acggtaattc tgacagggat
     4201 tctgaaggaa catagattct taaggataaa agatactctt gatatttttc atctgttacc
     4261 tctacttcta gatattccct aatataataa caataagctt cgattccatt atccaaagca
     4321 acagtcggat cgataacttt atagcacatc atgcgtgatc gattctcgtc aacccaagga
     4381 aaatacgcct gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttgg
     4441 atatcataac tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt
     4501 gcattctgat actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc
     4561 ggtttcaata aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta
     4621 aaataggatt gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc
     4681 tggatacata tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga
     4741 aaaatcggtt tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa
     4801 gtgaaacata aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact
     4861 acttccccgt tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac
     4921 aatagccgtt gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata
     4981 taggcgaaca ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc
     5041 ttttctttga aaacagagat agacaataca ttttttatca ataattgatt atcttccaag
     5101 cgaaaactac attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat
     5161 gctagcatta ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa
     5221 gagcggataa atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc
     5281 tgtttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
     5341 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
     5401 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
     5461 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
     5521 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
     5581 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
     5641 taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt
     5701 ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc
     5761 tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt
     5821 ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca
     5881 acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga
     5941 gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt
     6001 aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac
     6061 ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc
     6121 tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg
     6181 ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta
     6241 tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg
     6301 gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac
     6361 ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt
     6421 tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt
     6481 tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg
     6541 agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa
     6601 ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt
     6661 cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac
     6721 aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat
     6781 atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt
     6841 cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat
     6901 gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc
     6961 ctgaggtggc acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc
     7021 ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat
     7081 ctcatcaata tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag
     7141 caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt
     7201 cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt
     7261 tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc
     7321 tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa
     7381 cgagcgtttt gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag
     7441 tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat
     7501 ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt
     7561 tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg
     7621 attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga
     7681 ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg
     7741 atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa
     7801 aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa
     7861 tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat
     7921 tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg
     7981 ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc
     8041 tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg
     8101 catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg
     8161 atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga
     8221 acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac
     8281 agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac
     8341 taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc
     8401 ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac
     8461 tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac
     8521 atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat
     8581 gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc
     8641 gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc
     8701 ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc
     8761 agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt
     8821 tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct
     8881 ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc
     8941 ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat
     9001 acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt
     9061 tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa
     9121 ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc
     9181 gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac
     9241 taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt
     9301 tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct
     9361 tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca
     9421 cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg
     9481 ataaacaatt gcagattgca acgtgaattc ttcttttaac acggaactaa gcagcgcatc
     9541 ttcattcccg taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg
     9601 ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac
     9661 aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg
     9721 gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg
     9781 ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga
     9841 tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa
     9901 ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa
     9961 ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa
    10021 ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt
    10081 ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa
    10141 ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg
    10201 ttgtgccact cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg
    10261 tcctcctctt gctcatattc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca
    10321 attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg
    10381 ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt tgaacgcttt
    10441 gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt
    10501 ctgattaaag actgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg
    10561 gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat
    10621 gactcgaata ccaaagatca ttttcccaat cgtttggcca tgattgaatt tcgtcaataa
    10681 aataaaatac gctagataaa taagtaaggc tgtgaatccg tagagactca gataagaatc
    10741 tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc
    10801 gatacacaaa agatcgacca agaaggcaaa cattcggata aaaaaacctg catagaaata
    10861 cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt
    10921 acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac
    10981 cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg
    11041 ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct
    11101 tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa
    11161 ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat
    11221 ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt tcgtatagct
    11281 tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca
    11341 ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc
    11401 tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct
    11461 tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta
    11521 actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg
    11581 atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca
    11641 gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca
    11701 tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca
    11761 ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc
    11821 agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct
    11881 cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct
    11941 tccaacacat ttggagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt
    12001 tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct
    12061 gcagcaatca gaactgctgc ccaacgtctt ttattcattc tttctccttt ctaatacaag
    12121 ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct
    12181 tcccgcgcat acccggaaaa cgactctttt gttaattcat cttctaccgt aaactcacga
    12241 gcttgagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct
    12301 aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt
    12361 tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc
    12421 gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt
    12481 tgacataaaa aggacatttg acaaagaatt tgatatatac caatttattt tttattcttt
    12541 ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca
    12601 aaaaggagga tagttatacc ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg
    12661 agagggttta tcgaaagatt cggcggatgg ccctagggac tgcactctac agaaaatcca
    12721 caaaaaatgt ctgcaaagat aaaacaaaag gattttcctg cagctggaag cattcatacg
    12781 aattcattat agaaagtagg atgtttttta tgtgcggaat tgttggaatg attggattaa
    12841 aaaatgtaac accaggattg attgacggtc tagaaaaatt agaataccgt ggttatgatt
    12901 cagctggtat ctttgtctca gatggcacaa ctgattatct ggtcaaagca caaggcagaa
    12961 ttcaaaattt gaaaaacaaa ataaatattg atactacagg aaatataggt attggtcata
    13021 cacgatgggc aactcacggg caaccttctg aagaaaacgc ccatccacat acttcccaaa
    13081 gtggacgctt cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg
    13141 cttatttagc aaatgaccac tttattggtg agaccgatac tgagattatc gctcatctaa
    13201 ttgaaacttt cgcgaaggat actacaacgc aagaggcgtt tttaaaagct ttgcgggtca
    13261 tcaaaggttc ttacgctttc gctttgattg atcggacagc acctgatgtt atttacgtcg
    13321 ctaaaaataa aagtccacta ttaatcggac tcggcgatgg attcaatgtg attgccagcg
    13381 atgcaatggc tatgcttgct catacgaagg aattcgttga gattgaagat gaagagatgg
    13441 ttaccgtcac atcagaaaaa gttattattc aaaattttgc aggtgacatc gtagaacgtt
    13501 cttcattcga agcacaagta gatgcttcag atatcgagaa aggtacctat cctttttata
    13561 tgctgaaaga aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa
    13621 cagaagacaa tcgtgtctct cttgatcaaa agctggtcga tacattgtct gatagcgatc
    13681 gaatctatat cgttgcttgc ggaactagtt accatgcggg gttagcaggt aaacagactt
    13741 tggaaaaatt gacacagatt cctgttgagg ttcatttagc cagtgaattt ggctataaca
    13801 cacctcttct ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc gaaacagcag
    13861 acagccgcca agtgctggta aaaatcaacc gtctcggcta cccttcactt acgattacaa
    13921 atgttgctgg ttctactctt tcgcgggaag catcctttac actgctattg catgctggac
    13981 ccgaaatcgc agtagcatca acgaaagctt atactgcaca aatagctgta ctaactttat
    14041 tagccaaagc tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc gatatcactc
    14101 atgaattgag tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa gattatttag
    14161 aagagttggc agaagtatat ctcagcgata caagaaatgc cttttacatt ggacgtggtg
    14221 ctgactacaa cgtttctctt gaagctgctt tgaaattgaa agaaatcagt tatatccagg
    14281 cagaaggttt tgccgctggc gaattgaaac atggaacgat tgctttgatc gaagaaggaa
    14341 caccagtgat cggaatcatc agtgaagaag tgactggtgc gcatactcga ggaaacctga
    14401 aagaagtcga aagccgtggc gctaaaacac tagttattgt ctcagaggga ttagaaaagg
    14461 aaagtgacca gctggtgcta ccaactgtcc acccttatct aactactcta accacagtcg
    14521 taccgactca attacttgca tattacgcaa cgctattacg gggattcgac gttgacaaac
    14581 cacgtaattt agcgaaatca gtgacagtag aataataaaa gaacaaggac cagtgtcaga
    14641 catctgacac tggtccttgt tcttttattt tttatgaaaa tatctcttaa tccagtccga
    14701 tttcttcttt gactactgcc gcgattttat cgacatagta attaactttc tcatcagtag
    14761 gcgcttctgc catcactcga agtaatggtt ctgtaccgga aggacgaact aggattcttc
    14821 cttctgatcc catttcagct tctgcctctt caatcacttt tttgattgcc ggaacatcca
    14881 tcgcaccgtt tttatcactt acgcggatat tcactaattt ttgcggatag attgttacct
    14941 ctgctgcaag ctctgataat ttcttacctg tctgtttcat gatattcagt aactgaattc
    15001 ctgaaagcat cccatctcca gttgtgttaa aatctaagaa aatcatgtgc ccagactgct
    15061 cgccgccaaa gttataatcg tttttgcgca tttcttctac aacgtaacga tcaccaactt
    15121 gtgtaacgac atctttcaaa ccgattcctt ccaccgcttt gtggaatcct aagttactca
    15181 tgacagtcgt aacgatcgta tcttttttca atcgattttt ttctgccaag tatttagcac
    15241 agataaacat gattttatca ccgtcgacaa tgtttccaag ttcatccacc gcaataatac
    15301 gatcgccatc gccatcgaaa gctagtccag catctgctcc tttttcaacg accatctcag
    15361 ctaaacgttc tggatgggta gagcctacgc cgtcattgat atttaacccg tctggatttg
    15421 tgcccattgt ataaaaatct gtttcaagat cggcaaacaa acgattaaca cttgttgaag
    15481 ttgctccgtt tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt
    15541 gtacaaggaa ttgagaatat ttcaataagc cttctggaaa ttcttctaca gttcccaatc
    15601 cttcagctga tggacgaggc aattcatcca cttcagcatc taataatgct tcaatttcta
    15661 gctcttggtc atctactagt ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt
    15721 gtgctggatt atgagaagca gaaatcatga cccctgcgct tgctttttgc aaacgtgtca
    15781 gataagcaac acctggtgta gagatcacac ctagctggaa cacttcgata cccaccgata
    15841 aaagtcctga aatcagcgct tcttctaaca tttgacctga gattcttgta tcccgcccta
    15901 ctaacacgcg tgggcgtttt ccttcttctt catgctggct taggacataa cctccgcacc
    15961 gtcccagctt aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt
    16021 ctgttccaaa atatttaccc atagttgtaa aattactcct ttaacttaat tttctgagga
    16081 atttgttctg ctcatttgtg ttgattcgct tgtttctgat gtttcttctg tacttgtggt
    16141 cgagctgctt gtcccagatt ccgaactgga agaaatggtt gttgtcgatg gttccgttcc
    16201 tgaatcagca gatgaaatac ttctactcgt acttgtattt tcttgtgtcc gactgctcgt
    16261 cgaagcagag ctactgcttg acgtttgcaa cacgggtgta atctccacac taactgattc
    16321 gggcttgata cttaacccct cttctactgg tatttcaatt ttacgagttg ttcgatgatt
    16381 gatacctgag acattgatcg gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct
    16441 agagccgctt acttctgctt ccagttttga aaaactgaaa tcatagcttt ctaccccggc
    16501 agctggtgtt ccttgctgta tcccataaag tcgtaccgac ttgcttggct tgatcgaatc
    16561 agctctcact gttacttgca ctggatcaga gacgatactt aatacttccc ctgctgcatt
    16621 cagcgcctgt attgggacct cactattaat cccatcatct gtgaccatcg aagcgtccac
    16681 atcagctact actcgtttga tttctgccaa tgttttgtca cctgtagtga tagagacttt
    16741 tttaggacta acggaagtat catcaagttc atatccagat ggtgtgaacg ttgaaggaat
    16801 caacgtttcc acatcgaaat cttttattac ctttttctca accgttactg taatagtcga
    16861 tggttcgatc tttgctgtga cagcactgct caaattttga atttttagcg gaacttcatg
    16921 tgttccttcg cccagcttag tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt
    16981 tgcctctgca ttcaactgaa ctcggtttgc actggaaagt ttgacagtca ctgcattttc
    17041 atatccatgg atatagtagc gctttgaatc ataagaaatc ttgatcggca cccccgtaac
    17101 tgtttgatta tacgcttcat ttccagaaag cgtatttgga acggactggc catttgcatt
    17161 gaagtacagc gcaatactga acagcaatgc aataaaactg taaagaatgc ttgtccgttt
    17221 ctcttttgag atcatttgcg atcccctccc ttagtcatac cgtctaggaa agattgaatc
    17281 acgtttttct tgttttttgt tttctcttca gcaattagtt ctctacgcaa gattgccaaa
    17341 tattcttgct gggataagcc agccatcagt tcattatcta acgtgatgct gacgtccccg
    17401 gtttcttcag agacaacgat ggtaatcgcg tcactgactt cgcttatacc gacagctgcc
    17461 cggtgtcgag tcccgaattc tttagggatc aacatacttt ctgatagagg aagataggcg
    17521 cttgctactg cgatctttcc ttcttttaca ataactgcac cgtcatgcag cggtgtatta
    17581 ggaataaaga tattgatcag cagctcccca gtgatatctg catctaaagc aatccctgtc
    17641 tcaatatact catctaatcc agtatgacgt tcgatagtga tcaacgcacc gatttttcgc
    17701 ttggacatat attggatggc tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt
    17761 tcgctttttg attgtttgaa aagcgaactg cgtccaagat gttccagtcc tctccggatt
    17821 tccggctgaa aaatcacgat agcagctatc accccgtacg taatgacttg gttcatcagc
    17881 caagacaagg tgtgcagacc aatgatttcc gctacgattc ggataacaat aaaaatagct
    17941 acacctttca gaagctgaat cgcttttgtg cctcgcagca gttggatcag tttgtataca
    18001 agataccaga caaccagaat atcaagtatg ttcaccaaaa aattcagaga gaacaagtca
    18061 ccggaaaaca tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg aaatgacata
    18121 aaaagcctct cttctctttt gagatttcct ctctagtata tcatatgttt taattttaga
    18181 ggctgatatg ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa attgcaagta
    18241 tgcgttttat ttttctctgc aataaatatt tttaaacttc agagaattat tcattatatt
    18301 ttttctgtta tttttccttt ttttcttttg tagcttgttc cattttttta cgtcgttctg
    18361 ctctggcagc tcttctagca gctcttcttt cttctgcttc aaggtccgtc gttttttctt
    18421 ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt
    18481 ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtcg tggacagtct
    18541 tttcaaataa agcttttgca agatttggat ttgcgttatc tagcgaggaa aagcgaactt
    18601 gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg
    18661 ggttcttgtt tttttcgacc attgtagggt tatatcgata taatgaccaa taaccagaag
    18721 aaacagcttc ttttgcttct ttaagcgatt ctttcattcc gccaagcaag ccatgtgtga
    18781 tacaaggcgt ataggcaata atcagagaag gaccaggata cttttctgct tcttcaaatg
    18841 ccttgatcgt ttgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat
    18901 atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt
    18961 tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt
    19021 cgttatccaa aacaaggata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc
    19081 caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga
    19141 atagatcctt tctcttgtat atatcctcca gttcagggaa aattgatcgt tcgctttcga
    19201 tcgcagcttg cagttttgct gctctttgct gcgttccttc actctcaaac atgtgtacta
    19261 accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg
    19321 aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag
    19381 cattatcttc caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat
    19441 aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca
    19501 tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg
    19561 agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct
    19621 ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt
    19681 gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct
    19741 ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc
    19801 cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt
    19861 catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca
    19921 tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag
    19981 aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag
    20041 agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt
    20101 tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt
    20161 gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg
    20221 atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag
    20281 gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca
    20341 aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag
    20401 ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag
    20461 tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt
    20521 aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg
    20581 gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat
    20641 aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat
    20701 ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt
    20761 ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc
    20821 ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg
    20881 agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg
    20941 ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg
    21001 cccctggttc tttacttcga tcaagaacag caattgtttt tactgtatga ggcaactttt
    21061 gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc
    21121 gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag
    21181 aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc
    21241 ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat
    21301 aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa
    21361 cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga
    21421 tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac
    21481 ggaatccatc aaagaaattc aaaaaaggga cttctccttc aatagaagca agatgggcaa
    21541 caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt
    21601 gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg
    21661 cccgactagc aacgtggaaa acacttggca gtagttcccc agcaatcttg tacatattcg
    21721 gtatcatcaa aagcagccct tgtgaagcgg tatatgtagt tgtcagtgca ccagttttca
    21781 atgatccatg gaccacacca gctgctcctg cttcggattg catttctact acttttactg
    21841 gttgaccgaa tacgttttct tttcctgctg ctgaccattg atctactaat tcagccatgg
    21901 tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg
    21961 cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat
    22021 attctctctt attatctgtt ccattataac gtaaaacatg ccattttttt cttttattta
    22081 taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac aaacagtcta
    22141 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    22201 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    22261 atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt attgccgaca
    22321 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    22381 agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg tgatgcaccg
    22441 gtatctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa tattcgatac
    22501 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    22561 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    22621 agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg atgaatctat
    22681 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtgggac
    22741 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    22801 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
    22861 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat
    22921 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
    22981 tttcatacaa aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac
    23041 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
    23101 tttatgtatg ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa
    23161 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
    23221 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc
    23281 tcagctaata gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat
    23341 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
    23401 tggattagta gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact
    23461 aataagacgg attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc
    23521 tatttcaggt gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt
    23581 aggattaggc tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac
    23641 ataatatatt cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc
    23701 acttgctaaa tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa
    23761 tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata
    23821 ttctaatcta aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa
    23881 tattattcta cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat
    23941 gattaatatt ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc
    24001 tcacatatta gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt
    24061 cgctatgaat tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat
    24121 gatttggctt gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg
    24181 gttcagatat ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt
    24241 ctccgaaatt aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc
    24301 ttttaaaacc ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta
    24361 cattttcccc catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc
    24421 caaaaggtag atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca
    24481 tagaacctgc gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt
    24541 taatctgtcc atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg
    24601 gttcgggtat tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca
    24661 aaaaaactcc tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc
    24721 tcattgatcc attgtattcc attttattcc ccctattact attcatatcc gcctatctat
    24781 atcgatcttc caatgcagag ataattgctt ccataccttg attttttact gagttaccta
    24841 tgcgagatat cattctccac taaggtgcat catattgtct atctaacatg attaattctc
    24901 caataggtgt atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct
    24961 accatgtttg ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct
    25021 ggattaaata taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct
    25081 tttgaatgtc cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt
    25141 taccctgacc attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc
    25201 tcctaacttc actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc
    25261 atttaattcg gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt
    25321 atcaataaac tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca
    25381 taatatcatt gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat
    25441 tgacgaaaaa gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa
    25501 ctatttgtaa tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga
    25561 ttcccaagca aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc
    25621 ttatataaat tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg
    25681 cttatatcac aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt
    25741 tttcaaattg tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt
    25801 taacattatc atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta
    25861 taattaacct tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat
    25921 ctctcctaat ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat
    25981 acatactgcc tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc
    26041 gttcttactt ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca
    26101 acaataagaa tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat
    26161 aagtaggatt agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag
    26221 cacaaacacc aaagataaca actccaaaca ttgaattttt atacaatgac catttattta
    26281 taggcttttc tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag
    26341 ttcataagcc ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc
    26401 ttgttttaag cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg
    26461 ccgaaaagtc tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac
    26521 tatttgtgaa acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag
    26581 tagctcttaa ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat
    26641 cccctctaaa aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa
    26701 tagagcatta ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca
    26761 attatttgt
//
LOCUS       DAZUKT010000037        26642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000037 DAZUKT010000000
VERSION     DAZUKT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.037"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            477..719
                     /locus_tag="ACG816_001498"
     CDS             477..719
                     /locus_tag="ACG816_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFS4920380.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            716..1186
                     /locus_tag="ACG816_001499"
     CDS             716..1186
                     /locus_tag="ACG816_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4920381.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1314..1394
                     /locus_tag="ACG816_001500"
                     /pseudo
     CDS             1314..1394
                     /locus_tag="ACG816_001500"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1401..2528
                     /locus_tag="ACG816_001501"
     CDS             1401..2528
                     /locus_tag="ACG816_001501"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4920382.1"
                     /translation="MSREYYNRIEKGKQPLNDELREIIEKQIERFNPQEPLFLLIDYF
                     RVRFPTTDALAIIRDVLQLKSNYMLYEDYGKYGYESKYVLGDINIMCSMQEHLGVLLE
                     LKGKGCRQMECYLLAQERSWYDFMLDCMTAGGVMKRLDLAINDKAGILDIPKLKEKYK
                     AGECMSYFRMQKDYSGTEKCGSDLPKNTGETLYLGSTSSELYMCAYQKNYEQYVKNGT
                     EIEDTEIKNRFEIRMKNERAYYAVVDLLTYRDAERTAFSIINHYVRFVDREDDKPKSQ
                     WITNDDWAWFVGENREPIRLTTKPEPYTLQKALHWLQRQVAPTIKMVQALDRENRTTI
                     LKDMIEQAELKDKHKHLLQLEKSTIEERIDTAVPQENDGIF"
     gene            3517..3726
                     /locus_tag="ACG816_001502"
     CDS             3517..3726
                     /locus_tag="ACG816_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HFS4920383.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3838..5451
                     /locus_tag="ACG816_001503"
     CDS             3838..5451
                     /locus_tag="ACG816_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4920384.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5486..6697
                     /locus_tag="ACG816_001504"
                     /pseudo
     CDS             5486..6697
                     /locus_tag="ACG816_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6720..7646
                     /gene="glsA"
                     /locus_tag="ACG816_001505"
     CDS             6720..7646
                     /gene="glsA"
                     /locus_tag="ACG816_001505"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4920385.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7854..9314
                     /locus_tag="ACG816_001506"
     CDS             7854..9314
                     /locus_tag="ACG816_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920386.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9535..11298
                     /locus_tag="ACG816_001507"
     CDS             9535..11298
                     /locus_tag="ACG816_001507"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4920387.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11382..11840
                     /locus_tag="ACG816_001508"
     CDS             11382..11840
                     /locus_tag="ACG816_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4920388.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11949..12632
                     /locus_tag="ACG816_001509"
     CDS             11949..12632
                     /locus_tag="ACG816_001509"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4920389.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12779..13393
                     /locus_tag="ACG816_001510"
     CDS             12779..13393
                     /locus_tag="ACG816_001510"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4920390.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13409..13615
                     /locus_tag="ACG816_001511"
     CDS             13409..13615
                     /locus_tag="ACG816_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920391.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      13840..14006
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            14180..15286
                     /locus_tag="ACG816_001512"
     CDS             14180..15286
                     /locus_tag="ACG816_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4920392.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15378..16133
                     /locus_tag="ACG816_001513"
     CDS             15378..16133
                     /locus_tag="ACG816_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4920393.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            16191..18230
                     /locus_tag="ACG816_001514"
     CDS             16191..18230
                     /locus_tag="ACG816_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4920394.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVLQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18339..19490)
                     /locus_tag="ACG816_001515"
     CDS             complement(18339..19490)
                     /locus_tag="ACG816_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4920395.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWIKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19644..20174)
                     /locus_tag="ACG816_001516"
     CDS             complement(19644..20174)
                     /locus_tag="ACG816_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4920396.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20273..20623)
                     /locus_tag="ACG816_001517"
     CDS             complement(20273..20623)
                     /locus_tag="ACG816_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4920397.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20780..22309)
                     /gene="ahpF"
                     /locus_tag="ACG816_001518"
     CDS             complement(20780..22309)
                     /gene="ahpF"
                     /locus_tag="ACG816_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4920398.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22319..22882)
                     /gene="ahpC"
                     /locus_tag="ACG816_001519"
     CDS             complement(22319..22882)
                     /gene="ahpC"
                     /locus_tag="ACG816_001519"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4920399.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(23026..23688)
                     /locus_tag="ACG816_001520"
     CDS             complement(23026..23688)
                     /locus_tag="ACG816_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4920400.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23872..25518
                     /locus_tag="ACG816_001521"
     CDS             23872..25518
                     /locus_tag="ACG816_001521"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4920401.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25509..26591
                     /gene="uxuA"
                     /locus_tag="ACG816_001522"
     CDS             25509..26591
                     /gene="uxuA"
                     /locus_tag="ACG816_001522"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4920402.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         9045 a         4558 c         5331 g         7708 t
ORIGIN      
        1 atttttccgc catgcttgca agggggcggt tcgcttcggc ggttcggcga gcttgcgagc
       61 cgttcaaagc ccccgttatc taacaagggg ggcacaaaaa acaagaaaca ctatacacta
      121 acaagcggaa aacgctgtat ttataaggca aaaccccatt tttacagtag tgatataaat
      181 tgtacctata tcactatgaa aattgaatag taaatatgag aaagacagtt gatttcaaaa
      241 aataagaaat tgattgtcgt tttcttttgc agaaatttaa gtgcaggcaa ttatggtagt
      301 tgtcgtaatt gctgtcagct cagtgtttat cactgatggt gtgaaagatt tacggttgga
      361 gatgttcaat aagattattg gtgcttaatt atttttattg tagtgctgat atgagaatgt
      421 tataattggg gtataaaaca tacaaataaa tttgctgaaa aatgaggtat caaaatatgg
      481 aaaatgaatt actggaaaac ttaggaagac ttcacacaac tgaacttggc gttattagaa
      541 ttaaaaagaa cttatctttg aatgtagaaa atgtaattga atggtgcaaa gaaaaaatta
      601 gcctttctaa tgcggaaatc acaagaaaag gaaaaaactg gtatataacg attgataatt
      661 gtattataac ggtaaatgcg tacagttata ccgttatcac tgctcacaag gtaaaatgat
      721 aaatttttct ataaaggaat taaacgcatt tgcagttatt gggcaggaag tagaactgac
      781 taattatcaa aaaaagaata ttcagattag tacacagttt tggagaaagt ttaacagtag
      841 tttgaaaaaa gcgtatcttt cacaatcagg aaattgggta aaatatgctt ttatggaaag
      901 aagaaatgga aaactttatt atttctgttc tattccccaa agaaccatta ccccagaggg
      961 ctttctgtat aaagaaatac cgtcttataa atatttggtt gtggagcata ttggtgctat
     1021 ggataaaata tatgaaactt atggcaagat ttatgaagaa attattccta gtacatcgta
     1081 tattcctata aaagatatta tcctacactt tgaaaaatac gattatcgtt ttcattggaa
     1141 tagtgataat tcagttattg aaatttggat acctattcaa gattagaagc aattttatat
     1201 ccatacacac aaagcagtta gtcttaggat tgactgcttt ttctatacag atttttaatg
     1261 acaggcaatt atcaattatg gtagttgcct tttttgattg gaggaattga agaatgaatg
     1321 ataaaaactt tattgaagaa ctaagacaaa agcgtgagga atacggagta acacaaacaa
     1381 ggcttgctgt tgcctgtggt atcagccgtg aatattacaa ccgcattgaa aaaggaaaac
     1441 agccattgaa tgacgaatta agagaaatca tagaaaaaca gattgagcgt ttcaatccgc
     1501 aagaaccact attcttattg attgattact ttcgtgtccg ctttcctact acggacgcat
     1561 tggcgattat ccgtgatgta ttacaactga aatctaatta catgctttat gaagattatg
     1621 gaaaatacgg atatgaaagc aaatatgtac ttggcgacat caatatcatg tgttccatgc
     1681 aggagcattt aggtgtttta ttggaactga aaggcaaagg ctgtcggcaa atggaatgtt
     1741 atctgctggc acaggaacgc tcatggtatg acttcatgct ggattgtatg acggctggtg
     1801 gtgtgatgaa acggcttgat ttggctatca atgataaagc aggaatattg gatattccaa
     1861 agttaaagga aaaatacaag gctggcgaat gtatgtccta cttccgtatg cagaaagatt
     1921 acagcggtac agaaaaatgc ggtagcgatt taccaaagaa tacaggagaa accttatatc
     1981 tcggttcaac aagtagcgaa ttatatatgt gtgcttatca gaaaaattat gagcagtatg
     2041 tcaagaatgg cacagaaatt gaagatacgg agattaagaa ccgttttgaa atacgcatga
     2101 agaacgaacg agcctattat gcggttgtag atttactgac ctatcgggac gctgaacgca
     2161 ccgctttttc tatcatcaat cattatgtcc gctttgttga cagagaggac gacaagccaa
     2221 aaagtcaatg gataacaaat gatgattggg catggtttgt aggggaaaac agagagccga
     2281 tacgcttaac cacaaagcca gagccttaca ctttacaaaa agcgttgcat tggctacaaa
     2341 gacaagtcgc accaaccata aaaatggtac aggcattaga cagagaaaat cgtacaacga
     2401 tactgaaaga tatgattgag caggcagaac taaaagacaa gcataaacat ttattacaat
     2461 tagagaaatc aaccatagaa gaacgtatag ataccgctgt tccacaagag aatgacggta
     2521 ttttttaatg taattgaaaa atttcagatt tagtcagcaa agagctgttt tcattcccta
     2581 tgtgaagtga aagaagaaaa ataaattttt tattaaatct gcaacaaaat ggctacttgc
     2641 gtacagatat ggtgcgagaa aaggaaatct aaactttttt tgaaaatagg tcattaaatc
     2701 ggggcttgct gtccctatat gaagtgaaag aagaaaaaag ttttgaaaaa atgacggaaa
     2761 ttcgtgatta gtggacagtc ttctgtgaaa agaggaaaaa catcatcatt ttgtattcaa
     2821 acaggcatta gcagttgagt gttgatgtga aaagatgaaa atactttgtt ctcaacttaa
     2881 caatttaagg tttccgttcc ctatatggtg caaagaaaat atttcattct attgttggca
     2941 gtaacggtat ggtttgggta gttagggtgt gaaaaggaaa atattctttt ttcatcatca
     3001 acttagcaaa atgacatttt ctatccctat atggtgcatg aaaagaagtt aaaatttttc
     3061 catttaaact tagcaaatgg acgtttgctg tacagatagt agtgaaaaag gagaaaatca
     3121 accgttgatt gccaaaatga accgttccat ttgtactaat aatggaaaaa gttttggatt
     3181 ttggttacgt ttctcctctt ttccaacgcg atatatagga aagacgattt ttttctttca
     3241 atcgttcttt gacaactgaa taaagctatt caatacgttt gacagacagc tagccgttga
     3301 aacaaatacg ccacgacctt tcccctgcaa gagcgagcga aaacctattt gtgttcttgt
     3361 aaaagatttg tcctctttta tcgccatgac ctgtactgtc agataatgat actcccgtac
     3421 agccacagct tgagcattaa gagcgtcgca cgcaatgggt acggctacat aagaactatg
     3481 cagaggtgga actcctgtgg gctatgacaa aagccgttga attgcttaga aagattttac
     3541 agaaaggggg tatgtgttat tgataatgct gtaaaaacaa ttcaaaagaa aaatggcaag
     3601 tgtggcattg gcaacagagg aaagacaaag attgtagtaa aagagcattt ttccgaaaag
     3661 ggcaaaacaa tggaagaact tctaactgat gtcatgctgg aaaaagcaaa gcagacaatc
     3721 gcataaaatc ggtgcagttg taagaaatag attgaatgac aaaaagtacc cgtgttatac
     3781 tttacttgca agcaggtatt gtaaacacgg gttagttata aaggaggata actgacaatg
     3841 aaacaacaga tttacaatac tgcactttat cttaggttaa gccgagatga tgaattacag
     3901 ggcgaaagtt ccagcattac cacacagaga agtatgttgc gtttatatgc aaaagaacat
     3961 catttgaatg tcattgatga atatattgat gacggctggt cgggaacaaa ttttgacaga
     4021 ccgagttttc aaagaatgat tgaggatata gaggcaggaa aaatcaactg tgttgtaacg
     4081 aaagaccttt cccgtcttgg cagaaattat attatgacag gacaatatac agaattgtat
     4141 ttccctagtc ataatgtccg ctacatagcg attgatgacg gtgtagacag cgaaaaaggc
     4201 gaaagtgaga ttgcaccatt taagaacatc atcaatgaat gggtggcaag agatacaagc
     4261 cgtaaagtca aatcagcatt taagacaaag tttgcggagg gggctcatta cggtgcttat
     4321 gctccgttag gatataagaa acaccctgac atcaaaggaa aactgttggt tgatgatgaa
     4381 acaaaatgga ttattgaaaa aatcttttcc ctagcctatc aaggttacgg aagtgcaaaa
     4441 atcacaaagc agttaagagc agaaaaagtt ccgacagcgt catggctaaa ttttacaaga
     4501 tacggtactt ttgcacatat ctttgaggga aaacccgaaa gtaagcgtta tgagtggacg
     4561 attgctcatg tcaaggcgat attgaaaagt gaggtttata tcggaaacag tgttcacaat
     4621 atgcagtcta cggtatcgtt caagagcaaa aagaaagtgc gtaagcccga aagtgaatgg
     4681 tttcgagtag aaaacactca cgagccgatt attgacaagg aagtgttcta tcgtgtgcag
     4741 gagcagataa aatcaagacg cagacagaca aaggaaaagg caacgccgat ttttgcaggg
     4801 cttgtcaagt gtgcggattg tggttggtct atgagatttg gaacgaataa gacaaataaa
     4861 acgccttaca gttattatgc ttgcagttac tacgggcagt ttggcaaagg taattgttct
     4921 atgcactaca ttcgttatga tgtgctgtat caagccgtat tggaacgctt gcagtattgg
     4981 gctaaggcag tacagcagga cgaagaaaag gtattgaaca agatacagaa agctggcaat
     5041 gcagagagaa tacgggaaaa gaagaaaaag gcaagtacat tgaagaaagc cgagaacaga
     5101 caaaatgaga ttgaccgttt atttgcgaaa atgtatgaag atagagcctg tgagaagata
     5161 acagagcgaa attttgtgat gttgtccagc aagtaccaaa aagaacagat agaactggaa
     5221 cagcagataa caagcctaag agaagaacta agtaaaatgg aacaggatat gataggtgct
     5281 gaaaagtgga ttgagttaat caaggaatat tccgtaccaa aggaactgac agcaccatta
     5341 ttaaatgcca tgatagaaaa aatcctcata catgaagcaa caacgaatga ggataacgaa
     5401 agaatacagg aaattgagat atattaccga tttattggaa gagttgagta atcaacaaga
     5461 gtaatatttt taactaaggg aaaccggcgg tgtctatact tgggtcagtc gcacattggg
     5521 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     5581 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     5641 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     5701 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     5761 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     5821 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     5881 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     5941 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     6001 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     6061 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     6121 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     6181 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     6241 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     6301 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     6361 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     6421 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     6481 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     6541 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     6601 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     6661 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     6721 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     6781 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     6841 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     6901 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     6961 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     7021 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     7081 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     7141 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     7201 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     7261 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     7321 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     7381 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     7441 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     7501 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     7561 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     7621 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     7681 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     7741 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     7801 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     7861 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     7921 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     7981 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     8041 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     8101 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     8161 cgagtacaga actggctgat cttgaattta tttctttgag taaagtatat acaatcaaaa
     8221 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     8281 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     8341 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     8401 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgagaaatag
     8461 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     8521 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     8581 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     8641 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     8701 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     8761 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     8821 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     8881 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     8941 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     9001 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     9061 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     9121 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     9181 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     9241 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     9301 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     9361 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     9421 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     9481 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     9541 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     9601 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     9661 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     9721 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     9781 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     9841 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     9901 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     9961 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
    10021 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
    10081 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
    10141 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
    10201 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
    10261 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
    10321 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
    10381 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
    10441 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
    10501 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
    10561 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
    10621 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
    10681 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
    10741 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
    10801 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
    10861 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
    10921 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
    10981 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
    11041 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
    11101 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
    11161 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
    11221 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
    11281 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
    11341 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
    11401 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
    11461 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
    11521 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
    11581 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
    11641 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
    11701 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
    11761 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    11821 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    11881 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    11941 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    12001 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    12061 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    12121 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    12181 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    12241 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    12301 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    12361 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    12421 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    12481 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    12541 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    12601 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    12661 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    12721 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    12781 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    12841 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    12901 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    12961 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    13021 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    13081 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    13141 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    13201 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    13261 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    13321 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    13381 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    13441 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    13501 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    13561 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    13621 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    13681 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    13741 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    13801 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    13861 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    13921 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    13981 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    14041 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    14101 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggattttggg
    14161 tccttatttg actttgtcca tgatcggtct tttagaagta tacagtgcca gttcttatcg
    14221 tttattgcaa gccgatgaaa atacaaaaag tctattattg cgtcaactaa ttttcatatt
    14281 tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa ctacactatt tacttcaccc
    14341 taaaatagca ggatacggtt tagccttatc gattttcttt ttagtattag taagaatagg
    14401 gatattcggt gtcactgtca acggcgcaca acgttggatc tctctgtttg gcattcaatt
    14461 ccagccttct gaactggcaa atcttttttt gattttttat ttaagctggt tttttcgtga
    14521 cggaaatagt agcccaaaag atctaaaaaa accattcctg attacagtag gtataacttt
    14581 tctgatttta tttcagccaa agattgctgg agcattgatg atcctttcga ttgcgtgggt
    14641 catattttgg gcagcggcgg ttccatttaa aaaagggatc tatctaatcg ttactttttc
    14701 tgcattgctg attggagcag caggcggggt attatattta ggaaataaag gttggcttcc
    14761 acagatgttt aatcatgctt atgaaaggat cgcgacttta agagattctt ttatcgatag
    14821 tcatggtgcg ggctatcaaa tgacccattc gttttatgca ctatataatg gtggcatatt
    14881 tgggcgggga ttaggaaata gtatcacaaa aaaagggtat cttccagaga cagaaacaga
    14941 ttttattttt tcgattatta cagaagagct tgggctaatc ggtgcattgt gtgtgctttt
    15001 cttattgttc tcattatgca tgagaatctt ttgtttaagc agtcgctgta aaaatcagca
    15061 agctggattg tttcttttag gattcggaac tttgctattc gttcagacga taatgaacgt
    15121 gggaagtatc gcggggctga tgccaatgac tggtgtgcca ttgccatttg ttagctacgg
    15181 aggaacaagt tatctgatcc tttctttagg aatcggaatc acgttgaata tttcttcaaa
    15241 gatacaggcc gaagagttgg cgctctatcg acctgaaaaa caataaagag aaactgagaa
    15301 actctcagtt tgaattatca taaagtacaa tcttttattt ttcaagcgag aggttctttt
    15361 agaaagagga attcgcaatg aaagtatctc aaaaaatcat attatctatt ttgttactgc
    15421 ttacattagt agttggctat ttttcgattg agtttatcca tggattttcg tctgcaaaac
    15481 agagttcaac tgtgaaaaag gtagagccga aaagtatccc taccacacta aatgtggctt
    15541 tgattggttc agatgctcgt tcgaaagacg aaaacggtcg ctcggattca cttctggttg
    15601 cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg
    15661 tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg
    15721 atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta
    15781 tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg
    15841 atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg
    15901 atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga
    15961 ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt
    16021 caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc
    16081 cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac
    16141 aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa
    16201 gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag
    16261 cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag
    16321 ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag
    16381 ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct
    16441 tagataaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc
    16501 taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca aagatgaata
    16561 cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc
    16621 aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag
    16681 taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat
    16741 tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag
    16801 gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta
    16861 tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca
    16921 ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat
    16981 attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag
    17041 aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg
    17101 agatggcttt tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg
    17161 cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca
    17221 aattgacaat cgatgcgaag gcacaaaaaa cagcttttga cagtctagga ggaaaagctg
    17281 gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa
    17341 gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag
    17401 aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt
    17461 ttaaaatgat tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt
    17521 tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatcttat caagtaacgc
    17581 gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata
    17641 tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata
    17701 aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta
    17761 atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac
    17821 ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac
    17881 ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg
    17941 aaacagcagt acaaacgatc gtgccagatc tgagagaagt tctgcaagat gtaaatggta
    18001 cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa
    18061 tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg
    18121 ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa
    18181 ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt
    18241 tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt
    18301 tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata aacttttttt
    18361 gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa
    18421 ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa
    18481 gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa
    18541 ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc
    18601 aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca
    18661 ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact
    18721 tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag
    18781 ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg
    18841 ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat
    18901 ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa
    18961 gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg
    19021 actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact
    19081 acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc
    19141 ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa
    19201 acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc
    19261 aaaagagata aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc
    19321 gaaaagtcat ggactagaat atcaggtttg atccaattca atccagcttg gcctagcaaa
    19381 acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag
    19441 ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt
    19501 cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa
    19561 tatactcaca aaaaaaccaa caatccaaaa gatttttttg gattgttggt ttttatacgg
    19621 tctattttaa taactaaaaa tgattaacgt atagtcattt ttcttattct gatgaaattg
    19681 ataatctcct ggatgcccat gaaaatcaaa ataaagccga atatttggaa tagaagcatc
    19741 aaacttttga atggattgaa caataagacg aatccgccaa tcaacaacag aatactacta
    19801 taaacactcc aacttttagc ttttgtattc atgctgaaga acaactggaa caagctgttg
    19861 atgataacag ctaatccaag aagaatcggc aggatagaaa cgattgcagg tgcaaaagca
    19921 tagacgatca gagctaagat caggaaaaaa agaccactga acagaccgat actccaagaa
    19981 cctgttttcc ttttaaaacg gtaagcctca aaaagattca acaacccaag caaaacaaga
    20041 taggcggtga tcagataacc gataaaatga aaaacagcag aagggtttag cacaatcgca
    20101 atcccagaaa taatataaac agcagctctt aataaggcgt atttttgaat attttgttta
    20161 atcgtctcaa acatataaga aaacctcctt tgacttattt taacgtgaac tagtcatttt
    20221 accaaattaa atgttacaaa atgagcgaag gagaagaata tctctggaaa aatcaacttc
    20281 tgaggatctt ttccattttt ttgccttttg ctagctcatc gatcatttta tctaaataac
    20341 gaatttcttt catcaagtta tcctcgatgt cttctactcg tacaccacaa acaacacctg
    20401 taatcaagga acgtgaagga ttggggacag gggcttccgc aaaaaattgt tcgaaagtca
    20461 cttgtttttg tagttgatat tctagttctg tttgggaata acccgtcagc caacaaatga
    20521 tttcatctac ttcctctttt atacgatctt ttctctccgc ttttgctaca tacataggat
    20581 aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc cataatcagt cacctttctt
    20641 tttttcaaat tatttactac ttatagtata taagaaaaag agaagaaaaa gcgattattc
    20701 aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat ccttttttcc gctctctgac
    20761 tttttacaat cttcatagct caagtgcgca tcagataatc gaatgctcct aaagctgcag
    20821 tagctcctga acccattgaa acaatgattt gtttgtatgg actatctgta caatcaccag
    20881 ctgcgaaaac gccggggata ttcgttgcgc catttttctc ggtcactatt tcatttcttg
    20941 gattcatttc gatcgttcca tctagccaat ccgtattagg gagtaagccg actagaatga
    21001 atactcctga aacagctagt gtatgcatct ccatcgtacg gcgatcgata taggagagag
    21061 attctacctg attcgtaccg ctgatttctt tcgtcgctgc atgtttgata acagtgacat
    21121 tcgcaagaga gactagttta tcctgtaaga tttgatctgc ttttaattct ggtaagaatt
    21181 ctagaacata tacatgtttt gctaacccag ccaaatcgat tgctgcttca acaccagaat
    21241 taccaccacc gataacagca atctcttttc ctttaaataa agggccgtca cagtgtgggc
    21301 aataagcaat tcctttattt ttaaactctt tttcgcctgg tacattgata gaacgccagt
    21361 gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga tataccgtta gctaagccaa
    21421 tattaataaa atgttgctct ttatttaagg aaacggcctg ttgattcatc ataatgtcga
    21481 tagggtaact acgcacatga ctttctactt gctgcatcaa ttttggacct tctgtgtaag
    21541 aggtaccaat catattttca atccctagtg tttcaacgac ttgtccgccg aaagaatcag
    21601 cgacaagtcc tgtacggatt cctttacgtg ctgcatagat agctgcacta ctagctgctg
    21661 gtccaccgcc aatcactaat acatcataag gttctttttc aaacaactct tcttcaatca
    21721 aagggccagt gattttttca acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt
    21781 ctccatttaa aaacacagtt ggcactgcca tgattttttt atcatcgatt tctttttgga
    21841 acatcccacc ttcaatcatc gtatgagtaa tatttggatt taatacagct aatatattca
    21901 atgcctgtac aacgtcgggg caattatgac aagtcaagct aacataactg tcaaaatgca
    21961 atgtttgtgt tatttttttg attttaagac tcaaagattc actgattttc ggtgcccgtc
    22021 cactaatttg taacatagcc agtaaaaaag aggaaaattc atgaccgagg gggataccag
    22081 caaaaacgat tccactttct ttgccaactg gattgataga aaaactaggt gtgcgcatcg
    22141 aatctctttt ttcccaagag attttaggag acatttctgc tatttccttc gtaaattcag
    22201 ctagtttttg actattttca ctattatcca aactaacaga aaaaacaata ggagattcaa
    22261 tcagtgctaa atattgttcg agattttgtt tagtttcttt gtctaacata tccaccacct
    22321 aaatctttcc aacaagatca aaacttggtt tcaatgtttc gccactttct ttccattttg
    22381 ccggacaaac ttcacctggg tgggtacgta catattgagc agcgcgaatc ttatcaatca
    22441 gtgcactagc gtcacgaccg attccatctg cattgatttc catagcttga acgattccgt
    22501 ctggatcaat aataaatgtt cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa
    22561 aaatacgaga aatctgatga gacggatcac cgatcatcac gtattcgatt ttaccgatag
    22621 catctgatgt atcatgccat gctttgtgag taaagtgggt atcggtagag caagaaaaga
    22681 cttcaacacc taaagatttc aatgtactgt attgttcttg taagtcttct aattcagtag
    22741 gacaaacaaa agtaaaatca gctggataga agcaaagaat actccagttc cccataagat
    22801 ctttttcact gacttctaaa aattctcctt gatgataagc atgtgcatta aattcttcga
    22861 tttttttccc aatcaatgac atagtaaaac ctccaataat aattatttat ttataattat
    22921 tgtaatttag ttggagttgc aaagtcaatg gatattttgt tatttaataa agattactca
    22981 aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga aaatgctact gctggaaata
    23041 ctcgggataa cgttgagtaa cagtttcctg ttctttcagc atcatgtgta agtgcaatcc
    23101 cgtttgaaat cgagcttctt ctggtttttt atcaactaaa gccttgagta aatcataatg
    23161 ttgttccatg atcgtttccc atggaagccc ctgaatggtc actcgtaacc agcggaaacg
    23221 ttctaaatgg gtattatggt catctaacca gttccaaact tcttttcttc ctgcaatttc
    23281 gaaacaagct tcatgaaata aattatctgc acggaaaaaa gcgcgttcgt catttatttt
    23341 gactgctttt tcttgttctt ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa
    23401 tttggaacaa cattcaatca tgatttgctc ttccaactgt tctcggacaa agcgagcatt
    23461 ttctgcagaa acaagattaa tttttgaaac atacgtacca ctttgaggta tcgtatacac
    23521 gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag
    23581 atgttcctcc agtgcctttt cagatatttt ccttccaggt tctaagtcag catagataat
    23641 ttgtttacga atcgcttgat aagcttgaga ttgcaagctg cttttcatct ttattcctcc
    23701 aaaatcattt ctatgaacgt agtataacag aagaggtctg aaaaacaatc tggtatgtca
    23761 cattaactag taaatttgta taaatagtaa aattttggtt gacatagcga tttttattgc
    23821 ataaaattca cttgaataca aaaactaata tgttagttta ggaggggaaa aatgattgat
    23881 ttaaacaact ggaggaactt tatccctgaa ttatctcaaa aagaagtaga aatacctcat
    23941 tttgaccgtg catcattaag agaaaatgga caaaaaaatc ctgtctggat ccatttcggt
    24001 ggcggaaatc tttatcgagg atttcatgca gaaatcgctc aacgtttagc ggatctaggc
    24061 gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg aagaaataat tgaaagggct
    24121 taccaagatt ttgatgattt gattcttgaa gtggtcatgc caagcagtgg agagttcgtc
    24181 aaacgtatat tagctgcgac agctgacagt ctgtattgtc atccagattg ttctgaacac
    24241 tttgataaga tgattcgaca ctttgaaaat cctagtttac aattagttac ttttgctatt
    24301 actgaaaaag gttatcgatt gaaagataca aataataaat atataggacc gattgcagtc
    24361 gatataaaaa atggtccaaa gaagccatca catacaatga gtattcttgc tgcgctactt
    24421 tataaacgtt ttttatctgg agcttggccg attgcacttg tctctacaga taatttttca
    24481 caaaacggta gtcaattacg tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa
    24541 ggttatgtac aaaatacatt tatccaatac ttatcgaatg aagaaattgt tgcgtttcca
    24601 tggacgatga ttgatcgtat caccccgaat ccttccttgt cagttagcaa aatgttggca
    24661 aaagagggct tttcaaatat gtcgattttc caaaccgcaa aaggaaataa tattgctcca
    24721 tttgtcaata cagaggacat ccattattta atcatggaag atcattttcc caatggacgt
    24781 ccgaaattcg aaaaagctgg agtattcttg actgatcgtg caacggtaga taaagcagat
    24841 acgatgaagg tgacgacttg ccttaatcct ttgcatacgt ctttagctat tatgggttgt
    24901 ttactggggt ataggagcat agctgaggag atgaaagatc cagatttatc gctgctagtt
    24961 tctgaaatcg ggtacaatga aggcctccca gttgtggagt ctccaggtat cattgatcca
    25021 aagatctttc tggaagaagt aattactgtg cgcttttcta atccaaatat tcctgatatg
    25081 cctcagcgaa tcgccacgga tacctctcaa aaggtggcga ttcgttatgg tgagacgatt
    25141 aaaaaatata tggaaataga agttaaagac ccgcagacac ttgtattcat accattgact
    25201 ctcgctgctt ggcttcgata ccttattggc attgatgatc aaggaaaacc attcatcccg
    25261 agtccagacc cactattgac agactttcag cataagcttt ccgatattca actgggtacg
    25321 caagatacat cacatattca tgagaaaatg gctcctattt tgtcaaataa agaaattttt
    25381 ggtgtagatc tttaccaagc agagcttggc agaaaaatag aagaaatgtt catcaagatg
    25441 gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt acttacaaga acatggagga
    25501 aatttatcat ggaaatgact tttagatggt acggatcaca ggaagaaaag attaccttag
    25561 atgaaatcag acagattcct gcggtcaaag ggattgtagg cacattgatg gatataccag
    25621 taggagaggt atggcctagg gagaggatca aagaactaaa agaagaaatt gagtctcgtg
    25681 ggttaacact aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa attggattac
    25741 cttcccgtga gcagtatatc gaaaactata agcagacaat ccgaaacttg gcagaatatg
    25801 gaatagaagt catctgctat aattttatgc ctgtatttga ctgggtgaaa tctgaccttg
    25861 actaccgctt agaagacggc tcatccacgt tagcttttat atctgatgat attccagatg
    25921 atccaagaga aatcgtcgcg cgcatcgaac attccagcaa tgaatttgaa ttgccgggat
    25981 gggaacctga gcgcttagct catatcaaat ccttgcttga agcctatgca tcagtagacg
    26041 aagaaaaact tcgagaaaat tttgcctatt ttctccaatc aatcatccca acatgtgaag
    26101 aggtaggggt caaaatggct gtacatccag atgatccgcc atactcgatt tttggattac
    26161 ctcgaatcgt aaagaatcga gaagatttag attggttgtg ccgcatagta gacagccctt
    26221 ataacggaat cactctttgt acagggagta ttgcagaaga tccagaaaat gatgtatatg
    26281 ggattttagc agaattttgt caaagagatc gaatcccatt tgcacatgtt cgaaatatta
    26341 aatttttagc taatcgagat ttctatgagg caccgcataa atccgaatac ggttcattag
    26401 acatgtaccg catactaaaa acaatgtatg acaacggatt taacggatac atccgtcctg
    26461 atcatggaag aatgatctgg aatgagacag gaagaccagg atacggctta tatgatcgag
    26521 cattaggtgc tgtttatcta aatggattat gggaagcaat cgaaaaagga aacaaaagta
    26581 aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat gttcaaattc
    26641 tt
//
LOCUS       DAZUKT010000038        26632 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000038 DAZUKT010000000
VERSION     DAZUKT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.038"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1734)
                     /locus_tag="ACG816_001523"
     CDS             complement(34..1734)
                     /locus_tag="ACG816_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920403.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2241..3401
                     /locus_tag="ACG816_001524"
     CDS             2241..3401
                     /locus_tag="ACG816_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4920404.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3417..3860
                     /locus_tag="ACG816_001525"
     CDS             3417..3860
                     /locus_tag="ACG816_001525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4920405.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4343..4873)
                     /locus_tag="ACG816_001526"
     CDS             complement(4343..4873)
                     /locus_tag="ACG816_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4920406.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4978..5529
                     /locus_tag="ACG816_001527"
     CDS             4978..5529
                     /locus_tag="ACG816_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4920407.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5682..6155)
                     /locus_tag="ACG816_001528"
     CDS             complement(5682..6155)
                     /locus_tag="ACG816_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4920408.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6293..7765
                     /locus_tag="ACG816_001529"
     CDS             6293..7765
                     /locus_tag="ACG816_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4920409.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7762..8472
                     /locus_tag="ACG816_001530"
     CDS             7762..8472
                     /locus_tag="ACG816_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4920410.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8824..9108
                     /locus_tag="ACG816_001531"
     CDS             8824..9108
                     /locus_tag="ACG816_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4920411.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9425..9808)
                     /locus_tag="ACG816_001532"
     CDS             complement(9425..9808)
                     /locus_tag="ACG816_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4920412.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9810..10394)
                     /locus_tag="ACG816_001533"
     CDS             complement(9810..10394)
                     /locus_tag="ACG816_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4920413.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10441..11916)
                     /locus_tag="ACG816_001534"
     CDS             complement(10441..11916)
                     /locus_tag="ACG816_001534"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4920414.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12097..12408
                     /locus_tag="ACG816_001535"
     CDS             12097..12408
                     /locus_tag="ACG816_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920415.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12523..14859)
                     /locus_tag="ACG816_001536"
     CDS             complement(12523..14859)
                     /locus_tag="ACG816_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4920416.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            15001..15669
                     /locus_tag="ACG816_001537"
     CDS             15001..15669
                     /locus_tag="ACG816_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4920417.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15672..16715
                     /locus_tag="ACG816_001538"
     CDS             15672..16715
                     /locus_tag="ACG816_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4920418.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16952..17833)
                     /locus_tag="ACG816_001539"
     CDS             complement(16952..17833)
                     /locus_tag="ACG816_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4920419.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17992..18720
                     /locus_tag="ACG816_001540"
     CDS             17992..18720
                     /locus_tag="ACG816_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4920420.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18876..19193
                     /locus_tag="ACG816_001541"
     CDS             18876..19193
                     /locus_tag="ACG816_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4920421.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19186..19794
                     /locus_tag="ACG816_001542"
     CDS             19186..19794
                     /locus_tag="ACG816_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4920422.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19849..20640
                     /locus_tag="ACG816_001543"
     CDS             19849..20640
                     /locus_tag="ACG816_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4920423.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20778..21266
                     /locus_tag="ACG816_001544"
     CDS             20778..21266
                     /locus_tag="ACG816_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4920424.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21317..22189
                     /locus_tag="ACG816_001545"
     CDS             21317..22189
                     /locus_tag="ACG816_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4920425.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22365..22727
                     /locus_tag="ACG816_001546"
     CDS             22365..22727
                     /locus_tag="ACG816_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4920426.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22736..23488
                     /locus_tag="ACG816_001547"
     CDS             22736..23488
                     /locus_tag="ACG816_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920427.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23502..25304
                     /locus_tag="ACG816_001548"
     CDS             23502..25304
                     /locus_tag="ACG816_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920428.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25581..26621
                     /locus_tag="ACG816_001549"
     CDS             25581..26621
                     /locus_tag="ACG816_001549"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4920429.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         8487 a         4638 c         5392 g         8115 t
ORIGIN      
        1 agttttttga tcggatgtat ttatcccttt tgcctaagca cgtaaaagct tgtatctatg
       61 ataaataaat aaacatagca aggtaaacca gccaatcaaa gtaagagaaa gaatgaaata
      121 gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg attccttttt gctgcttcac
      181 gattggggtc ccgatatctg ttacttcata atcagtcaat gtttttccat tttgctgtag
      241 cacagtccga tcgtagacga taacagggat atgaacagtt ttgtccccag tgtttttcca
      301 cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa gttgtttcgt tttctagtat
      361 ttcttcgtta taccggtcgt atttattgtc ttttgtctcc ttatagattg gcagatagtc
      421 aggtgtggtt ttttggaaaa cgaacaagct ggtagacata tctttgctga agaatgtttt
      481 ccttgcttct tccggcgtat cgaatagata agtatgtcga ctgacaaatg tttcattttc
      541 ccacttttcc agatgctggt ataagttctg gatcgtctgt gctgaaaaaa taagaatgcc
      601 aaatatactt aaaagacgga accacttttg ttcagaccac tggttcagtg aaagggcaag
      661 gtaaagtaga agcaatagcg taaatggcac gaaaaatcga aatggaaact ggatcaaact
      721 aataaaaggc acattttttt cgatcaatgt cgtccatgga atcaagctag tagaaagaaa
      781 aaagaaaaca agacttgtgt acaacacgat tttttggaat aaggtgttct tttgttgaaa
      841 gaaaataaaa agcaaaaaac ctgataatat caatatagga aaggccttag ggtaatggag
      901 ccaataactg ccatccagat ctatcgcctt ttgactcatt tctctgttaa caaagggagg
      961 aagaatctta ttcccactgt aaacaatagc taatcctgcc caaatatttg ctgtcagaac
     1021 aaaataaagc aagaccgact taatcagttg aagcagtgct tttccttttt cagtctggtt
     1081 aaaaaagtaa taaagataaa aagggatata catcaaagct aacatgatcg aagacagtaa
     1141 atggatctgt gtcataagtc ccactgagac agcgactttg ataacaggga actttttatg
     1201 aaggacgaaa tcagcaatag ggatcataca taaaggataa aaacaagtcc cccagctagt
     1261 gaatccttgg ttgatagacc agtattgaat agaatacgta ctcatgaata aaacggctac
     1321 tggtacactt acccaatttc gaacattgac atatctcagt aaaatcagca tagaaaatcc
     1381 tgaaataaga taaagaatga aatttgaaag aatctgataa tgaaaccagc tacctgcaag
     1441 caacacgatc aatccttgta aataagcgaa aaatgggccg taaacggcat tcacgatcct
     1501 gccactttgc tggaatccat aaagggaagg aaaataatgg aaatccccat atttaatttg
     1561 ttgcgcggca tcatagaacc gattgtaatg gaatggggca tcagcaccca aaattagttt
     1621 atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg aaaaaaattg ctaaaaatgg
     1681 gccatatttt ttcaattgtc gaactccttt gcaaacttta gataaattca tcatatatcc
     1741 tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa tatttatgaa ttttacaaga
     1801 aaaacataag tgaaagttct aaggagagag gatgcgacat aagttttgtt gcatctcctt
     1861 gttttgaaac agaatttttc ttaatactcg aaacaaagta gctttttcat gacctctcgg
     1921 taaacggtgt tcaatcagct gcttctcggt aataagctca ctagcctaga aaatatgaga
     1981 agctattttc tgactagcgc tcttattact caaagcataa cgcagttttc acaacctctt
     2041 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaacccaa caaaaaatat
     2101 gagtagctat ttttttgggc tgtacttatt acttgatgca ggacagcttt ttcacaacct
     2161 catcggcttt cattttctat ttttttcctg aacccatata ataaaagaaa gaacaaaaaa
     2221 acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa acagtctcgt cgacttttgt
     2281 ttcgtttgtt tttattaata tgtatcgtcg tagggttact gatcgcttta tatccatttt
     2341 atgtggattc tttaaatagt tttattgatc aaaaacgtat cgaacaagta caaaaacgga
     2401 ccgcagctga aattgaagtg cagcgaaaga aaatggagga gaaaaaccaa cgattaactg
     2461 accaaggatt caatcctggt actgatcctt tcaatgaaaa gaacagaaat gaaagtacaa
     2521 catccagcca attagaggaa tggctgatcg gaagtatcaa tatacctaag atccaattaa
     2581 atatttcact ttatgatcgg ctcaatggta ggatccttga aaatggagca ggcgtcttgc
     2641 aaggaacctc tttcccgctt ggtggaaatt cgacgcattc agtgatctct gctcacagcg
     2701 gtttacctaa tcgacggtta tttaccgaat tagatcgttt agaaaacgga gacacgttta
     2761 ttttgactgt tttgggggag aaattggctt atcaagttaa aaatatacaa gtggtactgc
     2821 cagatgacac gtctgttcta acgatttcag aaggagaaga cttagttacc ttgcttacat
     2881 gtacgccgta tatgatcaac acgcatcgtt tgctagtgac aggacatcgg attccttata
     2941 acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga aagaaccatg cggcaatggt
     3001 ttattcttgc aggaacgata atagctgtag tcatactgtt agtgtttatc ggacgactaa
     3061 tctatcagta tagattaagt aaaaaagtgc ttgatttttc atttattatt agtgattcgt
     3121 cagggaaacc ggtaaatggt agttcattca ttttgaaaca taagaaaaaa acacttacta
     3181 gaaatggtgt gccgttttca gtacaaagcg accatcatgg gaaagtcaag ctggatcaat
     3241 tgcctggtgg aacttatcga atcgtttcag ctgatgatcc taaagttgct gtttcttttg
     3301 gtattagaaa agtaaaacaa gagaagatat acttttttga ggggcgaaaa cttgtaaaag
     3361 agcttcaaaa aaatggtttt tattttaaaa tcaatgaata agtaaggaga gaaaaaatga
     3421 tccgcacaac aacaaaattg tctgccaaag aactggaatc tgttctatcg atttggctgc
     3481 atgctaactt ggattctcat gaattcattc catcaactta ttgggagaat caagtagaat
     3541 ctgttaggaa agctttacca gatgcagtga tttacttagc agaaatagat caagaaatta
     3601 ttggttttgc tggggtgatt gatcagttta tcgcaggaat ctttgtgaaa agggaatacc
     3661 ggcaacaaag aatcggtacg caactaatga aaaaaataaa agaaaccaat aaaacactga
     3721 ctttgacagt ctatcaaaaa aatcaatcag ctattcattt ctatctttca caaggtttta
     3781 caattcaaac acagcaaaaa gatgaagcag taaatgagga ggaacttgtg atgaaatgga
     3841 atcaaaacgc tgatttttga tgaacagaat aacgaaataa tgctcccagc ctaatttttt
     3901 gctgggagca ttatttcatt ttgaagtcac taatttaact gtgtgaatat gcagttgtta
     3961 caatctttga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
     4021 aatatgagga actatttttc catttgtttc tctaatactc aggtcaaaac gctgatttca
     4081 caacctcttg ataaacggtg ttcaaacggc tgcttctcgg taataagccc actagcctag
     4141 aaaatatgaa aagctatttt ctaactagcg ttcttattac tcaaagcata acgctgattt
     4201 cacaacctct tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
     4261 caaaacatga ggaactattt ttccgtttct ttctctaata ctcaggtcag aacgctgatt
     4321 tcacaacctc tctttcacaa ccttatttta ctcgtttata attttcatcg aagtattttc
     4381 cgctacggat atcatctggc attccatcgt atggttcttc gctaataaca tcttcattgt
     4441 ctatacctgc atcacccata taagtgatcg tagcaaattc aggaacaagt aattttggat
     4501 aagtttcata tttttcacga gctgcttcca tttctgcgat atgttcgttt tggaaagtaa
     4561 cagtgatttc tggatgttct tcaacccatt tagggaaaaa gatcattccg tgaagtttct
     4621 tttcatttgg cataaagtcc aatgccacgt ctgtccctgt tggctctgtc caagtgattt
     4681 tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg gtcttttacc caacggcctt
     4741 tgaccattcc gccatggata cggtaatcaa cagtatggtc atttttggca taccattcat
     4801 attcccaacc attgtcatac gtatagataa agtgagttcc aataaaatct tccaattctt
     4861 taaattcttt catgtttaag catctcctta taaattaaaa catgtaataa actacatgtt
     4921 ttcgattact catttatgat agaatggatt taaagatttg tcaaggaggg aaaactgatg
     4981 gcaagaaaaa atacacttcg atatattttg ttagggttat taagtaagaa gaagatgaca
     5041 ggatatgaat tgaatcaatc gttcaaaaat gaaataggag aattttggca agcaaaacat
     5101 agtcagattt atcctgaact tgcaaaaatg gaagagcaag gaatcattca gcatcaagta
     5161 gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat taacagaaga agggaaggaa
     5221 caattggcag agtggattca tacacccacc aatgaactac cggttaatcg agacgaattt
     5281 gttctgaaat tgtatttcgt caaagacgtg aatgatccgg cattagtaga aattattcag
     5341 cagcagcgtg accttcatga agaaaaacga cttcatctat tagagcgaaa aaaaacgatc
     5401 tttcctactg aaaaagaaaa acatgaaaat tacggacact acctgatttt agcgcatgcg
     5461 atcaatcggg aaacagaata tgcaaattgg ctaacagatg tattagaaga aagtaaccga
     5521 aaaaaataaa aaaatatccg aacagtgatg gagaattcct ttctatatga aagaaacgca
     5581 gatcaccgtt cggatattct tatgttcaaa caggcctttt ttcaagtatt tatgtagcat
     5641 ctacttatta aaaagcttgg tacttaggag attagtggcg tttagagttc ttttaacaac
     5701 tgttcgtagt cagctttttc atcgacagga aggagcttgt aagttaattc gtctgtctcg
     5761 ttgtcatgaa tgatgtacat cttagcattc tcttctttga agttaccgat ttgtaaaatc
     5821 gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt ttgaaaaaac tttacggaca
     5881 tggtgcattt tcttttggcg gatttctgcc ttttctgcag cttttgacaa agtaaatccg
     5941 tcatctaaat attttttgat cagttctact tttatgactg taggtaaacg atattttcta
     6001 ggacaattcg catcagcttc gatcgaacga atgaatccct tttgttccca ataccttaat
     6061 tgacgtggag acacacctac gatatcactt aattcactga tgccaacaac caattgatca
     6121 ttttccaaca aacgtttaag ggaagcccca accatttttg tcactccttt tgtcctcttg
     6181 cttcccttaa ttatatccgt attaacttat gtgtcaagta ggttgacaaa tagcgaattt
     6241 aggactaaag tattctagta gtttcagaaa aaattcagaa agaaggagaa atatgagtaa
     6301 caaccaaccc gtagatattt acggaaaacc atataatcgg acacttttag ttgttgtctt
     6361 attaattggt acattttgta ccgttttgaa tcagacctta ttgacgacag cttttcctac
     6421 attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa tggttaacga ctggtttctt
     6481 gctagtcaac gggatcatga tcccaataac agcatggctg atcaataaat tcagttcaag
     6541 aaatttatat ctttctgcca tgacgatctt tttgatcgga acgattactt gctttacagc
     6601 acctaatttt agtacattac taatcggtcg tttgatccaa gcttgcggtg tgggagtatc
     6661 gatgccatta ttgcaaaata tcatgctatc tattttcccg ccagaaaaaa gaggatcagc
     6721 aatggggatg tcagggatcg ttattggact tgctcctgct ttaggcccta cattatctgg
     6781 ttatatcatt gataactacc attggcgtga cttgtttggt atggtactgc caatcgttgt
     6841 tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt attccattgt caaatcctag
     6901 cattgatatt ttatccgcta ttttatcaac gattggattc ggtagtttac tttacggatt
     6961 ttctagtgta ggagatgacg gctggggaag cttgaaagta attggtttct tagttgttgg
     7021 tgtgattttc atcgccttgt tctcatggcg tcaattacat ttggaacatc cattcttgga
     7081 acttcgtgta ttcaaatcat ctacattcac gattgcagct gttttagctg gtgtaacaaa
     7141 tatggcgatg gtaggggcag aaatggtcgt accattgtat attcaaaata ttcgtggaga
     7201 atcagcattc cattctggct tgatgttgtt gccgggtgca ttgatcatgg gggctatgat
     7261 gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa cgtttagcca tcacagggat
     7321 gttcattttg acagcggcaa ctgcaccatt tgctttcttg acaaaaacga ctccagtttt
     7381 atatatcgtg atcctttatg cgatccgtat gtttgggatc tccatggtca tgatgccggt
     7441 aacgacttct ggtatgaatg cattgccaaa taacttgatc agtcatggga cagcggtaaa
     7501 taatacattc cgtcaaatcg caagctcgat tggtacagct attttgatca gtgtgttgac
     7561 gaatgtgaca aaagcaaata tcccaagcaa atctgtattg gataatacgc cattagctta
     7621 taaagatcag gcaatcaatg ccacactgtc aggttaccat gccgcgttta tcgtagcgat
     7681 catctttggg attcttggct ttattattgc ctttttattg aagaaaaaag aacctgttct
     7741 aatgaaagcg ggggataatg catgattatt ggagttttga ttgtcagctt gcttgctttt
     7801 gctttaacga acgtgttcgc taaaaaaaca tggcaaacct ttttatcttt gattttcgga
     7861 ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt tgtccaatca ctttggaatg
     7921 gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca gtgctgattc ccaaggagca
     7981 gatatgcttt tgtataaagc tttagggaat ggaaaagaaa aagtgtatct ttaccgaaca
     8041 aatgaaaaac aagaaaaacc aaaagcaact ggtacggata acgaaacaaa taaagtagaa
     8101 aagacagacg gcgatgctga aaaagtgacg aaaactactt attgggaata caaaaacgac
     8161 atgtataaat tctggttcaa tattgctgat aacaatcacg aatatgacag ccgagtgaat
     8221 acattcaaaa tcccagagac atgggtagaa ttaagtacgg accaagcagc caagttagct
     8281 gaattagtga agaaacagca aagtacgatg gaaagtgaag caaaagctta tgttcaagac
     8341 ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca aagcagagca agaacaacgt
     8401 acgaaagcct tagctgctga attccaacaa caagcattcg caaaattagt gaaagaagca
     8461 aaaggcgaat aatattttga acaccatgta ggaggttgtg aaaaagctgc tctgcatcaa
     8521 gcagtaagaa caatcagata aaaatagcac tccatatttt tcatctgatt gggcttagtg
     8581 ccgtagatgc aagcttttga acaccgttta aggaagaggt tgtgagcctg ccgtttaact
     8641 ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgttgact attgtgactt
     8701 gtgaccgagg agttggcagg agaacaccgt ttattcgaaa aaaggaactg cgacaagact
     8761 tttgtcacag ttccttcttt tttttgctat gctatactag atgtaaaaaa taggagaaat
     8821 aaaatgaggg aaatttattc ttttgaatca aaaatctatg cttccgaagt cggcaagggc
     8881 ggggcatata ttatttttcc gtatgatata agaaaagaat tcggtgctgg aagagttaaa
     8941 gtccaagcaa cttttgacaa tgtgccgtat gaaggcagta tcgtgaatat gggcgtgaaa
     9001 aatccagatg gttcagtctg ctacatcctt ggactacgaa aagatattcg taaaaacatc
     9061 ggaaaagaaa tcggtgatat tgtagcagtt acagtgaagc aaaaataaaa gaggttgtga
     9121 caaaagacgt gtcacaacct cttgataaac ggtgttcaaa agccagtttc tcggtagtaa
     9181 gtcaaattta tgcaaaaatg tggaaaccca tgtttacaaa atttttctta ctactcgaaa
     9241 cgaagcggct ttatcacaac ctcttgataa acggcgttca atcagctgtc cctcggtatt
     9301 agctcaaaaa cggcaaaata tgagaagcta tttttccatt tttttctctc atactcaggt
     9361 cataacgctg atttcacaac ctcttctttc ataatggttt ttcaccatct ttataataaa
     9421 ctggttattt gatatggaaa tctatttttt ccaaccaatc taaaatcttg taacctaact
     9481 cttctgacat cggaaaagag gcgttgattt gtgcattgga gatatggtca acatggatac
     9541 cggaagtgat cgtacaactt cccggaacgg cagttttgat gatcacagct attttttcgg
     9601 ctaagacatc gtctttatgg aaccgaccat cgtggctagg gaaacgtaca gtttgcatct
     9661 ctgtatcacg agaaaatgta gtgactgttc cgatatgtgg tgtatctccg cctgttaaag
     9721 tgatcaataa gtctttaccg attactttaa cagcagcttg gatcgtgaaa cctagtttgg
     9781 ttacttcaac tatcgtttct ttttccatat taatcaccct gccaacgttt tgaatgctca
     9841 tttacaattt caaattgatc caaaagtttc attgtatggt gattaacaag atcttggatc
     9901 gtttgaggct gattataaaa agcaggaatt ggtggtatga tttgcacacc tagtcgtgct
     9961 aatttagtca agttttccaa gtgaatggca tttaatggcg tttcacgagg aacaatgact
    10021 aactttcgtt gctccttcat tgttacatca gcagcacgtg caatcaggtt gtcgcctaaa
    10081 ccaacagaga tacctgcgac tgttttcata cttgccggta cgatgatcat tccatcggtc
    10141 aaaaatgatc cgctggcgac agcagcacct aaatctttgt ttgaataatg gtaatcaaac
    10201 aggctttcga attcagttag tgataagtcg gattccagct ttaagttctg ttttgcccat
    10261 ggactgatga ttccgtgtgt ttctatcatc ggatattctt ttagtttctt cactaggttg
    10321 atggcataaa tggtgcctga agcgccactt acaccaatca cgattttttt cttccgttgt
    10381 gccaattgtt ccattttccg cactcctttt tatgatgtca aaacagttgc agatcattca
    10441 ttatttcaaa tatttttccc aatcttctac ttcttggaat tttgcacgta caaaatgttc
    10501 tttcatatcg aaaggaactg taccgtcaaa gatagttttt gcacttatcc cacggaaacg
    10561 aatagagcca ggattatatt ctggtaattc tgatgggtcc aatggatggt tcctcattcc
    10621 tgggatcacg atgatatctt tatctccttg gaaacgagtg ttgaaagtcc aaactacatc
    10681 gttcatgtcg aaaatatcaa catcttcatc gactaaaatg actgtcttca attctttgaa
    10741 ggctgataaa gcaagaatag ctgcttgacg ctgaatacct tcatccgctt cgttttcttt
    10801 acggatttgc atgatggtca tcaatttacc accacctgca ggaggattgt agacatttac
    10861 tacttttcct ggaatagctt tatcgaccaa ggcaagaata cttgcttctg taggaatacc
    10921 ggccatagat acgtgttctt cactgggacc aatcgttgtt tgcatgattg gattgtcttt
    10981 tcggtgagta actgctttga ctttcactac ttgtacagct ggattagcat ccccagtata
    11041 gccaggaaat tctggcattg cttttcctga gttcgtgttg atatcttctt gcattgtttc
    11101 atttggcatg atttctgctt caatgatgaa ttcagcacga gcaataccca cttcatcgac
    11161 tgctatacca tcaacgattt gaataggttc gttacgcaaa gctcctgcac accatagttc
    11221 attgtagcca agtggagtgg taggtggttc gaaagtcgtg ccaatggcaa ttgctggatc
    11281 taaaccaata ttgatagtga taggcattgg tttattcaag gcttcgaatt gtttttggaa
    11341 gtgaccaatg tggcgtccgc caggcatgat gtacataccg atcgtatctt tatcttctag
    11401 aaccatacga tggatcgtta catcgctcat tgttttttct ggatctgatc ctaagactac
    11461 acccattgtg atgtatggac cagcatcttc aggagtattt gtaggtgcag gcaaaatgtt
    11521 gcgaatatcg aagtctgcat cggtagctag atgaacgact tcttgtgctg gagcgtctgc
    11581 tttgtctact ttcacaggtt tgattggatt ttctacagcg tctttcaaga aacgaccaag
    11641 tgttcgataa tcatggtgca aaatttcacc tacacgttta cgactagcca ttgttccgat
    11701 gttcacacgt gtgccgggga aacctttgat attgttgaaa gtcatagctg gtccttcttg
    11761 agtcggacgt tttactgtac cgccagcacc gatataacgg taaacaccag ataattctgc
    11821 ttcaggatcg atttcaatat ctgtttcgtg atattgacct ggaagtgttt ttagttcttc
    11881 gatgactttt cttaaatcat acggttgatc tgtcatgaga gattcctcct tagttgtctt
    11941 tacctatcta tagtaaaagg tgaaaaagag gaaaacaatt caacttgttc ggtaaagtat
    12001 tctacaatgg aatggatgcg tttttttgat aatagaataa actatgatat acttatcact
    12061 aaaagaaagc gcatacttag tgttggagga aggaaagtga aaaaaatgga ggagtcattt
    12121 cctaaagcag taaaagtgga aaatatagcg aatattttga aagttacatt tgaaaacgga
    12181 gaagtaaaat acgtgaaatc tcattggaca gaagagataa ccgatgcctt gcaatttgga
    12241 aaaaaaggaa gaggaaaaag aaaaaatctt ctcgcacttt ctcgtaatat gtggattggg
    12301 actgaagtaa cgattgaagc agatggaaca gtattcataa atggaaaaga caggtatacg
    12361 ccagaagagt tgtggtacaa aggaaagaaa agtataccag aattataaaa aataattaaa
    12421 aaaaacagcg cgaaacaagg aacatattac ttcctcgttt cttgctgttt tctactattt
    12481 cagcgctctt atgactcaaa tctaaatgtg gttgtcacaa ccttattctg cacgcaaagc
    12541 gatagcggcg tcttttttcg ctgccattcg tgcaggaaga tgaccgccaa gcatcgtcaa
    12601 tatagtagag acgatcacta gaataatcgc atggacagga tttagttgag caacattttt
    12661 tagatccgtc atgttgtata agatgctatt gatagggaac gtagctagcc atgcaataaa
    12721 cacacctaat atcccagaag aaatacctaa aatacacgtt tctgcgtcaa atacgcgagt
    12781 gatgtctttt ttacgtgcac ctaaagcttt tagtacaccg atttcttttg tccgttcaat
    12841 cacagatgta taagtaatga tactgatcat gatcatactt gttactaaag agatccctgc
    12901 aaaagcaata aggacataag tgatagcatc catcaatccg ccagtcagtt cagtcatcgt
    12961 tccagctaaa tcagtataaa tgattttatc ttcatctgat ttgcctttat tgtaatcatc
    13021 tagataatcc aagatctttt ctttgtcttc aaaattgttc ggataaatca tgatacttga
    13081 aggcaagctg tctccaccta aataactaag caatgtttgt ttggcatttt cgtcaacttt
    13141 ttcagtagtt aagacattta caccactgtc tctttgagct ttgacgattt ctgattcttt
    13201 gttttcgttg acgatttgag tcgttaattg atcgctataa gcgattcctg gagataagag
    13261 attcattgta gaagaagatt tcacgcgtag gatccccgag atcttcaatt catcagaagc
    13321 attctgatac atcgcatcat aatctgtatt agggataaaa ttcccagttg gaagttttgt
    13381 atagtaagta ttgttgttta ctaatttgaa tgttgtacca acgatatcat caaaatcaag
    13441 tttttcgtct tctttcacat caaatccaag attcttcaat gcgttgatat tagtattatt
    13501 attgccgtct acgatcagca cgacatctgt tgctgaggct ggataacttc cggcaagtaa
    13561 agaataattg tcttttaaaa agttttcttt gctagtgtca agctgtgtag ggaaacttga
    13621 cactcccaca cctgtcattg ctgacatcgt actagataaa gaaagagatt ccgtatcagg
    13681 attttgattc gagaagctga caggctgtac tttgccattt acatcacgta aaagattgat
    13741 tcctgtagta cgggtaaatc cgatattatt acttaagtta ggatcgatgt ctgttacgta
    13801 atcgacgtat gtttgatcaa tcttattcgt gtgctgtgct ttatcttctt cgctgacttt
    13861 tgcagtaatc gttttgctgt ctggataatc cgctttgctt gcacccaatc cggcatcgtc
    13921 acgagtctgg ttagtcgtta ccttagaaat agtaatcgga aatttcgcca tcgtttcagc
    13981 ttgcgtgtta tcgatttgtt tttggaatcc agaagaaagg gacagaacga tcgcgattcc
    14041 gatgatccca atactggaag caaaggaggt caagaaagta cgtccttttt ttgttcggat
    14101 attgttgaag gaaagcttta acgctgtcca aaaactcatc tttgttctgc gtaagttaaa
    14161 ttgatcatct tttggtcgtt cgatgtgagg atgggaatct gttaagatct taccatcaga
    14221 aaattcaatg attcgatctg cgtattgatg ggccagttca ggattatgtg tgaccatgat
    14281 cactaatttt tctttcgata actcttggat caattccatg atctggatac ttgtttctgt
    14341 atctaacgct ccggttggtt catcgcacaa aaggatatct gggtcattgg ctagtgctcg
    14401 agcgatggcc actcgctgca tttgtccacc agataactga tttggctttt tatgcatatg
    14461 atttgtcaag ccgactcggt gcaatgcatc ttctgcccgt tttcgttttt cgtctttaga
    14521 aacaccactt aatgtcatcc caagttcaac gttttcaata atgcctagat gaccaatcaa
    14581 gttgtaactt tgaaaaatga aaccgataga gttgtttcgg taggcatccc agtcactgtc
    14641 cttaaagtct ttagtagact ttccgttgat taccatatca ccggaatcat aattatctag
    14701 tccaccaatc acattcagca tagtcgtttt accagaacca ctaggaccta aaatcgctac
    14761 gaattctttt tgtctgaaag cgacagacac accatccaat gctttggtgg tggtttcgcc
    14821 gactttataa tattttttta tatcttttaa ttgaagcata atcacccttc tcccgtattg
    14881 aaatattcgg tatactaata agtatatcag aacaatgatc gtagtatacg aaataaatgt
    14941 taactgaaga tgaactacaa gaaaaaatct agaaattttt caaaaaacag agggagatca
    15001 atgaatcgta ttttagtagt agaagatgat ccgcaattac agttgcttta ccgttctgtg
    15061 ttggaacgag cgggcttttc agtcatcacc gcattgaatg gagaggatgc gctgaaaaaa
    15121 ctagaagcga cacgtataga attaatcatt acagatatca tgatgccgaa aatggatggc
    15181 tatgacttgc tagactcatt acgtagcacc agatttgaca cacctgttct aatgattacg
    15241 gcaaaggccg attttgagga taagaaacgc gggttcaaat taggagcaga tgattatatg
    15301 acaaaaccca tcgatgtcaa tgagatgatc ctacgtgtgg aagcattgct tcgacgttca
    15361 aagatcaatt acagcagtag cttgataatc ggacaaacaa aattagagct agatgcttat
    15421 acagtgttac aagatcagca ttcgttgatt ctgcctcaaa aagaatttct gcttctgtat
    15481 aaattactgt cttatcccaa caagatcttc actcgccaac agttgatgga tgatatttgg
    15541 ggattagata cgaatacaga agaacgaacg atcgatgtac atatcaaacg tttaaggacc
    15601 cgttttgaaa agacggaaga ttttcaaatc atcacagtac ggggactagg ctataaggcg
    15661 gttatttaaa gatgaaaaaa aagctatttt cacaattatg gatctatttt gctagtattg
    15721 tcttcatttc gatcttagtg acactgctgt gttttctcgg ttttattttc ttattgtctc
    15781 gacattctat ctcgcctgct gaatcggctg gaaaaccaac gttatacccg ttattcgtct
    15841 ttgcggggtt cagtatggtc gtgggtacag gaatatcgat ttttgttggg cggcgtatcc
    15901 tgcatccaat cagtgcgcta gggacaaata tgagtctggt agcaacggga gatttttcga
    15961 ttcgaatgga tgaacagcag aaagtagcag aagtacagca actgtataaa gatttcaacg
    16021 ttatggtcca agaactcaat agtatcgaga cactgaggaa tgactttgtg tccagtgtat
    16081 cccatgaatt caaaacacca ctagcaacaa tccaaggata tgttcaattg ttacaagccc
    16141 caaatctctc tgacgaagaa cgccaaatat ttttatatcg gattatcgaa agtatcacgc
    16201 agctttcaca attaacagag aacatcttaa aattgaacaa actggaaaac cagcgtatcc
    16261 aactggaaaa gaaagagtat cgattagatg aacaaatcag agaagtaatc gttttttttc
    16321 agcctaaatg ggagaaagaa cagctagaat tggatatcga acttgctgca gtaaattaca
    16381 caggcaatga agagtttttg taccaagtgt ggctgaatat catggataac gccataaagt
    16441 acaatcaaat caatggacag attcatatca aactatttga gacagccaca gagattgtac
    16501 tggaagtgac cgattcaggt gtaggaatga atgaagagac cagagatcga atgtttgaaa
    16561 aattttatca aggagataca agtcgtcaaa tttctgggaa tgggctagga ctttcactgg
    16621 ttaaaaaaat tctagaactg catgatggac gtattgatta tagcagtatc gaaggagtag
    16681 gaacgacagc aatgatacgg ttaagtaaac aataggaaaa agaataaaac ccctaaaaat
    16741 tctattgaag gtttttaggg gtttttagtc gtttttttgt gattagggaa atgttcttac
    16801 ggttcttttg gcataaaaat cgctgctttc ttattgaact acgagaaatg ttagaggtgt
    16861 cattgaaaca aggacatgat gcaagaaaga gagcgaaagt catgcgtcgc tctctttttt
    16921 tgggtggata gatggacaga aaatgtaggg cttagttttc ttcttgattt aaatactccc
    16981 aaaatgtatc tgaagccaaa gaatgctcac cgactttacg agtaaatgca agataactct
    17041 ctgttgttgg agaaataggg agaatcacag tattttcttt taatagattc ttgactgttt
    17101 tttccataac aatagcaatc cccatgtcat ttgcaatcat attgatgatc aagtcgattc
    17161 ttgctccttt ataagagatt tttggttcaa atccagcttc gcgacacaga tctagtacag
    17221 gttgatacaa gttcgtagtg gggccaagga gcaaaaagtt ctcctttttc agttcgctta
    17281 gatccaattt ctttttacta gctaaaggat gatttttagg tagaactgct acaaaatcgt
    17341 ctgtttcagt aggcaaccac tcaatcgaac ttggtggggc ctggaaactt ctagcaaagt
    17401 ggatcgtgtc actatgttca ttggcgtcat tgtaagttaa ttcgacactt tccacttctt
    17461 tcaactggac tgtaaattca ggatggcgtt tcaaaaactc ggtgattttg gaaaaacctt
    17521 tataattgat cattgtcggt atcgttaaga tatttaatga aagattgttg tctttcgtat
    17581 aggtttcaag cacttcttgc atagcatggt attgttctac gacatttttt acatatggga
    17641 gcaataagct tccagcttcg gtcaattcga tttttctatg ggcgcgttcg aataatttga
    17701 cgtctaattc tgtttctaac gaaagaatct gtttagaaat atttccttgg gtggtgaatt
    17761 gtcgatcagc agttttggtg aaattcattg tttcggctaa atcaagaaaa acttcgaatt
    17821 tatggatatc catcatattc ctcctactta ttccaataaa gaatacttta acacaaaaaa
    17881 tgaattggtg taatcttctc tgtctgttac aatctttctt gcagcgaaag caataattac
    17941 agaaattttt ctgatttttt tcgcttcgta gaaaaagaga ggagtttttt gatgccaact
    18001 ggagttttga tcaatgtagg ttccgttttg tttggcggac taattggcgg attagtagga
    18061 aataaattaa gtgaacattt taaagcacag ctgacgatgg tttttggtgt gtgttcgatg
    18121 gggatgggaa tctattccat tgcgccgatg aaaaatatgc cggctgttat ttttgcttta
    18181 gtgataggaa cagcgattgg tttgattgtt catttaggta atgggataaa taagggggct
    18241 gcgctgatgc aagtacctat ttctaaaatc ttccctagtg aaaagcttgg tatgacccac
    18301 gatgaattta tcagcacatt agtgacggtc attgttctat tttgtgcaag tggtacgggg
    18361 atctatggaa gtttagattc tgggatgaca ggagacagta cgattttgat ttctaaatct
    18421 gtccttgatt ttttcacagc agctattttt gcgtgtaatt tagggtatgt cgtttctgtg
    18481 atagcaattc cccaattcat tattttttat attttgttct tattggcaaa atttatttat
    18541 ccattgacta caccagacat gatcttagat tttaaagctt gcggcggatt cttgatggtc
    18601 gctaccggtt ttagaatgat caacgtgaaa atgttcccag tagctgacat gatacctgca
    18661 atgatcgtta tcatgccact gagctggatg tggacaaatt ggattatgcc actgttataa
    18721 taaaaatcaa agagaagcca ttttttgctg actaaaaagg atcaaatctt ttagtcagtt
    18781 tttttgtatt tgacaaactg attttgaaac tatattatat gttgtataat atagattgaa
    18841 ataaaatatt acacttgaga atagaaggtg acgtaatgca catccagatt ccaactgttc
    18901 ttcttgatgg tactgtgcta gcagcattaa gcaaggaaga tatgtatggg tatgccttaa
    18961 caaaaatcgt acaagaaagt ctctctgtca gtgaatctac actatatccg gttttgagaa
    19021 gattgaaaaa agaaggatat cttgagacct atgatgaacc ttttgaaggc agaaaccgtc
    19081 gatattactc gttgacacca atgggaagtc gtcatttaga agaaattatt gatgaatgga
    19141 ttgtttttcg agattcttta gaccggttat tggaggagag cacaaatgaa tgaatatttg
    19201 aatgaagttg tggatcatct tcaagaaatt cctgaagacg atcgttttga tttgatccag
    19261 tattatgagg aatatttttt agattctgga aaaacattag atcagatcat tgatgaatat
    19321 ggtacaccaa agcagtttgc cttaaaatta aaaatcagtt acttttcaga agaagaacaa
    19381 gcagaaaacg agttagtgaa aaatccaaaa agacaaattc gcctgatttg gttaatcatt
    19441 atcggtttat ttgcttctcc tgtcctcatt ccactagcac tggggtttat attggttatt
    19501 gggggactat tggttgcttt actagcgctc atttttagta tctatgtttt atgtatcagt
    19561 atcttaggtg ttggcctatt catgatcatc tcaggaatca gtgtgtttgg acaatcgatt
    19621 ctgagtggat tattctttat cggtgtcggt ctgttcgcca caggaagcgc agtatttatt
    19681 tcgccactgt tgctgaaagg aacaaaaggg cttttttatg tcatgatgaa ctttgttaaa
    19741 tggatgggac gacgttttat tacaaaaaga gatctgcatt cagcgggcgt ttgaaggagg
    19801 acacataaat tgagattgaa acattttttg attaccggta ttggtctgat gcttactggt
    19861 ggcctcctag caggtgcaac ttatacatta ggcgctcaaa agagtctgac ttgggataat
    19921 ggtcctcggt tgatcgatat ggttactgag acaaaaaaaa tcaaggatgc aaacaaaatc
    19981 gtcatccatg cagaaaatca aacagtttcg gtcatacgcg gattggattt tgagataaaa
    20041 acgacttatg ataagacaga aaagccagaa ataaaggaga aaaatggcgt gttgactgtg
    20101 aatgcagtca aaaaatcaac aggtgcaatc attagtttag aagacacaag tgcacaaatc
    20161 actattgttg tccctgcaga tcatgaatta tctgaattga caatcgaagg gaataatagt
    20221 gcaattgata tcaacgaagt atcaatcaaa aaagtgaata ctaagacaca tgatggctgg
    20281 gtatcgttca atgaatttac aggaggaaca ataactgcga aagatgaatt aggctcactt
    20341 cgattagatg aggccaatgt ggataacttg atcgtcacag gtgaaaacag caacttccat
    20401 ctagaagaca gtttgatcag caaagaagga aaagtcatcc taaacggtgg caatttatct
    20461 ttgcatgaga tggaaaacag tggctatact atccaaacgt ctggtgaatc ttatattgaa
    20521 aaagactacc agccaataac aaatactttg ttagaacaag gacagaaaaa gctaaagatc
    20581 actgctaaag acacatcgat caatattacg acggatcaag aggacaatga aacagattga
    20641 tcctgtactc aactggatat tttaagaaaa aacagcaaag atccttccaa tctttgctgt
    20701 ttttcatcgc ttaacgcaca taactatagt aaaataagaa agaaaatatc taaaaagaaa
    20761 acagggtgaa agagtatatg aaggtagaag aaggtcaaag agaaaagtta aaaacagaaa
    20821 tggaaagatt acatacttat atcactcaac taagccaaac cttctatgat ccagacaaag
    20881 aaaaagtgat ggttaattac ccgaacaatt cagaaggtcg ccaattagag caagtgtacc
    20941 atgaagtttt caaacattta ttgacggtga agaaagagct ggattattat tctttaccga
    21001 ttatcgatac aggaatattg aagtatgatg ggaagaagga acgatttatc tttaaatctg
    21061 ttcgggaaga tttgcctctt tcagcaggga tggatctaga ggtcttggta gaagactatt
    21121 ttacagaaga aaagcactgg gttcgaacaa aattggatta tttaccgcag gcagccggag
    21181 ggacacaggc aagcggttgg tacatcactg aagacaaaga gttagaacta gaaggtgtaa
    21241 tggcgcgtat tcgaaaaaaa aattaaaaaa gtaacattag atatttattg aaggataaag
    21301 gagcgcagga agtagcatga gagaaaaaag agcattttat atcaatggtt atgcagggat
    21361 tgtcggattg gtcgtattag ctttagttgg attgtttttc ttctatttag gtatgtggca
    21421 agcgaaagtt tgggcacttt ttttaagtat ttttctttgg ttgatcacgt tattgttgtt
    21481 aagttcggca actgtagtca gtcctaatca agcaaaagta attttgtttt ttggacaata
    21541 tttaggtacg attcgagaaa atggcttttt ccttacgata cctttggcac aaaaaatgac
    21601 tgtttcgttg aaagttcgga atttcaatag ttctgtttta aaagtcaatg atttggatgg
    21661 aaaccccatc gaaatctcag cagtagtagt ctttaaagtc gttgatacag caaaggcatt
    21721 attcgatgta gcgtattatc aagattttgt agaaatccaa agtgagacag ccattcggca
    21781 tatcgctagc caatacccgt atgatacatt caatgagaat gatttgacgc ttcgaggaaa
    21841 cacgacggca gtgtctgacg agttgcaaaa agagctgcaa gagcgtttag cagtagcagg
    21901 tgtcgaagta atcgaaacac gcttgaacca tttagcctat gcaacagaaa tcgctagtgc
    21961 catgctccaa cgtcaacaag caaaagccat tttgtctgct cgacaaacca ttgttgaagg
    22021 agctgtcaca atcactcaaa tggccttaga gcaaatcgaa gatggacaag aaatcaattt
    22081 caccgatgat cggaaagtcc aattgatcaa taatttactt gtctcaatca ttacagacaa
    22141 aggaacgcaa cctgttatca atacaggcga catgaaagac cgtaagtgac atactttttg
    22201 taagatagga agaaggagtt taggacggat tattgtcttg aactcttttt ttagttgttt
    22261 taaagaaaat gacaaaattg aaagaagaat gatagttgat tcgaaggcag aattgttcgc
    22321 ttttttctat aatcagtata taaagtaaga gagaagggga aaaagtgaaa aaacttctga
    22381 tcggtctact tgttttcatc gttgtggtac ttgcaggggt gaaaatcgca gattacgtag
    22441 ttatgggtgg agaagaatac tatgtacaaa ttacgacaga tggtacaaaa acagtttcaa
    22501 aatcagactc tggacaagaa acagtcaact atagatatat cctgcctggc tatacgaaag
    22561 agggtaagga aaaagaactg gacttcaagg gacaattgac tcgaccgctt cgtaaaggag
    22621 catatttaaa aatcacctgg aacaaaaata aaggtgtcac ttcgtatgaa gaagtcgaaa
    22681 aaaaagacgt gccgaaaaca gcactggaaa aactaattaa agggtaggga aagaaatgaa
    22741 caaaatagtc gaagtacaga atgtaacaaa agtatacgga aaaaacaatg agaaaaagac
    22801 gcaagcatta agcggcatca gcttcgatgt agaaaaaggc gaatttattg ggatcatggg
    22861 cgcttctggt tctggtaagt cgacactttt gaatatttta tccacattag ataaaccaac
    22921 agatgggcat atccgaatca atcaaaaaga tgtgaccaca ttaaaaggaa atcaactagc
    22981 agattttcgt gcgaatgaga ttggctttat tttccaagat ttcaatttat tagaaagctt
    23041 gacagcacaa gaaaatatcg ctgttcctct ttctttacaa ggcgtcagac caaaagaaat
    23101 caagcaacgt gtccaaaaaa ttgcagaacg tttatccatc tctcatattt tggaaagcta
    23161 tccttctgag atttccggtg gacaaaaaca acgggtcgcc gccgcacgtg cattgatcac
    23221 acaaccaacg attctattag gggatgagcc tacaggtgcg ctggattcca aaagtgcccg
    23281 ggatctttta gatacgatgg acgaattgaa tacaaaagat catgtatcga ttcttcttgt
    23341 gacgcatgat ccgttttctg caagttattg ccaacgtatt ttattcatca aggatggcgg
    23401 tatccatcaa gaagtgaaac gtggagatca aagccgtgat tccttctatc gagaaatact
    23461 atctattctt ggaaacttgg aacagtaggg ggagattgac tatgctttgg aaattatcac
    23521 ttactgggat caagagccgt ttaagagact acttggtgtt attctcagga ttagttatgg
    23581 catctgctat tttttacatg tttgaatcaa tggcaagcaa cgaagctttt ttgaaaagta
    23641 atacgatcgt tgcggctaca gttatcattt tccgattcgg ttctgtttta ctagggatca
    23701 tcacattcgt ttatatttta tatgcaaatt cctttttgat gacgatgcgg caaaaagact
    23761 atgcaatgtt tatgatgctg ggagcaaaag gcagaaaaat cgcacagatg attttttctg
    23821 aaacattctt agtagggatc gcagctactt tagtgggttc agctgtcggt gtaggattga
    23881 caagtatcgt ccaccgatta ttagtggatc agttagatct caatatttcg cattttacac
    23941 cttttagtat tcacgggttg ttgatcactg ttttgttttt tgctgttttg tttttattag
    24001 ctgctgttgt caatgctttt tcgattgtca aaaaaccgat tttagcattg attcgtgcgg
    24061 atcagacacc aacaagaatc aagcagcata aattcttgtt tttccttgaa gtagtactgg
    24121 gaattgcttt tctaggctgc ggttactata tgatgaaaca cgtggggacc tatcaagtct
    24181 ttggtatagg ggtagcctta gttacgatcg tattaggaac ttatttcatt ttccattctg
    24241 tgatcatttt ctttttatca ttactaaaga aatcagaaca gatttcattg aaaaaattga
    24301 acaatttcac attgtcacag ttaaactttc gtattcgtga atatacacaa atgctctcaa
    24361 tggtcgctat gctgtttgcc ttggcactgg gcgctttgac ggtaggatta ggttttaaaa
    24421 atgaaattgc agacttgaca gaaaacaatt ctggatatga tctagtgatg aataatcctc
    24481 gggcagaaga tcagcaaaaa atcaaagaac tttcaccaac actttctgct tcttatactc
    24541 aaaaagaaga tgcgggaacg atttactata atgcaagtga atttgcggct caaccattga
    24601 tcatgataaa acatgaagat ggaacaccgc cgaagatcac gaaagaaaaa gttcctgcta
    24661 aaaaaatgaa tgaactgact gtacaagagg aacttcgaag ctatgaattg ccggaacagc
    24721 aagaaaaaga aatcaaggta gtcagtcaat cagaatttga tcaactgaac ttgccacaat
    24781 ccactttaca gctagttcag gtaaaagact tccaagcaaa cctgaaacca atcgaaacac
    24841 ttgttgctca aaataaaatg aacaatccat ctttgcaaaa agtagagtac agcaaccaga
    24901 aatatgaaat gtatgaagtg tataatggtc tattctctgg ttttgaattt atgggattct
    24961 tcctaggttt agctttcttg accatgcttg ctagctgttt gatgttcaag atcttgtcag
    25021 gaagcaagag tgacattgct cgttatgaaa tgcttgaaaa gataggaaca cgacgcgggt
    25081 tgcttaaaca atcgatccgt agagaagtag gcgtgttatt cttagcacca ggaatcttag
    25141 gtgtagtcca tgtcttattc ggattacaaa tgtttggtct attcatgcaa aatccataca
    25201 aagatatttg ggttccgttt acgatattct tcgtattgta tttcatctac tatgtactga
    25261 cgacatggtt atatactgga atcgtattga aaaagagatt gtgaaaaagc tgtcctgcat
    25321 caagtaataa gaacagccaa gcaaaaatag ctcctcatat ttttcgtttg gttgggctta
    25381 ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa aaagaggttg tgacaatatt
    25441 tttgtcacaa cctttttttc acggtctttt attagaatat agtaaatttt ttctcgttac
    25501 tcacggaata agctgttaag aaaacggttt ccatgtatac tggaatcagt gcaaatgaaa
    25561 gaaaagaggg tgcaaggaac atggaaatca atgaacaaaa ttttggcgaa aactatcact
    25621 tgattacgat cacgaatcaa aatgggacat ctttgtctgt cagtgatctt ggggcacggg
    25681 ttgttcattt tcaaacaata atcgataatg aaaaacgaga gttgatctta ggttttgatg
    25741 aagcaaaaga atatctagaa aaagaccctt atatcggtgc ttcaatcgga cgtatggctg
    25801 gacggattac gaatggcaca ttcaaattag atgatgaaac gttcgaagta aaaacagacc
    25861 cggacactgg tcattgttta catggtggtg cacctagctt tgaagccaag cagtggcatt
    25921 atacagtcat caatggtgaa aacgaagcat cggtcatttt ctatcagact agcccagata
    25981 tggaaaacgg atttcctgga actttagatg tagaagtacg gtatacgctg acgaacgatg
    26041 atacatggcg agtgacggtc caagggatca gtgaccgcaa aacagtattc aatcctacaa
    26101 atcatgtcta tttcaattta accggtgatc cagcacagtc aatcgacgaa catatacttt
    26161 ggttgaatag ccgattattt gctacgctca ataaagatac aacgcctaca ggagaagtag
    26221 tttcagtaag tggcacagca tttgacttcc aacgtcctaa aaaattaggt gaggtctttg
    26281 ccagtgattt tcgccaatca atgatagtgg gcggcatcga tcatccattt ttgttgaaaa
    26341 aacaatctgg tttactaaat gctgcggaat tgacaagccc agatgaaaaa gtatcgatcc
    26401 aagtaaaaac agatgcttca ggaatcgtga tttttacagc aaattttgga gaagatggac
    26461 cggaaatgcg cgggaataaa ttagtaaatc atggcggtat cacatttgag actcaagaac
    26521 tgccaggagc ggagcgactt gaaagttttg gagatgtcac tctggaacct aaccaagtac
    26581 gcgagtttgt aacggaatac aaaataaata taaagaaata gaataaatta aa
//
LOCUS       DAZUKT010000039        26160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000039 DAZUKT010000000
VERSION     DAZUKT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.039"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG816_001550"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG816_001550"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4920430.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACG816_001551"
     CDS             1286..2461
                     /locus_tag="ACG816_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4920431.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACG816_001552"
     CDS             2465..3106
                     /locus_tag="ACG816_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920432.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACG816_001553"
     CDS             3103..4020
                     /locus_tag="ACG816_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4920433.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACG816_001554"
     CDS             4017..4688
                     /locus_tag="ACG816_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920434.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACG816_001555"
     CDS             complement(5229..5717)
                     /locus_tag="ACG816_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4920435.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACG816_001556"
     CDS             complement(5918..6184)
                     /locus_tag="ACG816_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4920436.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACG816_001557"
     CDS             complement(6184..6447)
                     /locus_tag="ACG816_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4920437.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACG816_001558"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACG816_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACG816_001559"
     CDS             complement(7250..9073)
                     /locus_tag="ACG816_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4920438.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="ACG816_001560"
     CDS             complement(9671..12376)
                     /locus_tag="ACG816_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4920439.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="ACG816_001561"
     CDS             complement(12682..13629)
                     /locus_tag="ACG816_001561"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4920440.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG816_001562"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG816_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4920441.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="ACG816_001563"
     CDS             complement(14264..14626)
                     /locus_tag="ACG816_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4920442.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG816_001564"
     CDS             complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG816_001564"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4920443.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG816_001565"
     CDS             complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG816_001565"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4920444.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="ACG816_001566"
     CDS             complement(16254..17765)
                     /locus_tag="ACG816_001566"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4920445.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG816_001567"
     CDS             complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG816_001567"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4920446.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="ACG816_001568"
     CDS             complement(19592..20668)
                     /locus_tag="ACG816_001568"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4920447.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="ACG816_001569"
     CDS             20864..22609
                     /locus_tag="ACG816_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4920448.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="ACG816_001570"
     CDS             complement(22889..23326)
                     /locus_tag="ACG816_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4920449.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="ACG816_001571"
     CDS             complement(23310..24683)
                     /locus_tag="ACG816_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920450.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="ACG816_001572"
     CDS             complement(25005..25493)
                     /locus_tag="ACG816_001572"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4920451.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="ACG816_001573"
     CDS             complement(25515..26117)
                     /locus_tag="ACG816_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4920452.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8258 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAZUKT010000040        25634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000040 DAZUKT010000000
VERSION     DAZUKT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.040"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACG816_001574"
     CDS             131..3658
                     /locus_tag="ACG816_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4920453.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACG816_001575"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACG816_001575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4920454.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACG816_001576"
     CDS             7446..7787
                     /locus_tag="ACG816_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4920455.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACG816_001577"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACG816_001577"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4920456.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACG816_001578"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACG816_001578"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4920457.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG816_001579"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG816_001579"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4920458.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="ACG816_001580"
     CDS             12998..13735
                     /locus_tag="ACG816_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4920459.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="ACG816_001581"
     CDS             13749..14564
                     /locus_tag="ACG816_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4920460.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="ACG816_001582"
     CDS             14577..15224
                     /locus_tag="ACG816_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4920461.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="ACG816_001583"
     CDS             15240..15896
                     /locus_tag="ACG816_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4920462.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="ACG816_001584"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="ACG816_001584"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4920463.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="ACG816_001585"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="ACG816_001585"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4920464.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="ACG816_001586"
     CDS             18238..18756
                     /locus_tag="ACG816_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4920465.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG816_001587"
     CDS             18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG816_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4920466.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="ACG816_001588"
     CDS             complement(19643..20563)
                     /locus_tag="ACG816_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4920467.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20689..21741
                     /locus_tag="ACG816_001589"
     CDS             20689..21741
                     /locus_tag="ACG816_001589"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4920468.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACG816_001590"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACG816_001590"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4920469.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22826..23266
                     /gene="fabZ"
                     /locus_tag="ACG816_001591"
     CDS             22826..23266
                     /gene="fabZ"
                     /locus_tag="ACG816_001591"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4920470.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23381..24142)
                     /locus_tag="ACG816_001592"
     CDS             complement(23381..24142)
                     /locus_tag="ACG816_001592"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4920471.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24331..25425
                     /locus_tag="ACG816_001593"
     CDS             24331..25425
                     /locus_tag="ACG816_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4920472.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25511..>25634)
                     /locus_tag="ACG816_001594"
     CDS             complement(25511..>25634)
                     /locus_tag="ACG816_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4920473.1"
                     /translation="TFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8491 a         4440 c         5407 g         7296 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa
    22741 aaaagattgc taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca
    22801 attgcctgaa agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga
    22861 attgattcct aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg
    22921 aaaaaagatc gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt
    22981 tcctgacaat ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc
    23041 gatacttatc ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa
    23101 taaggccaaa ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat
    23161 caccaaaatg aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa
    23221 agtctgtgag tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat
    23281 atagcaaaac aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta
    23341 gcatacgttg tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt
    23401 gcagtatatt gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt
    23461 cgatatttgc aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat
    23521 aatggccatc tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat
    23581 agtcgtcttt taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc
    23641 tgccgttact gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa
    23701 ggtggttgtt gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat
    23761 cgcctttagg atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc
    23821 cgttgtagta accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt
    23881 cgggaaataa tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat
    23941 tttcggcgac atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg
    24001 cagtcgtgct ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag
    24061 tcgtgttgtt tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta
    24121 aaataacttt atttttcttc ataactgttc ctttccaatt tactattttg ttatctttaa
    24181 tgataacaaa atagagggaa aatacctact taaattattg gaatttatca tttattttaa
    24241 aagaaaaaga aaaggtgtag accatttact ttgtgtttgc tataatgtaa gtggatacaa
    24301 aaacgaaaag atgcaggagg aaatggtaat atgggaaaat taggaatctc gatttatcca
    24361 gaacggtcaa catttgaaaa ggacaaagcg tacttggatt tagctcataa gtatgggtat
    24421 aaacgggttt ttaccagttt gctgcagatc aatgatgata aagaaaaagt tctttcagaa
    24481 tttaaagaag tagttgatta cgcgaatagc ctaggcatgg aagtaatggt tgatatcaat
    24541 ccagcgttat ttgaacagtt ggagatttct tacgatgact tatctttttt ccataagatg
    24601 ggtgcctacg gggtccgctt agatatcgga ttcacaggag cagaagaagc aaaaatgacg
    24661 cgcaatcctt ttggtatcaa aatcgagatc aatatgagtt cgggcacgaa ttacgttgac
    24721 aacatcatga gttattcgcc aaacacggat aatttattag gttctcataa tttttatcct
    24781 catcgatact ctggtttagg atacgaacac tttgtttttt gttcagaaaa attcagaaaa
    24841 tataatttga atacgatggc attcgtcaat tcgcaatctg ctgagtttgg cccttggcct
    24901 acgcaagatg gcttgtgcac gctggaagac caccgagatc ttgaaattgc gacacaagtg
    24961 aagcatttaa tccttacagg attgattgat gatatttcta ttggaaatgc gtacgcaagt
    25021 gaagaagaat tgaaagaaat ggcagaagca tttaacgcgg attatccaac attgaaagta
    25081 gatacggaag aaggtattac agaaaacgaa cgaatctgct tgtttgataa ccttcatagt
    25141 tatcgagggg atcgctcaga atatatcctt cgttcaacga tgactcgtgt atactacaaa
    25201 gataaagatt tcccgcctca taatacgcga gatatgcatc atggggatgt actgattgac
    25261 aatgaagggt acggtcaata caaaggagaa acacaaatcg ccttgaagga tatgaaaaac
    25321 gacggacgtg tcaatgttgt aggaagaatc tccgatgacg aattattcct ccttgacttt
    25381 ttgaaaccat ggtcaagctt taaattaatt gaaaataaaa aatagataat tagttattgc
    25441 agaaataatc cgctagaaat agccagttat ttctgctttt ttattttgtt tttttatagg
    25501 ttatgatttg tcaaattaag ctagaataaa atgcttcttg tacatagctc aaaaatattt
    25561 cgattggtgt tctgtaattc aatgattttc ttggaatatg attacgttga ttgctgacac
    25621 tggaaataaa tgtc
//
LOCUS       DAZUKT010000041        24996 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000041 DAZUKT010000000
VERSION     DAZUKT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.041"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..446
                     /locus_tag="ACG816_001595"
                     /pseudo
     CDS             1..446
                     /locus_tag="ACG816_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
     gene            538..1068
                     /locus_tag="ACG816_001596"
     CDS             538..1068
                     /locus_tag="ACG816_001596"
                     /inference="COORDINATES: protein motif:HMM:NF019106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFS4920474.1"
                     /translation="MNELWMDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIM
                     KPSDNGKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVEN
                     LEWLSSDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRH
                     TVLNIASGKTFSFLED"
     gene            1173..2003
                     /locus_tag="ACG816_001597"
     CDS             1173..2003
                     /locus_tag="ACG816_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4920475.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            2020..2871
                     /locus_tag="ACG816_001598"
     CDS             2020..2871
                     /locus_tag="ACG816_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4920476.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2868..3227
                     /locus_tag="ACG816_001599"
     CDS             2868..3227
                     /locus_tag="ACG816_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4920477.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3242..3403
                     /locus_tag="ACG816_001600"
     CDS             3242..3403
                     /locus_tag="ACG816_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4920478.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3400..3705
                     /locus_tag="ACG816_001601"
     CDS             3400..3705
                     /locus_tag="ACG816_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920479.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3705..4022
                     /locus_tag="ACG816_001602"
     CDS             3705..4022
                     /locus_tag="ACG816_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4920480.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            4019..4414
                     /locus_tag="ACG816_001603"
     CDS             4019..4414
                     /locus_tag="ACG816_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4920481.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4411..4749
                     /locus_tag="ACG816_001604"
     CDS             4411..4749
                     /locus_tag="ACG816_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920482.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4746..5114
                     /locus_tag="ACG816_001605"
     CDS             4746..5114
                     /locus_tag="ACG816_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920483.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5111..5416
                     /locus_tag="ACG816_001606"
     CDS             5111..5416
                     /locus_tag="ACG816_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920484.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5440..5643
                     /locus_tag="ACG816_001607"
     CDS             5440..5643
                     /locus_tag="ACG816_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS4920485.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5640..5936
                     /locus_tag="ACG816_001608"
     CDS             5640..5936
                     /locus_tag="ACG816_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920486.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            6012..6428
                     /locus_tag="ACG816_001609"
     CDS             6012..6428
                     /locus_tag="ACG816_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4920487.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7143..7310
                     /locus_tag="ACG816_001610"
     CDS             7143..7310
                     /locus_tag="ACG816_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920488.1"
                     /translation="MYRPQYLEQKHEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKY"
     gene            7336..7680
                     /locus_tag="ACG816_001611"
     CDS             7336..7680
                     /locus_tag="ACG816_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4920489.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            7685..7966
                     /locus_tag="ACG816_001612"
     CDS             7685..7966
                     /locus_tag="ACG816_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920490.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            8069..8383
                     /locus_tag="ACG816_001613"
     CDS             8069..8383
                     /locus_tag="ACG816_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4920491.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            8361..10055
                     /locus_tag="ACG816_001614"
     CDS             8361..10055
                     /locus_tag="ACG816_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4920492.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            10075..11253
                     /locus_tag="ACG816_001615"
     CDS             10075..11253
                     /locus_tag="ACG816_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4920493.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            11216..11902
                     /locus_tag="ACG816_001616"
     CDS             11216..11902
                     /locus_tag="ACG816_001616"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4920494.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            11902..13062
                     /locus_tag="ACG816_001617"
     CDS             11902..13062
                     /locus_tag="ACG816_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4920495.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            13072..13947
                     /locus_tag="ACG816_001618"
     CDS             13072..13947
                     /locus_tag="ACG816_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920496.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            13944..14255
                     /locus_tag="ACG816_001619"
     CDS             13944..14255
                     /locus_tag="ACG816_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920497.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            14245..14598
                     /locus_tag="ACG816_001620"
     CDS             14245..14598
                     /locus_tag="ACG816_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4920498.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            14588..14989
                     /locus_tag="ACG816_001621"
     CDS             14588..14989
                     /locus_tag="ACG816_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920499.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            14982..15386
                     /locus_tag="ACG816_001622"
     CDS             14982..15386
                     /locus_tag="ACG816_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920500.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            15398..16006
                     /locus_tag="ACG816_001623"
     CDS             15398..16006
                     /locus_tag="ACG816_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4920501.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            16026..16388
                     /locus_tag="ACG816_001624"
     CDS             16026..16388
                     /locus_tag="ACG816_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920502.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            16391..16573
                     /locus_tag="ACG816_001625"
     CDS             16391..16573
                     /locus_tag="ACG816_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920503.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            16591..19923
                     /locus_tag="ACG816_001626"
     CDS             16591..19923
                     /locus_tag="ACG816_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4920504.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            19974..20711
                     /locus_tag="ACG816_001627"
     CDS             19974..20711
                     /locus_tag="ACG816_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4920505.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            20721..23012
                     /locus_tag="ACG816_001628"
     CDS             20721..23012
                     /locus_tag="ACG816_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4920506.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            23036..>24996
                     /locus_tag="ACG816_001629"
     CDS             23036..>24996
                     /locus_tag="ACG816_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920507.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGS"
BASE COUNT         8970 a         3731 c         5378 g         6917 t
ORIGIN      
        1 gttttattga tcctagacgt ttcactgtac agcaacgaaa cttcatctat gcgctgatag
       61 gcgatatttt catcgataca ggcatgccaa cggacttctg gaaggaattc ttctacttcc
      121 gtttcgaagg tgtcacaggg cgcaaaataa gcctcaaaga tgaatcgagc acaaccgtaa
      181 gtgatgccaa tatcttagcg aatatcatcc tagatttcat ctttgagcat gatattccgt
      241 ttaaaaatgg atatgaaatt ttgccccaaa acgaacagta tttcttttac aaatgcctca
      301 cgaacagggt gtgctgtagt tgcggaaaga aaaatgctga tatacatcac gtagactctg
      361 tcggcatggg aaataaccga aaaaaaataa acaattctgg taggcgcttt atggctctgt
      421 gcagagaatg ccatacgaaa attcatgtcg aaggttttac tacatttaca actaaaagaa
      481 agcttacggc agttgttctc aaagacagtg atttaaaaag attggggttg aatatcattg
      541 aatgaattat ggatggatat cgaaggatac gagggagtat atcaagttag tagcttagga
      601 agagttaaat ctaatcgccc taactatgta attaataagg gatacgttaa caaagaagga
      661 tttattatga aaccatctga caatgggaaa gggtatcaaa ttatcttctt aagcggcaaa
      721 ggttttagag atagaaggta tgtccatcgg ctagtagctt ttcacttttt aaaagaagcg
      781 tatttcgaag gtgctgaagt caatcataag aacggagata aatctaacaa tactgttgaa
      841 aacctagaat ggctcagttc tgatgataat aaaaagcatg ctagaaaaaa tggattaact
      901 aacttgaaag gaccatcaaa actaactgat ttgcaagctt tagctatcaa gcgtttatat
      961 acaaacaaat tgatgagctc cggagaaata atgaaacttt ttaatgtcag caggcatacc
     1021 gtgctgaata tagcttcagg gaagacattt agtttcttgg aagattaaga gaaacacaaa
     1081 ataggtgtga ctaatttcaa aaataaatat caaatcaaag ggatcaagtt aaaccaggaa
     1141 acaattaaaa agttaaggat aggaggataa atttggctga gataagttgg atcaaactta
     1201 aaactactat gtttgacgat gaaaaaatac gattaatcca agctgttcct gagtcggatg
     1261 ccatcatcgt tatatggatt cgattactag ttttagcagg aaagactaac gacgatggtc
     1321 tgatatatat ccagaggaac atgccttata ccgaagaaat gcttgctaca ttgtttggca
     1381 aaaacgtaaa tacggttcgc ttagcgctaa ctacattggc aaatttcaac atgattgatc
     1441 taagcagtga tggactaatt gccatcagta attgggaaaa acatcaaaat atcgagggta
     1501 tggataaagt aaggctaaaa aatgctgaaa gaaaccgtaa atacagggaa agaaagagac
     1561 aggaacgtct caaattggaa aatgacgtta gcgtgacgtc acgtgacggt acagataaag
     1621 atatagaaga agataaagat atagataaag aagaaaagaa aggtaagtat tctgacgaac
     1681 acttacgcct tgctaaaaag ttgcaaagta atttaactga agattttcca aaagaaatga
     1741 acaaagtaga tatcgaaaaa tgggcagaca caatcaggtt gatggaagaa agagataaag
     1801 catctataga agcgattgag tatgtgatca attggctacc tacaaatgaa ttttggtttg
     1861 gaaatattag aagtgctaag aaattgagag aaaaatttga gaagctcaaa ttcgaaatca
     1921 aagcagacaa gaagaatcat aaaaagcaaa gtcaaaaact acagtacagc aatcctagtg
     1981 aatatgacga cttgccaatt taaaaaggag atgcatcaca tggaaagcct agcaaatgct
     2041 atggagaaac taataagaag agtattagtg caaagcggaa aatgtccaga atgtagcgaa
     2101 cctttgtata gttggcgagc taaaaataag gatggttcag aacgttgtaa accaacatgc
     2161 atgagttgtg gttataaagc gttacgtgtg aaagaggata tacagaccga acggatatat
     2221 aacgatagct taaaagcacg agcgttgagt ttttttcaaa atggttcggt attaacagat
     2281 aaaactttgt ttaaatgcaa aatggaaaat tatcacgtag tggatcaaga aacaaaaatc
     2341 gctttagaaa aagcaaaaag ctatacgaat gaggttctgc tgaaccatcc tgcacatttc
     2401 attctatcag ggaaatcagg aagcggaaaa agccacttat caatggccac agcttgggaa
     2461 atacttgagc gctcaaatta tgacaagaaa atacttttta taagctatca agaattatta
     2521 gagcaaataa agttttctta taacaatact gaactgagaa aagaaattga aggatcgctt
     2581 atagccgata ttaaaacaac tgatttggtg gtttttgacg atattggagc tgaattaggt
     2641 agtggggtat caaatagtag gcagtttaca aacaacacgt taaacacgct cttagaagcc
     2701 agacagaaca aggcaacgat catcacaaca aacttatctg gtcctgaact aagagaagcc
     2761 tacggtgaaa gaattgtttc taggatattt aagaattcag aaggttatgc gctgaaattc
     2821 caacaaacag cagacaagcg cataaaacca gtgaaaggta gtatcgcatg aataaatacc
     2881 gtaataaaaa aactgttcat cgaggtatca agtttgattc tatcgcagaa gcagagtatt
     2941 acgatctagc cttgtggcaa gctgaagcaa atggctggaa agtaaaactt caggaaagat
     3001 ttgagctgat gccgaaattt gaactagacg gaaagaagta tcgcaagatc gagtatattc
     3061 ccgacttcac attttataaa aacggcaaac ttgtcaaagt cgtagatgtc aaaggaatgc
     3121 agacaaaaga ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gcgccgttga
     3181 tattagctaa aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat
     3241 catgacaaca gaagaagtga ttcaaatgcg tattcgaaac attcagcgtg aaattgacga
     3301 tctagaacga acaaaggcag tgatggtcaa tgaaacggcg agaaaggcaa tcgatttgca
     3361 catagagaac ttaagaaggg aaattcgtag attggaggaa tgagcgtgga taagaaagcg
     3421 acaatgaaac gaattgctga attaaccaag tcagaatctt ggcaggaaga caaagaaata
     3481 gttgcagaag tccaaaagct cggtaaatca atgtggactg aaaagcctaa acggaaaacg
     3541 ccgagaaaaa ttgcaatctg gcatggtgat cgaattctag taacaggtac agctgaacag
     3601 ttatctgaaa ttactggatt aagcaaaaac attatctggg atagagctag gagcttatgg
     3661 attgattcaa aaggacgaca gtttaggtat gtggaggaga gataatggat ctcattacac
     3721 aatacagtga catcatcctc aagaaaatca tgatgaagat tcagaaagac aaaaaatcaa
     3781 aagaacgagc ggaattagtt aagttggaaa tggctgaaac aggagcagga gtgcgaagta
     3841 gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt caaaaagggt
     3901 tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt catcaagatc
     3961 gtttcaagtt tgtaaagaaa tataaagaga tattagacaa atacatggag gacagcaaat
     4021 gatactgaag tacagggcat ggaataagaa aactcagtca tttattgatt atggtgattt
     4081 ggttttagat ttgagaagcg gaaaaattta cgctggggac attgggttag tggaaagcac
     4141 cattgatgtg actgaccaaa ttgaactcat gcaatccaca ggaatgaaag ataagaatgg
     4201 tgtggagata tttgaagggg atgtagtaat tacatcaaaa ggagcgattg gatatgttgc
     4261 gtatcttcaa caagaagccg gctttgtagt tgtactaaag aaaagtgatt atagattagg
     4321 ccataggaat actggcgaaa gttatgatgt agcaactgct catgaagtca tcggaaatat
     4381 atacgagaat agcgagttat tggaggtaga gtgattatct tagctactga ctacatcgat
     4441 tctttaaaag atgaaaatgg gaatgtccca gcattgctaa attgtggaat taaaagcaag
     4501 aatagaatca ttttgttacg gatatcggct gatttagcaa aatatcttta tctgagcaag
     4561 acagcagtat attacactgc ggctacacgt tatcagtaca agggaaaaac aatcagtcag
     4621 gattattatg atcgtttaat cagtcctgac atgcatggaa aatcaaaatc agcgatcaag
     4681 ttatttggcg caatagagat atatgccgat gattttccaa atttatggtt gaaggaggaa
     4741 gcggaatgaa cgaggaaata aaaaaatcta ttcgttacat tgatagagcg ttatcgcaac
     4801 tcgaaccctt gcgaattgaa agtcataaag gaatgagtgt ctacccaaat cagtatgaca
     4861 aaatgaacgc tgatttacgt aatgcaaagc tggccttaat taacattaaa aataacaaag
     4921 gctgccagta ttgcaaacac agcgaaccgt tgaatgatac cagcaactct aactttgcaa
     4981 ctgcaattga gtgtgaggaa aatggatact acattgcaac tgagtatgac gatggcgatt
     5041 atgcagataa tggctattct gcttatatcc aatattgccc tatgtgcggt cgaaaattgg
     5101 aggtagcgga atgaatgttc aagtttattt aaaaagaaat ttggccaggt ttcctcgaag
     5161 ctgggataca aattcccacc catccgaggc agaagaagag agctatctaa tgtctgctaa
     5221 catgatagaa attacaagag atgatgcgga tgagtttgat aaaaaaagat catggtactc
     5281 ggtttcgaat ccaatgtata cggctgtaat agaagattat catatatccg acagatttat
     5341 tctggttgat ctagaaagac ctaagaaacg aattaaacag tatagacgtt gcgggttttc
     5401 aacaaagaaa tggtgaaaac aaaacagagg tgactcagaa tgaacaacag acatcgcaga
     5461 ataacaaaac taagaaaaca ggaactgaat gtactaaaga caaagtttga aaaagaatac
     5521 ggagtttctg cagaagaaac atataaagtg gcaagtcagt gtgttgctga tgcaagtgat
     5581 gctattcgta agtttggaat ttcgatatta agtgatgatc gtaaatggga ggaaaaagaa
     5641 tgaaactaaa agacggattt tacgctagta gccacggtat cggcggttta atgctggata
     5701 tgcagacaaa gaaccctaaa acacgtaaga aaccaaaatt caaagtcggt gacatggttc
     5761 gctgcgaagc agaagggttc atctatccat ttcgtggata tgtagaacat gtctataatc
     5821 actcagcgat cattcgcatt gaaaacacga tggaatgcga caagttatta gcgaaaagca
     5881 aagagaattt agcagtggtg agattggtgg atattgaact aatcaatgac aaataaaaaa
     5941 gccggatcgc tccgactaac ataataaaac agacaagttt attatatcac ataaaggagc
     6001 ggtttgactt gatgcaattg ttacgagagg tagatttcaa acagacaaga tgtaatgcga
     6061 gagatgtgct gaagaacttt cggcgtttgg agcgtatggc aggtcgctct ttgatagata
     6121 ttaagtcgcc gattataacg gatatgccga aggcgccgaa gcacggcaat aaggcagagg
     6181 acgcgatcat tcagatgatg gatatagaag cagagagaga tgcgattcta gcggctttga
     6241 tggctcttag tctgattagt cgtcagatac tctactacag cttctgcgac gtaaataagc
     6301 attctaatta tgaaataggt caattgatac gaggatacgg agagaagaat gtagagaagt
     6361 tgaaatccat cgcattgatt gaatttgctg aagcatataa aaaaggcgta ttaattcagt
     6421 atcgttgatt ttgtagggtt tttgtaggga tagtgtaggg tttttgagcg gtttaacgtg
     6481 atattatggt agtgtcgaaa gattaggaaa caggacttcg acaaaataaa atgtaaggga
     6541 ggaaatctcc ctcatcgttt aattaagctt cgatagacag cagcggaaat attaagaata
     6601 aggatgtgaa ttttaactcc ttctaaattg ttcttattat ctatcatccg ttgctgtcta
     6661 ttgtcattat gtcactgtgg cggaaagggt agacgcttaa aaataaggtc aatacgtcga
     6721 gggatagcct taacgtttta tgatttgacc atgcaatgtt cgattcattg ccagtgactt
     6781 aaggaaccta cggaaacaat tcatcttatc ggatgccgat gaattggctg actagtcggg
     6841 atgctactag cagttagaag gcataaaata ctagcgcaga cgtgcgccac tctcaggtgt
     6901 aggttaggag agaaacatta gttggggtta ttaggaatac gataacctgc ttgcgacaaa
     6961 gctttgtact gtcgcgttgg tcatgaacag agacggtatt ctgtttcagt attcgttagc
     7021 aaccgaggga tgtggcagtg gtgaggtgca ggaagtatca gacttgtttg tgtgcaagtt
     7081 actattacat attagatcac tctttgagtg gtctttttat tttgcgtaaa ggaggcacga
     7141 cgatgtatag accacaatac ttagaacaga agcatgaagt aatcactgtg caaaacggta
     7201 acggtgagat agtacgaaag tatagaagac caataaagag cgatacatat aaacggaagg
     7261 aaagcaatga agttattcca ttgtatggca aaagaatagc taagtattaa ataagattgc
     7321 gaaaggagat gggacatgac cgaggaattc tatagatggt tattacagtt gataagagaa
     7381 gatcgtttgg ttaagttcta tcagtctcct aaatggcgca ggcttagaga gaaagcgatg
     7441 aaacgagatc actatgaatg ccaagagtgt agaagactag gtaagtatca tagagtagag
     7501 aacgttcatc atataaagga agtcaaggat agacctgact tagctttaga tttagataat
     7561 cttatttgtt tatgtgttga acatcataat gaagttcatg gcagatatct tacagcgtta
     7621 gataaacaag agaagaagat agaaagcttc gctaacttcg atgcaagtga aaggtggtaa
     7681 gtgcatgatc atcaatgaca atggcagaga gtatgataca gaaaagattg aagagtattc
     7741 atcttatact cagggattaa ttaaacgttt gatatacgtt cgctatgtag gtattaggga
     7801 tctgttatca gataactgtt gtagtaaata caaagtgaat caagtaagag aagcgttgaa
     7861 taaagataat aacgtcgaaa gaataaaaaa tgtttttgga tatagtattg aagagattaa
     7921 ttattacatt gacttcgctg aagctttcat tccgatggtg agataacccc cccttaaaat
     7981 aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct gtcaggaaaa gagatttttt
     8041 cgaactttat catgaaagga gggctaaaat gtttaaaaac gaattgtctc aaaatcgcta
     8101 cagagaaaaa ttacgccgct ctttaataag ccaattggaa agtcagaaaa caaatattga
     8161 gccattctta gataatgttg atcgttatat cagtttatgg gaaacggcga tatcactgga
     8221 agaagatata tccgagaacg gcattagact ggagaatggt aaaaagaatg aatcagtagc
     8281 gttgcttgtt tctgtcaaca aacaaatggg attgatgttg gataaacttg ccattactcc
     8341 tgaattggta ggtgaagcaa atgaatcaat tcctgagtta taagcatatt gaaaattggt
     8401 tcaaagctat agaagaaggc actatcaagg tatgcaaaga gcaattattg ctaaaaaatt
     8461 atctagaaga aagagtcttt actagagaag atatttactt cgataagcag atggtagagg
     8521 attcaatcaa tataccagca caatactttc cattcgaatt aattccgtgg gaaaaatttc
     8581 tacaatgttt tatttatggt gttcgatgga aaaaagataa aacactagtg ttcaatagat
     8641 atctttcatt aatgggacgt ggtaatggta aaactggttt tgcttcttgg aacaacttct
     8701 ttctactaac cgctaaacac ggtattaaaa attatgatat tgatatctat gccaataatg
     8761 aaagccaagc aaagactagt tttgatgatg tatttaaagt aattaaagat catcctgatt
     8821 tagataaaaa agtatttaaa gctacgaagg aagttattca aaatatcgct acaaacagca
     8881 aacttcgtta taacacggca aatgctagaa caaaagatgg gaagcgacca ggtgcaaacc
     8941 gctttgatga aattcacgaa aatgaagatt attcaatgat aaatgtggct acttctggtg
     9001 gtggtaaaaa tcgagattat agagaatttt atgatacaac taatggtcat gttcgtggtg
     9061 gtccgcttga tgacattata gaagaatcaa aaatgattct ttctggagaa cttggaattg
     9121 acaaggatgg agcagaattt tctagtttgt ttccatttat ttgtcgcttg gataacgata
     9181 atgaagttga tgatcccgac atgtgggaaa aagcttgtcc aactattaat tacaatgcaa
     9241 atttaaaacg gaaaatgttt caagaatact ctcaaatgca acgtaatgct ggtttaagac
     9301 ttacgttcat gaccaaacga atgaacagac ctatggaaga tacacgattt gctgttgctt
     9361 catatgatga tgttctgcat acgaaagaaa aagaatttcc tgaaaaaatg gatgaagtga
     9421 taggaacagt cgattttgct gatagacgag attttgccag cgttgggttg ctaggaaaat
     9481 acgataaaga tgtttatttt acacaacata cttttatcca cgaatcagcc cttcgattac
     9541 aaaacatcaa acgagaggtt atagatattt ctatagatca aggaaaatca cagatcgttc
     9601 atggaaaaaa tatagaagct gattatattg taggttggtt tcttgaaatg agtaataaat
     9661 attatattaa aaaaatcgct atggatatgt accgtgcaaa aatattgaag cccgctttag
     9721 aagaagcagg ttttactgtg gaaattgttc gaagcggatc tgttacacat ggtatgttaa
     9781 aagatctggt tgatgacctt tttattaatc aacgtttatt ttttggtgac gatgcgatta
     9841 tgcgttggta ttgcatgaat gtatatgaag agcatatttc taatggaaat atacgctatg
     9901 aaaaaataga acctgaaact agaaaaacgg atggcttttt ttcattcctt catggtttga
     9961 attttttaga tgatatttat gattctgctc ctgtaacagt cacaaatagc tcagtagaaa
    10021 atacaggaac tggatttact cctctagtat tctaacttga aaggaggtga gaaagtgggg
    10081 atttttcaaa aggcggtagg atacttcaca aaaaaagcaa cggttacttt agaagaatac
    10141 ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg cgattgaaac ttgtattgat
    10201 ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat atgaagatgg aaaaaataaa
    10261 aagaatgatc tttactatag gctgaatgta gctcctaata agaaaaataa tgcaacagaa
    10321 tttagaaaaa aactgatcag gagattaata ttctacaatg aagtattgat cgtttctccg
    10381 tctaataatt ctagcgaaat atttattgcg gatagttggg atgtcacaga atatgcattg
    10441 aaagatgatg tgttttctca agtgcaaatt aacaacatag tccttgatag agaatttcta
    10501 gaaagtgatg ttatctatat aaaatacgca gatcaacaaa ttaggcaact agtcgatgcg
    10561 tattatcaag cgtatgggaa actcatttct agtgccatga atgtttacaa gcgctctaac
    10621 gctcgtagat acgtactgaa agggaattta ttccgaccgc aagacaatac aacacaagat
    10681 caaatcaata aaatgatgac atcacaattt aaggctttta tggaagctga taatgcaggt
    10741 gcggtatttc aattacaaaa tgagtacaca ttagaagatt tcagcggaaa cttccaaagc
    10801 aattcaagag atataaaaaa cttaatagac gacatctttg agatgacagc agcagcgttt
    10861 cacgttccga aaaacctact aaagggagac atgagtgggt tatcggatca agtggacgct
    10921 tttttaatgt tcgaaatcat accgattgct gaacttattc aggatgcgtt taacgctagt
    10981 ctctatgaag tagaagaata cttgtcaggg aattttgtac gtgtggatac aactatgatc
    11041 aagattacta gcttcaaaga tttggttgac gctattgatg taggcattag aaatggagta
    11101 tttacaatca acgaaggaag agaacgcgtt ggaaatgatc gctctgataa ggcgatggca
    11161 gatgaaatat ttataactaa aaacaatcaa caagtatcga aaggaggtga ggcgaatgac
    11221 gacaatgaaa acatttctag cagtaaagaa tgaaggcgca gtaccgcaaa tttttattca
    11281 gggatttatt ggttctagtt ggttctttga agggaatact gacaagggaa tcaaaaatat
    11341 tttggatagt ctaggtgatc aagaagaaat tgaagtagta attaattcaa acggtggaga
    11401 cgtatttcaa gggattgcta ttgggaactt acttaagtca aataaagcaa aagttaacgt
    11461 tgtgattaac ggcttagccg ctagtgctgc ttcaattatc gcaatggctg gcgatactat
    11521 aaaaatctac aacaatgcac aattgatgat tcaccgcgct tccacatacg gagaaggaaa
    11581 tgtcgatgac ttccgtacga ttgctgacca actggaatca attgataaat cggtaaaggc
    11641 ttcatataaa acacgattca atggcacaga tgaagcattg caagaacttc ttgaaaaaga
    11701 atcgtttatg gatgcagaaa cagctttgag ttatggattg gtcgatgaaa ttatcgatgc
    11761 agaaaatagc tcaggtactg aagctaaaaa agaacaaagc gttgaagaaa ttttgaatga
    11821 cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca gcagcattaa ataaaacatt
    11881 tggacaagga gatgtaaaat aatgacagtt aaaaatttaa aaggtgtaac agctgcaagc
    11941 gaccaattga tgaaagcttt taaagatggt aacgaagaat cttttagcgc agctatggta
    12001 agcttatcta aggaaattca ggataaaatt ttagaagaag caacagcaaa aaatcaagat
    12061 caattagtat taatgaaccg tggtcagcgt gtattaacta cacaagaaac aaaattctat
    12121 aacgaagtag tgaaaaacga aggttttgca ggggtcgaag aattagtgcc agctactgta
    12181 tttgaacgtg tatttgaaga tttagaacaa tctcatccac tattgcaaaa aattactttt
    12241 gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg gagtcaatcc agcatggtgg
    12301 ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg gctttgatgt aattaacatg
    12361 aagcagttca agctatcagg ttatattcct gtatgtaagg caatgcttga tttaggtcca
    12421 gtatggttag atcgttatgt ccgtactgtt ttagtagaat cattgagaat tgcattagaa
    12481 caagcaattg ttgatggtac tggtaaagat atgccagtcg gaatgatgcg tgacatgagc
    12541 aaacaaacta gcggagaata tgctgaaaaa aaagcagaac ctattacagc tttagatgct
    12601 gtaactatgg gcggtttgat ggcgcgacta tcaaaattca atatcgaagg tgtgaatgat
    12661 ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa ttgtgaatcc aactgattac
    12721 tggtctaaag tattcccagc taagactgta ctaactgcta atggagaata cgtacaagta
    12781 ttgccagtac cagtttcaga tttgcagtca acggctgtgc cagaaggaaa agcagttatt
    12841 ggggtagcct cagattactt catgggtgta ggatctacac taaaaattga agcttcagat
    12901 gaataccatt ttgttgaaga cgaacgcatt tatctagcta aacaatatgc aaacggtcaa
    12961 cctaaacgta acgatagttt cattgtatta gatattagcg ctttgggaac tactactaca
    13021 actacaaaac caacaaccac aacaactaca acacaagcgt aggtgatcag aatgaagtat
    13081 attctttgtc agccggcaat caatcggttt aaatgggagc ttgaagtttg tttaactaat
    13141 ctgaagaaac taggaatcaa agatatcgta ttgcttttca gcagacacga tgatcagatt
    13201 cctatttttt ttgagaagga atatggtgtt gaagttcatg tgtacgatga tctgcgggac
    13261 gacaaagagt atattccttc gattaaacca tatttatggt ggaaatattt agaagaagat
    13321 cattcgcgtg aggacgaccg atatttctat atcgattcga atgtcatttt caataaaaga
    13381 attaatttgc gcaaattgcc ttctaaagat gatgtttggt attgtagcga ctgctgtagt
    13441 tatctaagtc ttgattatat tagaagctgt gaaaacggag aaaatattct aaaagatatg
    13501 gcaaacattg taaatgttac agtagaatct ttggaaacta taaacactaa ttcaggaggc
    13561 gcacagtggg ttattaaccg tcctaaagcg aattattgga aaaaggttta tctggattct
    13621 aatcggctat atcgctacct tagagggcaa aaaacaaata tacaaatctg gacagccgag
    13681 atgtgggcac agctttggaa catgatgtat ttcaatattg gtcctaaagt tcacgaggaa
    13741 ttagactttt gttttgctac tgatccaata gaaaaagtta aagaagtaaa aatcttacac
    13801 aatgctggag taacaacaaa cgatgaagat ttatttttca aagggagata cgtgacttct
    13861 acgccttttg atgaagattt atcatttgta aacaagaaaa aatgctctta cgcatatgtt
    13921 aaagcaatta aggcggtggt tagatgacgc ctgaacaagt gactgaagaa ttgctaatag
    13981 ctgtgaagga taatatttac gttacctgga acgaagaaga tgagtcaatt aaaaagatga
    14041 tagctaaaaa tgctgtttat cttcaaagta aagtgagtac aacactttct ttttctcctg
    14101 aaagcttaga atacggattg ctaatcgaaa gatgtagata cgactggaat cgtgctttag
    14161 atgagtttga acaaaatttc gctagtgagt tattaggttt cattcaacat tatgcgctac
    14221 aagaatatat tgcaggtgat gtgaatggcg aataatcgta gactcgaaga aacattcaac
    14281 gatggttggt taaagatttt gacgcaaact accaaaagaa atgaactagg aaaaaagatt
    14341 ggtgtagaag atacagaaat cacttcttta aaatttagaa atctttccat gagagatagt
    14401 gatataacag ctatggatgc gatgggatcg aaattaacta agaaagtaaa gactccattt
    14461 catccaatcg ccaagaaatt taataaagat caatatttta tcgtaatcga tagtatgcgt
    14521 tacaacgtta tctatgccga ttacgataat ttttatatct atttttatct tgaaagtgtg
    14581 ggtgaatatg gtgattgata attctaaaga aaaagaacgt ttaaataagc aaatttctgc
    14641 tatcaaaact tccttagaag agcattttgg cctcaaactc tttcaagact ccgtaggcga
    14701 ggatgagcta cctgatgatt ttaattactt cattctcgaa acaggagaaa tagaaatgat
    14761 cactgagcca aaatatagcg tgggtcaaaa tctatatcta actttctatt cagaaaatag
    14821 agaagattta acaggagact cactagatat tatttcattg attcaaaatc gttcgattcg
    14881 ttttcagaga atggatccca atcatttaaa actagagaac caagatcgct atatcgatca
    14941 attggtattt acgtttagac gattattgaa gagtgattgt catggctaaa aatagttggg
    15001 agctaaaaat aaatggacat gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga
    15061 gcgaacgact gattaacgaa gcattgaaat caaaaggttc ggctattgca gtggatagga
    15121 ttacagaaaa aattcctgtt tctgaagcag atttaagaag agggcaccaa cacgcaaaaa
    15181 atagtcgtcc acttaagact caatacatta atttgggttt catcattaga cctacaagaa
    15241 aatttgagta tttaaaatat cctgatttgg ggataggtac ttctaaaaga aatcagccgg
    15301 acgaatttat gagaagagga ttaggtcttg cacttgatcc aattacagaa cttctgattc
    15361 gtcaattcga taaattaaat aaataggggg aacaacaatg gctaaaacaa aaactgtagt
    15421 aacaacgttc gataacataa gtatcaaacg aattgctttt aattttaaga acgcaggaaa
    15481 tgcaatcgta acgaattgta atggacaatt agatggcgaa acagaaatgc aaacggtggt
    15541 taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa ccaatcaata tgacggtaac
    15601 aattactgca catgtaccga tggaagttta tcgacgtttc aatgggttga aacaagatgg
    15661 acgtattaaa ccaggcattt actcttacgg tcctgattcc gtaggcgaag atttctcact
    15721 tgctgcagag atcgtggatg acttcgaaga aaatagaaag ttagttggta tgttagcatg
    15781 cacttcgaat acaggattaa cattctctat tgaaaatggt gcggatgaag tagctgcgtt
    15841 agaactagaa acaaaagtta tgcaagatga atttggtaaa ttttatcatg aagcaattgt
    15901 tgcagaactt gaagaagact taacagatca atggatgacg aatctatctg ctgatgtgat
    15961 taaaaaaact tcagttgtga caactacggc cactcaatca cagtaaaaaa aacggaggta
    16021 gcgaaatgaa cgaagattac tcaaaaattg aactaaacga tggaacaatt ttgaatttag
    16081 aacctaaact gaatatcaag aaattattga tgatcaatag agattttaac acagacgagt
    16141 ttgcaaaaat gtcgatggga aaaggctcta tggatattac agttattcaa ggtgcaaaag
    16201 ccgtatatgt agcttatcgt caagcgaaca tggtcgatta catttcattc gatgaattta
    16261 tagataaatg ggattttgat atggaggttg cagtagctgt atacagtact atgatgttca
    16321 aacaagcacg tgatgcctat caaaaagaat tcgaaaaagc aaataaggaa aaaaagcttc
    16381 aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc gatgtctatt cgatgttgac
    16441 cgacgttttt tctatgcctt cagatttggt tttaagcgat atctgtttag atgacatttt
    16501 gcaaatggct tacaacaaga gcgcttatga aggatggaag aactatgcaa taaatcaatc
    16561 ccagaaaaac taaaggaaag gaggtaaaaa atggctaaaa agagaacgga agcagaagta
    16621 actttcatag ctaacgatga cggattgaaa tctacgttaa aagaaatcag tgctgaatta
    16681 actaaaaata gagcagaatt aaaactagaa caagctcaat tacaacagac tggttctgaa
    16741 tcagacaagt taggaagtaa attatcttct ttagaaaagc agtatgaatt acaaagtcaa
    16801 aaagttgaag taactagtca acgtttagcc aatgccaaaa aatattatgg agaaaattcc
    16861 accgaagttc agaaacttga aagagaactg attaatcaac aaacagctca gcaaaggtta
    16921 tctaatgata tagataaaac gagtaaggca ttggctcaat ctaaaggtga atttaaaacg
    16981 tatgagtcaa ctatgaaaga cttagataat gagcaacaac atctaaagtc aagcgcatca
    17041 ttagtagaat ccgaatataa aaaatggcaa gcaactgctg gccagtctgc tacagaatct
    17101 gaaaaattag caaaagcaca agaatatgtc ggcaaacaga gtgatattgc tgaacaaaaa
    17161 atcgaagtgc taaaaaaaca attagatgcc acacaaaaag aatttggaga aacatctacc
    17221 gaagctttag aaatgaaaac taagcttaat gatgcggaaa gagaattcga agaattaagt
    17281 aatgctgcta aaaacgttga tacctccaca gtagatgata tcggcaaaaa acttgatatg
    17341 ggtaatttaa tggaagctac tgatcacttg tcagtgatcg gagataagct tattgatgta
    17401 ggtagtaagt ctattgaagc agctggaaaa gcacaagcta tgcaggccca atttaaacaa
    17461 gtctttggct ctttagaagg ggaagcacag gacgccgttg agggaatggc tgaagaattt
    17521 ggaatgttac caaatacgat caagcctgtt tttatacaat atacgtcaat gtttaaagga
    17581 cttggatatg ataccaaaga agctatggag ttagctggtg atagtactca gttagcagca
    17641 gatgcagcag ctttttacga taagtctatg gatgatgcta gtgaatctct taattcattt
    17701 ataaaaggga actacgaagg tggggagcaa ataggtttat ttgctaatga tactcaaatg
    17761 gcagcttatg ctgttaagca taatttgata ccagcgactg aaggagcaaa aaaagccagt
    17821 gaagagtcat tgttagctgt tgaaaaagca caatctaagt atgctgatgc tattaagaaa
    17881 catggtgaag gatctttaga agcaagagag gctgctttaa aacttaaaga tgcgcaagat
    17941 aaaataaatg aagaattagg cccacaaacg caaaaatggt ctgatttaga tgaggctacc
    18001 aagcaagcag ttcgagttca atatgctgaa gatatgcaaa aattagccgg tgctacaggt
    18061 caggctagta gggagtctga tggtttagaa aaccaaatga caagagcaaa gcaagcgtta
    18121 gaagattttt atgcctcatt gggtgaagat atactgcctg tgtttatcaa agggttgcaa
    18181 gcaggagcga aagctttgca aggattagcc gaatggtgga gtaaacttga tgggccaatg
    18241 aaaaatttca ttttagctct tggaggaatt ctagcattat taagcacatt agctcctgtt
    18301 ataaccgcag ttgttacgat agttggcaca tttggttcta cagttttgct gccaataata
    18361 ggaattatag cgggcgttgc agctgtaata gcgattgtaa ttacagcgtt tcaaaactgg
    18421 ggcgcaatca ctgactggtt tagtgattta tggaaaaaat ttaccgattg gttaggtgat
    18481 acctgggaaa gtataaaaga aagtgcctca tcagtttggg atggagtcaa agaaacctgg
    18541 tctggatttg tagattgggt tcaagaaatt tggcaaggag tttctgattg gtttggagag
    18601 ttatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca agagacttgg
    18661 caagcattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt
    18721 atttgggcag acattgtagg aattgttcaa ataccatgga cattaataac gtcattgatt
    18781 caagctggta ttaatattat cgtgggtatc tttgatgtag ctggacagtt attaggcgca
    18841 gcttggcaag ctgtttggac acctatttct gatttcctta aaaatacttg ggatactatg
    18901 acacaatgga taagcatcgc ttggaatgga attgtaacta cattccatac tatatttgat
    18961 ccagtagtgg catggtggaa tggtatatgg acatctatta gtactacggc ttcaaatatt
    19021 tggaattcaa ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact
    19081 agcttggtac aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg
    19141 ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggaa tagtgtgact
    19201 agtagtataa gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt
    19261 atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa atactgcttt gaatctttgg
    19321 aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc
    19381 ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt
    19441 gctaacatat gggaaggcat caaaaagact gttagcgctc caattgattg gattagagac
    19501 aaaatcagtg gcatttttga taatttgaat atttctatac cacatattcc gttaccggaa
    19561 tttatcatgg aaggcagctt taacccgcta aaaggtcaaa tcccccattt gcgtgtaaaa
    19621 tggcatgcta aaggaggtat ctttactaaa ccaactttac taggtggaat gaacggtgtc
    19681 ggtgaagcag gacctgaagc agttttacct ttgaaaagat ctgttttgca agaaattggt
    19741 gatcgtatct tgagtagcac atcagtttca tctagggcac aaacgattca acctgtgaat
    19801 aactacgaat tcaatttcac aattgatggt aacgcagatg aggttactat gaagcaaaca
    19861 actcaacaaa tcattgatag cattacaaaa attcaaaatg ataatgcttc ggcatggcgt
    19921 taaacaggag agtatttctc ctgttttttt agtattaaaa aggatgtgaa aaaatgactg
    19981 attgtataca ttctataatc gatggatttc ctgattattt gcataaattg gctttagctg
    20041 aaagaccaac cataccttct ccaaaaagac agagagttga aacttctgtt ttaggtaggt
    20101 taggtggctt agtacaagat tactcgtttg aagatatgtc gtttacattg cactataact
    20161 atttagagga tgtggaagac catcaagcgt tcaagcaatc gttttatatc atgcgtcatt
    20221 ggttaaacta tgcaaagaaa ttagaattct ctgacgatcc caacgtctat tacgttatcc
    20281 agactatcga tattggggat gcagaaaacg atattgttga atggggagag ttcgatgtaa
    20341 atattactgc gaaaccattc gcaagagttc aagaagatgt acctataacc gtagataaac
    20401 cacagtcatt taacttgctg aataatagtt tagaagaaag ttttccaaag attatcatca
    20461 ctccttcagc tacttcatgc cagttcatct taaatgatta tgtgtttagt ttcgaaggct
    20521 tagtagcagg aactgatgta gtcattgata gtgatttgat gctttgctac gaagagcaat
    20581 cggacggaga tattttagat cggtccaaca aaatgaagac catgcaatat ccaacgttgc
    20641 aagtggatat taatcatttt aattgtactg gtttgagcaa aatacaaatt tatcgtaacg
    20701 ggttaaggta ggtgaaatag atgatcgata atttaataac tatttacgat aaaaatgacg
    20761 cgaataattt agctgaacat ttatatgata cgcaaggttt aggcgctttg tcagactggt
    20821 taacagctac tgttagcaat aaactaaacg gagccgagat atttcagggt acttattcaa
    20881 taaacggaac taatgcagat ttgattgtag aaggacgtat tattcagtgt tatgtagatg
    20941 aaaatcgagc aaaacagcgt ctacggattt attatgcaaa aacttctgta ataggcaata
    21001 cgatagaagt aaaagctgaa cctattttca atgatataag aaaatcggtg ttgaataaat
    21061 atgacagcgg aacagaaaag atcactgcta ctcaggcatg gcaaaacgca aaagttttag
    21121 cgaaaccagc tatcccttcg cagttttctt tctcgtcatt agtagatacg cttgctaatg
    21181 tgaagataga aaaggcgaat tttttagaat tctttggtgg aaaagaggga tctattctag
    21241 atcgatttca tggtgagttt ctaaaagata ataacacatt acgtcatgaa aaaagtctag
    21301 gtacggatca taaaatcaaa gcgatttata ctaaaaactt aactggtctt gacttagaga
    21361 ttgatgctca aagtgtttta gttggagttt atccattcat tagcagctct tcagaaggag
    21421 aagacgagat cactctacca gaagaagtta ttttcacgga ttacgtggat gattatcctg
    21481 ctggatatgt ttcttttgtt gattttaaag acaaagcgac tgatgtagcc acattaaggg
    21541 aagctgctaa agactggttg aaaacaaaca tagataaaca aaaaccacaa gtgagtggtt
    21601 cgattgaatt agtaccattg aggcatcaaa gaggctatga aaaatttgtt gatctagaaa
    21661 aagtttcgat gggtgacgga gtagatgtgt atcatccaca gttaaaagtg aatatgtcag
    21721 cgagaattgt ggaatatacg tttaatgttc taaccaattc atacgataaa ttagttgtag
    21781 gaaacgtcaa aacaaacttc ttagaaaaca cagagaataa tgtcagcaat ttgattaatg
    21841 atgccattga tcaattgaaa aatggtggcg aaatcagtga tttaatcaat gatattgtag
    21901 atcatcaaac tgatatgatt actggtcaaa atggtggtta cgttttatta gatcctaaag
    21961 aagcccctag tcgtattttg attatggaca caccagataa gaatactgca cggaacgttt
    22021 tgcaaatcaa caacgctggt attggtttct ctaaaactgg cattaatgga acgtatgaca
    22081 ccgcatggac gttagatggc ggattcaatg cctcgtttat tacagctggt gagatagtag
    22141 ggattactat tagaggtact acattaatta gtgatggcac tgattataga acaagtatag
    22201 ctaatggcaa aatgacttgg tactcaaaaa aagttaacaa agatattatg gagctagaag
    22261 cacgtgatta tgtaagtgct gatgccggca ttgtatcata caccatgaaa actggtggtg
    22321 gtttcatgat tagaaatcca cagggtaact tggtttttag tacgtgggat aatggtaata
    22381 acagaccgtt tttatctttt ggtgcgccaa atttcagata cagtaatgct agttatataa
    22441 atgatggtga cggtaattct ttaggtattg atggtagtgc aggtaattca tgggaattta
    22501 aagtagctgg taggactatg aaatttacta gtgacggtat gctaacgttg ccaggttgtt
    22561 tttttggttc atgggaagat gggaaacttg ctagatttga acaatcaacg gtacaagtat
    22621 ataaagattt tactgttaga ggtactaaaa actcaaccgt accaacagaa cattatggac
    22681 aacgactatt gaacgcttat gaaactccag aatattattt cgctgattat ggggaagccg
    22741 ttacaggtga tgatggtaaa gttcgtgttg atattgaccc catgtttgct gagacagtaa
    22801 atctaagtcg gtatatgaca catgtgacac ctacagaact agttttgtgt gctgttactc
    22861 atgaagatgt tgaccatttc atcattgaaa ctagtaagcc aaacgtattg gttagatgga
    22921 atttagtggc acatcgacta gggtatgaag atgttagatt aaaagaggat acagcatatg
    22981 atagcacagt gcttgaccaa aaacgttttt aaaacgaaga caaggaggta tataaatggc
    23041 tagcagttta tataatttgg ctttagattt cagcaaagaa ttaaactaca ccaaagctat
    23101 tatggctcgt caaggtgata aagggatcac ggtgactgtt aaaccgtttt taaatggctt
    23161 gcagatggat acgagcggcg gaacatttac tttaaaagga actacgccat ctaaccgtta
    23221 cgtagatagt gtcgctacta gtgtaactag tgaagaagtc acattttctc ttgatggcac
    23281 atttatgagt gaagcagggt attataaaca ctgctatgta gaatatagaa aagacgatca
    23341 aattttaacg acgcaagata tcattttttt ctcactagga gtgtctgaca tttcgcaagg
    23401 ccaagccgat gaatatgttt cgcaattaga agaattgatt cgaaagtata acgaaacttt
    23461 tgatgctttt ctggctgaaa ttaaaggtag aatggatagc ttaaataaac agattactga
    23521 tttaactggt caagctaaaa cactacaaga caagttagat gctctgaaag aagaaatttc
    23581 taaattaggt aacttacatg tgatgtactc caacagcatc gacttcgggg gctatgatta
    23641 tagtgggaat ccgaatttaa tgtccaaact aaaatcgagc gattttaacg ttggttacca
    23701 cgggtcacta actaatgtta acggaatgtt acattttatg tctgatggta caggggacat
    23761 tgatatgttt acgcgtatta atacacctca gcttgctagt ggcaaaacat atactctgag
    23821 tgcaaaagtt cgatttgatg aaggaactac aggagctatt aataaattac gtttggtata
    23881 tcgtacatca ccaggagaaa atatattatt ggaagcaaat aatacaacta ttacaataga
    23941 tgatgtaggg aaagaaataa caatcaaagg cacagctaac gttaattatc aaatcacaaa
    24001 tttagatcgt ttttatatta gtgttagttt ttctaacagg gataaaataa atggtggatt
    24061 taaattgtac gacatcaaaa tcgaagaagg ctcaacagcc actccgtatc agccaaactt
    24121 actagatgac ccttactggg taggtaaaac atcgttgggt aagaatattg caaataaagg
    24181 tacgacgttc ccaattaaat ctagcgcgta cgaaatatat aaaggtaaca cggaagaaga
    24241 gcttataata ggtcaaacgt atactatcac gcttaaagga acaaaacccg caagtcaaac
    24301 ctttgtagca tataattatt ggaacgttaa ttttggagat ctaaaaccag ttgagggatt
    24361 gacagacgta tggtctctaa cattcacacc aacgaaactt gaaccgggtt tgcctaaaga
    24421 ccttcgcatt tttcagtcgc ctaaagaaac ggccggcgca tgccgaattg actggctcaa
    24481 gatcgaaaaa ggcgacacac gaaccccgaa tattgagcaa tataaatacc gaggaatcgg
    24541 catgcgagac tcaaacaatc caaaagatta tgtatgggat ctagaaccta gctatgttga
    24601 gaataaccta gctacagata tcaaggttgc tgaaatcaca ggtaaagcaa acaattatac
    24661 cgatgggaaa gtatcggaga ttaattcgca gttgactgct tcaattaatg aagtagacac
    24721 cacagctaag gatgctcaaa caaaagcgaa tgctaatgcg actgctatag atgaattaga
    24781 caataagatc gatgaacgca ttaatgatac agctactacc acattaacag ttacaaacgg
    24841 gaataccgga tcagcaaagc tttatcgtga aggaaaaaca gtttctatat attttgtggc
    24901 tttaaacgga aaaagcagtg gtggaaatga ttcaacgata ctaacaattc cagaaggcta
    24961 tcggccacca attagttttg agcaactggt tggctc
//
LOCUS       DAZUKT010000042        23849 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000042 DAZUKT010000000
VERSION     DAZUKT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.042"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1025)
                     /locus_tag="ACG816_001630"
     CDS             complement(<1..1025)
                     /locus_tag="ACG816_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4920508.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLT"
     gene            1435..1734
                     /locus_tag="ACG816_001631"
     CDS             1435..1734
                     /locus_tag="ACG816_001631"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4920509.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            1807..3168
                     /locus_tag="ACG816_001632"
     CDS             1807..3168
                     /locus_tag="ACG816_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4920510.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            3190..3450
                     /locus_tag="ACG816_001633"
     CDS             3190..3450
                     /locus_tag="ACG816_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920511.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            3464..4216
                     /locus_tag="ACG816_001634"
     CDS             3464..4216
                     /locus_tag="ACG816_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4920512.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(4310..4915)
                     /locus_tag="ACG816_001635"
     CDS             complement(4310..4915)
                     /locus_tag="ACG816_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4920513.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4968..5942)
                     /locus_tag="ACG816_001636"
     CDS             complement(4968..5942)
                     /locus_tag="ACG816_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4920514.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(6320..7039)
                     /locus_tag="ACG816_001637"
     CDS             complement(6320..7039)
                     /locus_tag="ACG816_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4920515.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(7166..8437)
                     /gene="celB"
                     /locus_tag="ACG816_001638"
     CDS             complement(7166..8437)
                     /gene="celB"
                     /locus_tag="ACG816_001638"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4920516.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            8590..9987
                     /locus_tag="ACG816_001639"
     CDS             8590..9987
                     /locus_tag="ACG816_001639"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4920517.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            10068..10508
                     /locus_tag="ACG816_001640"
     CDS             10068..10508
                     /locus_tag="ACG816_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4920518.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            10656..10982
                     /locus_tag="ACG816_001641"
     CDS             10656..10982
                     /locus_tag="ACG816_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4920519.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10983..11732
                     /locus_tag="ACG816_001642"
     CDS             10983..11732
                     /locus_tag="ACG816_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4920520.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(11812..12267)
                     /locus_tag="ACG816_001643"
     CDS             complement(11812..12267)
                     /locus_tag="ACG816_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4920521.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(12694..12969)
                     /locus_tag="ACG816_001644"
     CDS             complement(12694..12969)
                     /locus_tag="ACG816_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920522.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            13263..14078
                     /gene="thiD"
                     /locus_tag="ACG816_001645"
     CDS             13263..14078
                     /gene="thiD"
                     /locus_tag="ACG816_001645"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4920523.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(14407..15858)
                     /gene="cls"
                     /locus_tag="ACG816_001646"
     CDS             complement(14407..15858)
                     /gene="cls"
                     /locus_tag="ACG816_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4920524.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            16199..18865
                     /locus_tag="ACG816_001647"
     CDS             16199..18865
                     /locus_tag="ACG816_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4920525.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            19229..19981
                     /locus_tag="ACG816_001648"
     CDS             19229..19981
                     /locus_tag="ACG816_001648"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4920526.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(20003..21157)
                     /locus_tag="ACG816_001649"
     CDS             complement(20003..21157)
                     /locus_tag="ACG816_001649"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4920527.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            21342..23783
                     /locus_tag="ACG816_001650"
     CDS             21342..23783
                     /locus_tag="ACG816_001650"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4920528.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7641 a         4189 c         4945 g         7074 t
ORIGIN      
        1 tttgtcagag gtgtattttg cgactgatag tttccagatt ggttcacttt ccaacttaat
       61 ccagcgtatt ttgccaattt tgtctgatct tcaaatcttt cgatttgacc aatttcagcg
      121 attaaaccgg cagcatagac tttaccaacg cctggaatac tggttaagca ttgatattct
      181 ggaatgacaa caattacttg ttcgatggct ttatctaatt ctttgatatt tttttcaagt
      241 gctcgtattt ctcgtacgag aacacttaaa acaacattaa ttgattcttg tgcaagagca
      301 cctaggcggt aactcatggt aattgcacgt tgaattgctt tagcgatttt ttctggttgc
      361 ttgaaacggc cttttccttt ttcttgaagt aaatcacaga aagcttctaa aggaagctca
      421 gcaagttcat ccaatgtgaa atcttccgtc atgagtgaaa tgagggttgc actaaaaaga
      481 ctcgtcgatt cgtctcgcat ttctcgagca agcgtattac atttgtacgt tagattttca
      541 agaaaatgtt gctttgtacg aatcaattgc ttaataagtt gatagcgagt acgtgttaaa
      601 cgctgtaagg ccatgtattt ctcttctttg ataggggaag ttgtgaatcg ttgaatacgc
      661 aagaaatcag cgattcttaa tgcatcgttt cgatcagttt tattctcatc aaacatgcga
      721 ctaaattgtt tcacccgaaa aggattttca atcgttacaa gtgtgttgag cttctttagt
      781 ttctcatctt catgaaaaaa catggaagga tggaagctgt acatggacgt tgactccatg
      841 ccaatcacaa tttgatcaaa gtggtagctg ttattgaatt caagaattgt ttctcgtatg
      901 aatgaagcac cttcgatgtc attggcaaca gagatttcag ataagataga caattgattg
      961 tcgtctgtta aaaaacaaac atctagtttt tcagagctaa cgtcaatacc gacaaataat
     1021 ttcatggaag aaggacctcc tttcgatata aattttggaa aatgcttcga tactgtggga
     1081 tttcccagaa actcactgta tagccacaac ctcgcctaat agaatacggt gataaaagag
     1141 ctagaagctg ccttccaaac tactatttga aagtctagtc cattatcgaa acagctaatg
     1201 agttggtagt gataacagtg gttcgggtaa cagactaaac agggtagaca aacgctacag
     1261 gaggaaatag caaattcccg ttggatcctt tccatacatc cattgtactc tgagaaatca
     1321 cacaatatcg aaacaaattc cgttaggaat gtgttattca ataaatttta taatctagaa
     1381 acattttgaa taaaaactat tagacaaatg atattttacg aggaggaaga aaaaatgaaa
     1441 aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat cgctagtaag
     1501 atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt tgattacaaa
     1561 tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca acctgatgag
     1621 gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat tccattttta
     1681 actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca gtaaaggagg
     1741 attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa gaaagaaggt
     1801 ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg atcagccatt
     1861 tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt aaaaaaagca
     1921 ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt cattggctat
     1981 atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg aatcagtttg
     2041 cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg gagttatgct
     2101 tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt taattggacg
     2161 aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc ttatttagtt
     2221 tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca agttatattg
     2281 gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg gattccatta
     2341 gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt caatatgatc
     2401 atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa gaagaaaatt
     2461 gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct tggctttgct
     2521 gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac agcattaagt
     2581 ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc agacgctatg
     2641 tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt ggactggccg
     2701 atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc tgtgtttatt
     2761 ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat tatcaactat
     2821 tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat gctgatcttg
     2881 ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc aatactcagt
     2941 gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt gatcacatgg
     3001 tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa taggaatatt
     3061 ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta taaatatatg
     3121 ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca ttgaaatggt
     3181 gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc tctttgtggt
     3241 acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa tctattggtc
     3301 atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag agagtacgat
     3361 gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac caaagaaata
     3421 ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag atggacgtat
     3481 gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt tcgaaaggaa
     3541 aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc aagggaaaga
     3601 ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga tttctcctgc
     3661 acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg gtcatttaac
     3721 tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca aagctgtcct
     3781 acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta tgcctaattt
     3841 gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc ctaattgctc
     3901 tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg aattaccgca
     3961 tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat caggaatcga
     4021 agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag aaattgccta
     4081 caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa aaggtgagaa
     4141 aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat ctgttattgc
     4201 agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa atctgatcca
     4261 gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt tatttagtta
     4321 agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac tgatgacctg
     4381 ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg gccaattctt
     4441 taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag acggttttat
     4501 cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa aacaaccctt
     4561 tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg ccatttgaat
     4621 agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt tctttgattt
     4681 tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga ttcaatccat
     4741 tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa gaaagcaatg
     4801 tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg ccacctgtgc
     4861 catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag gtcattttgt
     4921 tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta ttcacgtttt
     4981 ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc acgacgttct
     5041 tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc atcttcaagg
     5101 aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag gaagtactca
     5161 gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt attgattact
     5221 tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg atggatattt
     5281 ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat gcttgcacca
     5341 tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt ttctttgaat
     5401 cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg gacaaagaca
     5461 ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca aggaatgcca
     5521 gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt atcgaaatct
     5581 tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt aacgtcatat
     5641 ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg gaaagaagca
     5701 cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc aaagaaagtc
     5761 atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt ttcgatgtca
     5821 tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa aaagcggtag
     5881 atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat ttgtgtatcc
     5941 atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga catagtatag
     6001 agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag gaatcaagaa
     6061 tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct gtatcgattt
     6121 caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca taagtctttt
     6181 tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga atgttgattt
     6241 cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt ggtaaacggc
     6301 tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag ttccgaacat
     6361 caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga tagacgactt
     6421 gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga tcatattcat
     6481 cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc aattcttgat
     6541 gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc acaagatgga
     6601 ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt aaccggtgaa
     6661 tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat tgattaggga
     6721 attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt ttcatttgga
     6781 gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat ggatgattca
     6841 agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct aagatattga
     6901 tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg acttgattag
     6961 gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa atatttgcga
     7021 tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag attatgcttc
     7081 cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc atgacaagtt
     7141 tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt ttatctagta
     7201 ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact tgcagcatac
     7261 caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca gtccatggaa
     7321 cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa ttgaacgtcg
     7381 ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat acgatcggta
     7441 gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct aatcgtccaa
     7501 catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca gctaaagtca
     7561 ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg atgatgtgtg
     7621 gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta ttccaaatcg
     7681 ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat aagaattgga
     7741 caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt aatggttttt
     7801 ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc atcacagcat
     7861 tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca aatgattttg
     7921 ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc gtgattctgc
     7981 agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct tttgcgccaa
     8041 gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc attgcaaaag
     8101 gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct tcaacgtcat
     8161 aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg atgctcattg
     8221 ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg tcactgaaaa
     8281 aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg aaggaaagca
     8341 tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta gctaagggca
     8401 tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc aaatttattt
     8461 gcaatcgatt acatacaaag tataacgcca aaaattttaa atgtctagtc attttcataa
     8521 aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa aaagagagga
     8581 agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc tgctgctagc
     8641 ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat ttgggatcat
     8701 tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa aacttcgcaa
     8761 gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca taacacattt
     8821 cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat caatcaaaaa
     8881 gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat tgaacctttt
     8941 gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg atggttatca
     9001 agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact gtttggagat
     9061 ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg aggctacttg
     9121 tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt aggctatcat
     9181 gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca tgatggtgag
     9241 atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa cccagaagat
     9301 gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga ccctgcagtg
     9361 aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat aatgccagag
     9421 atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct aggaatcaat
     9481 tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga acatagcccc
     9541 atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa tccatatcgt
     9601 ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag agaaaactat
     9661 ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga agaacgattt
     9721 gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac ggatcatttg
     9781 aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca catgtggaca
     9841 tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt tatcgctgtt
     9901 gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt taaaacggtc
     9961 tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca aaatgtttgt
    10021 tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg gtagatactt
    10081 atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca tttcttgagg
    10141 caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc aatgaagtaa
    10201 caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa gaattggatg
    10261 aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa ggcctgacag
    10321 atatcgagac atatgttctt tacggttatc caaagacact gattgctaat ttccatgaat
    10381 cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg gtagaacgtg
    10441 cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac gtcatggttg
    10501 taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt ttgctttctg
    10561 ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg ctattctaat
    10621 tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat ggaatacgcg
    10681 gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat ggatatgaaa
    10741 tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag acaacacttt
    10801 actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct ggagaagtga
    10861 ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat cttttagaag
    10921 aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg gagggaaatt
    10981 aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct caaagtaccg
    11041 agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat catgaattga
    11101 tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca tttggttcaa
    11161 tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac gagacggaaa
    11221 caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa tacagagcgg
    11281 caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca agttttcttt
    11341 tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc ggtatagcat
    11401 tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat cactttttgc
    11461 accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa gaagagtatc
    11521 agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt tcacttttcc
    11581 cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct atattttttg
    11641 ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt cattcttatc
    11701 gtcaattcac tcaaagcgct tattactggt gaatgtattc agatttgtaa aaatattcta
    11761 tttatccggc gtagaaaaaa agactgtgac acgcttttgt cacagtcttt tttatatata
    11821 ataatttaaa aattcatcat ctaaatccgt agaattttct agattgctta cccatgtaaa
    11881 atcgttatag acagatttca gttgacttct gcgccattgt acagggataa aaagacgcat
    11941 gctatcttca ttgaatccga ctaataattt ctttccgtca gtaatgatgg gacgttgtaa
    12001 caaagtaaaa tgcgagacga tcagatcgac aagctctgag gtcgtacatt ggtcgatttg
    12061 gtctttcaaa tttttataat caatcgagcg tttagctaat agatcgtcaa atccgttttc
    12121 tgttaatgac aataaataaa atatctcttt ccttgttaaa ggctgtttag gtgtgaaaat
    12181 tcgttctctg taaggcattt gatggtcttt cagccagttt gccatcagat ggtaactatg
    12241 tgtgcgagaa gaatagatcg taagcataaa aaaactcctt aaatagtcca ttaatttgtt
    12301 agatctgttg agataaataa taggtaagga gctgtgcata agcattgatc aaaacaaccc
    12361 aaacatcatt agaaataact ttctaaagtc ttaaacttgc gacgagaagc aactagacta
    12421 atagaaaaaa tgtcttagcc atttggcttc ctgtttgttt ttttgcttat gctgcagctc
    12481 ctcgggaaag ggttctaaca atttgaagaa ggttgatgat tgatctagtt taaattgtgt
    12541 gaaatttgaa atctggtgtt tttactagat gaatcaccta acaactatga ttataactta
    12601 tagaacttct gttttgcatt cggttttgtt atagttgtta taaagtttta ttttattaca
    12661 gaacattttt aattaataaa gaacagttag aagttaaaat tgctgattca catagagttt
    12721 atgacttctt ggaaattctt tttcaatcag ttttttcatc tgtttatgaa attctttttt
    12781 ttcagtaaac ataaaggttt gggtatcgta accttcttca tgtaggaaat agattattcc
    12841 tttttctgtg ttttgcgtat aagcttttat actcctgtct tttttgaaag aacagatcgt
    12901 atagccatct atttggtctg gtagtcgttt gattttcttc aaaatgactt ttttatcctc
    12961 aagtttcatt tttttcacct gactttattt ttttcatatg gaaatcatag cgcctttggt
    13021 tatatgaaaa caagaaggat actgaaatat atataaatca aatagtggtt atttattatt
    13081 agaatagcga atacaaataa acgaccacta ttttctgatt tattgcaaat gttttttcaa
    13141 ataaatttga ttaccaagaa aaggtagtgg tatatttact gagggatact ttgaaaaaca
    13201 tatgatagaa acagtaacag tattatgaaa aatcttttta taaaaatgga ggaatcgatt
    13261 tgatggaaga tcagtatgta caagcaatga cgatcgcagg atcagatagc gatggaagcg
    13321 cagggatgca agcagatatg catacattct ttacaagaaa tgtttacggt gtttctgtca
    13381 tgaccgcatg tgttgcaggt aattcttacg gaatcggagc aagtgtgaca ttgccaacag
    13441 attttatcga caaagaattt gaactgattg cgaaggactt tcaaatacgt gcagctaaaa
    13501 caggtatgct agcagattca aagttgattg agacagttgt aaaaaattac caaaaatatg
    13561 attttggacc attagtcgtg gatcccgtaa tcgtaacgaa acatggaaat cttttactag
    13621 aagaatctgc tttgcaaagc ttgaaagaaa agcttgtccc tttggccgaa gtactgacac
    13681 ctaattttta tgaagcagaa aagctgactg gacggacatt agcagctgaa gaagattttg
    13741 tgcaagcagc aaaagaactg caagatatgg gtgcaaaaaa cgttatgatc aaaggacatc
    13801 atgctaaaaa tgcgcagcaa gaaacagtca atgactacgt attgttcgaa tcaggaaaat
    13861 cattttggtt aaatgcacca ttttatccta ccgaccggat caacggaact ggcgattcgc
    13921 tatctgcatg tattgcagct gagttagcga aaggaaaaac agttgaacaa gcgatccgat
    13981 tggcaaaaga ctatgtaaac cgtgcaattc gtgaagaaat aaatgtaggg cacaaatatg
    14041 gccccatcaa tcattgggca gctggtgaat tagagtaaaa gaaagaagtt gtgaaaaagt
    14101 tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg
    14161 gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggtcgtgagc
    14221 ctgccgtcca gctccgagtc acaaggaaca agttcaaaaa acagcttctc atgtttgttg
    14281 aatattgtga cttgtgaccg aggagttggc aggagaacac cgtttgttcg gaagaaggga
    14341 ctgtgacaag atttttgtca cagtcccttt aatcttagta tatttttctc tacttgaata
    14401 aaaaaattat aggattggag aaaataatcg agaaatttgt tgcttgaata atagccagtt
    14461 agacatttcc ttgactgttt cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa
    14521 ctgagtagca agttcttcta aaaactcagg atcgtaaatg acagcacttg tttcaaaatt
    14581 caatcggtag ctacgaatat cttgatttgc tgaacctacc atgcagattt catcatccat
    14641 tatcaatgtt tttgcatgga ggaagccgtt ctcatagaca aggatttgta cattttcctt
    14701 gatcagctgc cgagcataat attgtgttgc tcgataaata aaaggatgat ccggtttgtt
    14761 gggaatcatg attttcacat ctacaccaga ggcagttgcg atttttaaag cagcaatgac
    14821 actttcatca ggaacaaggt aaggtgtctg tatccaaaca cgttttttag cagaagtaat
    14881 caatttgata aatgaaagct tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag
    14941 ctggatggat gtatcagcaa gcttagattc atctcgatct gcttttttaa agaaataatt
    15001 caaatgatag ccgacctttt tttcttcagg taccgagacg ttccagtctg ttaagaagcg
    15061 aagctggagc aaagaagaag cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc
    15121 gaattttttt gttgtattta catattgatt ggcaatattg aaaccaccgg tataactaat
    15181 ctttccatcg atcacaacga tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa
    15241 tgccctttgt gaggtaacaa atgtatggac atgtccacca tttttgatta gacgattgaa
    15301 atcttttgct tttgtgccat gagagccaaa tgcatcatat aatattcgaa cttccacgcc
    15361 ttctgcggct ttttcttcta aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa
    15421 tgcgtaatat tcgatatgga tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc
    15481 caatttctct tgtccgtctg tgagaagagt gacagaattc attcttgtca gcggcatacg
    15541 atttaatgaa gagaagaagt caacaaattg ctgtttgtct ttatcgccca tgtcaggatc
    15601 gtaatgttca atggtatctc ctctaaaaga ctgaaagttt tctaattctt tcaagtcact
    15661 ttgtcggaga tagaattttt tcttatccgt taatccacgt ccgaaaaata aatataatac
    15721 aaagcccacc ccaggaaggg caaacaatac taagagccat gcccaaatgg ctgctacatc
    15781 tcggggtttg atcaaaatag ccaccaaagc aataagtgca tttaatagat aaagggcggt
    15841 tataatacta gataccacaa aaatgttcct ccttacatac aataacatta gtatagcatg
    15901 atagaagaaa gaagcctacc aaggtgaata tacttttcac tcctttcatg aaaatgtagg
    15961 taaatcgggc taaagtagtt gctttttgta ggttaataag gtaggatatt agtacagttg
    16021 aatatctcct taggggagta accggcaacg tttgttgcga taatatcaac agtaagttaa
    16081 ccaaatggtt ttctggtatt gtatgaaatg gtgagaccta agacaaatgc gtatttgtct
    16141 taggtctctt ttttttcaga caggactagg gccaagatca aaaaatgggg gatgaaattt
    16201 ggaaaacaag gaacaagcac aacaagcggt ctataaagaa acgatcgact cgttgatgca
    16261 gcaaacgcag agctcaaccg atggattgag caccacgcaa gcgaaggaaa aattagaaag
    16321 agatggcttg aaccagctaa aagaaaagcc gaagaagcca acttggaaat tgttcttaga
    16381 aactttcaaa gatgcaatgg ttatcgtatt gctgattgtc gcactgattc agatggtcat
    16441 gggtgactac atcgaattct tagttatttt tgcagtattg atgttgaatt ctataatcag
    16501 tgttgtccaa acaaaaaaag cagaaggttc tttagaagcg ttgaaaaacc tttcagcgcc
    16561 cgatgctcga gtgattcgtg atggtaagga aaaaacgatt ccggcaaacg aacttgtggt
    16621 aggagatatc gtgttgttag acgcaggcga ttatgtacct gctgatggac gtttactaga
    16681 tgcgggatca ttaaaagtag atgaaggtat gctgacagga gagtcgactt ctgcagaaaa
    16741 agaagtaacg gatatcacaa gtacggtacc aatcggagat cgtacaaata tggtattcag
    16801 tggaacgatc gtaacttacg gacgcggtaa attcttagtg acttcaactg ggaataatac
    16861 ggagatcgga caagttgctg gccttttgga atcaacaact gagcaagtca ctccgttaca
    16921 acgaggtctg gataaattca gtaaaaaact aagtttggct attttggcat tgtctttatt
    16981 gattttaggt atccaactgt ttcgtattta tcttggagaa ggtacaggag atatgactgc
    17041 agatatcgtc agcgcagtga tgtttgccgt agccgtagcc gtagctgcta ttccagaagc
    17101 attacagtct atcgtaacaa tcgtgttatc tttgggaacg aataaaatgg caaaacgtca
    17161 tgccattatt cggaagcttc cagcagttga aacacttggt tcgacaagta tcatctgtac
    17221 agataagact ggtacattga cacaaaacaa aatgacagtt gttgactact ttttaggtaa
    17281 tggcgatact ggggatttca caaatgaccc ttcaaaatgg acagctgacg aacgtcgctt
    17341 gatcgaaatc agtgttttag cgaatgatgc atcgatcagt gaagacggta ctaaattggg
    17401 cgaccctacg gaagtagcgt ttatcgactt tagtgaaaaa ttaaaccagc cttatcagga
    17461 aatccgaaac aactacccaa gacaagcaga acttcctttt gactcagatc gaaaattaat
    17521 gtctactgga cacacaattg ataacaaaaa ttacttgttt gttaaaggcg gaccagatat
    17581 cgtcttccaa cgcagtaaaa aagtattgct aaatggtgaa gtcgttccga tgacggatga
    17641 attgatgaaa aaattccaag agcaaaatga agctttttca acacgcgcat tacgtgtact
    17701 agcttttgct taccgtccac ttgataattt tgatttatca tttgaagatg aagacgattt
    17761 gatccttgtt ggtttgcttg ctatgatcga tcctccacgt gctgaagtga ctcaggcggt
    17821 aacagaagca agaagtgcag gaatcaaaac aatcatgatt actggtgacc acaaaacaac
    17881 agctgttgct atcgcaaaac aaatcgggat ttttgaagaa ggaaacctgg ctttgaccgg
    17941 gacggaacta gatgaattga cagaagatca actaaacgaa gttttagaaa aagtcactgt
    18001 ttatgcacgt gtttcacctg agaataaaat tcgtatcgtt cgtgcttggc aaaataaagg
    18061 tcatatctca gctatgacag gtgatggtgt aaatgacgca ccagcattga aacaagcaga
    18121 catcggaatt gctatgggaa cgggtacaga tgtggcaaaa gatgcagcag cgatggtatt
    18181 gacggatgat aactttgctt ctattgtcag cgcagtagaa gtaggacgta atgtgtttga
    18241 taatatcaag aaagctattt cttatctatt tgcaggaaat ttaggtgcga tcattgcgat
    18301 catctttgcc ttagttgctg actggtccaa tccatttaca gcattacaac tgttgttcat
    18361 caatttagtc aatgattcac tgccagcaat tgcattaggt acagaaaaag ctgaacctgc
    18421 tacgatgaac cgcccgccaa gagatccaaa ttcaggaatc tttacggaca aaacattgat
    18481 ttctgtaacg tatcgtggtg tgctgattgg gattgctgtt atctttgctc aatggcttgg
    18541 catgcagcat tcacctgaat tcggtgtagc catgtcattt tcaacattga tttggtgtcg
    18601 gacattgcaa acattcccag cacgttcaaa cacacaaaca gctatccaag cagggttctt
    18661 ctctaacaga agtgtcttac tagctgtagt tgtatgtagc gcgctttact gcttaacaat
    18721 gatcccagga ctgaaagaag tcttctctat cccatcagca tttggattga cagaagtctt
    18781 tacatgtatg ggaatcgcac ttgtgacaat tgttttgatg gaaatcacta aattgatcat
    18841 tgtacgagta caaggaaata aataaaacga atcaacgagg ttgtgagcct gccgtttaat
    18901 tctgagtcac aaggtgcaat ttcaacaaac agcttctcat gtttgttgaa tattgtgact
    18961 tgtgaccgaa gagttggcag gagaacaccg tttaaggaag aagttgtaaa atcagcattt
    19021 tgagctaata ctgagggtca tctgattgga cactgtttat tcggaaagag ggactttgac
    19081 acgactttgg tcacagtccc tttttttatt tatgatagac ggaaagccaa tcattttatt
    19141 attcatcaaa agagatagaa tagataaaag aggagaatga tttattccga aaagtgaaaa
    19201 catataacaa aaaattagag gaaatcaaat gaatgaaaaa gaatgggatg aatttgctga
    19261 agaatattat gaaatccagc aagaatcgca agtaactatt gctgaagatg taaaaaatta
    19321 tctgaaaacg gagaaagtcc tgcctgctga aacagcagca gacgttgctg gaggatctgg
    19381 cagatttctt tccttagcaa aagaaattaa tcgctatgat ttgatcgatt tttcttcagg
    19441 aatgctagaa aatgcaaaaa aagaagcaga aaaaaatgag ttgccaaatg tttcctttat
    19501 caaacagtcg ttagaagaat ttatccaatc aaatgagaca tatgaaatgg tttttacagc
    19561 agcaaatcca gcattggtac atcttattca attagaaaaa ttgatgaaaa agtcaagcaa
    19621 atggtgcgtg ataatcagag tagtggaatc aatcgatgat gttttttatc ctatcgaaaa
    19681 gcaactagga atgatagaag aagatcctaa tacatcgcca gttgtaatgg atcaatttga
    19741 aacatactta attgatgaag gattcaaagt gaagaaaaaa gattttactt attttacaca
    19801 ggaatcaatt actgaagaat tgatgaagct atactatgac gcgtacgcaa aagatcctgt
    19861 tgtgaccagg tacattcgca aactattcag tgaaaaacga gaaatcctta gtacgactaa
    19921 gctgacctat agactgctcc tttggtccgc agataaaaaa agaacctttc acgactcgtg
    19981 aagggttctt tttatgattt tattatattt tgtagtaacg aatatcattt tcgatctttg
    20041 taatcgaaaa aggtacatct gaagtatatt ctgcacattc tccgtgttca ggaagcgtct
    20101 cactgaaaat cgtttcatat tcgtcaacag tgatccgtgt tcgagaatct agcatctctt
    20161 gatgagattg tgcgaagaga tattcttgat agttttcttc cagataacct gtaaaaaatt
    20221 cacttactgc accagaaccg tagctgaata gaccgatgcg atctcctggc tgtagtgatt
    20281 tcgaattttc cagtagagaa gttagcccca ggtataatga accagtgtaa agattaccga
    20341 ttcgtcggct gtaacggatg ctttcttcat agcgagccat aagacgttcc tgattttctt
    20401 cgtctgtttg gtctaatacg ctttgcaatg ccttttttcc catcttagta tacggaatat
    20461 ggaaagcaat cgcctgataa tcttcgagtc ctcgacccga caattcttta tgtcgattcc
    20521 aaactttttg gaatgattcg atatacgtag aattagataa aggaccatca acaacaggaa
    20581 attcgctata atctggacgc cagaaatcat agatatcttc tgtcagaaat acgctgtcgt
    20641 cttcaatcga taaaatacgc gggttttgag tgatcatcat cgcaacagca ccgacacctt
    20701 gcgtcacttc accaccgctt gccaagctgt aacgagcaat atcacttgct atgactagta
    20761 cttttcgttc tggatgattt ttgacatatt ctttcgccat gtgcaaagct gcggtgccac
    20821 cgtagcaagc ctcttttact tcaaaagaac gagcaaaaga ttggattttc agtaaatggt
    20881 ggataattac cgcactcgct tttgaatgat caatcccgga ttctgttccg acgatgacca
    20941 tgtcgattag ctcacggtct ttttctgtta cgattttgct ggcagcattt gctcctagtg
    21001 taatgatgtc ttcatttgca cgattgactg ccatttggtc ttgaccaatc cctatatggt
    21061 attttgcagg atcatcccca cggctttctg ccaactctgt catatctaaa tataaattag
    21121 gaataaaaaa ggaaagacga tcaatcccta ttttcatttc aacacctctt tacttatgat
    21181 aaaaaactta ctttactaag gtatcataaa aagattaaaa gaaatataaa aaagagaata
    21241 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
    21301 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
    21361 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
    21421 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
    21481 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
    21541 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
    21601 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
    21661 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
    21721 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
    21781 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
    21841 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
    21901 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
    21961 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
    22021 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
    22081 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
    22141 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
    22201 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
    22261 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
    22321 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
    22381 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
    22441 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
    22501 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
    22561 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
    22621 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
    22681 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
    22741 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
    22801 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
    22861 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
    22921 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
    22981 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
    23041 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
    23101 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
    23161 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
    23221 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
    23281 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
    23341 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
    23401 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
    23461 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
    23521 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
    23581 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
    23641 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
    23701 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
    23761 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
    23821 agttatttta tttactgcag atcttgctt
//
LOCUS       DAZUKT010000043        23826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000043 DAZUKT010000000
VERSION     DAZUKT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.043"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG816_001651"
     CDS             complement(54..788)
                     /locus_tag="ACG816_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4920529.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG816_001652"
     CDS             1115..1525
                     /locus_tag="ACG816_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4920530.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG816_001653"
     CDS             1518..2219
                     /locus_tag="ACG816_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4920531.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG816_001654"
     CDS             2219..3805
                     /locus_tag="ACG816_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920532.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG816_001655"
     CDS             3824..4045
                     /locus_tag="ACG816_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4920533.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG816_001656"
     CDS             4042..4803
                     /locus_tag="ACG816_001656"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4920534.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG816_001657"
     CDS             5183..5692
                     /locus_tag="ACG816_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4920535.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG816_001658"
     CDS             complement(5767..6306)
                     /locus_tag="ACG816_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4920536.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG816_001659"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG816_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4920537.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG816_001660"
     CDS             7319..8170
                     /locus_tag="ACG816_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4920538.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG816_001661"
     CDS             8198..8833
                     /locus_tag="ACG816_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4920539.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG816_001662"
     CDS             8853..9626
                     /locus_tag="ACG816_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4920540.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG816_001663"
     CDS             10034..10831
                     /locus_tag="ACG816_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4920541.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG816_001664"
     CDS             10809..11222
                     /locus_tag="ACG816_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4920542.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG816_001665"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG816_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4920543.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG816_001666"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG816_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4920544.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG816_001667"
     CDS             15999..16559
                     /locus_tag="ACG816_001667"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4920545.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG816_001668"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG816_001668"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4920546.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG816_001669"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG816_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4920547.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG816_001670"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG816_001670"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4920548.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG816_001671"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG816_001671"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4920549.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG816_001672"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG816_001672"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4920550.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG816_001673"
     CDS             21906..22484
                     /locus_tag="ACG816_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4920551.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG816_001674"
     CDS             22536..23018
                     /locus_tag="ACG816_001674"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4920552.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG816_001675"
     CDS             23135..23509
                     /locus_tag="ACG816_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920553.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6644 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaag
//
LOCUS       DAZUKT010000044        23414 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000044 DAZUKT010000000
VERSION     DAZUKT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.044"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..380
                     /locus_tag="ACG816_001676"
     CDS             45..380
                     /locus_tag="ACG816_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920554.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(637..2220)
                     /locus_tag="ACG816_001677"
     CDS             complement(637..2220)
                     /locus_tag="ACG816_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920555.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2342..2926
                     /locus_tag="ACG816_001678"
     CDS             2342..2926
                     /locus_tag="ACG816_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920556.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2937..3182
                     /locus_tag="ACG816_001679"
     CDS             2937..3182
                     /locus_tag="ACG816_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920557.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3188..3910
                     /locus_tag="ACG816_001680"
     CDS             3188..3910
                     /locus_tag="ACG816_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920558.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4041..4784
                     /locus_tag="ACG816_001681"
     CDS             4041..4784
                     /locus_tag="ACG816_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920559.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4790..5155
                     /locus_tag="ACG816_001682"
     CDS             4790..5155
                     /locus_tag="ACG816_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920560.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            5171..5701
                     /locus_tag="ACG816_001683"
     CDS             5171..5701
                     /locus_tag="ACG816_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920561.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5863..6171
                     /locus_tag="ACG816_001684"
     CDS             5863..6171
                     /locus_tag="ACG816_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920562.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            6302..6586
                     /locus_tag="ACG816_001685"
     CDS             6302..6586
                     /locus_tag="ACG816_001685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920563.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            6723..7523
                     /locus_tag="ACG816_001686"
     CDS             6723..7523
                     /locus_tag="ACG816_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920564.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            7560..7742
                     /locus_tag="ACG816_001687"
     CDS             7560..7742
                     /locus_tag="ACG816_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920565.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7853..8170
                     /locus_tag="ACG816_001688"
     CDS             7853..8170
                     /locus_tag="ACG816_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920566.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            8173..8316
                     /locus_tag="ACG816_001689"
     CDS             8173..8316
                     /locus_tag="ACG816_001689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920567.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(8520..8999)
                     /locus_tag="ACG816_001690"
     CDS             complement(8520..8999)
                     /locus_tag="ACG816_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920568.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            9083..10456
                     /locus_tag="ACG816_001691"
     CDS             9083..10456
                     /locus_tag="ACG816_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4920569.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            10479..11006
                     /locus_tag="ACG816_001692"
     CDS             10479..11006
                     /locus_tag="ACG816_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920570.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10993..11556
                     /locus_tag="ACG816_001693"
     CDS             10993..11556
                     /locus_tag="ACG816_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920571.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11978..12715
                     /locus_tag="ACG816_001694"
     CDS             11978..12715
                     /locus_tag="ACG816_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920572.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(12732..13148)
                     /locus_tag="ACG816_001695"
     CDS             complement(12732..13148)
                     /locus_tag="ACG816_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920573.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            13541..14788
                     /locus_tag="ACG816_001696"
     CDS             13541..14788
                     /locus_tag="ACG816_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4920574.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            15056..17446
                     /locus_tag="ACG816_001697"
     CDS             15056..17446
                     /locus_tag="ACG816_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4920575.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            17495..19330
                     /locus_tag="ACG816_001698"
     CDS             17495..19330
                     /locus_tag="ACG816_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4920576.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            19405..20262
                     /locus_tag="ACG816_001699"
     CDS             19405..20262
                     /locus_tag="ACG816_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4920577.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            20252..21361
                     /locus_tag="ACG816_001700"
     CDS             20252..21361
                     /locus_tag="ACG816_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4920578.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            21700..22500
                     /locus_tag="ACG816_001701"
     CDS             21700..22500
                     /locus_tag="ACG816_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920579.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            22606..23394
                     /locus_tag="ACG816_001702"
     CDS             22606..23394
                     /locus_tag="ACG816_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920580.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
BASE COUNT         8705 a         3222 c         4555 g         6932 t
ORIGIN      
        1 caatttccta ggaaaaatag ttaacataaa aggaatgaat tgttatgaaa caatatttaa
       61 atgaatggaa agcgatagag gggtcattag taacgcaaag aataaaggaa ttacctgatt
      121 gcttagagaa ggatcatttg tttcaaatac gagaaatgtt gaaaaaagaa cagtttgatc
      181 cagaccaatt tttagtagtc gagtatccaa ctattggagt gtactgttgt aaccatatca
      241 atgatggaaa atattttatt attcaggagt atggaggaca gttaactcct ttctatacta
      301 catgggaaat gagtgaagaa ggaatcaata attttccatg tgaaagcatc gaagattcaa
      361 tttcacacac cgaatgttaa ttgattataa ctataaaaaa ggattgaaca ttatgttcaa
      421 tccttttttc aatgaaatgt tttccgccat ttcattgttt ggtgcgattt ctattttatt
      481 atatcaaaat aaagagagat tctaaagaaa gaagagggaa atatataaat caagcgacag
      541 tatatttatt attaaatgct attgtgagaa tataactaat tagtttaatt tgaaagtaaa
      601 tatacgctat aaaagtgcat cttgtgttat aaaaaattat tttattttgt gaacataatt
      661 taacaattct ttctcggaat tgatttcttt tcttaaatag tcttcagact tattaataat
      721 attatctatt atgtctagat acatatcact tttcgattca gctaattcat acagtttttt
      781 taattcttta tcctttaatt gaaaaccctg atccatagtc actgcatttg gatatagaag
      841 cccactataa tgtttacgta aattcacttg gaatttctga ttataaaagt ctaacagatt
      901 agaaataaaa ggcggaaaat tactgttttt gtaaaattga ttatttccat ttaggttgta
      961 tagtaatttg ttaaattgct cctgatttaa tttattgtaa atccatggta atacttccat
     1021 atccttatct gctaacctga atgaactagc atcggtaaga tgttcgatca taaaatctat
     1081 gaacatctct ttttcaccaa aatataaata ttgattatat attgtttcta atgttgaaga
     1141 tggtagcgaa gaaactctca ttttttgctg gagtatagga tcaactagac tatttaagtg
     1201 attttgtaat tcttcattgt tgtttcctag aactgcaagg gaacgtaaac ggtagtaata
     1261 ttctatttct gttagtactt gttgtttcgg tttaaaatca tttattgata tttgtaaatt
     1321 ttgaattgtc tcgtctgaaa aatcatgttg taattctgga tgggtagcta taaaagtaag
     1381 taaaaagaac ccttttgtat acgtgatctg ttgattttca ccaagtgatt tattaatttc
     1441 ctctcttgat tcagctgtga acctatactt tgcaatttct ttttttagaa tatttaaata
     1501 gaattcttta ttatcaaaaa ttagggcatt aagtagtctg aaagtacttt ccctatctct
     1561 atctgcttcc tcatttccat aagataattg aaaaacaaac ttaaaggctg tcttacatag
     1621 gttttctttg gctttttcgc ttagtttatt tagatagcta ttttttagtg cagagtgaaa
     1681 ttcttcaggt gtaacagaca tagcatgtat gcgacttgca tattgtataa tttcgatcgt
     1741 tacttttttt cctaatattg gattttttag gaatataatt tcataggact tccgtataac
     1801 atcctgacaa gtttcttttg ttatatctct taattcccaa gtattaccgt tgtaagtgac
     1861 tacaggatga gctgaataat ttctttgatc cttaatatat tttaaatcct catattcata
     1921 gctatcaatt agctcagttg aactataagc tttttctagt agttccattt cccattttgg
     1981 agagtttgga tcactttttc ttagtctagc tatatcttct aaaattttct tagaagtatt
     2041 atcctcgtaa atatttgcta aatcagataa tttatcaatt aaatctaaaa taagcgcact
     2101 gtaattgaca gctattgcag aacgataatt tccctgataa aaactacttc taacttcttc
     2161 taataatgga ataattctag gatttttaac tttttttatt tcatcatcta atataaacat
     2221 ataaatctcc tttttttatt tgtatgacaa gtttaccata aaaaaaaatt aaaaagcgag
     2281 acatatttat gttgttgtgt tatcataata acataaatat gtagagtgag atgatgaaaa
     2341 aatggatttg aaacaatcgt tttacatctg gttatatcct gataatccta tgtcaataat
     2401 ggctgatatt tttgtagtta gcatcttatt gtatttattt tatctaattt tctttcacaa
     2461 tttaatcggg tacatgattc ttgattggaa aggaatgttc gatatagaaa gaaaaccatt
     2521 tgctaacaat caaacctata ccgtcttcga aagtggagat tgggaagaaa atgaagttga
     2581 tgtgatccat gaaattggat tagctttatc aggaatacaa gatgctgatg ttttggtatc
     2641 aatgacaacg gatactggat acaacgctta tgaggtattt tatcttatgt attttatatg
     2701 gcgtgatggt caagaaatta tcaatggtcg tgctgaaaaa acaatcaatt ctaatactga
     2761 aatttctttt tgtatgggtg aaggggaaat gattgtatat aaagatgggt cagcaaacat
     2821 taaaacagcc tttggtgata ttgaaacaag tattaaggaa cttagggaat atgtgcagaa
     2881 cccatataat tttcaatatt tagcaggaag aataaattta gtttaggagt aaaaatatga
     2941 aaaaaattat aaatcaacca acaaatcact tgataattga aggtgttatt ttcgtaacaa
     3001 tgtattttac tgtaggaaat ggcatctcaa atacaatcat tcaaaaaaat ccacaatacc
     3061 tattatttag tctttatgct tggattgtac aaatggtttg ggtttccata ttgatgctgt
     3121 tgttttattg gatagtacgg gttattcttt tattgttaaa caaaagaaaa aaggataggt
     3181 gaataaaatg aaatcaacta aattgaaggt tgtattgtta tcgacagcag cattaataac
     3241 ccttacagca tgtggtacaa ctaatagtgc taataataac aaagacacaa agtctagtag
     3301 tacgatggtt actaaaaaca gcactacatc ggcatcaaca acaatgtctt caatgactga
     3361 aaataatgct gaaaagtatc aaaaatacga agacattaat aaaggtcaaa agccggatga
     3421 agtttataac aactttatta atacagtaaa aaatgatgaa aattatggga atgatccatt
     3481 gcaaaataaa aatctgacta tcgttcttta caaacctgat tgtccagatt gtcaaaaagc
     3541 agaatcaaaa atcatgcaag aaataaaaga aggatttagt tatgctccaa cattaatgca
     3601 tgatattacc ataaataaac cagcttacga gtattacgca gttgctgtag atattactga
     3661 tggtgtacct gagtgggtac aaaacattcc tggtctggct gatgagaaca atcaatatca
     3721 tacgccaaca gttgcagtag taacaccaaa gaaagttaac ggcacactat actgggatgt
     3781 aatgggaatg acaacaaata caactgatga aatgattgaa agtacaacta aatattatat
     3841 ggatttggga gcatataaat tatatcaaca atcagagcct gttatggacg atcaatccaa
     3901 agttaaatag atctttaggg cgtaagccct atttttttaa aattaagtga taacaaataa
     3961 atgatttctg aaacaaacgt tcttactcaa ctactcgaaa cacacaagct aattaactag
     4021 attataaagg agaatgcgta atgaaagaaa tcagtgcaaa tgaagctcat attccgcaaa
     4081 agaatcaggt tggtatatat aaggtgcttc atggtgaagg aatttataaa atacagttac
     4141 ttaccggtta caataatggt gcaaatggga agtggttaaa ggcaacaagt attcgtgaac
     4201 ttagaagtga agtagaaaaa attaggcttg gtctcacaag tgaagaaata gctaaactga
     4261 agggaaataa ttcttttgta attgaaaaag aaaaggaaaa acatctactt tatcgtgagc
     4321 tgaaaattga agaatactat gaaattgatt tacctgaaaa attaagaatt attgatcaac
     4381 gttcagaaga aaaatggaaa actgaaatat atccaagctt aaatgactac aagacatcag
     4441 gaaagcccat gtataactac acaggctttt taacctacac acaatacaaa tatgcctttc
     4501 gcaaattaaa aggcattaaa caagctgaaa ttgccctgta tgagatgaaa aaagtggata
     4561 gattgaaatt gattcaagat ttagtgcaaa atacatggaa tcaagcgatt gtcgagctag
     4621 aaaaagaaaa tgggaaaatt ttaactaata aatatctgat cgtaaatgaa attgaagaat
     4681 taattggttt attttcatgt gaaaaaggtg aagtcttctt gaagtcatca ccaacctatt
     4741 caattttgga cagtccgtat cagtttatac aggcaaggag atgagttaga tgtctagctt
     4801 gaaagatgac tatatccaag caaaacaaga gtatgaaaaa atacagatgt tgaaatctga
     4861 gaaagtcgaa ttatttttaa cacaaaaatt agcagaggat catattgcta acttagatgc
     4921 taaaacttta gaggaatatg atcggcagtt taatcagata catcacaata tcattttgct
     4981 tgttgagcag gcaaagcgaa ggtttgaaaa agcggaatat gatctattag atcaaggaat
     5041 tgtatgcttg gataagggat tgcgaagata ttatggtgca gagttaaaaa agcaaaatgt
     5101 agatagaaat gcactaaaac atgaaatctt agaattgata atgcaacgaa tttgaaggga
     5161 tagaaagcaa atgtcacaaa acattagtgc tgtaaaacaa ttaatagaag aagatttaga
     5221 gaccacagtt cagagattag cggaacgaat ccattttgca aattatcaaa ccatctctaa
     5281 ctggtcaagc aggaacagaa atccggaaga aattccaatg aaattttttt atcgaatcgg
     5341 aaaagaatat ggtgttgatc cgataaaaat tttcgagaag accatgattt attactatca
     5401 tggaacacct aacgaagtgt tagaacatct tttggaagag gcaaaagaaa aatcatcaat
     5461 attagagcta tcgcaaaatg acaaagttat tcaagtgaaa tgcgttgatc caacaggaaa
     5521 gattattgtc ggttttgata ttgaaagaaa atccaaaata gattttatta cacaattaaa
     5581 aaagataaat aatttagata ttctaacgct tagaccagat catgaacagt tgtttatgag
     5641 ttttaaaaaa caattgaaag aaatacaaaa aagatataac aaggtgtttc ctaatgcgtg
     5701 aaaagctcaa agaaatcaga gttttttgga taattaacaa agaatgtatc atcttatata
     5761 ctcttggatt tcttagcatg atcttgtttt ggtggttata tttaaaacag taattggaga
     5821 gggtcaagag gatttttatc aagataagga tgtggaaact gcatgtggaa taatagatta
     5881 aaaacaggat tgttattaat agttatttcc tgcgctatga tgattggtat gaggatacaa
     5941 agggaacaat cctattttga agtttctgct aacaacgtaa tagaaaaatg ctattacggt
     6001 caacattatt ggagtgagga agtcagagaa aatatagatc gtgaatatgt tcaaagaatt
     6061 gtttgggatg catattctat caaagattat cctaaaagtt taacgagtag attattttac
     6121 agtgaaaaag ataatcaaaa actctcagat ttgatgatga aaaaagtatg actattatta
     6181 cgaaagagaa acagagtgct atattcgagg ggaaaaagcc tacaacaaaa gtgactattg
     6241 gggtgacaag gcgaagcgat taactgcaaa ttttagagtg tatgaatcag agtttataaa
     6301 cttgaccttt cttaatagtg tttttattga ttatgtaatt actacgaaac gcattggaag
     6361 ttattcaagt acctacgcat ccttagttcc aatgttaaac tcgatgagcc gtttcttaaa
     6421 agaaagagaa aagaaagagc tacagtatat caaagaaatc atgccggatt ttgaaatgca
     6481 cgaatggcaa attgctttat cagattggaa attggcgaat aaggttcgta gaataacacc
     6541 ttttcaagcc aagcgctttt tgagagctat gaaatttgtg caataaaaaa agccaccaga
     6601 caagttgatg gagactaaca caattaccaa cagttgtaat tgtgttagct tacaaattaa
     6661 ttatggtgtt tagtcaatcc caaattgtga aatggcacca ataattggaa aggaaaaatg
     6721 aaatgtctga agatttacga gagctgaagt tacgaaagaa gcagtatgat cagtattttt
     6781 ccggtttgtt gaataaacat attgaagaag gacttgatca tgattttgga aaagatgaat
     6841 attgtttata ttgcgaacgt tacaatgaaa aaatgtctga atatctacag ctgaaaaaag
     6901 agatacagaa attagagaaa aaagtacatg cttggaagta taaagattcc atgtctggtt
     6961 atgttgaatc tcaaaataag ccaagaaaaa agaagcaagc gtatgttcta aagatggtaa
     7021 atggggaaga gttaattgta ttagcgtaca gtgaagaaga ggttcgagaa ctcgttgaaa
     7081 cacctgtcag gaaaaaggtt gaaacaataa atgagataga cgacagccaa tacaatagca
     7141 tctatattga aacaagtcat ggtggatata agaaattagc aactttcctt agaagttcta
     7201 aagttgggat aatttctaaa aaagacataa gtgctgttac cgatagtttt tgtactttat
     7261 ctgttcctaa agcttatttc caatacgata gtaaaagatt tgaacgctta gagaaattct
     7321 gtagaccgta tactgtattg aaaactaaaa aatttaataa aactaaaaga gtttctatac
     7381 agttaccgac tggtcaaatc tcattaggtg aaaaagacta tctgataaaa gtaggaaaag
     7441 agttaattat aaaagtaccg gaacacttat ttaaagaaaa ttttgagata cgtgaaacct
     7501 tggtatggaa agaagaaaaa tagtttggaa gacatagaat ggtataaggg gaaattgaaa
     7561 tggcagtaaa taaaatattg caggtactaa tcatagacga atttggcgga ggagaatggt
     7621 ttgatgtgca gtccatagac tttgaagaag aagtcgtagt tatagaaaat atttacggcg
     7681 aagtatattc atattcgctt gaaggtgaga atattgagga tttacggttg attggtgagt
     7741 agaaaaaaac agaaatatca aaatggagat aaaaattaat agcatgtaaa gatagtcatg
     7801 cttgggttaa aaatgttaaa attcaaaaaa atgagtcaga agggataaaa gtatgaaaag
     7861 agaggaatac aaacaaagat taaacgaact gcttgaagag gacgaaacat taactcatgg
     7921 ttcgcctgat gaaattctat acatgattga taatatggtg atctttggtg gatatgaact
     7981 tggtaatagg tcagttgatc ataatatttt agaatttgat gatgtatcat gggaagaaat
     8041 tcttgattgg ggaattttgg cggtacctga aactaaaacc tatattagtg acactatggt
     8101 tccttttttt gaggaattgg attataaaag acttcccaaa aatgaaaatc atatactggg
     8161 aggaaactaa caatgttaag tgacttattt attttactga tctatctagg tgtaagtgaa
     8221 attattgcgt attttttgtt tgtcattgct aattatggta tttccagtgc aatcgttcaa
     8281 atgtttagat taccagtaag tgaactaaaa aaatgatgag attatcagca tatcttttaa
     8341 tgtttttgaa tgtggtcacg ttaatctttg tagtaatcgg tgtgactttc gatttaatgg
     8401 aatagtggga aatctataat ggaaaaaaga gacacgaatg aacatatttt ttgaatcaat
     8461 attgttgatg gtttttgcga tcagcgctta ccagaaatct aaaaaagagt aatgattgtt
     8521 taatcatcac tctttttttg tagggaaatt gaaatatgtg gagcttgtat tggtttagtt
     8581 aggattgctt ggctataagg agctagttct ttatcaaaac catcaaagta ggttttcagt
     8641 agcgatttaa ctgatcgctc atcttgtttg atttggataa tttcagcatc caaatcggtt
     8701 gtgagatcag gagcttttga tgcgattacc tgattttttt tatcttccaa ctctcttagg
     8761 ctgtttaaca agttgtaaaa agaatcattt tgattgttca ttccaacgtg agttgcaatt
     8821 ttaacagaag tttcgtccgt attgtcatca aaagaactca ttggaatatc aagtagtaac
     8881 gaggatgttt cttttactga ctttttcttt acagcatttt ttgtttttcc atcttttttg
     8941 ctctttactc gagatgtttt tggagctagt ttaggtaaat cacttgtttt atcagtcatt
     9001 tattagcttt tcctttctat taatttatgt tattatatta tcataataac ataaaaaagt
     9061 acactttttg ggggcgtagt tattgaaaaa atctaagaga aagattgcca cattgcttat
     9121 gttaatggtg caatttacag ggattgcaat gagtgtaatc acaataaatc cgcaagtgat
     9181 acatgctgat gggatagcta cttatgatgc acaatttaac tgtactactg aaggtagtgg
     9241 gtcaggaaca gatagcgatg gaaacgatac tggaagtggc gctgtaacag gtgatgtagc
     9301 caaaaatatt gaagatattt acaaatacat gaatggagaa tatggcttat caggtgctag
     9361 tattgcaggt attttaggta actggaaaca agaatcaggt attgatcctc gagcagtaga
     9421 agggtattac ggagatggac agagagaagt tgacttcatc aaacaattta ctgatggtct
     9481 gatgggtatt ggctatggtc aatggacagc tgggcgacat acagcattag ttgattggtc
     9541 taagaaaaat aatggcggtt cttggtggac tacagaagcc caattggact ttatgttcaa
     9601 aggtgatggg ggattcgtag gaatattaaa agattatgct ttaaatgctt ctgatgatgt
     9661 cgtagaaaat gcagtaactt ttcataaaaa ctgggaaatt tcagctgatt ccgaatcagc
     9721 tgtaatagct gtacgtggtg aaaatgcaaa agaaatctgg aagtacatga aaagcaaggg
     9781 tatggacggt gcaaaagatg aaagtaagat caataaaata tccggaggtt caagtaccgg
     9841 agatggatcg aataattcat caagttcagc atcaggaaca gtagaagatg cctgtgcaac
     9901 tacggatgac gctaattctg gttctggatc aattgggaaa gaaggaaaaa ttaacggtaa
     9961 aaaaggtcaa gtaatagcga atatgactta tgaagaagct ttgaaaaaat atgggaaagc
    10021 gatcacgcta ccaaaattca aagaaccaga ttggtcaact tcacctttta acggaatata
    10081 tcaaggtcag tgtacagagt tgacgtgggc ttatatgagc caattatacg atgggaatca
    10141 accgactact ggaaatggtg gttatgtatg ggaaagctac aaatcggcag gagcaaaaat
    10201 aacgaacaaa ccaatcgtag gttatgggtt tagtgcttcg gatggatggt tatgggctgt
    10261 tggagatgca gggcatactg gtgtagttat agcggtgttt gaagatggat catttctatg
    10321 tgctaacttt aatgttccac caacccaagc tccaacaagg ggagtttcgg taacgttgat
    10381 tgatggagtg gacggatcag ataatgtgca tttctttagc ggtgtaggaa aagctaaatt
    10441 tgaaatcgaa aaataaattg gaatgaagga cgatatagat gaatagtaaa gtgaaagata
    10501 caattatcat ttctttgctt tctggtatgt tattagcact gcttgttgga atcctattct
    10561 tcaataagca gaatcaggac atagaagctg aaaaacacag tgtgcatatt gaagataagc
    10621 tgacattctt taaagaggtt aaaccaaatg aggtggatgg attacggaaa gatttagaaa
    10681 atgatattca agaaatgttg aatggatcag ttcaaaataa gtttgcagat caatcaggtg
    10741 aagaattttt gagaaaacta tttattgtaa caggtaaacc accaattact gataagagta
    10801 gtgagaaaga aaaaatcgag tactataagc cttttacttt taagctaaca aactttggaa
    10861 ttaagacatc tagtagtggg ttgtatgaag cattgaccga ttttgaagtt caatacaatg
    10921 gaaaaaaagt gtatgatacc acattaacca tcacactaaa taatgataag aaagtgactg
    10981 ggggtgcttt ttatgcaaaa tcatgaaatc actactgcta aaaaggcgat cggaatactt
    11041 tccataattg tgatcctttg ttgtgtggta ctatttcaaa agaaaaatgc ttatgataat
    11101 aatgtcaaag aattggaaga ccttaaaagt aaacaaatcg aagtagtcaa gaataaccag
    11161 aaagtgaaag ctatgcagga agctcaatac aaagaattgg gaaatgctga aattaaggaa
    11221 caaagtagga aattctttaa tttattttac aactggaatt catgggaaaa atatacgaag
    11281 aatatgaagg aaatacaaaa agaatttcct cagatagtaa agtctagtcc tgttgatatt
    11341 tctggcacga tggtaggcac tggtaatagt ccaatatcta gctatactgc tgattactat
    11401 acaacaaatc aaaaaggtaa aatggttgaa ttgattacac agacaagaaa tactccaaca
    11461 acagcaacaa caacaatttg gcgtggagta acaagcatag gagaaaatca attgttttat
    11521 ttggaggatt tagatagcta tgtaagagca gagtaaaata aaaaaagagc taacggtacc
    11581 aacgttagct ctttgtgctg acgatgataa caaaacctac cgaaaaagga ttgtttcatc
    11641 acaaacacaa acagaaaaga attgataaaa aacccgaacc tcagaatttt tattaatcaa
    11701 ttcatttaga ttataataaa ttgagctgaa cattacaata cgtatatgtt gtaaaacgta
    11761 aaaaaagtca aaagtaaata ctttcttaaa aagtcaaata aaactattaa agacaatata
    11821 tatggtttat ttgacataag aaaatggtct agatgataat tgtaatatag aattgtagac
    11881 ttaaataaag cataaaatac cttttatgat aaggtatagc tcttaatttt aagcagtttt
    11941 agatataaga ttgaaagtaa agtgggggag aataataatg gcagatcata aattagaaaa
    12001 gttattagaa gaaacgtgga atccaaaaga atttagtgaa ttttttatgg agaattttga
    12061 aactgactta gcagtgattg ttaaagatgc tcttagagag caagggtatc ctgaaactgc
    12121 aaactatata aacattaatt ttactttgta tactgaaaat aaaggaactt gggatttttg
    12181 ggctacttta gcaaataagg agttaagtga taaatcagac acaggaattc gtaacttttt
    12241 tgaatcaaac agagatgatt atatgtatgc taacaatcaa aataaattga actttagagt
    12301 agagtttgat gaaacacctg aagaatttat agaaagacaa ccgcctaaag aaaatgtagc
    12361 aaaggtattg gaagatcgtt ggaattcaga tgaaatagtt tcaacaatta gcgaactagg
    12421 cggacaatat gaaccgctag tagaagcggt tagagaagag ctaagactga ataagtttcc
    12481 tgatgttcaa aatattgatg tttctcaaat tgaaataaac gtcaagatca cgaataaact
    12541 tgattatggt agttgggcag atatagcatt agaaaaatac atctactcaa cgttaaaaga
    12601 gtttatcgaa aatagaatgg atatcatgta tctacagcat ccacagtatt taaactttgg
    12661 tgttgaaata gctacacctc ttgaagaatg gaaaatggag caagggcttg actagccttt
    12721 gctccatttt tttattttac aaatgaatac agttcgtcgt ttttaccaag aatgattgtt
    12781 tgcccaactt tgagattgtc aggtgatcct tcagcggttt gtaaggagaa cgttccattg
    12841 aacttatcgg ataccgttat aggtagcttt aattctgatc cttcctcatc tttgagaatt
    12901 gctgtttttc gttcatcaaa ataaacagtc tttattaaat aatttttcgt agtgatctca
    12961 gcagatttgt ctttttttgt agtagtacca gtttctttag aattagaggt attttctacg
    13021 actattccat ctttggcatt tgagcttgtg tttatttgat tgaatatttt cagcgacaag
    13081 ataacgctaa gaatagtcaa tatagaaaca agtgtaatta atactgataa tttccttttt
    13141 ttgttcattt tttcttaaca tccttttttg ttttgattag ttagatattc cttatctaac
    13201 aagccttttt aggctattaa atgaagataa accccaaaaa aatattgcaa ttatgacagt
    13261 gcaatggtac tattattata caataaattt tgcgaaaggg gtaggtctaa atcgcaaaca
    13321 aaaagaaatt gaacctagac aaatacaact tgtataccgc aagggaagca agtttgattt
    13381 tgggtaaagg tagaaattat gttacaaaca taatcaacta ttatccaaat cgtttgccta
    13441 aaggttcgtt caagaaagtg ggtgacaaaa cctatattat aacggaagaa ggaatagaag
    13501 cattgcgatc acaaacagaa aataaaaaca aaaagaagga atgaatatga agagaaattc
    13561 gcttggtatg gttttattgg catcagctgt acttgcgggg ttatcagtac cagtaacctc
    13621 acatgctgat gaatggactg caaacacacc ggatcaaatc caaattacaa caggacaatc
    13681 aagttatgcg ttcaaaaaag gagatactat ttggtcaact gtagagaagc ttaaaagctt
    13741 aggttacaag actactgttg ctgagtttgc cgaattgaac aatgttgatt tggcaaaagg
    13801 agaacaacgg actatcccga ttggacgaac tttcaacatc agtaaagata cattaacagt
    13861 taaggaagca gatggcgaag tagttagtca aactgttcaa aaagataaac ctgatcctat
    13921 tgaaaatacg gagcaagaga acaaaaaatc cgagactaca aaagaatcgg aaccaacagc
    13981 aaacacttct gataagacag aaacaagtgc ttcaagtgtt gatggagcaa gttcatctaa
    14041 tacaaataat tctacaagtg agaaagaagc atcacaggga acaagtgaaa gtagcactac
    14101 ggaagctgat actggaacat caacaaataa ctcacaatca acatcaaatg atgcaaatga
    14161 atcaaccagt acatcttcta gtgatcaatc acaaacaggt tcgagtgatg aaactgacag
    14221 ttctacttca ccaagtaaac ccgaacaacc aactaatgtt gtgctaggcg ttccatttat
    14281 tagtcaaggt aacacaatgc tttgcgaagg aacatctttg ctagaagcgt tacattataa
    14341 aggtgttgca acaaatcaag atttaatgac ttttgttaaa tcaatgccac tatctcctga
    14401 caataatcca aataatggtt tttctggtga atggagacac aatgtggatg gaacttatca
    14461 aggtatgaat ccggctcctg tagtttcatg gggtgttgct aatggcggaa atgttgtaga
    14521 tatttctggt tcatcggtac aaggattaaa agatgagatt gtaaatggaa atcctgtagt
    14581 agcgtgggta acttatgcct ttgaacctgc tcaacataaa ccaatgtttt ggggcgatgc
    14641 tatttggaat cgacatgtag tagccgttga tggatttaaa gacggttttt accatgttgt
    14701 cgatcctgtg tttggggcaa gttggattaa tgcaggatca tttgaaacag catataacgt
    14761 atctcaatta gcggtttcgg tacgttaata aaagatatcg tttatatact aagtagttat
    14821 atagtcttta tttgtccttg cttaactact tagaaaactt gttgctcatg gtgggtagaa
    14881 ctaaaagggg tgatgctatg aatactagag cgttgaaaca gcttgctttc aacgaaaaaa
    14941 atgattaata ggtgtggtga ttttaactta ggaatgggcg cgaaggtcat taccacaccg
    15001 acatatcgtg gctcattttc ttcgcctaac tggtataaag aaggagtaac gagagatgaa
    15061 gcatgaaaaa acgataattt taaaatcaaa aataatttat tgttttgcga ctttagtaat
    15121 gatgttatct acgcttataa tgcctttttc agcaataaga gctgaagcag atgtcacaaa
    15181 accgacaagt gcaaaattta tagaaactgg cgtaaaaaca gacggttata ttagggttaa
    15241 tatgccgaat catccaaatg aatggatgat ttcaagtcaa tttaaagatt ctcatggaaa
    15301 tattggatat tgtatggatt cagaattgcc atccccaaca ggaagtggag ccggaagctt
    15361 gaagtacaag ggagctggca gtgatgagtt ctatcgtatg tttaaaggtg gttttccatc
    15421 aaaaacagca aaggaactgg gtgcaggaaa tgatacagaa gcatggtatg caacacaatt
    15481 agtttcatgg gttttagccg gaaactttaa agtttcacag attgtatggt cacatccaaa
    15541 ccataccgcc gcagaaacag ctcgtgtaaa aaaagcattt gaaaaaattt atgactatgc
    15601 taagaatgga aaagatacgc ccaacactga gttttctatc acagcttcta agacagctga
    15661 cgaaggaaaa tatcacactt tcacatataa aacagcatca aataagactg gtaatgcgaa
    15721 gctcactttt acatcagcaa aaccagctgg tatgaagatt tatgatgcag atggtaaaga
    15781 aattactaac aacactgtga aattaaattc aagttttact attaaagtac ctgtgactac
    15841 accaagtggt actttgtcat ttaaaggtac tgctaatgtt agcacaacca atccttttac
    15901 ttttgatgga cgaggtgtgt atcaagatgc cgtagttatg atcactactt cagaaacaaa
    15961 ggactctaaa tctttgagtg ctaaatggac aagagctgtt ggtcaggtaa cagttaagaa
    16021 agttgatcaa gatggaaatg ctttaaaagg agcggagttt actctaactg acaataacaa
    16081 tgctaaatct gttaagacaa ccggagatga tgggttagct acgtttgatt tgcaacaagg
    16141 cttaacatac agcttagaag aaacaaaagt tcctgaaggt tatcaaggtt catttaaaaa
    16201 ggatggtatt actgttaata ccgatggaca agaatttagt tacactgcta cgaacacaat
    16261 taaaaaaggt aaagtaaaag ttaaaaaggt tgaccaagat ggaaaaccat tagctaatgc
    16321 tgaatttacc ttgacggatg attcatccca aacacaagca gaaaaaacag atgaaaacgg
    16381 acttgcaagc ttcgatattg tagcaaacaa gacctacagc cttgaagaga ccggagtacc
    16441 aactggttat acaggcgtat tcaagcaaga aggaattacc gttaaagatg atggacaaac
    16501 ttttgaatat acagctacaa acactttgaa aaaaggttta gtaaaagttc ataaagtcga
    16561 tcaagatgga aaacctttgg caaatgcaga gttcacttta accgatgaaa aaggtgcaac
    16621 acaagtagaa aaaacagatg aaaatggact tgcaagcttc aatattgtag caaacaagac
    16681 gtatagccta gaagagaccg gagtaccagt aggttacaca ggtgcattta agcaagaggg
    16741 tatcaccgtt aaagacgatg ggcaaacttt tgaatataca gctacaaata atttgaaaaa
    16801 aggtgtagta aaagttcata aagtcgatca agatggaaaa cctttagcag atgcagaatt
    16861 caccttaacg gatgaaaaag gtgtaaaaga agttaaaaag acggatgcaa ctggtctggc
    16921 tacgttcaac attattgcga acaaaactta taacttagaa gaaacaaaag taccaacagg
    16981 ttacacaggc gcattcaaac aagaaggaat taccgtcaaa gatgatggac aagtctttga
    17041 atatactgct aaaaacactt tgaaaaaagg tgtgatcaaa gttcataaag tggatgatca
    17101 agggaaagca ttagcaaacg cagaattcac tttgacagac gacaaaggtg taaaagttgt
    17161 acaaaaaacg gacagcaatg gcttggcaac atttaatatt gttgccaaca aaacgtacaa
    17221 aatcgaagaa acacaaaacc ctaatggtta tacagggaaa tggtctaaag aaggcatcac
    17281 cgttgctgac gatggacaaa cctttgaata tacagtaatt aatcaccttg tacctgaaac
    17341 agtaagaaca ggcggaatcg gtacatcagc cttccttctt gtatttctaa ttgcaggatt
    17401 gagtatggtg ctactgtatt atcgagaaca gcacaaagca aaataaaaaa gtgttaatgg
    17461 aaaagaagta aatatgaaaa aagaaggctg acgtatgaaa tcaaaaaaaa ttttttcaaa
    17521 tttaatgcta tttggtactt tgctaggagt gggattgggc gcaagctcaa tctcttctag
    17581 ccttggacta ggggggcaag tagcatatgc acaacaaaac tataattatc aaggaactgg
    17641 aacgattcat atccataaag taacaaatac taatggggat gggaaccctg tctcagatat
    17701 tgataacaaa gggaaagaaa tagctttacc aaatggtgta aaacctcttg aaggcgttga
    17761 gttctcttat gtgaaactta ctaaagatga agctaaatcc ttgggatgga cggatagtga
    17821 tactacaaaa ggtcaacata ttgatgatgc taaagcacga ttattactaa acaaaaaata
    17881 cagtgaagta agtagaatga agactgattc acaagggaat atagacatac ctatacaagt
    17941 aaatacagac gatttaacag ataacttgta tttgattgtt gaaacaggaa cagtcaaagg
    18001 tgtagttgaa gaagcgtatc ctatgattgt taacgtacct gaactaaacg gatcagatga
    18061 tcaatacttt aattatgatg tacatgtcta tcctaaaaac tatttccaat taaactcagc
    18121 agagcttgat aaaaaagacg aagatggaaa ggctcttgcc ggaactgttt ttgaattgcg
    18181 aaaagatgat ggatctgatc aggttgttgg aacttataaa acagacagca caggtaaatt
    18241 aactgtagat ggtttggaat taggtgacta ttatttccta gaaaccgatt tgggaacaga
    18301 tgctaataaa acaacctatt tactagatgg taaaaaattc aaatttagcg ttaaacgtgg
    18361 ggagactgct aaagtagttc ttgatgttac caactacaaa cgacctgtaa ttactaaggt
    18421 aattgataaa gaaaccgttg atactggcga agatgttcaa tatacattga aaccatcaat
    18481 tcctggtaat attggtaatt acgaaaaata tgttgtcaaa gatgttctag gtgcagagtt
    18541 aacttatgat tcatcagaag tgatcgaagg aatgacttta gatactgatt acagtatcga
    18601 acgtgacgga caaacgacaa actatgtctt tactgaatca ggtatggata agcttcagaa
    18661 acaatttgat gcaggggatc gcttctttac aattttgatg aacgctcata ctaatagtaa
    18721 agctaaaatg gatacaccac ttaagaatga tgtttcaacg atctacaaca atggatatga
    18781 acccgaagac gaaacaccag ttgatcacgc ttatacaatc attggtgatc accaattcat
    18841 taaaacagat ggagatacaa gcaaagcatt gagtggagca aaatttgtag tgaagaacaa
    18901 aattgatccg aataaagtta cttacctaaa acaagatcca acaacgaaat ctgttacttg
    18961 gattgcagat aaatctaaag ctactgaatt cgtttccgga tcagatggta agttccaaat
    19021 tgaaggcttg caatatacct atgatgtaga ttttgataaa caaggtaatc caacaatcac
    19081 agataaaaaa atcaatgatt atgctcttga agaagttgtt gctccttcag gatatacacc
    19141 aactagaaat gatgttccat ttgtggtatc gaatgatgat gcttctagcg aagtaacaga
    19201 catcaagaac cttaaaacac ctgaaacagt tcgtacaggt ggaattggaa cagtagtatt
    19261 tctagtggtt gcaggtgtgg caggaacagg gctatatgtt gtctacaaac gagaacaaca
    19321 caaagcttaa tgaaataaaa ttccctagag tgtttttggc tctagggttt ctttttaaaa
    19381 agtgtgtgaa aagtaggtat gagcatgaaa aagaagaata tcaagttgat catcttaaaa
    19441 attgggatgt tgctatgttt aggagtaggt ctatatcctg taattactca ggcttatacc
    19501 tactaccaaa ctcaacaaac actaaactcc tataaggaaa aatatgatga agcttctgaa
    19561 aaggagaatg aagaagtctt caagagatat tcagaagtta ataggaaacg aaaagacaca
    19621 gaacaaacct cagtcacgat tcctgaagat aataacaata ttccgtcaaa tggaaatctt
    19681 cagaaggata aattaggtga attagtgggt gaaattctta taccaaagat taatgtggat
    19741 ttgccaattt atgatggtgt aaacgatttc caactatcag caggagcagg aatattaacc
    19801 ggaactgatt atccaactgg tgggattgga aaccattctg ttatatcagc tcattcaggc
    19861 attcctaata aaaaatattt tacagattta gagttgctca aacttggtga taaatttttt
    19921 attgatattt cagggaaaaa acttgcttat caagtagatc ggataaaaac aattctacca
    19981 accgaatttg aggatttgct tcctgtggca ggaaaagatt atgtaacact tatgacatgt
    20041 acacctgttg gagataattc tcataggtta ctcgttagag gggttagcat accttaccaa
    20101 aaaagtattg ttgagaaaga aaagaagaaa gaagatttaa aggtaaaggc gcagatgaac
    20161 tggtatccta tcgtagttgt aggggtttta ttaataatag ctgtgatagt cattattaaa
    20221 aaaatagtag caaagagacg aggaatgaaa aatgaagaat aaaatactac tttttttgac
    20281 tcttatttgc actttggttt taacaattta tcctgcaaat cagctctatc atcaattcaa
    20341 aacaaaagag aatgtggcgg aaatccaaga gaaaattgag aaaaaacaaa ctccttcaaa
    20401 agaggaagtg gcaaaaaaag tcgaacaaat aaaatcgaac agcggtaacc aaggattaga
    20461 caatactcag aaaacagata aaggagtaga caatactcag gaaacagacg gtggatcatt
    20521 tgatattcgt gaagctgaag gaaaggtcaa tccatacaca ggagatgttt tttcaaaaaa
    20581 tcaaacgaaa gattttgaac catctaccta ttcaggagcg gttggttatg tctatattcc
    20641 aaaaataaat gtgctagagc ttttgtttct aggcgcttca caggagcatt tatatagagg
    20701 tgtggcacaa atgagtgcta ctagtctacc tttatcaggt aaaggtcatc aaacagtaat
    20761 agcaggtcat cgtggcggtt atacgtcacc acaatttctt tatgttgatc aattagtagc
    20821 cggagaccac atttatatag attatcttgg tgaaaaagca gtttatacgg ttttccgaac
    20881 agacgttatt agaaattacg gtcagaattc aacactgact gatattcctg atttagatat
    20941 gttaacactt tatacttgtc atccatatcc tcagaactac gatcgtttat tagttagaag
    21001 tctaagagat gaaaaagcaa aatacatcac gccacaagta acagttggca ataccgcata
    21061 cgctgatgcc attgatagcg agtccgtttc agataagaca gaaactgtta catcttctaa
    21121 tgatttattt gaagatgttg atcgtgccaa caaggaatta gatggaaata atgatcttaa
    21181 tattccggaa caaggtagcg tagagatgca agacagaacg tttgctgaaa aggcaagttc
    21241 aatatttaca ggaattacca ataaacatca gaaaatgctt ttagtcattt ggactgtaat
    21301 aggagcatta gcaagtctaa aggttcttat tcttttaatc aaagagtttc gaaaagcata
    21361 atgaagatta taaaaattga tagagtacag taaaaaggga ttgatactat tattgatctc
    21421 ttttttattt tgaatagagc tagattcact tttaaataaa atcaaaactg atcttaattc
    21481 taaagaaaaa gtcaagaaaa aaatacaata aattttttta tcagaatatt tatcagaata
    21541 ttattagaaa agataaactc aaaagcttaa tttaataggg ttattggttg tatttagaaa
    21601 taatgtatgg aaaagctata aagcaataga taagatgtag aaaatcttat ttgtgaatga
    21661 aaaacaagtc atcgtaaaat attaatagga ggtgtagtaa tggtgggagt atcaaaaaca
    21721 gctttagact cattagctaa actatttagt agcaggtttg atggtgcaga atttgttgaa
    21781 tatctaagcc aaaacgaaag tggaaaaact ctgatcgaaa caatgaaaaa agactatttg
    21841 aatgaaaatg actttccgac atggacgaat attgaagatg ctacatggga tgtagtatgt
    21901 acgatatatt ctccttgggt aacgattgct gattttgttt tagataaaat tggaggggat
    21961 acataccctg atgccaaatc tcttgctaca agtgacttta aaacgttcta tggttatcta
    22021 ataggtctaa taggcaatgg aatagatttg aaagttacgc caaaacaaac acctgaagaa
    22081 attgaaaaag aaaaaggaga ggttcaaatg gcttatgcaa atgacagaga aaaagtaaaa
    22141 ttattggaag gtaaattatc tgaagaagaa cttggaaaaa tggtgcaaga aatcaatgaa
    22201 gacagcaact attttcctga attgaaggta tatcaaaatg acgaagattt ctttaatgaa
    22261 atgtttagta atagtccatg ggaagcagta agtaaaacaa atttaggaga atggaacttt
    22321 caagatgatc tagttgcatt tagcaatgat gggtatttac gatcatattc agaaagtgag
    22381 tatagagctg aattaaaaac gtatgaaacg gatattctga tgagctattt aaatctatta
    22441 gagagtggtc agggagagtt aagagataaa gttgaagaac tattaaaaga agagctatag
    22501 ggattatttc aacataaaca caagcagaaa actaccaaac aaaacgtacc atgcaaaaag
    22561 tcggttaaac cggctttttg ctctattatg agaggagatc aaaccatgag tgcaacaacc
    22621 gatatggctt tagaagcagt aataaaagat aatacaggaa atgtcgaaaa tgtagaagac
    22681 tttagcgata ttgcaatcaa tctttcagca tggatttcag ctgtaggaga agaaaatatc
    22741 gaatttgaga tcaagaatat ggatgactta aaagcaaaat ttccttttat tgcatcgaaa
    22801 ctaagtcaag ttgccgagga taaatcgtgg aatgaattag ttagtttttc cgaaaaaatt
    22861 tatgatatta cgcaagaaga tgagtgtgtt tcatgggaaa tgaataatca atacaagaaa
    22921 attgacgctt tcgtcaaatc acgtatagaa ggaacagctt ttgagtttga aggtatagat
    22981 accgcagata attggttaaa taaagaatcc tttattaaaa gtatagcgga tgatcttgaa
    23041 aaacaatggt ctaaggaaga ctatgcagag tacctaaaaa cagaaagaga tacagaagtg
    23101 aaagaaaaac tcactgaggt atgtttggat gaacatattg atcccaatat cgtaaaacaa
    23161 gaagactatg aagtggttat tgataaagtt cctactgatt ttgaggaagt tgtaaagtat
    23221 atagttgaag aagcattatc gcaatactct tcagatgagg aagtggacga agaaactgtt
    23281 cgtaccagag tttggcaact ggtttttgat gacaaagatt ataaatattt gagtgatcaa
    23341 aactcggtta attggagtgt acgattatta attgatcaaa atgatattga ataaaataga
    23401 gctgaaaagc tcta
//
LOCUS       DAZUKT010000045        23379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000045 DAZUKT010000000
VERSION     DAZUKT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.045"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..2187)
                     /locus_tag="ACG816_001703"
     CDS             complement(43..2187)
                     /locus_tag="ACG816_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4920581.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="ACG816_001704"
     CDS             complement(2544..3917)
                     /locus_tag="ACG816_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4920582.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="ACG816_001705"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="ACG816_001705"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4920583.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="ACG816_001706"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="ACG816_001706"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4920584.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="ACG816_001707"
     CDS             complement(5365..6378)
                     /locus_tag="ACG816_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4920585.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="ACG816_001708"
     CDS             complement(6459..7058)
                     /locus_tag="ACG816_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4920586.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="ACG816_001709"
     CDS             7244..7945
                     /locus_tag="ACG816_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920587.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="ACG816_001710"
     CDS             7955..10666
                     /locus_tag="ACG816_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4920588.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="ACG816_001711"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="ACG816_001711"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4920589.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="ACG816_001712"
     CDS             complement(12805..13986)
                     /locus_tag="ACG816_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4920590.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="ACG816_001713"
     CDS             14238..15761
                     /locus_tag="ACG816_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4920591.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="ACG816_001714"
     CDS             complement(15830..16558)
                     /locus_tag="ACG816_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4920592.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="ACG816_001715"
     CDS             complement(16571..17851)
                     /locus_tag="ACG816_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4920593.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="ACG816_001716"
     CDS             complement(17992..18594)
                     /locus_tag="ACG816_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4920594.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="ACG816_001717"
     CDS             18839..19045
                     /locus_tag="ACG816_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920595.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="ACG816_001718"
     CDS             19357..20076
                     /locus_tag="ACG816_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4920596.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="ACG816_001719"
     CDS             complement(20157..20840)
                     /locus_tag="ACG816_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4920597.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="ACG816_001720"
     CDS             21058..23244
                     /locus_tag="ACG816_001720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4920598.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7778 a         4212 c         4583 g         6806 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaa
//
LOCUS       DAZUKT010000046        23358 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000046 DAZUKT010000000
VERSION     DAZUKT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.046"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..995
                     /locus_tag="ACG816_001721"
     CDS             138..995
                     /locus_tag="ACG816_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4920599.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1110..2030
                     /gene="pstC"
                     /locus_tag="ACG816_001722"
     CDS             1110..2030
                     /gene="pstC"
                     /locus_tag="ACG816_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4920600.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2030..2914
                     /gene="pstA"
                     /locus_tag="ACG816_001723"
     CDS             2030..2914
                     /gene="pstA"
                     /locus_tag="ACG816_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4920601.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2929..3735
                     /gene="pstB"
                     /locus_tag="ACG816_001724"
     CDS             2929..3735
                     /gene="pstB"
                     /locus_tag="ACG816_001724"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4920602.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3751..4509
                     /gene="pstB"
                     /locus_tag="ACG816_001725"
     CDS             3751..4509
                     /gene="pstB"
                     /locus_tag="ACG816_001725"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4920603.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4522..5199
                     /gene="phoU"
                     /locus_tag="ACG816_001726"
     CDS             4522..5199
                     /gene="phoU"
                     /locus_tag="ACG816_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4920604.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5437..5736
                     /locus_tag="ACG816_001727"
     CDS             5437..5736
                     /locus_tag="ACG816_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920605.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5777..6469
                     /locus_tag="ACG816_001728"
     CDS             5777..6469
                     /locus_tag="ACG816_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4920606.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="ACG816_001729"
     CDS             6466..7233
                     /locus_tag="ACG816_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4920607.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="ACG816_001730"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="ACG816_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4920608.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="ACG816_001731"
     CDS             9198..9518
                     /locus_tag="ACG816_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4920609.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="ACG816_001732"
     CDS             9518..9868
                     /locus_tag="ACG816_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4920610.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /gene="hprK"
                     /locus_tag="ACG816_001733"
     CDS             10035..10973
                     /gene="hprK"
                     /locus_tag="ACG816_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4920611.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /gene="lgt"
                     /locus_tag="ACG816_001734"
     CDS             10988..11818
                     /gene="lgt"
                     /locus_tag="ACG816_001734"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4920612.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="ACG816_001735"
     CDS             11862..12887
                     /locus_tag="ACG816_001735"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4920613.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="ACG816_001736"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="ACG816_001736"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4920614.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="ACG816_001737"
     CDS             13965..14405
                     /locus_tag="ACG816_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4920615.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="ACG816_001738"
     CDS             14408..15010
                     /locus_tag="ACG816_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4920616.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="ACG816_001739"
     CDS             complement(15078..16184)
                     /locus_tag="ACG816_001739"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4920617.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG816_001740"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG816_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4920618.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17731..20076)
                     /locus_tag="ACG816_001741"
     CDS             complement(17731..20076)
                     /locus_tag="ACG816_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4920619.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20355..21257
                     /locus_tag="ACG816_001742"
     CDS             20355..21257
                     /locus_tag="ACG816_001742"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4920620.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21470..23245
                     /locus_tag="ACG816_001743"
     CDS             21470..23245
                     /locus_tag="ACG816_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4920621.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7443 a         4142 c         4926 g         6847 t
ORIGIN      
        1 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
       61 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
      121 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcca
      181 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      241 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      301 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      361 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      421 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      481 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      541 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      601 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      661 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      721 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      781 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      841 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      901 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      961 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
     1021 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1081 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1141 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1201 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1261 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1321 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1381 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1441 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1501 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1561 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1621 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1681 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1741 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1801 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1861 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1921 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1981 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     2041 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2101 tttagcagct ttactacttt atatcttagt tcggggaata ccgcatattt catggtcctt
     2161 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2221 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2281 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2341 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2401 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2461 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2521 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2581 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2641 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2701 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2761 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2821 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2881 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2941 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     3001 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3061 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3121 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3181 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3241 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3301 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3361 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3421 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3481 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3541 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3601 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3661 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3721 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3781 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcaatctaag tttcaatcaa
     3841 ggggaaatca ctgcgatgat cggtccttct ggttgcggga aatccactta tctgcgttct
     3901 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3961 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     4021 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4081 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4141 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4201 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4261 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4321 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4381 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4441 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4501 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4561 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4621 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4681 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4741 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4801 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4861 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4921 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4981 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     5041 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5101 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5161 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5221 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5281 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5341 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5401 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5461 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat
     5521 gaagtacctg tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca
     5581 ggaagactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5641 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5701 ggagatgaat ggcagctagt agcaaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
    17761 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    17821 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    17881 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    17941 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    18001 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    18061 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    18121 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    18181 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    18241 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
    18301 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    18361 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    18421 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    18481 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    18541 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    18601 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    18661 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    18721 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    18781 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    18841 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    18901 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
    18961 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    19021 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    19081 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    19141 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    19201 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    19261 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
    19321 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    19381 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    19441 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    19501 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    19561 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    19621 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    19681 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    19741 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    19801 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    19861 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    19921 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    19981 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    20041 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    20101 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    20161 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
    20221 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    20281 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    20341 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    20401 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    20461 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    20521 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    20581 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    20641 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    20701 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    20761 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    20821 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    20881 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    20941 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    21001 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    21061 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    21121 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    21181 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    21241 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    21301 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
    21361 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
    21421 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    21481 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    21541 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    21601 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    21661 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    21721 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    21781 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    21841 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    21901 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    21961 tatttattac cgtaaatata gttccagcaa aaaaatgctg ccgtggtttt tagggctagc
    22021 tgtttctttt cctttctttt ggttcattcg cgaagattcc atctggcttc ttccttttac
    22081 tgtagtcgcg ttaggcatca gtttcgtcca gatcttgcta ggacaagact tttctttcaa
    22141 agaagcaaag aagttaaaag aacgaccatt tctcctttcg aaacaagaat ggctgaaact
    22201 ttcattgttt ttactccctc tagtcggttt ctccatcgtc tatcagtgga tcgaatataa
    22261 aaacgagaca acatatagta ttgccgccat caatgaccgt tcgagtggcg aatttggacg
    22321 tttgatgaaa cagctgatcc gaatagatga cgaaacgaac ttaaataaag aaaacagtga
    22381 gatatgggtc tcgcatgctg cattaaataa agcattagac gcatctccta catttcatgc
    22441 tgtttctact cgtattgacg aattgtatac gactcatgcg tggacccaag gtggaaaagt
    22501 aaaagaatta ccaggagata tcattttttg ggcattgcga gatgtggtaa gcgaatctgg
    22561 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca atcaagaatt
    22621 agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa gaattctatc tgatgggatc
    22681 aggggatgga aagacgatga atgatctgcc aatcgtattt gcttttctga aatcaggata
    22741 tgcctatgca tggcgataca aagatttcca tcaagggggg aattatagtt tcggtacgca
    22801 agaacaagtc gaacaggccc aagaattatt gaatcttcca ctaatggata attggatcga
    22861 tagcagtgat ccgagaacac ctgatttcca gttgacaaaa acagcaaaag tcgcgaatgt
    22921 catgataaaa atttaccaag ttctggccat tcccgtgctg attgcttcaa tagcagggat
    22981 attacttatg atactaggaa ccgcatttgc aaaagctgat cgtgcctatt atagttcctc
    23041 tttgattgta gtcaccggat taatactgac acaattggtt tttcttttcg gcgtctcgtg
    23101 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    23161 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattgaatct
    23221 gaaaaaagct ttatcttcca aataaaaaca tactaagatt agaggagaac atcattgacg
    23281 aatgatgttc tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga
    23341 acaaagcctt gaggacta
//
LOCUS       DAZUKT010000047        23116 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000047 DAZUKT010000000
VERSION     DAZUKT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.047"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..2713
                     /locus_tag="ACG816_001744"
     CDS             242..2713
                     /locus_tag="ACG816_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4920622.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2816..4552)
                     /locus_tag="ACG816_001745"
     CDS             complement(2816..4552)
                     /locus_tag="ACG816_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4920623.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4549..5253)
                     /locus_tag="ACG816_001746"
     CDS             complement(4549..5253)
                     /locus_tag="ACG816_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4920624.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5384..5887
                     /locus_tag="ACG816_001747"
     CDS             5384..5887
                     /locus_tag="ACG816_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS4920625.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5847..6185
                     /locus_tag="ACG816_001748"
     CDS             5847..6185
                     /locus_tag="ACG816_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4920626.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6206..7624
                     /gene="ffh"
                     /locus_tag="ACG816_001749"
     CDS             6206..7624
                     /gene="ffh"
                     /locus_tag="ACG816_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4920627.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7736..8314
                     /locus_tag="ACG816_001750"
     CDS             7736..8314
                     /locus_tag="ACG816_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4920628.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8318..8839
                     /locus_tag="ACG816_001751"
     CDS             8318..8839
                     /locus_tag="ACG816_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4920629.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8918..9472)
                     /locus_tag="ACG816_001752"
     CDS             complement(8918..9472)
                     /locus_tag="ACG816_001752"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4920630.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9725..10000
                     /gene="rpsP"
                     /locus_tag="ACG816_001753"
     CDS             9725..10000
                     /gene="rpsP"
                     /locus_tag="ACG816_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4920631.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10012..10263
                     /locus_tag="ACG816_001754"
     CDS             10012..10263
                     /locus_tag="ACG816_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4920632.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10388..11179
                     /locus_tag="ACG816_001755"
     CDS             10388..11179
                     /locus_tag="ACG816_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920633.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11349..11870
                     /gene="rimM"
                     /locus_tag="ACG816_001756"
     CDS             11349..11870
                     /gene="rimM"
                     /locus_tag="ACG816_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4920634.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11860..12621
                     /gene="trmD"
                     /locus_tag="ACG816_001757"
     CDS             11860..12621
                     /gene="trmD"
                     /locus_tag="ACG816_001757"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4920635.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12757..13104
                     /gene="rplS"
                     /locus_tag="ACG816_001758"
     CDS             12757..13104
                     /gene="rplS"
                     /locus_tag="ACG816_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4920636.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13477..14139
                     /locus_tag="ACG816_001759"
     CDS             13477..14139
                     /locus_tag="ACG816_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920637.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14218..17445
                     /gene="fss3"
                     /locus_tag="ACG816_001760"
     CDS             14218..17445
                     /gene="fss3"
                     /locus_tag="ACG816_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4920638.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17559..17873
                     /locus_tag="ACG816_001761"
     CDS             17559..17873
                     /locus_tag="ACG816_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4920639.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17886..18260
                     /locus_tag="ACG816_001762"
     CDS             17886..18260
                     /locus_tag="ACG816_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4920640.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18270..18614
                     /locus_tag="ACG816_001763"
     CDS             18270..18614
                     /locus_tag="ACG816_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4920641.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18685..20037
                     /locus_tag="ACG816_001764"
     CDS             18685..20037
                     /locus_tag="ACG816_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4920642.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20097..21239
                     /locus_tag="ACG816_001765"
     CDS             20097..21239
                     /locus_tag="ACG816_001765"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4920643.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21264..21518
                     /locus_tag="ACG816_001766"
     CDS             21264..21518
                     /locus_tag="ACG816_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920644.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21774..22958
                     /gene="mobT"
                     /locus_tag="ACG816_001767"
     CDS             21774..22958
                     /gene="mobT"
                     /locus_tag="ACG816_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4920645.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22955..23092
                     /locus_tag="ACG816_001768"
     CDS             22955..23092
                     /locus_tag="ACG816_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4920646.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7685 a         4108 c         4972 g         6351 t
ORIGIN      
        1 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
       61 gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc
      121 tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt
      181 tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca
      241 catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga
      301 taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga
      361 acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca
      421 aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt
      481 gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac
      541 aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt
      601 aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga
      661 ccttgtaata gatgacattg ttattttaga gactggatat caaattccag cagatgcaga
      721 ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat
      781 aaccaaagaa ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta
      841 cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa
      901 gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg
      961 gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa
     1021 tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc
     1081 tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa
     1141 gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct
     1201 ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt
     1261 ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta
     1321 tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa
     1381 aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag
     1441 tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca
     1501 attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt
     1561 ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt
     1621 aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt
     1681 caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa
     1741 tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc
     1801 agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga
     1861 acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga
     1921 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     1981 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     2041 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     2101 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     2161 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     2221 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     2281 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     2341 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     2401 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     2461 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     2521 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     2581 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     2641 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa
     2701 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     2761 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     2821 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     2881 taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     2941 aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     3001 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     3061 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca attagatttt
     3121 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     3181 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     3241 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     3301 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     3361 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     3421 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     3481 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     3541 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     3601 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     3661 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     3721 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     3781 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat
     3841 acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat
     3901 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     3961 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     4021 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     4081 gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     4141 tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc
     4201 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     4261 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     4321 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     4381 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     4441 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     4501 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg
     4561 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc
     4621 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     4681 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     4741 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     4801 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     4861 ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg
     4921 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     4981 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     5041 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     5101 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     5161 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     5221 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     5281 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     5341 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     5401 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     5461 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     5521 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     5581 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     5641 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     5701 cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg
     5761 tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga
     5821 atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc
     5881 tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt
     5941 atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag
     6001 tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc
     6061 ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag
     6121 ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag
     6181 aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca
     6241 ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat
     6301 gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt
     6361 tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc
     6421 acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc
     6481 agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc
     6541 aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg
     6601 tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt
     6661 aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat
     6721 cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac
     6781 ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt
     6841 gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa
     6901 cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga
     6961 tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa
     7021 attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc
     7081 tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta
     7141 cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa
     7201 tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa
     7261 aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga
     7321 tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga
     7381 tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga
     7441 agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa
     7501 aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact
     7561 aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa
     7621 ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta
     7681 tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag
     7741 atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt
     7801 tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg
     7861 attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt
     7921 cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa
     7981 aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt
     8041 gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa
     8101 aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga
     8161 tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta
     8221 tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga
     8281 agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg
     8341 tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga
     8401 cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat
     8461 catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc
     8521 agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt
     8581 cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat
     8641 ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga
     8701 atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg
     8761 aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt
     8821 gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc
     8881 ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa
     8941 aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac
     9001 cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg
     9061 gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc
     9121 cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag
     9181 gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag
     9241 gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag
     9301 caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta
     9361 caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt
     9421 tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt
     9481 tcctccttca aactatctat aataatctta caaaagataa gtaactggag caaataaaaa
     9541 cacttactga caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct
     9601 aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt
     9661 acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata
     9721 aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg
     9781 tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac
     9841 ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg
     9901 gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt
     9961 tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat
    10021 ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag
    10081 ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat
    10141 attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac
    10201 ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag
    10261 taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg
    10321 aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga
    10381 acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag
    10441 ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac
    10501 cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc
    10561 aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat
    10621 gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca
    10681 aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt
    10741 gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga
    10801 aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg
    10861 ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga
    10921 aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt
    10981 agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg
    11041 taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga
    11101 accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt
    11161 ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc
    11221 acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa
    11281 aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg
    11341 gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag
    11401 gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg
    11461 tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca
    11521 aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa
    11581 aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg
    11641 aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta
    11701 aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa
    11761 aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg
    11821 gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact
    11881 ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat
    11941 aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag
    12001 acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc
    12061 tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt
    12121 cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa
    12181 cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg
    12241 acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc
    12301 atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa
    12361 acggattctt attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat
    12421 aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg
    12481 caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt
    12541 gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa
    12601 acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg
    12661 ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa
    12721 gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta
    12781 acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt
    12841 cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc
    12901 aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg
    12961 ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc
    13021 tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct
    13081 cgtatcaaag aaatcagaag ataaacacgg aaaacttcct tggaaatctg aaatttgtga
    13141 aaagtccttg tatatcaagg gctttttctt ttttctctat cacagtttta agccattttt
    13201 ggctgttttg gaccattttt caatcatatt cccttaaaaa ttgtcctaga ttgtccgaca
    13261 ttgactatca gtgtcgtcaa aatagggcaa aatttagtag tcagcttgac gacaaatata
    13321 atacgaaaaa taccaaaaca tttaccgaat ttagcaactt ttgaacccaa gtttctcaaa
    13381 gggtcagcta ttagcaaaaa agacaaaaca tgtacttttc tactaaaaag ttgatgtgat
    13441 aggcttctat ctaaataact attaggagtt tttatcgtga agcaaaatga tggacgtatc
    13501 atttggaaaa atcaaagtga gttaaaactg attttaacaa ttaatgaatt catcgaaaaa
    13561 cacggaatta catctagccg tcaataccag aaaaagctat cagaaaatcc aaattctgca
    13621 ccaagtatgt ggttcattaa caaaaaatat ggatcatggg agaatttatt gattagtata
    13681 ggacgagaaa atactgggta tggaaaatgg gctagaatgt cagaacaaga attattggaa
    13741 atagtagaag cctttattaa gtgcgagaag ataacctctc aacgaatgta cgaacaaaaa
    13801 tcagtaggga agaatattcc ttcacttagt accattaaaa agatgttagg agatatacgt
    13861 ccacttttta aagaaaaaaa tgatggatct cgatttactg atttcgaact gctgttagaa
    13921 ctaaaaaatg aaataatccg attaaaacta caggatgatt tatcaatgac aaagtttcga
    13981 aaactagttc aatccccaaa actaccatca gttgatacaa ttatgaaaag aacgaacaaa
    14041 aattgggagg aattgatggc agagattgga tttgattatc gaagaatcaa aatttataag
    14101 cagagaaaca acctatccaa gacaaagaaa actaaatagt atcactctaa atcagacgtt
    14161 caaaattaaa atttggacgt cttttttatt acccaaaaac gaaaggagat ttattttatg
    14221 aaaaagccaa gatttaagaa ttggcgtttg ctcgcaacac tgagtctatt atgccaaaca
    14281 attggtggat cgcttggccc aacgattgcc tttgctgatg aaattactca tccacaaacg
    14341 gtaactgtgg aattggattt ggctcaccag tatgctgtcg aaggtacatt tagcgatggt
    14401 cgtcccatgt cagaagttac cgttccgcac tatgcagttt acaatggtgt gaagcaagat
    14461 attttctgta ttgagccagg ggtgccgatt tacaatgagt tcactcctgg ttatgagaaa
    14521 aatccattgc ctgatatgtc ggagaaagcg aaattagttt ccgttttatg gaaggaagct
    14581 ggtacggatg tggatacaca tattgtggcc cagaagatga tttggcaaga agttaatggc
    14641 tacacgcttc actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag
    14701 tcaaaaatca atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca
    14761 gtgaaaaccg tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg
    14821 gagtttgatg aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa
    14881 ctagtgatta ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca
    14941 gctaatacag gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg
    15001 gcaattgata agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg
    15061 aagattaaaa aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta
    15121 gactttggaa aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca
    15181 ttggatgaga ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct
    15241 tatacgattg acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg
    15301 acttcaaaaa atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca
    15361 ggaactgatc tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa
    15421 gacagtccaa ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa
    15481 acaccaaaag agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa
    15541 gctagtaatg gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat
    15601 caaaccgtgg ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa
    15661 accactttga caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt
    15721 aaaggagctg aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag
    15781 gcttttaaac cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact
    15841 ttagatgaaa agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa
    15901 gaaaccaaag cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa
    15961 aaggtggatg ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa
    16021 gtcattcgct ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa
    16081 actggcttca atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca
    16141 ggtgccgaag atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc
    16201 aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga
    16261 tttcaaaaaa ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac
    16321 actaagagtg aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg
    16381 gtgaccgttc cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg
    16441 atgctctatg atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac
    16501 ggaggcaaag aattgacaga ccttgattct accgagctag ttgataaatt gagctataac
    16561 ttgcatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca
    16621 aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca
    16681 acgttggcga ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa
    16741 caagttttgg ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat
    16801 gaagcaggtg atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc
    16861 aattgtacgg tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg
    16921 caaaccttta ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat
    16981 gtactggatg gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat
    17041 acgaccaaag aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc
    17101 actaaaaccg tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt
    17161 accttcaaag aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat
    17221 ctaaaagaaa aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca
    17281 gaacaaccgg aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag
    17341 acattcccac aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg
    17401 atctttgcga ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa
    17461 caactaaata ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt
    17521 tttgtatcca aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc
    17581 cagatatggc cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg
    17641 aaggatatgg gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc
    17701 aacgagccga tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc
    17761 aagatcaacc actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
    17821 atcgtgggtt taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa
    17881 agaagatgag attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac
    17941 gattttccgc attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg
    18001 aggtaaaaga acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta
    18061 gtatcccagc aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca
    18121 atccaatcat ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt
    18181 tcattaaggc cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc
    18241 aaaatgaagg aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt
    18301 gtctcgatca atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct
    18361 atgatgaaaa aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgtcg
    18421 aagaagattt tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg
    18481 aagtaaatga aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa
    18541 tgccggatgc cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag
    18601 cagtaaatga ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt
    18661 cgattggagg tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag
    18721 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
    18781 gtcttttatt ttcaagagct tagacaattt gatatttcta agctatcact ttctactata
    18841 gaaatcgttc gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga
    18901 agcacgatgt tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga
    18961 caaaagctag cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt
    19021 gaaggatttt tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc
    19081 aaaatctatt accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga
    19141 aaatatcaag atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa
    19201 ctagtagaaa aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt
    19261 gccaatcgaa ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa
    19321 aatcaagtat gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt
    19381 gggaagactt actttttact cactatcatc gaggctttat taaaatctga tgccgagctt
    19441 tttgttcttg atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc
    19501 tattcccaaa aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact
    19561 cgtagcaagg caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt
    19621 ggacttccac caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg
    19681 acgaaggaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa
    19741 tctggtttct ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg
    19801 attcgcgatc aatttaattt ccgggtggct ttgggtcgta tgagtgaact gggatattcc
    19861 atgatgtttg gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat
    19921 gtagatacgg gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat
    19981 gatttcttaa gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat
    20041 aatagtatgt agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga
    20101 catttttaga tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc
    20161 accagtgtat cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc
    20221 atgatacaag taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc
    20281 aatctctcgg accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg
    20341 cgggaaagcg gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    20401 tcgccgctat tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc
    20461 acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg
    20521 tggaatggca agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc
    20581 ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc
    20641 caacatctga tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg
    20701 ggggacaagt ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca
    20761 aaatcgcatt gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg
    20821 aacaaggcaa tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag
    20881 gcggaacgaa gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga
    20941 acagtgtgaa cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa
    21001 gtgtgcatac cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc
    21061 gaaagttaac acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc
    21121 gagcttctca agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag
    21181 ttccggtcat ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat
    21241 gagttgattg gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag
    21301 aagaactgta ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc
    21361 cgtatgtaat tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat
    21421 tggaacgaat tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa
    21481 aagcagagtt ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag
    21541 gcactgaccg cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg
    21601 acggcgttag ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac
    21661 aaaaaataag caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt
    21721 gtagatgtta actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac
    21781 aaaggaattt agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa
    21841 ataaattagc tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg
    21901 gcgtaaccgc atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc
    21961 ccgagttacc tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg
    22021 cgaggaaaat cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt
    22081 acgcctttta ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac
    22141 atgaagaaga taaaggcgtc cttttagaat taaagggtcg tggttgtcgg cagtttgaaa
    22201 cttttttact cgcccaaaag cgtagctggt atgacttttt cgaagattgt ctaaaagccg
    22261 gtggcgtgat gaaacgctta gacctggcaa tcaatgatcg agttggcctg ttagatattc
    22321 ctgatttaac gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa
    22381 gctatcgttc cggggaactg ttgaaacctg atgataaaga tggtatgggc aatactttgt
    22441 atattggcag tcttaagagt gaggtctatt tctgtttgta cgaaaaagac tatgagcaat
    22501 atatcaagtt agggattcct ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac
    22561 tgaaaaatga tcgagcttat catgcaattc aagatttgtt aaaaggtcgc agtattgaaa
    22621 gtaccacatt ttccattatt aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa
    22681 ggcgaactaa ctggcccttg aatgaacaat gggggcgctt cattggacgg aatcagaagg
    22741 aaattcaatt aacttctgaa ccgaagcctt ataccattga acgaacgttg aactggcttg
    22801 gacgacaagt cgcccctacg tggaagatgg ccaaggagtt ggatcggttg aatcagacaa
    22861 cgtacattca ggatatggta aataatactc gactatcaga ccggcacaaa aagattttgg
    22921 agcaacaaag tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tgatggaaat
    22981 tgtgaaagat attttattgg tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt
    23041 gatgcatgca agtgcagcgg cggaccgagc aatggaaaag aaaggaaagt gatgaaatga
    23101 atttcggtca aaattt
//
LOCUS       DAZUKT010000048        23039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000048 DAZUKT010000000
VERSION     DAZUKT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.048"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2569)
                     /locus_tag="ACG816_001769"
     CDS             complement(<1..2569)
                     /locus_tag="ACG816_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4920647.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
     gene            complement(2791..3675)
                     /locus_tag="ACG816_001770"
     CDS             complement(2791..3675)
                     /locus_tag="ACG816_001770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4920648.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3770..4207)
                     /locus_tag="ACG816_001771"
     CDS             complement(3770..4207)
                     /locus_tag="ACG816_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920649.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4424..5335)
                     /locus_tag="ACG816_001772"
     CDS             complement(4424..5335)
                     /locus_tag="ACG816_001772"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4920650.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5712..6350)
                     /locus_tag="ACG816_001773"
     CDS             complement(5712..6350)
                     /locus_tag="ACG816_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920651.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6354..8336)
                     /locus_tag="ACG816_001774"
                     /pseudo
     CDS             complement(6354..8336)
                     /locus_tag="ACG816_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            8516..9181
                     /locus_tag="ACG816_001775"
     CDS             8516..9181
                     /locus_tag="ACG816_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920652.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="ACG816_001776"
     CDS             complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="ACG816_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFS4920653.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="ACG816_001777"
     CDS             complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="ACG816_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFS4920654.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="ACG816_001778"
     CDS             complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="ACG816_001778"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFS4920655.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="ACG816_001779"
     CDS             complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="ACG816_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS4920656.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21759..22334)
                     /locus_tag="ACG816_001780"
     CDS             complement(21759..22334)
                     /locus_tag="ACG816_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFS4920657.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22334..22933)
                     /locus_tag="ACG816_001781"
     CDS             complement(22334..22933)
                     /locus_tag="ACG816_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFS4920658.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4532 c         3344 g         8170 t
ORIGIN      
        1 tttctgggat agaaagaaca aatttacctg tatcatcaat agttgtttcg gacaaaacag
       61 cttgattttt tttcaatgaa accaaagctc ctgcttcgcc tgtacctgtg attgagcgag
      121 aagctgcatt gatcaatgtg gtacttaatt tagcaggctc aggaggaatc acatcgactg
      181 tcgttttggc agagtccagt tctgtcactt cttggccgcc tcttactttt ttagcagcaa
      241 ctatttgcac ttgatccccc gttgttagtc tttcttcgtt gggtacttga acctcaaaca
      301 aaccgccttg ttcgccttct ccccacggat tatatcctgt cccttcattt tcatctgttt
      361 gtccttgtgt gagagcagta atttctacag gtgatttcgt cgtatctgca taagtaatac
      421 gtaaagtcac ttctacttcg cctgtccaag catctcttgg ctttgcctcg acaccttcgg
      481 gaacaacgac atgcccatag atttttttat ctgcattcgt tggtactctt aaggattcaa
      541 tcatcggaag ctggttattg gcactgattc gattataatt tttcattccg gcacctgtct
      601 gagcaaattc atttgttaaa cctgcactag ggcttgatac aaaattacct aagtcggttc
      661 cattgatcga taagtctgtc atatcagaaa aataatccgg atctttttca aagttatcga
      721 acgttactcc tgcctttcgc cataaagcaa tttctgaatg agcgatcttc aaagaagaat
      781 cgccatttcc ttgaaatacc cagccaccta tgccattagt ctcgcttccg atccgtttgt
      841 tttggaaatc gaaataggta ggagattcta agtctacatt caacacatcc ccactgatga
      901 tgccagtctt atcttcccca gttttcccga ccatgactaa ataagttttt tcgccagcca
      961 taaagttcaa atcaatatct ccagttgtat ctacggtagc tccggaatct gcgcgtatat
     1021 cgatatttga tttttctcca gtaaccgtaa aatcagctga ccctggactt tctagaaaac
     1081 cagaagcgtc ataaaattgg atcgcttggt ttccttgatt gagtctgttc acataagatt
     1141 tgccatcccc tgcatttctt accttcaatg agcctccatt agttactgtt agtttttgcg
     1201 taccgttctc aaaacgaact gcgctagaac gaccactttg cttatcaata tcgatgactg
     1261 atccggctcc ttctacgatc aaagaaagtc ctttccctac aaaacgcatc ccattgtttc
     1321 caggttggtc gccatctcca gccattttga ccgagatttg tccaccactt gatacatgga
     1381 tttgactaga atctccttgc aggcgaaatc cgccagtagt cgctgtattg attgtgattt
     1441 tcccatcatt cgacacagcg actcttgatc ctgagcctct catttggata attccgcgat
     1501 tgttgtcgtt ggtaatattg atctttgtat ttaccgaaaa ttcacttcct gagccactga
     1561 ctttaaatgt gacattcccg ggtagcgtat cgacagcctc tccttcatcg ctgacgccat
     1621 ctgcccgatc atagtcaaga gagacctttg aaccactcaa gatatggagt cctggtgctg
     1681 tttttccact gcccgtctca tcacctgaat ctagaaaacc atagaatttt cctagatatg
     1741 gtccataaag ctgactacca ttgattacat ttagctgata gcttctacaa aaataagctt
     1801 tacccggagc ggcattcatc ccttctttca ctggttggac atccaataca ccggaaacac
     1861 catctaatgt gacagttgca cgaggggcgt aaatcaggcc taatttagaa ccatcatata
     1921 agtcaagtgt cccggtaaat tttatttctc ccgtaaaatg atatctgttt ccccaaaaat
     1981 cggacatata agcaatgata atggcatttc catcgatatt tggtatcaac ccacctggat
     2041 gagctgccgt tcctttaaaa ttctgaaact cgatcgactg aggtgtctca ttgatataga
     2101 tgtgatgtgt gccgaaatta tgagcaagtc cgccaaaatc gataacgata tctcgcttga
     2161 tctccaattc cctgtctaaa gtcaaaggct gagttaaacg aatcttagct actgattcgt
     2221 ctgcgagaag ctgcgtcagt tcttctgttg tggcagcttc gctgtaccca tccgctgaaa
     2281 caactctaac ctgtacctct tcactgctat cagacgtttg ttcagctact tctgaggtct
     2341 cacttgtctt attttcttct tgccttgtat tagaagtagg cgcctcactt tgggattctg
     2401 tagtttcaga agtaattgat tcttctgaaa aagtagtctt gtcaaaactt tgattctctg
     2461 attgcgttga ttcgcttgtt ttctctacat cggcaaaaac ggatgtcacc atcaagagag
     2521 ggaagacaaa caaaatcgac gcaatcagct tttttacata ttgattcatt ctccaccatc
     2581 ctctctgacg ttttgacaat tttattttaa cgttttaatt tatttgtaat tgtactttca
     2641 tatttaaaat atgtattttt atggcaaatt tttctgttag gtgaaaattt cttttttgta
     2701 caaaaaaagg ctgtgacaaa agtcttgtca cagtccctta ttccgaacaa ccgatgtcct
     2761 cctgacaact cttcggtcac aacctccatt ttaacaataa acctgtaaat gtgagggtaa
     2821 cactttgatt gtcacaggta atttatcccc ttcatctcca tcaacattcg tcgtcaattc
     2881 ttcctcatca gctaatgaca caactaattg ctcacaagta agataggcta gatttttcgt
     2941 agattcatct actcctttta ataggtctgg caatgtttta agcgtatcga tcaaagaaga
     3001 atcttttaaa taaaccaaat ggagcttacc atcatccact tttgcatttg gaagtaatgt
     3061 ttcaaaaccg cctactgagt tagttagtcc tatcagcaag atactgctct caatttcttc
     3121 cttctttcca tcaacttcta cgtgaaacga gtaggattgt gtggaagcta actgtttgat
     3181 tcctgacatg aaataagcta gttttccaaa tttcgtcttc ttctccgcat ctacatcatt
     3241 gattgcttca gggatagagc caattgcgac gacattcata aaatagtcgt catttatttt
     3301 tccaatatcc aatggcttga ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc
     3361 tagcggtatc cctaatgctc gagccaagtc attcaccgta cccaatggga aaaatccgaa
     3421 atttggccga tgttcttgtt cagcgattcc actgattcct tcgttgactg ttccatctcc
     3481 gcccatgaca aacacactat gatacccttc cgtagccgct tcgcgagtaa agttttttgc
     3541 atcccctgct ttttttgtat gcaagacgac cacttcatca aacaccgatt ccaattttgc
     3601 tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct gaactggggt ttaccactaa
     3661 taaaactttt ttcatcactt tctttccctt ctgattgatt tctttaatat aacgatagca
     3721 taaagaaaaa aattaggctg tgatgaagta ctgttcatga aacattcatt tattttttga
     3781 caagataaaa tacccatact atgaaactga ggaaaattaa aacaatgata taggaaataa
     3841 aagaaaatga taggcattgt atacctaata aagtaaaaat cttaggtggc agataaaaac
     3901 acaaaaatag caagagaatg taatagcgtt tcttccactt tttgttatca gaccttatag
     3961 cgatcagagg aataccaaat gcataactga aaaactcttg atgtactgtt aatatgtcac
     4021 gaccttgaat aataaatgca acaatccccg ctataattag tgtgcctaat accaaaacgg
     4081 aaaattgatg aatatatttt atatacaaat gatccattgt acttcctgcc tttttatttg
     4141 tgagaaaaac aaatccgtat acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc
     4201 actccattta tctacttcta cgtgcatttt ttaatatcgt tttctttatt taagattata
     4261 cagaaaaata tctagggtgg ctggattcta agtaaaaaaa atttttaaaa taaaaagaac
     4321 taaatcaagt aataattaaa ctactgttga tttagttcta ataaaaagat ttagatgaac
     4381 agttttattg atttaaatag ttcatatttt ttatttatac cgcttattta ctacttgctg
     4441 tactagaatc tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta
     4501 cattagcgtc tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt
     4561 ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt
     4621 tttggttttt caccatttta accacataat attccgtagt ataagttgaa gcattggtag
     4681 aagtgatcac atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg
     4741 ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag
     4801 tatcagttga cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct
     4861 tcacatcttt tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat
     4921 ggaaagattc ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac
     4981 cttcttgaaa ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg
     5041 cagatttcaa ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattggg
     5101 aatcgatttc tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga
     5161 tcaactgttg cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt
     5221 gttcgctgat tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc
     5281 ctgacaataa gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc
     5341 aattctccta cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat
     5401 catcaagaac acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa
     5461 tccagaaagt tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac
     5521 ttcttttgtt accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt
     5581 agaacaaatg ttatcgattt tgtttttctt cttccgctgt tgggatattc aaatcagaca
     5641 cttttatgtt cgattttcca gacatttttt tgttgttttt attagaaaaa gtttgttttt
     5701 tcatttgttt tctaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc
     5761 gtgaccacga taactaacgt accttgggaa gcctgttgcg ataagatttc aacgacttgc
     5821 tccttatttc gtttatctaa tgatccagtt ggttcatcgg ctaaaatgat tttgctattc
     5881 ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata
     5941 ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc
     6001 ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt
     6061 tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga gggcatttct ttttatcaag
     6121 cgcttctctc ttccagttaa atgttttgtt tcttgattaa ataaataaaa ttgccctgaa
     6181 ctgaattgat cattcaatcc taatatattc aataaagtac ttttccctgc tccgctctct
     6241 ccaactacag tcacaaaatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt
     6301 ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct
     6361 ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga
     6421 ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata
     6481 agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct
     6541 ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg
     6601 tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt
     6661 aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca
     6721 gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc
     6781 tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct
     6841 ccgctgagaa tatttctttc ccaatacgta ctgtttttca atgtcagtat ttcaacaacc
     6901 ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt
     6961 ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc
     7021 aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt
     7081 tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga
     7141 taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct
     7201 gatacattga ctaatatact tccgttttta ttcaaagttt catataattc ctgatcggct
     7261 gctgatgcaa aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga
     7321 taaaatacat gatagtcctc accagcgatt tcttttggtg tttcatggga aacagctaac
     7381 tgtgaagaag aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa
     7441 aacaacaatc caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa
     7501 gcgatttttt caactaataa aagagaaatc aaagagatac cctatattcc gctgaacaag
     7561 aaaacgtaca tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca
     7621 ccaatgattc ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac
     7681 aatacttgat gtaatgaaaa gccattattc aacaataaac tgatttcttt attcatcaat
     7741 aagaaaaagg atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga
     7801 taattatata cgtctgtact tgaaataacc gaatacgaat agctattttc aaagtcttca
     7861 aaaatattag tggcataatc agtgtgaaat gcttgattga atcgttccac aaatagtaaa
     7921 atgaaccgtt cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct
     7981 tttattttga tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc
     8041 ttctcgtgag aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt
     8101 atcttgaagt tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa
     8161 gaaagctcag tttctgatga gacattttca aataccatga ttagctgctc atattcttct
     8221 tgcccaataa aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat
     8281 tcatttgtcc gatagagtac ctgtttattt ataataacca aggcaaaacc actcatgata
     8341 ccggtcacta tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct
     8401 tacttaaatg attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa
     8461 atgtataggt catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa
     8521 catcggagaa aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg
     8581 cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg gaaaattctc gtacaatgcc
     8641 agaccttgaa atgctgtcac ttttacatca gactttaggt ctgccgattg attttccttc
     8701 tattgaatca gctaccgaat caatagaacc aacattgccg gatgaacctc ttgttatcaa
     8761 gaaatcttat tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct
     8821 cgtcttttgt ttggtctatc tttttcttat caattatcaa tataaaaatg aaacggttga
     8881 tcgattcaat ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt
     8941 ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt gttcatggta catggctgtc
     9001 ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct gtaatcaaac acaaaggaaa
     9061 acacgtcatg aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat
     9121 cctcatccaa gactatgacg atgccttaaa aagaagccct caggagccag ataggcatta
     9181 agtaggagat gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc aaaaaaaaac
     9241 aggggtacaa attaattgaa aaagctttga gatgaaagtt cctaaataaa aaagttcaga
     9301 atgataggta aaaaaatgac taactattct agtattaggc tatttgtatt attcttctgt
     9361 tatttttata tcaaaaaatc cagaaataac taaatatagg tatttctggc ttatagtata
     9421 atatttacgc cacagccccc aatgattctt tttggaatca tgatcaagga cggacataca
     9481 caaaaatatt ttgcccattc gatccgaata gattaacgac taatctagta agtaatatag
     9541 attggtacgt agtcaaacca gcaaatggaa aagttgtcta ttccacccca agggctttaa
     9601 agacagcatc ttccggcgtt tgataaaaaa tcaaattaaa ggtacccatt aattctgggg
     9661 gtacagttcc aatatcagca gcgcttatca taggtacaag tacttttttc gctccggcat
     9721 cttttgctac ttgtaaacag ttagctaaat tatctacctt aatcatagaa ccaccaattg
     9781 taatatcccc taaaataact aaacttggta atacaggttt atttaaggaa actgagcata
     9841 aagctattat agttgataac gataatgttt ttgtcatacc aatgccattc atatcttgaa
     9901 catgaacgac aaaatctttt gtcgtcaaac taatagactg actaacactt ccataattag
     9961 ctcttaaata tcggtatgcc gaatccatcg cttctcttgc tccagcatct gaattgatcc
    10021 cagtacgttc aaactttcca tttcccgaag tcatttgcgt ttccaattta taggtaccta
    10081 attgcccagt tttgccataa gataccgtgt acaaatgccc tggcttccct aacccttctg
    10141 gaatcaaagt tcctccacct tgttcaggaa cagaaacata ctcttctcgc tcttcttcta
    10201 aatcaatgta tgaaaaattg acatcataaa attccattcc accaattttc tttaattgtt
    10261 cttttacacg acgacgcatt tcaagagcaa atcttagtat ttcttcgaca tcactttttt
    10321 caaatctacc atcaggatac aacagtttca tcatcccaga tacaatccga cgaactgcaa
    10381 tcgtatcccg ctgatttaat tgagaaccaa gtttgaaata tttatcaaaa ctatcgccaa
    10441 aactaacttt tcgaagctca cgcatgactt cagaaaaata atctgtaatg aagccatagt
    10501 catttgtaaa aaaatctggt cgatatttag gaatttccca acctggtaaa taacaatgca
    10561 tccgatccaa aaaggctgaa tccgtcccca taacctccgg aaatggctca aataaattcg
    10621 aagttttcaa tattacatca acgctttgat taatatttcc tacaaaagcc attgatgctg
    10681 aagcattctt ttcttctttg cctctagcaa aagatcctga agccatgtaa tctttcatga
    10741 tttgaacgcc atcattatcc ttaaatttaa tcccagcaac ttcgtcaaaa gcaacacagt
    10801 cccacatacc tacaagtccc attgtacgac tagccatatt atagaaaagg tttgctactg
    10861 ttgtttgtcc acctgaaaca aggattgagt ttggtgaaat ttctttatat acatgcgatt
    10921 tacctgtacc acgtggtcct agttcacaga gattaaaatt attttcaatt aatggaataa
    10981 gtcttgctaa atgaagccat tttacccttt tattaaattg tgtaggttcc ataccaatgg
    11041 accgtagaag taagtcaatc cattcatttg tagtaaactc cttgcgagcc atcttgtaag
    11101 cctcaatgtc taaaccaggc atttggattg gatcaacctt ttttgcaata aaagggctat
    11161 tccaacgatc attctcatca taccaatatt ccatcgaaac aatacaccag atccccccac
    11221 ctaataatcg atcaaatttt ttcggatact cttcactaat ttgaacattt tttaatccca
    11281 ggttagagaa ttctgcttca taacaatctt tcttttcatt taaatgtaca gttactttat
    11341 caataactgt ataaccaccc aattcacgaa tttttgcttt tactttttct gcttcatcag
    11401 gacgcacata gtttcgagca agaatatttt taacattttc cacacctttt tcaaccaatg
    11461 tttcatccaa tgacgaagca tacataccaa gtaaatattc caatacataa acaggtacat
    11521 tcgcaccttc tttaattttc tttgttaaat cttttctgac aatctttccg ggaaagaatt
    11581 cagataactt taaaatttcc tgttgttcca ctagaattcc tccatgtgtt cagtcattat
    11641 aatagcaatt aaagtatatc atacttaacc agtaggcttg ataatacaaa agaaaaagta
    11701 tccacaaaaa attgtggata cctcgttttt atatatcaaa atcaaatgat tctgcaaaag
    11761 gattatctat aatgaatttt attcgctcgg tcaaatcatt tttttgattt tttatcacta
    11821 aataataagt atcttcaact gtatatcgac gatttacaaa ttcaaaaaca aatttagtaa
    11881 accgctgatt tgcatcttct gtttttgaat ccgcaacata gatttcttca tttgagatac
    11941 gttcaccata ttcatcttca aaataaagcc agaaagatgc ggggtcataa acatcactaa
    12001 ttgcattttt ttgatagaat tctaacgtgg ttgataatcc tgtaattctg cggttgctag
    12061 tcattaattc aattgctact ggttcctttg cattgctacc tttggctacc gtaatttcta
    12121 gaacagggac tagcatttct tgaatagatg accctccatg aacgtagctt tgtcccccgc
    12181 cagctttctt aaaaatactt gaagtaatcg ggacacaaat gtttctagtg tcatcatttc
    12241 ctaaagaatc tcccaaagag taatttttcg taccaattat ttcgtattct tcttcactaa
    12301 gaataaaacg acgttctaca cgatcttttt catttctaga tggattttca atcttatcta
    12361 cttcttcaac gtggcttctt ttataaataa atccatgatc agccgtaact ataaaacgat
    12421 agatactgcc atcattatgc aagcgattaa taagtcggga aatttcgctt atcgctaaat
    12481 cacaggcctc aaaaactcga ttttcattcg agtgatcacc tgttctatcc acttgatcgt
    12541 gataaatata aatgactgat ttgccattaa atacttctcg tacctcagcc ttattcatct
    12601 taaccacatc atcataattt aaagctacag catttgcatc tcgttgctgg agaatcgcat
    12661 ttcgattgga taaaccattc gtttttttcc catcaatcag tacttctgtt ccttcattgt
    12721 aagttagttg attgtttggt aataaggcgg ctttaccgaa ttcagtaact gaaggtaata
    12781 cagaaagaaa gctagccatg tttgttgaat acttcttttc tctaactaac ttacgttcta
    12841 aattcatccc taactcatat cgaaaagcat ctgaaataat aactacagtt ttaacagagc
    12901 gtttcattac agcttgatta taaaattttc gaatggatgg acgattctta aattcaaaag
    12961 tttgattcca aatttgtcct gattgatcaa gaaacttcga ataatttgct tcaactaatc
    13021 gatacaattg gccatatttt tgcttttctt ctatatgatc actattccaa ataaatttcc
    13081 gataatgcgt atcaacccaa tacatttcct tctcatattg agttagcaaa ttatccatgt
    13141 tcatcggcaa gcacaatctg tttttatcca caagataata agcatgtttc aataattgat
    13201 actcataaat ataatccgat gcgaaatgca ttttttcgcg ctgctttaaa ctatcaaaaa
    13261 ttgtttgtcc tgcaattttc gttgtagtgt cagaagcctt taatcgttct gcataaaaca
    13321 ttaatatttt tgcatgaatc tcagaaaata cgtctgcttc aattagatca tcaatttcta
    13381 tactgtctaa taatccatca ccatcaatag cctgatatac ttttttagat agctgttcaa
    13441 agctttccat gtaacgtgta tcattcataa actgatccat gaaagtaata atgtttgctt
    13501 tcaaatgtag tatttcaaat cctttgaact tcttcggcat ctcttgatca atttgataat
    13561 aaaaatagtt caaataaaga cacatcgtca gcttttccaa tgttggctgg ctatccgtaa
    13621 agccaaattt catttgaata aattcccaaa atttagctgt taactgatac ttatcaagtt
    13681 cccttaaagg attctcccta ctttctgtaa agctttttag aactacacgc agtaaatcat
    13741 ttaatcgaat acttttcgat ttcaacaaaa cagccattat cattaattca atcgtttctg
    13801 atttattaat tgtcatatgc gtactaaatt caccaaaact tctcatgcga tcctttgcat
    13861 taaagaaatt acgatactgt ttaactgtct gatgaaatct aagtggaata gccaattcat
    13921 tcatgataat cgctaagcgg tctgcactaa attcctctga ataattcaat acactcaaca
    13981 aatgattatc ttcatcttta tcatcccaac gaggaaatgg cgcatagatt aaatagttag
    14041 tttcgggatc ttcatattcc aataaacgct tagttttaaa ttgtgcgtcc ttttctaatc
    14101 gatagatttt tgcattttga atgaccaatg agtcaatttc ttcaagaaaa tcctgatttg
    14161 aatcaaacca aaaaatgatt ttcctacatc cataattagg aaaacgttga tttaattttt
    14221 cttcaatttg ttgcgtattt atatctgtca tcaatctcac ctaatcgttc tctttctata
    14281 ccccttatat tgacataatt tggataattt cgcacgcaat ttaagaataa aaaagaaagt
    14341 tgttagaaaa caactttctt agataactat ttttttggtt ctttgacccc ttttgcaaaa
    14401 atttggaatt tctcgccggt tttaggatca cgctgcaact tatcataatt gacaattaca
    14461 ccatcatcta agtctaattc aattcgtttt aatgctaagt gacccaactt ctcgtcatac
    14521 tcacgaactt cttttaattg cttctgaatc tttacaattt cttttgtcat tgcatttctt
    14581 tcttttgggt tatcacttgt tgaaatttgt tgctgacgca attcgattcg attgtcatac
    14641 gctttttgta gttcgtgtag gtattcagta cgtacttttc ctaattgatc tggtgtatag
    14701 cgatgcaagt acattaacgc tttaaagcca ttttgttttc ctgagtccaa ttgccaataa
    14761 attggtcgat tggagtatgt ttttaaatgg tctttgtaaa aatcttgtac gaagtaacga
    14821 cgaataattt ctcgacttgt ccctttgcca cctaaagtgt cagcgataaa ctggagattt
    14881 tcttcaaggg tttctttacc aaaagtaatc tcaacgaaat caatgaaacg attgacaatg
    14941 tcaatttggc tatccttgaa atactcatca tcggtgatga gtaaaacatt atcagtatca
    15001 ggtttaaatt tttgatagcg ggaaggatcc cattcgccgc cagcgaagat gagaccatct
    15061 tcgtctaggg agtaacgacc gaagatacaa cctattgaat aagaaataaa atccttaatt
    15121 tccctcgcct tatcaggtaa ccttgcagag atatattcat cttgaacctc ttttgttaat
    15181 tctttttcaa gagaataatg attaatgaat atgtcgttca gttcctcttc attcgatttt
    15241 agagtattaa attgattaac caactgccta cttagacagt taaatgcctc tgagagatct
    15301 tttttaaaca tataacttaa caaaatgttt cttctaaact cgtatgacgt ttcaagagca
    15361 ttatagtctt ttctagctat atcgatattt tcatccactt tttctcttat tttttttaat
    15421 tttctctcag atgtaacttt aagaaaaggt agttttccca ttggtccttc atggtaatcc
    15481 aaggtgggag aaataaaagt taaaaagtga ttaactaatt tagagtttac atagccaaat
    15541 atttcttcaa agtcagttat atcctttgag aacagaacag atcctttagt ttcaaaaatt
    15601 gcctgattct gcaatctaaa agatacttgt ccagatgaaa gtgtcgacca agtaattccc
    15661 tttttaaaat agtaattaag attttgtggt ctagatttta acttcccatt ttcattaaca
    15721 taatttttta tttcatctcc atcattctcc cagttaacca cgtattcatt gtttccatac
    15781 cattttctaa aacgaccacc tttagttgta ggaaaccatt tttttctaga ctttttagcg
    15841 tcctctaatg ttttagcatc aaactttatt ttgttaaatt ctacttcaaa ccaataccgc
    15901 aaaaatttat tgttatttcc ggttgccaac ccttgttttg catctgcaac atcgttagtt
    15961 aattttcctt gagcaaaatc agtgattacg ttatcgcttg cccagtaagc aatcggcatt
    16021 cctgggattt ttttgaagtt gtcttgatta gtactataaa aataatcgca ttcaccatgc
    16081 tgatgaattg cttctaacgt tttaattttt tgaacttcca ttccaccttt aaaatctgat
    16141 aattttatat agcttccatc ttgttcattt gaatttttta tcgtaaacgt acaaattgga
    16201 acggttgctt cttcaaaagc tgaatactcc atttgaatca aactagaaat gtttctttca
    16261 ttaattaaaa atgagcgtaa cttttcatag ctagaaatga acatccaaac aaatggcgtc
    16321 ataaaggaag catatccatt atcaatcagc atttggctat tcaatttaat aaacacagaa
    16381 aatagatctt ttttgacatc agaatagtta tcatttacat atttttttaa tttcttatcc
    16441 attttattaa gataaggcgg attagtgact accgctacat atttatcaac tagcatttca
    16501 gccacgtcaa gaatgtgcaa gtatttttct tgtatttcct tcatcccata aagaatatcc
    16561 atattcgtaa attcgttgaa ttttgatata tactctctta cttttcctaa gtattcttta
    16621 gttattgcct gattattttt atcgactaac ttaatgattg atccaagttc tttcccattt
    16681 tcaaattgat caaaaattaa ctctttgtat tcattccagc gttcactata cttatcattg
    16741 ttcaattcac taatacgctc ttgatactcg tctgtcactt taggagaatc aataaactct
    16801 cttaaattcg gcgatagact ttttttcaaa aaaagacgat cgtattgacg ccctttcatc
    16861 ataattgcaa aataagacaa ttgatacgct cttttatcga tttcaagacc atataaattg
    16921 tattcaacga ttgcacgtgc agcatcacgc tcagaatatc ctaacgtaag ataaatttcc
    16981 atcaatacat cgaaggcgta aactaaaata tgcccagacc ccattgcgtt atcgataatt
    17041 tttagttctt ctggttttaa attacttaca gatgttgctt gttcaaaagc atatttatct
    17101 ggaattaagt atttcaactt atttcttaat tcactattgg gataatgatc taaccaatac
    17161 tttcctaaac tattgtcaac catataacga acgacccaat ctgtggtaaa tagttgagtt
    17221 gctgccggaa tatcttgttt tttgacaatc cctttataaa tattcaccac ttgattatgt
    17281 ggctcactat tatagtactg atacaaccac ccaataatct ctacctgtcc accagcttga
    17341 acatagaaat catcctctgg aatacttaaa agatcagcga ttacaccatc ttttttaata
    17401 taagaagctg taaacaaaag ttctgtataa tcttctactt cttcaaatag ctcaggtaag
    17461 taatcattca attgattaca gatttttata aaaatcaatt gatacagctt gtccattgct
    17521 tctgctgaat tatctgtttt ccaagttctg acttgttgct gttcattact agataattct
    17581 tcaaagatat cggcatcata aatatctgta ataatatctg gctcaatttt cccacttatt
    17641 tcactagata atacacgata cgtgtcaggt aaataatcat ttacctccat aaaccgaatg
    17701 gcaatcaatc ggttaaacca cgtataagca acctcttcga ttaactcttg aaaagcttct
    17761 gaataagaag tgacagaagc acgttgtttt agttctgcaa tcaatttgtt tctttgcgct
    17821 atttcttttc cttgaattct tattcctgat ttttcgattt cttgtaaatt gctatcaatt
    17881 ttcttcacat cttcaatttt tgattcatca attcctaaac gttttgcttt agtcattact
    17941 tcatcaatta attttctacg ggcattgacc gcgaaattct ttatcgcttt cttatccatt
    18001 tttctttccc ttctatatgt aacctttgaa aagacaggca cctttttatg cctgtctttt
    18061 cactagaatt gaatcgtgat ttctgtattt tcttgaagtt gttccttcag tttttgatta
    18121 atttcagctg ttaactcatc aatatctgct gctgacttaa tagttttaac atgaacgtta
    18181 agtgttctca ttggtactgt tttctgttga atagtaggtt ttactatcgc tatttcttca
    18241 gcttttgctg cagagtctat ttcatctttt tcagtttcac gaatcaagtt tgctgcttca
    18301 atttgtttac gtgcttgttc ttcttgacgt ctgcggtttt cttcattatc gaaacgttga
    18361 ttaaagatca aatactgttc tgctacgttt aatgataaga aaacaagatc atctatagta
    18421 ttcgcttgtt caatttttgc tttcagttct tgaaattctt ggtcaactat tggtacaaat
    18481 tgatccttaa aatcttttcc ttctaataat ttaaatgttg cttgtttttc ttccgttaat
    18541 ttatttaata tttcctgact ttccaactct aacagttgat tatattgttc aataaattct
    18601 tgaatcaact catttagttt catcatatct gaataaggat cagccttttt caaaatggtt
    18661 ttcatctcat gaatcaattg atcaatttct tggctagaag tttgacgccg acttttttcg
    18721 attaatctaa ttttatcttg tgcattctcc cagatttttt tctgagtact attttgcttg
    18781 tggaaatttt taattttatc atatgtttca gcccagtcga ataattcatc gcgatatttt
    18841 ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa caattacttc aagatatttt
    18901 tttatttgct catattcata agcactaggg taggtataag tgttttgtaa atcaaaataa
    18961 gagcgtaaat tgtctaattg accttttaaa tatttcttga aattttccat tgtatgttca
    19021 ttgttatttt catcaacaat attacgactt tgaaacagtt ctctaccgat ttcgtcaacc
    19081 aatttttttg cttttggcga aatcaccgcg cttggttcaa tttttatctt ttcactttct
    19141 cgtcgttttt gaataatcat tacaatacta tttggatcat ccatccgtga aatttgtaca
    19201 ttgttataaa gtaaatcaat tttaccagac atgaaaagtt tagcgatgca ccattccaca
    19261 tcaagttctg tgaatccata cggttgtttt gtttcaaaac gatcacgaat tgttttcatc
    19321 gtaatcgtta ggtgatttct cttttgatca ttgataaaat tgaaaatttc ctgaactgct
    19381 tgctcatttg atggatcttc aataatttca tttttgcttt ctttcactaa atataagata
    19441 tcttcttttt ctttaggcgc atcaatttgg tttaatttat ggtatacttc ttcagccatt
    19501 ttctgaagtg ctgattggaa ttttgcttta aagtcggagc ctgtcagttg aatttctgca
    19561 ccgttataga aaaattttgc agatttcaac agttcattta attgatcgtt gatacttgca
    19621 cttagttcct gtaattcacc agatttttcc gaacgaatcg cgtcaaagtt ctttactaca
    19681 ctcgaataag tcgttgttaa ataaagattc attctagcat acattgctat atcatcttta
    19741 aattctttat ctgatggcaa ttctacgaat aataagttac gggaactctt aagcgcaagt
    19801 tctgcctctt gacctgctaa atcagagtat ggtgaaataa aattaactgt catttcagca
    19861 tttccagctt gcttgtatgg acgttgatct aacacttgtg cataagtaaa cgtataccgg
    19921 tttttaaatt tatttccacc tgacatgcct aatttttcat aggcaaaact atttcggtct
    19981 agaacgccgt caaaaattcg tttagatagt tctacgatca aatcactatc tgtaatattt
    20041 tgatttttaa tctcacgagt aatttcctgt tcttcatcag tttggaaaat ataactatct
    20101 aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca atgcttcttc tactcgtttt
    20161 ttagtttcca ggcggtcttc gttgatattt cttatcatca agctcgtaat attttcaaca
    20221 tttggtttga tggcttgatc aacatattta atcatgaata aaattcgtaa gacatttacg
    20281 ttaaagttat ctatatcagt tggtctttga atatgagaat tatttgtagc ttgtgtaatc
    20341 actcggctaa ttgctgtatc aatccaacgt tctaacgagt aataaaactg atctaaagaa
    20401 accaaactgg ttgtatcatt ttccttattt cgttcagcgg cttctttaaa cattgctagt
    20461 aatgaacgtt caccagacga taagtttgat ccttgatagc tgtgattacg aatctcggtt
    20521 agaatttgcc ccaataaatt gaattgatag ggaataaatg gataaacttc tgcgtaattt
    20581 tcagcatctt ggaagagctt taattcagga ctatcaacaa agagtaattt atttttcaat
    20641 tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat tggcaaactc ttttttcttc
    20701 aaaatacgtt ctttaattac ttcatcggca tttgctgaag ataaactcac agttgtggca
    20761 aaacgatcct tgatcttaga aaaatcatta tcagaattgt tatcaaagtt tgttacgtga
    20821 tcaatcgctt cctgacttgt cactaatacc caagccttac cttcacaaat tcgacccaaa
    20881 tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat taccgccgat atattgtccg
    20941 acctcatcga ctagaaaggc aatatgatgg tctgcttctt tggaatcaag atagctttta
    21001 actagatttg caaagtcatc aatgcttatt aaataatttc ctgatgcttt ttctaaaaaa
    21061 ttatcaatag cggcttcact cataaaatcg attttaagtg ccgcttcttt gatatcatct
    21121 tggatccaat caaactcatg gcgctcttct tgccattttg ccccattaat ctcttcaaaa
    21181 gcagctttga atgcatcaaa cttttcttct ttaattaacc gacgttcaaa atctgctaga
    21241 tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct ttaaaaaaac attcacgatt
    21301 ccgtttttgt cttttttacc acctgcatca ctttttgaat caatattaaa cagaataaca
    21361 tctgtattta tttgtgagca ccgttccatc gttgccaaag tcagtggatc tttgattttc
    21421 ccatcttctt tgaagtaatc aatggctttc ttgtcattta tttctcgatt tgcgagcaag
    21481 taagaaataa tttttaagaa atgagattta cctgaaccaa agaacccctg aatccaaaca
    21541 cccattttat ctgttggtgt attgatactc cgttggtagg catcaaaaat tgttttaaag
    21601 tgtttttgta gttctttcgt aaccacgtat tcttctaatt cttgttgaac attttcatcc
    21661 tttgcttgac cgaccttaat aactccttga atatttcggt caattggttt tgcaaaaata
    21721 tctttaatta gcatttaatc tcctaccttc catcccaact aaattctaaa tgctcgataa
    21781 taattatcat ctttattggt attaagaatt gtcaaactta attcatcata ttcccctgga
    21841 tacatcatca caactggtac gcgatcaaat acctgatgca ttttatttaa aatagagtgc
    21901 gctctaatta atggatataa ttcgccaatt cctgtcagga acacaactga tttttctggt
    21961 gtatgggcat ccacatattc aaaaaataca ttttcttcct caggtaaatc cattttcaat
    22021 aaacgaccaa tgctttccac taatttttgt aaaccacgtc gttcttccat cttaatcgct
    22081 ttttcagtga aaccgcgttt ttctagatac tctaacataa tcacatagag attataaact
    22141 ttcacttcat aattatcacc aacagatgtt ccagatatat cattaatata atctcttaca
    22201 attaactcgt ctttcggatc gtagtcaaag atataatagc ctacttcatt acctaaacca
    22261 cgattcaata aaaaatcttc tttttgaatt acttcattca acttttccaa atgctcttga
    22321 atcgataatg acattataaa attcctccca gtgctagtac aatatcttta cgattcttgt
    22381 caactaacca ttgaatcaat cgtttatcta ataatatcgg agtcattcgt ccatattctt
    22441 tttcccctct gactaatcct gtttcaatta gattcgtttg ataagcggca ataagccgtt
    22501 taatcgtata atctttccat tttgaaatat cttctgaact tctccgcttc tctgtaaaaa
    22561 aagaacggta atcaactggt gttaattgca aatttccttt acgtagattt tcagcatata
    22621 cctcagtcat aaattcatta aaaagatcat caatcataag gatggaaatc aaattcacta
    22681 acttttgatt atctatattt aactgattga aaagttgcaa tccagcttca tccagttgac
    22741 taactctcct tttcacgttc tgatagattt ctttttgacg gttactgctc actgcaaaaa
    22801 aaatattttc atgttcattt ttctctttaa tttctatatc tgtatatcct tctagtcgta
    22861 attgaatata taacttgaac tctgaaaacc aaaagcgttt agaaactaga ccagcagaat
    22921 attttttatt caatagattg cctcctaatc atctataatt caataagtaa acaagtgttt
    22981 tttgctcatc gttagcattc attttttagt atctactata caatacatat ttattatag
//
LOCUS       DAZUKT010000049        22454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000049 DAZUKT010000000
VERSION     DAZUKT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.049"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..532
                     /locus_tag="ACG816_001782"
     CDS             134..532
                     /locus_tag="ACG816_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4920659.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            516..1210
                     /locus_tag="ACG816_001783"
                     /pseudo
     CDS             516..1210
                     /locus_tag="ACG816_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1804..2691)
                     /locus_tag="ACG816_001784"
     CDS             complement(1804..2691)
                     /locus_tag="ACG816_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4920660.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2876..3715
                     /locus_tag="ACG816_001785"
     CDS             2876..3715
                     /locus_tag="ACG816_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4920661.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3911..4885
                     /gene="sgrA"
                     /locus_tag="ACG816_001786"
     CDS             3911..4885
                     /gene="sgrA"
                     /locus_tag="ACG816_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4920662.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5183..5539
                     /locus_tag="ACG816_001787"
     CDS             5183..5539
                     /locus_tag="ACG816_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920663.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5584..6699
                     /locus_tag="ACG816_001788"
     CDS             5584..6699
                     /locus_tag="ACG816_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920664.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6725..7165)
                     /locus_tag="ACG816_001789"
     CDS             complement(6725..7165)
                     /locus_tag="ACG816_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4920665.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7325..7831
                     /locus_tag="ACG816_001790"
     CDS             7325..7831
                     /locus_tag="ACG816_001790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4920666.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7999..9912
                     /locus_tag="ACG816_001791"
     CDS             7999..9912
                     /locus_tag="ACG816_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4920667.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9917..10918
                     /locus_tag="ACG816_001792"
     CDS             9917..10918
                     /locus_tag="ACG816_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4920668.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11120..11326)
                     /locus_tag="ACG816_001793"
     CDS             complement(11120..11326)
                     /locus_tag="ACG816_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4920669.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11972..12820)
                     /locus_tag="ACG816_001794"
     CDS             complement(11972..12820)
                     /locus_tag="ACG816_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4920670.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12984..13886
                     /gene="gnd"
                     /locus_tag="ACG816_001795"
     CDS             12984..13886
                     /gene="gnd"
                     /locus_tag="ACG816_001795"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4920671.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13888..14889
                     /locus_tag="ACG816_001796"
     CDS             13888..14889
                     /locus_tag="ACG816_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4920672.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14886..15170
                     /locus_tag="ACG816_001797"
     CDS             14886..15170
                     /locus_tag="ACG816_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4920673.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15203..16000
                     /locus_tag="ACG816_001798"
     CDS             15203..16000
                     /locus_tag="ACG816_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4920674.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16014..16847
                     /locus_tag="ACG816_001799"
     CDS             16014..16847
                     /locus_tag="ACG816_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4920675.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16865..17722
                     /locus_tag="ACG816_001800"
     CDS             16865..17722
                     /locus_tag="ACG816_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4920676.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17744..18253
                     /locus_tag="ACG816_001801"
     CDS             17744..18253
                     /locus_tag="ACG816_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4920677.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18290..18691
                     /locus_tag="ACG816_001802"
     CDS             18290..18691
                     /locus_tag="ACG816_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920678.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18803..19156
                     /locus_tag="ACG816_001803"
     CDS             18803..19156
                     /locus_tag="ACG816_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920679.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19190..19633
                     /locus_tag="ACG816_001804"
     CDS             19190..19633
                     /locus_tag="ACG816_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4920680.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19650..20735
                     /gene="uxuA"
                     /locus_tag="ACG816_001805"
     CDS             19650..20735
                     /gene="uxuA"
                     /locus_tag="ACG816_001805"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4920681.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20728..21441
                     /locus_tag="ACG816_001806"
     CDS             20728..21441
                     /locus_tag="ACG816_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4920682.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21648..21797)
                     /locus_tag="ACG816_001807"
     CDS             complement(21648..21797)
                     /locus_tag="ACG816_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920683.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22193..22393
                     /locus_tag="ACG816_001808"
     CDS             22193..22393
                     /locus_tag="ACG816_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4920684.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7743 a         3447 c         4641 g         6623 t
ORIGIN      
        1 cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat gtgttattca
       61 ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg atattttacg
      121 aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta tagaagcaga
      181 agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg aaacattaac
      241 agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt gtgatatgaa
      301 gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg tccaaactaa
      361 tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc cggaacttcc
      421 aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga caatcattaa
      481 aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt gatacagcaa
      541 atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta acaacaaatc
      601 ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa gaaatcaaaa
      661 aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa gaagaaatga
      721 tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc aaggtgccgg
      781 tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat ttgattaccg
      841 gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga gcagattata
      901 ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa gtgattgatt
      961 cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca gctagtttca
     1021 aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa cgttaggtaa
     1081 agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga ggggcttcca
     1141 agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa aggtcgctga
     1201 ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa ctcgaggagg
     1261 ctatctgacg attcaatatt cattaattta aagtttgggt gactattact aactattact
     1321 tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa ctctgaagtt
     1381 acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa aaatatcttt
     1441 cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat ttttagtctt
     1501 ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga agcgatacgg
     1561 caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga ttcatgtgat
     1621 agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca gtgatttgtt
     1681 ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg cctcaaaaat
     1741 aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt gggtcaaatt
     1801 tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt cttttttcat
     1861 cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt cggtagcaat
     1921 tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt tttttaccat
     1981 ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa tgatatcaga
     2041 aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa tctctggatt
     2101 ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt ttccataagc
     2161 ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt tatctgcttc
     2221 ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt ctttccaagc
     2281 agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt ggaaagccag
     2341 catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt ttaattgttc
     2401 cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta cttcctttga
     2461 agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt tttttaatag
     2521 tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg taagtgttcc
     2581 acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata gtaaaattgc
     2641 acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca tcctttcctt
     2701 attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt aaggttgctg
     2761 tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt tacatagttt
     2821 tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat gaaagatgat
     2881 caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg ttttaggaat
     2941 tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga ttggagcaat
     3001 tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg ctagtaaggt
     3061 cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact gggggtattt
     3121 agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc cattatattt
     3181 gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga taacgagttt
     3241 tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct tttttcagca
     3301 tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat tatttgctct
     3361 tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct ttttagcagg
     3421 ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta ttggtgctca
     3481 tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg gaacgcaaga
     3541 acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg ggggaaactt
     3601 tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg ttggtttgtt
     3661 actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc aataaaagat
     3721 gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat taaacgaaaa
     3781 agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa ctgactgtcg
     3841 tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa ttatatggag
     3901 agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc tgagtataat
     3961 tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga atctttcatt
     4021 tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta ctattccagt
     4081 caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat atggttggga
     4141 tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg tacaatggac
     4201 cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg agatcaagag
     4261 taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca cggagaattt
     4321 gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca caagtagtga
     4381 aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca gtgaaattag
     4441 cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa gtagcacagg
     4501 aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca caggaagcat
     4561 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
     4621 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
     4681 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
     4741 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
     4801 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
     4861 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
     4921 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
     4981 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
     5041 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
     5101 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
     5161 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
     5221 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
     5281 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
     5341 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
     5401 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
     5461 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
     5521 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     5581 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     5641 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     5701 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     5761 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     5821 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     5881 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     5941 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     6001 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     6061 agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt
     6121 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     6181 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     6241 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     6301 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     6361 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     6421 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     6481 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     6541 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     6601 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     6661 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     6721 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     6781 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     6841 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     6901 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     6961 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     7021 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     7081 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     7141 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     7201 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     7261 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     7321 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     7381 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     7441 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     7501 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     7561 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     7621 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     7681 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     7741 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     7801 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     7861 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     7921 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     7981 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     8041 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     8101 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     8161 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     8221 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     8281 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     8341 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     8401 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     8461 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     8521 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     8581 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     8641 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     8701 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     8761 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     8821 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     8881 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     8941 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     9001 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     9061 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     9121 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     9181 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     9241 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     9301 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     9361 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     9421 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     9481 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     9541 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     9601 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     9661 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     9721 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     9781 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     9841 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     9901 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     9961 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
    10021 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
    10081 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
    10141 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
    10201 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
    10261 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
    10321 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
    10381 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
    10441 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
    10501 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
    10561 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
    10621 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
    10681 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
    10741 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
    10801 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
    10861 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
    10921 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
    10981 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
    11041 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
    11101 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
    11161 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
    11221 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
    11281 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
    11341 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
    11401 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
    11461 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
    11521 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
    11581 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
    11641 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
    11701 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
    11761 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
    11821 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
    11881 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
    11941 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
    12001 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
    12061 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
    12121 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
    12181 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
    12241 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
    12301 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
    12361 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
    12421 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
    12481 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
    12541 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
    12601 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
    12661 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
    12721 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
    12781 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
    12841 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
    12901 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
    12961 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
    13021 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
    13081 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
    13141 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
    13201 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
    13261 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
    13321 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
    13381 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
    13441 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
    13501 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
    13561 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
    13621 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
    13681 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
    13741 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
    13801 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
    13861 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
    13921 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
    13981 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
    14041 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
    14101 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
    14161 gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg aaatgctgta
    14221 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
    14281 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
    14341 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
    14401 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
    14461 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
    14521 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    14581 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    14641 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    14701 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    14761 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    14821 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    14881 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    14941 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    15001 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    15061 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    15121 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    15181 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    15241 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    15301 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    15361 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    15421 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    15481 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    15541 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    15601 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    15661 aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    15721 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    15781 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    15841 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    15901 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    15961 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    16021 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    16081 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    16141 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    16201 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    16261 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    16321 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    16381 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    16441 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    16501 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    16561 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    16621 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    16681 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    16741 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    16801 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    16861 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    16921 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    16981 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    17041 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    17101 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    17161 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    17221 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    17281 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    17341 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    17401 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
    17461 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    17521 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    17581 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    17641 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    17701 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    17761 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    17821 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    17881 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    17941 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    18001 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    18061 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    18121 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    18181 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    18241 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    18301 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    18361 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    18421 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    18481 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    18541 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    18601 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    18661 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    18721 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    18781 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    18841 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    18901 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    18961 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    19021 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    19081 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    19141 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    19201 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    19261 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    19321 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    19381 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    19441 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    19501 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    19561 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    19621 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    19681 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    19741 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    19801 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    19861 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    19921 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    19981 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    20041 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    20101 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    20161 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    20221 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    20281 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    20341 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    20401 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    20461 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    20521 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    20581 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    20641 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    20701 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    20761 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    20821 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    20881 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    20941 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    21001 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    21061 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    21121 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    21181 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    21241 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    21301 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    21361 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    21421 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    21481 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    21541 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    21601 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
    21661 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
    21721 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
    21781 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
    21841 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
    21901 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
    21961 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
    22021 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
    22081 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
    22141 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
    22201 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
    22261 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
    22321 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
    22381 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt
    22441 tgtacgaaaa aatt
//
LOCUS       DAZUKT010000050        21807 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000050 DAZUKT010000000
VERSION     DAZUKT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.050"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..830)
                     /locus_tag="ACG816_001809"
     CDS             complement(204..830)
                     /locus_tag="ACG816_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920685.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1125..1460)
                     /locus_tag="ACG816_001810"
     CDS             complement(1125..1460)
                     /locus_tag="ACG816_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4920686.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1447..1731)
                     /locus_tag="ACG816_001811"
     CDS             complement(1447..1731)
                     /locus_tag="ACG816_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4920687.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1787..3310)
                     /locus_tag="ACG816_001812"
     CDS             complement(1787..3310)
                     /locus_tag="ACG816_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4920688.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3303..4478)
                     /locus_tag="ACG816_001813"
     CDS             complement(3303..4478)
                     /locus_tag="ACG816_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4920689.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4482..4667)
                     /locus_tag="ACG816_001814"
     CDS             complement(4482..4667)
                     /locus_tag="ACG816_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920690.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4633..6327)
                     /locus_tag="ACG816_001815"
     CDS             complement(4633..6327)
                     /locus_tag="ACG816_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4920691.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6324..6797)
                     /locus_tag="ACG816_001816"
     CDS             complement(6324..6797)
                     /locus_tag="ACG816_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS4920692.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6866..7021)
                     /locus_tag="ACG816_001817"
     CDS             complement(6866..7021)
                     /locus_tag="ACG816_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS4920693.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7155..7535)
                     /locus_tag="ACG816_001818"
     CDS             complement(7155..7535)
                     /locus_tag="ACG816_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4920694.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7539..7754)
                     /locus_tag="ACG816_001819"
     CDS             complement(7539..7754)
                     /locus_tag="ACG816_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920695.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7757..8164)
                     /locus_tag="ACG816_001820"
     CDS             complement(7757..8164)
                     /locus_tag="ACG816_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS4920696.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8430..9905)
                     /locus_tag="ACG816_001821"
     CDS             complement(8430..9905)
                     /locus_tag="ACG816_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS4920697.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9895..10743)
                     /locus_tag="ACG816_001822"
     CDS             complement(9895..10743)
                     /locus_tag="ACG816_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS4920698.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10780..11142)
                     /locus_tag="ACG816_001823"
     CDS             complement(10780..11142)
                     /locus_tag="ACG816_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920699.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11143..11301)
                     /locus_tag="ACG816_001824"
     CDS             complement(11143..11301)
                     /locus_tag="ACG816_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920700.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11442..12066)
                     /locus_tag="ACG816_001825"
                     /pseudo
     CDS             complement(11442..12066)
                     /locus_tag="ACG816_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12063..12374)
                     /locus_tag="ACG816_001826"
     CDS             complement(12063..12374)
                     /locus_tag="ACG816_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920701.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12418..12711)
                     /locus_tag="ACG816_001827"
     CDS             complement(12418..12711)
                     /locus_tag="ACG816_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920702.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12905..13552
                     /locus_tag="ACG816_001828"
     CDS             12905..13552
                     /locus_tag="ACG816_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4920703.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13613..14758
                     /locus_tag="ACG816_001829"
     CDS             13613..14758
                     /locus_tag="ACG816_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4920704.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14839..15144)
                     /locus_tag="ACG816_001830"
     CDS             complement(14839..15144)
                     /locus_tag="ACG816_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4920705.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15169..15600)
                     /gene="ruvX"
                     /locus_tag="ACG816_001831"
     CDS             complement(15169..15600)
                     /gene="ruvX"
                     /locus_tag="ACG816_001831"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4920706.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15597..15863)
                     /locus_tag="ACG816_001832"
     CDS             complement(15597..15863)
                     /locus_tag="ACG816_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4920707.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16118..16843)
                     /locus_tag="ACG816_001833"
     CDS             complement(16118..16843)
                     /locus_tag="ACG816_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4920708.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17204..17274)
                     /locus_tag="ACG816_001834"
     tRNA            complement(17204..17274)
                     /locus_tag="ACG816_001834"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17240..17242),aa:Gly,seq:ccc)
     gene            17391..18143
                     /locus_tag="ACG816_001835"
     CDS             17391..18143
                     /locus_tag="ACG816_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4920709.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18159..18638
                     /locus_tag="ACG816_001836"
     CDS             18159..18638
                     /locus_tag="ACG816_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4920710.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18889..20469)
                     /locus_tag="ACG816_001837"
     CDS             complement(18889..20469)
                     /locus_tag="ACG816_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4920711.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20462..21205)
                     /locus_tag="ACG816_001838"
     CDS             complement(20462..21205)
                     /locus_tag="ACG816_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920712.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21381..>21807)
                     /locus_tag="ACG816_001839"
     CDS             complement(21381..>21807)
                     /locus_tag="ACG816_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HFS4920713.1"
                     /translation="YEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHYD
                     EILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITS
                     ISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         6369 a         4419 c         3538 g         7481 t
ORIGIN      
        1 ttttatatct tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgagaa
       61 acccatatca aagaactata aaggttatca aaacagcgtt tatgactaaa tgaattagag
      121 ttatctatca ctatttttca agcgatttaa accaaatggc atacaatatg gcatacaaaa
      181 acttcaataa gccgtggggg tttttaaatt tcttcttgtt ctttaaaatc aatgagtgga
      241 gctacataat ttgaatccac tttttcaaac gtcacttttt gcatcgacga atgttttctt
      301 tcccaagata acgtaaatag atttgactct ggacttataa agtctacttc accttctagt
      361 attactttct tatgcaaata ttcctctaat aaatatagac ttgctaaagc atacaacaca
      421 ttttccaaat ttgcttcctt ataatttgaa gaacgaccat gttttactct gttatatatt
      481 ttccaccaat ctggtgtatc ttcaggccac ccatctccaa aaggttttag tgaagtcaat
      541 gttaaagaat tcataaccct aacaccatta tccaaacaca ttaattcgct agatttaata
      601 acagctttaa aatcagttat gcttttattg ctactgctat caaatccgca aagttctttg
      661 aaaataatct cacactccga accaatcgct agtagtagtt tttgatattc aatagagaat
      721 gttttgaaat tattctcatc gagcatcaca aaatctgtgg tattaataaa ctcattttct
      781 aaatgtagat aatacttcca ataaaaattt gtaaactctg ctttgtccat taaatcacct
      841 cttttgaatt ataacaaaaa aaacacgtcc acaacagacg tgttctttga tttctacaca
      901 atcagcttgc caaaaccatc caatcagtcc tagcatgcct gtattaaaat tgaacgttca
      961 tcatctatca tctttcttag tccagcatgt ttgcaaccac acctacaatt ttcagtccaa
     1021 caatagattt cgactagcta agtcttgcaa aattcaggca gctaaccctt catttactac
     1081 cttaattata ctaaataccc acaacgttta caagtgtaat cattctaaga aatctgttta
     1141 gctatgatcg ttgtataatc tttaaacgcc gttccttcat tgattgaaat aattttataa
     1201 ctcttgtttt tccaagctac tttcatatcg tttgttagtt cataatcttg atcgtatcga
     1261 ataataaacg tcaacgttcc ttcaagaatg gtacctacac tagcaagcac gtcttttagc
     1321 atttgtgaac gcacattagc ccaacattca aatacagttt cttcactatc caccatctgc
     1381 ccatcttcat cttttacccg ttttgtcttt ttaaacttga ctctctcagt caattcattt
     1441 acattcttaa tgattggcat aataagcacc tctcatttgt tgtactaatg cttgtaccgt
     1501 tactggtatc cgttcgctgc tggcttcttg acggttcaaa taccaatgtt gagtaagtaa
     1561 tgacaccgcc aaatcaaatt gctgataatt atccatatct ttatccgttg cgctactatc
     1621 aatagcactt tttatatatt cgcctgctgt atcaatcaat tgttgaataa agtcatcatc
     1681 aatagtgtga tcgatccgca tacttttttt tatgctatcc aaaattacca ttttatctct
     1741 cctaatgaaa aaaaggcagt gggataaacc gccgcccaat atttttctat acttttggtg
     1801 ttacaactgg cgtaaactca atatacactg aagcattttg atcaattttc ttataatcgt
     1861 tgcgaacgat cactgcaagt ccttgtgagt agtaatcgaa tttttcccat tgagtcgtta
     1921 cttgatttct acgagcaaca aagatagatt gtgcaatatc ccccataatc atagggaatg
     1981 ttccagcttt aggattggca aacaatgtat ctgcaatcaa tactactggc ataccgaata
     2041 atgatttacc actaggtgct gtcacatcgg gttgtaaaat gtaacgtcca tctgaatctt
     2101 tcaatgtatc taggtggtaa tatccgcttt ggtttagaat taccatttta tttaatgctg
     2161 ggtctaatgc cacattgtat agttgtttca aatcgtccaa cgtagcagcc gttttcttag
     2221 tgaatgtttt taataaatcc atgatatgct tattgtcagt gttttctacc aatttcgcta
     2281 actgatcttt gacctcttgt acaatattca ctgctgaatc ttccacaacc tcatttgata
     2341 aggcaatctt accagcacga gtttctactt tataatcaac tgaagtaaac atttcagcgt
     2401 caatatcacc aatttcagca agttcagctt ttgttgctaa cactgcttgt tggttagttg
     2461 ctactggata tttaccttgt ccatttgata ctgtttttac tgttgcatat tgagctaagt
     2521 tataatttga acgtttcaaa tcaaagactt caccgattac ttcttcggga acaactgccg
     2581 ctgcatttac agtagtgact ccatcacgca cttcgccttg tgaacggata tattcttcat
     2641 acccacgcaa ttctgttttt tcgttatcga ttaaagtttt ttccatgtta ttactctcct
     2701 tttgtgcttt agaagtggat tctaagctat tgtttgtttg ttttgcttgg ttgttactca
     2761 taaacgattc gtaagaacgc ttatttactt ggacatttga tgaatcataa gcgggaacgg
     2821 tcacgacact aatttcatta agtgctttga ttttatctat tgatcgttca attgtgccat
     2881 cttctttttt gtcgaatgaa tctaatccaa gcacgaaccc aaaggacata gaatcaacaa
     2941 ccccttttga gatattctca tacacatcat ttgaataggt cgtgtcggtt agctctgctt
     3001 caaaatgtaa tcctgtatca tcgacattca atttcaacgt acctgctttg acactagcta
     3061 gtggctttga ataatcgtgt ccatataata agaacacatt tgataaatcc acctctttta
     3121 atgcttcggg tgtaatcact tctacaaagc cgcctaaatc ttttgacggt tgcccgaatt
     3181 tcaacgcata gcctgaaata gttttgccgt ctgttggttg tttttcattt tctttctcta
     3241 ctttgggaga atcggctgtt agctcagctt cttctgtcag ccgtttttcc ttttcttctt
     3301 cattattcaa ctggtgtaac tccttcctgt tcttttaatt gatatttctc caacgtatct
     3361 aaatacgtaa agttcaaact tgctagtaaa cgatcgccac catccatggg gggtagcccc
     3421 attttcgacc gcccctcatt aatcgtgaga agtgaccctt tgacctgatc caatacattt
     3481 tttacttttg tttcaggatc agtctctagt aactgatcgg aattaaagcg gatattggtt
     3541 tcaagtttcg tatctaattc actcacaaac acattaaagt aatgaatcag cgtactttgg
     3601 atatactgga gattactttg aacggtgcta gaatgttcat tttcaacgcc taaacgctca
     3661 atcggtacac caaacgcctt ggcaatttgt tttgtgttcc aatcattgga attgataagc
     3721 ttcaaaacat cggtgtttat ttctaatgtt ttgtagtcca ttgtttcatc aagaatgatc
     3781 gttctaagag cattatctcc actacttgaa ccgttggctt cttcaaactt ttcacgaatt
     3841 gcgttctttg ctgatccgtc taaatctgac ttatgaacct tcaaaatacc tgacccactg
     3901 acaccacgag taaagaaatt gtgcaatgtg cggttgccag cttgttgaat tttcagttca
     3961 tcacgtaacg cataaagtgg tggtaatcct gttaaaccat cttgggtgaa atatttgaag
     4021 tgcaaaatat cgctagactt cacacgtctt tttttatcac caatctgata agacaacgta
     4081 ccattatcta attgagtaac cgttacactt gagttaggta ataagtgaat ttcttctacc
     4141 ttttcaccgt tgcgtttaat ctctgcaaag ctattaccgt ttagcaacat attgaccgcc
     4201 aaagcaaact ttaaatgcca accatccatt tctgaattag gtttctcgtt tagtaacttc
     4261 accagttcat tatcagcttg tggcatattg tttttgacca attgaatcgg acttgaagca
     4321 atatcactag caataattcg aacggctgca aacacatcac tatttcttaa tgcacgaacg
     4381 cttgtaaagc tggtggtgta atcatccgat tggattgata ccacatgttg taggaaagga
     4441 tctcccgttg tattttgtct cgctttaaaa aatgccatct tttaacctcc cttctgttct
     4501 gtatttagca tgaccgctac cccaataagc actatgccca gcgctaacaa gccatagtag
     4561 acatttgtta gtaaggtgat agcaaccaac acacacacca atccaagaag taaaagaacg
     4621 gtatgaatgt aattagaaag aaaattcttc acttgtataa aattcattgt ctgctttcgc
     4681 tccttccgtt gtggtgaagt aatccattgc aaacacatag gcattgatta atgcagctat
     4741 tggatcaatt ttgttactgt tctttgattt attaatctga atgccgttgt tatccgtttt
     4801 taaaatagcg ttgttcactg catgggtgag aatcgtattt ttattgtgaa taacgttgcc
     4861 ttcataaact tgttctctaa aagttcgggt cggaacgttt agagtaatcg ttccttgtct
     4921 tacttctaac attgggtagt tggctttttc agctttactg ataagcgaat tagcgttata
     4981 cgggtcataa caaatcccca tacattccaa atcattttct tgaatcaaat cttgaataaa
     5041 ctgaaatact tggtcatagt ccacgattcc actttctaat tgagtaatgg aacactcacc
     5101 agctttttct agttctcgat aaggcaaacc gtcgcgcttt tctttgtctt gaagcccata
     5161 tttggtggct acaaagctat gtgaatcaca ataaagttcg ccgttatcta gcggtacgat
     5221 ccatgaaacg ctagttaaat catctgtttt agataaatcc actccgatat aaacgggctt
     5281 gcctgtaata tcttgtggtt ctacttcggt tgcctgccag tcttcagcaa tcatatagct
     5341 gtcctcactc gcttgtctcc ataaattgaa gtttttcacc aatacagaat taaggttatt
     5401 ttgtttcaaa gcaaggttca catcatcttg aatggttgga atcattactt ttttgatttc
     5461 ttcactttca aaaatcggat tggctttgat ccatttctct tgatcgtgaa tttcttcggg
     5521 atcgtccaat tcccatatcg ctataaagta cctgtcggct tgttcttcgc cttttagcac
     5581 acgatctaac aataagtatt cctcgtacat aggaacgttt aaatcaagcc cagcggtact
     5641 aataacacac aataagccgt tcttttgttg ggtcattcct gacttaatga cgttgtacgt
     5701 tttacgagtt tttgcttcgt gccactcgtc aagaattgcg gttgttggtg caaacccatc
     5761 tagcgtgctg gtttcactag ccagtgccat agcgaacgaa ttagagggca agtgctgaat
     5821 cttagagttc ataattttca gttgtggtct taaaaactta ctagacttga ccacgtttct
     5881 aagtgaatta gacagcatat catagcccaa tttagcttgt ttcaaagcgt tacttacaaa
     5941 caatacttgc cttgcttctg ctggttcttg ttccataatc aacgcattgg cagccatgcc
     6001 acttgcgata tacgttttcc cattcttacg gctcatactg gtaaacgctc gattgaatcg
     6061 cctgtaattc ccttctttag tacgccagcc ataaagactg cctagaatga atttttgaaa
     6121 tcctaacatc ttgattgctt taccgtccgt agatggtagc aactcaatga atttaatcgc
     6181 tttggtggct tgttcttcat cgaacacata agggaagcta tctgacttag aacgctccaa
     6241 atcgcttata tgacgctcac acgcttggat aattttcttg ggtgcaatca gcttacctga
     6301 taggactttc tcaatgtaat cattcatgaa ttcatcaact ctttgaacgg gtcttcgggt
     6361 tcatcatttt taaccttgtt caaagctagt tttgatcgtg attctaaggt catacctagt
     6421 gaggtggctt gttttttcaa atcactcata gcttgtgatt gaacccgaac cgctggattg
     6481 gctttcttta ctccgttttc gcatgtgata aacgtacctg attttcttat ctcggcttcg
     6541 gctgttttca ttcgagcaaa ggctaaacaa taggctacca atgaaccata atctgcttca
     6601 ctcaatggat aatctttttt taatgttggt acgatacgat tccattcact tctagcgctc
     6661 gctgccatcc agtcaggggg cgtgatattt attaatggtt gctgcttgaa caattcttct
     6721 ctagcgtcca cacggattgc tttttcttca cttgatatat tgccttttgt atcgtgtaat
     6781 attttagggt gtcccatgtt gtcctccttt cagacaaaag aaaaaggaac gtaagcaatt
     6841 aagctcacat tccttcgttt ttcgatcaga atttttcttt atctaattta tctatataaa
     6901 tgatttttcc gttctgcatt tgaatggtca cttgaccaaa atgaggtaac tcaatggtgc
     6961 gctgtttccc tgttttatca gtaataacaa ttgtattttt caataaatct cctactttca
     7021 ttatatactt tttcacttgt gattacaagt gtaatcgtta tttgtaatta cccttatgcc
     7081 ttttcacaac ggttatttgt accaaaatga gtaggcatcg attgtcagta agtttcacac
     7141 tatgccccct taactcaaag ggtactcttt tctcgtcttt gtctttcatt ggctgtcttg
     7201 gaattatggt gtgcatggca taaagaacga aaattagttt catctaaccg tctattccaa
     7261 tcatctttta gttcaatttt gtgatctaca acatcagctt tccttatcaa gccttcttct
     7321 aggcaatcct cacatactgg atgatttaac cgatacaagt atgaagcctt tcgccatgtt
     7381 ctactttgat agaattgaaa atatttacct tcgctttgct ttctttcttc acgcttaatt
     7441 gccgtttgct ttgctttatg cttcccacaa tacttctgcc tataatcaat caatgtttta
     7501 catccagcat gattacattg ttttttaact gccatagatt accaaccaaa gcgatctcgt
     7561 tcgtcagaat catcttgttc ccattttgat ttgtattcaa tataaggcga aactatcttc
     7621 aagcacgttc cgtctttaaa attaacttta gtgattttat cttcctcaaa actaataaag
     7681 tctacatctt cttgagtgtt tgaatactca atgtgtttct caccatcgaa gtatctaata
     7741 ctttccatat gcataattat tctacctcca ataaattagt tatcaattca tcatctgtta
     7801 gtagcaaagc caagaactta tcatggatgt ttaaaaacaa attatcctca ttcgatgtaa
     7861 gctctcgttg tctcgctcga tcatataggt taagcagaca attaaattca tccattagct
     7921 cacctgtatc atcatagttg ccactatcta ccaatgatcc cactactagc gattgacacc
     7981 acgctactga ctcctgcagc caatcaggtt cttcttccat tatctttaat acttctggat
     8041 caaatgtgga ataatcttct gttaccaatc ccacactaaa tgccagcgtg ttcattagaa
     8101 accgatccat ttcatctgta tattgaaagt tcggtgcttt gtttgttgaa ataatattga
     8161 gcatttactt tcctccttga ttcgtaacac gtggtaacac ctacgtaaca ggtacccgtt
     8221 accactcaat cccaacgggg acaagggttc tagcgttttg gtaacaggta acgggtaaaa
     8281 tccagtaaca ttattcccca gcatattagc taactatttc aaaagtttta aggaaataag
     8341 gtgttacccc gttaccaaat tgctgtatcc cttggggcag taagggtaaa ctagtaacgg
     8401 gctaggtgtt actgacccgt tactacccgt tactttttgt actttccagc tcgattttta
     8461 ttttcatagg catttgactt attgtaaaat cctcgtcgtt tatttctttt tggcagttgc
     8521 ttccgttccc aaccaggaat actgctaata atcaaggcaa tctttttagt ttctgcatct
     8581 gatcgaccat tcaataaatc ctttgcttgt ttatcaaaaa cagcttccaa aatatcagcg
     8641 gtgagtacgc tatcaactaa ataaaactct tgttgcatag tagctccatt atagaaatgc
     8701 tgtaaatttt tctcattaca tttactgtca ggatagttct gataataaga acgtctcgcc
     8761 cacgctggag cttcttccca tttatacggg atttccattt ccaaatattt agtgattgct
     8821 tctttaattg gattctccat cttggcatcc tcttgatagt cttttgcaat ttctaagact
     8881 tctttatctt catcgggact aaaatagatt cgttgtcctt tatcaaaaag cacctttgct
     8941 tcagctaaca cttgaagaaa atattcatcc tttgttttaa acggattcat ctttggctta
     9001 ttcttacaag gcaaaggata aaaacggcgg tttcctgtat catctttcaa atactgccca
     9061 tcattcgacg ttccaataaa tacacattgc cttgcccaag ctatgacatt ccgaccataa
     9121 ggaggtctaa ttttatcgat cttggcactg atgaagtttt ttatctgttc aattttagtt
     9181 ttgttcattg aagataattc tccaagttca ataattacat tgccttgaag ctgcatataa
     9241 tcatctttct tttcacctag cgaatctaat gaatcggtga aaaaatcatc cgtgtaaagt
     9301 aatgctggaa cggtactctt accaatccct tgcggtccat ccaaaactgg aacgatctca
     9361 aactttattc ctggtttata gatacgtgcg actgatccga ctatccaacg ttttgttact
     9421 tctcgtgtgt aaaggttgtc gtctacccct aaaaggtcaa taaacagtgt ttccagtcta
     9481 ggctgttgat cccatttaac tttttcgata cgttctttta ctgggttgaa aacattcttt
     9541 ctagccacat ttactactgc tttgtgtatt ttgttgtcat caaaaactac attgtagttt
     9601 tcttcaatat atgccgttaa ggcatcctca aaactgtctt cccattctcc agcggtacga
     9661 ttgatccaag gagaatcttt taataggtga atgtatccgc taaattcgtt ataagcaatt
     9721 gttccagcta aattgctatc attggttaaa atcaattctg cattcacaac actgttactt
     9781 ttaatctgat cgtctttatt gcatcttaat cgtgacatcc aggttttcaa ttcaatgata
     9841 ttgtcttcac gctcgctggc ttcgggaaag tctaaaatac tatctaattt atgcttatga
     9901 gccatgagct ttccccctca aatctttttt aatcatcgat cgaataattt gattcacttc
     9961 tttatctttt agcggtggtt cattgtacgc atttgctgta taaatcaatt ctgctaaagt
    10021 ttgattatct acaccgtgcc atagtaacca accgcataac ttagttaaat gatcgtttct
    10081 actaccttcg ccagcgcctt gaacaatcat atctaatttt tttgctgtcc ctgtttttgc
    10141 tacgggtcgg tattgagtct ttatttcttg cggttttttt agcagtaggt tcactagcca
    10201 atcgggagct ggtgcaatat tatttagaga tacgttttca ataggttcat atgctctccc
    10261 aacacttctt gaaataatca tagttggcgc tattggaact cctttagtga taatatcaat
    10321 cccgcttaaa cttttacctt tatcatcacg cccgaagata tcagcaaatg cacctgtttc
    10381 ttgttggata attacaccat caggaatttt aaagaagaaa tggattcctt tacctggtgt
    10441 gatttccgta taagtagaag gaaatggatc atacattgaa gcaagttttt tataattctc
    10501 aacgccgttt cgtgtttcag aatgttgatc taggtcaact accaccattt gatgatcttc
    10561 aagcattaac ccaatatttc tagttgcatc ttccgtccac cattcattaa tttgattaga
    10621 atcgttagtt gcatcattcc aaccactaga gccttttatt ggaattttag ttccaggaga
    10681 taagggataa attgaccatc ccgacttttg taattccgtt gctgcttgat aattatttgc
    10741 caataaacct ctcctccttt ttcaaaatac tggtataaat cattcagaca cctccatgag
    10801 cttcaactgc tcaagttcgt atttcaaccc atcccgaaca aattcagcac cttttaattg
    10861 attctgatac tttacccatg tattaaatgt tttcaccctt gcttcttgca tatcagtaat
    10921 gtttaaccat gcggttaaaa tctgattttt tagctcgttg atttctcgac aaattatttt
    10981 gtgttctgca tgttttttga gtacatacaa aactgttagt ttttcattga atgaatttcc
    11041 aaagtaggtt ttcccgatat tattttctag ttcagcgatc ccttgattaa tttcctctaa
    11101 ttcaaggcta tcgagttggt ttaatagttt tgttttgtac atctacttat gccctccagt
    11161 tcttaattga tagtcgtact cgtcataagc agtgaagaga tataacgcta atcctgcaaa
    11221 gatagctatt tttgcaatta atggtaataa gccgaaaatt ccgccgatta aaatgcttcc
    11281 tataattaag tttcgtttca taatggttcc ctccgatttt ttaatatggt ataatcggaa
    11341 caaagaatat cgggttttat cccaattaag ttgcctttat cctgtccgcc aagactgtta
    11401 tggataaggc tttttctttg ttctcgatca tgttaatgct cctaagttgt ctgcaattca
    11461 gcagaacgat tataaatttt ctcactcact gaaaactgca acccatattt ttctttaaca
    11521 gtcattaatt ctactgtctc atctaaaata ggttcacgat caaccaacat ttcaggagtc
    11581 atatctttct tttttaccat tttggaatga ccataaatag ttgatacagc tttattggct
    11641 atcacattcg ctttaatgta gtctactggt tttggttcag taatagcgtg gcttaattta
    11701 gtcattgctt ctttttggtg ttctttgtct agcatgcgga atacttgaaa gccttcaagt
    11761 cctgtggact ggcgtagttc tttgatgatg tcgaaaatcc agtcttgaaa tgcctcggct
    11821 tcttttttac ggcttttgaa tgccatgcgg taaatatttt ttttcgttga tgatgtttac
    11881 atcttgtgtt ctgcccaatg aatcgatgac cttactagta gtaaccccat ctttaggcaa
    11941 agagtgaatg gctctagtaa cctgtttaag acctaacgct tccgcaacat ctttcgccac
    12001 tgcccaccat tcatcattaa tatcgacaaa tcttataatg tgtccgttcc aaatttcagt
    12061 tttcattcaa cgtcctccag ttcaagccct agcaagctcg ctaacaactc aacttgctga
    12121 tacaaaattt cttgatactc ttcaatagaa agtcgttctt taaactctgc ttgtactaaa
    12181 tcgttcaagc cacgcaatac tccgcttacc gttggttctt gtggtagcaa tttttgatag
    12241 tccatttgtt cgtacaaaca ttcaaacgtt tcttctagcg ctttgccttc caagtttgct
    12301 aattctgccg ctgtgtcttc tgtaagaatt gttgataata ctttttcgat tgctttattt
    12361 tgtgtcgatt ccattattat ttcctcctat taatcccttt gcccccacgg cttgcgttta
    12421 tttttggtat tcttccataa agcgatcaca ttcatcttgt gaaattctta cgactccatc
    12481 aactataatc aactttaaac cgttaggaac atatttctgc atcatagtat tgtatgaaac
    12541 gttcatatac ttagcagctt gtgccttgtt catatatttt ggaaactggt actttttttc
    12601 taataatgaa agtaacgttc cttgagtttg ctctacttgt tgaacaactc gatcgtcaat
    12661 aattttaaaa gctggatcat caagcgtgag tttgtcaatt tttaattcca tatttatccc
    12721 ccctcgttag catacatttc tatacaaaga ttttaaaaaa atgcaagagg ctcttgaata
    12781 catttctata cctcttataa actcaatata ccatcatacc ttttcatttg caagaatttt
    12841 aacatacttt tttgaacttt tatgtttcac acatatttaa atcgtgatat agtagagaaa
    12901 atatatgaat ggtggtgaaa atgtggctag aaaaaaatac cccgaagcag tggagacagg
    12961 acaaagaatt agactggcta gaattgaaaa gggactctca atggaacaac ttggaggtaa
    13021 attatcccca ccggccagta aaggtgccgt tagtaattgg gaaaatggct ataatcttcc
    13081 caataacacc aggcttaaac aactttcaaa ggtattagat gtatccacta cctatctatt
    13141 aaacggatct tatacattaa gagatatgca tatgatgccc gaaattaagc gaaatgtgct
    13201 tgaaaagcaa ttgaaaaaaa ctattgaaaa aaataactct ataattgatt ctaatctttc
    13261 taatgctatc aataacatcg aagaagataa cctgactgtc tcacagaaga tttatttaac
    13321 aaacgcaata aaatatctta aaacaggatc tcgaattgaa tttcctgacg ggagtacccc
    13381 aattgcttat ctcgcagctc ttctaaatac gcttttacga tatgactcat taattaatga
    13441 ttcagaaaaa aatacagatg agaaaaaaga gtatcaagaa ttttatgaaa atgatcttct
    13501 aaaagagttc acagctcttg ttactgagct aaataaacac tattcaaaat aataataaac
    13561 tgcctcctcc aaccgccccc acggcttgct acactatgga gggaaaacta ttatggcaac
    13621 attcaagcaa tatgaaacta aaaaaggtaa attttggctc tatgaagcct atcttggaat
    13681 gaacaaaatg actggcaaac ctgataaggt tcgtagacga ggttacaaaa caaaaaaaga
    13741 agcccagctt gccttgtcac gcttgcaagt ggactacgat aaaaacggta ttaaaaaagc
    13801 gaacaacgaa acgtttaaag aagtttatga cttgtggcta gaaacatata aaaccactgt
    13861 ccgagaagcc accttcatga aaactgaaat caagtttaga aagtggattc ttccaaaata
    13921 tggaaatctt cgtgtgaatg aagtcacggt aaaaaaagcc caacaaattg taaatagctg
    13981 ggctgaaact actgaccaat acaaggtact tcattcaact tctaagcgta tttttagcta
    14041 cgctatcaat cttgggatta ttgagactaa tccactagac aacgtattaa tgcctaaaag
    14101 aaccaccact aagcataaag aaaaggtcaa agtttacagt aaagaacaac tgatcacctt
    14161 gtttgattac ctaaacagca aacctgaaac ttatcgaaac gactttgata aaacgttact
    14221 acgctttctc ttttatagcg gggttaggat cagtgaagcc cttgcattga attggtcaga
    14281 cattgatttt caagaaaata cggtcaccat caataaaaca ctaagccaat ccaaaaatgg
    14341 ctataaaatc agtgatccta aaactgattg tagtgctggg gttcttccac tagatgaaag
    14401 aacaatttct atattaaaaa aatggcagat taatcagcgg aaatatatgc tgacacttgg
    14461 tgtaactgat ccgactatga ttttttgtgg tatctataaa gaactaatca cacaccacgc
    14521 tatttatgca agattgatta ctatttgtga aaaagctatg gttccatttc tcggtgtgca
    14581 tgtagcaaga cacactcatg cttcaatgct cttggacgct ggagctacta tggtagaagt
    14641 tcaaactaga ttacggcata caaaaattag tcagacgatc gattgttacg gtcacttggc
    14701 aaaagaaacg aaagaaaaga ctgttgaaaa attagtacaa cacttaaata ttgactagat
    14761 ggcatacaat atggcataca gtaaaaaata aaaaaaggag aaatcctatc atatcaaggt
    14821 ttctcctagc tttatagtct attcttcttc ttccgccatg aatgtattga atacttcttc
    14881 aatcatgtcc cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc
    14941 gttttcgatg tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg
    15001 atataaaaga acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc
    15061 atataatgtt tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc
    15121 gtgggaatgg ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg
    15181 cttacctgag ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac
    15241 tttttttcgt ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac
    15301 agtggttaat cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc
    15361 tccgtatgac atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa
    15421 tccaactaca aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc
    15481 aaactcttct tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa
    15541 ggggtcactg atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat
    15601 aaattcactc catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt
    15661 tcgtgacggc gaatcaaatt tcgagcatca cgataacgag gaatgtagga cgggtctcca
    15721 gaaagcaagt agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga
    15781 taaacaatcg ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt
    15841 acggtttcat ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt
    15901 accttctaca accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact
    15961 tccgaataaa ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca
    16021 aaagtactct tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt
    16081 ttttcataat attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt
    16141 gtcctgtctc ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt
    16201 caattttttt gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat
    16261 tcccctcaaa atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg
    16321 ccgcaatatc attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca
    16381 tcaagttttt aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat
    16441 tatataacca ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca
    16501 aaggctctct cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta
    16561 ctctgttcaa tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat
    16621 cctcttttgt aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat
    16681 attctggcac atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct
    16741 gctgaaagat atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag
    16801 cagcgacgat ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact
    16861 aacagctccc taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa
    16921 acagcaaaaa ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc
    16981 tcgtctctaa tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct
    17041 tcatactcaa gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg
    17101 catctgcggt aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt
    17161 tcttattact tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc
    17221 gaacccgcga catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca
    17281 aaaacagtat acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt
    17341 atgttttgta gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa
    17401 aagaattaga tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca
    17461 atgcggagat gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt
    17521 tccgtttggt cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga
    17581 ccatccgaat ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa
    17641 atgatctttc acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac
    17701 tgcaagcaat cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg
    17761 cagtgatcag tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga
    17821 ctttagtcat tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa
    17881 tcaaattttt atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg
    17941 tacgttttgg cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag
    18001 ttcctggagt tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag
    18061 gagtttctcg cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt
    18121 ttattttcca gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt
    18181 tttcttttag ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt
    18241 cattgatttg ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc
    18301 aaataggcgg atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca
    18361 gcgatctttt ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg
    18421 ttcctctagt ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct
    18481 atcgagaagc ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag
    18541 gtcttgtatt atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg
    18601 caatcatgat gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg
    18661 ttgtgaaaga gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag
    18721 taaccgcagg agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct
    18781 tctcacattt ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta
    18841 tctgtaaaaa aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt
    18901 tttccaaaac gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc
    18961 gatcacagga atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac
    19021 gataagaata atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc
    19081 tgaacctctg ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc
    19141 tctagcgaaa taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct
    19201 agggataaaa aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt
    19261 taaaaaactc tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga
    19321 gattggcaaa gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat
    19381 aaaagaagtc tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc
    19441 gattaactta ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat
    19501 aaaaatgatt ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt
    19561 tactaattga gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt
    19621 tctttttatt ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat
    19681 cagctgtttt acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa
    19741 taaaccttgt tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat
    19801 cggatgccga tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat
    19861 tccagcaaat gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg
    19921 ttttttaaac actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag
    19981 aataatcaaa aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag
    20041 atcagctcgc catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat
    20101 ggtcaaagca actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg
    20161 caaaaatact tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg
    20221 agttaaacct aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac
    20281 aaagttcata gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag
    20341 attttgagac aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt
    20401 attcgtgagc tgtggattaa catataaaag attaactcga attaattcta ataattgtcg
    20461 cttatacata cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt
    20521 ctagcgtttc atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt
    20581 cacctttctt caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg
    20641 tagaaaaaat gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa
    20701 atgctgcttg aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg
    20761 gcagcaacgt gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg
    20821 tttcattttg atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt
    20881 ttttttgtgt gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca
    20941 attgtaaaaa aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc
    21001 gattttccgt caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac
    21061 taactaaact tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa
    21121 tttccccttg tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt
    21181 tacttacatt gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt
    21241 gtacccttcg ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa
    21301 acgactaggc tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa
    21361 gatatgagtc tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc
    21421 tttagtttat caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact
    21481 atttcgttta ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa
    21541 tctttacata aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg
    21601 agaatctctt catctttcaa tactaggcct attttatttt tataacctgg catgctcatt
    21661 agataaagga tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc
    21721 cacctttttt tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt
    21781 tggatgcttt ttttattttt ttcataa
//
LOCUS       DAZUKT010000051        20395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000051 DAZUKT010000000
VERSION     DAZUKT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.052"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG816_001840"
     CDS             complement(<1..1835)
                     /locus_tag="ACG816_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4920714.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG816_001841"
     CDS             complement(1832..2536)
                     /locus_tag="ACG816_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4920715.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG816_001842"
     CDS             complement(2548..4857)
                     /locus_tag="ACG816_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4920716.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG816_001843"
     CDS             complement(5052..5516)
                     /locus_tag="ACG816_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920717.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG816_001844"
     CDS             complement(5516..6142)
                     /locus_tag="ACG816_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4920718.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG816_001845"
     CDS             complement(6149..6514)
                     /locus_tag="ACG816_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4920719.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG816_001846"
     CDS             complement(6504..6842)
                     /locus_tag="ACG816_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920720.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG816_001847"
     CDS             complement(6832..7158)
                     /locus_tag="ACG816_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4920721.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACG816_001848"
     CDS             complement(7139..7417)
                     /locus_tag="ACG816_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4920722.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACG816_001849"
     CDS             complement(7419..8783)
                     /locus_tag="ACG816_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4920723.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG816_001850"
     CDS             complement(8796..9365)
                     /locus_tag="ACG816_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFS4920724.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG816_001851"
     CDS             complement(9337..10524)
                     /locus_tag="ACG816_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4920725.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG816_001852"
     CDS             complement(10588..12315)
                     /locus_tag="ACG816_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4920726.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG816_001853"
     CDS             complement(12312..12764)
                     /locus_tag="ACG816_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4920727.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG816_001854"
     CDS             complement(12876..13256)
                     /locus_tag="ACG816_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4920728.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG816_001855"
     CDS             complement(13253..13639)
                     /locus_tag="ACG816_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFS4920729.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG816_001856"
     CDS             complement(13641..13919)
                     /locus_tag="ACG816_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920730.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG816_001857"
     CDS             13967..14695
                     /locus_tag="ACG816_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFS4920731.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG816_001858"
     CDS             complement(14841..15317)
                     /locus_tag="ACG816_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4920732.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG816_001859"
     CDS             complement(15277..15633)
                     /locus_tag="ACG816_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920733.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG816_001860"
     CDS             complement(15611..15796)
                     /locus_tag="ACG816_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920734.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG816_001861"
     CDS             complement(15797..16069)
                     /locus_tag="ACG816_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFS4920735.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG816_001862"
     CDS             complement(16066..16266)
                     /locus_tag="ACG816_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920736.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG816_001863"
     CDS             16394..16606
                     /locus_tag="ACG816_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920737.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG816_001864"
     CDS             complement(16639..16833)
                     /locus_tag="ACG816_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920738.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG816_001865"
     CDS             complement(16846..17028)
                     /locus_tag="ACG816_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920739.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG816_001866"
     CDS             complement(17025..17165)
                     /locus_tag="ACG816_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920740.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG816_001867"
     CDS             complement(17655..18020)
                     /locus_tag="ACG816_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920741.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG816_001868"
     CDS             complement(18060..18362)
                     /locus_tag="ACG816_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4920742.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG816_001869"
     CDS             complement(18362..18661)
                     /locus_tag="ACG816_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920743.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG816_001870"
     CDS             complement(18654..18818)
                     /locus_tag="ACG816_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920744.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG816_001871"
     CDS             complement(18824..19093)
                     /locus_tag="ACG816_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920745.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG816_001872"
     CDS             complement(19105..19854)
                     /locus_tag="ACG816_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920746.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..>20395)
                     /locus_tag="ACG816_001873"
     CDS             complement(19851..>20395)
                     /locus_tag="ACG816_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4920747.1"
                     /translation="FIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGV
                     TGRKISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKR
                     VCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQ
                     IKGIKLNQKTIKKLNIGGEK"
BASE COUNT         5656 a         4315 c         3180 g         7244 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaac
//
LOCUS       DAZUKT010000052        20288 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000052 DAZUKT010000000
VERSION     DAZUKT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.053"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1648)
                     /locus_tag="ACG816_001874"
     CDS             complement(89..1648)
                     /locus_tag="ACG816_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4920748.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1803..4037
                     /gene="pcrA"
                     /locus_tag="ACG816_001875"
     CDS             1803..4037
                     /gene="pcrA"
                     /locus_tag="ACG816_001875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4920749.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4054..6090
                     /gene="ligA"
                     /locus_tag="ACG816_001876"
     CDS             4054..6090
                     /gene="ligA"
                     /locus_tag="ACG816_001876"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4920750.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6196..6501
                     /gene="gatC"
                     /locus_tag="ACG816_001877"
     CDS             6196..6501
                     /gene="gatC"
                     /locus_tag="ACG816_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4920751.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6501..7973
                     /gene="gatA"
                     /locus_tag="ACG816_001878"
     CDS             6501..7973
                     /gene="gatA"
                     /locus_tag="ACG816_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4920752.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7970..9400
                     /gene="gatB"
                     /locus_tag="ACG816_001879"
     CDS             7970..9400
                     /gene="gatB"
                     /locus_tag="ACG816_001879"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4920753.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9410..10465
                     /locus_tag="ACG816_001880"
     CDS             9410..10465
                     /locus_tag="ACG816_001880"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4920754.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10576..11949
                     /gene="rlmD"
                     /locus_tag="ACG816_001881"
     CDS             10576..11949
                     /gene="rlmD"
                     /locus_tag="ACG816_001881"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4920755.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11952..12080
                     /locus_tag="ACG816_001882"
     CDS             11952..12080
                     /locus_tag="ACG816_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4920756.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12520..12708
                     /locus_tag="ACG816_001883"
     CDS             12520..12708
                     /locus_tag="ACG816_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4920757.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12786..13799
                     /locus_tag="ACG816_001884"
     CDS             12786..13799
                     /locus_tag="ACG816_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4920758.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13866..14900)
                     /locus_tag="ACG816_001885"
     CDS             complement(13866..14900)
                     /locus_tag="ACG816_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4920759.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15015..17241
                     /locus_tag="ACG816_001886"
                     /pseudo
     CDS             15015..17241
                     /locus_tag="ACG816_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
     gene            17259..19142
                     /locus_tag="ACG816_001887"
     CDS             17259..19142
                     /locus_tag="ACG816_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4920760.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19123..20097
                     /locus_tag="ACG816_001888"
     CDS             19123..20097
                     /locus_tag="ACG816_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4920761.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6838 a         3720 c         4343 g         5387 t
ORIGIN      
        1 ttttcccagc ctcttctttt tttatagaat catatagaat caagtagtgt agatgaatag
       61 ttgttgtaaa atagtttttc catgttcgtt aaaatgattg cttcaaattt gtgatagaag
      121 atttagcctc gatttcctgc ttgattcttg gcaagtttgc ttcatcagtc aagataacca
      181 aaatatctcc tacattcatg atcgtatcac cgtgagtcaa gatttcctga tttcctctgc
      241 gtatagagat tagcagcata tttttaggcc aattgaagtc tcgtaccatt aatccgtcta
      301 aagaactctc tgccgttact ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg
      361 tctgagtatt tccaagtaga cgttccaata atgattcgta aattggatta ccgccgagaa
      421 ggtcattgac gatataagca acaagagcag ccaatcctaa aggcatcaga tgattaagag
      481 agccaaccat ttctgtcact aaaataattg ctgtcagcgg tgcttttcca atcgctgtga
      541 aataaccagc catggcaata acgacaaaat ccttgatgta gattggatcc atcccgaaat
      601 caaccataat gtttccataa aagctaccga ttaaagcccc caatgtcaat atcggaagga
      661 aaatcccccc tggaagaccc ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa
      721 acaaaccgat aatcataatg attggcaagg aagcatgtcc aaaagctaaa acgatttgat
      781 tgcctccgcc tagagtacta ggccataaat aaccaattgg caaaatcagc aagaaaggaa
      841 cgaacccgtg aaaataagct ggtaaaggca atcggttgta aaattttggc aaagccagca
      901 agacattctg ataaataaaa cctaagatgc ctagaagtac tcctaagatc actaaggaac
      961 cataatattt caacggcaaa gaaggaaggt cacctagata taaaacaggt ctcaagccga
     1021 aaaagtaaag agagacgaag ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa
     1081 gtggagaaaa ggagtgatgg atttcttcta aaacaaagag taatcctgcg ataggagcat
     1141 tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat taaaatcttt tcttctgaag
     1201 atggactccg aaacaattgt ccagctcctt gtccaatgga tgcgccaagc tgtatagaag
     1261 gtccttctct acctagaaaa aggcccgcac caactgaaag tacaccgcca atgaactttt
     1321 tccataaaac agagaaccag ttcattgata attccccgcg aagctgtccc tctacttgtg
     1381 gaatcccgct tcccttgata tccggttcat ttttgaccag ataacccacg aaaaatgctg
     1441 caaggatgca gcaaatagct aggccaatta agtaaatcgg ctgttcccga ctcatttcat
     1501 agatacttcg aactaaatcg cttagctttt cgatagataa tcgaaacagg ctaacgacga
     1561 ttcctgtcag cagtccaaca aatattcctt ttagaataaa atatatctga gtcttatcta
     1621 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
     1681 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
     1741 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
     1801 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
     1861 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
     1921 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
     1981 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
     2041 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
     2101 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
     2161 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
     2221 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
     2281 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
     2341 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
     2401 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
     2461 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
     2521 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
     2581 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     2641 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     2701 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     2761 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     2821 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     2881 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     2941 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     3001 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     3061 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     3121 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     3181 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     3241 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     3301 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     3361 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     3421 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     3481 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     3541 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     3601 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     3661 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     3721 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     3781 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     3841 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     3901 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     3961 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     4021 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     4081 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     4141 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     4201 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     4261 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     4321 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     4381 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     4441 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     4501 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     4561 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     4621 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     4681 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     4741 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     4801 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     4861 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     4921 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     4981 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     5041 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     5101 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     5161 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     5221 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     5281 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     5341 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     5401 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     5461 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     5521 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     5581 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     5641 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     5701 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     5761 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     5821 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     5881 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     5941 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     6001 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     6061 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     6121 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     6181 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     6241 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     6301 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     6361 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     6421 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     6481 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     6541 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     6601 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     6661 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     6721 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     6781 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     6841 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     6901 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     6961 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     7021 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     7081 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     7141 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     7201 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     7261 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     7321 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     7381 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     7441 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     7501 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     7561 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     7621 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     7681 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     7741 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     7801 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     7861 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     7921 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     7981 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     8041 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     8101 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     8161 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     8221 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     8281 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     8341 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     8401 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     8461 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     8521 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     8581 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     8641 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     8701 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     8761 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     8821 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     8881 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     8941 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     9001 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     9061 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     9121 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     9181 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     9241 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     9301 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     9361 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     9421 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     9481 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     9541 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     9601 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     9661 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     9721 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     9781 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     9841 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     9901 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     9961 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
    10021 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
    10081 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
    10141 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
    10201 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
    10261 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
    10321 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
    10381 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
    10441 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
    10501 cggttttagt tgtgtaatta gctgatttat agtagagttc agtaggattg aaaaataggc
    10561 ttaaaggagc aaaatatgac agaacagcaa atagtaaaga aaaacgaacg atatacagtc
    10621 gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa aaatcgatgg ttatccatta
    10681 tttatcgaaa atgcactccc tggagaagaa gtagaaatac tagtagtaaa agcaggcaat
    10741 aagtttggat atggaaaagt cgaaacattt attacacaat cacctgatcg tcagcctgta
    10801 gaaaacaatg cattacttcg cataggtatc gcacctttag cccatttgaa gtatgaacaa
    10861 cagcttgtct tcaaacaaaa acaagtagag catgtaatgg cgaagattgc taagatgcca
    10921 gaagttcctg tacttccaac ggtcggaatg gcagaaccat tcggctaccg taataaagca
    10981 cagatccctg ttcgccgtat cgatggacag ttagccaccg gtttttacaa aaagaatagc
    11041 catgatctag tccctatcga agatttttat atccaagatc ctgtcatcga tcaagcaatc
    11101 aaagtagtca gagatatctt gcaacggttc caagtacgag gatataatga agaaaaaaat
    11161 gaaggacagc ttcgtcacat cattatccgt cgtggtcatt attcccatga aatgatggtc
    11221 gttttagtta ctcgtaaaga aaaattcttt aaatccaagg aaattgctga agctattcat
    11281 gaagaattgc cagaagtggt ttcggtcatc caaaatatca atgaagaaaa aacaaatgtg
    11341 attttaggag acaaagaaaa agttttatat ggcagagact atatcgaaga tcagctatta
    11401 ggaaaaacct atcgaatctc agcaaagtca ttttatcaag taaatactca acaagcagag
    11461 tatctgtacc aaacagccat cgagtttgcc gagttagata aaaaagacat tgtgatcgac
    11521 gcttattcag gaataggtac gattggcttg tctttagcag acagagtgga taaggtgtat
    11581 gggatggaag ttattccaga agcaattgaa gatgcacagt tcaatgcttt aaataataaa
    11641 atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag ttatgaaaaa atggcaagaa
    11701 aaagggatca aaccatctgt gatcgtagtt gaccctccac gaaaaggatt agatcatcat
    11761 tttattgaat cagcaatcga aatgtcacca gaacgaatcg tctatatctc atgtaatccg
    11821 gcaacttttg caagagatgc taagttattt gcagaagcag gttatgccgt gaagaaagtt
    11881 cagcctgtag atcttttccc gcaaacccat catatcgaat tagtcgcttc attcgttaaa
    11941 ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt gatcaaaaag aaacaaggtg
    12001 gaggccgatc caaacaaatg gaaacaccta agaatgatcg taaaaacatg agaaaaggcc
    12061 ctaaaatctt taataaatag acgtgctaaa gaaagactaa gaagtgttaa cttttgttat
    12121 tgtttcttag tctttttagt tagtctacat tattttggcg atttgccaat aaggactgtt
    12181 gagttgtgca aaatcaaatc tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt
    12241 atttcaattt gattaactga ttgatcaaaa agatttttat tttcatattc tcaaatgatt
    12301 caacactaaa tatacatgtt tgacgcggaa attattattc gattttggcg gcatcagggg
    12361 ggatgacgac gtagcagacc cattatttta ctcaagaaaa tgtccgtgcc tattatttag
    12421 aatacgatag tgaacgtgct gggagttttg ttcttttaga gaaagtctct gaagataaat
    12481 tagttgtctt agattttgac tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg
    12541 attgaccgga tctacgaagc gacacgatat ctgcctttgg atcgtttgtg cttaagtcct
    12601 caatgtggat ttgcttcaac agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa
    12661 tttcttttgt caaaagcatt gcagaagaag tttggggaaa atcattaaat catctaggag
    12721 agcttgatag gaaataacac tatggtataa tcagaatgct taatactaac tactgaggga
    12781 gccttatgaa aaagaaaatt cttaaattta tcggatggtt cttcggaatc attcttggga
    12841 tcgcgtttgc aatctttctt gccttccagc tttcccccag acctggagca ttcttgatca
    12901 accatatgtt ttcaaatact gttcaaatca ctgataaaaa aacgttcgat caagcagaaa
    12961 aaaacgtggc gaaaaaaaca gatttgacgt atgagtccaa gtataaagag aatacttatg
    13021 acatctacta tccaaaaaac agcaaggggc ctgttcctgt tctattttgg gtacatggtg
    13081 gaggattcgt cggcggagat aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag
    13141 acgccaacat cgcagtggtt gcgatgaatt atgaattagc gcctgattca gaatacccta
    13201 atcaagttct tcaagtgaat gaactgatca aagatttatt agctgaagaa aaagacgaca
    13261 aattgctaga catggaacaa ttattttttg gtggagatag tgcaggttca caaatcgcat
    13321 tgcaatatgc agctatacaa acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc
    13381 tacttccaaa agatagtctg aaaggaacca tttcttattg cggacctgtt gatttgaagc
    13441 taacagcaca gcagcattcg gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt
    13501 ggtcgttgat tggaacaaaa gattggaaaa cgagtgagca attgaaagaa gctagtgtgg
    13561 ctgaccatgt ttctaaggaa tttccgccaa cgtatataac tgacggaaat gcatactcat
    13621 tccaggagca aggtcaagca ttagaaaatc ggttgacaga attaaaggta cccgtgcaat
    13681 ccttgtttta tacagataca aaaaaagaga ttacccatga ataccaattt gattacacgc
    13741 taaaagaatc gcaaaattgt taccagcaga cattggattt tgtgaataag tataagtaag
    13801 aggttgtgaa aaagccgttt cgttttgagt atgaagaaac gaagcggctt ttcacaacct
    13861 ctctttcaat acctttttgt tcttgggata aaactttccc taacaatcaa tcgtgcagga
    13921 taagtcaaaa tgattggttc gtcccgtata ccgtcaattc gctccaatgc cattcgaaca
    13981 gctgcttgcc caattgattt cgcattgatt tgaacactgg tcaagccagg ggagaggttt
    14041 gcgattgtct cgatattatc aaagcttgct acaacaatct cttctccaat ttggatattt
    14101 ttttgtttca atgcccgata gacaccaatt gccattggat cgctcccgac aagaatggca
    14161 gtaggaagag gggattcatt ttctaataga ttagtcgcaa gtacctcacc ttgtttttct
    14221 gtccagccac ctagatgata agacatatat ttttctaagt gatgatcgat cataaaatgc
    14281 tgataggcac gcatcctctt ttcatttata gaaatctgct tcccaccgaa ttcatccata
    14341 tcgactttat atcccccgat ataggctatt cttttatgac cattttctaa gaacaattcc
    14401 agtacggttt tagtcatccg ttcaagatct gcatacacca tatcgacctc ttgctggatg
    14461 tcagggttat ccacgacgac tatttgcatg ttttgtttta acaaggcggt aatcgattct
    14521 ttcgtaacag tcccaacaac gatcaccgca cctaactgat ctagatcttc ccattgttta
    14581 ggattatcag atagattata gattcgattc atccctaaat gcagacgtcg agcttcttct
    14641 tccatcccta ctcgcaaatg tttgtaatag atatcctgtt cttcgctcgc tttgccgatc
    14701 gtagagatca caccaatcga aggcattctc tttttgatat atttacgagt actgatgtca
    14761 taatccaacg cagcagctgc ctctactatc cgttgccgag tagtctgagc cacatggatc
    14821 gtctgatcgt tattcaacac acgggaaact gtagcaggag agacatttgc ctttcgtgcg
    14881 acatctctaa ttccaaccat ttttacacac tcctcaaaaa aataaaaaat ttttattgac
    14941 aaaattgttt agtaaacagt atacaggaat tataaaaaaa agtaaacggt ttccgttttg
    15001 ttgggagtga aatgatggat attacgcata gaaaaacaga taaaaatttc atcttatttg
    15061 atgaaaagag ccgagtcttc catttacgaa atacatttct ttcttatctg ataaagatag
    15121 aagaaagcaa tgtattggcg catatttatt ttggcaaacc agtcaagcag tacaaagaca
    15181 ataaaaatta tccccgaaga gatcgtggat tttcaggaaa tgttccctta aatcctgacc
    15241 ggtctctgtc gaaagataca ctaccgcaag aatactcttc tcatggcagc atggactttc
    15301 gaacgcccgc atcgatcatt cagcgcaaga atggatcaga cttactggat ctgagatatg
    15361 acagccatta tattacagat gggaagccag agatcgaagg cttgcctcaa acttatgtga
    15421 tggacaagtc agaggcacaa acgttggtga tttcacttaa ggatagagaa acagcgattt
    15481 atttcgattt gttctatacc atatttactg atagagcagt gatcacacga tctgtaaaga
    15541 taagaaatga aaccggagaa acaatcaaac tagaaaaagc ggctagtttc cagctagact
    15601 ttgcccatac taggcgattt gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa
    15661 gacagatttc acggcaatct gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa
    15721 cgagcagtca ccatatgaat aactttattg ctttagttca tcatcatacg acagaaaata
    15781 ctggagaagc gatcggactt caatttgttt attccggcaa ccattctttc gaacttgaaa
    15841 aagatcagat caatcaattg agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg
    15901 aattgaatgc aggccagtct tttcaaacac ctgaaatgat cctttcttac tcaagtcagg
    15961 gattgaacaa aatgagtcag atccaccatg agttgctacg tgaaagaatc gcaagaggtc
    16021 gtcatcaatt tgctgaacgg ccgattctgg tcaataactg ggaagccact tactttgact
    16081 tcaatagtga aaaaatcaag gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt
    16141 tcgtattaga tgatggctgg tttggaaaaa gagatgcgga caattcttca ttaggagact
    16201 ggttcgagta tgaaggcaag ttgacaaatg gattacgtga aatagcagat tatgctcata
    16261 gcaaaggatt gaaatttggt ttatggtttg aacctgaaat gatctcggtt gattctgaac
    16321 tgtatcgaac acatcctgat tttcttatgc aggagccagg tagaatgcct tctgcttcca
    16381 gatcacaaca tgtactggat tttacaaggt tagatgttcg gcagacgatt gagaaacaaa
    16441 tgagaaagat tttagatacg attcctcttg actacatcaa atgggatatg aaccgttcgc
    16501 tttcagatgt ctattcgatt acccttgacc cacagagaca aggagaagta gcgcatcgtt
    16561 atatgctcgg gctttatgaa ctacttgaac atttgacaac tgattatccg gaaatcttat
    16621 gggaaggctg ttctggtgga ggtggacggt ttgatgccgg gtttatttat tatatgccgc
    16681 agtcatggac aagtgataac acggatgcag tggagcgtat gaaaatccaa tatggaacaa
    16741 gccttgctta tccgatttcg tccatcacag ctcatgtatc agctgtacca aatcatcaga
    16801 ccgacagatc tacttcgttg aaaacaagag gagaaacagc tatgagcgct gtttttggtt
    16861 atgaactgga tttgacaaag ctgtctcctg aagaaaaaaa gcaagtaaaa gaccaaatta
    16921 tatcctatca aacgattcgt ccggtgatcc agtatggcca ttattatcgt ttggcgagtc
    16981 cttttgaaga aaatattgcc gcatggatgt ttgtgtctcc taaacaagat gaagctattg
    17041 tgttttttag ggaggatact agcttcagct caaccagcgt tccatgaagt atatctgatg
    17101 gggttagatg atgaggcact ttatcaggaa cagacctcga agcggatatt ttcgggggcc
    17161 gaattgatga cagttggact ttacttcccc gattttcaag gtgatttcca aacagaactg
    17221 cttcatttca aaaagttatg agagagaagg aaaaaagtat gaaagcaaat ataaaatggt
    17281 tagatgatcc acaagttttt agagtgaacc aatcaaatgc acatagcgat catgtttgtt
    17341 atgcaacaag ggaagaagta gaaaccacat cctcccttaa acagtcttta aatggatcat
    17401 ggcgtttttg ttattcgcca aatccttcgg tacgaccaaa aacattttat aaaaaagatt
    17461 ttgatgcctg ttcttttgat gaaatcactg ttcctagtca tattgaatta gcaaattatg
    17521 acaagatcca ttacatcaat acgatgtatc cgtgggaggg acatgtattt cgccgtcccc
    17581 ctttcacgac aagccctgag aaagaggcgg aagggatctt ttcagctgct aaagacaatc
    17641 cggttggttc gtatttgacg acttttcgat tagaagaagg tctaaaggga aaacaaatct
    17701 cgattcattt tgctggtgta gaacaagcaa tgtttatttg gttgaatggt cattttatcg
    17761 ggtatgccga agattcgttc actccgtctg aatttgattt aacaccttat ttggaagaag
    17821 aaaatattct agctgtggag gtttacaaaa gaagtacagc tgcattcttg gaagatcagg
    17881 actttttccg attttttggg atcttcagag atgtcttctt agttgctcga ggacaaaacc
    17941 atttagaaga tctttggatc aaacctcgct tagatgacaa atgtcaaaat ggcaaacttg
    18001 aagtagattt gtcttttgag caagtagcca aaaacagtca gctgaatgta gcgatactag
    18061 atcctgatgg gaaggaaatc tatcaacagc aatatccgtt aaaagagcaa atgacgattc
    18121 gcatcgatgg atttgaaaat gtggcactgt gggggcattt tcagcccaat ttatataagc
    18181 ttgaattgga gacgatagac gaagaaaaaa gagtaacgga gtatattgac tattccttcg
    18241 gttttcgtaa aatcgaaatc aaagataaaa tcatctactt caatggaaaa cggatgatta
    18301 tcaacggggt aaatcgacat gagtggcatc cgaaaaaagg acgcgccatc actatagaag
    18361 atatggaaaa agatttggct atcataaaaa atgcaaagat caacggcgta cggacatctc
    18421 attaccccaa ccagatcact tggtattatt tatgcgacca agcaggaatc catgtgatgg
    18481 cggaaacgaa cctcgaatcc catggctcat ggcaaaaaat gggcgcattt gagccttctt
    18541 acaacgtacc aggtgcagta cctcagtggc aggcggctgt tcttgatcga gcaaaaacga
    18601 atttcgaaac attaaaaaac catccaagta ttcttttttg gtctcttgga aatgaatctt
    18661 atgcggaaga aaacattcga caaatgaacg attacttcaa aaaagtagac cccgatcgac
    18721 ttgtccatta tgaagggaat tttccaaatc cagcattcga agattcgatt tcagatgtaa
    18781 aaagctgcat gtatgcgcca ccagaagaga tcattgacta ccttgaaaat aaagcggaaa
    18841 aaccgttcat cttatgtgaa tatatgcatg acatggggaa ttcgttaggt ggaatggatt
    18901 cttacatgac gttgcttgat cgttatgaaa tgtaccaagg aggctatata tgggatttta
    18961 aagatcaggc gatccaagtg actgatccaa tcacaggcag agaagtgatg cgctatggcg
    19021 gtgactttga cgataaacca agtgactatg aattctcagg gaatgggatc gtttttgcag
    19081 atggacaaga aaaacccgcc atgcaggagg taagatatta ttatgaaaaa tacagtaaat
    19141 aaaagtcata tggatacgga aaaagttgca atcgtcttcg gcgactgtac attaggtgtc
    19201 aaatcgggga atacgcatta tattttttct tatacaagag gcggactgga atcgctcaat
    19261 aaaaacggaa aagaatggct ataccgggaa acacttccag ttttttggcg agcaacgacc
    19321 gataatgaca gaggtagcgg atttggattt aaatccggta tgtggttagg agccggattg
    19381 tatccaaaag tcaaagaaat agaagtactg gtggacaata aaaagatcga tttgccgatt
    19441 gctccagtaa ataatcaata ttcaagtaat gaatacgctg aaacggttga aatcattttt
    19501 acactggaaa tcaacacagt accaaaaacc caagtgacaa tttcctacca aatcgctgga
    19561 gagggtaaaa tgcttgtcaa tatgcattat tatggacaaa aaggattacc ggaattaccc
    19621 gcattgggtt taaggttcat tatgccaacg aaagcaaaat tttttgagta tgaaggccta
    19681 tcaggggaaa catatccgga tcggatggct ggtggtgtcg aaggtattta taaagtggca
    19741 ggactccccg tttccccata ccttgttccc caagaatgcg gcatgcatat ggaaacgaaa
    19801 tgggtagaac taacaagaaa tcaaacgttg gataatacgg ataaagatca agaagatttt
    19861 tcgttgcgat ttgaacagca ggatacgcca tttgcttttt ccgctttacc attcaaagca
    19921 gaagaactgg aacaggcaac acatatagaa gaactaccgc cagaaagacg aacggttctt
    19981 acgatttttg caaaagtaag aggagtcggg ggtatcaata gctggggagc ggatgttgag
    20041 tcaccttacc gaatcagcag cgaagaggac catgaaatcg gctttattat tcagtagcaa
    20101 tctttagatt attatttgaa aaacggagtt tacaagacaa agctgcttta ttctatatgt
    20161 catgaacaag agagtaaaaa agttctcaga tacttttgtt catgactatt ttcttcaata
    20221 aaaccaattg ttatttttct gaaaaacaaa gttaactatc agaaaattaa aaaaagtttc
    20281 ataaaaat
//
LOCUS       DAZUKT010000053        20183 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000053 DAZUKT010000000
VERSION     DAZUKT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.054"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="ACG816_001889"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="ACG816_001889"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4920762.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="ACG816_001890"
     CDS             1013..2260
                     /locus_tag="ACG816_001890"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4920763.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="ACG816_001891"
     CDS             2336..2680
                     /locus_tag="ACG816_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920764.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="ACG816_001892"
     CDS             complement(2797..3123)
                     /locus_tag="ACG816_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920765.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="ACG816_001893"
     CDS             complement(3496..4698)
                     /locus_tag="ACG816_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4920766.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="ACG816_001894"
     CDS             4815..5330
                     /locus_tag="ACG816_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4920767.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="ACG816_001895"
     CDS             complement(5529..5975)
                     /locus_tag="ACG816_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4920768.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="ACG816_001896"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="ACG816_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4920769.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="ACG816_001897"
     CDS             complement(7854..8483)
                     /locus_tag="ACG816_001897"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4920770.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="ACG816_001898"
     CDS             complement(8467..8931)
                     /locus_tag="ACG816_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4920771.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="ACG816_001899"
     CDS             complement(9418..10686)
                     /locus_tag="ACG816_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4920772.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="ACG816_001900"
     CDS             complement(10917..11654)
                     /locus_tag="ACG816_001900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4920773.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="ACG816_001901"
     CDS             complement(11721..12290)
                     /locus_tag="ACG816_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4920774.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="ACG816_001902"
     CDS             12418..13290
                     /locus_tag="ACG816_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4920775.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="ACG816_001903"
     CDS             13632..14504
                     /locus_tag="ACG816_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4920776.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /gene="nagE"
                     /locus_tag="ACG816_001904"
     CDS             14501..16459
                     /gene="nagE"
                     /locus_tag="ACG816_001904"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4920777.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="ACG816_001905"
     CDS             complement(16710..17561)
                     /locus_tag="ACG816_001905"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4920778.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="ACG816_001906"
     CDS             17684..18151
                     /locus_tag="ACG816_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4920779.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="ACG816_001907"
     CDS             complement(18414..18896)
                     /locus_tag="ACG816_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4920780.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="ACG816_001908"
     CDS             complement(18996..19859)
                     /locus_tag="ACG816_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4920781.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6068 a         4157 c         3662 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc ttt
//
LOCUS       DAZUKT010000054        19507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000054 DAZUKT010000000
VERSION     DAZUKT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.055"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG816_001909"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG816_001909"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4920782.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG816_001910"
     CDS             complement(1173..1868)
                     /locus_tag="ACG816_001910"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4920783.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG816_001911"
     CDS             complement(1881..2597)
                     /locus_tag="ACG816_001911"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4920784.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG816_001912"
     CDS             2927..3532
                     /locus_tag="ACG816_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4920785.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG816_001913"
     CDS             3857..4180
                     /locus_tag="ACG816_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4920786.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG816_001914"
     CDS             4362..5732
                     /locus_tag="ACG816_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4920787.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG816_001915"
     CDS             complement(5862..7163)
                     /locus_tag="ACG816_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4920788.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACG816_001916"
     CDS             complement(7393..9384)
                     /locus_tag="ACG816_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4920789.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACG816_001917"
     CDS             complement(9528..9794)
                     /locus_tag="ACG816_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4920790.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG816_001918"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG816_001918"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4920791.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG816_001919"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG816_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4920792.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACG816_001920"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACG816_001920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4920793.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACG816_001921"
     CDS             complement(13954..14475)
                     /locus_tag="ACG816_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4920794.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACG816_001922"
     CDS             complement(14478..15320)
                     /locus_tag="ACG816_001922"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4920795.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACG816_001923"
     CDS             complement(15313..16038)
                     /locus_tag="ACG816_001923"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4920796.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACG816_001924"
     CDS             16214..16510
                     /locus_tag="ACG816_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920797.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACG816_001925"
     CDS             17078..18670
                     /locus_tag="ACG816_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4920798.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACG816_001926"
     CDS             18683..19423
                     /locus_tag="ACG816_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4920799.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAZUKT010000055        18655 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000055 DAZUKT010000000
VERSION     DAZUKT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.056"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..278
                     /locus_tag="ACG816_001927"
                     /pseudo
     CDS             150..278
                     /locus_tag="ACG816_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            392..631
                     /locus_tag="ACG816_001928"
     CDS             392..631
                     /locus_tag="ACG816_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920800.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            743..937
                     /locus_tag="ACG816_001929"
     CDS             743..937
                     /locus_tag="ACG816_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920801.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1023..1694
                     /locus_tag="ACG816_001930"
     CDS             1023..1694
                     /locus_tag="ACG816_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4920802.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1747..3723
                     /locus_tag="ACG816_001931"
     CDS             1747..3723
                     /locus_tag="ACG816_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4920803.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3738..4490
                     /locus_tag="ACG816_001932"
     CDS             3738..4490
                     /locus_tag="ACG816_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4920804.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4506..5261
                     /locus_tag="ACG816_001933"
     CDS             4506..5261
                     /locus_tag="ACG816_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4920805.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5274..5534
                     /locus_tag="ACG816_001934"
     CDS             5274..5534
                     /locus_tag="ACG816_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920806.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5531..7621
                     /locus_tag="ACG816_001935"
     CDS             5531..7621
                     /locus_tag="ACG816_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS4920807.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7648..8112
                     /locus_tag="ACG816_001936"
     CDS             7648..8112
                     /locus_tag="ACG816_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920808.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8109..8351
                     /locus_tag="ACG816_001937"
     CDS             8109..8351
                     /locus_tag="ACG816_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS4920809.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8369..8671
                     /locus_tag="ACG816_001938"
     CDS             8369..8671
                     /locus_tag="ACG816_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920810.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8741..10804
                     /locus_tag="ACG816_001939"
     CDS             8741..10804
                     /locus_tag="ACG816_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920811.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10804..13416
                     /locus_tag="ACG816_001940"
     CDS             10804..13416
                     /locus_tag="ACG816_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4920812.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13413..15944
                     /locus_tag="ACG816_001941"
     CDS             13413..15944
                     /locus_tag="ACG816_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4920813.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15994..16326
                     /locus_tag="ACG816_001942"
     CDS             15994..16326
                     /locus_tag="ACG816_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920814.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16327..16920
                     /locus_tag="ACG816_001943"
     CDS             16327..16920
                     /locus_tag="ACG816_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920815.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17083..>18655
                     /locus_tag="ACG816_001944"
     CDS             17083..>18655
                     /locus_tag="ACG816_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS4920816.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         6891 a         2925 c         3836 g         5003 t
ORIGIN      
        1 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
       61 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      121 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aatgacgctt acgaatgata
      181 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      241 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      301 agataaaggc ttagaagaag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      361 gagtaaggaa ttcatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      421 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      481 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      541 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      601 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      661 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      721 cagaaaaaaa gtgtcgaaat aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      781 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      841 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaac
      901 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      961 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
     1021 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     1081 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
     1141 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
     1201 tcaataatga tatgtctttg gggcaaatgt tgtccataca acaagaagca atcgaaatgg
     1261 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
     1321 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1381 atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1441 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1501 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgagtac tttaaattaa
     1561 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1621 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1681 gagaactaca ataacaaggt tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag
     1741 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttattgg aatgttagga
     1801 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     1861 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1921 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1981 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     2041 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     2101 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     2161 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     2221 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2281 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2341 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2401 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2461 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2521 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2581 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2641 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2701 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2761 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2821 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagt
     2881 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
     2941 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     3001 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     3061 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     3121 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     3181 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3241 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3301 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3361 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3421 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
     3481 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
     3541 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
     3601 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
     3661 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3721 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
     3781 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
     3841 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     3901 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     3961 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     4021 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     4081 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     4141 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     4201 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     4261 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     4321 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     4381 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     4441 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     4501 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     4561 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     4621 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     4681 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     4741 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     4801 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     4861 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     4921 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     4981 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     5041 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     5101 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     5161 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     5221 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     5281 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     5341 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     5401 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     5461 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     5521 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     5581 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     5641 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     5701 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     5761 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     5821 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     5881 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     5941 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     6001 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     6061 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     6121 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     6181 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     6241 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     6301 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     6361 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     6421 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     6481 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     6541 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     6601 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     6661 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     6721 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     6781 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     6841 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     6901 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     6961 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     7021 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     7081 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     7141 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     7201 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     7261 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     7321 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     7381 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     7441 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     7501 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     7561 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     7621 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     7681 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     7741 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     7801 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     7861 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     7921 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     7981 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     8041 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     8101 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     8161 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     8221 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     8281 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     8341 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     8401 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     8461 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     8521 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     8581 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     8641 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     8701 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     8761 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     8821 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     8881 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     8941 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     9001 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     9061 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     9121 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     9181 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     9241 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     9301 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     9361 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     9421 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     9481 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     9541 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     9601 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     9661 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     9721 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     9781 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     9841 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     9901 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     9961 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
    10021 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
    10081 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
    10141 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    10201 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    10261 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
    10321 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
    10381 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
    10441 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    10501 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10561 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagcccaaga
    10621 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10681 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    10741 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10801 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    10861 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    10921 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    10981 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    11041 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    11101 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    11161 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    11221 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11281 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11341 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11401 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11461 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11521 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11581 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11641 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11701 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11761 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11821 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11881 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11941 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    12001 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    12061 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    12121 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    12181 ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12241 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12301 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12361 ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa cttattagta
    12421 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12481 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12541 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12601 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12661 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12721 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12781 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12841 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12901 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    12961 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    13021 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    13081 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    13141 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    13201 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    13261 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13321 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13381 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13441 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13501 aagtcttaca agattatggt aattatctgc agtatcaaga tgtatttagt tggatttgga
    13561 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13621 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13681 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13741 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13801 ttatggccat gatcttgatt tttggtggga gttctttaat cgatgaaggg ttaaatatca
    13861 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggttcacctg
    13921 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac attactagca actgacccga
    13981 ataaagtaga atccctgcca acagacaaaa gaaatgcttt aacagcgaaa aattttaaaa
    14041 cagcgaatat caatgggatt attactccta aacaagctaa agaaatggcg gataaagata
    14101 agagtggttt atcagattta caaaaggatc ggttaagagc gttacaatac cgattggaac
    14161 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    14221 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatctta gctggtgaat
    14281 cttctttggc ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14341 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggccaac
    14401 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14461 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14521 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    14581 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagtaatt
    14641 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14701 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14761 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14821 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14881 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14941 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    15001 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    15061 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtt aatgcagcag
    15121 taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    15181 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15241 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15301 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15361 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15421 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    15481 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15541 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15601 atagtcgttt gaatcaattc aaggagaatg atcaaagctt tgttcaccaa tcaactgcag
    15661 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15721 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15781 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15841 ttactgcacc aacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgcattga
    15901 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    15961 gccttttatt tatatgaaaa ggagaaatca attatggata aatatataaa catgaaaaac
    16021 cctccaagat ttagtggaga atggaagttt ttcgcttttt tctttttaga tctattaacg
    16081 cttggtggct gctttttttg ggcgaaaatg gtcaataatc ttgtccactt accactcttt
    16141 tttgcactct tgaattatgg cttatggatt gcgatagcgt tagtgattgt ttggcgaccc
    16201 tataataacc caggtcaacg acaaattttt gcgattatcg agtctatttt ttttgcggac
    16261 aagaaccatt atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
    16321 gaataaatgc gagtcaaagg acaaaattca gttgaaagga aatccatttt gcagtttgga
    16381 aaaccagaag agcgctctat cttggactta tttccgtata aatcctgtac agaagaggga
    16441 gtcttaatta ctagagaaga tcattttcaa cgttattatc gtgtagtttc tacagacgtg
    16501 gaaggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
    16561 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
    16621 atcgtgcaaa agcggcaatt attagataaa aatcgtatgg aacagtcctt aggaagaaat
    16681 ctacaacgat taaaaaaata tgaaaagaaa ttagtcgaag aaatccagga attgaaacga
    16741 gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa gacaatcaag
    16801 gatttacaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    16861 ctctacaaaa aagaattgat caagattctg tatcgaatga acaatatgaa tgatgagtag
    16921 gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaagga
    16981 agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa aagggcaatc
    17041 ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa
    17101 acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca actaggaaat
    17161 cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa acaagtgatg
    17221 ccacaaggtg ggttaaattt tgatgaagaa tatgtgacgt atggtaatgg ttattcacga
    17281 tgtgtgacag tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg
    17341 aacaatcctc atacagtggc cacactcgat gttagcacag atgaaaagca aaaagtgatt
    17401 gatcaaatca atcgtgcctt agatgaatta aatgatcgat cagataatga acgaaaagcg
    17461 actgaccgaa ataaaagcgc agaagaatac ctggaactag cggaatatgc tcgtaaattg
    17521 acttctaatg gggaaatttc taagcaagtc aaagtgagag tacatgttta cggaaacacg
    17581 attcaagaag tagatgaacg agcgcaggaa ctgatcgaaa atctaaaagg gatggattat
    17641 cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc
    17701 gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc gttaaacatt
    17761 ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggagggac acaatatggg
    17821 gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag tgtacggttg
    17881 tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg
    17941 atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac
    18001 tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg atctgacgga
    18061 atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc cagtttatat
    18121 gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacacactta
    18181 gacaaagtgg tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca
    18241 gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt
    18301 acttcctcaa attcgcgttc aaatacggag acgcaagtca caggtaaaga tccagaagct
    18361 tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc
    18421 caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg
    18481 tatggcatga tttttgatgg gcatagtacg attcgacacc ttgaccaaca acagttggtc
    18541 ttttttgaca ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac
    18601 atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgac
//
LOCUS       DAZUKT010000056        17975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000056 DAZUKT010000000
VERSION     DAZUKT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.057"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..660)
                     /locus_tag="ACG816_001945"
     CDS             complement(<1..660)
                     /locus_tag="ACG816_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4920817.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GK"
     gene            complement(653..2005)
                     /locus_tag="ACG816_001946"
     CDS             complement(653..2005)
                     /locus_tag="ACG816_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4920818.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2228..3628)
                     /locus_tag="ACG816_001947"
     CDS             complement(2228..3628)
                     /locus_tag="ACG816_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4920819.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3789..4526
                     /locus_tag="ACG816_001948"
     CDS             3789..4526
                     /locus_tag="ACG816_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4920820.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4651..5130
                     /locus_tag="ACG816_001949"
     CDS             4651..5130
                     /locus_tag="ACG816_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4920821.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5127..5894
                     /locus_tag="ACG816_001950"
     CDS             5127..5894
                     /locus_tag="ACG816_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4920822.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6124..6693
                     /gene="lepB"
                     /locus_tag="ACG816_001951"
     CDS             6124..6693
                     /gene="lepB"
                     /locus_tag="ACG816_001951"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4920823.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6797..7399)
                     /locus_tag="ACG816_001952"
     CDS             complement(6797..7399)
                     /locus_tag="ACG816_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4920824.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7505..7735
                     /locus_tag="ACG816_001953"
     CDS             7505..7735
                     /locus_tag="ACG816_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920825.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7726..7974
                     /locus_tag="ACG816_001954"
     CDS             7726..7974
                     /locus_tag="ACG816_001954"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4920826.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8080..8664
                     /locus_tag="ACG816_001955"
     CDS             8080..8664
                     /locus_tag="ACG816_001955"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4920827.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8667..9740
                     /gene="prfA"
                     /locus_tag="ACG816_001956"
     CDS             8667..9740
                     /gene="prfA"
                     /locus_tag="ACG816_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4920828.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9733..10572
                     /gene="prmC"
                     /locus_tag="ACG816_001957"
     CDS             9733..10572
                     /gene="prmC"
                     /locus_tag="ACG816_001957"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4920829.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10565..10744
                     /locus_tag="ACG816_001958"
     CDS             10565..10744
                     /locus_tag="ACG816_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920830.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10766..11791
                     /locus_tag="ACG816_001959"
     CDS             10766..11791
                     /locus_tag="ACG816_001959"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4920831.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            11982..13226
                     /gene="glyA"
                     /locus_tag="ACG816_001960"
     CDS             11982..13226
                     /gene="glyA"
                     /locus_tag="ACG816_001960"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4920832.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13379..14326
                     /locus_tag="ACG816_001961"
     CDS             13379..14326
                     /locus_tag="ACG816_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4920833.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14460..15089
                     /gene="upp"
                     /locus_tag="ACG816_001962"
     CDS             14460..15089
                     /gene="upp"
                     /locus_tag="ACG816_001962"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4920834.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15228..15470)
                     /locus_tag="ACG816_001963"
     CDS             complement(15228..15470)
                     /locus_tag="ACG816_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920835.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15591..15663
                     /locus_tag="ACG816_001964"
     tRNA            15591..15663
                     /locus_tag="ACG816_001964"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15624..15626,aa:Thr,seq:cgt)
     gene            complement(15782..16420)
                     /locus_tag="ACG816_001965"
     CDS             complement(15782..16420)
                     /locus_tag="ACG816_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4920836.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16543..17175)
                     /locus_tag="ACG816_001966"
     CDS             complement(16543..17175)
                     /locus_tag="ACG816_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4920837.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17526..17825
                     /locus_tag="ACG816_001967"
     CDS             17526..17825
                     /locus_tag="ACG816_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4920838.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6166 a         3140 c         3550 g         5119 t
ORIGIN      
        1 tttaccgtaa cgttgttcta aagctaggcg gatcaaacga gctgccaggt tctcatttct
       61 tgcaaggata ggtccatgga aataagaacc gaagacattt cggtaaatga caccttcaga
      121 ttggtcttca ccattatttc cttgaccttg taccactttc cccaaaggtc gttcaccttc
      181 acctaagaaa gtccgaccat tgtggttttc aaatccataa taagtttcat tgaattcttc
      241 gttatggata acgatatcac cgataaaccg gttattttcc tgacttaacg tgtagtgatc
      301 cagtgcactg attccttgga tcttttctcc attagctccc atgtagtagt gacctagcaa
      361 ttggtagcca ccacaaatgg ctaacatgac gccatcattt tcgatatagt tgatcaaaga
      421 atctttcttt ttctggatgt cttttgaaat gattaattgc tcaaagtctt gaccaccgcc
      481 aaagaaaacc aagtcatatt tatcagcatc aaaaggttca tgtatactaa cgatctccgt
      541 atggcaagaa acccccattt tttctgctat atatttcaac atcaaaaggt ttccgttatc
      601 accatatgtg ttcatcaaat ttccataaag gtgggcgata ttgagttcat aattagccac
      661 ggttcattcc tcctttgata aatccttgag cagctagttc tttacgaagc tgcaatacag
      721 cagtgtaagt agctaaaata tagacatgct cagttggcaa ttctttgatt ttttcgatta
      781 cttctggtaa ttcttttgtt tctgtcaatt tattttctgg aatcccggca acttttagac
      841 gtaaagtcat atctgtatga cggtctcctc cagaaataac agctgggata tccatatcgg
      901 caaagttttc aaaattacca tcccaaatcc agctgacatc gattccgtct gcgtaattgg
      961 catttaataa agccactaaa gaaaatggat aaggagctag accaatcata tcgattactt
     1021 gattgatacc tacaggattt ttgacaagta ctaatgtaca ttttttccca ttgatattga
     1081 tgacttcttg tcggccgaat actttctcgt catatcctaa accttgtttg attttatcag
     1141 gatcgacatt gaagtactct gccactgcag tagctgctaa agcattgtag acattataca
     1201 taccgccaac ttcgattccg tattcatgac catcgatcac gaaatctgct gacacattgt
     1261 ccatccgtac catatcagtt aatcgataat ccaattcagg acgtttgaag tcacaatttg
     1321 gacagtaata tttccctaag tttgcataag tgatcatttt ataatgcaag atgtgatgac
     1381 aatttggaca taagacaccg tctgtattgt aatgagccat ttgttcacga tcttcttcat
     1441 ggtcaaagcc ataatatttt ctaggattga ctgtttcgat agaattgaag attggtgaat
     1501 caccgttact gataatcgtt gcatttggtg actttgcggc accgtctaca atcattttat
     1561 aggtcgtata gatttctcca taccgatcca tttgatcacg gaaaatattt gtaaataaga
     1621 acagttctgg tttcatatat tctgtgactt tgctcaaact tgcttcatct atttctagga
     1681 cagcaaactt tttctgtcct ttttttggtt ttgcctgcaa gaaagtcgat acaattccct
     1741 gtaccatatt cgcaccagta gggttggtta gaacttcatc gaattgctgg cgcaaaatat
     1801 tgactgttaa agcggtagtc aatgttttac cgtttgttcc ggtgacaacg acgacttggt
     1861 agtcttttgc caaactatct agtatatgtg gatcgacttt cagcgctagt ttacctggta
     1921 aacttgatcc gcctttaaaa aaagttttta atgcccattg cgacgttttt ccaactgcaa
     1981 tcgcaaaatg actgcgtatt cccattatgt aacctccaaa aacgatttgc tgacgaataa
     2041 attcaactaa tcataccata tgttgaaaaa aaggttaaat tgcttttctt attcttaaga
     2101 aaaggttacg gctgaaagtg catgaaatcg gacaaaatcg catgatttaa caaaaaaaca
     2161 gcttgaaaca aaatgattgt ttcaagctgc tgtgatttta ttattccctc ttacctatat
     2221 tcccatttta accgataata attttctcgc ttggatattc atagcctaga tctcgtgttt
     2281 cttttggcac gaataacatc aatgtatata gcattccgat ccgcccaata aacatcagtg
     2341 tagcaatcgt gattttgccc acaacagaca agtctccggt aatccctaaa gataaaccag
     2401 ttgttccaaa cgcagaagtt acttcgatga tgatcgagat cagcgtttgt tcttctgtag
     2461 cagataaaaa aacgatacaa aagaaacaca taccaagaga aagcataaag acaacgactg
     2521 attttcgcac atcatcttga tcgattctcc gtccaaaaat attgatgtta tcttcacttt
     2581 tgaggaagga atatagatac aaaccgatga ttgctacagt agttgttcga atcccgccgc
     2641 ctacagaact aggactgcat ccgataaaca tcagtaaaga aaaaatgatc agtgttgtga
     2701 tttgaaaatc acccaaatcg tgaatctgca gaccagcatt acgagtcgta atcgaataaa
     2761 acatcgaatt gatccatttg acactcatat tggaatcttg gaataaatgg tccttttcca
     2821 atagataaat caagacagtt cccgaaacaa acaagataac aaaagctaac acagctaatt
     2881 tggtaaacaa ggaaaaacga aacggaagct tcgcattgga acgtttgtat aatagccatt
     2941 cccggcactc catcaataca ggaaaaccaa ttcctccgac aaaaatcaaa aacatgatca
     3001 ggatcaagaa aaaatagtca tgtgcaaagg gcttgatgga atccccagtg acgtcaaacc
     3061 cagagttcgt gacagccgat atcgcctgat aaaaaccgta aaaaacggca tcacgccaac
     3121 gatcaaaata gcctctgtaa taaaaataaa tagaaaagaa tgtcccaaaa agtaattgaa
     3181 accagattaa aatagcaaaa gtgatccgaa tcagtcggac gataccagac aaacgcggtt
     3241 gattcatatc cgtcatgatc agctgtcgct gtttcagtgt gatccgtcgt ttagaaaaaa
     3301 tgacaaaagc agtagaaatc atcatgatcc ccaaaccacc aacttgaaat aatatttcca
     3361 gcagtataat accgttatca ttgaacacag aattgatatc aaaagtcgat agtccagtca
     3421 cactcactgt actgattgcc atgaacagca tgtccacaaa agaaacatgg ctatctggtt
     3481 cgcgaaaaat cggcatataa aatagaacga gtgataagac cgtcatcaat atataataaa
     3541 aaacgatgat ttggatcgat gagaaatgat tggcagcaaa cttctgtcct cgccgctgca
     3601 gccttcgaaa gaataaatca acatgcacag tactatcctc ctgacaatag tcgtttctac
     3661 cagtatacaa ggggaattag gaatactcaa agaaattatc aaacagataa caatacagct
     3721 ttcgattaaa atagaaatgg cttataataa ggaaaatcct agaaaaaaga aggaggagga
     3781 ataacaaatt gattaatgct caacaaatcg tagaaaaaat gaaaaatcaa aaaataaatt
     3841 atgatacagt tctgaaaaaa atgatcatgc aatgggagaa agaagaagta aagcctaaga
     3901 tattaataca tacatgctgt gctccttgca gtacgcacac attggaattc atgtctcaat
     3961 atgcagagat cacgttattt ttttcaaatt cgaatattca tcctaaaagt gagtatttgc
     4021 gaagattaca tgaacaaaaa cgatttgtag accaatttaa tgagaaaact gaccatcatg
     4081 ttgcgctaat cgttgatgaa taccgtccaa atgaattcac caaaatggtt ttaacaaacc
     4141 atcttgaaaa cgaaaaagaa ggtggtgctc gctgcagtgc atgcttcaat atgcgattag
     4201 atcttgttgc gcaaaaagcg caagaactag gatacgatta ctttggaagt gccctgacca
     4261 tctcgccaaa gaaaaatgca gctttgatca atcagatcgg aatggatatc caaaagattt
     4321 acgggacaca atacttgcca agtgatttca agaaaaacaa aggctatgaa cgctctttgc
     4381 aaatgtgcaa agagtatgat atttatcgcc aatgttattg tggttgcgtg ttcgctgcca
     4441 agcagcaagg atgcgatctg aaagaaatca atcgggaagc aaaagcttac ttaaaaacat
     4501 caaccgtagt ttttgaggaa aattaaagaa atctttcgga aaaacgtaag aaaaaaataa
     4561 agaatttatg gtagaaataa ggtagactgt ataatttgtc cgtagtaaag taaggaaaca
     4621 agaataaatt tcaatgaagg aagagataag atgcgaaaag tagcacctat tttgaagcca
     4681 atcgctgtat taacaatctc gacgattgtc gcgactacta caggtattgt gaatagttcc
     4741 agctaccgtg caccaagttc aagttctagt actgtagaag aaacaacacc aactacttca
     4801 gtctcgacat ctgaaccaac aacaacggaa agtttgaatg aaaccactac gacaagtaca
     4861 tgggacgata caacaactag ttcgtcgtat gatgagacat atacaactga cagctcaagc
     4921 gaaacaagta atacggtacc agatggcact ccatcggatt caaccaatcc aagtacaagt
     4981 ccggatacaa gtgctagctc cgaaacgata ccaagcagta cgactgtaga tgataacacg
     5041 caaaatgatg atgcagcacg tacagcacag caaaaccaag attcaggaaa tacccaaacg
     5101 tataaccagc aagcaggtga ttctcaatga catttttact agaccttatc aacggggtgc
     5161 ataaattcct tggctattta gacatcagtc ctaaatatct taaccgtgga tatacgattt
     5221 taagtgtgat tcccacgctt tatttattga gaattgttta tggactttgg caaaaccaaa
     5281 actatcttca atttttctta tatggacttg catttctagt gcttgtttat tttactgttt
     5341 taaatgtatt ttattacttt ttagataaaa acacgaaagc cgatgtcact cagctttttg
     5401 tgaagtattt acctgatgaa gcgttcaaca tccagacaga agaatcgata gataatggaa
     5461 attcgatcga taaagtgaat acacaagagg tcatggtttc ttatgacgaa gattatcaat
     5521 tgaaattagc cgagaatatg cggtatttga ttggaaatgg tgagatcaaa acaaatgaac
     5581 tcggtcgcat ggatggattt ctggtcgata gaaatacatt gtatccttat tactatgtca
     5641 agaaaacagg agagaaagag tatgttctaa atattggacg aagctactcg gacatggaaa
     5701 aagtagggac cattcgactt gactcaacag aagacacatt ggaacctgtc ggattgttca
     5761 tcgtcggcgg tgattttgtc aaagacgggt tacgctatca tgagccttat cgtctgaaat
     5821 tgattgcaaa aaaggcacag tcgcaggaag aaccagtggt ttatagtcgt agccaaagaa
     5881 gaagacattc ataatgtgat gaaacgccgt gtatcagtgg ttgcgataaa aacgtttatt
     5941 cggaataagg gggatgtgac acgtcttttg tcacgttcct tttttatata acgagaaaga
     6001 taagacgaac acatcactta ttctttcctt tcatttataa acattgtaga ataaagaaag
     6061 tacaacgaag tattgtgaaa aaacgtaata aataacgagg aaaatacaag taggaggtaa
     6121 gctgtgaaag aatttttgaa aaattggggg attttgatcc ttgtattagc ggctatttta
     6181 ttagctagag tgtacgtgtt tacaccagtt actgtaaacg ggcattcgat ggaccctaca
     6241 ttaagtgatg gacaacgatt gatttcatct aagatctcaa actatgaacg aatggatatt
     6301 attactacaa aggaaccggg ggatgaagaa cggatgattg ttaaacggat catcggtatg
     6361 cctggagata cagtcaagat ggaaaatgac cagttgacca ttaatggcaa aaagtatgat
     6421 gaaccttatc tggatgagtt caaaaaagaa ttttcagaag ataagttaca aggagaatat
     6481 gcttatagct caggattcca agcacaagca gaaagttcat ccacctttac gagtgacttt
     6541 gaatatactg tgccaaaagg aaagtatctt gttctaggcg ataaccgctt gatttcaaaa
     6601 gacagccgaa tgtttgggtt agtcgacaaa gatatgatcc aaggaaaagt tgttttccgt
     6661 tattggcctc tttcagaaat taaaatcatg tgatgaaaaa ataaaaagaa gctgtgacat
     6721 gttttttgtc acagcttctt tttccgaaaa ttccttctta tttcttggag ttgctcactt
     6781 tttttgcaat ctcattttaa gctttttggc tgatggataa gaaaagcgaa gcgtttttgt
     6841 tcataaatag cttttcccca cgatggaagg tatgactagt agcagagaca aagcccgttt
     6901 tttccaatcg attattcaat tcttcttggc taaagccatt atggactttc ggatgactaa
     6961 tttgttcatt cttgtcaaag tctacgatga tcaattgacc gccaggagct aaaatttcat
     7021 aaagttttgt aagaatattc tccgtatcag gaatgtgtaa aagtacaagt gatagaagga
     7081 tgagattagc ttggacagca ggaaattcga cagaagcatc agcgtggatc gttcggacat
     7141 ttttcagatc tgctgcttgg atcttttctt ctgccatttt cagcatagtt tctgaagcat
     7201 cggcaatgat cagttctttg aatctttccg caagcggtaa actaacaagc ccagttccgc
     7261 ctccgtaatc cagcaggact ttttggtggc tgtcgcgcgg tagataagta gttagttctt
     7321 cgcgaataat cttggctaat gcttgacggt caggggaatc ataatggtta gcgatggaat
     7381 caaaaatatt ttttgtcatc aaatagctcc ttacgtaaaa ctttgaaatt ttaacttatt
     7441 atagcacgtg aaaagaaaga aagcatgcta tactcaattc aaaaagggag aatctagaaa
     7501 gaatatgccg atatcgtttg taaaagatag agaggaaaaa ggaaagtgtg tccgagaaat
     7561 attactagac ttgcctgagt ggtttgggct tcctgaatca acggaaaaat atatcgagga
     7621 atcaagtaaa ctacctttat ggtgtgaaaa aagaaaagaa gaatatttag gctttatcac
     7681 tctttcacag acaagtgaag atactgccga aatctattct attgtatggg aataaaaaaa
     7741 gactatcatc accaaggatt aggaacaaaa ctttttaaag aagcagaagg ttacgcagca
     7801 aaacattaca aatatttgca agtgaagact gtagacgagg ggcactactc tatatatgat
     7861 caaactattt gtttctacga atcattagga ttttcaaggt tggaagtttt tccaaattta
     7921 tgggatgaat ggaacccatg tttagttctt gtcaaaaaat tggaacaaaa gtgagcaata
     7981 gatttaatac taatctgtgc ttgatgaaaa ttctcactgt aaaaaatgct gaaaatcaag
     8041 tataatacca taggaacaaa ctggaaggtg aaaaagagca tggcacaact attttttaag
     8101 tatggcgcaa tgaatagcgg caagacgatc gagatcctaa aagtcgcaca taattatgaa
     8161 gaacaaaata agccagttgt cttaatgaca agcggtattg atacaagaga tggtttaggc
     8221 gttgtttcaa gccgaatcgg actgaaacgt aatgcgatgc cgatatttga agaaacagac
     8281 gtatttgacg taattcagaa aatggaaagc aaaccctatt gtgtgctgat cgatgaagcg
     8341 caattcttaa aaaagaaaca tgtcattgct tttacgagaa ttgtggacga attggatatt
     8401 ccggttatgg catttggttt gaaaaatgat tttcgaaacg agttatttga aggatcaaaa
     8461 tatttattgc tttatgcaga caaaatcgaa gaaatgaaaa caatctgttg gttctgccac
     8521 aaaaaagcaa tcatgaattt acattatatt gatggaaacc ctgtttatga aggcgatcaa
     8581 gtgcaaattg gcggaaatga agcttattac cccgtttgtc gaaaacatta ctttcaccca
     8641 tcaatgatta cagaaggaga ataagcatgt acgatcaatt acaagcaatt gaagaccgtt
     8701 atgaagagct tggagaactt ttaagtgacc cagaagtgat ttctgataca aagcgtttta
     8761 tgcagctttc aaaagaagaa gcaaacacgc gcgaaacagt cgaagtttat cgtcgttata
     8821 aagaagtagt acaaggaatc accgatacag aagaactgct tggtgaaaaa ctggatgatg
     8881 aaatggctga actagcaaaa gaagaattat ctgaattaaa aaaagaaaaa gaagtcttag
     8941 aagaaaaaat caagatcttg cttttaccaa aagatccaaa tgacgataaa aatatcatca
     9001 tggaaatccg tggagcagca ggcggagacg aagcagcatt attcgctggc gatcttttca
     9061 atatgtacca aaagtatgct gaagcacaag gctggaaaac agaagtgatg gaagctaacg
     9121 ttaccggaat cggtggatat aaagaagtga tcatgatgat caccggagac aacgtatatt
     9181 cgaaattaaa atatgaaagt ggtgcgcacc gcgtccagcg tgttccttca actgaatcgc
     9241 aagggcgtat ccatacttcc accgcaacag ttgtcgtgat gccagaagct gaagaagtag
     9301 agatcgatat tgaagacaaa gatatccgta cagatatcta tcatgcttca ggtgctggcg
     9361 gacagcacgt caataaaaca gcatctgctg tacgtttgac tcacttacca acagggatcg
     9421 ttgttgctat gcaagatgaa cgttctcaaa tcaagaaccg tgaaaaagca atgaaaatct
     9481 tgcgtgctcg tgtgtacgat aagatccagc aagaagcaca aagcgagtat gatgccaatc
     9541 gaaaatcagc agtcggaact ggggatcgtt cagaacggat ccggacgtat aatttcccgc
     9601 aaaatcgtgt gactgatcat cgtatcggat taactatcca aaaattagat cagattttag
     9661 cagggaaatt agatgagatc gttgatgcat tgatcatgta tgatcaaaca tcaaaactgg
     9721 aagctatgca aaatgggtaa aatgacctat cgagaagtcc tgtcacgggc ttcttctttt
     9781 ttagaggatc atgggaaaga agcatatagt atccaatttt tatttttgga acgaaaacaa
     9841 tggaaaaaac ttgattggct ccttcatatg aacgaagaga tttcagaaga agaacagcgg
     9901 ttgatcgaaa ccgatctgcg acttctgctt gcagatcatc cacctcagta tttgctagga
     9961 tatgcagatt tttatgatca tcggttaaag gtaactgaag caacactgat ccctcgacca
    10021 gaaacagagg agttagtcga atggtgttta gacgagacgc ctggtgtacc gttagaagtg
    10081 atagatattg ggacagggac tggagcaatt gctattagtt tgaaagcagc aagaaaaaat
    10141 tggcatatat ctgctgtgga tctttcagaa gaagcattag aagtagcgaa agaaaatgct
    10201 cagaaagaag gaacgaaaat cagtttttat catggagata cattaggacc agtaatggat
    10261 cagcaatttg atgtgattat ttctaatccg ccgtacatca gtcggaatga atgggaactg
    10321 atggatgaaa gtgtccggtc attcgaacct aaaatggccc tttttgctga gaacgatgga
    10381 ttagctattt atgaaaaaat agcagttgaa gcaccaagcg tactaaaatc aaatggaaag
    10441 atttttttag aaatcggatt tcgccaaggt gaagcagtaa aaaaaatatt tcagcaagca
    10501 tttccagata aaaaagtggc gataaaaaag gatctttttg gcaatgaacg aatgatccaa
    10561 gtttatggtt gaagttgtgg atgaaaaatt ttttatttgt ttttttatta taaaaaaaca
    10621 cttatttgac aaggatgtaa acgacttttt ggtttatcaa caagtttatc cactattgac
    10681 aaatagatta tccacaaaca gtggaaaact tgaggataaa ctcagacaaa ataagaatgt
    10741 atgaaaaagg ggttgtggat aaactgtgga aacgaaacga tatgatgaaa cagaattgca
    10801 agaggcagcc gaagcactga aagaaggaga acttgttgct tttcctaccg aaacagtata
    10861 cggattgggc gccaacgctc ttttaccaaa cgctgtcaag aaagtgtttt ctgtaaaagg
    10921 gagaccgcaa gataatccat taatcgtcca tgtcgcctct tttgaacaag taaaagagta
    10981 tgtggacaac tttcatccgg cagcagagaa gattgtgaag aacttctggc ctggaccatt
    11041 aactttgata tttaagatca aaaaagacac tttgccctca gttgtgacgg gaggattatc
    11101 cacagctgca tttcgtatac cggataacaa aaaaacacta gaagtaatcg aactttccgg
    11161 tgttccgctt gtcggtccaa gtgcaaatac gtcgggaaaa ccaagtccaa cgactgcaga
    11221 tcatgtatat catgatctac aaggaaaaat taccgggatt attgatgatg gagctacgcg
    11281 aattggtgta gaatccactg ttcttgattt aagtgatcca acagccatgc ctatgatttt
    11341 acgaccaggt gcagtaacga aagaacaaat cgaagcagtc atcgaatctc ctgttgctat
    11401 tgatcagcat cttgtaaaag aaaatgaaac accaaaagcc cctgggatga aatacaaaca
    11461 ctactcaccc gatacacgtg ttctgatggt cagagatggc gattggtcaa ctgcagttca
    11521 atgggcaaaa aataagaaga tccgtgcagg tgtcatagca agccccgaaa ttgcggatca
    11581 agtgcggaca gatactgcag ctgtttatat gtataacgac aactcagtag aagcggcggc
    11641 taaaggattg tttgcaggtc taagaggatt agatgaacca acacttggat tagatttgat
    11701 ttttgtacaa gtgtatccag aaactggatt ggggaatgct tacatgaatc gcttgaaaaa
    11761 agcagcagga caaaattatt ttgaaaaatg atcaattggt tattgtcgca ttcttttagt
    11821 atgatacaat gctatcaagt aaatcttcta tcagtgaacg aaaaactaag cgtaactaat
    11881 cgtaatagaa gagatacgaa tatttggcaa tttgtttttg atgggaaaaa taaaattgta
    11941 ggaaaataaa attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt
    12001 ttgaccctga tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg
    12061 agctgattgc ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct
    12121 tgacaaataa atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg
    12181 tcgatatcgt tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg
    12241 cgaacgtcca gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag
    12301 aaccagggga cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat
    12361 caccagtcaa cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa
    12421 cagaagttat tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga
    12481 tcgtagcagg tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg
    12541 cagatgaagt aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag
    12601 caggacttca tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa
    12661 cattgcgtgg ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa
    12721 taaatagtgc ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta
    12781 aagcagtagc attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca
    12841 tcgccaatgc aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt
    12901 caggggcgac agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa
    12961 aagaagcaga gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt
    13021 ttgaaacact aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta
    13081 ctcgcgggtt caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac
    13141 aagcaaaaga tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg
    13201 aaaaatttcc gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca
    13261 atgttgggct tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc
    13321 tctctagtat atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat
    13381 gactgatcgt atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca
    13441 tccgcgtatc tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga
    13501 atcacctgtg aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg
    13561 gggaaaactc aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat
    13621 tttttatatt catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat
    13681 cagagaactt gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc
    13741 agaagcaaaa tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa
    13801 attagccaag tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg
    13861 tggtaacatg gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc
    13921 caagcaattg ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga
    13981 attcggagaa aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac
    14041 gacgaatgaa gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga
    14101 actaaatgat cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga
    14161 aggtgaggct tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt
    14221 ccaaggaatc atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg
    14281 tgcagcaatg gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg
    14341 caggaaaact tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca
    14401 atcacttgat tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa
    14461 tgggcaaatt tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg
    14521 acaaaaactg cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg
    14581 catatgaagt atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg
    14641 aaacaacaca aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag
    14701 gtattggaat ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg
    14761 gtctttatcg agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag
    14821 acattgatgc acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa
    14881 tcatggcaat cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg
    14941 tagcagcacc tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata
    15001 cagcttctct agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg
    15061 cgggtgaccg tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt
    15121 tgtcacatcc ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt
    15181 cgtttgcata taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt
    15241 agttctttgt agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct
    15301 gcagtttcat caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt
    15361 tgcgacttgc cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt
    15421 tccgatggta cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc
    15481 tttcacatca attataccac gttttatgaa aaatatcatg aaattaaact aattttccat
    15541 cggactcttt acaatcagcc aaatagagta tataatttct tttggataag gccagcttag
    15601 ctcagttggt agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg
    15661 gcattaaaag agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt
    15721 tgtttaaagg aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa
    15781 tttaggagaa taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc
    15841 aacttgtaat gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc
    15901 cttgtatcaa aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt
    15961 aatggataaa tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac
    16021 tagaaaaaat agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca
    16081 aaataataaa taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt
    16141 tcaaaatttg ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat
    16201 atttggctat atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt
    16261 taccaaaaac aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa
    16321 tggatggcgc aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc
    16381 cttttttaag ttctattttt tttcctaaaa tgttattcat gataataact cctactattc
    16441 agtttttgaa gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg
    16501 atatttgtat tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg
    16561 taaaaatttt ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg
    16621 ttccgagcgt acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc
    16681 agtatcaaaa aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga
    16741 acagtctaca aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt
    16801 tgaaccagat aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata
    16861 actttccatc aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat
    16921 gctttttgtc aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc
    16981 cattctttga aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat
    17041 tggatggcag attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc
    17101 aaaatcttcg ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac
    17161 tttaggaaaa atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa
    17221 accaacttac attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc
    17281 gctatctctt gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa
    17341 taaaaatctt agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat
    17401 cgttgccata acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta
    17461 gatgtagcgt atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa
    17521 tcgtgatgtc taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt
    17581 taaatccaga gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag
    17641 aaaaaggtca gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag
    17701 caatcagggg agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag
    17761 gaaaatttga aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt
    17821 tataaaaacg agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg
    17881 ctaggttcat ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt
    17941 ttctttatat tgtattttat cctttaatta acaca
//
LOCUS       DAZUKT010000057        17857 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000057 DAZUKT010000000
VERSION     DAZUKT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.058"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACG816_001968"
     CDS             133..636
                     /locus_tag="ACG816_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4920839.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACG816_001969"
     CDS             763..1800
                     /locus_tag="ACG816_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFS4920840.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACG816_001970"
     CDS             1815..3512
                     /locus_tag="ACG816_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFS4920841.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACG816_001971"
     CDS             3505..3966
                     /locus_tag="ACG816_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFS4920842.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACG816_001972"
     CDS             3979..4839
                     /locus_tag="ACG816_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920843.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACG816_001973"
     CDS             4877..5266
                     /locus_tag="ACG816_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4920844.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACG816_001974"
     CDS             5253..7700
                     /locus_tag="ACG816_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4920845.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACG816_001975"
     CDS             7705..9813
                     /locus_tag="ACG816_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4920846.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACG816_001976"
     CDS             9810..10814
                     /locus_tag="ACG816_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4920847.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACG816_001977"
     CDS             10833..11744
                     /locus_tag="ACG816_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4920848.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACG816_001978"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACG816_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACG816_001979"
     CDS             complement(12406..12621)
                     /locus_tag="ACG816_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920849.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACG816_001980"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACG816_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACG816_001981"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACG816_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACG816_001982"
     CDS             complement(15379..16050)
                     /locus_tag="ACG816_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4920850.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACG816_001983"
     CDS             complement(16399..17850)
                     /locus_tag="ACG816_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4920851.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         5818 a         3005 c         3463 g         5571 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgattt
//
LOCUS       DAZUKT010000058        17145 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000058 DAZUKT010000000
VERSION     DAZUKT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.059"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..556)
                     /locus_tag="ACG816_001984"
                     /pseudo
     CDS             complement(270..556)
                     /locus_tag="ACG816_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(733..1911)
                     /locus_tag="ACG816_001985"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="ACG816_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="ACG816_001986"
     CDS             1941..2351
                     /locus_tag="ACG816_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920852.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2432..3052
                     /locus_tag="ACG816_001987"
     CDS             2432..3052
                     /locus_tag="ACG816_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920853.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3753..4037
                     /locus_tag="ACG816_001988"
     CDS             3753..4037
                     /locus_tag="ACG816_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920854.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4040..4957
                     /locus_tag="ACG816_001989"
     CDS             4040..4957
                     /locus_tag="ACG816_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4920855.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4950..5732
                     /locus_tag="ACG816_001990"
     CDS             4950..5732
                     /locus_tag="ACG816_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4920856.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5733..6413
                     /locus_tag="ACG816_001991"
     CDS             5733..6413
                     /locus_tag="ACG816_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4920857.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6406..7302
                     /locus_tag="ACG816_001992"
     CDS             6406..7302
                     /locus_tag="ACG816_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4920858.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            7497..9146
                     /locus_tag="ACG816_001993"
     CDS             7497..9146
                     /locus_tag="ACG816_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4920859.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9740..10675
                     /locus_tag="ACG816_001994"
     CDS             9740..10675
                     /locus_tag="ACG816_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920860.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            10668..11438
                     /locus_tag="ACG816_001995"
     CDS             10668..11438
                     /locus_tag="ACG816_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4920861.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            11854..12837
                     /locus_tag="ACG816_001996"
     CDS             11854..12837
                     /locus_tag="ACG816_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4920862.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            13284..14321
                     /locus_tag="ACG816_001997"
     CDS             13284..14321
                     /locus_tag="ACG816_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4920863.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(14410..14733)
                     /locus_tag="ACG816_001998"
     CDS             complement(14410..14733)
                     /locus_tag="ACG816_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4920864.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(14720..15427)
                     /locus_tag="ACG816_001999"
     CDS             complement(14720..15427)
                     /locus_tag="ACG816_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4920865.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            15513..15854
                     /locus_tag="ACG816_002000"
     CDS             15513..15854
                     /locus_tag="ACG816_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS4920866.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(16025..16873)
                     /locus_tag="ACG816_002001"
     CDS             complement(16025..16873)
                     /locus_tag="ACG816_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4920867.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         5458 a         2915 c         3573 g         5199 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa
     2521 ctggtttttt tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa
     2581 aggaatcggc ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc
     2641 tgaacaaaca tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa
     2701 aaatgtaact gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca
     2761 tgaatgtaat tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg
     2821 ttgcaaggac gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc
     2881 cttatatatg tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa
     2941 aataggtatt gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt
     3001 agaaattttg atagggcaaa aaaataataa cacgaaaata caagtttctt agaaatcatt
     3061 ttttattgag atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag
     3121 gtaatgtttt gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac
     3181 aaagacttat taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg
     3241 tttaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
     3301 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
     3361 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
     3421 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
     3481 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
     3541 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
     3601 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
     3661 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
     3721 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
     3781 ggcttcagcg agaagttcca acgcatatac aaggacgtct gatgtcaacg ataacaacga
     3841 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggcaga
     3901 caactcaacc acgtctgcga gacagtctga tttttcttga aattcaattg gaaaatttac
     3961 actttcttta tacatttctg gctgctttct ttatacccgt caactatact tcacttatca
     4021 ataagaaaga aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat
     4081 aaccaaaaaa tacggtgctg ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg
     4141 aatgatctat ggtttgattg gggagaatgg agcgggtaag tctaccttta tgagaactat
     4201 tatgggtttg atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca
     4261 ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac
     4321 tgcaagagat aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga
     4381 agacctgctg aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc
     4441 tttaggtatg cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt
     4501 gattttggat gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat
     4561 catccaacgt ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga
     4621 tgaattgtca cagatcgcca ctcactacgg tattttgcat gaaggacact tgatcaaaga
     4681 attatcgaaa gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc
     4741 gacggaacaa gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtccaatc
     4801 tagccgtgtt atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt
     4861 ggtagaggca aaagttcctg tggatggtat ccacctagtt gggcaaaaac tggaagatta
     4921 tttcttacaa ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa
     4981 aagtatggaa gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt
     5041 tttattcttg gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa
     5101 cggatacagt catcaacggt gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga
     5161 ttcttctggc tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta
     5221 cgattcgcaa tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc
     5281 tcgttacgct tttgatcaca acacttggcg tggttttgat tacggggcta gccatgattg
     5341 cttacacact tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg
     5401 ttctaaaagt atttcctgta ttgcttctgc tgatattagc gacattatct gttccagtgg
     5461 ctttgacatt catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca
     5521 tgggaacggc cttcgttccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg
     5581 agtggtttac ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc
     5641 aagcaccgaa aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa
     5701 tgtttatttt ccaaaaatct gacattaagt aggtgttcct gctggaaact attttgatta
     5761 tcgaagacga tgaagctgtt cattctttac tggaggaggt tcttgaacag agatacaaac
     5821 ttcttgatgc ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt
     5881 tgatccttct agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc
     5941 gacagacatc gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag
     6001 tttccttgtt ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac
     6061 tgttgttacg aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt
     6121 cgcaacgcat ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga
     6181 atggtgtggc tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac
     6241 atccgaaaaa agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt
     6301 ttgatagtga gaaaacagtg aatatgcata tttccaatct tcgagggaaa ctagcaaagg
     6361 cacatgctgt gtatattcat acggtgtggg gagtggggtt tagatttgat taagtggctg
     6421 ttgcttctcg ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag
     6481 agattaacta agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca
     6541 gtcgaatttc gagaaaagaa tctaatggac ctagtagatg cactgaatac atgggtggat
     6601 gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc
     6661 gtcggccttt ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta
     6721 ctggaacagg tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct
     6781 gttgcaagat tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag
     6841 caaaaagaaa tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc
     6901 tctttttatg aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata
     6961 aatacaactg tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag
     7021 aatttattgc gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggattcattc
     7081 attcgattaa cagtggccaa tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc
     7141 ttccagcgtt tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg
     7201 ctttacctgt caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac
     7261 gggggcatct tctccatctc cgtcaagttg cggagggttt aattggctct aatttacctg
     7321 aagtgaatga caaaaaattg tatctcgttt taaagacatc ttcttttttg gaaggtgttt
     7381 ttattttggt gtgattgata cgaattaggt aacgaagaca gttatttttg tacccccttt
     7441 tcaaatagcg caacagtgct atactaaaac taacaaatca aggaacggag agggatatgg
     7501 gccaaagaga atttatcgtt ttaattatta ttgcagttgt tattttatta gttgttttag
     7561 acattttttc acgtttgaaa gtaaaggaaa cagttcgtag taattgggga aaaattcctt
     7621 atcaaccgag atttgataaa gaagaaagtt tgaaggatgc gtggctcacg gagaaaaaat
     7681 tccgttcatg ggatagtgaa atcgacgatc ttacttggta tgacttggat atgttcgaag
     7741 tatttgaagg gatcaacagt acctattcta gtgtaggttc agaagcgttg tatcagcgtt
     7801 tacgtagctt tgattttgga gaagattatc agttagaaaa actaattgct ttttatcagg
     7861 aaaatcctca actgagagaa agaatccaat atcaattcgc tcgtttagga aaaaaagatc
     7921 ataattttgc aaaacaatat ctagcggatg gaaaaagtaa aaagattgga cgcgtaggtt
     7981 tatttatttt tcttggcttg cttccgctgg tgggtgtagg tttgcttctt ttagggcaag
     8041 tactaggaat ctatctagca atcggtggct tagtattcaa tacggtctat tatctagtga
     8101 agaaacaaac actagaaaca gagctgaata gcatgagtta tttggtttca acgatttcag
     8161 ccgccaatca gttagcaaaa atgacgacac cacttcaaaa agagctgaaa cagcaaacta
     8221 aaccattaag agacatccct aaattaggtt tttcatttcg aatgaagagc gggagtgaag
     8281 cagaattgat ttttgaatat gtcaacatca tgttcatgct gccatttatc tcctatcatt
     8341 tcgttctcac taaattagaa aagcattcaa ctgaagccat caagctgtgg gaattgctgg
     8401 gagaattgga agttgcagca gccgttttga attatcgcac ctatatgccg atcacttgcc
     8461 gaccagagtt tactgagggc ggtgtgacag cgaaagatat ctatcatcca ttgttaaaag
     8521 aagcggtagt taatccagtc aactggcaga agaatacatt agtcacgggt tccaatgcat
     8581 caggaaaatc tacgtatgtc aaaagtatag ccatcagctg tattctagca cagacgatcc
     8641 aaacagcgat tgcagaacag tttaccctgc aacctggaca tgtgttgact tccatggcaa
     8701 tcgaagatga tttatttgaa ggcgacagtt attttgtttc agaaatcaaa tcgatcaaac
     8761 gtttgctgga ccaagtggct tccaaagaac gctgctactg cttcgtagat gagattttaa
     8821 aaggaacaaa tacgatcgaa cggattgctt cttccgcttc agtcgttcgg tggcttgcgg
     8881 agtatccatc tttagcattt gtcgccacac atgatatcga attgacagag attttaaaga
     8941 attattgtga gaatgtccac tttgaagaac aagtaacaga aggaaaagga atcagttttg
     9001 atttcaaatt aaaagaagga ccttcaagga cccgaaatgc gattgccttg ctgaaagttt
     9061 tgaattatcc taaatccata gttgaacagg cccaaaaaga aagtttgttg ttcgatgaac
     9121 agcgtcagtg gtatccgttt gattaacttt atcaaacgaa gcatgatgcg tgggtccaaa
     9181 aagacgatta tcaaaagaag tacattgatg ccaaaaaagt ggcagaattc aatgggaacg
     9241 agtctgctgt ccagatgatc ttggaagatt tagcaaaaga cgatacaaaa taaaccacgc
     9301 tgtcttcctt ctagcagctt ttatcgaaaa aacttagcat cagcgacgag acgcacgttc
     9361 catggcaaag ttctccgtaa aacaaacagc gaaaacagaa attgaaaata actatttcca
     9421 ttttatttat ttcggttaaa ataaaagaaa aaagctttcg ataaaaggac ggaggaaagc
     9481 ttaacatgaa taaaaaagac aagaacctca gttataagga tcttctatat gtcatcgtag
     9541 tagttgctgt cggtactttt atgattttaa aagttccaga gttgtttaga tagttattct
     9601 atcgaagatg atcatgaact agaaaacaca tctcctcttt gttagcaggt gtgtttttgt
     9661 gtggaagcca agttgttttt tcatctgctg tattccatga tacaattaag aatcatgtct
     9721 ataaggagat aggaaataaa tggcaaatgc gcttgaaata aataatttga aaaagatcta
     9781 tggaagtggg gtcgaagcat tgcggggaat cgatctttcg gtgaaagaag gagatttcta
     9841 tgcgctatta gggccaaatg gagctgggaa gtctacaacg atcgggatta ttacttcttt
     9901 agtcaataaa acatctggaa aagtgaatgt cttcggttat gatctggaca aggatctagt
     9961 tcgagcaaag caacagattg gattggttcc acaagagttc aattttaacc cttttgaaac
    10021 ggtccagcaa atcgtcgtga atcaagcagg ctactacggt gtctctcgca aagaagcgat
    10081 gaaacgcagc gaaaaatatt tgaagcagtc caatctttgg gaaaagagaa atgtccgtgc
    10141 acgtatgctt tccgggggaa tgaagcgaag actgatgatt gctagggcat tgatgcatga
    10201 accacgtttg ctgatcttgg atgaaccaac agcaggagtt gacatcgagt tgagacgcga
    10261 gatgtgggag tttctacgag aattgaacga aaatggtaca acgatcattt tgacgacgca
    10321 ttatctagaa gaagctgaaa tgctttgtcg caacatcgga atcatccaat cgggtgaact
    10381 gatcgagaat acaagtatga aagagcttct gtcaaaactg caatttgaaa catttatctt
    10441 tgatttagcg ccatatgata ccaaacctgt gattgaagga tatgcttgta catttgaaga
    10501 tgagcagaca atcgctgtcg aagttgaacg aaatcaaggg gtgaacggaa tcttcgatca
    10561 gcttacaaga caaggaatca aagttctttc tatgaggaat aagtcgaatc gtttagaaga
    10621 actgttcttg aaaatcacag aagaaaataa tcatacggag gagaaaaatg tttaatcttt
    10681 attttactgc attaaaaagt ttagctatca aggaaacgaa ccgctatttg cgtatatggg
    10741 tccaaacact ggttccgcca gtgatcacga catccttgta ttttgttatt ttcggaaaaa
    10801 tgatcggtgg gcggatcggt gacatgggtg gtttttcata tatggaattc atcgttcctg
    10861 gtttgattat gatgtctgct atcactagtt cgtatgccaa tgtttcttcg tccttcttct
    10921 ctcaaaaatt tcagaaaaat atcgaagaaa tcttggtagc acctgttcct acgcatgtga
    10981 ttatttgggg ctttgtgatt ggtggtgtgg gtcgcagtat tctcgtcggc tctctcgtaa
    11041 cgatcatttc cttgttcttt gtaccacttc atgtgtattc atggttcatg gtcatcatta
    11101 cattattgat gacggcaatc ttgttttcat tagcaggact gctcaacggg atttttgctc
    11161 agtcttatga tgatgtatcg atcgtgccta catttgtctt gcagccgtta acttatctag
    11221 gtggcgtttt ctatgccatc tctatgttac cgcccttttg gcaagctgtt tcaaaagtca
    11281 atccgatcgt ttatatgatc tccggtttcc gttacggatt ccttggaacg atcgatgtgc
    11341 cagtagcaac atcgatgatc gtacttgtgt tattcatcgt tgttttgtac gccgttactt
    11401 ggtatttgat caataaagga agaggattga gaagttagaa aagaggttgt gaaatcagcg
    11461 ttttgacctg agtattagag aaacaaacgg gaaaatagtt cctcatattt tgttgttttt
    11521 tgagcgaata ccgagggtca gctgattgaa caccgtttag tggagaggtt gtgaaaaagc
    11581 tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg
    11641 gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttattccgga aaatggactg
    11701 tgataagatt tttgtcacag tctttttttt gctcaatcag aaacatcttt taagctgttc
    11761 actttgcttc tttttttgtt tcctagtata atgacaatgt ttttgagaaa gattgcgact
    11821 tcactgcttg atagaatgga gagtaacaaa taaatgacta ctaatttttg gaaagagctg
    11881 ccaaagcctt tttttgtttt agcaccaatg gaagatgtaa cagatgttgt atttcgccat
    11941 gtcgtaaagc atgcagcagc accggatgtc ttctttactg agtttacaaa ctcagacagt
    12001 ttctgtcatc cagatggaaa agacagtgta cgaggaagat taacatttac agaagatgaa
    12061 cagccgatgg ttgcccatat ttggggagac aagccagaat tttttagaga aatgagcatt
    12121 gctatggcag aaatgggctt ccaaggaatc gatatcaaca tgggctgccc agtccctaat
    12181 gtagcagaac gtggaaaagg cagtggcttg atccttcgtc cagaagtagc ggcacaattg
    12241 attgaagcag ctaaagctgg cggtttgcct gtcagcgtca agacgcgtat cggctatacg
    12301 gaaatgtctg agatggaaga atggatcagt cacctacttc atcaagatat agccaatctt
    12361 tctgtccatt tgcgtacgcg taaagagatg agtaaagtag atgcacattg ggaagtcatt
    12421 cctaaaatca ttgctttacg tgaccaaatc gctccacaaa cactaatcac gatcaatgga
    12481 gatatccccg atcgccagac aggagaagaa ttggccgaga aatatggcgt tgatggcatc
    12541 atgattggcc gagggatttt caagaatcct tatgcatttg aaaaagaccc gagggaacat
    12601 acagaaaaag aactgatcgg actacttcgt ctgcaactag atctgcaaga tcattattca
    12661 gaaatcatcc cgcgttccat cacaggacta catcgtttct ttaaaatcta tatcaaagga
    12721 tttccaggag caaatgactt gcgagttcag ttgatgaata cgaaatctac tgatgaagta
    12781 cgagagatct tagagacctt tgaaaaagaa catccaacgc ttttcagtga acaatgattt
    12841 cttgcttgaa caccgtgaat tagaggtcgt gaaaaagctg tcctgtatca agtaataaga
    12901 acagccaaac aaaaatagct cttcatattt tgtcgttttt tgagctaata ccgagggtca
    12961 gttgattgaa caccgtttat atcagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    13021 ggagcaattt caaaaaacag cttctcatgt ttgatgaata ttttgacttg tgaccgaaga
    13081 gttggtagga gaacaccgtt tataagaggt tgtgacaaaa gtcttgtcgc aacctctttt
    13141 ttaaccaaaa aagtgcaaat agtttacttt aagtaagaga ataattatga tgaataaaaa
    13201 aggtggaaca aatagcatac tatatatatt gaaatgaaca ctgtatggat gctccaactt
    13261 gatgaagcta aggaggaatt cgtatgtcag aaaaggaatt aatgaaggta gtcggttttt
    13321 atgaagggtt gccgatcgat gatccaaaaa gctttattga tgaaaaagaa cagatacctg
    13381 tacccagttc acgggatctt ctggtaaaag tcaacgcagt atctgttaat cctgtcgata
    13441 cgaaacttcg ccaagataac gggataagaa atgctttacg tatcttaggt tttgatggag
    13501 tcgggaaagt tgttgctgta ggtgacgaag tgcaaaaatt ttctgtcggc gatcgcgttt
    13561 tttacgcagg tacaacaaca agagcaggaa gcaatcaaga ataccaacta gttgacgaac
    13621 gtattgtcgc tcttgcacca aaaaatcttt cagatgaaga agctgcagcc ttgccgttga
    13681 cctcattgac cgcttatgaa ttgttatttg aaaaatttgg tctaactcca gaagaaaatg
    13741 ccaatcgcgg gaaaaaaatc ttagtgatca acggatccgg gggcgtcggt tcgattttga
    13801 atcaactagc gcattgggct ggcctagaag tctacgcgac tgcaagtccg aagaattttg
    13861 aatggctgaa aaaaacaggt gtagactatc caatcgatta tcatcacgac ttaagtaaaa
    13921 gttttcgtga attaggaatc gatcaagtag agtatgtcgc tgtattattc gatattaccc
    13981 gctactttga tcaaatcaag caccttgtcc gaccatttgg acatgtggga acgatcgtag
    14041 ggatcaaaga gccgttagat ataggtgttt ggaaaaacca atctatcagc ttcgactggg
    14101 agtatatgtt cgctaaaaca gactataact atgaaatcga aacgcaagga gaagctttag
    14161 cgcacattgc tgctttagca aatgaaggga ttatccgcag tacattgagc aaggtctatt
    14221 cagatgggat gaatgcgaag aacttgaaac aagcaaccaa agacgtagaa acaggacata
    14281 tgttaggaaa agtcgttgtt agtggtccgt tcaacggata agcaagaata cgccgatttt
    14341 tttcttattt gactttgata aaaacaagag cgcatgacag taatttgcca taagctcttg
    14401 tttttttatt tataataagc gaatcaacga aagggaaaga attcctgcca agacgatcac
    14461 caataagctt tttgaaagaa tagcagctaa aagtgtagga actgaagcca aagcattttc
    14521 gagattgatc tgcggcagat gtccgatatt ttgggtaaat agactgctga accataaagc
    14581 agacataatg acgatcggga caaaacttaa atactcaagc aacggttgcg gtaaatcgaa
    14641 ttttttcagt aagacaaatg gtaggaccct agaaagccaa gttgctaaag tacaacctaa
    14701 aatagtcagc aatgtaaact caaaagaagg catgtttgat ccacaccccc aatccgcacc
    14761 caatcagtgt tacaactaca atcactaagt tgcttgggat aaaaatcagt ccgacatata
    14821 ccagaatcaa agtgagacca atcaggatca actgtaatcg tttagaggtg tttctatcag
    14881 aaatgacttg aagataaagt aacccgataa acatagcgac gatggcgaaa tccaaaccaa
    14941 actttttcgg atcagtaata aaattcccta aaagtgcgcc aacaagagaa gaacccaccc
    15001 acgtcaaata agaaatccaa ttagctgtat tcatccaacg aaagtttaac ttttgtccag
    15061 tatagttcaa tttattcatt cccagtgcga aactttcatc tgtcagcaat gtgccaatca
    15121 gcagattttg aagcagccga tttttcttga aataaggagc aatcgtcata ctcatcagaa
    15181 tcattcggga attgactaga aaggtcgaga aaacgatgga aagcaatggg ctgtgactag
    15241 caagcatgct gactgtgata aattgggcag aaccagcata gaccagcagg gacatcaacg
    15301 tgacgactag tggatgaaat ccggcagctt tcccaacgat accaaatgca agtccaatac
    15361 cgatataacc gaaaacggta ggtaacgtgt ctttgatcgc agtttttata tctaactttt
    15421 catccaataa caaaactcct ttagcacgac ataaattttc ataattgtcg ttaaacttgt
    15481 gaatattcta aatttttaat gagcatttgt caatggtggg attgaaacgt aaagaactag
    15541 cgggtcgcag atttacacgt attgtttcgg tgggaggagg cgcgaaaaat cttgcatggc
    15601 tacaaatcca agctgatatt ttcgatgcaa cggtctattg tttaagtgtg gaggaaggac
    15661 ctgcattagg cgcaacaatg attgctgcac tagggttagg gtggttcgac acacctgaaa
    15721 gttgttgtca gaattttatc caatatacag atgcagtgga acctctctta caaaatgtag
    15781 aaaagtatca taaaatctat caacaatgcc ggaaaatcta tccagcaacg aaggaaattt
    15841 gtcatgactt ataaaacaaa acgcgtacct aatagtcaaa tgactaatat tgggtacgcg
    15901 ttaagtcttc tttacttcat cctttgcatg attcgtgttt ttaaagcaag gtctgctgta
    15961 tttcttgtgg atttaggatg gttgttatcg tttacttgct atttttcatt gaaatcgtct
    16021 ttctttaccg tttctttagt tttttatcta tttttttctt ttggttagaa aattcttttt
    16081 cccgaaagtc ttctttcagg atttttatag agtagcgttt tgaataagag gcgtcagtat
    16141 gatcgacttg gcagacgaac caagagaaca tttgcctttt ttctgcatga ggtgcttctg
    16201 ctaataattc tttccataaa taataacagt cctctatcag aagaagggtt cgttcatcct
    16261 caggctccca atagaatgtt tcgagagtag caatcacttc attgatcaat tcgaatgcgg
    16321 gaagataggc attttgctga ataagcgatt caactgcttc ttggatgaac tttcgcatct
    16381 ccatgatgaa cttcgctgtt ttcctgtttt tgtttttctt gataatatga tcgtatttat
    16441 ggatgatagt tgttaattta tctgaataat ttattgcatg aaatggcatc ttaactttga
    16501 agcgttcgat aaagtgcgga tgttctataa gaatctctgt taaaagctcc cgtacaagag
    16561 aggaactagc ggaggaaaca agggacgaga gttcggaaag tgagtagttg gattttttta
    16621 ttggacgttc ttcgtaagcg aataagacag cagccatgtg tttgcatttt tttccttcta
    16681 tggcatatag acaatcacaa gtcatgttgg tcttttcatc ccaagtgatc actaccttat
    16741 aatcttccgt gccagaaact gttgcggaca agtacttgct tgtatagttc aaatgacgga
    16801 caaaaccgtg cagatgatag ctgtaaccgc gttccaaaac gttttcagga aaatactcaa
    16861 gccaattatt catctagttc tcctttcatg aaacacttat ttatatttag tataaagaaa
    16921 cgtttaagaa aaaagtagct gattttacat atttgataat cacttttgct cgaattgtca
    16981 aaaattcatt ctccttttct tttcttagag aatgtgagaa aaagtaaaag aatagcgtgt
    17041 tttaaagatg gaactttttt tgttttttat ttatctgaca aaaatgaagt gattttgtta
    17101 gaaataaata attgttccaa ttgataaaag aggtatgatg tatcg
//
LOCUS       DAZUKT010000059        17007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000059 DAZUKT010000000
VERSION     DAZUKT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.060"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..126)
                     /locus_tag="ACG816_002002"
     CDS             complement(<1..126)
                     /locus_tag="ACG816_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS4920868.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="ACG816_002003"
     CDS             complement(230..541)
                     /locus_tag="ACG816_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4920869.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="ACG816_002004"
     CDS             complement(543..740)
                     /locus_tag="ACG816_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4920870.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="ACG816_002005"
     CDS             complement(1134..2861)
                     /locus_tag="ACG816_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4920871.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="ACG816_002006"
     CDS             complement(2854..4047)
                     /locus_tag="ACG816_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4920872.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="ACG816_002007"
     CDS             complement(4234..4878)
                     /locus_tag="ACG816_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4920873.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="ACG816_002008"
     CDS             complement(4972..5106)
                     /locus_tag="ACG816_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4920874.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="ACG816_002009"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="ACG816_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4920875.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="ACG816_002010"
     CDS             complement(7237..7710)
                     /locus_tag="ACG816_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4920876.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="ACG816_002011"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="ACG816_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4920877.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="ACG816_002012"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="ACG816_002012"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4920878.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="ACG816_002013"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="ACG816_002013"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4920879.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="ACG816_002014"
     CDS             complement(11757..12896)
                     /locus_tag="ACG816_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4920880.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="ACG816_002015"
     CDS             complement(13205..13918)
                     /locus_tag="ACG816_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4920881.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="ACG816_002016"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="ACG816_002016"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4920882.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="ACG816_002017"
     CDS             15107..16639
                     /locus_tag="ACG816_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4920883.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="ACG816_002018"
     CDS             complement(16830..>17007)
                     /locus_tag="ACG816_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4920884.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DAZUKT010000060        15998 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000060 DAZUKT010000000
VERSION     DAZUKT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.061"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..282)
                     /locus_tag="ACG816_002019"
     CDS             complement(<1..282)
                     /locus_tag="ACG816_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920885.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
     gene            complement(384..737)
                     /gene="tnpB"
                     /locus_tag="ACG816_002020"
     CDS             complement(384..737)
                     /gene="tnpB"
                     /locus_tag="ACG816_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4920886.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(727..921)
                     /locus_tag="ACG816_002021"
     CDS             complement(727..921)
                     /locus_tag="ACG816_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920887.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            1004..1612
                     /locus_tag="ACG816_002022"
                     /pseudo
     CDS             1004..1612
                     /locus_tag="ACG816_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1632..2660
                     /locus_tag="ACG816_002023"
     CDS             1632..2660
                     /locus_tag="ACG816_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4920888.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            2657..3415
                     /locus_tag="ACG816_002024"
     CDS             2657..3415
                     /locus_tag="ACG816_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4920889.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(3501..4241)
                     /locus_tag="ACG816_002025"
     CDS             complement(3501..4241)
                     /locus_tag="ACG816_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4920890.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            4302..5555
                     /locus_tag="ACG816_002026"
     CDS             4302..5555
                     /locus_tag="ACG816_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4920891.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            5850..6104
                     /locus_tag="ACG816_002027"
                     /pseudo
     CDS             5850..6104
                     /locus_tag="ACG816_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            6226..6552
                     /locus_tag="ACG816_002028"
     CDS             6226..6552
                     /locus_tag="ACG816_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4920892.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            6620..7333
                     /locus_tag="ACG816_002029"
     CDS             6620..7333
                     /locus_tag="ACG816_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4920893.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            7643..8495
                     /gene="licT"
                     /locus_tag="ACG816_002030"
                     /pseudo
     CDS             7643..8495
                     /gene="licT"
                     /locus_tag="ACG816_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            8616..10481
                     /locus_tag="ACG816_002031"
     CDS             8616..10481
                     /locus_tag="ACG816_002031"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4920894.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            10495..11938
                     /locus_tag="ACG816_002032"
                     /pseudo
     CDS             10495..11938
                     /locus_tag="ACG816_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            12046..12747
                     /locus_tag="ACG816_002033"
     CDS             12046..12747
                     /locus_tag="ACG816_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4920895.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            12873..13385
                     /locus_tag="ACG816_002034"
                     /pseudo
     CDS             12873..13385
                     /locus_tag="ACG816_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(13783..13992)
                     /locus_tag="ACG816_002035"
     CDS             complement(13783..13992)
                     /locus_tag="ACG816_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS4920896.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            14233..14808
                     /locus_tag="ACG816_002036"
     CDS             14233..14808
                     /locus_tag="ACG816_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4920897.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            14808..15248
                     /locus_tag="ACG816_002037"
     CDS             14808..15248
                     /locus_tag="ACG816_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4920898.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            15264..15965
                     /locus_tag="ACG816_002038"
     CDS             15264..15965
                     /locus_tag="ACG816_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4920899.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5351 a         2689 c         3338 g         4620 t
ORIGIN      
        1 tttcgttcct acaactcgtt gatgtccttt tactggaata atggtggcag ctgcttctgg
       61 aacacttaca gaaatttctt catcactaaa aagattcaat tgcccattcg tttcctcaaa
      121 gagagtctct ttagagcgtc caaataattt gttggttaaa taattgacct tttcattcaa
      181 tagttggatt tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat tggttttatg
      241 ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc accaccttga
      301 atcgaactga ttttgttaca gaataccaca agaaacgcac aagaaatagg ggttcaacac
      361 attttttgtg ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga
      421 ggttgatcga tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga
      481 acttcgtctt tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga
      541 agaaaaccat caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat
      601 aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga
      661 ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga
      721 agtaaatcat aaagcattgg acaacagttc tttcaaaata gcttgaagaa tataattgtt
      781 aataccgtta tagatcaaaa cttcagaacc attctctctt ttgatgcgca tcgctaactt
      841 ggcagccgcg ggtttacctt tttctggtgc actcttttcg tttcctaatt ggacaggaac
      901 aatctcattt aatttataca tagaaaacct ccagacttct tgatagctca agtatatctt
      961 gaggtgcatt tgttagtaag gtactgttct atggggcgct tacccagaaa atcaactttt
     1021 tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagctg ctgctaaggg
     1081 tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa gtgatacgcc
     1141 agttgtttat catgatgccc cgccttttgc acctgctacg aagattgtag tagaaggtgg
     1201 tctctcattt cttgcccctt ccaaactcga ttgtggtcag atcaaagcaa ctgggttgac
     1261 gcaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg atcagcgaga
     1321 aacaaaaagt ccatggatct taacgataaa attatctcag cctttcacaa ccgatgcagg
     1381 attttctatg caggatgtcc tatatttcaa gaaagctgag gagtatcaat tagtgaatga
     1441 agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaaacag attattcaga
     1501 atatttaaat aatggtcggg cgtttaaact tgtcttaacc aaagaaaatc agatagctgg
     1561 taattactca gcagagatga catggacatt agcagatgcg ccgacagaat aaagatcaag
     1621 gagaatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc tctttacttc
     1681 tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc gaattcaatt
     1741 ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca tatataaatg
     1801 taaggatgga gcctgggaaa acacaggaaa tcccctacac ggtctttaac cgaacagata
     1861 aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac ggtgtgatcg
     1921 aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg aaaaacttag
     1981 caaaaacagt gggagaaata actgtccctc ctcaaagtgc ggtcactgag acactttcac
     2041 tgaaagctcc agaaaaagag ctggctggtg tggtcgcagg aggaatcacg tttcaagaaa
     2101 aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt tctcaagtga
     2161 aagcaatctt gattcgaaat agcgaaaaag agtcttcccc aaatttgaaa ttaaaaaaaa
     2221 cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa aatccaaatg
     2281 cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa acaaaagagc
     2341 cttatctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat tttgatttac
     2401 ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt gtgaagggtg
     2461 cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat gatgcagcgg
     2521 accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt gactggcgat
     2581 ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt attttaaaag
     2641 aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag ctggaagtct
     2701 attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta cagaaggaac
     2761 gatttctgta acaaaaccag atgccgcaaa tcctgaagac gttgttccag ttcctcctgt
     2821 tgatccaaat gatcccgata cgccgtttac acctaacgaa ccaaatcagg gaacatccgg
     2881 attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt taggtacagc
     2941 aaaaacagta tatgctgcat tacaaaacgg aacggacagt acagggacag caacacaagt
     3001 acagaatttc gtacaagtaa cggataaaag cggaatttat gccggttgga aactatcagt
     3061 caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg gttcaaaggt
     3121 gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa cgccatctac
     3181 tatttctggt actggcgctg aaggaattga gatcccagca gatcaggcag tagatctagt
     3241 aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag agaatgtgga
     3301 acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgccg tagacaacta
     3361 cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagggaaag cgtaaaatca
     3421 aatcaatgac ttcagggagt ggagccgatt caaggccaac tccctgaaat ctgctatgct
     3481 tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg gaaacgttcc
     3541 tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccattgaa aaattctaac
     3601 acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta agattttaat
     3661 agtaaaaagt aagcatactt ttccaactct gtcttcaatt gttttaaaga agtattcact
     3721 aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt gatactttca
     3781 atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc aataaagatt
     3841 ttatcgacat ttacgatttg ctgttctttc caattgatct gttctagcac ttgacgaatt
     3901 tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt ccgattgatg
     3961 atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg tgcatttttt
     4021 ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc gatatcgata
     4081 aagaaaacat cgtgattatc aatagtaccc aactggatca actgattgat ttgagagata
     4141 gaagtgagct gcacgatatt ctcgatatga tgattgacca attctttgat gatccattcg
     4201 ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct cctattaaaa
     4261 aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca acagtgacac
     4321 tttatttgac ctttactttg attttagatt taggtatcta tttattcttt tcgaaacaac
     4381 tgctagtaaa aaacagcttt ggttttatag ctttgatggt ggtgacactt ttatttccta
     4441 tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt ttttatttta
     4501 ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct atcactggac
     4561 tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat cttggaacag
     4621 caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa agtatagaca
     4681 aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat tttggaggta
     4741 tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt cacttccgct
     4801 catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt tctttgtttt
     4861 tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa tcgcttcaac
     4921 tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat gattataaaa
     4981 gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg ttggaatttt
     5041 tagaaatctt agaagatcat tctgcagcta tttttgaaaa accgcaaata caacagctaa
     5101 caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa aaagcagaat
     5161 cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc ccgattgatc
     5221 tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat catcgtgtag
     5281 ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatta cgttttagta
     5341 tagcgaacaa ggcaaatccg acgaatttga cgctatcgga gatggtcaaa agaggaaaaa
     5401 cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg gatgtttacg
     5461 aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt gtagaaatgt
     5521 atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga taagcttgag
     5581 gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac agaagcgtga
     5641 tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag ttgacacttc
     5701 cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa gaagtgaaaa
     5761 gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa agggaagagc
     5821 aatatcgctt attttaggcg tggagaagca agaaaatccc tacgatcaca caaaaaatgc
     5881 tgacccagca gttaagagag ttagaacaag atggcattgt ccatcgaaaa gtctataacc
     5941 aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta agagagattc
     6001 tagtagcgat gtctgtttgg ggagaagaac gtatcgaaaa acaaaaagcc tcaggggaag
     6061 aagtcacatt actgcataca catggtggct acctaaatta ctgatttcta tgacatttat
     6121 tttaaataaa agaaaacagg agttttttta aaagcatcat ctatttttta tgtaaaaaaa
     6181 tggtatgatt aatcaagtaa gaaatgattg gaagaaggca agcaaatgaa tagcatggaa
     6241 aaagttaacc agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg
     6301 gctatttcta tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg
     6361 gaagaagtaa aagaaacaca attagaacga atcaaagaag aactggcgga tgtattgatc
     6421 tattcataca tgatggcaga caaccttcag ctggatctag atgagatcat tgaagaaaaa
     6481 ttgatcaaaa ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac
     6541 acagagtttt agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat
     6601 gagtctggag ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat
     6661 cctcttgttc ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga
     6721 ttatcttgtc agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct
     6781 gaaaaagaat aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac
     6841 agaaggggtc atgaggtcgc agatgagagg aacagatcta ccagtgattg ctagtatcgc
     6901 agtcccttca gtttcaatca atttaccgat tttcaaaggc ttggataata cgtcactact
     6961 ttacggagca ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc
     7021 aagccatcgt gcaacgaacc ctgaattgtt gtttacaccg ctggaaaatt tagagatggg
     7081 tgccaagatc tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga
     7141 aaaagttgct cctacagata cccaattact gaatgaagtc gaggaaaaag aaattgtcac
     7201 actaattact tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga
     7261 aagtgttaca ccgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac
     7321 aaaaacattt taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat
     7381 gaatcgataa ggaaaaacca atttgctgaa aaaaagagga acgcaaagag gtgcatctct
     7441 gatttggttg ttttctagat agacttgtgt tccagttttt ttatttttta tatgaaaagg
     7501 cttgcataaa taaaaattcg atggtatgat gattttgaat aagaaatgga ttgttactgt
     7561 acggcaggca aaaccagaat tcagctaaga aacagtaggc gaattctagg tttttttatt
     7621 tttcttgaga gggcgaagca aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat
     7681 ttcgaaaaat ggattcgggg aagaagtcgt ttgtatggga aaaggactag cttttcaaaa
     7741 aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttatt ttaagagatt
     7801 cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag gagatcgaat
     7861 gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt tcgcctaata
     7921 tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa gaaaatatcc
     7981 aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa tatcaagcag
     8041 cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct gaggatgaag
     8101 ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac atgcaagtga
     8161 cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa ttcttcggca
     8221 tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg cagttttttg
     8281 cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc tacgcattag
     8341 ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag tatcttcgac
     8401 aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt catattcaaa
     8461 gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat ggcaggcaaa
     8521 acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa tctgggtttt
     8581 tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca gttggtcgtc
     8641 gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat tgtgctacgc
     8701 ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta aaagaagacc
     8761 cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt ggcagtaatg
     8821 tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg gaggatggaa
     8881 aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt atatttacac
     8941 cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca ttggcaacgg
     9001 tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct gcagcagatg
     9061 gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag ttcaaagcca
     9121 accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc acagcagttg
     9181 ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca ccatctggtt
     9241 atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa ttagagccat
     9301 tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt gtcgttttag
     9361 tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt gccggtaatg
     9421 ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca ggtctaatca
     9481 tgggaagttt atggcaagta ttcgtgatgt tcggcatgca ttggggattc gtaccgatca
     9541 tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt cttccagcca
     9601 tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat acgaaacttc
     9661 gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa ccaactgtgt
     9721 atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct ggaggtattg
     9781 gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg gtcagcttat
     9841 tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg acagtagcag
     9901 taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt ttaggatttg
     9961 acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga gaaccgatta
    10021 cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca ttgagtgaag
    10081 taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt gatccagaag
    10141 taggagaact agttgcacct gcagacggag agatcacgac tatttttcca actggacatg
    10201 ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg atggatacag
    10261 tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc gtcaaagcag
    10321 gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat agcgtaatca
    10381 ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta gaccaaaaag
    10441 agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat caggatgcaa
    10501 tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc aattagaagg
    10561 ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg gaggtaagca
    10621 gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag aaaaatatat
    10681 ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc ttgccttgtt
    10741 tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa tttttccaaa
    10801 tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc tgattgatga
    10861 gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa tgcctcttca
    10921 tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct atgaacgatt
    10981 tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga ctttcaatga
    11041 aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa gcaatggagc
    11101 aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa gtagcaaagc
    11161 cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga ttatctatgc
    11221 aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga tccaaaacca
    11281 agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg catacacagc
    11341 gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg aaaaagactt
    11401 ccagttgcta aaagaatatc cagtggacta catcggattt agttattata tgtctacggc
    11461 aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg gaggagtaaa
    11521 aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag ggctgagaat
    11581 cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg aaaacggtct
    11641 tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc ggatcgatta
    11701 tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag atttgatggg
    11761 ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa tgagtaaacg
    11821 ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga atcgatcaaa
    11881 gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa acttgtaatt
    11941 aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct ttttttattt
    12001 tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa aaggacacgt
    12061 gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg aaaatatcca
    12121 gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt cagcaaaatc
    12181 actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa gaaaagtggt
    12241 cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc ggcaagcttt
    12301 acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga tttgcaattg
    12361 gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc tgtttgtttt
    12421 aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta tcttccacaa
    12481 gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg tttgctgacg
    12541 aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt tttaacagaa
    12601 gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat acggaaatcg
    12661 tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac ggagctgcat
    12721 ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa gctgccctgc
    12781 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct
    12841 tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa gggggtgtgg
    12901 cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct tcgatggtaa
    12961 ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa aatatactca
    13021 atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt cagatcacag
    13081 agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca acgggagtcg
    13141 gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt attccggcat
    13201 cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt gcattgttag
    13261 ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta gatccgaatg
    13321 cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat ttgacaatta
    13381 gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct tgagacagga
    13441 atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt ttaggagcga
    13501 tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa aaatgagaac
    13561 ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac aaaaaaactt
    13621 tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga aattattctt
    13681 catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca ggcaggcgca
    13741 acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga tatttttaca
    13801 aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa caaaaatgat
    13861 caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat ttatcagtga
    13921 aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta tttgttgttt
    13981 aaaataatac acgttattgg catataggac gaaatcccag tatcttctat aagtaagtca
    14041 tttcatatcg atgaaaacac gttatttatt tttgttttat ctataaaatg gctgaaaaat
    14101 gatttttttc taaatactat atatagtggc gtctatccta tataccacta aaaaactaca
    14161 aaatctagtc ttttatttta aaaaaacacg tgtagaatga agatagactt ttgagttagg
    14221 gagtgttttt ttatgaaact tactgttttt tgcggttccc gttttggtaa taaagaaagt
    14281 tacaaattta ttgcgcaaac gttaggaaaa tacatggcac aagaagaaat cgaactggtt
    14341 tacggtggtt catactccgg gattatggga gtgattagta aaacagtact ggaaaataac
    14401 ggaaaagtca caggtatcta tccgaatggg ctttttgaaa ctgaattgcc tggaaaagat
    14461 gttactacat ttattcctac agaaacgatc gatgaaagaa aggtattatt atttgaaaag
    14521 ggagatgctg tcctcgtttt ccctggaggt ctaggtacat tggaggaatt ctcacaactt
    14581 ctttcttgga tagcaatcgg tctgacgccc gacaagccaa tcggaatatt gaatattggc
    14641 ggttattaca gcggcttgca acaattatta gaaacttttg caaaagagga ttttatggat
    14701 aaaaaatggt tggatcaagt aatcttttcc aacaatccat tggaactggt caatgaatta
    14761 cgagcagtat ctattaaaaa tcagttatta ttgaaggagg caaaataatg agtcaaatat
    14821 atttagcagg accatttttt tcagaggagc aaatcgatcg agtaagtcgt atcgaaaaag
    14881 cgttggagga aaacaaaaca gtcacaagtt tctactctcc acgtcatcac caagaaagca
    14941 attatgaact attcagcgct ggatgggcgc aggaagtata cgaaaaagat atggaagaac
    15001 tgacaaatgc agaatttgtt gttgctatcc ttgactttga gcatcaaaca atcgatccag
    15061 gaacggctta cgaattaggt gtggcaacta tgttgaaaaa accgatgatc attgtacaag
    15121 aagagacggt acctacgaac ttgatgatta cacaaagtct ccatacgtat ttaaaatcag
    15181 accaagctgt acgtgaatat gattttgaaa cattgcctgt tgaaacttac gtaggcgagt
    15241 acttatagaa gaggaagggt tgtatgtcag tgattttgat ttcaggaacg attggtgcag
    15301 gtaagagcag tttgacggat atgcttgcaa aagagattga ttctaaacca ttttatgaaa
    15361 atgtcgaaga taacgaagta ttgccgctct tttattcaaa tcctgagcag tacgcttttt
    15421 tattgcaaat cttctttttg aacaagcgat ttttagcaat gaaaaatgct ttagtgaacg
    15481 atgacaatgt gctagaccga tccatctatg aagatagcct gttgttccat ctaaacgctg
    15541 atttaggtcg ggtaacggat atcgaagtac agcagtacga tagcttatta gatacgatgc
    15601 taaacgagtt agatgatgtt gctccaaaaa aacggcctga tttgatggtc catatcaaag
    15661 tttctttgga cacgatgcta gaacgtatca aaaaacgtgg gcgtgactat gaacagctag
    15721 aaagcgatga gacgttatat acctattatg aaacgttgaa cacccgctat aatcaatggt
    15781 atgaagattt tgatgtatgt cctaaaatac aagtggatgg ggataaattc gattttgtag
    15841 aaaatccaga agatgcaaaa aaggtgatcc gtcagatcca agaaaaatta gctgaattgg
    15901 aaggaaagcc tgctggaaaa tattttacgg ataaccagca tgcaaaaggt caagaattag
    15961 gctaaaaata aaaaaccaaa cgacgggaaa gaaaaatt
//
LOCUS       DAZUKT010000061        15423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000061 DAZUKT010000000
VERSION     DAZUKT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.062"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..697
                     /locus_tag="ACG816_002039"
     CDS             290..697
                     /locus_tag="ACG816_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920900.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="ACG816_002040"
     CDS             711..1112
                     /locus_tag="ACG816_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920901.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..1485
                     /locus_tag="ACG816_002041"
     CDS             1114..1485
                     /locus_tag="ACG816_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920902.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1521..1826)
                     /locus_tag="ACG816_002042"
     CDS             complement(1521..1826)
                     /locus_tag="ACG816_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4920903.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2353..2425)
                     /locus_tag="ACG816_002043"
     tRNA            complement(2353..2425)
                     /locus_tag="ACG816_002043"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2390..2392),aa:Lys,seq:ctt)
     gene            2806..3375
                     /locus_tag="ACG816_002044"
     CDS             2806..3375
                     /locus_tag="ACG816_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4920904.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3362..4222
                     /locus_tag="ACG816_002045"
     CDS             3362..4222
                     /locus_tag="ACG816_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4920905.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4412..5116
                     /gene="yycF"
                     /locus_tag="ACG816_002046"
     CDS             4412..5116
                     /gene="yycF"
                     /locus_tag="ACG816_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4920906.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5121..6956
                     /gene="walK"
                     /locus_tag="ACG816_002047"
     CDS             5121..6956
                     /gene="walK"
                     /locus_tag="ACG816_002047"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4920907.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6953..8269
                     /gene="yycH"
                     /locus_tag="ACG816_002048"
     CDS             6953..8269
                     /gene="yycH"
                     /locus_tag="ACG816_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4920908.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8270..9139
                     /locus_tag="ACG816_002049"
     CDS             8270..9139
                     /locus_tag="ACG816_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4920909.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9194..10003
                     /locus_tag="ACG816_002050"
     CDS             9194..10003
                     /locus_tag="ACG816_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4920910.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10106..10735)
                     /locus_tag="ACG816_002051"
     CDS             complement(10106..10735)
                     /locus_tag="ACG816_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4920911.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10905..12197
                     /locus_tag="ACG816_002052"
     CDS             10905..12197
                     /locus_tag="ACG816_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4920912.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12273..12935)
                     /locus_tag="ACG816_002053"
     CDS             complement(12273..12935)
                     /locus_tag="ACG816_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4920913.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13160..13444
                     /gene="groES"
                     /locus_tag="ACG816_002054"
     CDS             13160..13444
                     /gene="groES"
                     /locus_tag="ACG816_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4920914.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13495..15120
                     /gene="groL"
                     /locus_tag="ACG816_002055"
     CDS             13495..15120
                     /gene="groL"
                     /locus_tag="ACG816_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4920915.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         5230 a         2571 c         3215 g         4407 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1501 tttgtttgtt atttttttat ttaatctttg tatctttctg gatcaacgaa agtatacttt
     1561 atatagtcat aacgccgatg atcgctacgt gcgtccggca catcagtcac gatatcaaac
     1621 aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta
     1681 ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
     1741 tcccacacct tataaaaatc tttgacaaat ttttctgatg gatcgtattt aaaccaaagc
     1801 agatgttcct ctagctcgat tgtcatgttt ttcacctcaa cgaaagtata cgaactaatg
     1861 tttgttttgt aaaggataaa aatagggtag caaccggtac ccaaactagt acccatttat
     1921 caaaaacgac gaaatttatg taaatagaaa aataagaata aacattgatg tatcaacatt
     1981 tatatatctt tagaacaaat gaaaagggtg gttctcataa gaaaaacatg ttcgcacttc
     2041 taggcaaacc aggatttgaa gaccttgcta aagaattgaa cgaacgcctg taaaataagg
     2101 atttgtcgct agatttaaaa aatcagtgat agaaacatgt ttgctttaaa atgaaactag
     2161 tgatagtttt agtgatagtg aacttaaaaa aagcactcaa tttgagtgct tttttattgt
     2221 tattaaaaat taaaaaacta aaatacatac ccgaatttca tacccgaaat ataaaaaatg
     2281 tcatcacgta catctttttc gaaactaccg aagtagccaa agatgtactg atgacgtggg
     2341 tttggcaccc aatgggccgt gaggggctcg aacccgcgac ccgctgatta agagtcagct
     2401 gctctaccaa ctgagctaac ggcccgtaca aaaagtactt aataatagta gcactaagaa
     2461 ttttaaaatg caagactaca tttaaagaaa atcagaaaaa tctttacttc cttataggaa
     2521 atcattcata gattattcac atttgctcgc tattatataa gcataccaac aaacaaagtt
     2581 cttgcaagag aacaaaccct aacacttttt tcatttttta ctcctttccc gccgtctccc
     2641 cggcgggtct cctgttctta acgataaaag acaagaaggc ttagccttct tgttttttgt
     2701 tttcatggaa tcccgctaat caaatcacct agttaaagca actagattga atgtttatca
     2761 taagtctgat atcatgttta tgaaatgaaa aaaggagcgg ttatgatgga aaaaatgcgt
     2821 cagcaattaa tggagtgggg gaaggaacta gaaacattcc gtctgcctca ctgggaagaa
     2881 ctccctgact tagaacttta tatggaccaa gtcagaacgt tagttgatcg ttatctttca
     2941 cctgtgatac aaggagaaaa gcatccattg cttacctctt ctatggtaaa caattacgtt
     3001 aaactaggtt tgataccagc accagttaaa aaaagatata acaaagaaca cgtagcattt
     3061 ttacttgcaa tcactacgtt gaaacaagta ttgactattc ctgaaatcaa agaagggata
     3121 ttgttccaag gaaaaacagt aggaattcgt gaagcctata atctattttg cgatgaacaa
     3181 gaagcagctg tgtggatggt cagccaatta gcacaaggta aatctcatcc gcaaaaattc
     3241 gaccaggcaa cccctgtaga atatattgct gtaagagcgg caactttatc gtttgcaatg
     3301 aaacttcttg cggaaaaaac gattgttcta gaaaccgaat atttaaaaga ggagaaaaca
     3361 aatgaaaaac aataaaatcg caattttagt agattcaggt acggatgtgc cagaagaatt
     3421 gataaaaaaa tacgagatgt atatgatccc attgaaaatt atttataaag atcaggtata
     3481 tacagataaa attgacatta cctctgaaga gatctatcaa cgtttgccag aggaaatccc
     3541 tggtacatct ttgccggatg gtgagtcgat cacagaaatc tttgagcaaa tcaaaaaaga
     3601 cggttatgaa aaggtacttg cggtaacaat ttccagtgga ttgagcggga catataatgt
     3661 ggttcgttta attgcacaac agcaagaaga attggaagca tatgtactag atacgaaaaa
     3721 tattggtatt ggtgcaggtt tttctgcgat ccaagcagca aaatggctgg aagaaggagt
     3781 agaatgggat caactgatca gtaatctgaa tgagttagtc gaaagaacaa aagttttctt
     3841 taatgttgcg acattggaat atcttcaaaa aggtggacgg atcggtttag ttgcttctat
     3901 tgtaggaacc gctttaaagc ttaacccaat catctcatgt aatgaagaag ggatttatta
     3961 cacagtagga aaagctaggg gacgtaaaaa aagtttggat aaaacggttg cttacgtcaa
     4021 agaaaaaatc ggtaatgcaa aggtcttcaa cttagctgtt gcccatgggg atgcgaaaga
     4081 agaagcaatt gaaatggaag cgagacttaa aagagagttc ccacaagcaa accaaatcta
     4141 ctttggccag atttctccgg cattagtagt ccacactggt ccgggattat tgggtgtcgg
     4201 tgtacaagtt ttagaaggat aaatcaaaaa aaggaaacac tgaggtgaaa atcagtgttt
     4261 tcctttttta ttgaagttag aacaaaaaat aataaagtat tttgcacttt cattttaatt
     4321 ttctcttttg ttttataggc aaagatgaaa caaaagcaga aatacggtat aatgagaagg
     4381 tatgacaata atatataaga ggtggctctt tatgaaaaaa gttttagttg tcgatgatga
     4441 gaagccgatt tcggatatcg tcaaattcaa tttagccaaa gaaggctatg atgtttatac
     4501 tgcatatgat ggggaagaag ccttggaaaa agttgctgag gtagaacctg atttgatttt
     4561 acttgatttg atgctaccta aaatggacgg cttagaagtt gctcgtgagg tacgcaaaac
     4621 ttatgatatg ccgatcatta tggtaacagc caaagattct gaaattgata aagtcctagg
     4681 cttagagtta ggcgccgatg attacgtgac caagccattt tccaaccgag aattagttgc
     4741 acgtgtcaaa gcaaatcttc gtcgcggtgc cacggctgcg aaggaaccag aagaggcagc
     4801 acctgcggaa ttgacgattg gtgatttgac gatccatcca gaagcttaca tggtgacgaa
     4861 acgaggagaa acaatcgagt taacacatcg tgaattcgaa ctactctttt acttggcgaa
     4921 gcaccttgga caagtgatga caagagaaca tttattacaa acggtttggg gttatgatta
     4981 ttttggagat gtacgaacag tggatgtaac tgtacgtcgt ttgcgtgaaa agatcgaaga
     5041 taatccaagc catccaaatt atttagtgac acgtcgtggt gtcggttact atttgcgtaa
     5101 cccagaacag gagtaattgg atgaaaagaa aagtccgctt ttttcgatca gtcaatttta
     5161 agatcgctat tacctttatt ctgattttat tgatctcgat cgaaattatt ggtgcctact
     5221 ttatacgtgg tttagaacgt tccacgatca atacatttat taaagatatg aatcaaacgg
     5281 tggaatcatt agcaacaaca atcagcccgg aattgaatcg aaaagataat gcagatgatg
     5341 aagaggtaaa tgcgaatatc aaaagattta ttgaaaacag tgcgactagc gatatcatcg
     5401 aaatacgggt cgttgacgaa aaagggatca tccgaggaac aaccgatgtc aatgaacaaa
     5461 gcgctgtggg aaagaaaaat gattatgttg atatcaatga tttcacgact aaacgttatg
     5521 ttgctttaga taacgacgat aaacgggtga atatcaatgt tcaaccgatc ctgtccccga
     5581 ccggcgatgc cgttatcggg gctctttacg tcaaaagtaa tatcgaacaa aaatattcgg
     5641 aaatcaataa cacagcagtc attttcttta ctgcctcact aattgcggca tttatttcaa
     5701 tgatcgtatc tgttttagtt gctcgttcga ttacacagcc gatcggtgaa atgagagaac
     5761 aagccattcg aatcgctaga ggagactaca gtcggaaagt caaagtgcat ggacaagatg
     5821 aacttggaca gctggctgat accttcaatc agttagctga acgtattgaa gaagctcaag
     5881 atacaatgga agctgaacgc aatcgtttgg atagcgtgct atcccatatg acagacggcg
     5941 tcattgcgac agatcgcagg ggtaaagtca ttacgatcaa tgatatggct gtttccttgc
     6001 tagatatcaa aaacgaggaa gcaatcggac aatcgatttt gacactttta gatatcgaag
     6061 aagaatatac gttgcggaaa ttattagaag atccaaacga aatggtactt gaccgttcaa
     6121 ccagtacatt ggaaagtgat cagatgatct tgcgagtaga cttttctatg atccgtcgcg
     6181 aatcaggttt tatcagcgga ttagtagctg tcttgcatga tgtgacagaa caagaaaaaa
     6241 cagaacggga acgtcgtgaa ttcgtctcta atgtttccca tgaacttcgt acaccgttga
     6301 caagtatgcg tagttatatc gaagcattga gcgaaggtgc gtggaaagac gaggaagtag
     6361 caccaaattt tttgaaggta acgctagatg aaacagaccg aatgattcga atgatcaatg
     6421 acctcttaaa tctatctcgc atggatacag gtaataccca gcttcaatta gaatatgtca
     6481 atttcaacga gttagtgaat tttgttcttg accggtttga tatgatggtg ggaaatcagg
     6541 aaaaaaatta taagatccgt cgtgaattta cgcaaagaga tctttgggtc gaagtagata
     6601 cagataaaat catccaagta gtcgataata tcatgaataa cgcgatcaag tattcaccag
     6661 atgggggaac gattacttgt cgcttattag aaacgcataa taacgtggtt ttaagtatca
     6721 ccgatcaagg attggggatt cctaaaaaag atctgaaccg agtgttcgag cggttctatc
     6781 gagtagataa agcacgtgca agagcacaag gtgggacagg acttggatta gctatttcta
     6841 gagaagtgat taaggcgcat cgaggcgcta tttgggcaga gagcaaggaa ggaaaagggt
     6901 cgacttttta tatctcgctg ccatatgaac cttatgagga ggaatggtgg gaatgagaat
     6961 ctctgaaaaa gtcgttcgta taggactcat tttgatggtt gcgttaagca tatacttctc
     7021 ttatgcaatc tggctgagcc cagcaggaaa aagttcaatc aacgtagatg agacaaacac
     7081 tcaaatgatc gagtctcaaa gcaatcgaaa aacttccgaa gtctttcttc cgcttcatgt
     7141 tacatggcac cattcgggaa tgatccaaga aaccaacagt gaaaatctcg tgtaccgatt
     7201 gcaattgatc atcgataaag caagatttgg aaagctttct gaagtagttt caggagacaa
     7261 atcacagttt gaacaggtga aaaatctgaa ctctggtttt gagttggcct ataatgcgcc
     7321 ttttttgcta agagaataca aggaagcatt tgatttagat ttagatgtgt cgaacttggc
     7381 aaaagaaagc gaaaatatgt attttactag aatacaattc gatcaaggaa ataaaaaaat
     7441 ccgatttttg aattattcga accatctcgt atatgaagca aatatttcgt tgaattggac
     7501 agatatcaat aaagaattaa cagcaaaaga cacaaaatgg acagaaatga caacagaacc
     7561 atcgacgatc gacacgcaat atgctacgaa acattcgatc aaactgaaaa agtatagtta
     7621 tatcctgtca cagcaaccct atacactttt tagaaatgct tttttccaga aaccagatag
     7681 cgtgaaaaat aatgatgaaa gcaatgaatt gattttttac ggtgggaatg aaacaatgtc
     7741 tatccattcg gaaacgcaag tagttgattt tcgtggagag ctgccagaga aaaaagaaaa
     7801 tgctagtgta ttttctgaaa gtttccccta tattagcaaa ttaggcagtt ctttagggaa
     7861 cctcagatat tttgaccgca gtgacaatac gatcgattat cggatattcg tggaaggctt
     7921 cccggttttt gggacagaca gcagaggaca gattggtgtg gaagttggcg gtgaaacgaa
     7981 tggttctgtg caagtgaata tccaaaccag tttgaatacg attcaagtgc cgataccttc
     8041 agacgaagaa gtagaacttg caagtacgaa tgaaatcatc cagcagttaa aagctaaggg
     8101 agcagatagc aacaagattg gttcagtgat cgttggctat acatggcaaa atgtcaaaga
     8161 aacgaatcgt gtcgttgatc tcttacctga atggtatgtg aaatataaag ataactggta
     8221 ttcagctaac gaccttctgg aaaaattacc tggattggag gcgaattaat tggatttcaa
     8281 aaaaatcgaa tggatatttt tcgtcgcttt tttagggttg aatgtgtttt tattcagcag
     8341 ttatcatgac gcggtatacc aagaaaacaa tgtcattcgc tctaatcaga ctgaatcaat
     8401 ccaacaacgt ctggctagcg acgatatcac ttacacgggg aagatttcgt ctgagaacaa
     8461 gcaaggctat tatcttagcg cggagcagac caatcttagc acttcattag cgaattatcg
     8521 caaggctggt aaaagcggct tgctggcaag tggtgtgttt ttagacggtg atgttttgac
     8581 gaaatcttta tccagttcag atgctgaaaa gaacgtgatc gatccagata aaattagtag
     8641 ttccttggaa gattttttat ctaacaaaga caatatttta ttcggtaatg attatgtgta
     8701 cacgaaaaac ttttctcatt tagaagaaga accactagaa atccaagcaa gccaagagta
     8761 tgaaggcatc ccattcaatg atgatacagc aaaaatcgtt ctttctttag aaaaaaacga
     8821 taatgattat agcatcacga aatacatgca gacgcattta tctgatatcg agcagctgcg
     8881 cgaaaagacg gaactgtata cggaagagga agcaatcaat acattgtaca tcaataacaa
     8941 aataccaaga ggatcaaaaa ttttatggcg tcagctagct tattcctgta ttctgaaagt
     9001 ccgggaaaaa aatgtgtatg ttcctgtttg gcatgtagcg atcgaaacaa gcgataaagt
     9061 ggtccaaatt gagagtgtca acgctttcag caatacgatc gtgacaaaca atactgtacc
     9121 aaaagtggaa gatcaataaa aaagcgagta taatgaaaaa agtttacaaa gtaagaaggg
     9181 aagtaggagt caaatggctg aagaaaaacc agcttttaaa atcagtatcc tagcaagtgg
     9241 aagttcgggt aattcacttt atatcgaaag tgaaaaaaaa cggcttttgg tcgatgcagg
     9301 acttagcggt aaaaaaatca cttccttgat ggctcaaatt gatcggacac cggaccaatt
     9361 agatggaata cttgtgacgc atgaacatcg agatcatata catggcgtgg gtgtcttggc
     9421 tcgtaagtat catttggatg tttttgccaa tcagcagact tgggaagcga tggaaccact
     9481 gattggaaat gtaccagtgg aacaaaaaca tctatttgag atggggaaag tcagaacatt
     9541 cggcgatatc gatattgaga gtttcggcgt ctcccatgac gcagcggctc cgcaatttta
     9601 tcgtttccat aaagaaaatc ggtcattcgt catgttgacg gacaccggat attgcagtga
     9661 ttatatgcga ggtgtcattg aaaatgccga tggttatttg atggaaagta atcatgatct
     9721 tgagatgtta cgcatgggac cttatccgtg gaatttaaaa cagcgtattt taggagatag
     9781 aggacatctt tccaacgaag acggtgcact tgtcatgact gatgtgatcg gtaatcggac
     9841 caaacgaatc tatcttggcc atcttagcaa ggaaaacaat atgaaagaac ttgctcattt
     9901 aacaatggaa aatgttttga aagaaaaaga ttttggcgtt ggacatgatt ttgaaatcta
     9961 tgatacagat ccagatgaag caactgagct attttcgatt taaacagttc aatcaataaa
    10021 aatataaaaa gcagaagata aaatcaatca gggttggttt tatcttctat ttttattttt
    10081 ttagcatatt aaaaaaataa ccgtattatc gactgactac actgatgtgc tgcttttcat
    10141 gtttctcatc cagtatctca tctactaatg caatcgcgta atctgccatg ctgatcgtac
    10201 tgtcgccagc agcgtttctt tgtaaataat catcacttaa caaataagta cctgtacgtt
    10261 tgccatgtgg atcaaataaa gaagctggac tgatataagt ccaatgtaga tttttagatt
    10321 gtttcaattt taacaatcct tcgtacatat tgaaagctgt tggatagaag ggtgcatttt
    10381 taggtacttg gtcaatcata cgagtcgtct tttctgtatc taagaataac gaagaggcac
    10441 ccccaacgac aattaagcgt gtatcggttc cttcaagcat ctcaatcaaa tgggacaaag
    10501 aagtttgatg catttcttct tttcctttgg gagcgttgaa agcatcaacg acaacatcaa
    10561 aagatgcaag atcagtagtt gtcaactcaa ataaatcctt ttgtataaac ggaacatctg
    10621 atggcaattt ttctggatga cgcacgattg ccgtcacgtc taaatctctg gacaatgctt
    10681 cgtctaaaat aaatgaacct gcatggccag tagctccaat gagtgcaatt ttcataaaaa
    10741 acctcctttt ttattcctgt ccattgtaag cagttgctga atattcgtca aagaatccga
    10801 tttttgaaaa gctcagtgcg cattcctttc atggttcgtg atcattgggt atagttaaat
    10861 taagaaaact gattgataaa aatagaagtt gaaggagata agaaatgaaa gagtggttag
    10921 atcgtgcctt tttaccaatg ataacatctg ttgtgaatct tagaccctta actgctttgc
    10981 gtaatggaat gacttacacg atcccaataa ttattgttgg ctcgctgttt ttcttatttg
    11041 ctcattttcc tgtggattac atcgctcagt ggatagacaa aacaggattt tctatgtatt
    11101 ttacacaagt ataccaggct acatttgatg tgatagcctt ttggatcgtt tttgccattg
    11161 catacgctta tgcaaaagaa gaagacatcg atcgcttacc agtcggcata acagccttgt
    11221 ctgcttatct actgcttatt cagccactaa atactcagat agatactgtc tggacaggtg
    11281 ctgaagggat actaggagca gttacaactg ggctcatcat cggatggggc tatgcacgtt
    11341 tagtaagact tgcgaaaggg tggaagcaaa gagagaagat acctgaaaat gtttggcagt
    11401 cttttacttc attgattcca atcattgtta tagtcacttt tacgcttatc ggttcagcgg
    11461 tttttcaaca gttgttcaga atgactgttg ttcaaggagt atggacatat gtccagcttc
    11521 ccattcaatc gttcatagac acgttgtttg gtgtgctttt attaggtttt tttgttccgt
    11581 ttttatggtt atttggtctt catggatctg cgatggtcaa tgggttggtt agtcccgtct
    11641 tgcaagcgaa ttcattagcg aatgcagaaa tcttagcctc aggacaagag ttgacggtgg
    11701 caaatggcgg acatatcgtt actcagcaat ttctcgatca gttcatgacg gttacgggtg
    11761 ctggattgac gttaggggct gtatttttta tgacggtttt tgcgaaatcg aagaaataca
    11821 gagaattagg aaatcttgct cttttaccag cctttttcaa catcaatgaa tcaattattt
    11881 tttccacacc gatcgtgatg aacccgatga tggctgttcc ttttattttc gcaccggtcg
    11941 tttcaggatt gattacctat tccgctttat attttggatt cgttccttta tttacagcag
    12001 tccaagtccc ttggacaaca ccgccgatcc tctcgggttt cttagtaggc ggagtgcctg
    12061 cagcgatttt acaaggtatc gtgttgtcaa ttagtttttt catttatttc ccatttttga
    12121 aaaaaataga ttctgcaaac tgcaaaaagg aaagacagac aaaaaatgat ggagcagcag
    12181 aaaagatgaa cgactaaaaa acaaaaatac tcctgtcgct ttagattggg aaaatcacca
    12241 atctaggatg acaggagtat ttatagtcat gtttatacta gccctttttg gacagctaat
    12301 tgaacaagaa caactaatgt gttcattcct acgtgtgtga tcatcgatgt ccagatccga
    12361 ccagttgctt tgtactctag gctgaagaag aaacctagga aaaagtagat tagcagatga
    12421 ccatcattat gagcaaacgc aaacagcaaa gagctgatca ctgctccgac ccaaaaattt
    12481 gaatatcgac tgatgatccc cagtatactg cggcggaata cgaactcttc catgatgggt
    12541 ccagcaacag ttgtcgccaa aataaaagcg ggtgcttcca tgatcatctg gatgatattt
    12601 tgtgtattct cggaaggggt tgctttgcca aacaatacag attctaacat gacagcgatg
    12661 ctttgcagaa tcaatgcgac aaagattcca accaatccat aaataacaat ctttggagca
    12721 gaagcttttt ttaccgctga ttcgatcgct aagggttcac gttgcttaaa ataaaaaata
    12781 gctaggacaa cggcgcctag tatatacatg actgcagttg ctgtgatcaa ttcagacgta
    12841 gattttacaa gtcctatcga agctaggatc aagggtgaga taaaaattag accatagcaa
    12901 aaaatgctga ggaagctata tttttttaaa gacatgtaat tcctcctttt aagggataac
    12961 aagcatgcaa ggcaagctta tatcgattga tttcaattat atcataattt caataagcag
    13021 ctgtcagaat aaaatttctt ttctttgatt taaaagcttg caaaaaagag ggagaatgag
    13081 tattattaaa aatgtaggtt agcactcgaa ggttaagagt gctaacagat taaaaaaaga
    13141 gttatcggag ggatttatcg tgttaaaacc attaggtgat cgcgtcatta tcgaagtcgc
    13201 aaaagaagaa gaaaaaactg tcggaggaat cgttttagct tctgctgcta aagagaaacc
    13261 ccaaactggg acagttgttg cagtcggtga aggccgtttg ttagaaaacg gtgaaaaagt
    13321 tcctgccgct gtaaaagcag gcgaccaagt tatgttcgaa aaatacgccg gtacagaagt
    13381 aaaatacgaa ggaaaagaat atctgatcgt cgcaggaaaa gatatcatgg cgattgttga
    13441 gtaaatttgg aagataaaaa aataatcaaa aataatcaat gaggtgaatc aatcatggca
    13501 aaagaattga aatttgcaga agacgcacgc gcagctatgt tacgcggggt agataaatta
    13561 gcagatacag tgaaagtaac attaggacct aaaggccgaa atgtcgtact agaaaaatct
    13621 tacggctctc cattgatcac aaatgatggt gtgacgatcg caaaagaaat cgaattagaa
    13681 gatcattttg aaaacatggg tgcaaaactt gtatcagaag tagcatcaaa aacaaatgat
    13741 attgcaggag acggtacaac gacagcaact gttctgacac aagcaatcgt tcgcgaaggt
    13801 ttaaaaaacg taactgcagg tgctaatcct ctaggcattc gtcgcgggat cgaattggca
    13861 acaaaagcag cagtcgaaga attgcataat atctcaactg ttgttgattc aaaagaagcc
    13921 attgcacaag tagcagctgt ttcttcgggt tctgataaag ttggacattt gattgctgat
    13981 gcaatggaaa aagttggaaa cgacggtgtc atcacaatcg aagaatcaaa agggatcgaa
    14041 acagaactag atgttgtaga aggtatgcag tttgatcgtg gttacttatc acaatatatg
    14101 gtaacagata acgacaaaat ggaagctgtt ctagaaaacc catatatctt gatcacagac
    14161 aaaaaaatct ctaacatcca agatattctg ccattgttag aacaaatctt gcaacaatca
    14221 cgtccattgt tgattattgc tgacgatgta gatggtgaag cattgccaac tcttgtattg
    14281 aacaaaatcc gtggaacatt caacgtagta gctgtaaaag ctcctggttt cggcgatcgt
    14341 cgtaaagcaa tgcttgaaga tatcgctatt ttgacaggtg gtacagtaat tactgacgat
    14401 ctaggtttag aattgaaaga tgtaacaatc gaaaacttag gaaatgcttc taaagttgtt
    14461 gtagataaag acaatacaac gatcgtagaa ggttctggag aaaaagaagc aatcgaagct
    14521 cgtgtgcaat tgatcaaaaa ccaaattgct gaaacaactt ctgattttga ccgcgaaaaa
    14581 ttacaagaac gcttagctaa attagcaggt ggtgtagctg ttgttaaagt tggtgcagct
    14641 actgaaacag aattgaaaga attaaaatta cgcattgaag atgcattgaa tgctacacgt
    14701 gccgcagtag aagaaggaat ggtctcaggt ggtggtacag ctctagtgaa cgttatcagc
    14761 aaagtttctg ctgtagaagc agaaggagac gtggcaacag gtatcaagat cgttgttcgt
    14821 gctttagaag aaccaattcg tcaaatcgct gaaaacgctg gttatgaagg atcagtgatt
    14881 gttgataaat tgaagaacgt ggaactaggc actggtttca atgctgctac tggagaatgg
    14941 gtaaacatgg ttgaagcagg tatcgtagac ccaactaaag taacacgttc tgccttgcaa
    15001 aatgccgctt ccgtttctgc cttgttatta acgacagaag cagtcgttgc agataaacca
    15061 gaaccagcag cgccagcagc tcctgcaatg gatccatcaa tgatgggcgg tatgatgtaa
    15121 gaaaaagcaa cttgcttatc tacaactgat cattagtgaa agacagataa aaagaggctg
    15181 gaacagaagc gtttaactcc aagaaataag aagaaattca cggaaattgc ttttcaaatt
    15241 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgctgtt tatacgtgtt
    15301 tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctcttttttg tgtgtaatta
    15361 taatgaaaat agttattttt aatagttata atattttatc tattaaaagt aaatatccat
    15421 tca
//
LOCUS       DAZUKT010000062        15140 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000062 DAZUKT010000000
VERSION     DAZUKT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.063"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACG816_002056"
     CDS             5..2377
                     /locus_tag="ACG816_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4920916.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACG816_002057"
     CDS             2438..3898
                     /locus_tag="ACG816_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4920917.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACG816_002058"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACG816_002058"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4920918.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACG816_002059"
     CDS             6129..6278
                     /locus_tag="ACG816_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920919.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACG816_002060"
     CDS             6353..6946
                     /locus_tag="ACG816_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4920920.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACG816_002061"
     CDS             6959..7858
                     /locus_tag="ACG816_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4920921.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACG816_002062"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACG816_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4920922.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACG816_002063"
     CDS             8724..8984
                     /locus_tag="ACG816_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920923.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACG816_002064"
     CDS             9429..9635
                     /locus_tag="ACG816_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4920924.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACG816_002065"
     CDS             9656..9886
                     /locus_tag="ACG816_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920925.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACG816_002066"
     CDS             9902..10339
                     /locus_tag="ACG816_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920926.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACG816_002067"
     CDS             10332..11039
                     /locus_tag="ACG816_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920927.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACG816_002068"
     CDS             11203..11403
                     /locus_tag="ACG816_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920928.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACG816_002069"
     CDS             11419..11598
                     /locus_tag="ACG816_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920929.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACG816_002070"
     CDS             11923..12186
                     /locus_tag="ACG816_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS4920930.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACG816_002071"
     CDS             12180..12530
                     /locus_tag="ACG816_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4920931.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACG816_002072"
     CDS             complement(12554..13168)
                     /locus_tag="ACG816_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4920932.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACG816_002073"
     CDS             13482..13904
                     /locus_tag="ACG816_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920933.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACG816_002074"
     CDS             13914..14117
                     /locus_tag="ACG816_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920934.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACG816_002075"
     CDS             14328..14912
                     /locus_tag="ACG816_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4920935.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5571 a         2451 c         3062 g         4056 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac
    15001 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    15061 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    15121 tttcaaatta attagtaaag
//
LOCUS       DAZUKT010000063        15048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000063 DAZUKT010000000
VERSION     DAZUKT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.064"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(510..1076)
                     /locus_tag="ACG816_002076"
     CDS             complement(510..1076)
                     /locus_tag="ACG816_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4920936.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="ACG816_002077"
     CDS             complement(1076..2032)
                     /locus_tag="ACG816_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4920937.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="ACG816_002078"
     CDS             complement(2032..2688)
                     /locus_tag="ACG816_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4920938.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="ACG816_002079"
     CDS             3058..5076
                     /locus_tag="ACG816_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4920939.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /gene="leuS"
                     /locus_tag="ACG816_002080"
     CDS             5503..7917
                     /gene="leuS"
                     /locus_tag="ACG816_002080"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4920940.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="ACG816_002081"
     CDS             8265..9818
                     /locus_tag="ACG816_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4920941.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="ACG816_002082"
     CDS             complement(10199..11983)
                     /locus_tag="ACG816_002082"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920942.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="ACG816_002083"
     CDS             complement(11998..13719)
                     /locus_tag="ACG816_002083"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4920943.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="ACG816_002084"
     CDS             13819..14580
                     /locus_tag="ACG816_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4920944.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4899 a         2761 c         2971 g         4417 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtagcc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaactcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    14881 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    14941 acattattac aatatgttaa taaggcaaaa ataaatagat ggattgaata aacatatttt
    15001 ttaatcaaat agtccctact gtgactttcc tcagctatcg gtgatact
//
LOCUS       DAZUKT010000064        14993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000064 DAZUKT010000000
VERSION     DAZUKT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.065"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..551
                     /locus_tag="ACG816_002085"
     CDS             42..551
                     /locus_tag="ACG816_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4920945.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            644..1806
                     /locus_tag="ACG816_002086"
     CDS             join(644..920,920..1806)
                     /locus_tag="ACG816_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4920946.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2086..3066
                     /locus_tag="ACG816_002087"
     CDS             2086..3066
                     /locus_tag="ACG816_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4920947.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3188..4606
                     /locus_tag="ACG816_002088"
     CDS             3188..4606
                     /locus_tag="ACG816_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4920948.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4677..6146
                     /locus_tag="ACG816_002089"
     CDS             4677..6146
                     /locus_tag="ACG816_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4920949.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6156..6623
                     /locus_tag="ACG816_002090"
     CDS             6156..6623
                     /locus_tag="ACG816_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4920950.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6659..7531
                     /gene="scrK"
                     /locus_tag="ACG816_002091"
     CDS             6659..7531
                     /gene="scrK"
                     /locus_tag="ACG816_002091"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4920951.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7612..10146
                     /locus_tag="ACG816_002092"
     CDS             7612..10146
                     /locus_tag="ACG816_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4920952.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10302..11075
                     /locus_tag="ACG816_002093"
     CDS             10302..11075
                     /locus_tag="ACG816_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4920953.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11068..11859
                     /locus_tag="ACG816_002094"
     CDS             11068..11859
                     /locus_tag="ACG816_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4920954.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11869..12360
                     /locus_tag="ACG816_002095"
     CDS             11869..12360
                     /locus_tag="ACG816_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4920955.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12378..12788
                     /locus_tag="ACG816_002096"
     CDS             12378..12788
                     /locus_tag="ACG816_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4920956.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12792..14192
                     /locus_tag="ACG816_002097"
     CDS             12792..14192
                     /locus_tag="ACG816_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFS4920957.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14215..14589
                     /locus_tag="ACG816_002098"
     CDS             14215..14589
                     /locus_tag="ACG816_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4920958.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5212 a         2104 c         3013 g         4664 t
ORIGIN      
        1 aaacaaatta tttgtgtttt attgtttagg gctatactat attggtacaa ggaatagaaa
       61 gaggtgttta tgtggttgat aaaattcagt ttaatcaagt tatagaagaa ttcatcaaag
      121 taaataattt aatgacccat attcaaaaac gaccaatcag aataactaat gatctaaaac
      181 tcagtacgag tatgattcat ttgattgatg tgattggaaa ttatcctgaa actagtatta
      241 cggaattagc tgatcgatta ggcgttacca aaggagctgt atcccaacag attcccactt
      301 tagagaaaat gaatcttata agcatttctc aaaaaaaagg aaataagaaa actaaacttg
      361 tatcgttaac aaaatatggt cgaaaggtgt acgaatcaca taattattta catgaagagc
      421 tttatagctc aataaatgaa gcgttagaat cattctcacc agaccaaatt tcaaccatta
      481 aacagattct agaaaaagta tcttatacaa tcaatgaata tcaagtaagt ctgacaagaa
      541 aggaagattg acatgaaaaa aatgattttt gaatcggcta cactagacta gacagaaaaa
      601 ataaggtgtg taaaataaat gaaaacacac tggaggaatt tttatgtcaa gacgtcaacg
      661 tagaacctat tcaaaagaat tcaaacaaca agtcgtcgat ctttatctcg caggaaagcc
      721 ccgagctgaa atcattcgag attatgagct gactacctcg gcctttgata agtgggtcaa
      781 gcaagcgcaa actactggct cgtttcagga aaaagataac ttgatccctg aacaagtaga
      841 actaattgca ttacgaaaga aaaataaaca gcttgaaatg gagaatgaca ttttaaagca
      901 agcggcgctg atattgggac gaaaagacaa gtaattgata ccaataagca caaatactca
      961 atatcagcga tgtgtaaagt gttaaacatc tcttgccaaa cctattatta tcaagccaaa
     1021 ccagttgaag atgaatcaga actggaagaa ctcgttcaag aagaatttat tcgcaatcga
     1081 aaagcttatg gtaccaggaa gctgaagcag tgcttggcca atctaggtct tcaagtgagt
     1141 caacgcagaa tcggccgtat gatgaaacag cgtggcttgc gttcaacata cacattggct
     1201 catttcaaat tccattcttc tacttgtaac gaagccaaga ccgccaatat tctagacgga
     1261 accttcctac aggaaaagtc attagaagcg attgtaaccg accttaccta tgttcgtgtt
     1321 gggaagaaat ggcagtatat ctgcctgatt cttgatttgt ttaatcgaga aatcatcggc
     1381 tattcatgcg gggagaagaa agatgcgacg ttagtcaagg aagcttttgc aaagattccg
     1441 tactcgttga ataatgtaca actttttcac acggatcgtg gaaaagaatt cgataaccag
     1501 acgattgacg aaattttatg ggcctttaat atcacgcgtt ccttaagcaa aaaaggctgc
     1561 ccctatgata acgccgttgc agaatccacc tataaatctg tcaaagtaga attcgttcat
     1621 caataccgct ttgagacatt ggctaatttg cgattagagc tatttgatta cgtccattgg
     1681 tggaatacca tccggctgca cggcacgtta ggctatgaga caccgatcag ttttcgacag
     1741 cagagattgg cgcagcgaat ccttgataat gagctcggat gtgataccgg tggcgaggca
     1801 ccttaattat ttgaatgagc ttctgcctta gagaatcaga ttcgaggagg ctcgttgtca
     1861 agggcagtcg gaacaccagc agcaagctta cgcaatacct tataattttt gtcaaaaaaa
     1921 gtgttgccat tccacatata tctttaaaca gctattgcca gataaaaata aatgatatta
     1981 tatatataaa tacttatccg aaaaggggaa tgagagagtc aaggttcttg gaatgtcgaa
     2041 tagtcgagta ggtagatttt ctatctacaa ccttgttgtt taaaaatgaa aaaaattgga
     2101 attagagaag ttgcgaaaga ggcaaatgtt tctcctacga ctgtgtcgag ggtattgaat
     2161 gatcgtggat atttgagtga ggaaacaaaa agaaaagttt ttgatgccat gaaaaaatta
     2221 gaatactatc ctaatgaact ggcgagagca ctttttaaaa acgaaacata taccgtaggt
     2281 ttgatttttc ctacaattac taatccattt cacgctgagt taattcaaga tatcgagttc
     2341 gagctttcca gcaggggata taaagtgttg ctgtgcaata gcttaaacaa tccaacaaaa
     2401 gaaaaagagt acttgacgat gttgagaaag aatcaggttg atggaattat agtaggaacg
     2461 cacaataaag atattgcgga ttatgatatt ccagggctac ctattgtagc gattgataga
     2521 agtcttggtg aaaatacagt tacagtatct tgtgataatt acaggggtgg ggaattagct
     2581 actcaactac ttgtaaatca tggctgtcag aatatactgt gtattcgggg agatagtaag
     2641 ataaagctac ccgcgaatag taggacttta gcatataaag atgttttgaa taaattcatg
     2701 ctggaaccgt tagtcctaga agtaccattt gtaaaagaaa tcaatgaaaa aaatgaaata
     2761 attaagacat atctggaaac tcatccaaat attgacgggg tttttgcagg agatgattta
     2821 ttggcttctt tggcgattaa caacttagga agttcgggca aaagagttcc aaaagaggtt
     2881 aaagtgattg gatttgatgg agctcaacaa acattgattt ataatccaga attatcaact
     2941 attcaacagc ctatagatca aatatctaaa gtagcagtaa aaaaattatt gaatatgatt
     3001 aagggcgaaa aggagacgga tgaactttca cttccagtaa agctcataaa aaaaaataca
     3061 gcataagtta aatcggcgaa aaattccttc gtcgatttaa aaatattttt atgaaacggg
     3121 ttgacatatg aaacgggttg acatatatta gttgtgaaga taaaaacctt ttcactggag
     3181 gaagaaaatg gagaaattat taattaaaag caataatcat caactttact accaacctaa
     3241 ggatgtttgg gtcggagatg ttatgcctta cgggaaagaa ggaaaattct ttatttatca
     3301 tcaacgtgat actagaaatc cagttccttt tggagaaccg tttggctggg ctttagcaac
     3361 tacgaaagat tttgttcatt ttgaagactt tggggaatct cttttaagag gggatgacaa
     3421 tgagagagac cagtttatct acgctggttc tgtatttgag gcaaatggaa aggttcacgc
     3481 attttataca gggtataatc gtgaattctt agcgcaggga aagacatctc aagtgttgct
     3541 tcatgctgag agtgatgatt ttgttcattg ggaaaaatca gaaacagcat tggagttaga
     3601 accacaagaa gggtatgata ttcgaaactg gagagatcca tttgtcgttt gggatgatga
     3661 aaaagaagaa tatttgctaa ttttaggggc gcgaaagggt caggataaac atctacaaac
     3721 cggacgttta gtggaattta catcaaaaga tttaactaat tgggaattca aaggtgattt
     3781 ttgggctccg ggattgtata ccatgtttga aatgccagaa cttttcaaga ttggtgactg
     3841 gtggtatctt gtctattcag aatactcaga tggaaataaa atactctatc gaatgagtca
     3901 aagtttggaa ggtccttgga ttaagcctaa agatgaagct tttgatggac gggcttatta
     3961 tgcaggtaga acagcttatg atggaaaacg tcgagtccta tttggatggg taccaacaaa
     4021 agatcaagaa aatgataaaa acaattattt atggggaggt actttggttc ctcatgaagt
     4081 ctatcaaaaa gaggattttt cacttggggt aaaacccata gacgaagtgt ggaatgcatt
     4141 cataaattta catgatattg ccgatgtaga gatagaaaca gcagacacaa gagaagaagt
     4201 aacgcttgtc aaaaatagtg gaactttgtt taatttagaa acaacaattt catttgatga
     4261 gaatcttagt gacttttcac tgagattata taaaaacctt gagacagatg aatcttacga
     4321 atatcgcttc aatttagatg aggggcgtct tacatttgat aaaaaccctt gttacccttg
     4381 gtttcaatat atggataaag gacttattag acctatatct ttaaagccta accaatctta
     4441 taaactaaaa gtgattgttg atgacaatat aatgacactt tatattgatg gaatcgctct
     4501 taacgctcgt gtacatggtt gttttggtca agcaattact attacagcaa caaatggatc
     4561 aatgactgca ataaatacca agttttcaaa tgaaataaag aaataataaa cgtcaatcat
     4621 gtggcaggtc tgaaaatcaa acatgacctg tcacatttaa aagggaggat aaatgaatgg
     4681 actacaaaaa aattgccgaa gatgtattgg aaaacgttgg aggaaaagag aatatctcaa
     4741 gcgctacaca ctgcattact cgtttacgtt taattcttaa agacaaaagc aaagcgaata
     4801 tggaaatgct tgaggaaata gaaggtgcaa aaggtgttct tttcaatagt ggacagttac
     4861 aagtaatttt cggaacaggt ataattgaaa aggtttacaa agagttcgtt gaattgacag
     4921 gagctaagga agtatccgtt tctgaaatga aagacgaagg agtcaataat tcaggaaata
     4981 gacttcaacg agcttttaaa gttttctcgg atatctttat tccgatcatt ccagctttta
     5041 ttggggcagc aatggtttta ggaattcgtt cgttgattac cactcctgga atgtttggaa
     5101 tggatggctc attcgctgat cacaatgtat tcctaaatga tttatccaaa tttctgggta
     5161 ttattgcaac cacatttgat tatttaccag ttttagttat gtattctgca acaaaacgtt
     5221 ttggcggaaa ccctatttta ggattacttg taggatttgt tatgattcat ccaggactag
     5281 ccaatcggaa tgatttcgtc atgggaacta taaagcctga ttattggcat ttatttggat
     5341 taagcattcc agcagttgcg tttcaagggg gtgtttttcc agcaattttg acttcttggt
     5401 ttttagcaaa agttgagaaa attacaaata aatattctcc tcaagtactt gcttacattt
     5461 tagtcccaac tgtgacaatt gttcttgcca actttgcact atttttagtg tttggtccat
     5521 tggggaattt tatcggtaat gttttaggtg gaatcattga tttcctatat atgaagatgg
     5581 gtgcctttgg tgcttttgtt tttgcaggcg cgttacaacc attagttgta acaggaacac
     5641 aacatgctat tcaaggtatt gaggcaaact tagtcgcaac aactggattc aattatatcc
     5701 aacctatttg gtcagtttca attattgccc aaggtggcgg ttgtattggt atgtatttac
     5761 tctttaagaa aaaatctaaa gatagagata ttgcgatttc aagctttatt ccaacactgg
     5821 taggtattac tgaaccggct atctttgctg ttaatttgaa atattcaatc gttccttttg
     5881 tttgctcgtt tattggtgct ggttttggtg gagcatttat gaaaattttt gatgtaaaag
     5941 ccattggaca aggattgact gtattgccag gtctgactat tgctaatcca gttcttcctt
     6001 atatatttgg taatctcata gcattcattt taccaatcat ttttattctc gcttatgata
     6061 aatataaagt gtttatccct gaagctaaac accgtaaatt tgcagaaaaa actgactcaa
     6121 aaaaattgcc agttcaagta aagtgagatg agagcatgtt aaattttttt agaaaaaatg
     6181 aaataagcgt tgtagcacct gcaaccggtg tttttgaaaa actggagaaa ttaagtgatc
     6241 cagtcttttc aaaaggaatg atgggacaag gatttgcaat tgatgcaaaa aacgaaacga
     6301 ttgtatctcc aatcgatggt tttgtttcaa gtatttttcc tacaaaacat gccttaggaa
     6361 taaaaacaaa atcaggaatt gagatacttg ttcatgtagg aatagataca gttgagctaa
     6421 atggtgaggg atttgatatt aaagttcaag aaggacaaaa agttaaggct ggggatattc
     6481 ttatgaccat taattttgta gtttttgaaa agaatcacaa gactaaagat gtcattgttg
     6541 tttttccaga ctaccaagac gaaattttca ttccgaaact tgaagaagta aaacaaggac
     6601 aagaattgtt tgttcttggc taatgataag taattaataa tagaatcgag gaaaatgtat
     6661 ggtttttggg gctattgaag ctggtggaac aaaatttgtt tgtgccattg gagataaaga
     6721 tttgaacata tttgaaaaag tcagctttcc cacgactaca cccaaagaaa caatgaattt
     6781 agttttagat ttttttaatc atcataaaga tgatttagaa ggtatcggag tgggatcgtt
     6841 cggtcctatt gatattcatt gtgaatctaa aacgtatggc ttcattaaat caacgccaaa
     6901 acttgcttgg caggattttg attttattgg aagtttaaaa aagcaactta atattccaac
     6961 tgcttggacg actgatgtaa atgcctcttg ctatggtgaa tatgtttctg gaaatggcag
     7021 aggcctttca agtgttgttt actataccgt aggaacgggc ataggaggtg gcgctatcca
     7081 aaacggaaac ttcattgaag gttttagtca tccagaaatg ggacatatgc ttgtaaaacg
     7141 acacaataaa gacaactttt ctgggacatg tccattccac ggagactgtt tagagggctt
     7201 agctgctggt ccggcaatcg aaagaagatc agggaaaaag ggacaagaag tgactgaaaa
     7261 tgacgttact tgggaaatag aagcttatta cattgctcag tgtgcatata atacaacatt
     7321 gatgttatca cctgatgtca ttatatttgg tggcggagta atgaaacaaa aacacttaaa
     7381 aaagaaagta caagataagt ttattgagtt gatcaacgga tatgtcgcta ttcccgatgt
     7441 tgacaattat attatgacac caaaattgtt agataattct ggtattgtag gttgtttagc
     7501 tttagcaaga gacgttaaag aaaaatctta atttggaaaa aagatcgtct cgactatttg
     7561 tttggttgat gcgatctttt tttcaattag taattgaagg gacgcctaaa agtgaaaaaa
     7621 aaaatgaaag atgaagtatt agagtatatc aaaaataatt taaagtatta cgatttttct
     7681 gctcaagata ttgcaatgaa attttgtatt aaaagaaatg tcgcttccca ctatttgaat
     7741 cagttatttt cagatggaaa attactaaag aatgattctg ttagaccagt aatgttcaag
     7801 tataatcaac aaaagccaaa agactgcttt tctaagttta ttggagcaga catatctttg
     7861 aaatccacta tagataaatg caaagctact gtaatgtatc cccctaatgg cctcccatta
     7921 ataataaaag gaaacagtgg cgttggaaaa agttttttag caagtttgat ctatcagtat
     7981 gctcttgata gaaaagtaat tcataatgac gcaaaatttg ttgttgtgaa ttgtgcagat
     8041 tatgcaaata atccagagct attgtcagca gttttatttg gatataaaaa aggagccttt
     8101 actggggctg aaaaagatac tgcagggatg ctatcttctg ctaatggagg ataccttttt
     8161 ttagatgagg ttcacaatct aagtgcagaa aaccaagaga aattgtttct tcttatggat
     8221 tctcaaaagt atcgtaaact tggtgaaagt gtgcaatggg agtatgcaaa tgttcgcctt
     8281 atcttagcca caacagaaga taaaaatacc tctttgttag caacttttag aagacgcatc
     8341 ccagctgaaa tcactctgcc agattacaat aatagaagta catctgaaaa aattcaatta
     8401 ttatttgaat ttcttaaaga tgaagcaatt acgattgatt caaaaatatt ttgttcgatt
     8461 gaacttttta ctgatctttt atcgaaaaca tttgaaggaa atgttggaga attgaggaat
     8521 gaaatcaagc ttctttgtgc agaaggttat ttaggaaata agagaaagtc ttcaatttat
     8581 ttaggggaca aactagacag cggtttttgg atagatccag aaattagtat agatttaaaa
     8641 aaaatgtttc ttaaatcact ttctgaaatt gatttgattg aactttttca aaatcacatt
     8701 tcaattgaag aaagcatgag tcaattaaga gatagaatac ttaaagagtg tgcaggttct
     8761 tctgagtata attatttaga aagtgacgaa tttatagcat taagaaacta tgttgttaat
     8821 aaaatatcac caattattga ttcgacaggt ctatgtttat tagatgaatt cttggcggat
     8881 atcagtctat ttataatgtt tattgatgta attgaagaaa catcaagaaa ttttttgctg
     8941 tctaagttca gaaaatttcg tatgaaaaat gataaacaaa aattattggc aaatgagatt
     9001 tggagtagtt tagaagtcga agaaagtaaa caagaacttt tgttgaaatg gatacttttt
     9061 cttgtaaaga agttctatgt gaaaatacca gaaacccatt gtctgatagt tatgcacggt
     9121 aaaataacag cttctgcgat cgcaagggaa acaaataagt tgctgaatac atatgtttat
     9181 gaggctttcg atatgccaat tgaaggtgag actagtgact taattaacct aatcaacaat
     9241 ttttgtaaaa gtatagatac gacgaatgga ctcattctgc tagttgatat gggatcattg
     9301 gaacaaatgt atgaaaaaat tgaatccaat gtaataggtg atttggtaat tttaaacaat
     9361 gtttctacag ctttagcgat tgaatgtggg attcaagttt gccaaaaaaa gccaattagt
     9421 catttttacc agatggattt tgattcgttt caagttaaag ttcagtatta taaaggatta
     9481 tcacaaaaga aaaatgtaat agtatcttgt ctctctggtg aggggatttc tgaaaaagtt
     9541 aaggatattt taaaacggta tctcgataat caagtggaga ttttgatttt tgattttaac
     9601 gcattaaaga aaatagcaaa ggaaaaagat actatgattt ttaagaatac aatttgtgtt
     9661 ctttcaacca ctgaatttta cattcaagga atcgattgtt taaatttaga aaatctaatt
     9721 aatgggaatc aaaccttaga aaaattaaat aagtatatgg actcggatag ctgtgagttc
     9781 tgcttaaatg aattagttaa actctttact attgaaggcg ctagtactaa gcttcgcttt
     9841 ttagattcca aaaaagtatt caatgagata gaaaaagtac tatatttgta tgaaaaatat
     9901 tacgatgtca aaatcccaag ttttctgaga attaatctat ttctacatct ttctggaatg
     9961 attgaacgaa ttatgattgg tgatggaata acaaatgaac atttgaaaga gggagcgcaa
    10021 ctatttgata cttttttaaa tgtatcaaaa gatttttttt cagaaatcaa agatatttac
    10081 tgtattgaaa tacccaaagg agagtatgag ttaatctata caatttttga gcagactatt
    10141 ttctaaatag catactagcg tctaaaaaaa tgaaagggtg tccaaagtat atttagacgc
    10201 tcatttaatt aatatttact gtattttccc gctttattgg ataatgttag ttggcacagt
    10261 aattgcttat ataaaagtat aagaagaaag gagagaacaa aatggcgttt tggcaaacat
    10321 ttttagttgt gttgtatgga tttttcatta actgggataa ggaagggccc aaaatcggaa
    10381 cctcccaacc tgtcgttgca ggtttcttga ccggacttat tatgggagat attcaaactg
    10441 gactttatat cggtgggact ttgcaattaa tgacactagg tattactagt tttggtggtg
    10501 catcagtacc ggattatcaa acagcagcat tgatcggtac ttatttagca atctcaacta
    10561 accaggatgc ttctatagga ataactttag caataccggt agcaatgtta attgtacaac
    10621 tagatgttct aaagtggtca acaaacattt tttttcaaca aaaggcagaa aaattcgcac
    10681 aaaaagaaga ttatcaaaaa ttagagtgga tgcaatattt ggcatcaatt aatacaagct
    10741 ttgtatctgg aattcctgtt ttgttaactg tgatattagg cccaacgtta gtgggaagtg
    10801 caatcaatta tattcctgaa tgggttaccg gtggtttaaa ggtagcaggg ggactattac
    10861 ctgctgttgg gataggaatg ctacttaaat atttacctac aaaagattac tttgcctatt
    10921 tgttagttgg tttcgttttg gcagtttatt tccaaatacc aattttaggg atttcgttac
    10981 ttggcgctgc aatcgcacta atcgtatata aaaatagatc aaataatact caagtagcac
    11041 cagctgcaac aggaggtttt gacgaagatg aataagaaac tgacaaaaat cgacaaacaa
    11101 gtaattttcc ggtggattct caatggtgca acttcattaa attatgaaaa aatggaaggg
    11161 ttgggttatg cgtattctat gcttcctttt atcaaggaca catataaaga tgatcctgaa
    11221 ggaatgaaac aatccgtttt agctcactta caatttttta acactacgcc gtatacagcg
    11281 ccttacatta ttggtatgaa tgttggaatt gaaaaagaag aaaaaaataa gtctttggaa
    11341 gcagtaagtg ctctgaaaac aggtctaatg ggtcctttag cgggaattgg agatagtgta
    11401 tttgtagtta ttccatggac tatttttgga gcaattgcag ctaatatggc attggaaggt
    11461 agttttgttg gaattatttt gtggatactg gcatcaattt tattaaggat tactgcttat
    11521 cctttatacc ttgctgggaa agaatcgggt gcgagcctac taactcaaat tgaaacaaaa
    11581 ttaaagtcta ttactgatgc agtatcaatt ttagggttaa tggtggtcgg tgcactcatt
    11641 gccacagttg taaaagtgaa tgttggcttt gaatttaaac aaggcgactt aacaatgacc
    11701 ggaaatgaaa tctttgatca agttatgcct ggtttattac ctgcatgtgt agtagcaatt
    11761 gtgtattatt tgttaggacg aaaggttaaa ccagtttatg taattctatt aatcttagta
    11821 gtttctattg cgttaagagc gttaaatatt ttgaagtagg tgttgaatat ggatgaaaaa
    11881 atgaaaggta ttatacatgt tcgtattgat gatcgaatga ttcatggaca agtagcaaca
    11941 caatggacaa atcaattgag tgctaacaga attatggtta ttaataatga agtagccaat
    12001 gatgacgtaa aaaaagctgt agttcgcctc gctgcaccac ctaatgttag aacttcaata
    12061 attacaaggg aagtagcagt taaaaatatt ctttctggta aatatgaagg acaaaaagtt
    12121 cttattgttg cagtttctcc aatcgatgtt aaatttttga ttgataacgg tttaccaata
    12181 acaagtgtaa acgtaggaaa tttatcaaga aggaatggaa cagaacgcat ccgtccgacg
    12241 ataaatatta ctgaagaaga gagagaggct tttaaagaac taattagtga tggagttgaa
    12301 gtgacagtga tacaaactcc tagagattca aaagaatttt taaaggattt tatttattaa
    12361 gaacagagga tgagtggatg gagtttataa ttacatctca tggaaatatt gccattggat
    12421 ttgtagatac attaaaaatg ttttttggcg ataatttaaa ttgtcatgtg gtaacacttg
    12481 gagatgcagg cattgaagaa ttccgtgaaa acattgaaaa aatagtttct caagtaaaaa
    12541 atcaagaagt tatggtattt gctgacttga ttggtggaac acctttcaac gaatttgcta
    12601 aaagaagtga agtatttcaa aatgggtttc aacttcttgg tggcatgaat ttaggaattt
    12661 tgattgaggc tgtcaatttg agattacaag gaaaaaaaag cgatgatatc atcgaaaatc
    12721 taagaaatat gaatacaatt gcgtgtcttg cagatatgca aagacatact aacgaagagg
    12781 atgagtgata aatgcaaaaa attttttaca agcctaaaga tttttggttt ggggattgca
    12841 tgccttttta taaagatgga actttttatc tatttcatca acgagatacg agaaatccta
    12901 aacctttaac tgatcctttt gggtggtcac tagttacaac cactgatttt gtgaaatttg
    12961 aagaatacgg ggaagtaatt catcgtggtg gcaacgatga cattgatcaa tacatttacg
    13021 ctggaagtat tgtccaagga atcaatgagt atttagcttt gtatactgga cataatcgca
    13081 aagctaagtt ggatggaaaa acttcagaag tgttaatgat tgcaagttct aaagatttaa
    13141 ttcattggaa aaaggatggc gaagcagcaa aacttgttcc tcaagaagga tatgataaga
    13201 atgattggcg cgatcctttt gcaatttttg atcaagatca tggaaaatac gttttgatac
    13261 taggagctag aaaaccagca agtaagaaaa agccgacggg gagattggtc tattttgaat
    13321 ccgaagattt aaaagattgg aatttcaaag gagactactg ggctaataat gaatttaaca
    13381 tgattgaaat gcctgatata tttgaaattg atggaacgtg gtatttactt ttttcagagt
    13441 attgtgaaga taaaaagact aaatatcgag tcggagcagg attattttca gattggaaat
    13501 caccagtaga cgaagctttc gacggaaaag cctattatgc agcaagaaca gtcggaacta
    13561 atgaaactgg ccgttttcta actggctggg tggcaacaaa agaaggggag aacgatcaaa
    13621 ataactggga ttggggcggt gcacttgttc cacatcaaat tattcaaaag acagacaaaa
    13681 ctcttggtgt taaactgcct gaaaaaataa ttgagaattt taaagaaatc actccatttt
    13741 caagttttaa gttagagaaa gaatttggaa aaaaagaaag agtaattgtt cctaaaatcg
    13801 aagattattt tctatttgaa acaactttga ctattacatc gttaacaaag gagtttgctt
    13861 taaaattatt tgtagatagt gatactcaag aaggatacga atttcgtatt tcattgatta
    13921 ataatcagct tactttcgga aaaactccga actatccttg gcctcaaatg tttgataagg
    13981 ggctgcaacg accactattt ttggaaatag gcaaagaata tgaactaaag ttaattgttg
    14041 atgatacgat agctgtttta tatattgata atgttgcttt aagtactaga atgtatgata
    14101 aagtcggaac agcattaagt atgactgtta cggagggagc aatcaaggta gataaactag
    14161 actattcaat aaattatgaa tgtggatttt aaaacaattg aagggatgat gtaagtgaaa
    14221 ttaaatggtt tacaacatat tggaattcca acaaaagatt ttggagcttc aaaaatcttt
    14281 tatgaatcct tagggtttag tctgattaat gaagaaatga atgtgggaag taaagtcggc
    14341 tttttcgagt tgaatggaac gataatagaa atatgggaag ataaaactac tgagtgtaga
    14401 ggagccattg atcatctagc attagatacg gatgacatag atgaggtatt caattcaatt
    14461 caagatttag gatatgaatt attagataaa aatattatgg aattaccgtt ctgggaaaat
    14521 ggaatccgtt actttaattt ctacggacca aatcatgaga ttatagagat aagccaaatg
    14581 aagaaataaa tagataacga agtgctctaa gttggtatga acgaaaagta cttcattttt
    14641 tggtatcagc atttcaatag tttaatgaga atttactatt tcaaattaat tcaaagtgac
    14701 agtatttttt acttatttgg tttcaaattt tgtaatcctt cctatttatt ggaatacact
    14761 ccacattttg tatacaagtc atatcatgtt ttagcaaagc tcttaccaag caaagttata
    14821 atgatgtttt ccaaagtttg atctttcgat ggaggctaac acatattttt tgcaggacag
    14881 ccaaaattag atgtatagaa tagagtctgt gaggcgtata gacagtttat tgtctgtatg
    14941 ccttttttta ttttgaaaaa tttttagtat ttttcgaaaa atgctcactc cta
//
LOCUS       DAZUKT010000065        14610 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000065 DAZUKT010000000
VERSION     DAZUKT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.066"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(216..542)
                     /locus_tag="ACG816_002099"
     CDS             complement(216..542)
                     /locus_tag="ACG816_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4920959.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(554..754)
                     /locus_tag="ACG816_002100"
     CDS             complement(554..754)
                     /locus_tag="ACG816_002100"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920960.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            877..1479
                     /locus_tag="ACG816_002101"
     CDS             877..1479
                     /locus_tag="ACG816_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4920961.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1534..2700
                     /locus_tag="ACG816_002102"
     CDS             1534..2700
                     /locus_tag="ACG816_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4920962.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            3117..6344
                     /gene="fss3"
                     /locus_tag="ACG816_002103"
     CDS             3117..6344
                     /gene="fss3"
                     /locus_tag="ACG816_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4920963.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            6456..6770
                     /locus_tag="ACG816_002104"
     CDS             6456..6770
                     /locus_tag="ACG816_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4920964.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            6783..7157
                     /locus_tag="ACG816_002105"
     CDS             6783..7157
                     /locus_tag="ACG816_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4920965.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            7167..7511
                     /locus_tag="ACG816_002106"
     CDS             7167..7511
                     /locus_tag="ACG816_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4920966.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            7580..8596
                     /locus_tag="ACG816_002107"
     CDS             7580..8596
                     /locus_tag="ACG816_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4920967.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            9190..11064
                     /gene="ltrA"
                     /locus_tag="ACG816_002108"
     CDS             9190..11064
                     /gene="ltrA"
                     /locus_tag="ACG816_002108"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4920968.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            11166..11546
                     /locus_tag="ACG816_002109"
                     /pseudo
     CDS             11166..11546
                     /locus_tag="ACG816_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            11591..12733
                     /gene="dcm"
                     /locus_tag="ACG816_002110"
     CDS             11591..12733
                     /gene="dcm"
                     /locus_tag="ACG816_002110"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFS4920969.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            12723..13012
                     /locus_tag="ACG816_002111"
                     /pseudo
     CDS             12723..13012
                     /locus_tag="ACG816_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13268..14452
                     /gene="mobT"
                     /locus_tag="ACG816_002112"
     CDS             13268..14452
                     /gene="mobT"
                     /locus_tag="ACG816_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4920970.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            14449..14562
                     /locus_tag="ACG816_002113"
     CDS             14449..14562
                     /locus_tag="ACG816_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4920971.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         4947 a         2487 c         3012 g         4164 t
ORIGIN      
        1 atatatatca gaattagaat agccttgctt ttcaactaag ctttcaactt ctttttcatt
       61 cattgtcaca cctgcttctg attttatttg ctataataga ttcaaataaa gttttctcag
      121 aactttatta ttgagtgttc ccccgatctg caaactgacg ggaacactct ttttaatttt
      181 ttctacttca tcaaatttaa aaaacgaggt gttttttaaa tttgatgact caatatccat
      241 tttttgcatt cttcttttga aaacaactcc attttttcta ataaaataac tggcaatccc
      301 attcttttaa atttcaaaaa agttgtatat gatacattca aatatttagc catctctttt
      361 ccttttagaa atggtctttc taatccggta tcaattcttg cttgatcaat agcctctttt
      421 gtcatttcaa aaacataatt tttcaattga ttcgtttgtt gtttatctaa cattactgta
      481 attcctgcat ccattatttt tctcctctca ttttctattt cttcaatcag ccaactcgcc
      541 aagttgtatt tatttagatt ttcaaatcat taagtaacca atttaccaac ttttgataag
      601 tagatgctct aatactttct ttgctcccat tttcgatttt gacatatgtt ttccgattta
      661 tacctatcaa ttttgctgca tcgtttttag taagtataag atcggctcta tgtcttctta
      721 agcttctaat gaattgatca ttaagagtta tcattcagcg cctcctttca aattattcac
      781 tttgtggttt ttatcttaat aagcacattt taaaccacaa aacgagtaat atcaattaaa
      841 atattactca atttgtggtt ttcatgctat aatagtttgg aatttactat agggggcgtt
      901 actttgaata gaataaaatt ttatattgat tccagtaaaa agaccattaa acaaattagt
      961 acagaaactg gtattaacta tacaactctt acgaactatt accgtggtga gcgaaatcct
     1021 agaaatactg aaacatggaa tatccttgct gattatttcc atgttcctgt aagttatttg
     1081 atgggattag acgaaacaaa ttccatagaa caacctaatc ttgatagaat aaaaactgct
     1141 cgtttagaaa aaaaggacaa aatagtcaag aatattgcca gtcttgactc agattatttt
     1201 actgaattat ctaatctata cttttccatg acttctttac ttgaagtcaa acttcaagaa
     1261 gatgactccg actttctttt tttaaaaagt attctagagg actcctttga atacacgatt
     1321 ctatttgcca aacaaaagaa gtttattaat aaagctttaa acttaaatat cgatgaaaga
     1381 aaagagttag aatctgagtt atttggcaac ttaacccaag tacaggttaa caatctaaaa
     1441 agagagataa cagctcgatt aatatcaaaa cttcaataaa actaattatt caatcagcca
     1501 actcgccaag ttatcagatt ggagagatta gttatgtcaa tacaaaagta cacaacaaaa
     1561 agcggtgcac gatatagtgt ccgcttgtat gttcgtaaag atgaaaatgg aaagcaagaa
     1621 tactatgtaa aaaaagggtt taaaactgaa aaagaagcca agttacacga agcaagaaaa
     1681 aaggtcgaga ttgaagatac cggctatcat aagccttcta atgacttgtt tataacagtg
     1741 tatgaagaat ggttgccttc ttataggaat cgtgtacaag aaacgacata tcaacgtaca
     1801 aaagaccttt tccgcttgca tatactacca aaattcggga ataagtccat ttctaagatt
     1861 actcctgttc actgtcagaa tgcagtcaat gaatgggctt taaaattcaa gaatatcaaa
     1921 cagcttaagt cttacagctc tcaaatattt gagtatgcca tttttgcaga gctattacac
     1981 cgaaatccta tggcaaatac aaatctacca aagcgggaaa agaaagaaaa tgaaaattac
     2041 ttttcattag aagaactaaa agctttttgg gatattctca ataaagaaga gcctttaaaa
     2101 catattttaa tcttccagct tttgattact actggtatac gtaaaggaga gctagctgct
     2161 cttcattggt ctgacattta ttttgaagaa cagctaatgt atgttagaaa gagctatgca
     2221 actattcgaa atgaagagaa gaatgcaaga agaaaaactg tcaggattca aaaaaataca
     2281 aaaaatatat cttccgaacg aatattacca attgatgcac aaactattga aatgttaaaa
     2341 atctggaaaa aagaacaagc tcgagagctt ttgcagtttg gcataaatac aaatacaaaa
     2401 aatcaactta tctttaccta tgtaaatgca gacggtgaca ttaatcaacc tttgcatgcg
     2461 gactattcaa ataatatatt gaaacgttta gagaagaaat ataaatttaa gcacgttact
     2521 attcatggat tccggcacac tcacgctact cttctactag aaggtggagc aagtatcaaa
     2581 gaaacacaag atcgattggg tcataaaaat gctgaaacta cccttaacac ttattctcat
     2641 gttactgaaa aagctcaacg aaatgcgtta gaaaaattca ttgcttacac tggtttataa
     2701 tattgtcatt acctttctca ttaccagaac gaaaaaaaga caccttctcg caatgagaaa
     2761 gtgtctgaaa tcgttggtat aacaacgatt aacgttttga aaactgtgaa gctttacgtg
     2821 ctttcttaag acctggtttt ttcttcgtat cattgaataa gtttgaaaag ccttgattta
     2881 taaaggtttt tccgtaaata tattttgtta tatttgatta aatttgaaat gtttgtgttc
     2941 taaaatgtgt tctaatttaa aggcagtata tttcgtattt tatattaaat ttttaggctc
     3001 attcattctc aaatccattc tatttagtca ataaatattt taaaaagact ttataaacaa
     3061 agatgttcaa gcgagcatct tatttttttg tgcctaattt gaaaggagaa catttcatgt
     3121 ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaatcaa
     3181 ttgtaggagc aattgctcct acgattgctt ttgctgatga aattacccat ccacaaacgg
     3241 taaccgtaga actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc
     3301 gtccaatgtc agaagtcact gttccgcatt atgcagtgta caacggcgtt aaacaagata
     3361 ttttctgtgt tgaaccagga gtcccgattt acaatgagtt cactcctgga tatgagaaga
     3421 acccgttacc agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag
     3481 gtacagatgc agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt
     3541 atacccttca ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg
     3601 caaaaatcaa tcaagccatt gccgattatc agaagaaacc aagcttccat aatagtacgg
     3661 ctaaaacagt attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag
     3721 agttcgatga ggttgtggaa aataccgcaa acattgatta tcgtgtgaat ggcaatcagt
     3781 tgataatcag cccaaatgcc aattcaaaag aaagtggtgt gttgacgctt aagaaatctg
     3841 ctggtaccgg aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg
     3901 cgatcgataa gccgaacact tatacggtca aaattgatgt ggaaatggag ggttctttga
     3961 agattaaaaa agttgataaa gaatcgggtg atattatacc aggaacggtt ttccatttag
     4021 actttggaaa aactttaccc gcaaaagacg tgaccaccga taaagaaggc attgcgacct
     4081 tagatgagat tcctcatggt acaaaggtaa ccattactga aaaatcggtg ccggcgcctt
     4141 atacgattga taccacaccc atgactgcca caattaaagc aggtgaaacc atttcagtga
     4201 cttcaaaaaa tacgcgagaa aaaggtcaaa ttattctgga taaaaccgga attgaaacag
     4261 ggagtgatct ttggaacgat aattactctt tagcgggcaa tacgtttgcc attcgcaagg
     4321 atagcccaac tggtgaaatc gtccaagaaa tgaccacgga tgaaaaaggt catgcagaaa
     4381 caccaaaaga gattgccaat gcgttggaat tgggaaccta ctatgtgact gaaaccaagg
     4441 ctagtcatgg ctttgtgaat actttcaaac cagtaaaagt cgaattgaat tatgtcaatc
     4501 aaacagtggc tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatt actggtgaaa
     4561 ccactttgac aaaagaagac aaagataccg gtcatgaaac tcaagggaaa gctgagttta
     4621 aaagagctga atacaccttg tttactgtta aggatggaaa agcggtcaaa tggaccgaga
     4681 actttaaacc agaattgttg aagggaacga aagcttctga tgaaaccgta actttgactt
     4741 tagatgaaaa gaatcaagtt gccgttaaac acttagcgat taatgagtat tactggcaag
     4801 aaaccaaagc gcctgaagga tataccttgg atgaaacaaa gtatcctgta tccatcaaaa
     4861 aggtggatga tagcgaaaaa aatgctgtga ttactcgaga tgttgcggca aaagaacaag
     4921 tgattcgctt tggctttgat ttctttaaat ttgctggatc aactactggc actgccgaaa
     4981 ctggcttcaa tgatctaacc tttaaagtgt cgccattgga aggaaccaat gaaatcaccg
     5041 gtgccgaaga tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca
     5101 agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt
     5161 ttcaaaagat tacgccatta gaaatccgtt ctacttttaa agaaaataaa gaggattata
     5221 ccaagagtga ttatatcttt actattaccg aaaaagggca aaagcaaccg attaaaacgg
     5281 taacggtccc ttatgggaat ctaaccaatg agaatttctc agtcagtttg aaccgtttga
     5341 tgctttatga tttgcctgag gaagaagata gcttaacttc tcttgcgact tggaaagaag
     5401 gagataaaga attgactact cttgattcta ccgagctagt cgataaattg agctataatc
     5461 tacatgaaat caaagaagac tggtatatcg tagctcaagc catcgatgta gatgctacaa
     5521 aagctgccca agaaaaagat aaaaaagcca aaccggtggc gattgcggaa acaaccgcaa
     5581 ctttagcgaa taaagagaaa acaggaactt gggagattct gcataaatta actgtcgaac
     5641 aagttttaga caagaccatt gtcttattca actatgtgta tgaaagcaaa gaagcctatg
     5701 aagctggtga tgagccggta gcgaaggatg ctagcttgaa caatcaagcg caaactgtta
     5761 attgtatggt ggaacgtcat gtttccatcc aaaccaaagc gcacttagaa gaaggttctc
     5821 agacctttac tcatggtgac gtggtagata tgtttgatga tgtatccatt acccatgatg
     5881 tactagatgg ctcaaaagaa gcttttgaaa caattctgta cgctttattt ccagatggta
     5941 caagtaaaga aatttggaaa tctggcaaga ttgattacga agtgaatgac aaagaattta
     6001 ccaaaaccgt acttgcggaa aaagtggata caagtaaata tccagaaggt acaagtttta
     6061 ccttcaaaga aattaactat gacaaagatg ggaatatcaa tggaaaacac aatgaagatt
     6121 tgaaagaaaa atctcaaact ttaacgccca aaaaagttcc gacaacacca agtacaccag
     6181 aacaatctga aatgccaact gttccaagtg gctctcaaga atctagtcct acagtgaagg
     6241 cattccctca aaccggggag aaaacttcca atgtgctact gttcattggc tttagcttga
     6301 tctttgcgac ggctggctat tatttttgga atcgtcggaa ctaaggtgat aagatgaaac
     6361 atgtaaatag tagaaaacaa tacgcgcgac cgcctcctaa aaaagtggtc gcgctatttt
     6421 tgtattcaaa actaacaatg aaaagaggaa aaaatatgga attgaaattt gttgtaccag
     6481 atatggtcga gacgttcgga aaactaacat acggtggtga aggtgaagtt ctatcggaag
     6541 ggtatggtcg aaatactaca attgttggtc gaagttacca tctgttttca aataaacaac
     6601 gggccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgaccaag
     6661 atcaaccact aaaagctgta aatccccact taattgccaa aggctatgaa attgagaatc
     6721 gtgggtttac cgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga
     6781 agatgagatt aacagaaggt attgtcgtag attcaggatt aacctttggc aagttgcgtt
     6841 tttccgcatt acgtcgagaa gtacgcaagc aaaatgagga cggaacggtt agtaacgagg
     6901 taaaagaacg gacctataat ttgaaatcct ccgcgcaagg tcgaatgatc caagtcagta
     6961 ttccagcaaa tgtaccctta cgcgagtttg cttatgatgc agaggtagag ctggtcaatc
     7021 caatcgtgga tacggtcgcc aattatgttt ttcgggaagg aaccaccgtt aattggttta
     7081 tcaaggcaga tgatttagtc ttgaaacgac aactgaatca aggaaaacct acgaaccaaa
     7141 atgaaggaaa gaaataggtg aagaatatgg aaattaatgt tgaacatatt ttagattgtc
     7201 tcgatcaatt tggcaaaggt gagctaacgg aggaacaact cactaacgca ttaacctatg
     7261 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgccaaag
     7321 aagactttga tgtactggat tggttagggg aagaagagtc tttctttatg gttgtcgagg
     7381 taaatgaaag tttatgtcgg caggcagaat ctgttttgga agaaatcgat gtggagatgc
     7441 cggatgccat cgaaagattt ttaaaacaat tagtggaaac gaaacaactt cccgtagtag
     7501 taaatgacta gcataacttt ttgaaattgc ccaatgaaat cctattgggc tttttcgact
     7561 ggaggtggaa accatcaaaa tgtacaaagg acatcgcata cgcgcaggag atcaacactt
     7621 gatttatcac tttgttcttg gctggctaat ctttttgttc attggttgga tgagcgtctt
     7681 ttatttacaa gaacttagac aatttgatat ttctaaggta tcattttcca ctagtgaaat
     7741 tggctggtct atgaaggatg taatctgctt gctaggaagt ctcgctcttt caggaagcat
     7801 gatgttactt tatatacact tttttcagga tcattggcga agtctttggc atcgacaaaa
     7861 gttagctcgg atgattctag aaaaccattg gtatgaagta aagcagactc aaggtgaagg
     7921 ttttttcaaa gatttgaata gtagttggac caaagagagt atcagttact tccccaaaat
     7981 ttattatcga atgaaagatg gcttactttc tattcgcgtt caaatttcac tgggaaaata
     8041 tcaggatcag ctattgaagt tggaaaataa gttagaaagc ggactgtatt gtgagttagt
     8101 agaaaaagaa ctcaaagact cctatgtgga atacaccttg ttatacgata tgattgccaa
     8161 tcgaattggg atagaagaaa tagtggcaga aaatggggcg ttgcgattga tgaaaaatca
     8221 agtatgggcg tatgattcct tgccgcatat gttaattgct ggcggcacag gtggtgggaa
     8281 gacctatttc ttactcacca ttattcaagc actattgaag tcagatgcag aattgtttat
     8341 cctcgatccc aaaaatgcgg acctcgccga tttaggtacg ataatgcctc acgtatactc
     8401 ccaaaaagaa gaaatttctg aatgtgtgga agatttttac gaacgcatga tagctcgtag
     8461 taggtcaatg aaagaaatgc ccaactacaa aacaggagag aattatgcgt atctcggact
     8521 tccgcccaac tttttaatct ttgatgaata cgtggcttat atgggtgcga atcgttttcc
     8581 tacaagcatc gaatagatgt gaaatggaag cctattgaga ttatcaatag tgacaactaa
     8641 tgttttgaga gatggaatga aagccttcaa gttgagctga aacatccccg ctaatactcc
     8701 tacatgctct aatcaatgaa gtagattggt tttggtatga agctaggtga agtcgtctga
     8761 aatctgccta agtgcttgag cataaggctg actatagggc gggcggctga aaaagataac
     8821 tatggtaaga atgtctgaaa atgactgacg aatctgcgaa tgtacgggtc taaaaggctg
     8881 atcttattga aagataggaa catacggacg tatggtgaga taccgacaag taaggttcca
     8941 tgatatgaaa atcgggatag ctaacgaaga agctataatt tcacaggctc atagtagata
     9001 cctaaggtta tacatgatag ctaaaaacat tggaacgatg taagcagaaa actatcaatt
     9061 aaatgactac ttctagatag gtgcatataa gtaataacga aattgctttg tttctgtgaa
     9121 agtaagggca cagtaccgat gaagcgtcta acaaatgtgg agggatagcc ctaagtctat
     9181 ttaataacaa tgaatgttga aacagatgaa gttcaaggat ctggtaagaa cgtgggaaaa
     9241 tccgttatgg aggtgccacg cttgaacaca aaaatacgtt actgggaata ttatgatcta
     9301 caagaaacat tcgatcagct atttactcaa agtagaaacg ggaaaaagtt ttatcaatta
     9361 tatgagttaa tcatttctga aaataatatt cttttagcat accgtactat caaggcaaac
     9421 aaggggtctt cgacaccagg aacggattcc ttcacaattg ataactataa ggaaatgaac
     9481 caagcagagt ttatccatct tattctcagt catttagaaa attacaaacc aaaatccata
     9541 aagcgagtaa tgatacctaa acccaatggt gagaaacgcc cattaggaat tccatgcatg
     9601 attgatcgaa tgatccaaca gatgttcaag cagatactgg agccaatatg tgaagctaag
     9661 ttttatgaac atagctacgg tttcagacca ttgagaagtg caaagcatgc tctaggtcgc
     9721 attatgtatc tcataaacat cagtaaaatg cactatgcag tagacattga tatcaaaggt
     9781 ttctttgata atgtaaacca ccgtttactg ataaaacaat tatggaatat aggaatttgt
     9841 gacaaacaag tcttagctat tctttcaaaa tctctgaaat cacccattca aggagaaggt
     9901 atcccgagca aaggaacgat ccaaggaggt atcatttctc cactattatc taatgttgtt
     9961 ctaaacgact tagaccattg ggtctcaaag cagtggcata cctttgaaac aaagtatccc
    10021 tatactaaag gttacaataa atttcgtgct ctaagagata caaatctaaa gcaaggctat
    10081 attgtcaggt acgcagatga ctttaaaatc atgactaatg attatccgac agcattaaaa
    10141 tggttccacg ctgtcaagct ttacctaaaa gacagactta aattagatat ttccaatgag
    10201 aagtctaaaa tcgtcaattt gcgtaaatgt aagtcggaat ttttaggatt cgctatttgc
    10261 gtgaaacaaa aagggaaaaa atgggtttgt aattcacgta tttctaacaa aaaaaaggat
    10321 cagattaaag aagagattaa acaaagaatc aaagatattc aaaagagtcc tacagcacaa
    10381 aatgctctcc tgtttaatgc tctcgtgctc ggtgttcata attatttcag gtatgccact
    10441 catattaatt tagatttgaa taggatgaac tacgttcttt cattcactac ttacaaccga
    10501 ttatcaaatt gtagtacaag atgttacccc aacaaagctt ccccaactta caaaaagttt
    10561 tactcaacaa cgatgaaaac gtataaagta gctggagtct atttgtaccc tctttgcgac
    10621 gtatctacaa aaacaattta cggatttaat acggaggata caccgtttac tccgtttggt
    10681 cgtcaaagat tggagcacaa aagcctacag tctcttgtgt tccaagaatt aagaaaattg
    10741 atggaatcga aaataccgaa tagaacggtt gagtatttag atagtagaat atctcgttac
    10801 tcaatgaaat caggcaagtg tgagattaca aaacaatttt tgccagcaaa agccgttcac
    10861 tgtcatcact atttgccaaa aagtcttggt ggagacgaca aattcgataa cttgagaatt
    10921 attcataagg atatccatct tctgatccat acaacaaata aaatgataat cgatcactac
    10981 gtgcatgaac tgaaactggt gccagagcaa atagcaaaaa tcaaccttta tcgaaagatg
    11041 tgtaatctgc aaaacattca atagaaaata aatagatgga acgccgtatg aggtgaaagt
    11101 ctcacgtacg gtgtggagcg ggggaaaaga tggagattat atcaaattct tacctatcgc
    11161 tatagatgtt aacgacaaaa gaaagtgcgg tgattttgaa taagcttaaa caaatcgtga
    11221 tgttaggccg tcaatctggt ttctttctga ttctagcttg tcaaaggccg gatgcgaaat
    11281 atctaggaga tgggattcgc gatcagttta acttccgtgt ggctttgggt cgtatgagtg
    11341 agcttgggta ttccatgatg tttggagaag tcgataaaaa tttttttatg aaacgcatca
    11401 aaggacgagg ttatgtagat acagggggca gtgtgattag tgagttttat acgccacttg
    11461 taccaaaagg gtatgatttc ttaagggaaa ttagcaacat tgttggatta accgttcata
    11521 cggaaagaga aaataatagt gtgtagccta ggaatatcaa taatcctggg cttttttatt
    11581 gaggtgattt atgacatttt tagatttatt cgccgggatt ggtggctttc gcttaggtat
    11641 ggaacaagcc ggtcaccagt gtatcggttt ttgtgaaatt gatgaatttg ctcggaggag
    11701 ttataaagca atccatgata caaggaagga ggtggaaatg catgacatca caagtgtatc
    11761 agacgaattt gttcgatccc tcggaccagt ggacatcctt tgcggcggct ttccgtgcca
    11821 agctttttca attgcgggaa agcggcaagg attttccgat actcgaggta ctttattctt
    11881 tgaaatcgct cggttcgcca ctcttctcca acctaagttt ctattccttg agaacgtccg
    11941 gggattgctc aatcacgaag gaggggctac gttcgagacg atcctccgaa cgttggatgg
    12001 attggggtat gatgtggaat ggcaagtgct taactcaaag gcctacgttc cccaaagccg
    12061 tgagcggatc ttccttatcg gacattctcg agatgcatgt accgaacaag tatttcctat
    12121 cattggatcg tcttcaacat ctgatcaaaa catcagaaac ttggtaaata tcaatccttc
    12181 caatcgagga atgggtggcc aagtctatgg ttcagatagc gtagcaccaa ccttgaccgg
    12241 tgatgaagga attaaaattg ccttaccggt aaaggatggg atttctgtag cgggtatgtt
    12301 gtcgggaaat tttgaacaag gcaataggat ttatgaggta accggaacag cccccacttt
    12361 gtcaacgaaa caagggggaa cgaagattat gattcggaaa aatgaaacag aatatcaaga
    12421 ggtgaagcca ggggacagtg tgaacctggc ttttcctaat tccacaagtc gccgaggtcg
    12481 tctgggaaaa caaagtgtgc ataccttact aacaggagac caacaagcgg tagtcacgga
    12541 tcagtaccag attcgaaagt taacacctag ggagtgctgg cggcttcagg gctttccaga
    12601 ttgggctttt gatcgagctt ctcaagtcaa ttcagatagt cagttgtata aacaggccgg
    12661 aaactcagta acagttccgg tcatttttga tattgctaga agattaaagg gggtaaaagc
    12721 atatgattta tgacgagttg attggagaaa tctactggat gattggcaag attcaggcag
    12781 atccgaaatt ggaagaagaa ctgtaccggc tcaattttga tattcgcaaa aacggggtga
    12841 aagtccaggt gattcgtatg tagtggatga ggaaacggat gctcgaatgg agctcaatca
    12901 agtcatcggt gaattggaac gaattgcgga ccttgccaaa gaacctgata tccgccaata
    12961 cttgtttgag ataaaagcag agttggaaat tgagggtatc accgcggagt aatagggaaa
    13021 taacccactg ataggcactg accgcggggg atttgcgaag cagaaatcct ccgtggtgct
    13081 gtgcctgttc atggacggcg gcagccggcc atgatgtctc accccccgta tctaacaggg
    13141 gggtacaaaa acacaaaaaa caagcaagta accataaaaa atgcttgttg tatcaaggat
    13201 attaagtaat ttgtataggt gttaactcat ttggaaaagt taacaccttg aatttggagg
    13261 tgaaaacttg gcgcaacgaa atttagatta tcggttatta aaagaacgac gcaacgaata
    13321 tggcgtttca caaaataaat tagctactgc ttgcggactt agccgcccat acctaaatca
    13381 gatagaaaac ggtggcgtaa tcgcctctac aaagaccatg agaaaaattt ttgatcaact
    13441 ggaaagtttc aatcccgatt tacctttaac gttactattt gactatgtaa ggattcgctt
    13501 tcccacaacg gatgtgcgga aaattattca agaaattctc catttgaaat tcgagtatat
    13561 gcttcatgaa gattacgcct tttactccta tcaagaacaa tatgtcatgg gcgatattgt
    13621 agtgatgtta tctcatgaag aagataaagg tgtccttttg gaattaaaag gtcgtggttg
    13681 ccggcagttt gaaacttttt tactcgccca aaagcgtagc tggtacgact tttttgaaga
    13741 ttgtctaaaa gccggtggcg tgatgaaacg cttggattta gcaatcaatg atcgagtggg
    13801 gctactggat attcctgatt taacgaaaaa atgtcagaag gaagaatgta tctccttgtt
    13861 tcgtaccttc aaaagttacc gttccgggga actgttgaaa gctgatgaaa aggatggtat
    13921 gggcaatacc ttatatattg gcagcctcaa aagcgaggtt tatttttgtt tgtacgaaaa
    13981 agattatgaa caatatatca agttagggat tccattggac cagaccgaaa cgaaaaatcg
    14041 ttttgagatt cggttgaaaa atgaccgtgc ttattatgcg attcaagatt tgttaaaagg
    14101 tcgtagcatt gaaagtacca cgttttccat tatcaatcgg tatttgcgat ttgcggataa
    14161 agtagatgga aaacggcgaa ccaattggcc cttgaatgaa caatggggtc gttttattgg
    14221 acggaatcgg aaagagattc aattaacttc tgaaccgaaa ccgtatacca ttgaacgaac
    14281 attaaattgg ctaggtagac aagtcgcccc tacgtggaaa atggctaagg agttggatcg
    14341 attgaatcaa acaacgtata tccaggatat ggtacagaat gcgcgactct cggaccggca
    14401 taaaaagatt ttggagcaac aaagtatggc aatcgaaaac ttgattgtat gaaaagagga
    14461 attttgatgg aaattgtaaa agatatgtta ttggtacttg gtggcagtat gcttggtgtt
    14521 accgtgatgt gcttgatgaa tgcaagtgca gcagcggatt gagcaatgga aaagaaagga
    14581 aagtgacaaa atgaatttcg gacaaaattt
//
LOCUS       DAZUKT010000066        14440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000066 DAZUKT010000000
VERSION     DAZUKT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.067"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..1113)
                     /locus_tag="ACG816_002114"
     CDS             complement(226..1113)
                     /locus_tag="ACG816_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4920972.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="ACG816_002115"
     CDS             complement(1110..1979)
                     /locus_tag="ACG816_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4920973.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="ACG816_002116"
     CDS             complement(2116..3402)
                     /locus_tag="ACG816_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4920974.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..4253
                     /locus_tag="ACG816_002117"
     CDS             3675..4253
                     /locus_tag="ACG816_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4920975.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4253..5182
                     /locus_tag="ACG816_002118"
     CDS             4253..5182
                     /locus_tag="ACG816_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4920976.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5179..6915
                     /locus_tag="ACG816_002119"
     CDS             5179..6915
                     /locus_tag="ACG816_002119"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4920977.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="ACG816_002120"
     CDS             complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="ACG816_002120"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4920978.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7811..8320)
                     /locus_tag="ACG816_002121"
     CDS             complement(7811..8320)
                     /locus_tag="ACG816_002121"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4920979.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="ACG816_002122"
     CDS             complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="ACG816_002122"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4920980.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9621..10712)
                     /locus_tag="ACG816_002123"
     CDS             complement(9621..10712)
                     /locus_tag="ACG816_002123"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4920981.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="ACG816_002124"
     CDS             complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="ACG816_002124"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4920982.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="ACG816_002125"
     CDS             complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="ACG816_002125"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4920983.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="ACG816_002126"
     CDS             complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="ACG816_002126"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4920984.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4113 a         3144 c         2609 g         4574 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
     3901 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
     3961 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
     4021 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
     4081 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
     4141 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
     4201 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
     4261 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
     4321 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
     4381 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
     4441 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
     4501 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
     4561 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
     4621 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
     4681 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
     4741 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
     4801 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
     4861 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
     4921 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
     4981 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
     5041 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
     5101 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
     5161 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
     5221 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
     5281 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
     5341 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
     5401 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
     5461 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
     5521 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
     5581 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
     5641 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
     5701 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
     5761 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
     5821 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
     5881 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
     5941 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
     6001 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
     6061 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
     6121 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
     6181 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
     6241 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
     6301 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
     6361 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
     6421 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
     6481 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
     6541 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
     6601 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
     6661 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
     6721 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
     6781 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
     6841 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
     6901 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
     6961 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
     7021 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
     7081 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
     7141 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
     7201 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
     7261 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
     7321 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
     7381 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
     7441 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
     7501 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
     7561 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
     7621 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
     7681 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
     7741 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
     7801 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
     7861 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
     7921 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
     7981 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
     8041 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
     8101 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
     8161 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
     8221 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
     8281 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
     8341 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
     8401 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
     8461 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
     8521 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
     8581 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
     8641 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
     8701 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
     8761 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
     8821 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
     8881 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
     8941 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
     9001 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
     9061 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
     9121 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
     9181 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
     9241 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
     9301 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
     9361 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
     9421 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
     9481 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
     9541 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
     9601 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
     9661 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
     9721 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
     9781 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
     9841 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
     9901 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
     9961 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    10021 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    10081 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    10141 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    10201 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    10261 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    10321 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    10381 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    10441 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    10501 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    10561 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    10621 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    10681 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    10741 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    10801 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    10861 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    10921 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    10981 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    11041 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    11101 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    11161 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    11221 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    11281 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    11341 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    11401 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    11461 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    11521 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    11581 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    11641 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    11701 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    11761 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    11821 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    11881 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    11941 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    12001 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    12061 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    12121 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    12181 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    12241 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    12301 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    12361 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    12421 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    12481 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    12541 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    12601 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    12661 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    12721 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    12781 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    12841 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    12901 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    12961 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    13021 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    13081 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    13141 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    13201 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    13261 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    13321 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    13381 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    13441 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    13501 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    13561 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    13621 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    13681 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    13741 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    13801 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    13861 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    13921 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    13981 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    14041 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    14101 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    14161 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    14221 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    14281 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    14341 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    14401 gttttctaga tttcataaaa aatcgctcca aactgatgta
//
LOCUS       DAZUKT010000067        14275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000067 DAZUKT010000000
VERSION     DAZUKT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.068"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG816_002127"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG816_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4920985.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG816_002128"
     CDS             complement(323..892)
                     /locus_tag="ACG816_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4920986.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG816_002129"
     CDS             1078..1980
                     /locus_tag="ACG816_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4920987.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(1992..3437)
                     /locus_tag="ACG816_002130"
     CDS             complement(1992..3437)
                     /locus_tag="ACG816_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFS4920988.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(3557..5341)
                     /gene="pyk"
                     /locus_tag="ACG816_002131"
                     /pseudo
     CDS             complement(3557..5341)
                     /gene="pyk"
                     /locus_tag="ACG816_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
     gene            complement(5415..6377)
                     /gene="pfkA"
                     /locus_tag="ACG816_002132"
     CDS             complement(5415..6377)
                     /gene="pfkA"
                     /locus_tag="ACG816_002132"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4920989.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(6545..9859)
                     /gene="dnaE"
                     /locus_tag="ACG816_002133"
     CDS             complement(6545..9859)
                     /gene="dnaE"
                     /locus_tag="ACG816_002133"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4920990.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            10090..10275
                     /locus_tag="ACG816_002134"
     CDS             10090..10275
                     /locus_tag="ACG816_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4920991.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(10337..11533)
                     /locus_tag="ACG816_002135"
     CDS             complement(10337..11533)
                     /locus_tag="ACG816_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4920992.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(11703..13139)
                     /locus_tag="ACG816_002136"
     CDS             complement(11703..13139)
                     /locus_tag="ACG816_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4920993.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            13432..14241
                     /locus_tag="ACG816_002137"
     CDS             13432..14241
                     /locus_tag="ACG816_002137"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4920994.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4283 a         2945 c         2515 g         4532 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtcagattgt agacaaaaag gagttggtaa ttaagccact cctttttgtt
     2041 agtctttgca taattttgaa ataaaaagcc ataggctttt ttgttttcca tagccatgtg
     2101 gctagttttt ttaaattcat ggcagcaaac gtaagcatcg tgtgcgtggc gactttttca
     2161 agacctcgat atttcgtcca acgcatacca tgcttttctt ttgcatctgc aaatattcgt
     2221 tcaatggttt gttttctctt tttgtaaatt tctctattca acgccgtatg gcgttggtgt
     2281 tcaagttcat ccataagatc agcccaaata tgtcgagtca ctattttttg atgttttgta
     2341 ctcaaagtac aattttcaag taatggacaa ctcacacaga attttggatt tgatttatag
     2401 gttcgataac catcacgagt gatagttgaa aataacaacg gttgattatt gggacataaa
     2461 tagcagtcaa aataggcatc ataaacaaag tcttttttac gaaacattcc tttcactcca
     2521 tgaggtgcag tatatggaag aatcgggtag agtttcaagt gtgacagaaa atgaacaaat
     2581 ttaggcgtct tatatcctgc atcagcaacc acacgcttta tatttggaaa agctcttttc
     2641 gactgtttaa ccagatcgat tgcgacttgg ctgtcatgca catttcctgg tgtgattaat
     2701 gtagataaga taaaaccgtt atcttcacag gaagtatgtg cagaataagc aaattgtttt
     2761 tctcgttcac ctttaacata gtaaccactt tctggatctg aatgacttat tttttgaaga
     2821 ctcaactcac tttctgtgtc ccatgtaaaa ggacgttttc ctacagcaat acgttcttta
     2881 tttatttcat cttctaattc atcttgataa gcttttgctt gaccgtaggt catctctttc
     2941 gtgaacttat gtttattggc atttgctttg atatgagtag aatcaatata aaggttgtct
     3001 tccgtaacga agccagcttt tacagcttgt tctagaatat gagagaaaat atcttcaaat
     3061 aaagttgttt ctctaaatct tcgaacatag tttttaccaa aagtagagaa gtgtgggata
     3121 ggatcctcga atgaataacc taaaaaccaa cggtaagcaa catttgtttc aacgtcttta
     3181 attgtctgtc tcatcgaacg gataccaaat aaatattgga taaatactaa tttaattaag
     3241 accactggat caatactggg acgaccatag gttgaatacg tagattctac aatgggataa
     3301 atgaaatcaa actgaatcgc agcatcaatt tttcgaacta agtgatcttt aggtacaaga
     3361 tcatcaattg ttaacatagc aaattgattt ctaccttcaa tcgattgttt tctcatcatt
     3421 agaatcactc ccagaatagt ttatacaatt agtataaaac aaaaaactgc aaacaacact
     3481 ccaaatttag gagtttgtct acagtctgac agctcttcgt aaaagaagag ctgtttttat
     3541 tttttattaa gctaaattag atagctgttg tttcgccacg gtaaacgatg ccgcgacgtg
     3601 aatcaacggt aatcaattca ccttctgtaa ttgttgttgt tgcgccttta gcaccaacga
     3661 ttacaggaat atcttgtgca attcctacaa ctgcagcgtg agaagtcaat ccgccttctt
     3721 caacgataac agcagctgct ttttcgattg caggcatata ttctttgtca gttgatggta
     3781 caactagaac cattccgtct tttgcttttt ttacagcttc ttcagcagat gaagcaataa
     3841 ctgcatttgc aacgacagat ttttgtccaa cgccttgagc ttctaataat ttagaaccaa
     3901 tcatttgaat tttcatcacg ttagttgttc cgcgttaacc aactggtaca ccagcagtga
     3961 tcaagattaa atcgccttct gatgcgaaac ctaattcaac agctttttta gctgctagtt
     4021 caaacatttc atcagttgtt gatggttttt cagcaactgt tgggtaaacg ccccagttta
     4081 acatcaaacc acgttttgtg cgttcgtcaa aagtaacagc caagatatct gcatctggac
     4141 gatattttga aatcattttt gcagtgtatc ctgattcagt tgcagcaacg attgttttca
     4201 cgcctagatt ttttgcagca cgagctacag ataaaccgat tgtttcagtt acatctgttt
     4261 tatcgaattc gttcaattgg aatgtaccta attctgataa tgcattttca gcttcttggt
     4321 cgatacgtgc cattgttgaa acagcttcaa ctgggtagtc cccgtttgca gattcaccag
     4381 atagcattgt tgcatctgtg ccatcaaata cagcgttagc aacgtcagaa gcttccgcac
     4441 gtgttggacg tgggttttct tgcattgatt ctaacatttg tgtagcagtg ataactggga
     4501 taccagcagc attacatttt ttgataatgc gtttttgaac cattggtact agttcagctg
     4561 ggatttcaac acccatatct ccgcgggcaa tcataacacc gtcagaaact ttgatgattt
     4621 cgtcaatgtt gtcgatacct tcttgagatt cgattttagg gaagatttga acatgagtca
     4681 tgtctttttc ttctaagatt tcgcggatat ctagaacgtc ttgtgcttta cgtacaaaac
     4741 ttgcagcgat aaagtcgata tcgttgtcta aaccaaaacg aatatcgtca gcatcttttg
     4801 gagtaatacc tggcaaacta atagaaacgc ctggtgcatt taccccttta cgtgatccta
     4861 gcatacctgc atttttaact tctacgacta attcacggtt tgcttcgtct ttttcaatga
     4921 tttgcatatc gatcaaacca tcatcaaata aaatgtgtcc gccaacgtga acatcgtcaa
     4981 ataatcctgg gtaagtaacc gcgatttttt cttttgaacc tacatgttca ggatccatag
     5041 agatgcgtgt ttgatctcct acttcaaatt tgatgtatcc tgcacgacca aaatcagctt
     5101 ctgttgtgcc ttgaacagtt gtacggattt cagctccttt tgtatctaaa agaataccga
     5161 catcttttcc tgtcttttta actgcttcac ggaccatagt catacgtgat aattgttctt
     5221 catggtcacc atgagaaaaa ttaaagcgga agacatttgc cccagattca atcaattgag
     5281 aaataatttc tactgagtta cttgctggtc ctaatgtact aacgattttg gtttttttca
     5341 tttaaaaaac tctcccattc aattttgttt atttaaagct gtacacagct taaatagctt
     5401 ggtttctttc actcttaaaa tgaaagtgaa ttatttagat catatagtga taagtctggt
     5461 ttatgtttgt tgttttcaag tgtatcaatg atgtcagacg caacgatttc gttgtcatgg
     5521 ataccgacac ataatccacc ttttccttct tgaagcaatt cgacagcgta agcaccgaat
     5581 ttacttgcca acacgcggtc gcgtgcgctt ggtgctccac cacggacaac gtgtcccaag
     5641 atagatacac gtgtatgata gtcaccaaat tctgaaagtt tctcagcgaa ttcatttccg
     5701 cccattacac cttcagccaa gatgatcaag caatgtttct tcccgcgatc gcggccttct
     5761 tggatttttt tagcaactga agccatatca aaatcatgtt ctgggatgat gatttcatcc
     5821 gctccgccag ccacacctga ccataaagcg atgtctcctg cattgcgtcc cattacttcg
     5881 ataacgaatg tacgaacatg agaagtcgct gtgtcacgaa tacggtcaat tgattctagt
     5941 actgtattga ttgctgtatc aaaaccgatt gtgaaatcag tcccaggaat atcattatcg
     6001 attgttccag gaatacctac tgctgggaaa ccgcgttttg tcaaagccat tgcgccatga
     6061 taagagccgt ctcctccgat aaccactaga ccttcgattc cgaatttttt caattgttcg
     6121 attcctttta attgaccttc ttcagttgca aattctggat aacgtgcaga atacaagaat
     6181 gtaccaccac gttggatctt gtcacctaca tctgccacat caagacgacg aatatctcca
     6241 gcaactaaac cggcaaaacc ataattgatc ccgtatactt ccagtccttc gtaaatacct
     6301 ttacgaacaa cggcacgaac agctgcattc attccaggtg cgtctccgcc actggtcaaa
     6361 attccgatgc gtttcataat aaacattcac ctcattaaaa tactttatcc taagtatgac
     6421 ttaggtttac aaccattttt gcgttaacca cgactaacat tctaacatta tttttctcac
     6481 ttgtcttgat ttacggataa aaaaatgaaa aaaaatgaaa gcaagtagta ttttttaccg
     6541 taagttattt gaaaaccaca ttatcttcgc caagcagata aataagttgc ccctttatac
     6601 tcttgctgtc atccacccaa taagcatctg acagaactac ttttcgttgc gttttttcgt
     6661 gatacataac tactggtata tatcctgggt gtttctgaaa aatcacttgt aattgtttca
     6721 ttatctcggg atcttcccgt tcttctgtaa tacgcaaata gcaagtttgt gaaggatact
     6781 gggcttctaa ctggttagga tcagcaaagc tttctccaat tagttgcagt tcctgattat
     6841 atttgctgat ttcgctccgc ccttgaacaa agatcactcg atcaacggca aacaaattac
     6901 gatatttacg ataaagagtt ggaaaaactg tgacagaaat ttctccacta ctgtcggttc
     6961 cttcaacaaa ggccatcagt tctccttttt ttgtccgaat ttctctaact tccttgatca
     7021 tcactaataa attgactggt tgattaggga cgatatctgt aatcagctga acttgtttag
     7081 ctaaacgaag acgatcaaac ccttctgcag gatgacctga caaataaata cctaagtact
     7141 cctcttccag cttcaagcgt tcttctagtg tatagtcagc gacttcttct tcttttaatg
     7201 ccatgattcc cagcaagtca agacttccac cactgtaaac gatattttgg atttttcctt
     7261 cgagatcaag catcaattgt ttcctgttag gagataattc atcaaaaact cctactgctg
     7321 tcagtgattg cagtaactct aatttcagcc aacgcttatc gatccgtatg aggaattgat
     7381 ccactgactg gaacgggcca ttttgttttc tttcgtttaa tatatcttct acaaaatcac
     7441 gccgaatacc ttttatcgct gtcagtccga atctgatttc ttccttattg accaactcaa
     7501 aactggaatt actttgatta atggacggcg aggaaagttt gagtttattt ttacgtgctt
     7561 ctgcaatata ttctttcaat tttgatgtgt tatgccgaac agaattcatc aggctggtaa
     7621 agaaagcacc tggatgatgg gcttttagat acgccatttg gaatcccacg aatgaataag
     7681 caaatgcatg tgaccgattg aatccatagt ttgcaaattt ttcgatatat tcatatacgg
     7741 tcgctgctgt tttttccgaa tatccctgtt gtaatgctcc tgatacaaaa tgcgactttt
     7801 cttcatctaa tacgtctttt ttctttttac tcactgcccg tcgcagaata tctgactgcc
     7861 ctaatgtaaa tccagccata gtcgaggcga tctgcatgat ttgttcctga tagacgatca
     7921 ctccatatgt gtttttcaaa atcgggatca gtgaatcatc tgggtagcga atggcttctt
     7981 ttcctttctt cctggcaatg aaggtatcta tattttgcat agggcctggt cgatacaatg
     8041 cattgactgc ggcaatatct tcgatgtttt ctggatctaa tcttcgtaaa acgttccgga
     8101 taccagcaga ttcaaattga aatactcctg aggtttcgcc tcgttgaaac aagagcagcg
     8161 tttttgcatc atttagcggg atctctttta atcggatctc atacccttct actcgcttaa
     8221 cagctttcaa agtaaaatca ataatcgata agttgcgtag tcctaaaaag tccattttca
     8281 aaagaccgat tgcttctacc tcattcatcg tatactgggt cagaaaagct tcatcagaac
     8341 cgcgttggag cggaactagt tcaagtagat tcttatcgct gataactaca cctgctgcgt
     8401 gagtcgaaac atgtcttggc aatccttcta aaatcactgc agttttgaat aaaagttggt
     8461 ttttttcgct tgcctgaact aattctacta attgatgcga agttttataa gcttgggtca
     8521 atgtgatttt caattgattg ggtattgctt ttgaccagcg gtttgcttca ctttgcgata
     8581 aaccaaacac tctagctgca tctctaagaa ccatttttgc tgccattgtg ccaaatgttg
     8641 cgatttgtgc catatgatat tgtccatact tttctcgaac ataattcaat acttcttcac
     8701 gtcgattgtc aggaatatcc aaatcgatat ctggcatcgt aaagcgttcg gggtttaaaa
     8761 agcgttcgaa cagcaaatca tattctaatg gatccacatc tgtgatttct aacacatagg
     8821 ctactaatga acccgcagca gaaccacgac ctgcaccggt aacgattttg tttctatgag
     8881 caaaagccat tacatcccat acgatcaaga aataatcatc gaatcccatt tgatggatga
     8941 cagataactc gtagtccaaa cgttcttggt attcctttgt atagtcagtg attcggcgtt
     9001 ccacttgcga atagcataac tctcttaaat attctccagc ttgttttcca ttaggaactg
     9061 gataatgcgg aagtaatttc tgatggaact ggaattcaaa tgaaatcgat tcagctaaac
     9121 gctgggcgcc tattacagct tgggaaagac ccgctttttc gaatcgtttg atcatttctt
     9181 gttctgaaag aagatagaca tttgctgtta gtggtaaaga tgcagataaa ttttctaaag
     9241 gtatttggct tccctctttg atatgtttca tcacttcaag tgcaaaaagt tcttcttgtt
     9301 gtaaactagc gatgggatgt aatgcacaaa gcgaataatt ttcttttatc acatactgat
     9361 accattcagg caattgatca ggcgtgtttt ctgtcaagcc gatataaaaa tttctagtaa
     9421 aaacattttc aagtaaaaac tgccaatgac cgaaaaagtt atcctcttta ttgaaagcct
     9481 cagaaacaga atgatttgcc ggaacaacaa acgctagatg gtcggtttct gtcatttctg
     9541 caagttccaa tttttcttgg atcatttttc ggctggaaag tttcatcagc tgctgataac
     9601 ctatttcatc ttttgcgtac gcaagcaagc cataattttt ttcttcataa gtataactaa
     9661 gatgtaatcc aatgattggc tgtactcctt cttctacaca aatacgatag aattcaggtg
     9721 caccgtataa gacatcttta tccgttatgg ctaatgaagt gtagccttgt ttttttgctt
     9781 cttgtacata ttctcgaata cgtatcgtac tttgtaaaag agaataagaa gtaattgtat
     9841 aaagttgtgg gaaaaacatc ttgcattccg cctttttttt cgttaaaatt cactagcgtt
     9901 aaaaggtttg atccttttac tttcattaag atagcaggcg ctgaagttga ttattccact
     9961 tcatttgctt gcttgtaatc tgtaggaatg agttctttcg caagaactca aattttattg
    10021 agatttggtc tcgtttcttt acattcaaag aaaatctggt aaaattaaga tagaaaagag
    10081 gtgttcttta tgcgatatat cgttacatta ttctgggctg tcgttctagg tcaagtagtc
    10141 ggttatattg gtgctgcatt aacaagcgga acatatgatt ttactttgac aacaatcatt
    10201 tcattcattg cgggtgtcat tatccttttg attggtgcag ttgcgccaag aaaagaaacc
    10261 tctgcccatt cgtaaaaaaa cagccaagag cttgagattt ctcaagctct tttttatttt
    10321 ctactcctca ccaccattat aatctgctgc gaagaacttt acgaaagttg aataaagaaa
    10381 gaagaatatt tgacagaaca aacaaacttg tcacaataaa aacagcactg taattataag
    10441 catgcgcgac tgtcgatcct agcatgggac ctaaaacttg tccaaagttg ctaaacatct
    10501 gattgtagct atagattcgg ctgactcctt cttgaggcgt aatcttactg atcagtgtat
    10561 tgacagaagg catcaaagcg ccagttgaaa agcctaaaat aaagcgaagt atacctaatt
    10621 ggagcggtgt agtgacgaat gccataggta aataacagca aaatgataaa atgagtcctc
    10681 caattagaac tttttgattc ccaatcttat cccctagttt tcctagtttc ggtgaagaaa
    10741 agacagcgga aacaccagct atcgaaacaa tcaagccgct gacaaaaagg atattttctg
    10801 tacttccgct caattcacga atatataagg tcaaaatagg gctgatcgta gtgattccta
    10861 tctgtaaaat caaggtcgta atgaagagac cgatcaaaat agagagatga tccatttttg
    10921 aaaaaatttc tttggtgctt atcagatctt ttttctcgat tggatggaaa tcttctttga
    10981 ctaagaaaat ggtcaatacc gttgtgatca tcaacaaagc ccctgtgatc aagaatacat
    11041 tttccatacc aaaccactga gccagtgcac cgcccattga aggtccgatc agatttccag
    11101 caatcgctcc ggtagacaat gttcctaacg cccatccgct cttttctcta ggtgcctgtg
    11161 aagcaatcat cgctgtggca tttggaatat atccagacaa aatcccattc caaaaccgca
    11221 tgatcaaaag ccaatacgcg tttggtacaa aagccaaaga tcccattgtt aatgtcatcc
    11281 ctgctgctgc acggatcatc atgagttttc ggccttttcg atcagctaga tttccccaga
    11341 ttggtgcaac cacggcagat gcaaaagcag tcacagagat tgctagtccc gaaaacaatt
    11401 cgatttgatc ttttggtgtt cctagttgct caacataaac tggtatgaat ggcataacta
    11461 aactaatact tgcaccagtg aaaaaacagc caatccacgc aatcactaaa ttttttctcc
    11521 aattaatctc catctatcat ccctctttcg tctcttttat catacctatt ttcgaataaa
    11581 caagcaacga tctcgccagt aaaaagagaa aaatcccgca aaggtcattt tatatgtaac
    11641 gttcttttta tgtaaaacaa aaagagatca gaacaaatac tgttccaatc ccttctcttt
    11701 agttaaattg ttgctagaat gatgaagttg ataatgaata aaatatcaac gatccacaaa
    11761 gcaacagaaa cttgattgta ttttcctttg attactttca caattgtata gaaaataaat
    11821 cctgctgcaa tcccatagga aatgctatag cacaatccca taaagatcga tgcaaagaag
    11881 gccggtaaag cttcttccat atctagccat ttgatgtctg caaaagaagc catcatcatt
    11941 actccaacta aaatcaatgc tggcgcagtt gcttgatttg gtacaatggc gatcagtgga
    12001 gagaataaac tacttaatgc gaatagcact gccactacta ctgaagtcaa accagtacgt
    12061 ccaccggccc cgatcccagc tgcagattcg acataagttg ttgtattcga tgtaccaaag
    12121 atagccccca cagaagtagc gatcgcatcg gcaaatagtg ctttatccat ttttgttttg
    12181 aacccacgac catcttccaa cgcgatctcg tcttcttttg agaaaatacc tgtgcggcgt
    12241 cctgtaccga taaatgttcc gatcgtatcg aaagtatcag ataaactgaa tgcgatgatc
    12301 gtcatcaata cttgaggaat cttggaagaa tcactgaata aagattgcat gccttcagaa
    12361 ccaaatgctg caccgaaagt tgttcctaat tctttaaatg aattacctaa cgaattcgtt
    12421 tgccaatcaa tggaacttaa atcaacaaca cccataaaaa taccaaggac tgtggtcgct
    12481 acgataccga taagaatagc cccacggaca ttttttacaa ctaaaatggc tgtcagtacc
    12541 aaaccaatca cagccaataa gataggagcg ttgtcaaagt tggctaacgc agggacgatt
    12601 ccaccattta tcgtaacatt tgtcgctttt ccgccttcaa cgacagagct cgaaatagct
    12661 gactggtctg ctgaaaaaga taagaatcct gcatttttga ttccgacata cgcaacgaaa
    12721 ataccgatcc cgccaccaat cgcatgttga agactttcgg gaatcgcccg aataatcatt
    12781 tttcgaatat ttgtgacagt aataaaaata ttgattaatc cgcaaataaa caccattgcc
    12841 aaggcttgtt gccaagaata accaagccca aaaacgaccg taaaagtgaa aaatgcgttc
    12901 aatcccatac caggtgcctg agcatatggt acatttgcga acaatcccat taccagcgta
    12961 ccaataatcg atgcaatgat tgtcgctaga aatactgctt ggaagggcat acctgatgct
    13021 gacaaaatcg tcggatttac gaataagata taactcatgg caaagaatgt cgttactccc
    13081 gccattattt cagttgatac atttgtattg tttttcttta aaccgaaaaa cttttccata
    13141 aaaagttcca ctccctgaat ttttttctga tggaaaacta taataataaa aaataagtaa
    13201 aatagggcaa atccatcgaa caatttcaaa ttatatagac gtttcattcg cttttcaacg
    13261 tttttttaac aaaaaaagcg aaaataatac ttaacgttcg tgtttagctt atgtttagct
    13321 atttatatag ccataactgt agtttttgtt aggaatagct gattgctctt gttaattacc
    13381 taaaatatgc tagactaaaa caaatgctag ttgaggaagg aaggaccctt attgaataaa
    13441 aaattaattg ccatcgatct ggacggaact accctcaatc aagattcgat gattacatct
    13501 aaaacaaaag agactctaaa aaaagcaatc aatgccggtc atcatgtcag tattgctaca
    13561 ggacgcccat tccgaatgag ccatcagttt taccaacaat tagaattaac aacaccgatg
    13621 gttaatttta acggcgcatt agtacacatc cctaatcagc actgggatgg tgaaagagaa
    13681 acgttgatca atcgtgaaat cgcttttgaa atcttatcgc aaaaaaagca gctaaattta
    13741 gattttatcg ctgctgaaaa tcgtgatact ttttttattg acagttttga tttctttgat
    13801 gaaaaaattt ttgcttcttc tcgtccagga gagaagaatt tgctttctcc taaaaatttg
    13861 acgacaaatc ctacatcttt gcttgtccga acagacaaac gttttgcagg agctgtatct
    13921 gctgaattaa cacgtcaatt tggcagttat gtcgatgttc ggacatgggg tggacctact
    13981 gccatcttag agattgtttc aaaaggaatc caaaaagcaa aaggcgtgca agagattgct
    14041 aactacctgt ctatcgatca aaaagacgtc attgcctttg gtgacgagca taatgatttg
    14101 gaattgttgg attatgcagg ttggggagta gctatggcta acggtactga ccaactcaaa
    14161 ggcattgcaa atgatgtcac tcctttgagc aatcaggaag acggtttagc tgtttatctg
    14221 gaaaaactac ttaaactata atagagaatt agattccaca aaaaagaggt tgtga
//
LOCUS       DAZUKT010000068        14214 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000068 DAZUKT010000000
VERSION     DAZUKT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.069"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..499)
                     /locus_tag="ACG816_002138"
     CDS             complement(29..499)
                     /locus_tag="ACG816_002138"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4920995.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(658..1116)
                     /locus_tag="ACG816_002139"
     CDS             complement(658..1116)
                     /locus_tag="ACG816_002139"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4920996.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1287..2432)
                     /locus_tag="ACG816_002140"
     CDS             complement(1287..2432)
                     /locus_tag="ACG816_002140"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4920997.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2445..3362)
                     /locus_tag="ACG816_002141"
     CDS             complement(2445..3362)
                     /locus_tag="ACG816_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4920998.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3790..4353
                     /gene="efp"
                     /locus_tag="ACG816_002142"
     CDS             3790..4353
                     /gene="efp"
                     /locus_tag="ACG816_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4920999.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4454..4624
                     /locus_tag="ACG816_002143"
     CDS             4454..4624
                     /locus_tag="ACG816_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921000.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4839..6098
                     /gene="lysA"
                     /locus_tag="ACG816_002144"
     CDS             4839..6098
                     /gene="lysA"
                     /locus_tag="ACG816_002144"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4921001.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6085..6885
                     /gene="dapF"
                     /locus_tag="ACG816_002145"
     CDS             6085..6885
                     /gene="dapF"
                     /locus_tag="ACG816_002145"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4921002.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6888..7769
                     /gene="dapA"
                     /locus_tag="ACG816_002146"
     CDS             6888..7769
                     /gene="dapA"
                     /locus_tag="ACG816_002146"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4921003.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7766..8482
                     /gene="dapB"
                     /locus_tag="ACG816_002147"
     CDS             7766..8482
                     /gene="dapB"
                     /locus_tag="ACG816_002147"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4921004.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8488..9183
                     /gene="dapD"
                     /locus_tag="ACG816_002148"
     CDS             8488..9183
                     /gene="dapD"
                     /locus_tag="ACG816_002148"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4921005.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9199..10200
                     /locus_tag="ACG816_002149"
     CDS             9199..10200
                     /locus_tag="ACG816_002149"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4921006.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10197..11558
                     /locus_tag="ACG816_002150"
     CDS             10197..11558
                     /locus_tag="ACG816_002150"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4921007.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11518..12669
                     /locus_tag="ACG816_002151"
     CDS             11518..12669
                     /locus_tag="ACG816_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4921008.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12644..13813
                     /locus_tag="ACG816_002152"
     CDS             12644..13813
                     /locus_tag="ACG816_002152"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4921009.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13988..>14214
                     /locus_tag="ACG816_002153"
     CDS             13988..>14214
                     /locus_tag="ACG816_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4921010.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNV"
BASE COUNT         4593 a         2508 c         3023 g         4090 t
ORIGIN      
        1 tagttcatct ttttttattt ttattaagtt aaagaacgga ttcaattgca gcggtcattt
       61 tcaatggaga agtttgtggt gcaaatcgtt cgattacttt gccatttcta tcaatcaaaa
      121 atttagtaaa attccatttt attgctttag tgacagtacc aggagcttca tttttaagat
      181 aagtaaaaag aggatctgta ttttctccat ttacgtcaat caattgatgc atagggaagg
      241 taacgccata agtggtttta caagcttctg ctgcttcagt cgctgaatct agctcttgtt
      301 taaattgatt ggaaggaaag cctaagataa ccagaccttg tgtctgataa gcttggtaaa
      361 gttcttctaa ttgtttaaat tgaggtgcta gaccgcattt tgttgcagtg ttaacaatga
      421 ttatcggttt gcctgcatat tttttcaatg aatagacagt gccgtcttct aaagtagttt
      481 caaaattata aatagacata aagttttctc ctttctattt tatataataa aaagagcgct
      541 aaactgaaaa ctagctattt gttttcccgt ttagaactct tttcagacaa taaatattta
      601 ataaaatacg aagataagat agctatattg cttctcatct ttacgttctt ttagaaacta
      661 tgcttctact tcagaccatt catcgcgttt tgctaaaaat tcacgagcca aagcttgtgc
      721 accttctagt gtatggctgg cagcccagcc acattgtgtt tcattgcttg caggtacttc
      781 agtagcttca agaacatctt tcattgttgc ttcaagaaca tctaaaatgt cttcgtagct
      841 ttcatgattc aatacagtca agtaaaagcc tgtttggcat cccattggag accagtctac
      901 gatgtaatct gcgtgattgc ggattagttc ggcagtcaag tgctcaagtg aatgtagact
      961 tggcatgtcc atgtgttctt tatttggttg tttaaagcgg acgtcgtatt taatgatgac
     1021 atcgccatgc gcacccgttt ttcgatcagc tacacgtaca taaggtgctt tgacttttgt
     1081 atggtcaaga ttaaaacttt cgacattcat tctcatttgt aagactccta tctgtttgtt
     1141 cattcgtttt gatgcttttc actaaagaat tatttagtga aatttacaaa aacgtctttc
     1201 tacttgattc taacacttat cgtttccaag gtgtagcaat ctgaaatatt ttttagattt
     1261 ttctgctaag tcgtttattg ggacttctag ttcttgattc tgtcaaatgc ctgagctaaa
     1321 tcgtttatga gatcctgacc atactcaatt cctacagaaa gacggatcaa gccgtccttt
     1381 atgccagcct cttctcgttt ttcttttgga atagacgcat gagtcatgac agaaggaatt
     1441 tccactaaac tttctacgcc acctaaactt tctgctaaag taaatagttg gagtgattca
     1501 acaaatggaa tagcagattc ttcatttttt aaagtaaaag aaatcattcc gctaaagccg
     1561 ctcatctgct gcttagcaac tccatggagt tcatgatcgg gtaatcctgg atagtagacc
     1621 ttttctactg ctggatgaga aaatagaaaa tcagctacca caaaagcatt tttttgatgt
     1681 tcctccatac gaacacttag tgtcttgatt ccgcgctgaa gtaaccagct atcttgggga
     1741 ccaagtacgg ctccgatagc attttggtaa aatccgatct gatcggctag atctttatgg
     1801 ttagaagtca ctaaaccagc tactacatcg ctgtgtccgc ctagatattt ggtaccgcta
     1861 tggacaacga tatcagaacc gagatctaaa ggtctttgaa aataaggagt agcaaatgta
     1921 ttgtcagcaa taactatcaa tccatgttga tgagccagag tggcactttt ttctaaatca
     1981 gtaatcttta gaagcggatt gctaggtgtt tcaagataaa gtgcttttgt attgggtttg
     2041 atggattgtt ctattttatc aagattactc gtatctatga tcgtgtactc gagaccattt
     2101 ttagtaagaa ctttgtcaaa tagacggaat gttcctccat agacatcatc tcctaataag
     2161 atatgatcgc cagcttgaaa aagagaaaaa acggcatgaa tcccagaaag accagaagaa
     2221 aaagcgaatc ctcgaacacc gccttccaaa tcagcaatca gttcttccaa tgcaaaacga
     2281 gtaggattac cagatcttga atattcatat tgtttaggct ggccgacgcc atcttgccga
     2341 taagtggatg tttgataaat aggtacactg acagctcctg ttgtgggatc ttcactgatg
     2401 cctccgtgga tcaattttgt ttgaatgtgc atgtttcttt cctcctataa gtaaatgttt
     2461 tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg accaggtggt
     2521 aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact tccaactaat
     2581 agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc agagattgtc
     2641 tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac accgataccc
     2701 tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg ttctacaccg
     2761 attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc aaaggttcca
     2821 ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg aaagatttct
     2881 ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg aagaggcaga
     2941 tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc gatgattcct
     3001 tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg ttgtttttct
     3061 aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag tgcggccaga
     3121 gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt ttcattgatt
     3181 tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt gatacttcct
     3241 ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat agaatagtgc
     3301 tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt cgagataatc
     3361 atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat cagctccttg
     3421 aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact cattctatga
     3481 agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac aacagcaacg
     3541 attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa tttttattaa
     3601 cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt tcgatagcat
     3661 cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga tagagaatac
     3721 tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag aaaagaagga
     3781 gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt acaagacggc
     3841 aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa tacagttatg
     3901 cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt cagaccagaa
     3961 gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta cacgatggat
     4021 gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc tgtagaaaca
     4081 gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca atttttcggt
     4141 agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt ggaaacacaa
     4201 ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat ggaaactggc
     4261 ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat caatacagca
     4321 gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat aagaatttga
     4381 aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag aggttttttg
     4441 tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt tgaaagatga
     4501 atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg aaatgattca
     4561 taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat tttttcatca
     4621 ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt cgggtatttt
     4681 gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat actagacaat
     4741 tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa atgaaattcc
     4801 aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg ataattggcg
     4861 gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg tatgatgaag
     4921 aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa cgttttgata
     4981 caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat ttagttcgtg
     5041 aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct ttgcaagctg
     5101 attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag gaactgatct
     5161 atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa gttgaattat
     5221 tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg ttgaatgtcg
     5281 gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca aaattcggca
     5341 tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa agcagctatc
     5401 tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt actgcttggt
     5461 tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct ctttccttga
     5521 atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc ccagtcgaag
     5581 aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga aaactgacca
     5641 ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt acaacactat
     5701 acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta gatggcggga
     5761 tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat ttagcagcaa
     5821 aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt gaatcaggcg
     5881 atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt ttagctgttt
     5941 attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg acacgaccag
     6001 cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa gcattttctg
     6061 atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg cggaaatgat
     6121 tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc ttctttatgc
     6181 acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc gttggaaatg
     6241 atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat tcgctgtttt
     6301 gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt agagacatta
     6361 gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag cgtagatatg
     6421 ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc aataaaaaac
     6481 caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt aggaacgatt
     6541 cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag aggtgaactg
     6601 atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga agtagtcaat
     6661 gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact tgcttgcggt
     6721 acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc agcagatcag
     6781 gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc gatacaaatg
     6841 gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg ataaaaggtt
     6901 cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat gctggtttgg
     6961 aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt ttaggtacaa
     7021 caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa ctgacagtaa
     7081 agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat acgaaagaaa
     7141 cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg gtcatcaccc
     7201 cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca attgcagaag
     7261 cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg tcgattccaa
     7321 tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa gaagcctcag
     7381 gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc ttcctttaca
     7441 gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt gtaataagtg
     7501 tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg caggacggaa
     7561 gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat gccttgttca
     7621 gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta ccttccggac
     7681 ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta gctcagcttt
     7741 tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg gacagatggg
     7801 aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt tacggacggc
     7861 acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta ttgatttttc
     7921 tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac ttccgttagt
     7981 cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac atgccaaaca
     8041 tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga atcaactaat
     8101 caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa aacatcatag
     8161 tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa tccaagaagt
     8221 gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa accaaattgg
     8281 tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt ttgctgctac
     8341 tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg ctgcaggcgc
     8401 agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat tagaagattt
     8461 attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg ctacattcaa
     8521 acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc aatcgaattt
     8581 aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga ctatgagacg
     8641 attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat cgaaactgct
     8701 gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg tatcgaacca
     8761 ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat gatgggagcg
     8821 attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg tgctgtctta
     8881 ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt cttagcaggt
     8941 gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact ggtaggtgcg
     9001 aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc agcaggagca
     9061 gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc acgtgtatta
     9121 aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt acgcgaatta
     9181 tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc aaccggatta
     9241 gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg acgattgaaa
     9301 ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa gaaacacact
     9361 gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt ttctgcaggt
     9421 gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt tgtcatagat
     9481 aattcctcag catggcggga agattcggat attccattaa tcgttccaga ggtcaatctg
     9541 gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat tcaagcggta
     9601 cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta ttcgacctat
     9661 caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac gcaaaatgac
     9721 gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga aatcgatcag
     9781 cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac ccgtaagatt
     9841 ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat cgaaaatgga
     9901 catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga aattagagag
     9961 tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca taaatatccg
    10021 acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag aaaagacact
    10081 tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa aggagccgct
    10141 gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt ggcaagatga
    10201 gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa gagcaattca
    10261 aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt gtttctgctt
    10321 taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg atttatgctt
    10381 accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat cgtttcattc
    10441 aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca cgcatacaga
    10501 aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt gaatatctga
    10561 ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa gacctgattt
    10621 tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc acaaaagcat
    10681 ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct gcaggaaaag
    10741 aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg aagatgttgc
    10801 aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct gatccgcgaa
    10861 tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga gagatggctt
    10921 atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag gctgatatcc
    10981 ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata tcgaatcacg
    11041 aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt ctttccatca
    11101 ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca atgagcattt
    11161 ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat atcggagtga
    11221 tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa ttaaaagaaa
    11281 cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct gtcgttgggg
    11341 gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg aacaaattag
    11401 aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc atcggtgtac
    11461 cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg aatacggatg
    11521 ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca tgaattcaag
    11581 acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca tgaaatcggc
    11641 gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc ttttcgaaca
    11701 gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc taagcatgaa
    11761 ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg gttgactgac
    11821 tttataacta aacacattga ggatcttatt tataatgtag tactgatcta tcagccatca
    11881 gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga taaatatgaa
    11941 gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga agttttctct
    12001 cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa aggccagtct
    12061 gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt acgatttttg
    12121 aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct aatgaaattt
    12181 ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat tgtatccgga
    12241 agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt agaccattta
    12301 gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg ctatccagct
    12361 gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact acatgaatta
    12421 gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata tccatgtgta
    12481 tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt cgattttgat
    12541 atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac agaaaatctt
    12601 cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga atttatccaa
    12661 tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat tcgatcagca
    12721 aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt ttccaactcc
    12781 tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc actataccgg
    12841 aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac agcgttatgg
    12901 attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg aagcgattgc
    12961 ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag cgcctgctta
    13021 ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga tagatacttc
    13081 tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac aatatggatc
    13141 agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga cattgtctgc
    13201 aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg ttataagtga
    13261 tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta cttatcttcc
    13321 agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg gttggcgagt
    13381 gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc atcaatatct
    13441 ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa taaatggggt
    13501 gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc tggtccagca
    13561 attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact tgttttgcaa
    13621 actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag cggaacaggc
    13681 aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt atatccggat
    13741 ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca aagacttttt
    13801 aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg cggtatattc
    13861 tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt ttgattcata
    13921 actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat tcaaggaggt
    13981 ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag ggcatcaatc
    14041 gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat ttgaagcagg
    14101 agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca aggtaacaga
    14161 tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg ttca
//
LOCUS       DAZUKT010000069        14181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000069 DAZUKT010000000
VERSION     DAZUKT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.070"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG816_002154"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG816_002154"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4921011.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACG816_002155"
     CDS             complement(2261..2512)
                     /locus_tag="ACG816_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4921012.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACG816_002156"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACG816_002156"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4921013.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACG816_002157"
     CDS             3462..3680
                     /locus_tag="ACG816_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921014.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ACG816_002158"
     CDS             complement(3969..4481)
                     /locus_tag="ACG816_002158"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4921015.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACG816_002159"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACG816_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4921016.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ACG816_002160"
     CDS             complement(6934..7377)
                     /locus_tag="ACG816_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4921017.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ACG816_002161"
     CDS             complement(7378..8166)
                     /locus_tag="ACG816_002161"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4921018.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACG816_002162"
     CDS             complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACG816_002162"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4921019.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ACG816_002163"
     CDS             complement(9281..9868)
                     /locus_tag="ACG816_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4921020.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACG816_002164"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACG816_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4921021.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ACG816_002165"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ACG816_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4921022.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ACG816_002166"
     CDS             complement(12785..13720)
                     /locus_tag="ACG816_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4921023.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13906..>14181
                     /locus_tag="ACG816_002167"
     CDS             13906..>14181
                     /locus_tag="ACG816_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921024.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT         3957 a         3158 c         2502 g         4564 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataaccc gcgaattgta aaattaagtt agaaaaataa aaaggcattt atggtacact
    13861 caaattgtat ttccgacgaa agaaaacaaa ggagtgtaac cataaatgac ctacaaacat
    13921 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
    13981 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
    14041 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
    14101 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
    14161 cttggttgga cgcctgacgt c
//
LOCUS       DAZUKT010000070        14001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000070 DAZUKT010000000
VERSION     DAZUKT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.072"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..2426
                     /gene="acm"
                     /locus_tag="ACG816_002168"
     CDS             261..2426
                     /gene="acm"
                     /locus_tag="ACG816_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4921025.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2698..3183)
                     /locus_tag="ACG816_002169"
     CDS             complement(2698..3183)
                     /locus_tag="ACG816_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4921026.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3273..3618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3663..4304
                     /locus_tag="ACG816_002170"
     CDS             3663..4304
                     /locus_tag="ACG816_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4921027.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4323..4958
                     /locus_tag="ACG816_002171"
     CDS             4323..4958
                     /locus_tag="ACG816_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4921028.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4976..5791
                     /locus_tag="ACG816_002172"
     CDS             4976..5791
                     /locus_tag="ACG816_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4921029.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6110..7546
                     /locus_tag="ACG816_002173"
     CDS             6110..7546
                     /locus_tag="ACG816_002173"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4921030.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7735..10182
                     /locus_tag="ACG816_002174"
     CDS             7735..10182
                     /locus_tag="ACG816_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4921031.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10412..11479
                     /gene="rlmN"
                     /locus_tag="ACG816_002175"
     CDS             10412..11479
                     /gene="rlmN"
                     /locus_tag="ACG816_002175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4921032.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11469..11675
                     /locus_tag="ACG816_002176"
     CDS             11469..11675
                     /locus_tag="ACG816_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921033.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11672..12775)
                     /locus_tag="ACG816_002177"
     CDS             complement(11672..12775)
                     /locus_tag="ACG816_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4921034.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13327..13875
                     /gene="aac(6')"
                     /locus_tag="ACG816_002178"
     CDS             13327..13875
                     /gene="aac(6')"
                     /locus_tag="ACG816_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4921035.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4817 a         2307 c         2957 g         3920 t
ORIGIN      
        1 gttaaaaatt ggaaatatag attgaaaaaa tgaaagcaat aagtaaaata aaatgaatat
       61 atgcgaaaaa ataatttaca ttaggaaaaa ccttgtaaaa acagtttttt ggcatattta
      121 ttttttataa atatttgtaa aataactatc aattttttag ttaaaagatt ggaaatgagt
      181 gggggaggaa ttgaagaaag gatttttgag agatgatata gtaggtttta tttttagtta
      241 aaatagggga gatagaaaaa atgaaaaagt gtagaaatat tattttatca atgttattta
      301 ttattactaa tataacatca ctgattcctg ttcatgtcta tgcggatgca ggcagagata
      361 tcagcagtaa tgtcacttcg ttgaccgtgg acccaacgaa tattacagat ggaggaaaca
      421 taaaagtaaa attctcgttt gacgaaaaaa aacaaaatat ccaaccaggt gattatcttt
      481 ggatcaattg gccaagtgaa ggaaatattc gcggtgaagg attccaaaaa gaaatcccat
      541 taatgattga aaataaaaat gttggaactt taacggtcag aaaagattct gcgcaggttg
      601 tgtttaatga aaatattaaa aacctagact cagtagaagg ttgggggcaa tttgagatac
      661 aggccagaaa cgtaaccgat accggtgaag aaaatacggg accattcacc gtgacgagcg
      721 gtgataaaac agctactgta aatgtgacaa agccagcttc tggttcttct tctagcgttt
      781 tttattataa aacaggagac atgctacctg aagatactaa gcacattcga tggtttttga
      841 atattaacaa taacggcact tatgttgaac aaccagttaa aatcagtgat gaaatccaaa
      901 gtggacaaag attagatcca agtacgtttg aaattaatca aatacactta ggtgaacaga
      961 aggtttatag aggagaagag ggaatccaac aatttttaca agatttcccc agcgctacat
     1021 ttaatttttc agtaactgat aattacattg aaataactat accaaaaaac tttgtcaatc
     1081 ttaggaaaat aatggtgagt tataagacga tcatagaaaa cccagaacaa ataaattttg
     1141 agaatcattc ggaagcttgg tttaaagaat ttaataaacc tgctgttgat ggagaaagtt
     1201 tcaatcatac agttaaaaat atttcagctt ctggtggagt caacggtaca gttagaggcg
     1261 aattgaagat tttcaaatat atcaatgaca cagagattgg tatcccaaat gtgactttcg
     1321 aattaagacg agcagatgaa cagcctattc aaggacagtc tagtatcctg ttgacttcga
     1381 atgaacaggg agaagctagt atcaaaggcc tgcaagtagg agactatgtt gtaaaagaaa
     1441 aagaagcacc caattggatt gattttgatc cactaagttc aaatgaactc aaattctcca
     1501 taaacgaaaa tgatacagag ggagtcagtt tacctattta taacaagaaa aaggttacga
     1561 acatcacagc aactaaaatt tggaacggtg gaaccactcc aagaccgagt atttatttta
     1621 aattattcag agcaagtaca aacaattggg aaccagttcc tgatgcagaa acaaaacgat
     1681 tagacaatgg aataacttca gtgacttggg aagatatcca acagtatgat gatagtggaa
     1741 acgaatatac gtttaaagtg caagaagtag atcaaaacgg aaatgattat gtcccttcag
     1801 gttatcaaaa aattgaaaac gggctagtcg ttacaaatga gaataaagaa gtcatttcag
     1861 tctcaggaca aaaaacatgg gaagacaatg agaaccaaga cggcaaacga ccgacgacga
     1921 tcacagtaaa tctattagcc gacggtcaac cgatccaaca caaagaagta agtgaaaaag
     1981 atgattggcg ctatcgtttc acaaatctgc caaaatacag agacgggcaa gaaatcatct
     2041 atactgtaac ggaagacaac gtgcccgaat acagtacgac gattgacggg tataacatca
     2101 aaaacagtta tacaccggaa aaaacaaata tatctatacc aggtagccat ataacaccgt
     2161 ggaaaaattt tccagagaag acagagagag agaaaattag aaatctattc tctcaaaaat
     2221 tgcaaatatt tgctcataca ggagaatcca gcgaacaaag aagcaattat attagtaaga
     2281 gagctgtacc taaacagata acaaataaat caaaaagcat tcttcctaaa acaagtagta
     2341 aaaaatctag ttttgctgta atcattggtt tattgatagt aactatctgt gctgggatat
     2401 tcttacaaaa atataaaaaa gtttagttag aatataatat tttttgttaa atatgactga
     2461 aatcaaaaga ataaaattcc gatttataat cgaaaaaaga agcattttta tatatgcttc
     2521 ttttttcgta gaatcaataa atatggattt ggctaactat ggaagaatac tcgtattagt
     2581 tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt ctgatatatt
     2641 ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac tctatcatta
     2701 caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa
     2761 atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat cttgacgatc
     2821 aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca atgctaatgg
     2881 agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga
     2941 taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct tttcatcagg
     3001 caagatgaca agatagattt gttttccttt tcttgatttc aataatagat tttttacttg
     3061 cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat gatcgacttt
     3121 ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg ttgcacgact
     3181 catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg acaaatgaaa
     3241 aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga agagtagccg
     3301 ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac cgtgaaacac
     3361 atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc gttacagctg
     3421 cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg taaaaaaagg
     3481 tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg ttttcatctg
     3541 aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag gtggaaccac
     3601 gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag gaggaaaaga
     3661 agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc acaacattga
     3721 aattattttt atttaccttg attggctcat taccattagg gattgtttta gcattaggat
     3781 tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg gttatgcggg
     3841 gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg attgggatcg
     3901 tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca gcttattttg
     3961 cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag
     4021 ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa gtgacaaaga
     4081 ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct tccttgatct
     4141 atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga
     4201 ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt attctaacaa
     4261 tcgtaatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata ggaggaagaa
     4321 atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata atcaaagatt
     4381 tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc ggtggcggta
     4441 aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga gaattttatt
     4501 tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg
     4561 tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt accttagcac
     4621 caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa gaattagcgg
     4681 cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct ggcggacaaa
     4741 aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg gcttatgatg
     4801 aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg atcctatcat
     4861 tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt gcagaaaatg
     4921 tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga tagagatgaa
     4981 aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa
     5041 caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg
     5101 tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc tcacaggatt
     5161 tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag actttcagcc
     5221 cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt tgatatggaa
     5281 cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac cttatatgga
     5341 aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg gacaaatgaa
     5401 taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta cgaaacaacc
     5461 agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata gttttaatga
     5521 agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata aagtatacgc
     5581 agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa aaggttctta
     5641 tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag tgaaaaaaat
     5701 caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat ccgataaatg
     5761 gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca aaagacgtgt
     5821 cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg
     5881 aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt cggtattaag
     5941 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac tactcgaaac
     6001 taagcgactt tttcacaacc tcctttacat aattttattt tgttgtggaa ggggtttcgt
     6061 gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa tggaacacaa
     6121 agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag cgtatcaagt
     6181 agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat ttgtacggat
     6241 acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact atcatcgtta
     6301 taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc
     6361 ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc tcgcttttta
     6421 tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga ctttatatca
     6481 ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac gtcagatcat
     6541 tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga aagtccatta
     6601 ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt tagcagcaca
     6661 tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg ctaatttagc
     6721 aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa tcggaccaag
     6781 ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc tggctgctga
     6841 aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg gaaaatatcc
     6901 gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg aagctggcga
     6961 tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact atcaaacggc
     7021 gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata cgacaggaaa
     7081 aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg agaatccatt
     7141 tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga tcggactgcg
     7201 tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct taggagagta
     7261 tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt acttacaaag
     7321 ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag gctattgcac
     7381 ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat atggatttgt
     7441 atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga aagacagtta
     7501 ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca catacttaaa
     7561 acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt tagaaattaa
     7621 gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca aacaaaactt
     7681 tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata ataaatggca
     7741 caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag
     7801 cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt tgctgtcttc
     7861 ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg gatcattgca
     7921 ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg gattgtcatt
     7981 aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat actcttttat
     8041 ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac tggggaacca
     8101 aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag tcaagtagac
     8161 aataacgttg gtggtgggat gattggagca atcctttatc attttactta cttcttgatt
     8221 gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc attcctgttc
     8281 agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga acgagtacag
     8341 gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga
     8401 atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa
     8461 aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct
     8521 gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt tccaatcgac
     8581 agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa
     8641 cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg tacctcatta
     8701 gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac agttgatttg
     8761 cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga aaaaaatatc
     8821 ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc
     8881 ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa agtaagtaaa
     8941 attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa
     9001 gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa gatcagtatg
     9061 gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt actagaagta
     9121 cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa gatgcctcac
     9181 ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg gattatcaca
     9241 agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga tccgaaaatg
     9301 gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt gacgaaccct
     9361 agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa
     9421 ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca acagaaaaac
     9481 ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt agatgaatta
     9541 gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg tttagcgcaa
     9601 atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag tgtggatgtt
     9661 atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt atcaagcggt
     9721 acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg gcgaggagat
     9781 atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc attcatttct
     9841 gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc tcattatgaa
     9901 gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca gccacaagat
     9961 gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag tatttccctt
    10021 ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga tgaactggaa
    10081 gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt cattgagcag
    10141 gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg tgatgaaggg
    10201 ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt
    10261 tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa
    10321 gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta
    10381 gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac
    10441 gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca
    10501 agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc
    10561 gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt
    10621 cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat
    10681 gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt
    10741 aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct
    10801 gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa
    10861 tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta
    10921 cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc
    10981 tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa
    11041 cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc
    11101 gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta
    11161 tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga
    11221 tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata
    11281 tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa
    11341 agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg
    11401 taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa
    11461 aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag
    11521 ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta
    11581 taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga
    11641 cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc
    11701 tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg
    11761 tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt
    11821 ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt
    11881 tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca
    11941 gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg
    12001 taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa
    12061 ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat
    12121 cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt
    12181 ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag
    12241 tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg
    12301 cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga
    12361 gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt
    12421 tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta
    12481 cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt
    12541 catgctggat caactgttca atatttccat aagttgaatt acccccgtac cagagactgg
    12601 cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg
    12661 cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt
    12721 tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca
    12781 tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg
    12841 ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac
    12901 accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac
    12961 aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta
    13021 tctaaaaatt ctaaaaatta agattattat atgaaaacag gttttatttg tcaattcata
    13081 attggttttt tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc
    13141 tccaattgat ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt
    13201 tattttataa gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt
    13261 ttgagcgaga acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga
    13321 agacgtatga taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct
    13381 gatttactga gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa
    13441 gaaatgatga atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga
    13501 tttattggtg caatccctca atacggtatc acaggttggg aattgcatcc attagttgta
    13561 gaaagctccc gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa
    13621 gtagcttcca gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca
    13681 acgttaagtc aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac
    13741 cttcgtgaac atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta
    13801 ccaaatgcaa atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga
    13861 ccaaattctc aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa
    13921 ggaatttacg aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc
    13981 tacttttgga acaccgtgta c
//
LOCUS       DAZUKT010000071        13873 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000071 DAZUKT010000000
VERSION     DAZUKT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.073"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..1233)
                     /locus_tag="ACG816_002179"
     CDS             complement(49..1233)
                     /locus_tag="ACG816_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4921036.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1258..2265)
                     /locus_tag="ACG816_002180"
     CDS             complement(1258..2265)
                     /locus_tag="ACG816_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4921037.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2437..2796)
                     /gene="comGG"
                     /locus_tag="ACG816_002181"
     CDS             complement(2437..2796)
                     /gene="comGG"
                     /locus_tag="ACG816_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4921038.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2768..3193)
                     /gene="comGF"
                     /locus_tag="ACG816_002182"
     CDS             complement(2768..3193)
                     /gene="comGF"
                     /locus_tag="ACG816_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4921039.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3156..3503)
                     /locus_tag="ACG816_002183"
     CDS             complement(3156..3503)
                     /locus_tag="ACG816_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921040.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3475..3945)
                     /gene="comGD"
                     /locus_tag="ACG816_002184"
     CDS             complement(3475..3945)
                     /gene="comGD"
                     /locus_tag="ACG816_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4921041.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3920..4198)
                     /gene="comGC"
                     /locus_tag="ACG816_002185"
     CDS             complement(3920..4198)
                     /gene="comGC"
                     /locus_tag="ACG816_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4921042.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4195..5244)
                     /gene="comGB"
                     /locus_tag="ACG816_002186"
     CDS             complement(4195..5244)
                     /gene="comGB"
                     /locus_tag="ACG816_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4921043.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5201..6130)
                     /gene="comGA"
                     /locus_tag="ACG816_002187"
     CDS             complement(5201..6130)
                     /gene="comGA"
                     /locus_tag="ACG816_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4921044.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6262..8016
                     /gene="ade"
                     /locus_tag="ACG816_002188"
     CDS             6262..8016
                     /gene="ade"
                     /locus_tag="ACG816_002188"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4921045.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8114..8776)
                     /gene="trmB"
                     /locus_tag="ACG816_002189"
     CDS             complement(8114..8776)
                     /gene="trmB"
                     /locus_tag="ACG816_002189"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4921046.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8846..9628)
                     /locus_tag="ACG816_002190"
     CDS             complement(8846..9628)
                     /locus_tag="ACG816_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4921047.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9832..11043)
                     /locus_tag="ACG816_002191"
     CDS             complement(9832..11043)
                     /locus_tag="ACG816_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921048.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11036..11773)
                     /locus_tag="ACG816_002192"
     CDS             complement(11036..11773)
                     /locus_tag="ACG816_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921049.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11911..12345
                     /locus_tag="ACG816_002193"
     CDS             11911..12345
                     /locus_tag="ACG816_002193"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4921050.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12338..12682
                     /locus_tag="ACG816_002194"
     CDS             12338..12682
                     /locus_tag="ACG816_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4921051.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12862..13872
                     /locus_tag="ACG816_002195"
     CDS             12862..13872
                     /locus_tag="ACG816_002195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4921052.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4395 a         2844 c         2341 g         4293 t
ORIGIN      
        1 attccctctt gcatgcgttt catcttttat cctaacgaat gctttggctt agccgcgcag
       61 acgctccaca tcacgggcaa tcattagttc ctcatctgtt gggatcacta atactcttac
      121 ttttgattct tttgtagaga tatctacttc ttcaccacgc aattcattca acgctggatc
      181 gatttcacaa ccaaaccaag acataccttt gatgatttca ctacgtacat gtccgtcatt
      241 ctcgccgata ccagcagtaa agacaattgc atcaacacca ttcatcgttg tcacataacc
      301 gccgatgtat tttcggatgc ggtccacgaa aatgtcataa gctaaacgaa tatgttcttt
      361 ttcatagttc ttttcaagat cacgcatatc actagacaat cctgttaggc ctagcagacc
      421 agatttcttg ttcaaaacat tgatcatttc atcaatatca atctctaatt tttccattaa
      481 atatgggaga acagctggat cgatatcacc tgaacgtgta cccattgtca ccccagctaa
      541 tggtgtgaat cccatagaag tatccatgga tttacctcca tcgactgctg tgatcgatac
      601 accatttcct aagtggcaag tgatgatttt tagttcttcg atcggtttgt tcaataattt
      661 tgccgcacgt tccgacacat aacgatgact tgtgccatgg aagccatatt ttcttacttt
      721 gaaatcttca taataagaca ccggtaagct atataaataa ttttgctttg gcattgttga
      781 gtggaaagat gtatcgaata cagccacact caaaatgtca ggtaaaatct ttttaaacgc
      841 tttgatcccc attaagtttg caggattatg caaaggagca aattctgcta attcagcaat
      901 gcggtccatt actttctcat cgatcactac tgaatcccca taagtttctc ctccgtgaac
      961 aacacggtgc cctactccgg tgatttcatc ataagagctt aaaatttgca gttcaatcaa
     1021 ctgatctaac agcattttta ctgctacttc gtggttttcg atatccatga tttgttcgaa
     1081 tttttgatta tcgccgtact tgatggtaaa aatagaatct tgtaaaccga tacgttcgac
     1141 gatccctttc gcaactacgg tttcttctgg catttgatat aattgccatt tcaaacttga
     1201 actcccagca ttgattgcaa ttgttttaga catatgactc tccttctcgt tgaatgatta
     1261 cagagcagat gatttccagt ttttaaactc gttgaaaaat tccgtcacct tctctggttc
     1321 ttttaatgaa gcaagtttca caagcaagac ttcttttact tgttgtgctt gtggtccttt
     1381 tttctgtaag atcaaaatac ttttttgcga ttgtttatta cggaacaatt catctggcaa
     1441 ttgaatcatt ccttgaagat aaccttcttc tttaaaccat tttttgattt cttcactttg
     1501 atccgtttct aaaaatccac tcggcatcaa aaacaaacca aaaccatccg gcttcacata
     1561 tttcattgat tgctctagca ataaatgatg tgcataacta tggccttctt cggtacttgt
     1621 taaaaactct tttgcttttt cgtcattcgg ataataaccg attggcaaat cgctgattgc
     1681 aaaatcgaca ggatctatta ataattcctg caacccgtct tgatgaaagt attgaacatt
     1741 tgcttgtgtc aaatcacttg tagaagctgc gacagccaac aatgtatcat caatatccac
     1801 accaattcct tctgtttggt accccgcatt cgataggttt aaaagaactg ttaataacaa
     1861 gttgcccatt cctgccgcaa tatccagaat cttaaccggt gcttttttat ttgtgtatag
     1921 ttgttcaata agaaaaacaa ataaaaaacc gatactatca ggagtgagtt gatggttcgc
     1981 ctgcaaatgt tctgtctggc tgcccttcaa taaaagtaat tgagacagtc tgcgccattc
     2041 ttctggctcg aaagaaagtt tcttcaactc ttcataaaga gctgtgatcc gttgcgttgt
     2101 ctcttttgtc ggcactccat ccaccacacg tacttgataa tcatctatta aattttctgc
     2161 attttcgaca taggcttcta aaaaggaagt tcccaatgca ttctgtaaaa gctgaatagc
     2221 ttccaggttt tgattaaacc cttgttccat tttttctggt aacatggtgc acctcatttc
     2281 tagatatatt ataacaattt tttcacctgt ttaattctat cgaacaattg acaaaatttc
     2341 aatcgttttc actgtttttt atttgtgaaa cggcaaacat ttttttatta aaaacagtgt
     2401 tataacagaa aagagggcgt tttcacgaac attccatcat tctgtactag tatctgtatc
     2461 attttttact tcttctgtga accgaaagat aaacggtgag acttccacct ctaagaatag
     2521 ggactgttgt acccactgat atgttacaga tccagttgtg tagtaaaaaa ctccttccga
     2581 actttcggca agcgtagact gttccgataa agccatctgc ttcattattt ttgctatata
     2641 ataatttttg attcgtatat taagatccat tgacaaacgg tagctttcta atgctgccat
     2701 caataaaaac gtacataaaa gaaataaaag caagacagaa aataaaatag agcccttata
     2761 acattttcta ctgatcgttt tttgtccata tggcatatcc atcttctcca ttttcaaagt
     2821 gtacataaaa agaaattgtg tgttcttttt ccaataattg aagctcagtg atacttgtta
     2881 acataggttg atgtccgccg gaagttcttc ttttacgtat cattgcctga tatctttcga
     2941 tgatatactg tttatcctcg atcgtgtagt gcagttgctg atttttcaca gagacattgt
     3001 gactgctctt gagttcttct tctaattgtg ctaaaaatat tagccactct ttttgatctt
     3061 tacgctgcaa aaattcactt acttggatgg tttgctgaat caaaagattg aatgtgagca
     3121 atagactgct caatatcatc agtccaatta gacattcaat cagcgtaaat gctggaatta
     3181 cttgtttttt caatactgat cgcttctttc tccatattga caaacacttt ttcgattact
     3241 ccattattat cgacaatttg gatatcgtat gggatcattt cttcccttcg ctctggtctt
     3301 ttgtgataca tccgctgcgt cttgatatct tcataaatca ttcgaataaa tagcagttcc
     3361 tccccacttt tcttttctgc ctgaagcaaa aacagctggc caaagctcac taagtagaca
     3421 cagacagaag cgatcataag cgctgtaagg gactccaaca aaacaaatcc tgttttattc
     3481 aattcttttt gtatagcgac cactccctat ctggaattga tacgtaatcg tccgctcttt
     3541 ttcttcccaa taaaattgat aagcttttag tgaactttca ttcccggttc tagcagcaaa
     3601 agtaatagaa gcatggcgtt tcaaggtaat ctcatcagga atatctaata cttcccaatt
     3661 tattttatct ggattcggaa catgaaaaat gatttgattc tcttcactat tccaataaaa
     3721 gcctgtttgg atctgattca caatagcgat tttttgcgta gaatagattc gtttttcaaa
     3781 ttggccgaaa aattgataga ctgccaattc attcttccat gcagaaaatg cgagaactgg
     3841 aaaagaaagg atcgttgtaa gtaataaaat cagtaagagt gattcaaaca acgtatatcc
     3901 tgatgctaaa accttacctt caaggccgct gatagatttc tgcatgttct cgtttgatat
     3961 agccttcatt caacaattcc tccacagtag gaactcttcc attattttga agcgcataga
     4021 gctctttttg tgtgtctacc aactggatga tcgcttcacg actttcttgg tctacatgat
     4081 tcctataacg cgacaaatta gggacgaaca acaacactaa tacactgatg atcagtaaga
     4141 cgattagcat ttcgatcaat gtaaaaccgc tataataaga tatttttctc tttttcatgg
     4201 caaaacctcc tctatgcttc cgtaaacagg taataaaaca gcggcataaa ctaataaaat
     4261 caaagccgct acgcccaaaa aaatgattgg ctgaataaaa tgcatccagt gatgaagacg
     4321 tttgaaaagc tgttttcttg tccattcgct gtaaaaaatc agttctttac ctaaacttcc
     4381 ctttgcctct ccctgtaaaa caattttact gaattcttct gatagaaaac gataattctg
     4441 gaactgctca gccaatgaca tacctgcctc caatccttga atcaacttag aggcaagatg
     4501 ctgaatcaga ctttcttgtt tcgtttcctt taagcagact atgatttgcc ttaattcgaa
     4561 accttcatga aaaagctttc caatctctag tgacaaataa gccgattgat acaaggaata
     4621 tatcggtcca atcaataatt gtctgcttat ccattcactc tgatcaacag gagacaactt
     4681 catcagacga taccttacta gtaaagccaa tagagtaacc gctaggatga atagtagaac
     4741 ataccaatga aagacttgta tcagctgaat accccaatgc tcttgcatga ccattcctga
     4801 accaataagc tgaggcaaaa cgaacaaccg catactaaac aagataccta gcaaaaaggt
     4861 gagcaatagc atcggatatg caagcagctt tttcaaatca ttgcgaaatc cttcaacaag
     4921 ccgaaactgt ttggcaattt ccaacaaagt tgtcggcaga ttcccatgac gttcagctaa
     4981 ctcgatctgt accagttgct ctgctccata tcctatttgc gaaagtacct cactaaacgc
     5041 ttgcccttcc tttagctgat gttggatatt tgccaaaata acaggtggaa acgaacggat
     5101 cgtgagtaat aaataaaggg attcttgcag actaaaacca gataaaagca gttctcccat
     5161 ggtttgtgca aactggtttt gctggcttct tgttaatcct ttagaatagt tctgactgcc
     5221 aaatattttc ctgagcttct tcataacttt cctcccatga tttttttact gattcttcct
     5281 ccataaattg ataactccat aaaacagaag gatggtgatt ggcatcttcc aaaagttctt
     5341 gataaatgac accagctaaa cattcagata gctcttcttt tttctttgtc agttcatgga
     5401 ttctgaccaa tgtaccttca agattgcgtg catgtacggt tgcaaaaacc cggtgccctg
     5461 ttagtgctgc tcttaccgca gcatgtgcag ttgattcatc tcttatctcg ccaatgatta
     5521 gcaagtctgg acgatgccgt aatgcgagtc tcaaaagtgt atcataagtc tgtgctattt
     5581 tcggattgat ctgtagctgc agaaaaaacg gttcttctat ctctacagga tcttcgatag
     5641 taatcacttg caatttttct tccaaagcca accgatacat tagagaagtc ttgcctgatc
     5701 cgactggtcc gctgaaaagg tataatccac gtctgacggt tttctgtaaa atagttgcaa
     5761 aatctcgaga actaaagcaa cgatggggtg tttcaaaagt accgtataat agccgaatca
     5821 ctaaactctc tcgttgaagg taatctccta cagaagacaa ccgcagacga acttcctgtt
     5881 tttctaaaga ataagtaaat gcacctaact gagcttttct tttttctccg atatccatct
     5941 gtcccaaata cttaaatctt gagatcagtt gctgtccatc tgccgaagag actttcttcc
     6001 agaatgttcg tttttttcca tagcgaaaat agatgtaaaa tacttctgaa gctggcaata
     6061 tatatacgtc ttgggcatgt ttggcaaaac tttcacttat catttgatcc gataatcgtt
     6121 ttatttccat tattctcccc tccacaaaaa acatacgtct ttttgatctt tttgtttcaa
     6181 ataaaatgaa tttacaaaaa aatattggca aaaaccgaat ttatgttata cttataaaaa
     6241 tttatcttgg gaggcactcc attggaaaca acattaagga aatatatcga agtagcaagt
     6301 aaaaaaaagc ctgctgacct agtgatcaag catgctaaga tcgtcaacgt ctttacgaaa
     6361 gaaatcatgg aaaaagatgt agcaatctgc gaaggaatga tcgtaggaat cggtgaatac
     6421 gaaggaaaaa gcgtacataa tgctaatggg caatatctag tgccaggatt catcgatggc
     6481 catgtgcata tcgaaagctc acttttatca ccaagcgaat tttcaaaagt atccttgctt
     6541 catggagtta ctactgtagt gacagatccc catgaaatag gaaacattgc tgggagtaca
     6601 ggtcttgagt ttatgataga agatgcaaga caaacactca tgaatatctt ttttatgcta
     6661 ccttcttgtg tacctgtaac cccttttgag accaatggtg caaatttaaa tgcaaaaaaa
     6721 ttagcccctt ttttacaaaa acccgaagtt ttaggcttag ctgaagtcat gaactatcaa
     6781 gcagtagcaa ataatgaaac agacatccta gaaaagatcc aattgatgca ccagcataag
     6841 aaaaaaattg acgggcatgc agctggtatc ggcatggaag agttaaatgt ttatcctgca
     6901 gcaggtattc ggacggatca tgaagctaca actgcaaaag aagctaaaga acgtttggat
     6961 cttggtatgt atttaatggt tcgagaaggg accgttgcaa aagatctggc atcgctacta
     7021 ccggcgatca cacctgaaaa ttcaagaaga tgcttctttg tcaccgatga taaactgatc
     7081 aatgatttag taaaagaagg atcaatcgat catatcgtcc gtcaagctat tgcacttgga
     7141 atcgatccat tacaagccat tcagatggca acattgaatg cagcagaatg ttttgatttg
     7201 aaaacactag gtgcgattgc ccctgggtat caagctgatt tcttcttaac agacgatctt
     7261 accaagctgc caattcatga agttttctat aagggaacat tagttgttca atctgggcaa
     7321 ctccaagacc atttatttgc tgatcattca aattcatata cgtgttgtct gccaaaacta
     7381 aacgctcaac cattaaatcc acattcgtta gagctgcttc tttcttcttc acaagcccat
     7441 gtgatcgaaa ttatcccgaa tagtctagtg actaaagatt tggtagaaaa agtggaccga
     7501 caaggcgaac gattcattcc ttcaatcgaa aaagaccaat taaagattgc ggtgatcgag
     7561 cgtcaccatc aaacgggaaa cattggatta ggcatagtga aaggatttca gctgaaacaa
     7621 ggagcgatag cgacaacgat tgcccatgac tcgcataatc tagttgtcgt tgggacaaac
     7681 gacgaggata tgctgtatgc tgctaatctt ttgattcaaa aaggtggcgg gatgaccgtt
     7741 gtcaacaatc agcaggaaat agcttgtgtt tctcttccta taggcggaat catgacacag
     7801 gaaccttttt cgatcgtcta tgaacagttg aatgaattaa taactaaagc acatcaacta
     7861 ggagcagcta aaacctttga tccttttttg acactttctt tcttaacatt atctgtcata
     7921 cccgaattga agatcaccga caaaggatta ttttcatttt ctcgcttcca attgattcca
     7981 ccttgtggaa aaaccgatga aataaataca ttttaaatca aataaatccc agaaaataca
     8041 ttacatctgt aatgttcatt ttctgggatt tatttatgga tattaatgtt tttatgaaag
     8101 cagaaagtta gtttcacaga cttttctttt tggtcttatc ttgaaattga gcttctactc
     8161 gataaatacg ttgacctttt gaagaaaatt tttcttcata ctccgtcata atattgcctt
     8221 caaattgact ttgatgtaaa tctaaccata cctgtttgag gatcatgcca tattgagaaa
     8281 aacttgccag agaatactca aacaatcctt gattatctgt tttgaaatgg atttctccat
     8341 ttggacgaag aatctgttca tctacagcaa gaaaagtctt atatgtcaat cgccgttttt
     8401 catgacgttt ttttggccat ggatcggaaa aattcagata gatttgatct acttcgctgt
     8461 cagcgaaata ctctgtcaac gctgatccat ccacatgtag aagtttcagg ttaggaagcg
     8521 gttgttcgat caacttgtct agtgcaatcg aaacaacact gacctgcatt tctactccaa
     8581 tataattgat ttcaggatgg gcttttgcca ttccagtgat gaactggcct ttccccatac
     8641 caatctcaat gtggattgga tgatcgtttt caaacaattc attccatttt cccttccaca
     8701 atgttggatc actaattaca aaattcgggt gagccgctaa catttccgct gctcccggac
     8761 gatttcggac acgcattatt tatctccatt cctttcctct aattaaaaag gtctggccaa
     8821 atctccgcag agtttgggcc aaacctcagc cactgaaaat tcgttttaat tgtaaaattt
     8881 cttcgttcat ccgtcgatga tcttcacgtt tgcaatatct ggtgatttct tggagcaaat
     8941 tcatggccgc ataccaatga aatttttcaa gcatttcgtc agtcgggcgt atgccataac
     9001 tgatcagcca ctgattccag ctagacagtg gcacataatt tcctaaaatc gttgcaatat
     9061 ccaccgcagg gtctgcaaac atgattgaat cccaatcgac tagatataaa taatttttac
     9121 atactaacca attacgatgg tttacatctc cgtgtactac tgataagttt tccttatcta
     9181 ttgacgggat ctgctcacga agaaaacgat ggacaatttg cagaaattga tttttctgga
     9241 gttcttccgg taattctttt tcatattttt gcagcatctt ctccggcgtc atgacacgac
     9301 caccgatacg ggtcagcatg gttttaagag aatttgaatg atgtaaatgg tacaaaacat
     9361 caatcacgtc gtttctttga ccaatctcat taggagtcag caagcgacct tccagccact
     9421 cttgtgctgt caaagtatcg ccattccccg ttcgttttgt ccaaacaagt tttggtgtaa
     9481 ttccttccct tgaaagagca gccaacatcg gagtcgtgtt tcgtttaata aagactttgt
     9541 cctcatcacg catgcctatg tatgtttttc ctgtgtcgcc tttgatggga cgtaaccgcc
     9601 actcttggtc taactgaaac tccatcatgc attctcccgt cctcttactc tttgttttgc
     9661 agtacttatt attctagtcg cctgaacact acacgtcaag cacatatacg ttctttttca
     9721 tttaattgcc attcaaaaaa ccagcttttc tcaaaaaaaa tagaacgaac agcattctta
     9781 ctgtcaacgc tctatttttc attctcattt ttaccctaca cagctttgtt ttcagtctga
     9841 catcttcttc aaacgagttg gtacatatag tttaacaaag agcatagtaa caatcaaata
     9901 agccgctagc ggaacccatc gctgctgccc atctaatacg actgcagcaa tcaaaccaaa
     9961 aataaccgac gcgataatta atacccatgt gagtagatac tgaatcgcct gcttcttctg
    10021 attcttatgg attggataaa gctgaacgag aaccatatag cggaactggt tcgctaaagg
    10081 taacagttgg aaaccgatca aatataaaaa taagaaaccg ataatcagac taaaataaaa
    10141 gtcgactact ccggcaatca ataacccacc tataattact aatcggaaat aaagaccact
    10201 aaaatcagaa cctcttgcca atcttcttgc aaacaagtaa agataagtgt tttttgattc
    10261 tcttttaata tgagtcaata taggatccaa atagcgtctt cttttgatct ttgccgtaat
    10321 ttcaggaaca tctgtgaata aattgataaa ttgatagatc tgatgcaacc tatgttcttc
    10381 tacatgcacc attttctccc aatccaatcc tttttcttgt tgcttccaca aaaaccagta
    10441 atatagactt gcctgtacta atgcacctgc tattcctgcc catattagcc aataaaatga
    10501 tagaaacaaa aagatcagag aagaaaaaaa ccagcctaat ttccaataac gaataacttg
    10561 ttgatctagt cgaaacaatt ctgctctctt gatttgcaga tgactgaatt tcaaactcca
    10621 taatatgatc agatagacga aaaacatcga aaaggattgc tccgtactaa ccaccactaa
    10681 tggcatggcc aatccgcatg ccaatatcaa aaagatcaac ggaaaaatgc aagaatagcg
    10741 aaaagctcgt tccaaatagg tttgcatttc tttttcttta ggaagtaaaa aaacaatatc
    10801 tgctgcctct gcaaaagtag ccatcttacc cacgaataaa cagagcatcc aaaatacaga
    10861 gattatgagc cctccaattg caaacggtgg ctgcagggtt ttcaaccaat tagaataata
    10921 taatcccaca ccgccaatca agaaaagaca aactaaaaca aaatgatcgt tcaatacata
    10981 acgcatatat ttcatcattt tcttttgatg tcttgctaaa cgtttttgaa aaaaactact
    11041 catgtgcctg ctcctttgtt aaagcaatat agatttcgtc caaagaagaa tccggcagct
    11101 gaaattcttt tctcaacatc tcaagagaac cgtctgctcg tatctctccg ttatgtaaga
    11161 ccacaaaacg atcacaatat ttttctgcag tagctaaaat atgcgtagac atcaaaatac
    11221 cggctccttc ttttttcatt tctaccatca attcaagcaa tgcattgata gctagtggat
    11281 ccaagcctaa aaatggttcg tcgatgatat ataaagaagg ctcgatcaaa aaggcgcata
    11341 agatcatcac tttctgtttc attccctttg aaaaattagc tgggaaccag tcaagacgat
    11401 tagataaccg aaaagtctcc aacaactttt ctgctctttt cattgctata tccataggta
    11461 tatcgtaggc catcgcagtg acttcgatat gttctctcaa tgtcaattct tcatataaag
    11521 aaggagtctc tggaatatac ccaatttgtt tgcgatattc ttccgtattt ttctttaagg
    11581 atagatgatt gatttctatc gttccttttg aagcttccag cagaccgata atgtgcttga
    11641 tggtcgtact tttccctgct ccgttcagtc caatcaaccc tactatctct ccaggctgta
    11701 catcgaaact gatatctttt aacacaggga gatgtccgta cccccctgtg acatgatcga
    11761 taattaaact cattcttttt cctccgccat ttatttttat ttattatacc acgaagaaaa
    11821 tgacggtatt ttgttttttt ccttacaata gctttaactt atggtaaagt taagctgaag
    11881 tatttattct ttatagaaag gatgttttat atggaaaact gtattttttg caaaatcatc
    11941 aatcaagaaa tcccaagcta taaaatatat gaagacgaca aggtctatgc ttttttagac
    12001 atctctcaag caacgaaagg acacacatta gttgtgccta aacgtcatgt agcagatatt
    12061 tttgaatatg atcccgaact tgcaggagag gttttttcgc gtgtgcctaa gattgctcaa
    12121 gcattggaaa aagcttttcc agaaatgaaa ggtcttaata tcttgaataa caaccgtgaa
    12181 ctagcctatc aatctgtttt tcattcacat atccatttag tccctcgcta cagcaaagag
    12241 gatgattttt ctattcactt tgtcaatcat caagatagtt atggatcgga agaactaaaa
    12301 gctattcaag aaacaatcgt aaagcaggtg tcctgcgatg attaaaaact ttttcaaagg
    12361 actgttattt ggagctgctg ctggcactgt cggaggtctg ttattggcgc ccaggagcgg
    12421 gaatgaaaca agaaagaaac tgatcgaaga attggaagaa gcaacaactc tgacaaatga
    12481 tttaaataac agcctgaatc attttaaaag cgcattgatc gaaacaaaag aaacagctga
    12541 atctgtgatt cctgctttcc aagaagcaat caaaaaagat atcgaagatt ttaaattcca
    12601 agccgaacca cgtatcacac aaatcaatga acaagtagaa aaactaaaag ctgatctgcc
    12661 agagtatcct gaacaaatat aataatgatt attttctcaa cagatttaac tatataagtg
    12721 agaaaagttt gcgtatttcc taggaatgtg atgaaatttt gaattttgtt tgaaactacc
    12781 acgacaacgt ttttaaagga aatgggatat ggtatagtta atatataaaa ataataacta
    12841 attacaggag tgcaaatttt aatgaaaaaa aaatcaatta ttttggcagc tactagtgcg
    12901 ttggctgttt taactctagc agcttgttca ggcgatacta ataaagatat cgcaacgatg
    12961 aaaggcggaa cgatcactgt atctgatttc tatgatgaag caaaacttga atcatcgaac
    13021 caatccttag tacaacggat gatcatttat aaagtattca ataacaaata tggcgataaa
    13081 gtaaccgata agcaagtgga tgcagaatac gacaaacaag cgaaatcatt aggtgatact
    13141 tttgaatcac aattagaagc tgctggatat acgaaagata cttataaaga atatattcgt
    13201 aacaatttag cttttgaagc tggcttgaaa gctcacgtag atattacaga tgacgatttg
    13261 aaaactgctt ggaaatcttt ccatccagaa gtagaagcac aaatcatcaa attatcaagt
    13321 gaagatgaag caaaagatgt gaaaaaatca gcggatgatg gagatgattt ctctaaatta
    13381 gccaaagaca aatcaactga tacagagaca aaagaagatg gcggtaaagt taaattcgac
    13441 tcaacgacaa caactattcc tgcagaagta aaagaagcag ctttcaaatt gaaagacggt
    13501 gagatctccg atgttattac tacaacaaat cctacttctt atgcgacaga atactacgtt
    13561 gtcaaaatgg tgaaaaacca aaataaaggc aatgacatgg ataaatacaa agatcaacta
    13621 aaagatatcg ctactgaaac taaactaagt gacaatgcat tcacaactaa agtaatcggt
    13681 gaagaattaa aagatgcaaa tgtaaaaatc aaagatgatg cctttgaaaa tgtgttgagt
    13741 gcattcacaa caacttcatc aagtacgaaa gactcaagtg aaactacagc ttcaacaaag
    13801 agttctgaca caaaatcaac tgattcaaca aaagaatctt caactgaaga aacaactgat
    13861 tcatcaaaat aaa
//
LOCUS       DAZUKT010000072        13862 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000072 DAZUKT010000000
VERSION     DAZUKT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.074"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG816_002196"
     CDS             168..362
                     /locus_tag="ACG816_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921053.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG816_002197"
     CDS             631..930
                     /locus_tag="ACG816_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921054.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG816_002198"
     CDS             complement(1027..1461)
                     /locus_tag="ACG816_002198"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4921055.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG816_002199"
     CDS             complement(1693..2403)
                     /locus_tag="ACG816_002199"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4921056.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG816_002200"
     CDS             complement(2393..3253)
                     /locus_tag="ACG816_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4921057.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG816_002201"
     CDS             complement(3258..3896)
                     /locus_tag="ACG816_002201"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4921058.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG816_002202"
     CDS             complement(3911..4210)
                     /locus_tag="ACG816_002202"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4921059.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG816_002203"
     CDS             complement(4253..5737)
                     /locus_tag="ACG816_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4921060.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG816_002204"
     CDS             complement(5757..6218)
                     /locus_tag="ACG816_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4921061.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG816_002205"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG816_002205"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4921062.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG816_002206"
     CDS             7521..8291
                     /locus_tag="ACG816_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4921063.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG816_002207"
     CDS             complement(8564..9391)
                     /locus_tag="ACG816_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921064.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="ACG816_002208"
     CDS             9959..11194
                     /locus_tag="ACG816_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4921065.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="ACG816_002209"
     CDS             complement(12193..12453)
                     /locus_tag="ACG816_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4921066.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="ACG816_002210"
     CDS             complement(12480..13781)
                     /locus_tag="ACG816_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4921067.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4464 a         2833 c         2179 g         4386 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 ta
//
LOCUS       DAZUKT010000073        13792 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000073 DAZUKT010000000
VERSION     DAZUKT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.075"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            665..1534
                     /locus_tag="ACG816_002211"
     CDS             665..1534
                     /locus_tag="ACG816_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4921068.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1743..2768
                     /locus_tag="ACG816_002212"
     CDS             1743..2768
                     /locus_tag="ACG816_002212"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4921069.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2807..3757
                     /gene="manA"
                     /locus_tag="ACG816_002213"
     CDS             2807..3757
                     /gene="manA"
                     /locus_tag="ACG816_002213"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4921070.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3851..5554
                     /locus_tag="ACG816_002214"
     CDS             3851..5554
                     /locus_tag="ACG816_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4921071.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5556..7589
                     /locus_tag="ACG816_002215"
     CDS             5556..7589
                     /locus_tag="ACG816_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4921072.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7600..8040
                     /locus_tag="ACG816_002216"
     CDS             7600..8040
                     /locus_tag="ACG816_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4921073.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(8240..9367)
                     /locus_tag="ACG816_002217"
     CDS             complement(8240..9367)
                     /locus_tag="ACG816_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921074.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9370..10047)
                     /locus_tag="ACG816_002218"
     CDS             complement(9370..10047)
                     /locus_tag="ACG816_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4921075.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            10311..12077
                     /locus_tag="ACG816_002219"
     CDS             10311..12077
                     /locus_tag="ACG816_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4921076.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            12092..12529
                     /locus_tag="ACG816_002220"
     CDS             12092..12529
                     /locus_tag="ACG816_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4921077.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12542..13693
                     /locus_tag="ACG816_002221"
     CDS             12542..13693
                     /locus_tag="ACG816_002221"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4921078.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4651 a         2430 c         2828 g         3883 t
ORIGIN      
        1 aaagtcgtgt cacagtccct ttttccgaat aaacggtgtt caatcagctg tccttcggta
       61 ttagctcaaa aaacggcaaa atatgagaag ctatttttcc gttttgtatc tctaatactc
      121 aggtcaaacc gctgatttta caacctcttc cttaaacggt gttctcctgc caactcctcg
      181 gtcacaagtc acaatagtca acaaacctga gaagctgttt tttgaaattg ctccttgtga
      241 ctcggagtta aacggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagtttct
      301 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac
      361 tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca
      421 tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
      481 ttattacttg atgcagaaca gctttttcac aacctctttt tataagagtt tttactcatt
      541 aatttacaaa aaacaagcaa aatgcgaatt ttctcacatt ttttttaaca aaatcaaagt
      601 tattgcttga aagaagaaga atccatcgtt aaaataaagc ttgacgtaga ggggagcaag
      661 aaatatgaaa gattttcaat tgggggctgc cacctattta aaaagtgtag ccattcgggt
      721 gaaagaccga gataaaatga ttgattttta caagaacgtt gttggctttg atttgaaaag
      781 agaagaaaac gaattagcca ttttaggaac agaagaagct ggaagtgaaa tgttgctgct
      841 ggaagaaagt cctagagcga atgatcacat gggggtgatc aaaaagctga accgtttttc
      901 attgattatc cctagcacag aagaaatggc agatatcctt tgtcgtatcc gtaaaatgaa
      961 ttatccaatc aaagaagctt tagaagatca tggacgtatg gggattttgc tcgtagaccc
     1021 agaagagaat gaattggaaa tttaccatga agagaataag aacgaccaaa gagaaattcc
     1081 agaaacaatg gatcaagaag cattattaag aaaatctgaa gaaaaattca gtaaactatc
     1141 aacaggttct tattttgaaa aaatccattt aaatgttact aacttaacaa aagaacgcca
     1201 atttcttaat catgtacttg gtttgaaggt acataatgaa gaagagggca tccatgtatt
     1261 gaatgaagga gatttccatg ttggtttgac agaagctagc ggaggcgcaa ttgatttgcc
     1321 tacagatgaa gtgattggac ttgattttct gaaattcatg atcgatactg aaagcatgga
     1381 aggactgatt gctcacttgc agcaactatc agttgatttc ttcttagaca aaaagcacac
     1441 agtactcaca atctatgatg ctataggaat cgaatggtgg ttcgtcagtg agccaaaaga
     1501 aaagaataaa actgacattc gatgcgacgc ttaacttttt ctttcttaac gattgtcgca
     1561 tgtctcgtca ttttatgaac ggtcatacta tcgcttattc tagagataga aagagaaaat
     1621 agtataccgg gggaaactgc gcggtttttc gcgcagtttt tttgtgtaaa aaaggttgat
     1681 tcctcataga ttaagcttta agcttggact gtaagaatct gatagaaaag caggagaaaa
     1741 agatgtatcc acacacgaaa cagcttattt tgaaaaagat ggaagaagga ttttatccag
     1801 gtgtagcagt cgccttcata aaagaaaatc aagtagaaac atttacagcc ggctttgccc
     1861 aagtcgttcc tgaaaagcag ctgatgacag aagatctgtt gtttgatgca gcctcactga
     1921 cgaaagtcgt tgcaaccact tcattagttt tacgtctatt agaagatgga aagatagaac
     1981 tcgatcaacc gcttcataaa tatcttcctg aatttgaaaa tcagacaatc actttgcgtc
     2041 atttactgac acatacttct gatatcaaaa catggatacc aaacagagat aagctatctc
     2101 aggaagaatt gatcgccgct tatttgaagc tagaaccagg tgagcaacta ggaaaagaag
     2161 taaaatatac agatgccggt acgattcttc ttgggttgat gctagaggaa atttaccaag
     2221 aaactgcagt ggaaatcttt caagaacaag tattggaacc attaggaatg atgaacagct
     2281 tatttcttcc ttttccttcc aaaaaaatcg ttcccacaga acaactgcct tcgggtgagg
     2341 ttttgcgtgg acaaactcat gatccgaaag caagagtttt ggctgaacat gcaggtaacg
     2401 cgggtctctt tattgatttg aaagacagca ttacttttgt ccaaatgtat ttgaacaagg
     2461 gaaaaatccg taatggtcgt tttttagaag aagagacgat tgattcttta ctgcaagatc
     2521 aaacaccgaa aaaaaatggc tatcgttctt taggatggga cttaaaaaag gatgtagaag
     2581 acggtcatgc gattcttttt catactggtt atacaggtac gtttttcttg attgatataa
     2641 aaacacaaga agcgttgata tttttgtcaa atcgcgtaca tcctgtcgat catcgtgcgg
     2701 agtatctcat atgtcgagat gaaattgttg aaaacttttt gaaagagaaa gcggtattac
     2761 cgaaataatg ctataatagc tatgaaaaat gagaggagtt tgaattatgc aggaacctat
     2821 gtttttaaaa ccagtttttc aagaaaaaat ttggggagga agtcgtttac gttcagtctt
     2881 tggttttgat atccctaacg ataaaatcgg agaagattgg gcgatcagtg cgcatcctca
     2941 tggtgtcagt gtagtagaaa atggcgaatt caaagggaaa agattagatg aactatggtc
     3001 agaacacaag gaattgtttg gccatccttc agaaccagtg tttccattat tgatcaagat
     3061 cttagacgca gaagatgatc tatctgtaca agtacatcca gatgatgcct atgggatgaa
     3121 acatgaaggt gaattaggaa aaactgaatg ctggtatatc atcgatgctg aacctggtgc
     3181 agaaatcatc tatggtcatc atgcaaagac aagagaagaa ctagctgaga tgatcaaaga
     3241 tgggcgctgg gatgaccttt tgaagaaagt accagttaaa aaaggtgatt tcttttatgt
     3301 accaagcgga acgatccatg ctattggtaa aggaatcatg attttagaaa cacagcaaag
     3361 ttctgacacg acttaccgcg tgtatgacta cgacagaaaa gatgcgcaag gacaaacaag
     3421 agagctgcat atccaacaat cgatcgatgt aacaactgtc cctgcgaaaa caccagaatt
     3481 acaaatcaaa gaagtccgaa aagggaattc atcgatcgtc acttatttag aaactgagtt
     3541 tttcaatgta tatgagtggg atattaaagg aatcacatct ttcaaaaaac aagcccctta
     3601 tacattgatg acggtaatcg aaggagcagg agaccttgtc gttgacggaa aaacatatcc
     3661 attagaaaaa ggaacaagct gtattattcc aaatggcgtt tctgagtggg cggtccaagg
     3721 tgaactagca atcattgcgt ctgaaccagg caaatagttc ccagatagct gaaagcagca
     3781 gacggttttt cgtttgctgc tttttctaca taaaatccgt ccatcaataa atagaagtct
     3841 ggtgaaaaag atggaataca aatggaaagc aaacaacagc agtcgcgtgt ctatgaaaca
     3901 actgagaaaa gctggacatc acttaaatga aatgatcatg ccaaatctaa gtctgtttat
     3961 cgcttgggga atcttgtcat tagcaacaaa aggtgtgtct ggcgatttgc acaccacatt
     4021 agaagaggta tgccattgga tgatccagct gcttttaccc atcttgatca gttattcagg
     4081 cgggaaatcg gtagggggac aaaaaggggc agtcgccggt gcaattgctt ctttagggtt
     4141 gatcgctcgt tccaatgctc cacaaatcgt aggagcgatg attatcgggc cgcttgccgg
     4201 catcgtatat gaacaatttg tttctagatt ccatgtgaaa ttcaaggctg gatatgaaat
     4261 gcttgtcagc aacctactag taggactgac tggtagcgtt atttgtataa ttggaatcgt
     4321 ttttattgaa ccagttttaa atagtgttca cttgttgctc gcctccgttg tgtcttggct
     4381 agtcacaatg aatctgttac cacttgtcag tctgatctta gaacctctaa aagtactatt
     4441 tttcaataat gcaatcaatc atggtgtatt gacaccttta ggaatcgaat tctcacaaac
     4501 agtcgggtac tcttccttat ttttgatgga ggcaaatcct ggacctggtt tagggatctt
     4561 actttctatc ctttgttttg cagaaaaaca ggaaaaagta aatgcgagtg gagcattgat
     4621 gatccatgcg attggcggga ttcacgaaat ctattttcca tttgtcttgc tgcgaccgta
     4681 tctattttta gctgtaatgg ctggtggggc aagcggcacg ttgatatttc aatggttaga
     4741 tacgggatta gctgcacctg tatcgcctgg gtcactactc gtgatttttg ccaatgcgcc
     4801 ttatccacaa ctagctgggg taagtatagg gatagcaatc agtacatttg tgaccttcat
     4861 ttgtgcagcg atactattga aattaccaaa taaagaaaaa gagaacaaaa cggtcaaaga
     4921 aaggggagaa atcttggaac catctatcat tcattccgtc atttttgctt gcgactcagg
     4981 tatgggatca agtgctatgg gagccgccct gctgaaaaag aaattagcag cagaaaaaat
     5041 cgatctttct gtcggataca gctctatcta tgaattgaat aaccaagtaa atcagctggt
     5101 tatcgtgcaa catgaactag cgggtcttgc aagagaaaaa gtacctcaaa gtcaaatcta
     5161 tgtactggat cactttttgg aaacagatat cttgctgtca cgaataaaag aagcgatcca
     5221 atttccaaag cgggaagaaa aaaaagagat agcagatatc acagaacaaa aaacgtcgcc
     5281 tgctaatatt gtatttttgt ataaaaacaa cagacgcggc tcacaaacta tgggcatgac
     5341 gattttaagt caattagcaa aaaaagcgaa aaaacagttt gttatctcaa aagaatcaat
     5401 agaaaaaatc aaaatccaag aagaaacgat ttatatcgca caaagagaac tagctgtaac
     5461 tgaacagcta gaagaaaaga tccctcggct agttctagtt gaacattggg tagctacgaa
     5521 tgaatacgaa aaactcgtga agggagaaat ataagatgta tttatcgccc agagaaaaac
     5581 aattattagc agaatttctc agtagcccgt cacctgtatc tatccaaaaa atgatgaatt
     5641 tattgaaggt cagtaaacgg actatttacc gcgaattaga taatcttgaa gtctcattga
     5701 aatcggtaaa tgcttctttg aaaaaagcag ctagaggctc atttgtcatt gaggcatcag
     5761 aagaagtatt agagcaatta aaagaaaata cagatgggaa aagtaatgac ttatccactg
     5821 ctaaacgaca acacgcgatt ttagccgagt tgttactgac gacagagccg ttgagtcttg
     5881 ctcatttcat ggaatcatat cgcatcagta ataccacttt ttatgcagat atcaaacagt
     5941 tggaagaaag tattcgccaa ctgcctttgg aaatcattcg gaaccaggga tacgagatcg
     6001 aaggaccgga aaaataccgc cggcttctga cagcgaatgt attagaatta gaaatcaatg
     6061 aatatgaact ttttcatagt atttctttcg atgcatcact gaattatttt tttcgattcg
     6121 ttgatccgca acatctatct cttgcacgca aagtaatcgg ggaagaattg atccaaaaaa
     6181 agaccaatct aagtgataga aagcttgaac atttggtttt gatgctgaca ttgacgatgg
     6241 agcgtgtgac gaaaaatcat ttactgacaa atgagacata taatagtctg gcaaacaaag
     6301 aattactaca gacagctaaa cgattgtttt cacggatcgc ttctgagaca aaacaacttt
     6361 atccagtaaa tgagatcgtc ttttttgcag gattattaaa tgacttcacg aactcatttg
     6421 atcagaattt ctttgaagag acgttcgata cacagcttgc ttatttagta aaacaactga
     6481 ttcaagaagt aagtgaagag acgaatgtcc atttttatga agatgaaact ttgtacaaaa
     6541 tgctgttgac tcatttatct ggtgtgtttt caagagccgt tttacaagaa gagacactga
     6601 ccaatccgat tttagaaaaa atcatggttc aatacgagga agttgcgaat gcaatccgta
     6661 aagcagtaga gcggattttt caagaaaaaa aattatccga agaagagatc gcttatatgg
     6721 tccttcattt tgcgaattca ttggaacgaa gtccgaaaga aagcaatatc agtattgcag
     6781 gattttcgcc aagtggatta gcttctacta gcatgttgga actacgattg aaaaggtatt
     6841 ttcccttcat ccaacaaatt catttctatc ggattgctga tttaaaaaaa atcgatttac
     6901 aagaagaata tgatgttgtt atttctacat ctattttacc aggatacggt ggaaaatata
     6961 ttttagtctc cccgctactg ctagaagatg aagtcaagca gctaaaagaa gctttttcgc
     7021 atattgagaa gaaaaaaagg caaaaaatta cgtataacag caataacgag tggatgatag
     7081 aagatactta tgaggaaaca atgcatttca tggatcaaat caatcaatta ctcggtcgtt
     7141 tttttatcca agaactggat aatcctgaga cgatttccga aacgattcgc agcttatttc
     7201 cttattttcc tgaaggacta gtaacggatg aagaggtggt tcatcgccgg ctgcttaagc
     7261 gatatgaaca agcacctatc ggtattcctc atacaaatat ggggttattc catacatcca
     7321 gctcattgat ttctaaaccg atcttttgta ttttcaattt gaaacatccg ttgaccattg
     7381 aagggatgga tagacagcca gtcgatttgt cccgtatgtt agttatgctg gcgccttcgc
     7441 caatccaaga aaaagacggg attcttttag gaaagatcag tggagcaatc atcatgaatg
     7501 acttgaacac cgagatcttc cgctcaggaa ataaagaaat cgtttatcag ttgttagcga
     7561 aaatcctgat tgaagaaata aagaaatgaa ggtgaaaaga tgatacagct accagcagaa
     7621 aaaatattgc tcgatcaatc ctatcaaaat tggaaagaag cgttgaccga gatcggccag
     7681 tggatgaccc aacaaggggg aatcaaaccg gcatatatcc aatcaatgat cgaaagacaa
     7741 gaaaaagcga gtgtctatat cgggaatttc gtagccttgc cacatgggac tgacaaagat
     7801 gaagaagtag tagaagaagg aatctattta gtccaagtgc ctgatggtgt ggactttgga
     7861 acaaaagagg aaccaaaaat cgcgacactc ttatttgtag tggctttaaa aaaagaacat
     7921 cagttagcgg tgctccaaga tttagccttt ttctgttcag acatcgacca agtaatggcg
     7981 ctcagtgatg cggaaaccat tgaagaagtc caaatgattt ttgaacatgg acatatataa
     8041 ccaatgttca cagtaaaaaa agctgccttg catcaagtaa taacttcaac caagataaaa
     8101 ataagaggag ctatttttgt tgggaggttc ttattactgc ggatgcaagc ttttgaaaac
     8161 tatttattct agaaaaccgt ttatttgaaa taggggacgg tgacaaggtt tttgtcaccg
     8221 cccctattat ttttgtgttt taatagctct tttttatata gatagtgccc atacctatga
     8281 ataataaaac gatactccat ataccaagtg tcaagatacc attcatgaaa gtaacctgtt
     8341 ctgaagctag tggcgagaaa atatctcctc ctatgatcac attttgaaaa tcaacataag
     8401 aagttggcaa gtaatgactg attgattcta tccatccaat cgaagaaaac aagcgatact
     8461 ctgctaagaa aagacaaaga agtgtaccta aaagattgac tagagaatgt tcaaataaag
     8521 cagatagaag aaaactgatt gtacttaaaa agagcaccca caacaacagg aacagaaagc
     8581 tctggaaaag atactcatac atcggcagga ttcgtatctc ctgaccaatg atcgttccta
     8641 caggataacg gaagtctccc aaaccatttt tgattattac tagtatagag acgatcaaaa
     8701 atggcatcac gagacttagc atagctgcac cataataaat ggtaaaacgg atgctatgtt
     8761 tctttaccat actatccagt aatagattga ctagagagat tacctgcttt cttttttcat
     8821 aagagatgat ggaagaaaga aacaaggcgg ttatacataa aatcaaaaaa ctagaaatca
     8881 ttcctgaaaa aatattttcg tagtaatttg ctaaagagag atgcgctggc aagtcaaaaa
     8941 tagaatatct ttggatctga tgttctttca tataacggag caactcgaca cgtttttgtt
     9001 gttcaatgat tggaattcct tgtctttgac cagaaatcag tcgatccagt atatcttttt
     9061 cataatgata agttgcttcg accatttgct ggacattccc tgtgtttttt gcatgtaaga
     9121 ttgcttcgat cagtttgttg cttttctcta aactttccac aaattctttc tcattctcat
     9181 cttctttcaa cgtttcaatc aaacgttgca tctcttcata gttatgattc agttcatcga
     9241 attctgtata agcaagttta tgatcaaaaa aaagtgggaa tgcaaagagc acgaccaaca
     9301 atagaggaaa taaaaagttt actttgtttt ttagtaagag attgcgttca aatgtaagta
     9361 aagacatagt tacacctcat ataatttacg ataaatagct aaaatctctt ccatatttga
     9421 ggattctgtg acgatttctc cattttttag aaaacatgct ttagaacaaa tcgattggat
     9481 attcgcgata gcgtgggaag aaagaagaat cgtcttcccc tctttctgcc attgtctgat
     9541 gaggctattc aagatatcaa tacttcctgg gtctaatccg ttcaacggtt catcgaataa
     9601 catgacagat gaatcactga tcaagtaagc agcaagcaag acatgttgtt tcattcctag
     9661 agacattttt ccaatcggca aagtaaggta ctcagtcatc ttcaatcgtt ggaaaacagt
     9721 ttggcattct actttgctct gccaatagta tttgatcaaa gtcaaataat caacagccga
     9781 tagatagtcg gataactgat tcgttgattc tagaaaaaag aaagaccggc gataatctgt
     9841 ataagcagta ggagagatgt tgtgaaggga caattgatca taagttgttg gcagcagttg
     9901 ggcaatgcca ttcagcaaag ttgttttgcc aaatccgttc ggggcaatca aagcagctac
     9961 ttctcctgat tctactaaaa gattcaattg tcgaatcaca cgttttttac cacgtttcat
    10021 gttcatgttt ttgatcagta atgtcataga taggcctcca atataaaatt taataacata
    10081 atagaatgga aaacatctaa attaaatgct aacaaaagtt attgtttgca tattataaaa
    10141 aaattaaaat aacagtttta tttaaaattg aagttttctg ataccgaaat ctttttattt
    10201 tgctgacata ctcaaacctt tggcacaaag tttcgtgcca aagtacgact atttctcttt
    10261 ttagtaagcg gatacaatta gagtatcaaa aggagagagg gagagacaaa atggaacgga
    10321 cgattcccaa aaacaacatt tcgctaaaag cgagggtaca aaaattaggc agtactttat
    10381 caagcatggt catgccaaat attggagcac tgattgcctg gggtgtatta acggctctat
    10441 ttatcccaga tggttattta ccaaatgagg ctttcgctac catggtaagc ccaatgctaa
    10501 cttatctgat tcctttattg atcggttata ctggcggaaa agtaattgct ggtgaccgcg
    10561 gatcagttgt tggggcaatt gcaacgatgg gtgtgattgt cggaacagat attccaatga
    10621 tgctaggcgc gatgatcatg ggacctcttg gtggatatgc catcaagaaa tttgatcagc
    10681 tattccaaaa acggatcaaa tccggttttg aaatgctagt caataatttt tcagcaggtc
    10741 ttatcggttt tggattagcg ttattaggtt tttctgcgat tggtccagtt gtcgatgcat
    10801 tgactcaagc aatggcaaaa ggtgtagaga tcattttatc tgcccatttg attccattga
    10861 caagtatttt tattgaacca gcaaaaatct tatttttgaa taatgcaatc aatcatggga
    10921 ttttgacacc attgggaact gaacaagtag cagaggctgg aaaatctatt ctattcttgc
    10981 ttgaagcaaa tcctggtcca ggacttggcg tattacttgc ttttactttg tttggcaaag
    11041 gagcagcaaa atcttcagca ccaggagcca tgatcattca ctttttaggc gggatccatg
    11101 aaatctattt tccttacgtt atgatgaaac cgttattatt cttatcagtt ataggcggag
    11161 gaatgtcagg aagtttcatt ttccaattga tggatgctgg actaagagcg ccagcttctc
    11221 cagggtcgat cattgcgatt ctagcgatga caccgttcaa ttcttacctt cctgttattt
    11281 tgggcgtgat tgttgccgca gctgtttcgt ttggtatttc tgcagtaatt ttgaaagcgg
    11341 atgcgaaaga tacaaatgaa gacttcgaaa aatctgttca agagacaaag gcagctaagc
    11401 aagcttccaa aggtgcccaa ggtattcctc aaggagctac attctctggc gtacaaaaaa
    11461 tcatttttgc ttgtgatgca ggaatgggat caagtgcgat gggcgcttcg atcttgagaa
    11521 aaaaagtaca ggaagcagac ttgcctcaaa cggtgaccaa tcaagcaatc agccaactga
    11581 cagatgactc gaatacattg atcgttactc aggtcgaact acaagaaaga gcaaaacaaa
    11641 aagcaccgag cgcgcgtttt gtctcagtag aaaacttttt gaattcacct cgatatgatg
    11701 aaatcattca agaattaagc agcaagcaga aaacatcagt ttctgaaatt gagaatgagc
    11761 cagcagaaga gtttctttca gcaaacatca aacaaatcaa agagattcta cttgtccacg
    11821 atgatcgttc cggatcagca acaatgggga tgaccgtgct caaagatata ttagacaaga
    11881 accatttaac tatcccgtta agaaaagtca atatccaaga tttaaaagat gaacctgatt
    11941 cactgatcgt agccaaagaa agtttagcgg aaaaagtaca gatggctgca cctcaatcca
    12001 ttcatttatc agtcaacagc atggtcaatc cgcaaaaata cgaatcgatt gcacaatatt
    12061 tgaaacagac agcttaaggg aggaaaacag catgcaaagt ttgccattcg acatgatcga
    12121 gctcaatcga tcttttaaaa ccaaagaaga agcgatccgc tattgcggtc agatgcttgt
    12181 agaagcaggg tgcgttgatg aggaatatat cgatgccatg gtagatagag acaaaatgtt
    12241 atctgtttat atggggaatt ttatcgcgat tcctcatgga acagatgaag caaaaaaata
    12301 tgtgaaaaaa tccggcatct gtgtcgttca agtaccagat ggtgtagatt ttggtaccga
    12361 agaagaagaa aagattgcga ctgttttgtt cggtattgca ggagtcggag atgagcattt
    12421 gcaacttgta cagcaaatcg cactttattg cagtgatatg gacaatgttg tccagctagc
    12481 agacgcgctc agcaaagaag aagtaacgga aaatctagca attgcttaag gagagagaaa
    12541 aatgagagca gtacattttg gtgcaggaaa tatcggtcga ggatttattg gagaggtttt
    12601 ggcagctaat ggatttgaga ttactttcgt agatgtgaat cacacgatca ttgatgcttt
    12661 aaatgaaaga aaagaatacg aaatcgaatt agcagatgaa tcaaaagaaa aaatcactgt
    12721 aaaaaatgtc aaaggaatca acaatcaaac aaatccagaa aaagtaatcc aagaaatcgc
    12781 tgaagcagat ttagttacta ccgccattgg cccgaatatc ttacctttta ttgctgagtt
    12841 gattgcaaaa ggtatccaag aaagagaaaa ggaaggaagc acaacatctt tagatatcat
    12901 tgcctgtgaa aatatgattg gcggttcagc atttctagaa aaagaagtgt ataaatatct
    12961 tccggaaaca tcatttactg acaaatatat cggctttcca aatgcagcag tcgatcgaat
    13021 cgtcccatta caacaccatg aagatccttt attcgtccaa gtggaacctt tcaaagaatg
    13081 ggtcattgac gatagccaaa gaaaaaataa aaaaatccaa ttaaaagggg ttttatatgt
    13141 agatgatcta gaaccatata tcgaacgcaa actatttagt gtcaatactg gtcatgcgac
    13201 tgtcgcttat acaggtgcct tgttaggtta ccagaccatt gatgaagcaa tgcaagatgc
    13261 gttagttgtg gcacaattaa aatcagtttt gcaggaaaca ggaagtttgc tgattgccaa
    13321 atgggggttt gatgcagagc agcatcatgc gtatattgaa aaaatcattc atcgcttcca
    13381 aaacaaaaat atctctgatg cgatcacacg tgtcgctcgg acaccattac gtaagttagg
    13441 ttatcaagaa agatttacac gtccagtgag agaactacaa gaacataact tgacttgccc
    13501 acatttgacg gcaacaatgg ggattatttt taattactat gatcctgaag atgaacaaag
    13561 ccgtcaattg catgaaatga agatccatga aaacttggaa caattgattc aagaagtcac
    13621 aggaatcaat gatccgaaaa caatcgggaa tatcaagcaa aacgtgaacc gttatgccaa
    13681 gcaagtcgct taaaatagga tacgaaaaag gggaatgtga cgaaattttt gtcacattcc
    13741 cctttttcga ataactaggc tgtttgtcct tcaagatagg gcagatccaa tt
//
LOCUS       DAZUKT010000074        13785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000074 DAZUKT010000000
VERSION     DAZUKT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.076"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..1615
                     /locus_tag="ACG816_002222"
     CDS             188..1615
                     /locus_tag="ACG816_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921079.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1707..2072)
                     /locus_tag="ACG816_002223"
     CDS             complement(1707..2072)
                     /locus_tag="ACG816_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4921080.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2069..2434)
                     /locus_tag="ACG816_002224"
     CDS             complement(2069..2434)
                     /locus_tag="ACG816_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4921081.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2626..2874)
                     /locus_tag="ACG816_002225"
     CDS             complement(2626..2874)
                     /locus_tag="ACG816_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921082.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2873..3001
                     /locus_tag="ACG816_002226"
     CDS             2873..3001
                     /locus_tag="ACG816_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4921083.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3058..4752
                     /locus_tag="ACG816_002227"
     CDS             3058..4752
                     /locus_tag="ACG816_002227"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4921084.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4852..5148
                     /locus_tag="ACG816_002228"
     CDS             4852..5148
                     /locus_tag="ACG816_002228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921085.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5526..6659
                     /locus_tag="ACG816_002229"
     CDS             5526..6659
                     /locus_tag="ACG816_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4921086.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6690..7010
                     /locus_tag="ACG816_002230"
     CDS             6690..7010
                     /locus_tag="ACG816_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4921087.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(7039..7503)
                     /locus_tag="ACG816_002231"
     CDS             complement(7039..7503)
                     /locus_tag="ACG816_002231"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4921088.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7634..8887)
                     /locus_tag="ACG816_002232"
     CDS             complement(7634..8887)
                     /locus_tag="ACG816_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4921089.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8916..9233)
                     /locus_tag="ACG816_002233"
     CDS             complement(8916..9233)
                     /locus_tag="ACG816_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4921090.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9259..9579)
                     /locus_tag="ACG816_002234"
     CDS             complement(9259..9579)
                     /locus_tag="ACG816_002234"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4921091.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(10043..11458)
                     /locus_tag="ACG816_002235"
     CDS             complement(10043..11458)
                     /locus_tag="ACG816_002235"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4921092.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11475..12791)
                     /locus_tag="ACG816_002236"
     CDS             complement(11475..12791)
                     /locus_tag="ACG816_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4921093.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12941..13639)
                     /locus_tag="ACG816_002237"
     CDS             complement(12941..13639)
                     /locus_tag="ACG816_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4921094.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4380 a         2792 c         2363 g         4250 t
ORIGIN      
        1 tttatattgt taaaaattcg caatttattt tttaatagaa ttatctgttt tccaaatttt
       61 ataacataaa aaaccttttt taacggcatt tatatgcttt ttcagaaatt aactgatgat
      121 ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg
      181 ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg
      241 gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg
      301 caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc
      361 ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg
      421 caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt
      481 ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa
      541 agcttattca ttaaaaaaac aagtattagc ttattttaat gactgcggga tcgaaatcga
      601 ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta
      661 tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg
      721 tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat
      781 cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga
      841 ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg
      901 ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact
      961 attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca
     1021 gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga
     1081 atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct
     1141 gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa
     1201 tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca
     1261 gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag
     1321 cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt
     1381 tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc
     1441 accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg
     1501 ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc
     1561 tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc
     1621 tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc
     1681 tcttgataca cgtcaggctc acaacctcac ttattttgga catctacggg tccgatctct
     1741 ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata
     1801 tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa
     1861 cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct gactcggtcg
     1921 ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca
     1981 ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat
     2041 ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt
     2101 tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg
     2161 caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga
     2221 ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac
     2281 gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa
     2341 caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc
     2401 attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt
     2461 gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta
     2521 atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat
     2581 aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag
     2641 cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc
     2701 atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga
     2761 cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa
     2821 taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa
     2881 aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg
     2941 aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta
     3001 gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg
     3061 agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat
     3121 ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac
     3181 ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat
     3241 ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc
     3301 tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact
     3361 gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg
     3421 atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt
     3481 attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc
     3541 actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg
     3601 tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg
     3661 catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt
     3721 tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc
     3781 aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat
     3841 gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt
     3901 gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga ttcagctact
     3961 ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc
     4021 tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat
     4081 cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat
     4141 gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc
     4201 acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctatcgtcgt gcatcatcaa
     4261 ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca
     4321 agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta
     4381 gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa
     4441 gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc
     4501 gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc
     4561 aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa
     4621 agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt
     4681 cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa
     4741 atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc
     4801 aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct
     4861 tttgaacacc gtttaccaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     4921 cccaagctaa aactgcttct tatgtttttg acttggttgg acttattacc gcagatgcaa
     4981 gcttttgaac accatttacc aagaggttat gaaaaagctg ccctgcatca agtaataaga
     5041 gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt accgcagatg
     5101 caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt tcgagtatga
     5161 agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt aataccgaga
     5221 aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt gaaaacaact
     5281 attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac aactatttca
     5341 atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa tacataagac
     5401 tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt
     5461 aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg aaaggagcat
     5521 ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg
     5581 atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca gccatttgcg
     5641 gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg tatgttgtag
     5701 gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca ttgaaaaaag
     5761 ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc tgcctgaatc
     5821 acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg cttttcggat
     5881 ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga gttccttatg
     5941 cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa gttttatttc
     6001 tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg
     6061 atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta
     6121 tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta gataaagcgg
     6181 ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct ggaacagaat
     6241 tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga atggacggat
     6301 tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc acaatctctc
     6361 cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta actggtgttt
     6421 atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat atcgctgtca
     6481 agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg attgcagaag
     6541 gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa gcagcacaag
     6601 catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg aaaccttgaa
     6661 ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat acccatgaga
     6721 tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc
     6781 aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt ttctatattt
     6841 ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat ttttatttgg
     6901 ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac atttttattt
     6961 acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga gaatttttca
     7021 aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc
     7081 actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg
     7141 atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc
     7201 taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag
     7261 atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg ctcctccgac
     7321 gatggtttct ttttcatcta agaccacata ccattgagga atactttcct tattagcgat
     7381 tccagtacga atactttcct catacacaac agcaggaact ccccattttg atgaaaacca
     7441 attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac
     7501 caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa
     7561 aattagcaat gacaacaata gtgatgtaaa aaaaaaacgg aaaacaatca cttgttctcc
     7621 gttttttgat taatcaagca tctaaagctt cttttcttct ttcttggatc gtagctacct
     7681 tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt gacagcggct agtacaccac
     7741 ctgcaatact ttttgttgcc aatacaccgc caataattgg tggagttacc catggaggca
     7801 taacggtagc tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga
     7861 ccattggtgt caaaataaat ggaatgaaca tgatcgggtt caacacgata ggtaacccaa
     7921 acatcgttgg ttcattaata ttgaagacac ctggcgcaat cgacaagttt gttacaacca
     7981 tatatggttt gtttttacgt cctactaaat aaatagctaa cagtaaacct aaagttgcac
     8041 ctgttcctcc taagttgaca aagctgtcaa agaaaggttt gttcacgata aacggtgcgc
     8101 tatgcccagc tgcgattgca ttaacgtttg cttcaatggc tggcgcattg attgtttgca
     8161 tcaaaggatc gaccatattc gctccgtgta aaccaaagaa ccataagaat ggtgtgataa
     8221 atgcaagcag taaggcagat ggataagagt ttgcaagtcc cataaatggt tcttggacca
     8281 ctgtatagaa agaacctacg atatcatcaa caccgaatgc agcaaaaatt gcagcaacta
     8341 aagcaaacgc agagatagtg atcattgctg gtagtaatga agcaaatgct tttgcaactg
     8401 ccggtggcac accatcaggc attttgatga tcaatttttc atttccgctt aaacgtacaa
     8461 agatttctgt tgaaataatc ccaacaataa tagcaataaa caaaccgttt gagctcatac
     8521 ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt
     8581 cttttcggta gccgttagct aaatgataag caagcaagaa agcaatcaag acagaaagaa
     8641 tggcaaatgt accgttccaa gcagcagccc cgaatccttt ccatgattca ccaccaaaaa
     8701 ttgagttcat caattcttgg aacccgggaa tcggtaagtt attgatcatt actgctaatg
     8761 ctcctaagat cattaacggc atactcacca taaatgagtc ccgaatcgca actaaatgcc
     8821 gttgtgcacc aatttttgat gctacaggta taaaatgttt ctccataaat gcaacaaatt
     8881 tatccataaa tcttccctct tttctttact gtagattacg cgttcaatcg acgatacaga
     8941 tcaatgatct cttttgctaa atctgtaaaa gctatagatg tcatcagatg atcttgactg
     9001 tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg cttcttgtgt cagcatctct
     9061 gtttgagagt gatgtgcttg gactaaagat tcctctgcat ctttgatttt ttcatctgct
     9121 aaagtgaaat cacctttttt cgcagcagca atcgcttcca ttgcatcgct ttttgcattt
     9181 ccgccaaaca tgatcagacc catgatggct tctagatttt tttgatcttc catttattgt
     9241 atcctccgat aatttttatt atttgtttat tagtttttct gcttggtcga gtactttttc
     9301 accattcatc atgccatagt cagccatatt gattacatct aaaggaatgc ctttaggtgc
     9361 tagcttttca gcaaattgat tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc
     9421 tacattttta gattctagtt gattatccgc atctgaagca gatacagcaa agatgtctgc
     9481 ttccattcct tttgcttctg ctgccttttg catttttgtg actaggagac ttgtactcat
     9541 tccagctgaa catacaagca tgattgtttt cttttccatt ttaaacacgt tcctttctat
     9601 acttacactt gtatataagc aagtagcgtg ccaactttaa aagcgcttta aaaagttgat
     9661 atcatcacat ttaaggtcta taccaagttg catacacact aaaaaagaaa gcgcacacta
     9721 aacacacaaa ttaattttta ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac
     9781 acaaaacttg tcacaacttt tttcaaataa acggtattca aaagcttgca tctgcggtaa
     9841 taagtccaac caagttaaaa acataagatg ttgtttttac tttgctattc ttattacttg
     9901 atgcaggaca gctttttcac aacctctcaa taaacggtgt tcaatcagct gaccctgggt
     9961 attagctcaa aacgctggat tcacaacctc ttttatttca aataaccagt aaatacaatg
    10021 aacatctaca aaaaacttct atttatttat agatttcgtc aggcaaatcc aattctctct
    10081 tatcaatcaa agtctgatac cacgaagcag attttttcaa tgttctgctt cgttcatcgt
    10141 tcaattcgat tcgaactaat ccatatcgat tcttaaatgc attcattggt gacacacagt
    10201 cagtgaatgc ccataacata tagccttcgc aatttgcgcc ttcttctgtt gctttgatca
    10261 agtaatgcaa atgctgtccg ataaattcga tacggtaatc atcttgcacg attcctttct
    10321 catccatata ctgttcttca tattctcttc ccataccgtt ttctgttatc agccaaggga
    10381 tattatgata attttctttt agatacatgg ctatatcata catgattttc ggatagattt
    10441 cccatcccct ggaattattc attttcttcc caggtaaatc aaagacttca taatatcgtt
    10501 caggatggaa aggtgtctct tcattccatt gataacgagg ggcgcgaacg cgtttaggat
    10561 aatattgatt gattccaagg aaatctactg tatgatgctg aatcgtttct aaatcctctg
    10621 ttgcaggatc gaaataaatc gattgttttt gacacaactc caggaactct tctgtgtagt
    10681 atccttttac catcggatca aaaaatactc gattataaaa caaatcatac atataagcag
    10741 ctttttggtc ttctggagaa ttggatcgag catagaccat ttcaggattt aaaatacagc
    10801 caatccttcc accataattt ccgctgcggt aaatctctac tgctttagcc gttgctaaaa
    10861 ctttatgata attccacatc atccactttt ttgtgttttg ttcatgcggc cagcgaacag
    10921 catccaaata gcaacgcgtt tggggaacga ttggttcgtt gaatgtaaac cagtttttta
    10981 ccctatcacc gtaacgatca aaggcaattt gagcatactt agcataaaaa tcaactactt
    11041 ttcttgaaga ccagccatca tatttttcca aaagaaaagc tgggacttca taatgttcca
    11101 aacaaatcat cggttctacg tctgcttcga tcaaagagtc gatcatttca cttatatgct
    11161 gagcatattc ttcatctact atacagttct cataatcagt aaaaaaacga gcccaattga
    11221 tagaagtccg ataatgtgtc agatggatgg atttcatcag atccacatcc tgctggtatt
    11281 gttccataaa attagttgct acggctgggc catacccatt atgccaaaca aaatgttcat
    11341 ttttatacca gctatctata aacgaatcct gtccttcttt ttttcctttc caaccttccg
    11401 tttgccaagc agaagatgca gcaccaagaa taaaattttt tggaatagta attttcatga
    11461 tttcctcctg ttttttagat cccatcattt tccacacgat ttgctacttt tacaaatggc
    11521 agatagatca aaatagaaac aataatacaa acaagttgcg taataactgc accgatattt
    11581 cctgctgttg ccagataagc attgacgatc ggcggcattg gccaagggac cattacaact
    11641 gctttcgccg caaatcctaa agcggttgca aaataaccaa tcgttccagt gataagaggc
    11701 gttaagataa acggaatagc taaaattgga ttcagcatga tcggcatacc aaaaataact
    11761 ggttcattga tgttgaagat cccaggagca agggataact tggtgatttg tttcatatct
    11821 tcccgttttc caactaacaa tactgcaatc agtaaaccga tcgtgactcc agacccgccc
    11881 atactcatat acatatccca aaatggcata gtaataatgt tcggtacttc ttttccagct
    11941 tcaaaagctt ccgtatttac agcaattgcc gctaaaagca acggttcccg tatcggcttc
    12001 accatttgat ttccatggat accaatcacc caaaagactt gcgcaataaa catcaatagc
    12061 aaaatacccg gtaacccttg tacgacacct tctaatgggc gttgtacgac attgtaaatg
    12121 atatcataaa ggtacattcc tgtaagttct ttgatgataa atcccgcagc cgcgataccc
    12181 gatacagtca gaatcgtcgg gaataaagca gaaaaactag cagaaacatt cgttggcacg
    12241 gtatcaggca ttctcagctg tagtttcttc tgtttcctca accagcaata aagttcaata
    12301 gaaaggatgg agaccaacat tcccaaaaat aaccctttcg tatctgtata ttgtttagcc
    12361 aatacgtttt ctactataac agtctgattt tcattcacta attcataaac agttggatta
    12421 acagataaat aagacatcac tgcgatgata ccagggaata caccatccac tttattcagc
    12481 ttggctaatt cgatccctat cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca
    12541 tagctgatgg cagatgcgat aggctttaat tcttctaaaa aacgcaagaa acttatctgt
    12601 gctaagccat tttccgagtc aaagaccatt gctgaaaaca atgtgccaaa agcacccgta
    12661 ataattaccg gaatcaatgc agcaaatgcg ttttttatca ccatgatata ccttaaatta
    12721 ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt tatccataaa actcattcca
    12781 gatcccctca ttcctgcttc ttctccttga cgtcatctct ttttgtctat tagtcagcgc
    12841 ttacatttcg atgaacagaa actatttact cctctttttt accaaatata aaacaaaaat
    12901 tcatctgatg tatttatcat aaaatacatc agatgaactg tcaattagtt tctttttctc
    12961 ctattatttt ctgtaattct ctccgattta ttgccatatg gacagtcatt gcatcaaacg
    13021 cagcaagtgc attccgcctt tcgatggcct gaacgatctc tgtatgaata tcgatcgtct
    13081 cgattttcag atgctggata tcataatttt tattaaacaa agcaattgat tcattaatga
    13141 ttgggataat atgcagcaag gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat
    13201 cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg
    13261 ttaatttttt catttggacg atttgattct tcgttgcata ttccgctgcc aaacctgcca
    13321 tcttaggctc caataaatat ctcagttcaa ataagtctcg tatcatccga tcctgatctt
    13381 caatcaaaga aaaaccgagt gggtcattgg caatccctcg cttatcactg acaaatgtcc
    13441 ccgctccttg ccgaacttca aggatattcc tggaagctag agcccgcaca gcttctcgca
    13501 ccgtgcttcg ccccacctct aaataatcgg acaattcata ttcattcggc agtttatctc
    13561 ctacttgcaa ctgcatttcc tttaaatatc taaggatctt gtcagtagtt acttctacta
    13621 gggttttatt ttcttccaaa ccacatacct ccttttcatc tgatgtattt tttcgtaatt
    13681 attataacac gtttgaatca tctgatgatt ttttgtatac aaaattgatt aatccgttca
    13741 tagacataca aacgaagaag atatttacca taaaaaagac tagga
//
LOCUS       DAZUKT010000075        13749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000075 DAZUKT010000000
VERSION     DAZUKT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.077"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            365..535
                     /locus_tag="ACG816_002238"
     CDS             365..535
                     /locus_tag="ACG816_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921095.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            549..1151
                     /locus_tag="ACG816_002239"
     CDS             549..1151
                     /locus_tag="ACG816_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4921096.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1167..1844
                     /locus_tag="ACG816_002240"
                     /pseudo
     CDS             1167..1844
                     /locus_tag="ACG816_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2155..2652
                     /locus_tag="ACG816_002241"
     CDS             2155..2652
                     /locus_tag="ACG816_002241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS4921097.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2653..3069
                     /locus_tag="ACG816_002242"
     CDS             2653..3069
                     /locus_tag="ACG816_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS4921098.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3089..4633
                     /locus_tag="ACG816_002243"
     CDS             3089..4633
                     /locus_tag="ACG816_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4921099.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4776..5000
                     /locus_tag="ACG816_002244"
     CDS             4776..5000
                     /locus_tag="ACG816_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921100.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5015..5884
                     /locus_tag="ACG816_002245"
     CDS             5015..5884
                     /locus_tag="ACG816_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4921101.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5865..6599
                     /locus_tag="ACG816_002246"
     CDS             5865..6599
                     /locus_tag="ACG816_002246"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4921102.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6632..7540
                     /locus_tag="ACG816_002247"
     CDS             6632..7540
                     /locus_tag="ACG816_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4921103.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7537..8079
                     /gene="sat4"
                     /locus_tag="ACG816_002248"
     CDS             7537..8079
                     /gene="sat4"
                     /locus_tag="ACG816_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4921104.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8172..8966
                     /locus_tag="ACG816_002249"
                     /note="aph(3')-IIIa"
     CDS             8172..8966
                     /locus_tag="ACG816_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4921105.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9242..9373
                     /locus_tag="ACG816_002250"
                     /pseudo
     CDS             9242..9373
                     /locus_tag="ACG816_002250"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9449..9942
                     /locus_tag="ACG816_002251"
                     /pseudo
     CDS             9449..9942
                     /locus_tag="ACG816_002251"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10112..11008
                     /locus_tag="ACG816_002252"
     CDS             10112..11008
                     /locus_tag="ACG816_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4921106.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11107..11316
                     /locus_tag="ACG816_002253"
     CDS             11107..11316
                     /locus_tag="ACG816_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4921107.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11333..11605
                     /locus_tag="ACG816_002254"
     CDS             11333..11605
                     /locus_tag="ACG816_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4921108.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            11607..12470
                     /locus_tag="ACG816_002255"
     CDS             11607..12470
                     /locus_tag="ACG816_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4921109.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(12733..13470)
                     /gene="erm(B)"
                     /locus_tag="ACG816_002256"
     CDS             complement(12733..13470)
                     /gene="erm(B)"
                     /locus_tag="ACG816_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4921110.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(13595..13678)
                     /locus_tag="ACG816_002257"
     CDS             complement(13595..13678)
                     /locus_tag="ACG816_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4921111.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         4871 a         2136 c         2855 g         3887 t
ORIGIN      
        1 tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca tactatgata
       61 cgtgtttgtt tttctgtgct gtttagtgaa tgattagcag aaatatagag agtaagattt
      121 taattaatta ttagggggag aaggagagag tagcccgaaa acttttagtt ggcttggact
      181 gaacgaagtg agggaaaggc tactaaaacg tcgaggggca gtgagagcga agcgaacact
      241 tgatctttta agttgctatc tttttaaggt caatagagta tacttatttg tcctaatatg
      301 gttttagcag tataattgac ttggtgaata ggtcatttaa gttgggcata ataggaggag
      361 taaaatgaaa aaatttattt atcgagtttt agaaaatgac gaagtggtgg ctatttttaa
      421 tgagcaacaa tatgcgcaag attttatcgc ttacgaaaag acaatttctg ataagcaatt
      481 tgaaattgaa aaagtagata tagctgattg gttattgcaa ccgagagaat tttagaggtt
      541 ggttgaaaat ggctaaaatt ggttatgcac gtgtcagtag caaagaacag aacttagata
      601 gacaactgaa agcgttagag ggcgtttcta aggtcttttc agacaaatta agcggtcaat
      661 cggtcgaacg cccacaatta caagctatgc ttaactatat tcgtgaaggg gatatagttg
      721 ttgttactga attagatcga ttaggacgaa ataataaaga attaacagaa ttgatgaatc
      781 aaattcaaat taagggggca accctggaag tcttaaattt accctcaatg aatggtattg
      841 aagatgaaaa tttaagacgg ctgattaata atttagtgat tgaattgtat aagtaccaag
      901 cggaatctga acgcaaacga attaaagaac gccaagccca aggaattgaa attgctaaga
      961 aaaaaggaaa attcaaaggg cgacaaccta aattcaaaga aaatgatcca cgtttacaac
     1021 acgctttcga tttatttttg aatggttgtt ccgataaaga agttgaagaa caaactggaa
     1081 ttaatcgccg aacgtttaga aggtatcgat caagatacaa cgtgacagta gatcaaagaa
     1141 aaaaacaatg aaaagaggga tagttaatga gtacggttat tttagctgaa aaaccaagcc
     1201 aggcattagc ctacgcaagt gctttaaaac aaagcaccaa aaaagacggt tattttgaga
     1261 tcaaagaccc actatttaca gatgaaacgt ttatcacctt tggttttggg catttagtcg
     1321 aattagcaga accaggtcat tataacgaaa agtggcagaa ctggaaactt gaatctttgc
     1381 cgatttttcc tgatcgatac gattttgaag ttgcaaaaga taagggaaag cagtttaaaa
     1441 ttgttgctga acttctcaaa aaggcaaata caattattgt cgcaacagat agcgacagag
     1501 aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa tgccttttct aaagataaaa
     1561 cgtataaaag attatggatc aatagcttag aaaaagatgt gatccgaagc ggttttcaaa
     1621 atttgcaacc aggaatgaat tactatccct tttatcaaga agcgcaaaca cgccaaattg
     1681 ccgattggtt gatcggcatg aacgcaagcc ctttatatac cttgaattta caacaaaaag
     1741 gcgtacaagg tacattttca ttaggacgtg tacaaacacc gactttatat ctcatttatc
     1801 aacgccagga agcaatagaa aactttaaaa aagaaccttt tttcctgaat aattcatagt
     1861 tttactaata gtctttccta acattgattt aatagtcttt aaagtattct gcttcatcac
     1921 ccattaggtg atgaaaaaag aacccctttc tgatttagcg gacaaatact ttgacctaat
     1981 ctcagaagat ataaaagaaa aaataataaa atatccgatt tatactttgt ataaagaact
     2041 aaagtagtta gcaaatcatt tacagcaaat cactatgttc cacataccat tttacatact
     2101 taccttatcc acgacacatg ttgagatagt atcgaagata aagaaagttg ctccatgttg
     2161 tttttgcgag atggggaaat caaagccaca ttgacaacca tgatgaataa gcttgctttc
     2221 agtcataagc taatcctaga accacttttc aagtcagtta gccaaatcga tgaagaaagc
     2281 gaccgtgaaa gaatggacgc tattgataag ctaatggagc aactattgga agaacgcaat
     2341 acccttattg cactgatgtc caaaggtttc ctagaacctg ctctttttaa tcaggaacga
     2401 aatgttttag atagtgagat taaaaatctt actaccgaaa aaacaaacct ggtaacgaac
     2461 tctgcgagtg gggttttgcg agcaaacgag ataaaggacc tcattaatta tgtgtcagca
     2521 gataatttta atggtgacta cacggaagaa ctatttgaag aatttgtagt gaacatcatt
     2581 gtaaattcca gggatgagct gacattcaat ttgaaatgcg gtctttccct gaaagaaaag
     2641 gtggtgagat aaatggcata tattccatat ggatacaaaa ttcaagatgg agtggttact
     2701 gtcgatgaaa aggcagcagg tcaagtaaag gtattctttg agaaatacat atcaggacta
     2761 tcccttacag tggctggcga acaggcaggt attgaaaaga cacattctgt gatggggcgc
     2821 attttgaaaa acgtcaacta ccttggaaat gatacgtatc cagcaattat tgataaagag
     2881 atatttgata aagctgaaga agttagagat aaacgtgcaa aggatttagg acgagtggta
     2941 gagcttgccg ctttcacctc tccccctccc aaagaacgat tcaaaatgaa aaaggcagat
     3001 aataagatgc cagtggatcc ttttgaacga gcagaatact tatacagtct gatagaaagc
     3061 gaggaataaa gtgacagaga aaaatataat ggttattcct gctcgtaaaa gagtaggaag
     3121 tacagccgca aaagaaaaga taaagaaact tcgtgttgct gcctattgcc gtgtttctac
     3181 agaaacagaa gaacaaaatt ctagctatga ggttcaggtc tcacactata cagagtttat
     3241 aaagaaaaat aatgaatggg agtttgctgg catctttgca gatgatggta tctctggtac
     3301 gaacactaaa aagcgtgacg aatttaatcg catgattgca gagtgtatgg acggtaacat
     3361 cgacatggtt attacaaaat ccatcagccg atttgcgcgt aacaccctag actgccttca
     3421 atacattaga cagctcaagg ataagaacat atcagtttat tttgagaaag agaacatcaa
     3481 caccacggat gccaaaggtg aggttttgct gactattatg gcatctttgg cacagcagga
     3541 aagccagagc ctttcacaaa acgttaagct tgggctacag taccgatacc aacaaggaaa
     3601 ggtgcaggtc aaccataagc gatttatggg ctacacgaaa gatgaagatg gaaatttaac
     3661 tattgtcccc gaagaagctg agattatcaa acgcatctac cgagaatacc ttgagggtca
     3721 gagtctagtg ggcattggtc gagcccttga aaaggatggt attttaacag cagcgggaaa
     3781 accaagatgg cgaccagaat cagttaagaa gatacttcaa aacgaaaaat acatcggaga
     3841 tgcccttctg caaaagactg tcacagtaga ttttctgacc aagaaacgag tgaaaaatga
     3901 agggcatgtt ccccaatatt atgttgaaaa tagccatgaa gcgattattc ctaaagagtt
     3961 atttttgcag gcgcaagaag aaattcatcg gagaagcaat atctacacag gagaaggcaa
     4021 gaacaaaaga atttacagta gcaagtacgc tttaagtgcc atcaccttct gtggagattg
     4081 tggcgatatt tatagacggg tctattggaa tatccacgga agaaaagaat ttgtctggcg
     4141 atgcgtgact agaatcgagc aaggtcctga agtttgtaag aaccgaaccg taaaagaaga
     4201 tgaactttat ggtgcagtaa tgactgcgat taataagcta cttgcaggtg ggaacaatat
     4261 gataaagaca ctggaagaga atattcatgc tgtgattggc gaaaccacag aataccaaat
     4321 ttcagagatt aacaactcac tagaggaaaa acaaaaagaa ctaatcaagc tggcgaacaa
     4381 gggtcaagac tatgaccact tagcagatga gattgatgag ctgagagaca agcgacagat
     4441 ccttttagta gaagatgcct ccctcagtgg cgagaacgag cgaatcaatg agctgattga
     4501 atttatccgc aagaacaaat tccgcacctt agaatatgat gataagcttg taaggaagat
     4561 aatccagagc gttacagtct atgaaggcca cttcgtcata gccttcaaat ctggcatcga
     4621 aatggaaata tgaaaaccag aagtaaatag cccatgactc tacagtagag ttgtgggttt
     4681 tcttttgctt gctgattata ataatatttt aataactatt gtgtttatca accaaatggt
     4741 ttataatatt cttagcgtaa acttggaggt gctgtatgac tacggcagaa atgattaaag
     4801 aactgtgtga gcaaatgaat ataagtgttt ccgaacttgc tagacgtatt ggccagactc
     4861 cacagaattt caataaaaaa ttacaacgag aaacagtaac cttggatgag ttgaaggcca
     4921 ttgctgatgt gctaggtgtc aagtttgtgc aggcatttat tttaccagat ggagaagaaa
     4981 taaaaatatc taacgaataa aggaggatgt ttatatggta ggtaatgtta ttcaaatagt
     5041 aacagagaaa ttatcatctt tgccttttat agaaggcatc gtattagggg gctcacgtgc
     5101 aagaggcacc catacagaaa attctgatat agatatcgga atctattaca attcagactc
     5161 atttgacctg acagcgatta atcaaattgc tacagagttg gatgatgaga atagaaacaa
     5221 ccttgttgta cctcccggag cgtggggtga ttgggttaat ggcggtggat ggttagttat
     5281 aaacgggtgt catgttgact tgatattacg tgatataaaa cgggtggaac aaataatcaa
     5341 agatacggag caaggaattg ttactgccaa ttatcagacc gggcatcccc atggttatat
     5401 aagtgctatg tatcgtggag aattagcgat tagtaaaata ctatatgcta agaacgaaag
     5461 cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact gctctaaaga aaagcttgat
     5521 gaactttttt atatttgaag ctgaattctc tttaatgttt gtaaaagcaa atgcgggggc
     5581 agaggataaa tattatattg caggccatgt ttttcgtatt atttcatgct taaatcaagt
     5641 actatttgca tgtaataatg cttattgcat taacgaaaag aaagctataa aactgcttga
     5701 aacttttgaa tataaacctg aaaaatatgc cgaaagagtt aatcatattt ttgaagtact
     5761 cggtctttct ctttttgaat gctacgacat gaccgagaaa ctttataaag aagtgaatga
     5821 aattgtatcg gagataaata actttttaaa cgaggagagt tcagatgaaa gaaaacaaat
     5881 atgatgataa tatatttttt caaaaataca gtcaaatgag tcgctcgcag aaaggactgg
     5941 ctggtgcggg agaatgggag actttgaaaa agatgctacc tgattttaag ggtaagcgtg
     6001 tgcttgattt aggatgcggc tatggatggc actgtatata tgcgatggaa aacggtgctt
     6061 cctctgtagt aggtgttgat atttctcata aaatgctcga agtagcaaaa ggaaaaaccc
     6121 attttccaca gattgaatat gaatgctgtg ccatagaaga tgtggatttc ccagaggaga
     6181 gctttgatgt aatactaagt tcgcttgcgt ttcattatgt agcagactat gagaatttaa
     6241 taaaaaagat atataggatg ctgaaggctg gtggcaattt agtttttaca gttgaacatc
     6301 ctgtttttac tgctcatgga acacaagact ggtattataa cgaaaaagga gaaatactgc
     6361 atttcccggt ggacaattat tattatgagg gcaaacggac agctatgttt ttggaagaaa
     6421 aggttacaaa atatcataga acactgacca catatctaaa tacactgctt tcaaatagtt
     6481 ttataataaa tcagattgtg gagccacagc cgccagagaa catgatggat attccgggga
     6541 tggcggatga aatgcgacgc ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat
     6601 aatatagaaa aaataaacga ggagtatgta aatgagatca gaaaaagaaa tgatggattt
     6661 agtactttct ttagcagaac aggatgaacg tattcgaatt gtgacccttg aggggtcacg
     6721 cgcaaatatt aatataccta aagatgaatt tcaggattat gatattacat attttgtaag
     6781 tgatatagaa ccgtttatat ctaatgatga ctggcttaat caatttggga atataataat
     6841 gatgcaaaag ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct
     6901 tatgctattt gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga
     6961 taattaccta aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa
     7021 aagggacata gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta
     7081 tgatgattgc tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg
     7141 taaggaaatt ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag
     7201 aatgatatca tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta
     7261 taagtttatt gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg
     7321 gatggattcc tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc
     7381 ggtatccggt gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat
     7441 aacaaaatat accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag
     7501 cacatatgcc gctgatatag aagagaggcg ggaacagtga ttacagaaat gaaagcaggg
     7561 cacctgaaag atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg
     7621 aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa
     7681 agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac
     7741 aatactgata agataatata tctttactac caagacgata aatgcgtcgg aaaagttaaa
     7801 ctgcgaaaaa attggaaccg gtacgcttat atagaagata tcgccgtatg taaggatttc
     7861 agggggcaag gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa
     7921 aacttgcatg gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat
     7981 cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
     8041 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
     8101 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
     8161 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
     8221 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
     8281 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
     8341 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
     8401 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
     8461 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
     8521 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
     8581 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
     8641 aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
     8701 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
     8761 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
     8821 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
     8881 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
     8941 atattttact ggatgaattg ttttagtacc tagatttaga tgtctaaaaa gctttaacta
     9001 caagcttttt agacatctaa tcttttctga agtacatccg caactgtcca tactctgatg
     9061 ttttatatct tttctaaaag ttcgctagat agagttctat attagagatg taaagagttt
     9121 tggtgaagag gtttgtgagt atggctttta taaccagggt gcaatgagaa gcactaacag
     9181 cagctagctt aagaacgcat aggatatact ttttatggaa gtcacagagg aggaataaaa
     9241 tttgaacagg attaacagag taacctctat tcttattcag ctgcaatcaa aaaaaataat
     9301 tcctgccaaa gaaattgcac agcgatttaa tataagctta aggacagttt acagagatat
     9361 ccggacactt gaagaagccg gaataccaat tggatctgag gccggaaaag gatattttct
     9421 tgtagagggc ttcctacttc cgccggtaat gtttacagcg gcggaagtgg gtgcattgat
     9481 tacagcaggg aaatttttaa attgccatgg agatgaatca tttataaagg attttgattc
     9541 agctatgtat aaaatcaaat ctattttgaa gcatggtgaa aaaaactatg cacaggagtt
     9601 ggagaacagt attaacgtgt acagcacatc tggacagaaa aatacattgg cggataacgt
     9661 tattgcagca attcagactg caatctgcaa taaaagggta atatcaattc aatatcctgc
     9721 atcaggaggg caagaaccgg aaagcagaat gatagagcct atatcactgg ggttttatga
     9781 acagaactgg tacttaatcg gttttgcagg ctaagaaatg aatataggaa ttttcgtgtg
     9841 gatcggatta aaagcatttg catagaagaa gagctctgtc aaagccatga tggctctttg
     9901 gaacaaatat taaaacaaat gctttcatat aaaaaactat atgccctaca atgccgttgt
     9961 aaaaattggg gaaaagggat atattgattt tatatctttc tcaaaataaa tataaaagcc
    10021 ctttaaagag ggcttttata tattaatcac aaatcactta tcacaaatca caagtgatta
    10081 atcacaaatc acttgtgatt tgtgattctt aatgatacaa tattactata caaaaaaaga
    10141 atggggcgta gttatggaga aggaaaaact aaaaatactt gaagaattaa gacgtatttt
    10201 aaacaataaa aatgaagcaa ttattatctt gaataattac tttaaaggtg gtgttggaaa
    10261 gtccaaatta tcgactatgt ttgcttactt gacagacaaa ttgaatttaa aagttttaat
    10321 gatcgataag gacttacagg caacattgac aaaagactta gcaaaaactt ttgaggtaga
    10381 attgccacgt gtcaattttt atgaaggctt gaaaaatgga aacttggctt cttctattat
    10441 tcatttgact gataatttag acttgatccc tggcacgttt gatttgatgt tactgccaaa
    10501 attaactcgc tcatggacgt ttgaaaatga aagtagattg cttgctactc ttttagcacc
    10561 tttaaaaagt gactatgatc ttattattat tgatactgta ccaacgccaa gcgtttatac
    10621 aaataatgca atcgtggcga gtgattacgt tatgatccct ttacaagcag aagaagaaag
    10681 tacaaacaac attcaaaact atatttccta tttgattgat ttgcaagaac agtttaaccc
    10741 tggactagat atgattggtt ttgttcctta tttagttgat acggacagcg caacgataaa
    10801 atcaaacctg gaagaactgt acaaagaaca taaagaggat aatttggttt tccgaaatat
    10861 tatcaagcga agtaataaag taagtacttg gtctaaaaat ggcattacag aacacaaagg
    10921 ctatgacaaa aaagttttgt ctatgtatga gaacgtattt tttgaaatga ttgagcgaat
    10981 cattcaatta gaaaatgaaa aagaatagaa tcacaaatca caagtgatta atcacaaatc
    11041 acttgtgatt tgtgataggt gatgataaaa taaatagtaa gaagaaatag aaagaagtga
    11101 gcgatcgtgg gaaatttagg cgcacagaaa gcaaaacgaa atgatacgcc aatcagcgca
    11161 aaaaaagata taatggggga taagacggtt cgtgttcgtg ccgacttgca ccatatcata
    11221 aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag ttatggatca agccttagaa
    11281 gaatatatac ggaaatattt acctgacaaa ctttaaaagg agtgaaaatg aaatggcagt
    11341 tacgtatgaa aaaacatttg aaattgaaat cattaatgag ttatcggcaa gtgtttataa
    11401 tcgggtatta aattatgttt tgaatcatga actagatact aaaaatactc gtttactaga
    11461 agtgaatctt ttaaatcaat tagaagtggc acaagaagtt gatttatttc aacaaccatt
    11521 tgaagaatta caagctattc atgagtattg gcggtcaatg aatcaatatt caaaacaaat
    11581 tttgaataaa gagaaagtgg cttaacatgg cgaatattac tgacttcacc gaaaagcaat
    11641 ttgaagatcg tttagaaaag aatgttgaac gactaactaa aaatagacta gcggttgaat
    11701 cgccaaccgc ttttttactt ggtgggcaac cagggtcagg gaaaaccagt ttgcgatcag
    11761 caatttttga agaaacacaa gggaatgtta ttgtcattga taatgatacc tttaaacaac
    11821 agcacccaaa ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacacgtta
    11881 ccccttattc gaatcgcatg acagaagcac ttattagccg tttaagcgat caaggctata
    11941 atttagtgat tgaaggaaca ggacgaacaa cagacgttcc cattcaaaca gccacaatgc
    12001 ttcaatctaa aggttatgaa acaaaaatat acgtcatggc agtaccgaaa attgaatcat
    12061 acttaggaac gattgaacga tatgaaacca tgtatgcaga tgatccaatg acagctaggg
    12121 caacaccaaa acaagcgcat gatattgttg tcaaaaactt accgaccaat ttagaaaccc
    12181 ttcataaaac gggcttattt agcgatataa ggctttacaa tagagaagga gtgaaactct
    12241 attcaagctt agaaacgcct tccattagtc cgaaagagac cttagaaaga gaattaaatc
    12301 gtaaagtatc agggaaagaa attcaaccga ctttggagag aatagagcaa aaaatggttc
    12361 aaaatcaaca ccaagaaacc cctgaattta aagcaattca acaaaaaatg gaaagcttgc
    12421 agctacctac accaccaata cccaaaacac ctaaacttcc aggtatttaa accttaaaaa
    12481 aggaaaagag tagttaccaa aaaacggtaa ctactctttt tttataaaaa catttcctca
    12541 tgttggggtc ccgagcgcct acgaggaatt tgtatcgata agaaatagat ttaaaaattt
    12601 cgctgttttt ttctaatttc attttgaggt gaattaattg gtagttatta gaggtgccct
    12661 atacctaata atttatctac attcccttta gtaacgtgta actttccaaa tttacaaaag
    12721 cgactcatag aattatttcc tcccgttaaa taatagataa ctattaaaaa tagacaatac
    12781 ttgctcataa gtaacggtac ttaaattgtt tactttggcg tgtttcattg cttgatgaaa
    12841 ctgattttta gtaaacagtt gacgatattc tcgattgacc cattttgaaa caaagtacgt
    12901 atatagcttc caatatttat ctggaacatc tgtggtatgg cgggtaagtt ttattaagac
    12961 actgtttact tttggtttag gatgaaagca ttccgctggc agcttaagca attgctgaat
    13021 cgagacttga gtgtgcaaga gcaaccctag tgttcggtga atatccaagg tacgcttgta
    13081 gaatccttct tcaacaatca gatagatgtc agacgcatgg ctttcaaaaa ccactttttt
    13141 aataatttgt gtgcttaaat ggtaaggaat attcccaaca attttatacc tctgtttgtt
    13201 agggaattga aactgtagaa tatcttggtg aattaaagtg acacgagtgt tcagttttaa
    13261 tttttctgac gataagttga atagatgact gtctaattca atagacgtta cctgtttact
    13321 tattttagcc agtttcgtcg ttaaatgccc tttacctgtt ccaatttcgt aaacggtatc
    13381 ggtttctttt aaattcaatt gttttattat ttggttgagt actttttcac tcgttaaaaa
    13441 gttttgagaa tattttatat ttttgttcat gtaatcactc ctgaagtgat tacatctata
    13501 aataaataca gaagttaaac gatttgtttg taattttagt tatctgttta aaaagtcata
    13561 agattagtca ctggtaggaa ttaatctaac gtatttattt atctgcgtaa tcactgtttt
    13621 tagtctgttt caaaacggta gatgttttat ctacattacg catttggaat accaacatga
    13681 cgaatccctc cttcttaatt acaaattttt agcatctaat ttaacttcaa ttcctattat
    13741 acaaaattt
//
LOCUS       DAZUKT010000076        13606 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000076 DAZUKT010000000
VERSION     DAZUKT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.078"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..920)
                     /locus_tag="ACG816_002258"
                     /pseudo
     CDS             complement(222..920)
                     /locus_tag="ACG816_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            999..1175
                     /locus_tag="ACG816_002259"
                     /pseudo
     CDS             999..1175
                     /locus_tag="ACG816_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1351..1920)
                     /locus_tag="ACG816_002260"
     CDS             complement(1351..1920)
                     /locus_tag="ACG816_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4921112.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1994..2317)
                     /locus_tag="ACG816_002261"
     CDS             complement(1994..2317)
                     /locus_tag="ACG816_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921113.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2471..3283)
                     /locus_tag="ACG816_002262"
     CDS             complement(2471..3283)
                     /locus_tag="ACG816_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921114.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3610..4485
                     /locus_tag="ACG816_002263"
     CDS             3610..4485
                     /locus_tag="ACG816_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4921115.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4499..4822
                     /locus_tag="ACG816_002264"
     CDS             4499..4822
                     /locus_tag="ACG816_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921116.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4819..5571
                     /locus_tag="ACG816_002265"
     CDS             4819..5571
                     /locus_tag="ACG816_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS4921117.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SVHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5561..7732
                     /locus_tag="ACG816_002266"
                     /pseudo
     CDS             5561..7732
                     /locus_tag="ACG816_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7844..10015)
                     /gene="copB"
                     /locus_tag="ACG816_002267"
     CDS             complement(7844..10015)
                     /gene="copB"
                     /locus_tag="ACG816_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS4921118.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10035..12221)
                     /locus_tag="ACG816_002268"
     CDS             complement(10035..12221)
                     /locus_tag="ACG816_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4921119.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12221..12430)
                     /gene="copZ"
                     /locus_tag="ACG816_002269"
     CDS             complement(12221..12430)
                     /gene="copZ"
                     /locus_tag="ACG816_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS4921120.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12443..12883)
                     /locus_tag="ACG816_002270"
     CDS             complement(12443..12883)
                     /locus_tag="ACG816_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS4921121.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12957..13496)
                     /locus_tag="ACG816_002271"
     CDS             complement(12957..13496)
                     /locus_tag="ACG816_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4921122.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3831 a         2839 c         2415 g         4521 t
ORIGIN      
        1 aaatataagc tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac
       61 atgtttatag aatctaaata ataaattttt atgtaccgcc cctcaaagtg aactgaaccc
      121 caaaagttga actatttaat ggactgtttc cgatattcta ctggagatag tccattaaat
      181 agttcaactt ttggggttca gttcaaagag ggggggattt tttatcgttg tggaatattt
      241 aaataccaac gcttgtcatg aaaatcttgt gctccaccct tagtatttcc ttctggatcg
      301 tttgtcgcac gcatcatgac gtatactttc ttattaggga aatcacgcat attgaaagat
      361 acatgatagc caacatttcc taaagtatta taagcttgat ttacgtctgg tctataaatc
      421 ccatcagctc ttactctagc tagttctttt ccagtattgt agtccataat gaaaatatac
      481 tcgtatttat aattagcaat gtgccatcca gctacatgca agtttgcgtt ttcgatttct
      541 ccgaactgat caatgtgggc gtaatttgtt ccatctgtca gcgtagaatt tgcagcacct
      601 gctcgagttg gatcaattac tggtttatcc tccgaagtag ttggattatc atccgtaaaa
      661 ccatgagcca aatcataggc tagtttttct ttacttacac ccatttcaga aagataaccg
      721 taaggatctg tatgatcgcc ccaaatattt tgtgttaccc ataaatgcga tttgattcct
      781 ggttggttat aaggagtgtc taatgttaat ggaataccat atttcattgc tgaatctctt
      841 gccaattcaa cgtatacttt atagttcttt tcaaaagttt ctttatcatg tgtatgttgt
      901 aactcgattt gcacaggact tttggctcac gaggttgtcc tactctacat cttgctgata
      961 gatatgctga aaggaaaaca ccaacattat gttttgcttt ttcagctaga acgagacaag
     1021 caggaatacc tttagaagat atcaaaggtt ttttaggtca taaagatgtt tccactacac
     1081 aagtctatgc ccatatttct cccgaagtga aaaaaagatc aatgaatcaa ctagaaaact
     1141 atatagaaga gcaaataaaa aagcactcaa attgagtgct tttttaagct cactatcact
     1201 aaaactatca ctaattttat ttataagcga acatgtttct atcactgatt ttttaaatct
     1261 agcgaaaaac ccttatctta aaggcgttcg ttcaattctt tggcaaggtc ttcaaagcct
     1321 ggcttgccta gaagtgcgaa catggttttc ttatagctca tgtagaaaaa ttttcttgat
     1381 gatgatttta tccatcaatt gagattgact gttttgctct cctatgattt cagctatgat
     1441 tgtccgattt tgcggttttc ttcttaactg gtagttgatt ttcccagttt ccatcaactc
     1501 gctaagcata tccacgactc cgccaaatcc ggtctttttc gtaataaaac ctagtgctac
     1561 cattcgttca tctagatttt ttgctgtaat cgtcacacca gtaatgagcg agtgctgtaa
     1621 tgtttgggcc agctcttcca tcccttcagc attggatacg acggaagctg ttttgttaat
     1681 aggttcttcg tttatttctt ttggttcttt cctctccact ttcactgttt gacgaaggac
     1741 atctagttct tgcaagagcg tttcgtattg ttcttgggtc agcatcacgc cagctacttt
     1801 ttcacgatta aatatatata cgccagttcc cgcgtctcgc gcttgagcaa atacatccat
     1861 cggcgatcgt ttcacttctg tgatcgaaga tgtgggaact tctaatttct ttaattccaa
     1921 tggatttcct cctttactaa tcgcttttta ttatagcaca gactgacttg ttttgatctt
     1981 ttttccaaag gttttatcgg tttttgccta accagtaatt gatatcggta agaagtcttt
     2041 cgataccaaa tgaaggatgg tacagatacg ttttttgatc ttcgttatgg tctagtggaa
     2101 aaaaatgatc gatgatcact agatctgcat accgaggcgg cttatttcga gtacatacat
     2161 gtatagagat ccgttttcga tgtcttagcg ttttgttcag taccccagca agtcttcttg
     2221 cttcttggtg ttcatagcga aaaaaaaaga cgattttcac ttcaggaggg tggacatcta
     2281 aataccctcg ttcctggagt gttttcagta aaagcatccc ccactctact gcggggtgtg
     2341 caagtgcttg ttggatcgct agccgaaatt gatatagttt atctttggaa ttgggaaatt
     2401 tttctaatgt ttcctgataa taacgaactt cttctatcag ccggtcatgg ataccaatta
     2461 cgtaacgaag tcattcttgg taaagtactt ttgtcagctt tgatttgctt tcgctctcaa
     2521 attcgctatt cagccaaaac agcgaatcaa gcttttcagt cacaatcgtc aatttccgat
     2581 agtcttgtgt ctcttctatc cgtttccacc gcttttcagt aatctggtta cagctcaagc
     2641 gcaaaattgc tagataattt ttttctttta ttggcaaaat ctgctcctct ctaaatagca
     2701 attcaggaaa caattcaaag aatagtgttc gactgtctcc taaaatctcg atttctgccg
     2761 gatcctttct ttgcttcaat aattggatga acatctcaga aaaggactgc tctccatcat
     2821 ctgcaaaaaa atgcggaaaa tctttcaaaa agcaaaacag cagatatctt acttttcttt
     2881 ctgaaccaaa gatctgtgct tttttataag aaaaagaaag gtggtactcg ccaaaccact
     2941 ttctcaatcg ctcttgcgta gaacgatcgt acttctgctc gtaaggttca ttcaatagca
     3001 gatttttcag taaacaaaac gggggtgatt ggatcagaaa aacaaacaag agataaggtt
     3061 cgctatatcc ttgatcataa actaactgaa cggttttctt atgaagaatc agctgaatgg
     3121 gacagtcgct ttccgccaaa cgtttcagat gccgatccac tgttttaaca tcgattttca
     3181 gttcttcagc taaatcagct tttcgccaga tcaaatcttt cattagattg attagtgaaa
     3241 ataaattctg tgcatttttt gataatagtt tttgccatct catcgatcat ttctcacctg
     3301 atttctaaac atattgtttg atcaaaatgg gttcactcct aaaaagataa aaaactccct
     3361 ttctatttat ttaaatacgc aaatcttcct cattattttt tattttactc aatttaatcg
     3421 tattctcatt tcaaaacgaa tgtactttaa aaaaacataa taagatcact ttgcttgtag
     3481 gatgtattcc cttccattac aatgaaataa atagcgattt tatgagaagg tgatgtgaaa
     3541 atggaagaaa aaatagaaga atccaaacaa aaaatcgatc agttgatata agaattagaa
     3601 aaagcggtca tcggcaaacg ttcaactatc caattgatga tcgtggccct attagcaggt
     3661 ggtcatgtct tattcgaaga tattcccggt gtaggtaaaa cgttactggt caaagcacta
     3721 gcaaaagctg tacagggaac cttcagcaga gtccaatgta ctcctgatct gctgcctagt
     3781 gatattcttg gtttttccgt atacaattca caatcaaaag agtttgaatt tcgtccggga
     3841 ccgatcttta ccacgatctt gttagcagat gagatcaatc gtacgactcc tcgtactcag
     3901 tcagcacttt tagaagcaat ggccgagaat catgcaacga tcgacaacaa tacataccct
     3961 ctagacaatc atttttttgt cttagctacg caaaatccaa tcgagtacga aggaacatat
     4021 cctttgccgg aagcacagct cgatcgtttt ttattccgac tacaaatcgg ttatccttct
     4081 tttgatgacg aacttttgct acttttggat aaattcgaaa aagtgttgga taacttaaat
     4141 gaagtattga acagttatga aatagaagaa ataaaaacaa atgtggcgaa tgtctttgtc
     4201 agtcccgaag tggcatctta tgccttgcag ttagtgtcag caacaagaag tcatgctgcc
     4261 atccagcttg gcatcagtcc acgaggaagc ctttccttta ttcaggcagc aaaagcttat
     4321 gctttaatcc atgggagaaa ttacgtcacg cccaaagatt tgcaggattt agttccttac
     4381 gtgttttccc atcgcctgat tttttacgat cgttcattga aggaagacga aaagaaacaa
     4441 atcattcata ctcttgttgc acaaattcct attcctgtga ggtagattga ggtagacaat
     4501 ggaaaaaatc aaagattatt tcattaattt tgtgttattt ctggtatatt tcatcattgt
     4561 gctttttgct cttgtattca acaattcttt aggctggttc ttgttggcct tttttactct
     4621 ttatgtatca tggagcttga tttcattaag cagctttcta aagaaacttt cggttacttg
     4681 tacattgcag ccactcgttt atcgcaaaaa aagtatgcag gttatttttt ccatagcaaa
     4741 aaagagaact ttttctttac ctctccctcg tttacaaatc ggcttcccag caatctttgc
     4801 agaaaaaaac aagagatttt gattcttaca aataaaccgc aagaagtcca attgctttgg
     4861 cagccaaaag aaagaggatg ccatgaaaca ttaactgtca tttttacagc ctatgatagt
     4921 ctaggacttt tcagaaaaag tgcaaaaaga gagatctctt ttcctgtgac tgttctgcct
     4981 gctttttgta agattcaagc agaaaaatta cagagagttc tggaaaaaaa acagcagctg
     5041 aacgcgtacc aaagaggtct tgattttcgg gaacatcgcg cttatcgtcc aggtgattct
     5101 ttcaaccgaa tcgattggaa attgtctgtt catactcaag agtggttata ccgtgaatac
     5161 gattatcaag aagaagaatg ttctcctctt ttttgctttt ggggttcgcc cagtccaaaa
     5221 tttgaacctc tgctagactt gtatttttct ttatggcatt tgagaaaaga aaagcagccg
     5281 gaacttctga tattaggcaa ccgcgccttt catggcaaag accccggtca tacctatttt
     5341 gcttctttgg aagctggcga tgagaaagct tttttcgcac acttgaaaaa atatgcaaaa
     5401 aaacaaatcg tcctctttgt tcctgagaat tctcctgaag tctcagaagc tattgaaaca
     5461 ttgtcaaaag atcgaacggt ttatcttgtt tatttcgtag aaaaacaact catggtccaa
     5521 gaaaaagaca agatatgttc attgccagga ggtgaatgga ttgacgatta aacgggaaaa
     5581 atggctgcgt ggcgtttact cctttgtgat actaagtgta ggtgctccga tatttgtagt
     5641 agctaatcaa ttgcaaaaca gctaccttct tatcctaact attggtgtcc tatgccttat
     5701 tgtctgctgt attcctaaaa aatgggtacg tttgctgctt tctttgccag taatgatagg
     5761 ttgtttgttt ttatattttc cttcgaagac attttccata ttatggatct tacagttttt
     5821 ttcgtcagtc acagaggaat tcttccatct atcccaaggt aaactttatt atttgccaga
     5881 aaagacagct ttttttctca taatgttgct gatctattta tttattacat tgaccgtgga
     5941 ttacgactat tggtacgcac cctttctttc tttgatggct tacttcatga tgcttaccgt
     6001 ttttcggaaa aaagatttac tgtacgaaat gattgcggta gtaactgttt tgttcgtgta
     6061 tctggcagca aggcaatttt tctccatccg gttgttgtcg ggaaacactc gattccagtc
     6121 attgactaca gttctgctta tttgtttctt aacttttagc gctatcttgc ctttatatct
     6181 tccgaaagta caccaatctc tcgaggaaac gagtgaaccg atccgagaat atcttaatga
     6241 tgaagggctt tatgacacac tccatgagta tcaattaacc ggaagtgcaa gaacaggctt
     6301 cagtgagaat gatgaacagc ttggtggacc tttatatgat aatggcgaag ttgtttttac
     6361 agcgaaccaa aaaggaaatg gctattggaa gatcgaaaat aaaaatcatt acaccggttc
     6421 tggatggata gaagatcagg acgcacccat ccaaccaatc caagaattcc ctgttgacat
     6481 cacgagctac caaaccgtta tagaagacaa agaaaacatt acattgaccc taaaaaaaca
     6541 agttttctta ccttatcctt atggaaatac taccttgact gatttttcat tagctagtgg
     6601 acaaacgctt tactatttgc ctgaaagcca gcgattcatt cctgtcccga atgaatcgac
     6661 acgttctgca actttacaaa cagaaaggtt agtttttacg agagaagaat taaaaagcag
     6721 tcagttaaaa gctgatgatt tagaaaatca gcagacaaat cttcaattgc caaaaagttt
     6781 gcctaaacgt attgaggact tggcggtcga attgaccaaa aatgaagcaa ctttaattcg
     6841 taaagtcgaa gctgtctaac agtatttaaa aacaagcgga aatttccgct attccaaaac
     6901 ggataccggg catactccaa aagaacacga ttatgtggat tatttcctct ttgattcaaa
     6961 agtcggctat tgtgacaact tttcaacttc tatggtcgtc cttcttcgaa cacttggtat
     7021 cccagctcgc tggacaaaag gttttacaaa tgggctcaga acaggcacag ataatcaagg
     7081 gaatgaggaa tacagcattc tcaatagtca tgctcattct tggccagagg tatactttcc
     7141 tggatttggc tggcttccat ttgaaccaac cccttcattt gccaatccaa tgggttcaca
     7201 accagttgct gaaacaaccg aaaccgtcca gcctgctatc gaacaaccaa atgaaagcga
     7261 aaatatggca gaaactattt ccagtgagag atctgaggtc attccatcag aagaaacaac
     7321 actcacacca gcttataaaa tcgtcttatt cagccttgga gtgtgtttat ccgtcttgtt
     7381 gttctttcta ttgttattca gaaaatctct gcgctggaca agttatctta tctatcttca
     7441 tttttttgct tctactaata cgattgcata tcaaaaactt cttaaccctt tggaaacatt
     7501 ggtaccgcgc aaacccgcag aatctttgca ggaatatagc ctgcgaatag aacaagaatt
     7561 caagctagat ctgcgtttca ttcgattaac tactcaatat gaagcagaaa tatatggacg
     7621 acaagcagat ccggtaaaat tcgataaaaa aacctattta gaaataacgc atataataag
     7681 aaaaatggta tacaaaaaaa ttctgacaaa agggagccgc ttcaaaaaat aagataaaat
     7741 ttctggagaa tctacttctg gaatgccgag aaccagctga ttgagtatcg attactcgaa
     7801 aaaaaggttg tgacacgttt tttgtcacaa ccttttttca ttattatctg attttcaatg
     7861 tcaaagcatt tagagctaca actaccgtac ttaacgacat caagacagca ccaactgctg
     7921 gacttaatat gataccaatt ggcgcaagaa ttccggctgc tagcggaatt gcaagaatat
     7981 tgtaacctgc cccccaccag aggttctgga tcattttctt tcgcgtttgc ttggctagat
     8041 caagaaaatg caaaatatct tttggatcgc tgtttgtcaa tacgacatcc gcagaatcga
     8101 ttgcgatatc tgtccctgca ccgattgcca tcccgattga agcacgggct aaacttggcg
     8161 catcattgat cccatcaccc accatgatta cttttttccc ttggtcaagg tatttctgga
     8221 caacagcttc tttatcatct ggtaataaac cgccataata ttcttcgatc cctaaataat
     8281 ctgctactgc ttgggcagcg tttttattgt caccagtaag catgacagga gtgatttttc
     8341 tttctttcaa tgttcggata aattcttttg cttctgtttt tactacgtca cctaaagcaa
     8401 cgattgcagc tagatgattt tctaaaatca ggaagctgat ggtatttcct tgttcttggt
     8461 aaggtttcaa taatgcttgt tctacgttta atccgagacg ctcaacttct ttttcattaa
     8521 ctattttcac ttgttgtttt ttgactgttg cctctagccc gactcctgaa agatttttta
     8581 gattttcagc ttgataaggc ttgatttcat gttctttcaa ataattcata atgccaacag
     8641 ccaatggatg attggcattc gcttccaagg ctcctagata ttgaaggatt tcatttttat
     8701 tgaacgcatc atcaagtact tcgacacccg ttacagtaaa ttttccttct gttagtgttc
     8761 ctgttttgtc cagcatgatc acatctagat cattggcttg ctccagtgca ttgcggtttt
     8821 tcaaaagtaa tccgttttta gctgctattg atgtggaacg agcaacaact aatgggatag
     8881 ctaaacctag agcatgagga caggcgatga taaagactgt taccatacgt tctaatgcat
     8941 taggaagatc tgtcaagaac aaccaggcga tgaatgccag gatacccaca attaatgcga
     9001 cataaaacag ccattttgcc actttgtcag ataatgactc tagcttggat ttttctcctt
     9061 gtgcttttcg caccatttcc ataaccttcg ctagataacc attttcacca gttccagtta
     9121 cttcgatttc gatcgttcca tccccattga tagaaccgcc aatcactttg tcattggctt
     9181 gttttttgat tcctcttgat tcgccagtca cagctgattc gtcaacaatt gtttcacctt
     9241 tcaagattac accatctgtt ggcattttat caccagaccg gacgatcaaa cgatcacctt
     9301 cgtttacttc tttcaatgaa actgtctctt cttttccatc tttcgtcaag cgtttcactg
     9361 attctggcag taattctgct agtttttgta aagcattgct ggcattcgac acagcattca
     9421 tttcaatcca gtgacctagt agcataataa cgatcaaagt agccaattcc cagaagaaat
     9481 ccatgacgtg tgtatgtgga ttgagcagat ttgcgataaa cgaatagaca ctataaatat
     9541 atgcaactgt aatccccatc gcgatcagtg tcatcatagc aggacttttt tgtttcaatt
     9601 ccatcttagc tccacttaaa aatggctgac cgccatagat aaatagtata gttgctaaga
     9661 tcaatacgag ccaatcagaa ccaggaaacg ttacttgaaa tggaaattcc attcccatca
     9721 ttggtgaaaa aagaatgatt gggatagcta ggataagcga tagccaaaat ttttgtttga
     9781 aattccccat gtgcatggaa tgatccatgc cgccgtgttc gtgatccatg tgcatcgaat
     9841 ggtccatttg tttgctgttt gctatatctc catggttatg tcccatgtgc atggaatgat
     9901 ccatctgact atggtcatgt ttcatctcca tctgtttttc atgagattct gtactttctt
     9961 tgtttactac ttctttttta tgctcatgtt ttttctccat atcgttccca ttcattttta
    10021 ctccatccct tctcttactt tttcatgtgt cgatttaagc acagggagtt caacaataca
    10081 ctgacggaac taaacgccat cgctcctcct gcaatgattg gattcaaaaa gcctagtgcg
    10141 gcaaaaggaa taccaatcgt attatagaca aaagcccaga aaagattttg tttgattttt
    10201 ctcaatgtca acctagacaa ctcgattgtt tgtgcaatgg atgccaaact gctattcatc
    10261 agcgtcacat cggctgtttc catcgcaatg tccgttccac ttcccattgc cataccgaca
    10321 tcagctaatg ctaaggccgg tgcatcattg attccatcgc ctgccatacc gacttttttg
    10381 ccatctttct gaagtttttc tacgtaggct gctttatcct ctggaagtac ttcagcaaag
    10441 atatgctttg gatcaatacc aacttgttta ccaatcgttt cggctgccag tttgttgtct
    10501 cctgtcagca taaaaacatc taatccttta ttctggagct gcttgattgc ttgtttgact
    10561 tctagtttga tttgatcagc cacggcaatc aacccgatca cttgcttttc atcagctaaa
    10621 aacatgactg tttttccctg ctgttctaat tctaacgcgt gttcttggta ttcatcaaat
    10681 gataggttca attcaattaa tcgtttacgt gtacccgcaa aatagcgtac tccgttaatc
    10741 gtaccgctga ttccagcacc tgggtgggct gtaaaatcaa tcatatcgta tgttgctgct
    10801 tcttgcagtt ttccgtattc cacgattgct ttacctagtg gatgttcaga agcttgttct
    10861 aaagtataaa aaatagatag gacttcttta gaaccgacta gatctgtaac tttgggcttt
    10921 ccttctgtaa tcgttccagt tttatccaaa acaatgctgt ctaagtgagc agctgcttct
    10981 aatgcttctc cgcctttaat taaaattcca tttcgtgcac caacacccgt tcctaccata
    11041 atagctgttg gagtcgctaa acctaatgca catggacacg caatgactaa tacagagaca
    11101 ctatggatca atgcgagttg ccaatcacca gtgatcaaac ctgtagctat gagcgtgata
    11161 aacgcgatga ctaatacgat cggaacaaaa atccctgaga tccgatctgc gattttttgg
    11221 attggggctt tgctcccttg agcatcttcc accatttgaa tgatttgcgc taatactgtc
    11281 tgcttgccga cttgtgtaac ttccatgtga agtagaccat tcgtattgat tgtcccgcca
    11341 aagagcgtgt catccggatt cttttctaca ggaagatttt caccagtaag catactttca
    11401 tctatcgcac tgcttcctga aataattcga ccatctgctg gaatctgctc accaggacgg
    11461 atgactagtt gatcaccaac gactacttct tcaatcgcta acgtttcttc tttcccgttt
    11521 cttatcactt gggcggtttt tgtttgaaga gacatcaatt gtttgattgc attacctgtt
    11581 ttcgtttttg ctgtatgttc caaatatttg ccgagcaaaa tcagcgtgat gatcatactg
    11641 ctgctttcaa aataaagatc ttgggggtgt ccacggaaaa atccgttgta aatgctcaat
    11701 gcaaaggcag ccgatgttcc aattgctacc aatacatcca tatttggtgc tttcgtcttg
    11761 attgcatggt acgcaccttt ataaaaacga gcaccgacat aaaattggac aggagcagac
    11821 agcactaact ggacgattgg aaaatgaaaa aagtgaacaa tcgccgcatg gcttcctaac
    11881 atcatagcaa tcattgacaa catcaacggc aaagtcaaaa ccgtactcaa tataagatcg
    11941 aatagcattt ttttcaaata cgcttgcttt tcttctgcaa ttttttgttt gtgggcttca
    12001 tcgtacaaaa ttgcaccata gccgatgttt tctacgcttt ggattaattt ttcagtggtc
    12061 gtcccttcat atttgacact tgctttttca gtggccaagt tgactgtggc attgaccaca
    12121 ccaggttgtc cgttcagctc tttttcgatc ctagcagaac aatttgcaca agtcatacct
    12181 gtaatgacga acgtatccat tttggtttct gctgttgcca tcaaatcacc tctgctttat
    12241 aacccaatcc attgattgct tgacaaatct tttcggattg aacttttgtt tcatcaaact
    12301 tgaccgtacc gtttgctttt ttcaaattta ctttcacttt ttgcacgccg tcaagtgcgc
    12361 ttactgtttc ttctactctt gctgcacaat gattgcagct cataccttcg attgaaaatt
    12421 gttgtttcat tttttcttcc tcctattgat gttgtttaca ttcacattga cctgggatac
    12481 agttacattc gatcgtatct actggttgct tttgttccag ttgtttcaaa atctcattga
    12541 tatcttcttg agtcaaagtg gcttcttcta ctagatccgc aatcgtctct ccaatccttt
    12601 ttgcacagat atgggaaaaa agattttccg tcgcactgcg aactgtttcc atctccgaga
    12661 cagctggata ataaatgaat ttttttcctt cttgttctgt ccagatcact tcttttttta
    12721 ccaaacgacc cagcaaagtt ttgatcgttg ctggtttcca atccatcgaa tcagctaaga
    12781 tctgcgtgat ttcttgggct gtcgtctgtc caagtgtcca aataacacgc atgatctccc
    12841 actctgaatc actgatcttt actggcgttt tgactttttc catttatttc acacctttcg
    12901 tttacaaatg taatcattat cttatgaata cagattacac ttgtaaacaa aaactgtcaa
    12961 ttatttttgc tctctatttt caatttagtt aaaaattctt gtttctctcg tcttgtttta
    13021 aggatgatca gtttatttct tgcattagct acttggatca gctgctgtat tttcggttca
    13081 ttgttttctt caaaatgcca gataaaagat aaaaattcta agtattccct gtttagtttc
    13141 tccgaaaatg attcaggcat atctggccga gattttttgt ttatctttct ttttagagaa
    13201 cgccaaacta ctcggtagat ggcgagataa cgcggcactt taagccaaat gatcttatct
    13261 gcttctttaa tcctttcctc taatgtagaa gtataatttc cttcaacgat ccatgacgga
    13321 ttagatacca taaacaattg ctgttcttgc aaaaaccact tttttgcctg aatactgtag
    13381 tctgttgtat gccataaaag gtctagcggc agtagcggaa agtcggtgat ttctttgatc
    13441 ttccttgcga gggtcgattt tcccgaccca ctttgtccaa tcacgatgat cttcattctt
    13501 ctccctcact tctctttgga atttggttcg atcataccac ttataacgcc gcaaaaaaac
    13561 aagggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaataa
//
LOCUS       DAZUKT010000077        13478 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000077 DAZUKT010000000
VERSION     DAZUKT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.079"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..296
                     /locus_tag="ACG816_002272"
     CDS             129..296
                     /locus_tag="ACG816_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4921123.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(358..2169)
                     /locus_tag="ACG816_002273"
     CDS             complement(358..2169)
                     /locus_tag="ACG816_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4921124.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2166..2531)
                     /locus_tag="ACG816_002274"
     CDS             complement(2166..2531)
                     /locus_tag="ACG816_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4921125.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2694..3089)
                     /locus_tag="ACG816_002275"
     CDS             complement(2694..3089)
                     /locus_tag="ACG816_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4921126.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3107..4069)
                     /locus_tag="ACG816_002276"
     CDS             complement(3107..4069)
                     /locus_tag="ACG816_002276"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4921127.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4069..4281)
                     /locus_tag="ACG816_002277"
     CDS             complement(4069..4281)
                     /locus_tag="ACG816_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4921128.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4302..5000)
                     /locus_tag="ACG816_002278"
     CDS             complement(4302..5000)
                     /locus_tag="ACG816_002278"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4921129.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5020..5562)
                     /locus_tag="ACG816_002279"
     CDS             complement(5020..5562)
                     /locus_tag="ACG816_002279"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4921130.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5694..6698)
                     /locus_tag="ACG816_002280"
     CDS             complement(5694..6698)
                     /locus_tag="ACG816_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4921131.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6695..7684)
                     /locus_tag="ACG816_002281"
     CDS             complement(6695..7684)
                     /locus_tag="ACG816_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4921132.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7681..8487)
                     /locus_tag="ACG816_002282"
     CDS             complement(7681..8487)
                     /locus_tag="ACG816_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921133.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8653..9609
                     /locus_tag="ACG816_002283"
     CDS             8653..9609
                     /locus_tag="ACG816_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4921134.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9686..10204)
                     /locus_tag="ACG816_002284"
     CDS             complement(9686..10204)
                     /locus_tag="ACG816_002284"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4921135.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10292..10441)
                     /gene="rpmG"
                     /locus_tag="ACG816_002285"
     CDS             complement(10292..10441)
                     /gene="rpmG"
                     /locus_tag="ACG816_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4921136.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10669..11115
                     /locus_tag="ACG816_002286"
     CDS             10669..11115
                     /locus_tag="ACG816_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4921137.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11310..13205)
                     /gene="asnB"
                     /locus_tag="ACG816_002287"
     CDS             complement(11310..13205)
                     /gene="asnB"
                     /locus_tag="ACG816_002287"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4921138.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4206 a         2846 c         2293 g         4133 t
ORIGIN      
        1 ttataataaa acatttagta aacattttca agggtttttt gcaaaggttt acaaacaaac
       61 gtaaaactag cctttttttc tcttttgttc cataatagat ataacaaata ataaatgagg
      121 tggattcaat gaaaaaattt gtatctggaa tattagtagg aagtctggca acagccgctg
      181 ccgttgctgg tttggttgct tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag
      241 aagcaatgat cgaagaaaac cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat
      301 tatttaaaaa tacgcatgaa gactttctaa aaaacagata agtcatcatg cgtattttta
      361 tttgtcagtc aatataagtt gtgctatttt atctgcctgt ctcagattcc atggatctgt
      421 ataaggtcta gctactgctg cagttagcca atccacacct tcaacaggta ttccatagca
      481 ataaaagtca gactggattt cccctgaagc atcaatcacg cgattcgtac ctcgctcaac
      541 atctatagct tctgaatgat aacttcctgt attatctgaa aatgaaaaag ggcgaacaag
      601 tttgttttcc ttcaaatttt ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg
      661 caatcgagct tcaatcaaat agcgactgct gatttcttta tcttgtagag aaataacata
      721 gcggttgtgc ttttgctgaa tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc
      781 tatcagtgct gctaattctt tcgtccgaat gattggcgga ccgatcgtta aaaagctatt
      841 tagatgagta aaccatcccc atagtatttc tttgtattcc ttagcagaaa aaacgtgcca
      901 ttcaatagcc tgccggattg gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa
      961 tgctcctgtg caattgcctt tttccgcttc ttttatctgc ccttcaataa atattcggct
     1021 agcttctgaa aagggctcat tctgataggc aagaggagtt tctaaatacg accaatccca
     1081 tctataaggg attagtgctg gatgctttcc gagacactcc tctgaagaga gggagagaaa
     1141 atcatgctca aaatttttct ttggaatcgg caagtgatat tcttcgatga ttttcttgta
     1201 ataaaacaac tcaacgtctt ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc
     1261 cgactgatgc cattgctcca ggttttcttt tgttagtaac cgtggccatg ccattgctcc
     1321 gccttgcttt tgatttcttc ctcgagggta ataagggagc ccccttcttg atccactgta
     1381 tacaatcggt tccttgcctg atggatgata gaccagtttt cccttttcgt tctcgaagcg
     1441 ccctcctctt gatactgtaa acaatccgac ataatcaaaa aaacttaagc ctagacctct
     1501 taaaaagaca ttttctttag gcggtatctt cgaaagatct acatctgcag gattagaagg
     1561 aagctgatac agtaaaacat ttttctttgc gtagtctacc cacactcttt cttcttctgt
     1621 ctcatcattt tcccagtgac cgcttgctaa aacaagctga tcgatagaat agctttgctg
     1681 atctgtctgt aaaagataac catcttttct tttgcttact ttttttacta aagaataaac
     1741 aaactgaata gaagctggaa attcttcttg caactttgag aaaaaccagc gttgatagag
     1801 tccgtataaa catcgagtag attgctgatc tttttccaga cgattgattt cttctataat
     1861 tagttgctta tttttggtta ccgtctgttc gatataatct gtagcaggtc ctttactcca
     1921 ttgaaacaag ttgggtccct gtgttaccaa tcctccactg gacaatgttt catctgtaaa
     1981 aagcgtcact tgttgagaaa cagaattcat taataactcc ggtggctgat tcaaccgcca
     2041 aactcgacct ccaggaccat tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg
     2101 attattatgg agaatccttt ctgctacact taatcctcta ggtccggcac caatgatccc
     2161 tattttcatt tgcatgcctc catttcgata aattttttca ataaaacagc atggttctga
     2221 tgttcatttt ttgcaccata aacaaaagta atgactggat gttctttcat aagttgactg
     2281 agtttaaaaa tagcttgctt cttttcttct tcgaaaatca attcttgctg gtatttcttt
     2341 gcaaatgaag caaatcgttc tggtacatgg tcaaaagttt tgcgtgcttc attactaggt
     2401 gcaatttctt tcaaccacag atctaactgc gctttttctt ttgaaattcc tcgtggccag
     2461 aatctatcca cgagtattct atagccatcc tctttttcag catcaaggta aacacgtttt
     2521 aatttgatca tagttgtttc cttctttcta ccttttcgct tagcttatat atgtagataa
     2581 tagcacaaaa taaaaaaaat gaacggcaca actatccatt gcgccgttca tgtgatcttg
     2641 gtcaaaatct aatgattgat tcggttttta aagctgtcat acaatcatct gttttatact
     2701 tttttacttt tgatttttcc agaccatccg tcatatccac ccttaagtaa ataaatctct
     2761 tgataacctg ctttacgtaa tttatttgct gtacggatgc tcaaactttt cttttgatcg
     2821 taaatataga caggctggtc ttttcgaata gatcctaatg aatctttcaa aacagtgtaa
     2881 gggatgtttc ttgcacctaa aatatgccca gcatcaaaca catctttttc gcgaacatcg
     2941 attacttgtg cttttctcat tccttctcga aattcttctt ctgtcaaagt cgttgctgaa
     3001 cgtttggcca ttactcgtag atagacttcc caaaggatga ttgcaagcac aatcaatagc
     3061 aaaatgatat taattaccca taaaaccatg taatgcctcc gtttttttaa gcaaatttca
     3121 aggctaatga ggcagcacca atgacaccag cttcattacc aagctctgct aatttgattt
     3181 ttgttgattc tcttacttgc gggaaagtaa attctttaaa atatttctct acacggctac
     3241 gtaaaaattc tcctgctgcc gatactcctc cacctaacac gatacttgac ggatttaatg
     3301 tattccccaa gtttccgcaa gctaaaccta agtagaagca tacgcgatca acaaccatca
     3361 atgcaaacgg atcgtcttct tgagccattt caaagatatc tttacttgtt acttcttctc
     3421 cgttgtctag cattgctttc agttttgaat cccctgcgta ttcttctgcc aagaaacgac
     3481 ctaggcgtac cacacctgta gcgctagata cagtttctaa acatccgcgt ttaccgcaag
     3541 tacattcgaa cccttcagga tccacagtga tatgtccgat ttctcctcca gctccgctca
     3601 cgccgtgaat caaatttccg ccagcaacga tacctccgcc aacacctgtt cctagagtaa
     3661 cgaatacaac gtctggatcg ttttctccag cacctttcca tcgttcacct aaagcagcaa
     3721 cattcgcgtc attatcgata gcgaatttga ttcctgttcc tgcctcgatc agttttttca
     3781 caaattgaac ttctgtccag tttaagttat acgcaccaat aaccgttcca gcttcgctgt
     3841 ccacgcttcc tggggtaccc atgccgattc cgataaagtc ttcggctttc atattatata
     3901 aattcaaacg atgattgatt gattcaatga tttcaggaac gatatgctta ccatcatcca
     3961 aaatattggt atcgatgctc catttttgct gaatctctcc ttctggtgtt aaaattgcaa
     4021 attttgctgt tgttccacct aaatcaatcc caataatctt cttgtccatt acaaatttcc
     4081 tttctttcgg ctttcttcaa ttcggtgctc tctttttaat atgcgccacg ctgtcatgta
     4141 tgtatcatga tcaatcaaac ggctatcata cagattacgc aattcgatag tcattaattc
     4201 aatgtcatag atgcgtgcac ccacatagat aaagatgcca aattttttga acagctgctg
     4261 tacatcatat aagctctcca tctttaagca cttccttcat attatacaag taaaccatat
     4321 ttttttaaac ccagtaaaat acatattaca aaaagaaata caaaaactag tgtagaaaga
     4381 atcctttggt gtatcgaata gcttgtctgt tgtttcggta gacccaaaac attccctaac
     4441 actaatccgc caattagccc accgacatgt ccccaaacat ctacagaggt atccagaaga
     4501 ccaaaaacaa atgtcaacac aataaataaa agaaaatgtc tgactagctg ctggatcgct
     4561 gggttatgtt taaaatgaaa ccctaagata aacaacgcac cgaacaaacc gaatatcgaa
     4621 gtacttgcac caccagacag aacattcgct tcgttaaaag caaagcttgc tgtatttccc
     4681 attatcccgc tgaacatata aatcaagaaa aaacgccaat ggccatagat tgcctcgatt
     4741 tgctgcccca taaaatacag tatcagtgag ttcattacga aatgcatcag tccaaaatgg
     4801 atgaagattg gtgtcactaa acgccaccat tcattgaagt gaacaagata aggaccatac
     4861 attccggcat aataagggat ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct
     4921 gtctgtatcc ctaaaaaagc ataggtccat aacggtcgtc ttaaccatgc acgcaatttt
     4981 atttgctgtt gataattcat gaaaacctcc catttcattt tagtcaataa acaattgttg
     5041 gatcttctga tcaaagctct ccggttgcca ttgttcattc aattgttcac gaaaaacaag
     5101 gctacatgtt tttttatgaa aattatttaa ataccggtcg taataaccac cgccaaaacc
     5161 gattcgataa ccggctctat tgtagataat acctggtaca atgaggagat cgatcatctc
     5221 cggcaaatag agcttctctg attgtggctc aagaacacca aaactcgaac gtacaagaac
     5281 ggactgttca tccatttcat aaaacgccat ctgacgtttc ggcaaagttt taggtacaac
     5341 tattttcttt tgttcttttt ctgctcgttg gataaccggc tgtgtatcca gttcaaattc
     5401 attcgataaa gtcgtcccta tcacttttgc tgacttccag tactcgctgg cgaatagctg
     5461 tttatagatt cgctgttctt tttccttttt cttgccactt ttggccagat ttttcagctt
     5521 ttcgattcct ttccggcgta atcttacttt caattggtcc aaaaactcac ccttccttct
     5581 ctcataaaat ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa taaatgcata
     5641 aatagaataa aaagaagacc tagaaatgat tggccttcct tttttaatga atttcaactg
     5701 ttcagcgcgt gtttttgtct gataaaaagt gcatacagaa aatatggcgc accaaaaagg
     5761 gcaatcaaaa ttcctgtagg tacttcccca cttggtagaa tcactctagc aaccatatct
     5821 gcactaagca gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca gttattttct
     5881 ttacgtacga ctagcttcgc agcatgagga gctagtagtc ctacaaaaga taaactacct
     5941 gtcaccgcta cactaccgct tgccaaagca acagctaaca acaaaaggat tttttgactt
     6001 ttagccacgt taaggcctaa tccttgcgcg cgttcttgac caaatgatag caatacaatc
     6061 ctctgtgcat aaagtcctcc tagcggaatt aagataaccg cccatggaag caatgcccaa
     6121 acataaggcc agctcgcacc ccagattgat cccgctaacc atgcattcac aaatccataa
     6181 ttttccttag ataattggat cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg
     6241 gcaataccgg acaataagat tcgattcata cccaaaaagc catttcgatc ataggccgca
     6301 agatagacac agcaagcagc taaaacacca cctagaaagg ctacaactag caatagaaac
     6361 ggcagcgaat agtgcgaaaa gaaacctaaa taaagcatca ctaaaaatcc tgctccggca
     6421 ttaatgccca atatactaga atctgccaat tcatttcttg ttactccttg caagatataa
     6481 ccagaaacgc caagacaagc tcctgctaat aaactgatca gtaatcgagg aactcgaaat
     6541 tctctaacaa tcagcgccaa agaagatgat tctttcccta tgaacgtggc aaaaagctga
     6601 tccaggtaaa taaaaggcgt accaatcatt aaactaccta tagacaggat gacaagtaca
     6661 aaaagcaaga aatagaacgt ccaatatatt tttttcaccg ttgttccctc cttacttgta
     6721 tcagtaaaaa cggaacgcca atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg
     6781 gattgatcat cctcgctgta aaatccgcca acataaccaa tccaccgcca actaaaaaag
     6841 agacaggtag aacataacga tagtccgttc caaccattcc gcgaacaact ggcggaacca
     6901 acagcccaat aaatgaaaca ggtccgacta acgaaacggc aatggctgct aacactgcaa
     6961 ctagacagaa aacaatcaac cgcgttcgtt taggatgact tccaagagct aatgcatgtg
     7021 tttcactcaa atgaatcaaa gatattgctt ttcctaatga aaaagaaacc aaaacagcaa
     7081 gtatatataa aggaaacaat actgctaatt gtttccatgt gatattggca gctccgccta
     7141 caaaccaaaa agcaagatcc tgctgcagat taaagaattg ggtaagtgct tggctgatag
     7201 cagtaaaaaa agaactaata gctacgccag ctaaaatcag ctgtaaagga ttcatgccca
     7261 ttttcgaaaa ctgcaaaaaa ccaaatacga gcaaagttac agcaaaagaa cctgccaatg
     7321 acattagaaa cacagaaaaa tcagaagctt taggccaaaa agcaaaacta agggccattg
     7381 ccaacgaagc acccgcgttg attcctagta agcctgaatc tgctaaagga tttcttgtca
     7441 ctccttgcat caatgcaccg ctcaatgcaa aacaactccc tacaagaaat gctcccgcca
     7501 cacgaggcaa acgaatattt cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa
     7561 acaacgcctg ccaaaaatct gctttttcca cagtcgctgc gccatttgaa agagcaacaa
     7621 aaaaaataat caaaagaaca aaacagacca aaagcaggag tctatttttc ttcatagtcc
     7681 tcataattga tcctcctgct cttttggcaa caaatcatag gttagaaaaa ctggtttatt
     7741 tgttcttgga agcgtgacaa tcgttgcatc aatctcaaat atttttttca tacactcttt
     7801 tgtgatcact tcttcaggtg taccttctgt gatcaactta ccgtttttca ttgctaataa
     7861 ataatctgaa aaacgtgaag catgattaat gtcatgaatc gacatcaaga tcgttgtttg
     7921 tttcatttga ttgatttcag tcaataactg taggatctct aattggtgtg ccggatctaa
     7981 aaatgttgtc ggttcatcca agatcaagat atctgtttcc tgagcaagcg ccatagcgat
     8041 ccagacacgc tgggtttgtc caccagacag cgttgcgata tcccgcatac gaaatccagc
     8101 tagacctgta gcatccaacg cccactcaat tgcttcatga tcttgcgtgt ccatgccact
     8161 gaacagtttt tgataagggt atcgtccgta agatactact tcttctactg tcatacctgt
     8221 agggtgttca ctagtttgtg ctaataatgc aatttttttt gcaagttctt ttgaactgta
     8281 tgtagagata tcagagttat tgacaatgat ctttcctgat ttcacaggta gtatacgggt
     8341 aaaacttttc aataaagtag attttccact tccatttggc ccaatcaaac tagtgatttt
     8401 cccttcagga atcgtaacag acatgttttc aatgattttt ttgttttcgt agccggctgc
     8461 aatgtcagct gctcttaatc tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa
     8521 ttatgacgct gtttcaacat ttcgtcaagc gctactttca tatctatagc caaatgaagc
     8581 ggttgacagc gttcgaaata tcaggaataa taaccaatga gaaagattct caattatgtt
     8641 aggaggaaag aaatgaaatt aaaaacaaaa gtattcacat tatctagcat attgctgctg
     8701 gctggagtac tgagcgcttg ctccccctct tcagataaag aaacagctac aactgccaat
     8761 tcgacgactt catccaatag tactcataca gtcacagata cacttggaca taaagtagag
     8821 attccaaacg cgcccaaacg gatcatcggc agttacttag aagactatct gattgcacta
     8881 ggggaaaaac ctatcgccca gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa
     8941 gatgatttaa aggatgtccc aacgatttct tatgaccttc cttatgagaa agtgttaagc
     9001 tttgagcctg atttgctgtt gatttcttct tccgctactg tcgaaggcgg gaaatacgaa
     9061 caatatagca aaatcgcgcc aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa
     9121 gaacaactaa aagatgtcgg caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt
     9181 tccgattatc aaaaatccat caagaaaact cgagaagaac tagcagataa aatcgagaac
     9241 aagacagctg cagtgttgtg ggtgaccaac aactcagcct ttatggtcag caaaaatcgg
     9301 tcaagtggaa gaatcgtata cgacgatctg gaatttggtg ttcctgatct agtggaagag
     9361 gtctccaaaa aagccacttc tgactggtct gctgtttcct ctgaaaagct agctgaatta
     9421 gatgctgact acatcatcct tgtgaacagt gacaaagatg ctgccatgtt caatgaacca
     9481 agctggaaga atctaaaagc agtgacagac aatcatgtac tgcaattcgg acctgaatct
     9541 agttggctat ataacggacc tattgccagt caggaaatgg tcaatgatat caaagaaaaa
     9601 ctaaattgat tttaactaaa aaaagcatct tcacgattag tcgtgagatg ctttttttct
     9661 tattaacaaa ctagacgcat agattttatt cttcttttga agcaacccta ttttttactc
     9721 tttcccattt ttgaaaacga tgggcatata gattttcttg atccgttatc ccctcatact
     9781 cctctataag ggcgaagtct gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg
     9841 tgctatcgat caatgttcgc cagatccgac cagtgtaagg aagaagttct tgaaaaatcc
     9901 gactaccacc cgaaacatag atatcttctg agatcgtttg actatacgac aaaagttcat
     9961 cgatcgaatg cattacttcc gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca
    10021 cgatgatatg gcgataagga agtgaaagat cccccattcc ttcataggtc tttcttccca
    10081 tcactaaaat tcgatccatt gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc
    10141 atggtaacaa accatttttg ccgatcagac catttttatc ttgtgcccac atggaaataa
    10201 acatattatc cgctccttat gattttagat gttttgagat aaaaaaagtc tcaattcttt
    10261 gaccagcaag gaattgagaa ctttaaaaac attattttgt ttcacggtgc aaagtaactt
    10321 tgcgttcgcg tgggcaatat ttttgttttt ccaaacgatc aggattgtta cgtttgtttt
    10381 tgcttgttag atagttacgt tctttacaag aagtacactc taaagtgatg tttacgcgca
    10441 tgtttttccc tcccattttt acaagaatcc tctaagatga ctctcagact tgattatctt
    10501 accatgattt tttctagatt gctagtgttt ttcacgattt tgttaagtaa aactacttga
    10561 cgataaatac aacggtttta cttctcaaac aaaatctttt tctttttgta taaataccct
    10621 tacacttgtt tatatagatt atgtttttta ataaaggggg atactaatat gccatggaac
    10681 atgaaggact ttcctgcatc aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc
    10741 gacatcgcaa atgcattatt agatgaaggg taccctgata accgtgcgat cccaattgcc
    10801 attgatcaag caaaaaaatg gtatgaagat gctagtgaat cagaaaaacg aactttcgaa
    10861 aaagaaaaaa atccaagcaa gacggatgaa cacgatacga atcctcgtgc tggaaaactg
    10921 cttgattcag atgtgatcgt agaatatgag gaagaacaat ggatcgtgaa atctaaaggg
    10981 gcaaaaaagg caagcaacca ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa
    11041 gtagcacaaa acaaagaatc aactttgttc atttataaaa aagatgggac aaaagaaaaa
    11101 gaaatcgatt attgaacata tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt
    11161 atgttcgaaa caatggtgtt cctctgccaa ctccgaacca taagtcataa tatctataaa
    11221 acgggaagag ctgttttttg aaattgtttc ttatcactca gtgttaaacg gtaggagaaa
    11281 acctcttttt tattgatatt tgattttcat caaacagtca catagtcgat tgcttctgct
    11341 tgcggaatcg tttcatctac aaagtagact ttataccatg tcaagaaact gaagattgtc
    11401 cagatttttc tttgctcttc tttacgtcct tcgtgatggt catctagcaa cttcatgatt
    11461 ttttcttgat caaaaaattc ttttgcaaaa tctgcttcga ataatgcttt gacttgttcg
    11521 tagccgtgtt cttctttcaa ccaagctttg attggcacag ggaatcctaa tttcacacga
    11581 ttcgaccatt cttccggaag atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg
    11641 ttttgattca ataaaaactt acttggtatt tgttgagcca atttcatgac ttcgatatcc
    11701 aataaaggta ctcggacttc caaagaactt gccatagaaa gtttatctgc ctttaacaag
    11761 atatcttttg gcatccattg atgaagatcg acatattgca tcttattgac ttcatctttg
    11821 attccttctg ttttctcgta atgaacagtc atgatttcat ccacagatgg tgctgtttga
    11881 tattttggtg ttaacacttc tgatgcttct gattcttcga acactcgagc atgaccgata
    11941 aaatattctt ttgcaggagc aagtgattca tataaatgga tccgtccatg gaagtttttc
    12001 atttttccga ttttacgtcc taaattatag cgtgttcctt ttggtaattt tttcaatcct
    12061 tgagcaatca cacggatgaa ttttgaattc gtatggaatc catacgtttg gtatcccgca
    12121 aacagttcat ccgccccttc tccagattgg actacccgca cactttgtga agcaagattt
    12181 gctaaaaagt acaaaggtac gcaagaagga tttgaatcag gttcatctaa gtgatactgg
    12241 atcaaaggga atgctttgaa cgacatgtct ccgtcgatca ctgctgctgt attatctaag
    12301 tccaatagtt cggttagttt tctggcttca atcgcttcat tataggtttt atcatcaaac
    12361 ccgattgaga aagaatgatc tggtttcaat acagaggtta cataactgga atcaaccccg
    12421 ctggataaga aagaaccaac ttctacatca ctgaccgtat gtgcttcaat agaagcttga
    12481 acggtttcgt cgattttttc gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa
    12541 tctgcatccc aatattgctg aatgtctatt tttccatctt tatacgtata ataatggcct
    12601 tcaggcaaac gatagacatc tttgaagaat gtttcgccgt ttaatggaga atattggaaa
    12661 gtcatataag gtttcaacgc ttctttgttc aattctttgt taaaatcagg atgaggcaga
    12721 aaacttttga tttctgaacc aaacataaat gttccgttca tttctgcata ataatatggt
    12781 ttgatcccaa agtggtctct tgcaccgaat aactcattct tttcattatc ccaaatagca
    12841 aaagcaaaca ttccgcggat cttttgcaat aattctgttc cccattcttc gtaaccatgc
    12901 aaaaggactt ctgtatctgc atgtgtttga aaaacgtggc cagctgcaat caattcttct
    12961 ctcaaaggct ggtaattata gatttcgcca ttaaaaataa tgatctttgt ccgatcttca
    13021 ttgtaaatag gctgagtacc gccctctaag tcaataatac ttaatcttct gaatcctaaa
    13081 gcaacatgtt tatcgatata ttctcctgaa ctattaggtc cacggtgaac gattcgatcc
    13141 atcattgtat taataattgt ttttttgttg tccttgtcgt taacaaaacc tacaataccg
    13201 cacatttaac tttcacatcc taatataaaa ttactgttta tttcattcat atgcatctta
    13261 tcatagaata aatagcggac gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa
    13321 agatgaaagt ttctgttttt acagaaaata aagtcccaat atctaattta ccatgttttc
    13381 ttttttgagg ctaccttatt ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa
    13441 aaaaaccagc aaaaaaagac acgacctaaa gatcgtgt
//
LOCUS       DAZUKT010000078        13413 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000078 DAZUKT010000000
VERSION     DAZUKT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.080"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..352)
                     /locus_tag="ACG816_002288"
     CDS             complement(<1..352)
                     /locus_tag="ACG816_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4921139.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFT"
     gene            complement(600..1436)
                     /locus_tag="ACG816_002289"
     CDS             complement(600..1436)
                     /locus_tag="ACG816_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4921140.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1447..2376)
                     /locus_tag="ACG816_002290"
     CDS             complement(1447..2376)
                     /locus_tag="ACG816_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4921141.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2568..2921
                     /locus_tag="ACG816_002291"
     CDS             2568..2921
                     /locus_tag="ACG816_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4921142.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2995..3651)
                     /locus_tag="ACG816_002292"
     CDS             complement(2995..3651)
                     /locus_tag="ACG816_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4921143.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3877..5811
                     /locus_tag="ACG816_002293"
     CDS             3877..5811
                     /locus_tag="ACG816_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4921144.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5897..7120)
                     /locus_tag="ACG816_002294"
     CDS             complement(5897..7120)
                     /locus_tag="ACG816_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4921145.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7114..8985)
                     /locus_tag="ACG816_002295"
     CDS             complement(7114..8985)
                     /locus_tag="ACG816_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4921146.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9006..10124)
                     /locus_tag="ACG816_002296"
     CDS             complement(9006..10124)
                     /locus_tag="ACG816_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4921147.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10207..11040)
                     /locus_tag="ACG816_002297"
     CDS             complement(10207..11040)
                     /locus_tag="ACG816_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4921148.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11033..11950)
                     /locus_tag="ACG816_002298"
     CDS             complement(11033..11950)
                     /locus_tag="ACG816_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4921149.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12177..13301)
                     /locus_tag="ACG816_002299"
     CDS             complement(12177..13301)
                     /locus_tag="ACG816_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4921150.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4204 a         2685 c         2204 g         4320 t
ORIGIN      
        1 aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg
       61 ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca
      121 tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat
      181 ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct
      241 gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta
      301 gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta
      361 cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag
      421 gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac
      481 tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc
      541 cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt
      601 taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca
      661 actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct
      721 attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca
      781 aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta
      841 ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat
      901 tcgctagggt aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg
      961 atagtttgct tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat
     1021 ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct
     1081 actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga
     1141 ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt
     1201 ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct
     1261 gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc
     1321 attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa
     1381 acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt
     1441 tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga
     1501 taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat
     1561 tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga
     1621 tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat
     1681 gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt
     1741 ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc
     1801 gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt
     1861 cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt
     1921 gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag
     1981 ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc
     2041 gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa
     2101 cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca
     2161 ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt
     2221 ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat
     2281 aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact
     2341 catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag
     2401 ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt
     2461 tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa
     2521 attaaggtct catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca
     2581 ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt
     2641 cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac
     2701 attatgatat tccggcactt catttggatg catttgaaac accagaagta gctagtaaat
     2761 ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc
     2821 aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag
     2881 atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa
     2941 aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc
     3001 tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa
     3061 ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc
     3121 ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc
     3181 tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga
     3241 catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt
     3301 cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa
     3361 agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa
     3421 tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa
     3481 ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact
     3541 taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata
     3601 atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc
     3661 tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa
     3721 atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc
     3781 tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta
     3841 tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca agccaatcag
     3901 attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca
     3961 ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc
     4021 attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc
     4081 ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg
     4141 aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt
     4201 agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg
     4261 caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg
     4321 catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga
     4381 cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta
     4441 gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa
     4501 agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc
     4561 aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa
     4621 tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa
     4681 acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca
     4741 aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa
     4801 ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc
     4861 ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat
     4921 ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg
     4981 acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg
     5041 acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg
     5101 atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct
     5161 ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc
     5221 ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt
     5281 ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg
     5341 atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc
     5401 gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat
     5461 tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag
     5521 ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa
     5581 aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc
     5641 gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac
     5701 gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa
     5761 ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac
     5821 caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat
     5881 tccatattta cctttttcac caataagaag accaattact agtcattctc actaaagctt
     5941 ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat
     6001 tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca
     6061 attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata
     6121 gacctgattc tattaattct aataatccac aaaccatgat caccatacta gatgtatctt
     6181 tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca
     6241 accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca
     6301 aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg
     6361 aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca
     6421 tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa
     6481 tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca
     6541 ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag
     6601 gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg
     6661 cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg
     6721 ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc
     6781 tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct
     6841 tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc
     6901 ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga
     6961 tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt
     7021 tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa
     7081 acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt
     7141 gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt
     7201 ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg
     7261 ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa
     7321 gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa
     7381 acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat
     7441 gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata
     7501 ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca
     7561 agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca
     7621 tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt
     7681 cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc
     7741 catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc
     7801 aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt
     7861 caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt
     7921 agttccttca cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg
     7981 tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg
     8041 ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa
     8101 tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa
     8161 tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg
     8221 ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata
     8281 gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc
     8341 tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg
     8401 acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa
     8461 tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac
     8521 aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa
     8581 ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg
     8641 atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat
     8701 ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa
     8761 tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga
     8821 gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat
     8881 ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc
     8941 aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct
     9001 ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa
     9061 taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg
     9121 cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg
     9181 agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct
     9241 aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt
     9301 acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact
     9361 gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg
     9421 aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc
     9481 ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt
     9541 gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta
     9601 gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata
     9661 ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa
     9721 atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg
     9781 ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga
     9841 tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc
     9901 tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa
     9961 ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa
    10021 gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct
    10081 acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc
    10141 ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta
    10201 ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac
    10261 ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt
    10321 aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt
    10381 atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat
    10441 aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat
    10501 cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact
    10561 gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc
    10621 tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt
    10681 aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat tccccataaa
    10741 acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact
    10801 gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat
    10861 ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat
    10921 ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa
    10981 ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat
    11041 ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg
    11101 tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat
    11161 tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg
    11221 ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg
    11281 gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt
    11341 cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt
    11401 tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat
    11461 ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag
    11521 tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa
    11581 tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag
    11641 caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat
    11701 tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa
    11761 caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa
    11821 tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt
    11881 tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag
    11941 attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac
    12001 ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa
    12061 tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca
    12121 gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg tcctccttag
    12181 tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg
    12241 cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt
    12301 tgtgccactt gtggattttt tgcatcttta gggacataaa aacttgatcc tcctgatact
    12361 gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca
    12421 gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt
    12481 gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag
    12541 tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata
    12601 tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct
    12661 tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt
    12721 gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca
    12781 aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc
    12841 gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca
    12901 tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc
    12961 acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc
    13021 aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag
    13081 tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc
    13141 agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg
    13201 atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta
    13261 atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt
    13321 tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt aacacaaata
    13381 tttatataaa aaaatatttt acttgtcgtt ttg
//
LOCUS       DAZUKT010000079        13375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000079 DAZUKT010000000
VERSION     DAZUKT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.081"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..576)
                     /locus_tag="ACG816_002300"
     CDS             complement(<1..576)
                     /locus_tag="ACG816_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921151.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGF"
     gene            complement(587..1255)
                     /locus_tag="ACG816_002301"
     CDS             complement(587..1255)
                     /locus_tag="ACG816_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS4921152.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1386..1970)
                     /locus_tag="ACG816_002302"
     CDS             complement(1386..1970)
                     /locus_tag="ACG816_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4921153.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2305..2535)
                     /locus_tag="ACG816_002303"
     CDS             complement(2305..2535)
                     /locus_tag="ACG816_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4921154.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2550..3500)
                     /locus_tag="ACG816_002304"
     CDS             complement(2550..3500)
                     /locus_tag="ACG816_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS4921155.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3497..4339)
                     /locus_tag="ACG816_002305"
     CDS             complement(3497..4339)
                     /locus_tag="ACG816_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS4921156.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4472..5197)
                     /locus_tag="ACG816_002306"
     CDS             complement(4472..5197)
                     /locus_tag="ACG816_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4921157.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5658..5972)
                     /locus_tag="ACG816_002307"
     CDS             complement(5658..5972)
                     /locus_tag="ACG816_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4921158.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(5988..7583)
                     /locus_tag="ACG816_002308"
     CDS             complement(5988..7583)
                     /locus_tag="ACG816_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS4921159.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7606..8496)
                     /gene="iolE"
                     /locus_tag="ACG816_002309"
     CDS             complement(7606..8496)
                     /gene="iolE"
                     /locus_tag="ACG816_002309"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFS4921160.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8513..9538)
                     /gene="iolG"
                     /locus_tag="ACG816_002310"
     CDS             complement(8513..9538)
                     /gene="iolG"
                     /locus_tag="ACG816_002310"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFS4921161.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9562..10569)
                     /locus_tag="ACG816_002311"
     CDS             complement(9562..10569)
                     /locus_tag="ACG816_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4921162.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10682..11824)
                     /locus_tag="ACG816_002312"
     CDS             complement(10682..11824)
                     /locus_tag="ACG816_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFS4921163.1"
                     /translation="MPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTGHLQKIK
                     ELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTAKVVAVL
                     AANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKMMMKDLV
                     FMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEAMKKILN
                     TLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPHGISNAM
                     LLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLSIPTIRD
                     YGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11918..13372)
                     /locus_tag="ACG816_002313"
     CDS             complement(11918..13372)
                     /locus_tag="ACG816_002313"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4921164.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         4001 a         2892 c         2193 g         4289 t
ORIGIN      
        1 aaatccttta ttttttaaat cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc
       61 caaatttgaa gacggttcat ccaatataat taatttagag tttagcgata aactacatcc
      121 aatagaaatt ttctgccgtt caccacttga tagcgaaaaa atgtttttat ctaatatatt
      181 tgaaagatct aataattcag ctaattcatt gattctttta tccatatgct cttttgtaaa
      241 tccaaagttt tccatcgaaa atactaactc actcgttgta tttgttgtaa agaattgtcc
      301 tctaggatct tgacttacgt agccaatatt ttttgatata ctttcttgtg ttaaggattg
      361 tagcgagtta ttttcaaata aaatttcccc aaccaatttt ccgtcataaa gtcttggaat
      421 aagtccattc attaacttta atagtgtgga tttcccactc ccagaagaac cagtaatcaa
      481 tgtaagacta ccttttttta ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc
      541 cttatatgaa aaagaaacat tgttaaattc aaacaattct ttcacctcaa atcaaaatac
      601 ttaagaaaaa aatagtaacg gaaaacagga tgatatagtc taataagctc atgtttgttt
      661 tatagtaaga acttcttttc acaggagatt ctatcccctt tgttattgca ttagcggtca
      721 tttcttcagc aatcatagtt gcacgtataa gaacgggaac taacagaaat tccaaatatt
      781 gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt taaagattct ttaatcattc
      841 ttatttcaga acgataagtc ggaaagaatc tgatcactac tgtaatactt aacattattt
      901 ttttcggtaa atgtattttt tctaaagagg ataataattc attgctagta tagacactaa
      961 ccaacaacca tgagatcatt acgaccggca acattcgaaa tacaatatat aaggatgtcc
     1021 caaaaaatat agtaaaaaac tctatcttga tagcatcaat aattttcatt actaaaaaca
     1081 gaactgaata aatcaagatg aaataaatac atgtctttat tttacgaaac atcaatacta
     1141 aaactgtagc aataatgaaa caccccactt gaaatatatt tccctttcct aaaaaaataa
     1201 tgaccggaaa aaaaatattt aaagtaatca aggttaacgg attgattttt gacactcttt
     1261 cacctccaaa aaataagatc gcactgttat cttaagctga taaaaatgca ccgttattca
     1321 gataacagtg cgatcttatc aaaaagcata actgagaaca attctcaatg caagcttttt
     1381 tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt
     1441 aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag
     1501 aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt
     1561 ccgatagtct ttaacttatc tagacaacag actgtctttt ttgtcacaaa tgatgtaatt
     1621 ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaaccaa atcgaatata
     1681 cgtttattct caaaaatata attaataaat ggatctaatt cattgaagtt ctctctccaa
     1741 cttttctcta tatctatatt ttctaagttt aagaggtatt tggtaaactc ttcattatac
     1801 atattaataa gcgcattaat atgcggctga ataatttctt caaataacgc ttccttagaa
     1861 gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg tcaagcacag ttgccttatc
     1921 ttagtttgct gaaatcctac tttttcaaaa tggcttagtc cactttctat taatttttgg
     1981 tgagttgtaa tataatctct ggtttctatg ggtacttcct cctaataaga tgaattaact
     2041 atattatctt acacaggact tgaagtttca atcgttaacg tctattactg aactcattcc
     2101 ttagagctaa actaatgaga aagagcaaaa taactgatac aaaaagcaat cctacaagaa
     2161 ttaatagact catctaaatc attcaccttt tctaaaataa tccgagaaag atttaaaaaa
     2221 attatatcca gctattagga taagtcctat cataatcact ccaatagcca ttttcttatc
     2281 ctccctaaaa ataaagcctt ttacttaaaa cctttttttg taatcgtcaa taaatttttt
     2341 ccctttatca gttaattgaa atcctatatc taaatagtcc tcatatctaa gtttagtcag
     2401 catctcactt acagatgctg gactaacact tagaactttt gctaaatcat tattttttaa
     2461 tttctttttt ctttttgata attcctgaat tgctagtaaa taaattagta tagtagttgt
     2521 tttaatttgt accataccta ccctccaaat tatttcgaaa ggccttcagc aattttatcc
     2581 aggttccatt tcatcatcgc atagtaacta tcaccatctt gtcctttttc tgcaattgaa
     2641 tcagtaaaaa tcgttgaata gataggaata ttggtatctt ttgatactgt tttcattggt
     2701 ctatcgtcca cactgctttc tacgaataag gagggaactt ttgttgtgcg taatttttca
     2761 actaagtgtt ttatttgatc tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa
     2821 gcagaaggca cattatacgc tttcgagaaa tatttaaagc atccttcact tgtaacaatc
     2881 atttttttat cattcggaat tgaagcaaat ttagatttag cttctttgtc tagagaatcc
     2941 aatttttcaa tatacttatc tagattttct ttatagaatt ttttattatc aggatctttt
     3001 tccgctaact gtttttcaat atttttagcg taaataatac cgttttctaa atttaaccaa
     3061 gcatggggat cttccttccc tttctcactc tgaccctcta agtaaataac atctatgcca
     3121 tcacttgctg caaaatagtc tttgttttcc tctttgttcg cattttttac taattttgta
     3181 aaccaagcat ttcctccagt ttccaagtta acaccgttat aaaaaatcaa atctgctttt
     3241 gaagtttttt gaacatcttc aggcaaaggt tcatattcgt ggggatcttt cccaataggt
     3301 acgatactgt gtaaatcaat tttatctttt gctatatttt cagtaatatc tgctaaaatc
     3361 gaattagtaa ctactacctt taatttttcg ttcgaagcca ctgtatcttt gtctttacta
     3421 tttgttgaac atgccccgaa tataaaaaga gcaatcaaaa tccccagact tcccagtact
     3481 gctttccatt ttctcatcat aactttctct ccttctttct taaaaatttt tgttttggtg
     3541 agattagaaa actaattaca aataagaatg ccgctgttaa aacaatactt gatcctgctg
     3601 ctatgttaaa actataacca atgaacaaac ctagcaatga tgagcacgct ccgactgtcg
     3661 ccgaaataaa tatcatagtt tttaaactgt ttgcatataa atatgctgta gcagctggtg
     3721 taattaacat tgcaacaatt aatatagttc ccacactttg catagctgtt actgctacta
     3781 aagttaatag aatcattaat agataatgat aaaaactgac attcatcccc attgcttttg
     3841 ccattaatgg atcaaaagaa gtgattaata attctttaaa aaatagaaca actgtaacca
     3901 acactaaaac agaaacgcca attgtaatcc acttatcaat atcttgtaca gctaaaatat
     3961 ttccaaataa gatatgaaat aaatccgttg agctatttgc aactcctata aggataactc
     4021 ctaatgctaa aaaagaactg aaagtaatac caattgctgt atctcctttt attacactat
     4081 tgctttttat aaaagtaata atgatagaag ctaatagacc aaatacaatc gcaccaataa
     4141 aaaaattaat acctaaaata taagaaattg ctactcctgg caaaacagca tgagaaatag
     4201 catcacccat aagagacatt cctcttaaaa taataaaaca acctactgtt ccagaaacaa
     4261 tccctataac tacagatgta attagagcat tctgtaaaaa gtgaaagtca tacagaccat
     4321 taataaaatt ttgaagcata ttattcccct tgtatattag agttagggtt ttaaccttcc
     4381 ggttttgatt ggataaccct atgaaccatc gttattttaa tttggtatta actcatttct
     4441 tttgagataa tatatttatt ggattgagag gctaagccgt tcccccctct ccaataaata
     4501 tagtatctcc ataggctttt tttaaattct cttcattaaa tacagattct acacttccgt
     4561 gagcaattag atttcgattc aaaagaataa tattatcaaa atacttcttt accttgctta
     4621 aatcatgatg cacaattagc actgtttttc cctgatgttt caattctttt aacgtatcca
     4681 taattattcg ttcactaact aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat
     4741 ccgcattttg agcaagacat cttgctaaaa gtacacgttg aaattgtcct ccagataatt
     4801 ctcctatttg atgattagaa tattttccca tatcgacctt ttccaaagcc tttgaaactc
     4861 tttgccattc tgcattttta attctttgaa aaactttcat tccggcatat gttcccatag
     4921 atacacattc ttttactttt attggaaaag taaaatcaat atcagctttt tgctctacat
     4981 atgcaatctt caccagtttt ttattgatat cttcattatt aatggagacc tctccctgat
     5041 gagggataat atttaacatt gctttaagca atgtagattt tccagcacca tttggaccaa
     5101 taatacctgt taaagcacct tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat
     5161 cgttataaca aacattaagc tttttcactt ctaacataac tttctccttc taaatgataa
     5221 tgattatcac gatcatatgg tatacaaaaa ttatatttta gtcaacccta aattttataa
     5281 tttagatttt tttctgttgt tgaagattag gatgtcaaaa ttatttgaaa ctactcgtat
     5341 agtaaatagt aagtaatttt caaaacacga agaaaaacaa aaagaaaaaa ggaaacgccg
     5401 aagcatttcc tttcagtgat ttaatgagaa caacacgatt tgacttagag tctcaaaaag
     5461 tcgaataggt acactcattt aagtgtctcc attcgacttt ttggggatga gctggctatt
     5521 gaggtccgct taccctattg aataaataca agattgggta tttctaataa gtccgccctt
     5581 catattgggg tgatcggggc gaacatgcca tcttttcata taccacctcc tcgacactca
     5641 ataatcaaag attctcgtta atcttcttcg atgcttgcaa tgatgttttc aatattcata
     5701 taacgaaaat cttcttgttt gatataaacg atctgagcgg tacatttttc ctttttgatg
     5761 atttctttta ctttttcctc agaacgtgcg acctgcggcg ttaaaagtaa aacgtcatag
     5821 tctttataat tcagtttttc ttcaacgttt cggatcgttg cataatctat ctcatacgat
     5881 agatttttgg ctctgatcgt ttcaagcatt tttgatacga gcagcgacgc actgattccg
     5941 gtctcgcaaa taatcaatag atccttatgc ataataccct cctgttttta gcctgcaaag
     6001 ccaattggtg aaaatgccac ataagttaaa ataacaatga ctaccaatgc tatagcagtc
     6061 accttggtgt atttccaagg ctcgatatcg acctttgtat cgaatgagtt caacacaaat
     6121 tctccatcag gtttcaattt ggaaaccacc atcaaaatga taatgtccag aacgaataat
     6181 gccgtcaaaa cataaaggaa atggatattt tggaaaagca tttttgatag tgcatacaag
     6241 acgatatgca cggcaatcgt cgtttttgcc ccaaaagcag ttgctttttt tgaataaaag
     6301 gcaaagagaa tgatcacgag cagcggcatg ttgtatagtc cattgaattc ttgaacgaac
     6361 tgatacaatc cagccggtgc aaaagaaatg aatggcgcta cgataatgga gataatcccc
     6421 acaacgaccg ttactgtttt accagcacga gcgatttgct tatcatttgc tttcttattg
     6481 ataagcggtt tataaaaatc cagtgaaaac aaggtcgatg tcgcgttcaa agctcccgca
     6541 aatgatgata agatcgcgcc aaagataaca gcggcgaaaa taccaaagat cattttaggc
     6601 aatacttcac tgactaaaaa aggatacgcg ttatctggat ttgacattaa actatcgcca
     6661 aaaatattgc gagcgacgat ccctggaaag acaaggaata acgcacctaa gattttgaag
     6721 acgccgacta ggaaagcgcc cttttgtgcc tcagctaaat tcttgccgga caatgctttt
     6781 tggacgatca tttgattggt acaccagaaa tacagattgt taaacagcat ccctgtaaat
     6841 agtgtcggcc atggaacaac agccgagtcg atcgatccca cagagttaag cagccatggc
     6901 gtgtttacca caacgtgttc gatcccgcca acaaagctgc cgtcaccaag cttcattaag
     6961 cctaaaattg ggaccagcag accacaagcc agcagaccga ctccataaat ggagtcacta
     7021 ttggcactta acgacaagcc gccgatcagc agatagagga tgccgacaat accaattgcc
     7081 atcgctacaa ttatgatcgc gacgatcggc tggacgccca gcagctcatc aatatggaag
     7141 atcttattga acaccagcga tccagaataa agaaccacgg gtaagaaaga agtcatatag
     7201 gtcacaataa ataagatcga aataattcgt ttggtaaccg tatcataacg aatctcgatg
     7261 aaatctgaga ttgtattgat gccgtattta aaatattttg gcaagaatat cacagccaaa
     7321 gctacgatcg caatcgtagc agttacttcc caggccatta cttccatccc tgcatgataa
     7381 ctttgaccat tttgtccgac tatttgttcg gtggacaggt tcgtcatgat gatcgtgccg
     7441 gcgattggca aagcagttaa gctccgccct cccatgaaga acccttctga tccagaagta
     7501 tcgacacttc tggatctaga ataggcaaat aaccatacac agatgacaat cactaggaaa
     7561 ctgattatag aaaaaagatt catcgtctag ctccttttca atagattaaa gaccggtgac
     7621 ctctcgaatg tagcttctgg ctttgactgc atattcaaat ggattggctt tagccgggtc
     7681 ctgttctgct tcaactacga tccagccttc atagtcgctg gcatggattg ctgtccagat
     7741 tggagtgaaa tcaatcatcc catcaccagg aaccgtgaag acgcctttct tcacgccgtt
     7801 taagaagctg tctttttcct tttctacttc ttcagccgtt tcttgacgaa tatctttgaa
     7861 atggatgtgt ttgatacgat cttgatgacg gttgtaaacc gcgattggat tttcaccaga
     7921 aaagaccaaa tgtcccgtat cgaacaataa tttaactttg tctggatcag tcatttccat
     7981 taaacgatcg atttcttccg tcgtttgaac gccggtaccc atgtgatgat ggtaaacgat
     8041 ctccatatcc ttttcgtggg ccaaatcccc taatttttct aatccttccg ccagtttttt
     8101 ccactcttca tcagtgaaaa ctggtttatc atcaaagact ggcgctgtca taatcccttg
     8161 gatactatga ccctgctcag ctactacgat gactttagcg cccatcgcat gtaagaaatc
     8221 ccgatgtgct ttgaaggctt cggcagtttc ttcaaaaggt ttagttgtca gaaacgcact
     8281 gaaccatgca ctggcaacct ttaagccgcg aatattcaaa tattgttcca acacctctgg
     8341 atcttttgga tatttgttgc caatctctgt tccggtgaac ccagcaaggg ccatttcact
     8401 gatacattgt tcaaaggtat tttcactacc aagctcgggc atatcatcat tggtccacgc
     8461 gattggtgcg atggctaatt gaatttgttt ggtcatattc tcacactcct gcttattttt
     8521 ttagttgtac taaactcttc gtatcgaatg attcttgaca agccaacgcg atcttagttg
     8581 attgcaatcc atcttctgca ttaaccgctg gttgacgttt ttcttgaata catgagacaa
     8641 attcttgggc ttcacgaatg aaggcttctc tgaatcgttc tgggaagttt tgtgaacatg
     8701 gacggatgat cccttgatcg ttcatgatcg tcacaagatt tttctcaggc gcttgcgcta
     8761 cccgcagcat tcctttggtt cccatgatct ctgtttcgac atgatagcca tgggctgcgt
     8821 ttctgccggc aactagtaaa gctattgttt tatcgctcag gctcatcatt gcggctccag
     8881 tttcaagctc accagcctga tccaattctg gatacgccgc atttttccca atcgcccaca
     8941 cttgatcgac ttcttggccg gtaaaccagc gaaccaaatc aatatcatgg atcgccatat
     9001 ctaagaacaa gccgccgctg tcgttattca tggcaaattt gacaaagctt tccaagcctt
     9061 cgctcggatc gatcccataa caacgaatca gtgtgatttc gccgatttcg ccatcttcta
     9121 ccattttctt cgcatactga tacgactcat catagcggcg catgaagcct aacatgaaga
     9181 cctgttcagg atgctcttcg atgacttttt gggtcttttt aatatcttca acttcaaggc
     9241 ctaatggttt ttcagtaaag acatgcttgc cattttccat cgccaagcgg acttggtcac
     9301 agtgaaagcc tgatggtgaa acaataaaga ctgcatcgat cacggaactt ttgaccattt
     9361 cttcatagct ggaaaaagta tgcttgacgc ctaatttctt ctgtgcgaat gctaattctg
     9421 tttcaactaa actgcacgca gccgtaagct cacaacaact gacacctgtt gctaaatttt
     9481 gtgcatgtac ttttcctaat cttcctaaac cgacgatgcc tactctaatg ttgttcatga
     9541 aatacctccg ttttctgaga attactcatt gtatagtgct ggtcgttcat ccatttgaat
     9601 cattactttt gtttttgttt cacgagactc atgacaagcc atcatggtga tcgctgctac
     9661 atacccatcc caagcggtag gaccgacaac cgcatcattt ttgatcgaat cgatccacag
     9721 cttgaattca taatcatagg catccaaaaa gcgggtctgc caatctggtg aaacaggtgt
     9781 gtaatttttt tcttccgatt taattcgtgt ataggctggg tccgtcattc ctacttctgc
     9841 cgtttcgaaa accacatcac atttaatatc gtagccaaag cggcaattca caaagatttc
     9901 caaatcaatc gttacaccgg attttgtttg aaagatcagc agctgcggat catgcaaatc
     9961 ggcatgggag ttgctggttt ttttcggcaa gatcatttga acagattcat aatcttcatt
    10021 tagcaaccag cgaagcacat caatctcatg aatcgcggtg ttgctgatcg ccatcggtgt
    10081 tgtgtagttt tcatctgcgc taggatttct atgggcacaa tgcatcatta atgcttctcc
    10141 gtattgcttc gattcgattg cttctttcag ttcaatgtag ccgcggtcat agcggcgcat
    10201 gaagccaacc tgaaccaatt tcttcccata agcaacttcc gcatcaacga tccggcggca
    10261 gcctgctgca tccatcgcta atggcttttc gcagaaaaca tatttcccat gcttgattgc
    10321 ttctaacaca tactgctcat gggtcgggtc ccaagaagtc acaacaacga catccacatc
    10381 ttcagaagcg atcaatgctt ctccggtttc aaagaattta gcatcgacac gtttggcaac
    10441 ctctttagca gcttttacat tgatatctga gactgctgtc acataagccc cttgtgtttt
    10501 ctgattgatc ctgtcgatat gctcgatgcc gattgcacct gttccgataa cgcctacttt
    10561 tactctcaat tacttatccc cctgttctgt tgtttaagca cacatagtat gcgcttacaa
    10621 aattattcct gtgtagcttt tattcttaaa aggtagagcc ctctctttgc tccgcctttt
    10681 actacccata agcttgtacg tataattcct taatttgact atgactaggc acctttttat
    10741 tattttgcgg acttccgctg gtcaacgcat ccgcggccat tttgtctaag gctgccataa
    10801 acgcttgctg atcaatacca taatctcgaa tcgttgggat cgacagctgc tcacagagct
    10861 cttggatttt tttaatcaga taatccgcag cctcttccag cgataaatct tcggctgccg
    10921 gataaataaa tctggcgatc gtcgctaact cagtctgaat agcatccttt gagaattcaa
    10981 aaaagaccgg caataacatc gcatttgata tcccatgagg aatgtgaaaa agtgccccca
    11041 ccggtcgact cattccatga atcaatgtga ctgacgcatt cgaaaaggca atccccgcct
    11101 ccgttgaagc taagctcatt tgttctctgg cttcagtgtc gtcgctattt tcatatgcct
    11161 gagaaagcgt gttcaaaatt ttcttcatcg cttccagtga aaatagttgc gttacttcat
    11221 tggctttgac tgaaacataa gattccaaag cgtgacatag cgcatcgact cccgttgccg
    11281 cggttacttt tggtggtaca gttttcgtaa aatcagagtc aacgattgag atctgtggca
    11341 tgaaaacgag atccttcatc atcattttga ctgctgtttt tgtattggta ataactgcca
    11401 catcggttac ttccgaacca gttccagcag ttgttgacac tgcaatcagc ggaatgcccg
    11461 cttgcagctg atcaaaattt cttgcatgaa tcgcttcgag acttaattga ttagcagcca
    11521 aaacggctac caccttggct gtatccagac agctgccgcc accaaaagca atgatatgat
    11581 cacattgatg cttctgaaaa agcttcagcg catcataaac attttcttgc gtcggttcgg
    11641 cttgaatgtc tgtatactcc gctaattgat catctgtttt tcccaacagc tccttgatct
    11701 tttgaagatg cccggtctgt tccataaccg tatcactaat gattaacggc ttgttgccta
    11761 gttgttctga ttcttccata agaaattgga ttgttccgcc gccaattctt aaattggcag
    11821 gcattttcag ttgaatattc acagaaaaaa cctctctctg gcttatagcc tataacagaa
    11881 tcctcgaagc tctttaacag agcttcgaag caaatcttta aatctcactt tgcgcataac
    11941 gtgctgtaac aactttccga tgggtataaa actcaacact gtctttgcca ttggcatgca
    12001 aatcaccaaa gaaagagtgc ttccagcctg aaaaggcaaa ccaagccatc ggtgcaggca
    12061 ccccaagatt gacccccagc atcccagcat caacattttc tctgaagtag cgaatcgctg
    12121 atgcgttgtt ggtatacaga cacgaaccat tcgcaaattc agattcattc gtcagctcaa
    12181 cggctgccat caaatctttt acccgcacga ttgatgcgac cggggcaaaa atctcatcct
    12241 gccagatctt catatccatc gtgacattat caaaaatcgt tggtccaaca aaatagcctt
    12301 tttctggaat gttttcgcga ccatctagta ccagtttcgc tccttcatcg accccttttt
    12361 ggatatagtc aaaggtccgc tgctgtgcat cttttcgaat cactggtcct aaaaagaccc
    12421 cttcgtccaa gccattgccg atcttgatcg cttttgccgc agccttaaat ttttcaacaa
    12481 actgatccgc gatttcctct tccaaaacaa tgacggaggc tgccatgcag cgttcaccag
    12541 cagaaccaaa agctccgcca ataaggtcct tgactgcccg atcaatattg gcatctttca
    12601 gcacaacaac atgatttttc gctccagtca acgcttgtac ccgttttaga ttttttgttc
    12661 cttcacggta aacgtattca ccgacatttt ttgatcctac aaagctgatg ccttttacca
    12721 gtggatcttg cagcaatgtg ttgacaacat cgtgggcacc atagacaaca ttaaaaacac
    12781 cgctaggaaa gcctgctttt tcaaccagct caacaatttt ctccatcaac aatggtgttt
    12841 tttctgaagg cttcagcacc accgtatttc ctgccgccag agccatcggg aagatccaga
    12901 agggcaccat cattggaaaa ttaaacggtg tgatccctcc gattactccg attgggtaac
    12961 gataattcgt gatctcaata tcagtcgccg ctgttgataa agaatctccc atcattaaat
    13021 tggtgattcc cgctgcgtgc tccacatttt cgattcctcg ttgaacttct cccagtgact
    13081 cggacaatga tttcccattt tccagcgtga tcaaacgcgc caattcactt ttatgatctg
    13141 ttaaaagctg atgcagcttg aacagataac gtccgcgaac aggaacagga acttttttcc
    13201 aagtttgaaa cgcagcatcg gcagctttaa ctgcttcggc tgtctctgct ttgctagaca
    13261 acggtacatt cgccagcgct tcttctgtag ccggattcat tacttcttca aactctgttg
    13321 agcgactagc gacccaacga ccatcaatat aatttcttaa aaatcttgtc attta
//
LOCUS       DAZUKT010000080        12915 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000080 DAZUKT010000000
VERSION     DAZUKT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.082"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG816_002314"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG816_002314"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4921165.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACG816_002315"
     CDS             complement(1036..1956)
                     /locus_tag="ACG816_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4921166.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACG816_002316"
     CDS             2069..2821
                     /locus_tag="ACG816_002316"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4921167.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="ACG816_002317"
     CDS             3685..4218
                     /locus_tag="ACG816_002317"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4921168.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4708..5232)
                     /locus_tag="ACG816_002318"
     CDS             complement(4708..5232)
                     /locus_tag="ACG816_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4921169.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="ACG816_002319"
     CDS             complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="ACG816_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4921170.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5821..6801)
                     /gene="pta"
                     /locus_tag="ACG816_002320"
     CDS             complement(5821..6801)
                     /gene="pta"
                     /locus_tag="ACG816_002320"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4921171.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6954..7634)
                     /locus_tag="ACG816_002321"
     CDS             complement(6954..7634)
                     /locus_tag="ACG816_002321"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4921172.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7760..8629
                     /locus_tag="ACG816_002322"
     CDS             7760..8629
                     /locus_tag="ACG816_002322"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4921173.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8655..9170
                     /locus_tag="ACG816_002323"
     CDS             8655..9170
                     /locus_tag="ACG816_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4921174.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9217..9780)
                     /locus_tag="ACG816_002324"
     CDS             complement(9217..9780)
                     /locus_tag="ACG816_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4921175.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9835..10014)
                     /locus_tag="ACG816_002325"
     CDS             complement(9835..10014)
                     /locus_tag="ACG816_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921176.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10091..10600)
                     /locus_tag="ACG816_002326"
     CDS             complement(10091..10600)
                     /locus_tag="ACG816_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921177.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10696..11076)
                     /locus_tag="ACG816_002327"
     CDS             complement(10696..11076)
                     /locus_tag="ACG816_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4921178.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11406..12155
                     /gene="pnuC"
                     /locus_tag="ACG816_002328"
     CDS             11406..12155
                     /gene="pnuC"
                     /locus_tag="ACG816_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4921179.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12476..>12915)
                     /locus_tag="ACG816_002329"
     CDS             complement(12476..>12915)
                     /locus_tag="ACG816_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921180.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         3733 a         2612 c         2321 g         4249 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa
     4501 tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc
     4561 gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga
     4621 ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga
     4681 cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc
     4741 taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc
     4801 catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg
     4861 aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag
     4921 tcccattccc caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg
     4981 aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag
     5041 cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg
     5101 tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc
     5161 catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac
     5221 ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt
     5281 gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta
     5341 aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa
     5401 cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta
     5461 cccgataaat atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg
     5521 tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca
     5581 acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg
     5641 aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt
     5701 ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa
     5761 agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg
     5821 ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt
     5881 tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc
     5941 ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg
     6001 aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac
     6061 tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag
     6121 ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc
     6181 ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt
     6241 tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca
     6301 atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt
     6361 ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt
     6421 cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc
     6481 aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct
     6541 acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat
     6601 cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt
     6661 ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat
     6721 acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct
     6781 taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca
     6841 ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat
     6901 taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact
     6961 gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt
     7021 ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga
     7081 gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt
     7141 ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct
     7201 gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga
     7261 acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca
     7321 ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc
     7381 tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga
     7441 tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag
     7501 atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt
     7561 agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga
     7621 atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc
     7681 acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca
     7741 gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt
     7801 agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc
     7861 cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga
     7921 tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga
     7981 aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat
     8041 tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa
     8101 tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac
     8161 gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt
     8221 ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt
     8281 tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga
     8341 tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa
     8401 aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac
     8461 gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat
     8521 gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga
     8581 atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga
     8641 aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc
     8701 ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa
     8761 caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc
     8821 agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa
     8881 ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga
     8941 cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca
     9001 tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg
     9061 atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta
     9121 aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac
     9181 ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc
     9241 ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa
     9301 ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt
     9361 cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc
     9421 ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa
     9481 caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat
     9541 gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc
     9601 ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa
     9661 aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag
     9721 aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat
     9781 agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt
     9841 taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc
     9901 agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt
     9961 gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc
    10021 tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa
    10081 tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga
    10141 ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt
    10201 tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt
    10261 tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc
    10321 cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg
    10381 cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt
    10441 cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt
    10501 cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta
    10561 ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt
    10621 ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg
    10681 acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa
    10741 cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga
    10801 ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc
    10861 acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc
    10921 agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct
    10981 gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac
    11041 aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta
    11101 agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc
    11161 tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac
    11221 tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca
    11281 gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc
    11341 atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct
    11401 catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa
    11461 attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca
    11521 tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat
    11581 ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta
    11641 ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga
    11701 tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa
    11761 tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc
    11821 ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca
    11881 ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat
    11941 attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc
    12001 atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc
    12061 ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg
    12121 aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta
    12181 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct
    12241 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    12301 ttcttggagt taaacgcttc tgtcccagcc tctatttcct acgattagtc aagtaaaagg
    12361 tgcaagaaaa atagctcgta aacgaaagcg acaaaatcaa agcacaccaa ataaagcccc
    12421 gttcgtaaaa aattttcgat ctgggcaaag aagtggtata ctgtaagtgg tttagttaat
    12481 ggtccactga gaccgcgtac gcatagtcgt actctgtatg tgccctaacc atagcgtgca
    12541 tacatttcag aagcttgttc atacaagcaa ctgtggcaac cttatctttc ttagggatag
    12601 gttgcttttt taatttgtag taataatcga caatatgatt aggagctgcg cgttgctggc
    12661 gaatcatatt tcgaatggtg aagaaaataa ttttccgtgc tttgggatta ccacgcttat
    12721 tgatgcggtc ttggccagta tacttacctg aatgataccg tcggatgtcg attccgacaa
    12781 aggcattcaa ttgtttatgg gttttaaatc gtgtgatatc tcctaattca ccgagcaata
    12841 aagcggctgt gagtgcgcca atcccaggga acgtttggta caacaaaaag caaggagagc
    12901 cttcggcttt tttga
//
LOCUS       DAZUKT010000081        12740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000081 DAZUKT010000000
VERSION     DAZUKT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.083"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..542
                     /locus_tag="ACG816_002330"
     CDS             99..542
                     /locus_tag="ACG816_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4921181.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="ACG816_002331"
     CDS             complement(548..1732)
                     /locus_tag="ACG816_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4921182.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="ACG816_002332"
     CDS             2000..2698
                     /locus_tag="ACG816_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4921183.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="ACG816_002333"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="ACG816_002333"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4921184.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="ACG816_002334"
     CDS             complement(3508..4041)
                     /locus_tag="ACG816_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921185.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="ACG816_002335"
     CDS             complement(4338..6719)
                     /locus_tag="ACG816_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4921186.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="ACG816_002336"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="ACG816_002336"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4921187.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="ACG816_002337"
     CDS             7420..7959
                     /locus_tag="ACG816_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4921188.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="ACG816_002338"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="ACG816_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4921189.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="ACG816_002339"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG816_002339"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="ACG816_002340"
     CDS             9066..10232
                     /locus_tag="ACG816_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4921190.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="ACG816_002341"
     CDS             10278..11774
                     /locus_tag="ACG816_002341"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4921191.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="ACG816_002342"
     CDS             11855..12538
                     /locus_tag="ACG816_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4921192.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12547..>12740
                     /locus_tag="ACG816_002343"
     CDS             12547..>12740
                     /locus_tag="ACG816_002343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921193.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4273 a         2230 c         2630 g         3607 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    12661 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    12721 gcaggagaac accgtttatt
//
LOCUS       DAZUKT010000082        12431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000082 DAZUKT010000000
VERSION     DAZUKT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.084"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..189)
                     /locus_tag="ACG816_002344"
     CDS             complement(<1..189)
                     /locus_tag="ACG816_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921194.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(199..1014)
                     /locus_tag="ACG816_002345"
     CDS             complement(199..1014)
                     /locus_tag="ACG816_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4921195.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1276..1719)
                     /locus_tag="ACG816_002346"
     CDS             complement(1276..1719)
                     /locus_tag="ACG816_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS4921196.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1853..2191)
                     /locus_tag="ACG816_002347"
     CDS             complement(1853..2191)
                     /locus_tag="ACG816_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4921197.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2179..2556)
                     /locus_tag="ACG816_002348"
     CDS             complement(2179..2556)
                     /locus_tag="ACG816_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4921198.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2780..3973
                     /locus_tag="ACG816_002349"
     CDS             2780..3973
                     /locus_tag="ACG816_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4921199.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4139..4561)
                     /locus_tag="ACG816_002350"
     CDS             complement(4139..4561)
                     /locus_tag="ACG816_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921200.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4956..5150
                     /locus_tag="ACG816_002351"
     CDS             4956..5150
                     /locus_tag="ACG816_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921201.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5147..5593
                     /locus_tag="ACG816_002352"
     CDS             5147..5593
                     /locus_tag="ACG816_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921202.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5803..6975)
                     /locus_tag="ACG816_002353"
     CDS             complement(5803..6975)
                     /locus_tag="ACG816_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4921203.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7181..7996)
                     /locus_tag="ACG816_002354"
     CDS             complement(7181..7996)
                     /locus_tag="ACG816_002354"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4921204.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8146..8592)
                     /locus_tag="ACG816_002355"
     CDS             complement(8146..8592)
                     /locus_tag="ACG816_002355"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4921205.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8589..9104)
                     /locus_tag="ACG816_002356"
     CDS             complement(8589..9104)
                     /locus_tag="ACG816_002356"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS4921206.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9177..10091)
                     /locus_tag="ACG816_002357"
                     /pseudo
     CDS             complement(9177..10091)
                     /locus_tag="ACG816_002357"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10088..12415)
                     /locus_tag="ACG816_002358"
     CDS             complement(10088..12415)
                     /locus_tag="ACG816_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS4921207.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4216 t
ORIGIN      
        1 gcaacctgga ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc ttccatctat
       61 cgacaaaagg aacgcagctg ctatatacaa gatattgaca caccaaaaac aaaggctact
      121 tgccaatgat tgatacaacc aataaaagaa ttccatataa acatttttgt caaaacgctg
      181 ctctttcata atcacctcct attccttcat tcctgatgaa gcgatccctt ctacaaaata
      241 tttttgcatc gctaaaaata taaaagcaac aggaagaaca gaaacaactg aggcagccat
      301 gattaatgcg tactctgcat caaatagccc aataaacatt ttcaacccta actggattgt
      361 tttattttcc gtagaagaca aataaatgaa tggccccata taatcgttcc atgtattaac
      421 gaatgtgatg atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg
      481 atagattccc cactctgata agccatcgat ccgcgcactc tcacataaag aatctgggat
      541 actactgtag tattgcttca acaagaacac accaaaagca ttgaacgctt gtaatagcac
      601 caacgaccac aacgtatatg ttaaacccac ttttctcatc ataatgaact gcgggatcat
      661 ataagactgc caagggacag caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc
      721 tttaaaattt agtttagaaa acccgtaagc agcaaaacta gaagtaaaca actgaatcaa
      781 cgtgatcaca acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag gaatacgaga
      841 ccaaataacc agataattct ctaaatgcca agttttaggg atccattgaa tcggaatcgt
      901 gaacacttca ttatttgctt tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt
      961 caataaagct aatacaatca gcagaatcat acccaaccat tgaataaagg gcatgttttt
     1021 tgaacgaatt tttgcttttt ttctttcatt tcgaacacct ccaaagttat aatcttcatt
     1081 tctttgtaat gattacaatc cagaacaacg gttattatag ctgattcatt tccgttcttc
     1141 ttatgtttgt aagcgcttat ttaacaagtt atgtaaatct ctttattttt tttgatttaa
     1201 aaatgaaaaa atacaaaagc aagactgtca tacgtctcgc ttttgtattg aaatttcttc
     1261 gattagagat acttattact gttcaaaagc ttctttgaaa cggtgaatca attcattgac
     1321 tacaggtgat aaaatccgat tctttttcca gactagcatg cagttcgttt caatcattgg
     1381 gtcaagcgga acgaatgtca ctcgctttga aagatgactt acagcacctt ctatgactaa
     1441 agcagcacct acctgattct caattagcgc aaatacattg aaaatcaaat tgtacgttcc
     1501 tgtgatcagc aactgctcct tttttatgcc taaccagtca gcaaacattc gctgtatttc
     1561 tggacgcttt gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt
     1621 ttttgctaaa acagaatttt ctaaaatgag caatccccat ttttcctttc ttggtaacaa
     1681 aatcgtttcg tatttctctg tatctactgg atctagcaaa atcgctaaat ctaacaggct
     1741 tttttctagt cactccgtga tatcatcact tgttccgctg tggatatgaa attgaacgct
     1801 gggataatca cggatcaact tttctagtaa tagtgccaat gtattcgaat tatcagcttc
     1861 cacacagccg atcgtaaatc ggccgctgaa taattgtttt tttctgttct cgaattcttg
     1921 agtggtttga ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga aaataacctg
     1981 cttctgtcaa gatcattttg cggttttcgc gtacaaacag ttctgttcct agttcctctt
     2041 ctaactcctt cagttgtcgg cttaaggttg gttgtgtaag atgtagaact tctgcggctt
     2101 gagagattgt tccttcttct gcaattgtcc aaaaatattt tagtaagcgt aactccatag
     2161 cgatgctcct tgattttttt acttgataca ttccatttta tttctagata acacacgatc
     2221 atatttcttg atttttcctt gtaaacgttc caaacactct tgcatttcag caatttcttt
     2281 cgtcaaaagt tcactttctt caatcaaaag cttacgccgc tcaaatacag ttgcttctcc
     2341 ttcgctagat aggcgaatat attcaatcaa agactcgatc gataatccgg catttcgcat
     2401 acagatcgta aagtcgaccc attttaggtc ttcttctgta taatcacgga ttccgccttt
     2461 tgtccgatta actggtggaa tcaacccgat tcgttcataa tagcgcaatg tatcggccga
     2521 aatgcccttt tcttcactga cttgtttgat attcatctga tcactctttc tcttaatttc
     2581 ttgccacttt acttatgtat tttatttatt ctacaacttt aagtgtactc caagtcaata
     2641 ctcatttcta ttaaaactaa gctggattat ctatatcggt tgtttgtgta ttatttcaaa
     2701 taaaaaagtc ttgttttttt attctttttt ggatatactt ttaacaattt agggaaattt
     2761 taggaaaagg ggtataaaaa tgacaacgta tcagcgaaag gtattattat cgacatcggc
     2821 gggaattgct ttagagaata tggatattat gtttttagct ttttcattat cttcaatgat
     2881 tacttctttt catattagtg gtacgcaagc gggtttcatt tcaacgatca ctaatttggg
     2941 gatgctcatt ggaggtattt tttttggcat catagctgat aaatatggac gtgtgaaagt
     3001 attttctcaa acggtccttc tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa
     3061 catctacttg gtttatctct ttcgctttat cgcaggtgta ggagcgggag gagaatacgg
     3121 ggcgtgtatg tccttgattt ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc
     3181 ggttgctggt attggtgcgc aggtcggtgc ggctttggct gcgattttag ctgctgtgat
     3241 cattccaacg tttggttgga agatgttgta tgtcgtaggt gtactgccgg tgttgatggt
     3301 cttgttcatt cgtcgcggct tgactgaacc aaaggaattc aaatctgtac aatctgccga
     3361 aaagaaaacc aaacttagca gactgtttga atcaaaacat cttgcttggc aaacatgtgg
     3421 ccttagtatc atggtaactg tccaaattgc cggctatttt gggttaatga attggctgcc
     3481 ttcaattatg caaaatcgat tagggctttc ggtttctggg tcttcattgt ggatggtcag
     3541 tactatttta ggcatgtctt tggggatgat gacttttggc ataatcatgg ataaattggg
     3601 gcctaaacta gccttttctt tgtttttaat ttgttcatcc ttatctgtct atctacttgt
     3661 gttagcccat agccagtgga gtttggtagc agccgctgtg atagtcggct actttatcaa
     3721 tggcatgtat ggtggatatg gcgcagtgat tagtagtttg tatccaacag aaatccgcgc
     3781 gacggcgaat aatttcatta tgaaccttgg acgagctgtt ggtggttttt cttctatcgt
     3841 catcggtttc ttgatggatc attatagttt aactgccgta atcttgtttt tgagcggtat
     3901 ttacctagtc agtctgctgg tcttgacaac tcttactggt gttacagcat taagggaaag
     3961 cttgcagcat tgatagttga gtctgtaaat gacaagaaag aggtgtggca aaagcttgca
     4021 tctgcggtaa taagaccagc caaatcaaaa acatgagaca ctgtttccgt ttggctggtc
     4081 gtattacttg atgcagggca gctttttcaa ctgctaattt ccttctatct ggcggcgact
     4141 attctttgac gtccgtcatt tcaaagtact ttactaataa cttagcataa cgaaaaactg
     4201 tggatacact cgtatattga gcgtctgcat aatcttccaa atttttctca ggatgttgat
     4261 acatgaattc aatcaaatga taattatatg attttctgta gaaatgaaga taaataatgt
     4321 agattgggaa gttctcctgc ttttccatag aaaagcgccg attattctca ttcttcaaaa
     4381 tgaccttatc aggatatttt tggaacaatt cttctaaatc tgttagataa ttcaagatag
     4441 acttacttga taccttcaat tcgttttgta gttcttctat tgaacaccaa cgttctgttt
     4501 caagcagtaa atgaatcatt ttaattttct tttgagaaac ttttgttaaa aaagtctgca
     4561 tattttctcc tcctgtatct actttactat actacataca agaaaaaaaa cggatacaaa
     4621 tatcatattg tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttccta
     4681 agtattctat tgatatgaat catttaatcg tttattatat aaatgtcaat aatctaataa
     4741 taaaataacg tacatatttt attattgcat acttaataac agaactatag caaaaaatcc
     4801 cgtgtatcaa cacgggatta gattaagttt cctcaatcac tattcataac acaattatta
     4861 taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag
     4921 gaggaaattt agcgaaaaaa ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg
     4981 ggtgtggtgc tactacgtct actactcaaa gcacaagttc agatcaggct tccagcagca
     5041 gtaccacgat ctccgaaagt acggcagatt catcgaaaga acaagaacaa attgaaaaga
     5101 aaaaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga
     5161 atctgctttg tcacaagctg aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc
     5221 agctatccaa gcgctacctg aaaacaaaca agagttgaca agccgtcttg ctgcaattga
     5281 ttccacaatc aaagcaaaag aagcagaaga agaacggaaa aagcaagaag aaattgcacg
     5341 acaacaagca gaacaagcgc aacaagaagc aatcgcacag caacaagcag aacaggcgag
     5401 gcaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaatcag cagctgaaca
     5461 ggcctctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
     5521 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
     5581 cactttaagc taagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
     5641 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagatggc
     5701 gttccagata gtaaaccaaa aataacagtt tacacgtcca tttcgtcagc ttctctcttt
     5761 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     5821 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     5881 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     5941 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     6001 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     6061 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     6121 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     6181 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     6241 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
     6301 atgtgtattg accatataag gagtacaggt aagcaaagtc acgagatctc tgcctttttg
     6361 gatacccaaa gaatctactt catcaggtaa gaccacttgg attttttcta cttgatacgc
     6421 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
     6481 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
     6541 cccgccagtc ggataagaac tttcttccaa taatgacgtt cctttttgca aaaatatttc
     6601 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
     6661 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
     6721 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
     6781 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
     6841 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
     6901 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
     6961 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag gttgtgaaat
     7021 cagcgccttg acctgagtat tagcgaagca aacggcaaaa tagttcctcc tattttgccg
     7081 tttgttgagc gaataccaag agaccgacca cttgtcacaa cctttctccc catttgaatc
     7141 actatcgttt atttatcgta tgatttttgt ttccaatagt ttaagcttct gtttcttgat
     7201 ggcgtttttt gaaatagatc actgctcctc caactaaagc agtacctacc aagatgaata
     7261 cgatgatccc cataccacca gttgaaggca agattccttt aggttggtta tcgatcgttg
     7321 tttcatcaga agttgttgga tcatcatcta ctgtaaattc agtgtacgca gcttcattct
     7381 taggtaatgc gtagccttct ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca
     7441 gcccttcgat tgaaaatttc ccttctgaat cagaaatcaa tttcacttct tcccagtcag
     7501 aacctgtgac ttcatctgac caagcatatg ggtattctcc tgaggcaagg tttgctattt
     7561 ttttcattaa tttaacagtt ggatcttttg ctcttagaat agaaaattct gcatcggcta
     7621 atttttcttg cgtgttcaca tcttgtttga tgaagtggaa atcaccgtag ttttggacat
     7681 tttctacagt aatcgtgtta tccgtagttg tattttcccg attgactaca ccgtttggat
     7741 tcccaagatt ggcttcatct gcgccatgga tgataccggt tgtttgttgc aattttccgt
     7801 tttttactat aaaagtagtt tctttttgtg gattggttaa ttgataacca tctggtgctt
     7861 ttgtttcaac taaagtataa ttgccgtcta ttaatccatt gacactgatg gatccttcag
     7921 tatcagttgt gaatgtcaac gctgactcag caggatcata gagagcgaca ccagtgtttt
     7981 catcaaaacc ggtcacgaat tgattctctg aatttttgat ctggaattgc gcccctgcta
     8041 acttatctac ccctgaaaag ttcccagctt tctggatttt gatattgctt tctttgatga
     8101 catttttagc atataggtga atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa
     8161 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
     8221 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
     8281 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
     8341 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
     8401 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
     8461 attaaccgta acattttctg gtgtaggtga tacggctgca gaaacctctg cagttcctag
     8521 gccgagaacg aaaggaagca gcatgatagc agttcctaaa aacgtccata tttttgtttt
     8581 gatgtttttc attttatttc cctcctattg tttccttttt gttgtttttt ataaaaccaa
     8641 gctgtccctg ttgaaaggac tagcaataac cctaaaattg agaaacctcc aagcatctct
     8701 tcgcttgttt ttggaaagct tcctccattt ttttcatttg atggaccatt tgtctgcatc
     8761 tgaactggcg tttttccatt attcgtcgga tttgcttttg gttcgttgat gatttccaat
     8821 ggtataggat gaagcggatc ataactgcct ttttcaactt caaagagaat ggttgattga
     8881 ctcaatatat atcctttagg cggtttaatc tccactagtt cgtaacttcc aaaagacaat
     8941 ccatggacag aaaatgttcc atctttactg gaagtaaaaa tcgttgctct tgaatcatct
     9001 ttggctaccc aatgatttat cccgttttct tgcgctaagt attggtcatt ttttttgact
     9061 agaaattgag catctgctag tttttgtttc gtttgccgat ccactttgac aaattgtttg
     9121 cctcctgttt ggatggcaag atgtgtttct actgtttgat tgtcagtcgt caaacgtaca
     9181 tcgttatcaa atgtcgtttt ttcactgttt tctttcaatg tcatttggta acggatcgtt
     9241 aatttttttc cagcaaacga actaagatct tttccagaat ctgataagac aaaaccatgt
     9301 tttatttttt gtgtgtgaaa tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt
     9361 tctggttcta gccataactg aggatcagct gtatctttga tcgtataggt tcctatctcg
     9421 ttgatgttct caggaattgt tacggtgatg agataaggga ctttatcccc atctgcaaaa
     9481 tctgtctgct tattctctac tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg
     9541 ctctttggat acaagtggat ggtcgtcaat ttatgattag atgaatctgt cacaggaagc
     9601 acaacgacaa atggtgctga tttttctttt acttaatctg aagtctttat ttccgtaaat
     9661 cggtaaacag cgtcacgacc ttgctcatct ttgtccgatg cactaaaaga agcaatgccc
     9721 tcttcctcgt ctactgtttt tgtcacattt tctgccagta ttttttcact atcacttttt
     9781 tgtgctagtt ttctttgtgc gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat
     9841 tcatccgaga catcatatat agcgaaagta acgccgttca acccttttac tccttgaaag
     9901 ttatccggga tatttattcc gtcgttttga ctgatacttg gcatcttatc gctttcgaac
     9961 attcgattat gcaaagtaaa tgtaataggt gattctctag ctgacacagt cggagaacct
    10021 ataaaaaaga ccagtaggat aaaaaagaga acagcaaata tatccacacg tacatttttt
    10081 gtttttttca tcgctgttca cctgcttttt tcttacgatg gataaaataa aagaagccag
    10141 aaaaaataaa gatcagtcct aaaaagacaa agggcagtag acctatccca cctgtcgaag
    10201 gaagcaagag agtcggattg atcgtcaact tagcggtatc tgttgcgata atcgtataat
    10261 tattgtttac acttccttcg cctaaactaa tcgttactat ttgtgatcca atatctgtcc
    10321 ggctgttaac tgttagggta taatccaatg attcgcctaa gacaacaccg gtaacgtgga
    10381 gatttccaac cgaaatcgag tgactttggg catcataatt aaaggcagcg tcatcgacag
    10441 taatcactgc gttagcttta tagatggtat aagttcctga tgtgttggct atatcagtaa
    10501 atgacaactt gggatttgct gctttgactt tgtctattcc gctattcgtt agtttaattg
    10561 tatagctgcc agcattaacc atattttttg ctgattgtgc atctgctgtg taatccatac
    10621 tgctaagtgc cacttgtact ggagtaagcg aactagtgac accctctgct agcttgacat
    10681 ggattgacgg gacacctgct tttccatcat atgtcttact gccactgagc tctaccgcac
    10741 cgctgatttg cttaatcgta tagacatcag atgtctgcac gatagtaaat aaatagtttg
    10801 ttctggcagc tagtttagct agtccgcttt ttgtcaattg ccaagacgta taggttccaa
    10861 tttcagtagg cgcagttact tcaactccct tttcattcat cgctttgaaa tcagttggat
    10921 tccatgtgat ttgatcaata ctgagatcat tgtttaaaag ggatgttgat tctgttttat
    10981 cattaaaggc atatttaacg atcggtacaa cagaaagacc atcatagtac ttactgccat
    11041 ttgcattgat tgatactgtt gcatttgtct tataatatag gatgacttca gaaatctgtt
    11101 ttccatttgc gtcctttacg attacatttc catttgtatc taaactagct aaaacactca
    11161 ttgctgattg gttctttgtt tctatcgctg aaacagctgt aaaggtatat ccgttgatag
    11221 taggcgtatt agtcgtcagg tcttgtgcag cacttccgta agtaagattc attattttag
    11281 aaggagcaat catctgtcca aaatagtctt gatacttaat agtgattggt gtttgtttgc
    11341 ctgtataggt caccgtatcg acgttacttt ctagggctgt gaaagaaccg tttgtttgtg
    11401 caaaagaata gcccgttact gctgttggag tgataaccac tttgtcaccc acatggattc
    11461 cagataatgt agtcccgtac gttccgattt ttgctgctcc accattgact gtataagaaa
    11521 aagttcctgt ccgattcgca ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg
    11581 aaacatttcc cttaccatca acgatctgcc catttttgat cgagtacccc gtgaatggaa
    11641 tatcatttgc taactgtata ccgattgaaa aagtactcat acctcctccg gcaggaagcc
    11701 ctccccatgt tgagggtgta aaagaataac ttttatccgc attgggtgta actgaatcga
    11761 tcgtatttcc agcggtagcc acccccatat agatactttt tgggaatgtg atagtagtac
    11821 catttactgt ggagccaata aacagattat ggatatctgg acggttatca ataaaagcga
    11881 ttgctttggt tacgccttgt aacgcagttt tatccgcact catcgatccg ttaaccgcaa
    11941 caatgactcc atctttggca tatatcactg tatatccatc tggtatggta tgtccgttga
    12001 aattcgcagt ggccgcaaca gtactaaatg tctggcttct acctgtttct gaataagctt
    12061 tctcccctgc tgcttttgct gctgtgattt ctttttctgt cggatcaatc agttgagtcg
    12121 aggatgtggt tgaggttgtc tcgttgttgc tagactgatt ggtgctttct atggactgta
    12181 tttcagaata gggtggacta tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt
    12241 ctgtataaac gtttacataa atacttgata agctccctaa gaaagtaaaa atcaagatga
    12301 tcgaaatcat aatgggatgt aagtattttc ttttagatac agctttgagt ctagaaacaa
    12361 aatgtttttg acatatacgt tggattttat tttgagtttt cttaatcatt ttcatccctc
    12421 ctctcaataa a
//
LOCUS       DAZUKT010000083        12423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000083 DAZUKT010000000
VERSION     DAZUKT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.085"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..403)
                     /locus_tag="ACG816_002359"
     CDS             complement(<1..403)
                     /locus_tag="ACG816_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4921208.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQK"
     gene            complement(400..1053)
                     /locus_tag="ACG816_002360"
     CDS             complement(400..1053)
                     /locus_tag="ACG816_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4921209.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1341..1861
                     /locus_tag="ACG816_002361"
                     /pseudo
     CDS             1341..1861
                     /locus_tag="ACG816_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1859..2578)
                     /locus_tag="ACG816_002362"
                     /pseudo
     CDS             complement(1859..2578)
                     /locus_tag="ACG816_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2529..2738)
                     /locus_tag="ACG816_002363"
     CDS             complement(2529..2738)
                     /locus_tag="ACG816_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921210.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(2720..2788)
                     /locus_tag="ACG816_002364"
                     /pseudo
     CDS             complement(2720..2788)
                     /locus_tag="ACG816_002364"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3015..4288
                     /locus_tag="ACG816_002365"
                     /pseudo
     CDS             3015..4288
                     /locus_tag="ACG816_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4395..5426)
                     /locus_tag="ACG816_002366"
     CDS             complement(4395..5426)
                     /locus_tag="ACG816_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921211.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5433..6263)
                     /locus_tag="ACG816_002367"
     CDS             complement(5433..6263)
                     /locus_tag="ACG816_002367"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921212.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6260..7075)
                     /locus_tag="ACG816_002368"
     CDS             complement(6260..7075)
                     /locus_tag="ACG816_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921213.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7072..7896)
                     /locus_tag="ACG816_002369"
     CDS             complement(7072..7896)
                     /locus_tag="ACG816_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFS4921214.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7886..8986)
                     /locus_tag="ACG816_002370"
     CDS             complement(7886..8986)
                     /locus_tag="ACG816_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4921215.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9164..9949
                     /locus_tag="ACG816_002371"
     CDS             9164..9949
                     /locus_tag="ACG816_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4921216.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9982..10365
                     /locus_tag="ACG816_002372"
     CDS             9982..10365
                     /locus_tag="ACG816_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4921217.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10441..10572
                     /locus_tag="ACG816_002373"
     CDS             10441..10572
                     /locus_tag="ACG816_002373"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4921218.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10541..10777
                     /locus_tag="ACG816_002374"
     CDS             10541..10777
                     /locus_tag="ACG816_002374"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4921219.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10789..10956
                     /locus_tag="ACG816_002375"
     CDS             10789..10956
                     /locus_tag="ACG816_002375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4921220.1"
                     /translation="MELGGLPELDGRYTVFGQVYQGLEIVAAIAAQPTNSEDVPIEPV
                     FIRQINFEKTS"
     gene            complement(11021..11830)
                     /locus_tag="ACG816_002376"
     CDS             complement(11021..11830)
                     /locus_tag="ACG816_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS4921221.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLFIESKLYLMDK"
BASE COUNT         3730 a         2791 c         2242 g         3660 t
ORIGIN      
        1 ctttttgcat atcagctgta gaaacaaatt gtagatttgt ttttttacca tcattaagct
       61 ttatttctct ttcttcaatt atcaaagaat tactatcact ttctaaatta acatttatat
      121 tatctttgat ttgtatttca ttcttattat ttttttcttt tatgaatact tttatttcac
      181 tactcttgga ataaaactcc aaagtttgtt ctatatattt tatttctttt ccattcatat
      241 tactagaaat ttcatttgcc atattatgaa tcttttcttt tctcgtctcc aaatatgttt
      301 taggaaagat aaaaaaaatt aatgaatgaa ctaatataat tattattcca agaacagaaa
      361 atatttgtat gaacattttt ggaaatatct ttaatttttt cattttctct ccaatttata
      421 tcctacattt cttatagtag ttatacagtc taattgtaat ttttttctaa gttcttttat
      481 atatacatct attactcgat cataaggagt ttcttcgcta tctttccata cataatcaat
      541 aatttgcatt cttgttaaca cttgtccatt attatccaat aaacatttta atacttctag
      601 ttcttttgca tttacatcta tttcttcacc atttatttta gctgtatagc tattaaaatt
      661 aaccgataga tccttatatt caaacttctc taaatgtcca taattcttct taattaaaga
      721 atctattcta gcttttaaga ccggcaatga aaatggcttt tctacatatc cgtctactaa
      781 attagtaaat gcatcaattt tatattcttc atcactaaat gcagtcaaga ttagaattgg
      841 taaattgctt ttctttctaa tttctttcaa cacttctaaa ccatttataa aaggtatctg
      901 aatgtctaag ataaccaaat ttatatcgct attaaatttt gacaaggctt ctcttccatc
      961 tttagcttga ataacagtat atccaaattc tgaaagatat tcacttattc cctctcttat
     1021 catattatca tcttcaacag ttaatatttt catatatacc tccacttaag cctatacact
     1081 agatattata cagcatatta tggctctata atatctagtg ttggtgacgc cttctgttgc
     1141 caatactttt tctttttttt aataaatgca aacagacacc ttgaaatcct ttagaaaaaa
     1201 ggcttctata atattttgtg ttgttggaaa atcgaaggcg attttctact aacacttttt
     1261 cttcgtttca actcaaaaag agactcactt ttggtaaaat ttagtcgacc aaaacatcac
     1321 cctaccaaag gagtcgctct atggataatc atactagaaa attactcgga ttaacagata
     1381 aacatttatt ttttgatgaa gaatggctga ttgaaaaaga atataaaggc gttcaagcgc
     1441 agtttattaa agggagattg gatgactcac cttcccattg tgaacactgt ggatcgattc
     1501 gtattatccg caacggttcc tatacgacac gtactcagat gttgaaagtc aaagaaaagt
     1561 tgactatttt agaattaaaa cgtacccgtt ttctatgcta tgattgtggg cagacctttt
     1621 ctgcgaaaac ggacttagtt gatgaacatc atcaactaac aaaggaactg aaacaggcga
     1681 ttctcataga actgtacgaa aatcaatcac gtaatctgaa aatcagaaga agtaccgtcg
     1741 gctcaagcga tattggaaac tcttactcaa agattccacg acattggagc cgctcaaacg
     1801 gcattatcat cgactgttta aacgaccaat tagtcaaaca gaaatcgtcg atgaaccgtt
     1861 gtgcacaatg gtgtcctgaa cgtacagcga caccttccat atccagagca gtcgccacat
     1921 cacggggatg gatgccatcc aaattaaaag caaccacgcc agtatgatgt tcaggatctt
     1981 gcggtccgta aatggtgata ccgccgatcg cttgtaattt aggtaaaacg taggcaacca
     2041 gttcttgctc ataacgatga atattctcca taccaatctc attcaagtaa tcgattgctc
     2101 ctgcaatgtt cggagtacca gcttcaaatt tccatggcag ctctttccat gaactatcgt
     2161 aaagattgac aaaatctatc atttcaccgc cgaattcgac tggctccatt tgttctaaca
     2221 attgacgttt gccatataat acaccgattc ctgtcggccc gcacatttta tgcccgctaa
     2281 aggcgaaaaa atctgcatcc aacgcttgta catcaactgt catatgcggt gctgcttggg
     2341 caccgactgg actatcctca actacaacag cctgttcaac cctcagccgt ttcaagcaca
     2401 acaaataacc ttctggatct ggtttgccga cctgaagatc atcgatcgtc acaaaggaat
     2461 caattttttc cttaatcatc ggatgtgctt caaaaaaata gtctagctcc cgcagttcag
     2521 aattgagact actccgattt tccagtgctg ctgatccgct gctcaaaatg ttcagcatcc
     2581 actggtctcc caaccgcttg acttatccct tcataaaagg acagttctgc tgccgtccca
     2641 acaccttgtt gatagtctgc aaaaggaatt ttatagccga actgctccag taaataggct
     2701 tcagtaaata gtgatgaata ctctcgtaaa acatccattc actgtcaatc atcgtcccat
     2761 caaaatcaaa aataagtgct ttggtcatct tctttccccc ttacccaaaa caagcaaaag
     2821 ctgtgtcctg ctctctctac agtatcagca taaaaactgc cgtctgaaaa gtcaaaggtg
     2881 aaagaaatgt ggcgctataa tttctaggac tgatagtaaa tcattctatc agtccttttg
     2941 agcataaaaa aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca
     3001 aaggagcgtt ctgtatgcct gatcttcatc ttatcaaact attactagga atcaaagatg
     3061 gaaacattca aatcactcat gtcgagaaga aaaaaataca ttcaatcaac agtatcgttg
     3121 tttctgccac gctcattaaa acgattcagc cctgtcctca gtgtaaacag gtgaatcaac
     3181 atgggctgat cgttaagaac ggaaagaaaa aatcgctggt ccttctcaat aagtgtgcga
     3241 atcaacggac ttatcttgct ttggcaaagc aacggtacct ctgtcgtggc tgtcacacct
     3301 attttacggc gaatacctac attgttgaaa aaaactgttt tatcgccaaa caagtacatt
     3361 ataaaatatt ggaggaattg actgaaaaac aagcaatgac gacgatttct aaacattgcg
     3421 gcgtttcttg gtcaaccgtt tcgcgaacac tcgcttctct gctccccatg acaaacgtga
     3481 aaataaattg gttgcctcgc tgttcattgg tagatgaatt tcgttcattg aaaaatcaag
     3541 tcggtcccta ttctttttct tgtatggatg gcgatactgg gaaattactg gatattcttc
     3601 cttcacgtaa gaagaaagat ctcatttctt attttatgca atttgaacga cgtgcacgtt
     3661 taaacgtaaa aatactcgta acagatatga acgcctctta tgctttgctg ataaaagagt
     3721 gctttcctca tgccaaactc gttgtggatc gctttcacat cgtcaagcac ctcattcgca
     3781 gttttaagga tatttgacta tgagtcatga aaagcttcga ccgaaacgat ccaatacaag
     3841 ccaaacacta tcgacaagtg aaggctttat ctcgacttct gatcaaacga caggacacgc
     3901 ttgtttatga taaatggaca aaatggcgta attttggttg ggcctattta accgaaagtg
     3961 aagtcgtaga acgtctactt tctacttctg atgaactacg aatagcttat gcttactatc
     4021 aagaaattct tcaagctttt tatgacaaag aagcggacat cttctttcag cttgtaaaaa
     4081 caatgcccaa gtcggttcca agagaactgc atcatataaa aaaagcgttt ataaactatg
     4141 aatccggcat tcgtttagcc ttagagttgc cttactcaaa tggcaaaata gaaaatctgc
     4201 atacccacat caaagcattg aaacgaatag cttacgggtt aggatgagaa ccagaatttt
     4261 cttaatcaat catctgatca cttattaaac ataaaaaaac atctaaagca gaaaatcaat
     4321 tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag ccagaaatgt
     4381 ttgccgacta tacactagct ttgttgtgca aaatgctgag tatcaatttc cacagggatt
     4441 gctagaatat ctttcagctg aatgtataag gcatcgctaa ttttctcttc ccgatcgttc
     4501 aaccgatcca ctaaaatttc tttgccctcc agcgtttgaa aaacataccg ttgaatgttg
     4561 cctaaaataa atgattcctt ccatagaacc ggaatcttaa ccccctgagg aaccggttca
     4621 aattgaatca gctccggtct taaataataa atgcagcgat tgcccttagc ggaaaactcg
     4681 tgggtatact gattcagctc ataccattcg aaacgattat agttgccgat aaattccgca
     4741 acaaatcggc tctgaggatt ctgatacaat tccttcgagg tggaaacctg agccacccgc
     4801 ccagcttcca tcacaaaaat ccgatcactg attcgcatcg cctcttcttg gtcatgagtc
     4861 acaaaaatca tcgtcatccc tagcgattgc tggatagcgc gcaaatcccg ctgcagctgt
     4921 tttctaattc gggcatccaa agcacttaat ggctcatcca gcagcaggac tttcggttca
     4981 gtgaccaatg agcgagccag tgccacccgt tgtcgttgac cgccagaaag aaactggaca
     5041 tttttatctg cctgctccgt catatccacc aatgatagca tctcaaaaac cttttcctga
     5101 atcacctcag gactcagctt ctttaccttc aaaccaaagg ctacattttc aaaaacagtc
     5161 atagttggaa acaatgcata ctgttgaaaa acaaagccga tattgcgatc cttcgtaggc
     5221 ttatcggcaa tattctcatt gtccagcaca atctcaccgc tctttggttg ttccaatccc
     5281 gtgatacagc gcagcaacgt agactttccg cacccgctgg gacctagtaa agtaaccagc
     5341 tcaccctttt caatctgaat ttcatcaata gctaaaatct gtttttcatc aaaaaacaga
     5401 ttgagatcat gtatttctaa aaacattttc cgtcacaact cctttaaggg cttctttttc
     5461 gactgcttct cttccctcgt ctgcttaagg gacagctgtt gcttttgtcg ataagtcagc
     5521 ataaaggtag ttaaagcaat tcccaccata aacagcaaat acaaaaccat cacagcgcta
     5581 gccaaatggc cgttttcgtt catccgccgc agcatataga tgcgtaccgt ctgaaatcga
     5641 ccgccaatca acaggttcgt cagcatgtac tccccaaaaa ccgaagaaaa aatcatcaga
     5701 gaaactaggg tcacaccgat acggatgttt ggaaacagca cctggataat aatcgtagac
     5761 atcggggcgc ccaaggtttc tgctgcatcc actaattcct tcagattaat taagcgcatc
     5821 tggttgttaa gactaagata gacaatcggc aacgccgtaa taaacaatgc acctaccaac
     5881 actacaaaca ttgggatacc agtttcagca tacgttcgaa gcaaagccgt taccaaaatc
     5941 actcctggta aagcatacgg cagcaggagc agcttttcca gccaacgatt caaacgaggg
     6001 aaatacaaat gaatccaaat aatggtggga atcatcaaca acagaatcgt caccaagacc
     6061 acacctgcta ataaaagtga gcgacccacc gcagcaataa attccctgtc cgtcagcagc
     6121 tgttggaacc attgcaaggt aaaatcacta ggtaaaagcg attttgacca tttagtggag
     6181 ctggcataca aaatcgttcc aataatcggc agcaagtaat acaggacaaa gcagaacaaa
     6241 atcggtttta ttaacttttt cacgtctgca ccaccttgcc tttcttttga ataatcaaca
     6301 ttgccgttac cagcaggaca cctaaagcga ctgccaacgc actgcctaaa ttaggcttgg
     6361 cgaacacatc tcccgacact aaagccgcaa ttcgaatcgt taaaagattt acattactgc
     6421 tggtcagggc atacgctgtt tcataggtcc ccatgccatt agcaaataag atgacaaagg
     6481 tgctggcaat cgatggtcgc aaaaaaggca aagccacctt gaaccaagca tacgctgatg
     6541 aagcgcccag caattgtgcg gactccagcc attccttttt cacttccttc atgctgggat
     6601 acaaaaacaa gacccctaac ggaatttgga aataggtata cgtgatggtc aatccccacc
     6661 aagaatacaa attgaagtcc gctagaaatg gcaaaatcat tttcagcaca cctacattcc
     6721 ccaacaaaat aataaaagag aatgccagcg gaatcccagc aaaattagct gccaaattgg
     6781 aaacaaaggt gattttctcc tgcaatttag tcgatagatt cattaagcat aaacataaaa
     6841 taaacgtccc gattaaccca atcacactgg agctgacagc aatcagtacg ctgttaacaa
     6901 aggcctgtgt gtaatatttg ttgctgaaaa tatcactgta attggccaat gtccactggt
     6961 tagttaaatc actgtgcaag ctgcacagaa ccataaaaag cagcggcagc agtaaaaatg
     7021 cacagaagat aaaggaggca ggaaccagaa ccaagcgttt tttcatctct ttcatcccct
     7081 catcaattct tgaatgtcct gcattttctc tgacggtgca attcctaata accagcaaaa
     7141 caatggagcc gccagcagct gtggtacagt ttgttccttc agcttttggc gtttatttga
     7201 aatcaaataa aaaggaactt cacgatgtgc agccaagctg ccgccatgca gtccgtgcgc
     7261 atccatcccg tgatccgcag tcaccacgac ttgataacct ttttgcagcc attcaggaag
     7321 gtaaatagcc aataatctgt ccgcctgatt aaccgctgct gcatattcat gagaatcggc
     7381 ggtaaatttg tgaccgacat catcaatatt catcgaatgt acgaccaaaa agtctggctg
     7441 atactgattc actaaataat gggcatccgc aaataaatgc gaatcagggt aagtgtcctc
     7501 actataaaag atgccatgct gaatcagtcg ctcctcctga tgctggatgc gatcaaccaa
     7561 acgattatag ggggcttgat tgtacaactc gctgtaccag tggtaagcgg cggctcctgt
     7621 ttttccgcca gcttccttaa ctaattcaaa cagcgaccac tctttggatc gctgagcatg
     7681 gtaattacta tagatgccat tttcataggc tggaacgccg gtcatcagta cttcgtataa
     7741 cggacgtgaa ttgctgggca gctcactttt taccttcaat aagcttgctt gctggtgctc
     7801 aactaaatga ttcatgaacc caagctgggc gacggctgtg tcgtagcgac agccgtctag
     7861 tataactaca atcagtttgt caagtctact ttgcataggt cagtacctcc gcctgccaaa
     7921 gctctggaag ctctacagag gtcttgttcc aagcttcatt gtcttcaact ggcaccgccg
     7981 cttcataagc atcctccggc agtaattttt cagctacttc tttaggcagc tctaccttgc
     8041 gaataggtcg cgcatagccc tttgccaaat tgatttgccc ttcatcagat aaaatatatt
     8101 cccgtgctag ctttgccgca ttgggatgag gggcattttt attgattaag gttgtatacc
     8161 cgctaatgat ggagccgtct gttggcacag ttacctcaaa acgctcggag tcaatttggt
     8221 cacgataatt tagtccattg aagtcccaaa caactgccac atcgatttca cctttttcaa
     8281 gatttgctac gctggcatca acggtggaca aacggccttt ttctgccatt tttttgtagt
     8341 aattcagacc tggctgaata tccttttcgt cacctccgtt ggcaatcgcc gcagccaata
     8401 cagcaaattg tgcttggttg gcaatcgtca catcccccac gctaacttga tagtcaccct
     8461 cttgcagctc cttccagcta gtcggtgctt gcttcacatt ctctttatcg gtgataaagg
     8521 aaatggttcc cgtatagctt aacaaccaat ccccatcatc atcctttgcc caatccggaa
     8581 tttcctccca atagttggtc ttataaggta aggttaactc tttgctttca gccaacggac
     8641 cgaagctgat accgacatca ccaatatcgg cggttccatt ttttccttct gattcaaact
     8701 tagccaattc ttctgcactg gacatatccg tatccacgtg acttaagccg tattcttttt
     8761 caaggtcgtt ccaagtgcct acccaatttg cccaagtatc cggcattccc aagctggcaa
     8821 tctctccttc ttccttggct ttttctataa tcgtatctaa agagtcgtct gccgcagccg
     8881 attgcttcgt tgcttcacct gagccgcagc ctgtcagcag gatacttact gttgctgcac
     8941 aaaataaacc gatagtcatg ttacgaattt tttcattcat tttcattcat tcttctcccc
     9001 tttcgataat tccattctaa caaagctttg taatgagaga atcagtttct tgaaaaggtt
     9061 tcatgaacaa tgtaaaggtt gtatatggtt tgtaaatttc gacgaattcc cgttaacctt
     9121 ctaaagcttt tgcggtatga tagtagaaaa aaggaggctc cctatgcagt attatgatca
     9181 gcatctccac acgtattttt cctttgattc agaggaaaaa tttgaaaact atttagccta
     9241 tcagccggag tttttcgttt ctacggatca ctttgattta aacaatccat ctaccggaaa
     9301 agatgacatt ccggattatc cagcctatat aaacaaactg gactctttaa aagaaaccta
     9361 ttccaccaaa tttctgcgag gtatcgagat tggcgttgtt ccaggacaag aagagcggat
     9421 cgtggattac ctcgctcagc atccttatga tttaaagctg gtaagtattc atcaaaatgg
     9481 ccgctttgat tatatggacg atgtggtttt aaccaaagat cccattcaaa ctaccaagga
     9541 atattttgat cagatgttgc aggtcttgac ggattttcca gaaggagata ttttgactca
     9601 ctttgattat ggattacgcc gcttcacctt cacagcggaa gaattcgcgg agcattttga
     9661 ggctcagctg ctaacgattt tcaaaaaggt tgttgaacga gaattggcga tggagttaaa
     9721 tgccaagagc ttcttgaaat acggcaacga agacctttac cgttatgccg ttcctttgta
     9781 tcaaagtgtc ggcggaaagc tctttacctt aggctcggat gctcatgttg ccgaagatta
     9841 tcagctgggc tttaaaaaaa tgagtcagct gttggcagac tgtcaggtag aaaagttgct
     9901 agtcattcaa ggaaaagaac gccgcctgac gccgctgccg attttgtgat tcctatataa
     9961 tagaagaaag tggtgaaaaa aatgagcaac ctgtgggact acaatcaaga ggcacccatc
    10021 cattatttaa ttgcgcgaca ttgggatgcg ttgaaaattg aggcggtttg ccgatccttg
    10081 ttagcagccg ttccaaaaca acaactggaa aattttttgg tggctgacag cttgcagcgg
    10141 gaaaaagttc aggcttattt tgcagccttt aaagatcagc ctttggaata tttgcatgcg
    10201 cagtttcatc ttttttatca agtagccgcc ccagatgact acaacgattt acgaggacag
    10261 ctgcaattga cctttcaagc ggatgaaacc gcctataccg ttttattggg aatggctcgc
    10321 cttggtgatc aggcgaaggt cgagtggcgg attttcgata tttgatggca ggatcaaggc
    10381 gtttcagttg caaaccttcc tctctttcaa ttagaaaaac cgcagcagga agaagtttgt
    10441 gtgctggtca ccgatgctgg tgaaattcgt ctccgcttat ttcctgacca agctcccctg
    10501 gctgtagcca actggcgctc cctagctgag caaggcgttt atgacaacac acctttcggc
    10561 tcgtgtcatt aaggaatttg ttattcaagg tggcgctttg gatggttctg gtgatgaagc
    10621 tgtttccagc tatgagggct ttttcgctga tgaagtgcat cgcggacttt atcattttaa
    10681 tggcgccttg gccttagggg accacggccc ccacaccaac ggcaaccagt tttttattgt
    10741 gcaaaacaca aaggcgcagg ctgacctgct aatgtaaagc aagcatactt ggagcttgga
    10801 ggactaccgg agctggatgg tcgttatact gtttttggac aggtatacca agggttagaa
    10861 atcgtagcag cgatcgcagc tcagccgaca aacagtgaag atgtaccgat tgagccagtg
    10921 ttcattcgac aaattaactt cgaaaaaaca tcgtaaaaag acactcactg acttcagcaa
    10981 aagctctatt cttgagctgg ctgcagctag tgagtgtctt tcatttatcc ataagataaa
    11041 gcttggattc aataaataaa taaataaatg ttttggttaa agctgtgacc agcaaatgag
    11101 gaaccgtgcg agccagttga tctgccaatg caaacatttc tttaggtgaa ggcgctggcg
    11161 tgtctgccaa cggatactcc agtcccaatg cttggtaacc gtgcatacca tagcagtggt
    11221 acggtagtaa ttctaacggt acgaaccgat caagatctgc cacaaaacaa gcaatcgtca
    11281 gcagctcggt caggttcata ttgatacctg gaaccacagg aactcgaatt cgcacttcgc
    11341 agttaggata ctcaattaaa aagcgacgaa tattcttcaa aatttgttgg ttatctaagc
    11401 cggtccattg tttgtggagc ttgctggtaa acatttttac atccacaaac aatgtatcca
    11461 catagggagc taccttttgg atttcattat aagcaccaca aaagctggtt tccagtacgg
    11521 tattgattcc aatgtatttc gatttttgca aaatttcttt agcaaaatcc gcctgaacca
    11581 gtgcctcgcc tccgctaatg gtcactccgc catccgagtg aaagtaaaaa acagcatcct
    11641 tctcaatctc atccattacc tcttcagcgg tcatggtctg gccaaatcct ttttcaatct
    11701 gcttgctttg tgattcggga atcgaacacc aggcacagcg taatgggcag ccctttaaat
    11761 aaacaacggt ccgtaaacca gcaccatcat gtagagaacc ctgttctatt cttaatactg
    11821 ttccttccat atagattttt gaattacaca ctagtttaaa ctaaaacaca caaggaaaaa
    11881 cttaccattt aatcataaaa ttagctcata taaatatact aggatgtaaa atagactggt
    11941 caaaatctgg tcaaaaaaga ggactaatac aggtagtcct cttttttgtt attttaatat
    12001 tctaaatcat tttttattgg tttattccct tattcgatgt agactcaaat cactatatca
    12061 ctagatatca aacggcgtca tacggaatca aaaacatcaa atttgacctg ttaaatggtc
    12121 attgaataac attcaaaatc aataaaacgg agtcagacac ggagtcaaaa acgctgagag
    12181 taaaaaatat atacagcctt gtaggtaaag taccacaggg ctgtatattt tttatttcag
    12241 attaccttaa atgattccat ttacttttta tctcattttt tcctactagt tagtatcaaa
    12301 tactctccta ttttctcaat aaactatttt tggatacatg gtatctgaaa cagctaaaac
    12361 cttgtcatta cagcaaaaaa tcacgttatc cagactgtag acaaagtcca ttttatggat
    12421 gat
//
LOCUS       DAZUKT010000084        12290 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000084 DAZUKT010000000
VERSION     DAZUKT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.086"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACG816_002377"
     CDS             complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACG816_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4921222.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACG816_002378"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACG816_002378"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4921223.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACG816_002379"
     CDS             complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACG816_002379"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4921224.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="ACG816_002380"
     CDS             5737..7038
                     /locus_tag="ACG816_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4921225.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="ACG816_002381"
     CDS             complement(7107..7706)
                     /locus_tag="ACG816_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4921226.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7961..8842
                     /locus_tag="ACG816_002382"
     CDS             7961..8842
                     /locus_tag="ACG816_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4921227.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8855..10465
                     /locus_tag="ACG816_002383"
     CDS             8855..10465
                     /locus_tag="ACG816_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921228.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10542..11201)
                     /locus_tag="ACG816_002384"
     CDS             complement(10542..11201)
                     /locus_tag="ACG816_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4921229.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11484..12149)
                     /locus_tag="ACG816_002385"
     CDS             complement(11484..12149)
                     /locus_tag="ACG816_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4921230.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         3538 a         2458 c         2245 g         4049 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     7861 aaaataataa aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
     7921 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
     7981 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
     8041 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
     8101 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
     8161 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
     8221 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
     8281 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
     8341 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
     8401 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
     8461 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
     8521 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
     8581 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
     8641 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
     8701 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
     8761 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     8821 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     8881 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     8941 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     9001 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     9061 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     9121 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     9181 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     9241 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     9301 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     9361 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     9421 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     9481 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     9541 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     9601 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     9661 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     9721 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     9781 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     9841 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     9901 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     9961 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
    10021 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
    10081 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
    10141 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
    10201 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
    10261 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
    10321 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
    10381 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
    10441 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
    10501 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
    10561 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
    10621 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
    10681 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
    10741 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
    10801 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
    10861 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
    10921 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
    10981 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
    11041 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
    11101 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
    11161 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
    11221 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
    11281 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
    11341 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
    11401 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
    11461 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
    11521 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
    11581 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
    11641 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
    11701 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
    11761 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
    11821 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
    11881 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
    11941 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
    12001 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
    12061 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
    12121 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
    12181 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
    12241 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat
//
LOCUS       DAZUKT010000085        12143 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000085 DAZUKT010000000
VERSION     DAZUKT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.087"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..565
                     /locus_tag="ACG816_002386"
     CDS             <1..565
                     /locus_tag="ACG816_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4921231.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            966..1571
                     /gene="fic"
                     /locus_tag="ACG816_002387"
     CDS             966..1571
                     /gene="fic"
                     /locus_tag="ACG816_002387"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4921232.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1587..2159
                     /locus_tag="ACG816_002388"
     CDS             1587..2159
                     /locus_tag="ACG816_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4921233.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2411..2668)
                     /locus_tag="ACG816_002389"
     CDS             complement(2411..2668)
                     /locus_tag="ACG816_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921234.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2794..3609)
                     /locus_tag="ACG816_002390"
     CDS             complement(2794..3609)
                     /locus_tag="ACG816_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921235.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4126..5451
                     /locus_tag="ACG816_002391"
     CDS             4126..5451
                     /locus_tag="ACG816_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4921236.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5444..5794
                     /locus_tag="ACG816_002392"
     CDS             5444..5794
                     /locus_tag="ACG816_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921237.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5791..5961
                     /locus_tag="ACG816_002393"
     CDS             5791..5961
                     /locus_tag="ACG816_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921238.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6865..7140)
                     /gene="prgO"
                     /locus_tag="ACG816_002394"
     CDS             complement(6865..7140)
                     /gene="prgO"
                     /locus_tag="ACG816_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS4921239.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7112..8065)
                     /locus_tag="ACG816_002395"
     CDS             complement(7112..8065)
                     /locus_tag="ACG816_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4921240.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8677..10170
                     /locus_tag="ACG816_002396"
     CDS             8677..10170
                     /locus_tag="ACG816_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS4921241.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10305..10601
                     /locus_tag="ACG816_002397"
     CDS             10305..10601
                     /locus_tag="ACG816_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4921242.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10702..11553
                     /locus_tag="ACG816_002398"
     CDS             10702..11553
                     /locus_tag="ACG816_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921243.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11574..11786
                     /locus_tag="ACG816_002399"
     CDS             11574..11786
                     /locus_tag="ACG816_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921244.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4170 a         1864 c         2268 g         3841 t
ORIGIN      
        1 ttattttaag tctgaaaagg atacatcgtc tctaacgtta actttagagg aaggagttga
       61 acctgacaag gtgacctcta aagtgataaa ccaattgaaa gaaaaaggta gtttgaaaca
      121 tttgggggaa tatgaagtgt tagatacggc tatgttaacg aaaggaattg gtcaaatcct
      181 cagtacgatc acttatttta taacagctgt agcaggtata tctttattta tcgcaggggt
      241 aggtgtaatg aacatgatgt atatatctgt atctgaacga acaaaagaga tcggtataag
      301 aagagcccta ggagctactc gaaaatcgat tatgctgcag ttccttttag aaggtttgat
      361 tttaactctt tcaggagggt taattggcta tttattagga atggctcttg cttatagtgt
      421 tggttctttg ataaaggtaa acgtgtcact tgatctattt actattttac tagcggttgg
      481 ggtatcttcg gttattggac tggtcttttc tgtaatgccc gcttcagagg ctgcaaaaaa
      541 agatttaatt gatattctta gataaaatca gaatgacaag aatatttacg ttctagattc
      601 taaaataaaa cacaaagaga ccaaccaggc accctattgt ttctttgtag tatagagata
      661 ttctttggct tgtaagagat caatagagtt aggtgaccgg agttcgaaat aaaggaaatt
      721 tttgattgta taggagaatt ctttatccct aatatttaga attttgagta tgccatgttg
      781 atgttttaga ttctctataa atatagaggt gtatcaaagg tcaactagac tgtgcaaaat
      841 agagacactt gaaaaatcta tcgggggtca gaaacttaat agtttttgac acataaacaa
      901 aatcgcgata taatggggtt gagagtgttt cataaactta tgtcataaag ttaaggagat
      961 ttcgcatgat tttagagaat aaattgggat taaccaatca agttgagttg gctaaggttg
     1021 aagaaaaact cagtaaacaa aaagcgaaag aattgtacga ttgtggaaaa ataaatgaaa
     1081 tcgaagttgg tacatttaaa ggtttatctg aaattcatga atttctattt tcggatattt
     1141 acgattttgc cggaaaaatc cgatcggtca atattgcaaa aggaaatttt cgatttgcac
     1201 cagttatgta tttagaacat tcgttacagc atattgatca gatgccacaa acttcttttg
     1261 aagagattat caagaaatat gtggagatga atatcgctca cccatttcga gaaggaaatg
     1321 gccgaagtac tagaatttgg ctggatctca ttttaaagga agaattgcaa aaagttgtag
     1381 attggaactt aatcgataaa gcagactatc tttctgcaat ggagcgaagt cccataaacg
     1441 atttggaaat acgctatcta atttccaatg ctctgactga taaaattgac gatcgcgagt
     1501 tatatatgaa aggaatcgat gtcagctatt tctatgaagg gtattctgag tatacgatag
     1561 atgatttgta aaagtaggtg gaccaaatga cgaaatatgg atatgcacgt gtctcaaccg
     1621 cttctcaaag tacaaaagaa caaattcaac agttgcttga gaacggcgta gagaagaaga
     1681 atattttttc agaaaaattt acgggtacaa aacgggatcg gccaaaattc aatcagctgt
     1741 tggctggtct gaagcctgga gatgaaatag ttgttacgaa gcttgatcga tttgctcgta
     1801 acacgagaga agcattagag atcattgaac ctttactcga tgataacgta acgatcaagg
     1861 ttttgaatct aggaacgatt gaaaatacgt ccatggggcg aatggtgaca cggacgttgt
     1921 tgagtgtagc agaaatggaa cgtgatatga ttgtggaacg tacccaagaa ggaaaaatgt
     1981 ttgcaagaaa gaataatcct aatttcagag aaggacgtcc gaaagcaaca atcacaccga
     2041 agaagaggca cgcatatgaa ctgcttactt caggtaaatc gtataaggaa gttgaagcta
     2101 taaccggttt cagtcgcagt acgcttttta gaataaagaa gaaaattgaa gaaagttaaa
     2161 aaagaagtct cagcgaacca gacaggtaaa tacttaatgc gttctagata tgagtctatt
     2221 agcatgttgt gaaatataat atttttttat ttacttgatt tctgctcaaa aaatgtgtat
     2281 ggtaactgta aaaggtaaag taattaaagg tatgctagcc acgcttacta tattaggata
     2341 ccatcatata tttataatga acgatttgtt cgtctaccaa acaacacata tcgttcagcg
     2401 taagtcacgt ttaggaacgt ggctttttta gtgttattat cgataaataa tctattgact
     2461 ctattgattt tatctccata tccctacggt gttgattgtc tttagatctt tcatttaaga
     2521 accaaataac tatcataaaa atcagcactg ctgctaatat aataaacagc attcctattg
     2581 tgatattacc tattgatttt accataacta cgattaaagt aataatggca ttcaagtctc
     2641 ctttgttctc tatatataaa ttagccataa atcatcttcc tttcaaatta agatacttgt
     2701 ttgataccaa taaatagtaa gcgtggctag catacctttt tctgtaatag caagtgttct
     2761 aatagtccaa acttaatgat ggaccgatgc tagttatagt acagtaatgt tttctttctt
     2821 atctagattt ttagtaagct ctatcagcaa atctaacaaa tctgagtcct tttcagaaac
     2881 taaattaaat ctagtgttta caacaaagtt ttcatgttct atattgttta gattatttgc
     2941 ggaaaggagg gcttctagat cttgacattc aaatctatat gcatcgtcat aaatcttact
     3001 agaaggagtg cttaattcga aattacttat tttctcatct tggaccaaga taaatttata
     3061 gatagtatct ttttgtgttt ttcttttaaa aacacaacag agtgtgttat aaggaacgtt
     3121 ataattttca tcagcctcat tttttctcca ttcaaacaaa tgataaactg caaccaagtt
     3181 gccatcaatt tcatggacag tgctctcggt aacatccttt aaagaactat caagggctaa
     3241 tttatagtaa ctagagagag gaagtaaaat tgtcgattta ctgagatggt tatcgtctat
     3301 acgtgcaatc atacctaaat caactaaggt tttattccag gtgtttatag ttttagttgt
     3361 cactcctaga gactctgcta tggtattgac cgaatgccag gaatccccgc cttcattctt
     3421 tgccttaaaa cagtaataca gatataaatt aatagctttg ttattcggta aatatttttc
     3481 aagttctgtt ggtagaataa agtaagaatc cttaatgttt tttctgaact gtctccagtc
     3541 tctgtagttt tgtttataaa gttgtgaaac ggtttttcca cctttagaaa gtcctgctat
     3601 cttaaccaat cttattgctc ctttcttgaa tctgtcatga tcatacagta ttttttactt
     3661 gtagtccagc accttaatat attatcctat gtaattaatt tcgtactaga tagtaattat
     3721 tttttattta attctctagt cacttacaaa taagttatat aaactacgat taataaatta
     3781 ttacaaaaac tattaagaaa acttctacat acaactatta caaaactatt aagaaactga
     3841 atactcctat tttttgtttt taaggaaagt tttttctttt ttgaatttct tatttttttc
     3901 taatataact aagcaaaact ggataatacg acacttattt tcaaattttc acaagactaa
     3961 tagtaaatat ccatttaata aagtgataca atgtgttgat tttacaaatc aacagaagaa
     4021 gtagggaaat aacttaccta tgcttctttt tttgtacatt taatcgattc ttctttacaa
     4081 aaacgaacgt atgtttgtat aatgtggaag aatggagtgt tatttatgat ggattattca
     4141 aaagagccag taaatgatta ctttttgatc gatatgaaaa gcttttacgc aagtgtcgaa
     4201 tgtatagaaa ggaatttaga tccattaacg actgaattag tcgtcatgag tcgagcggat
     4261 aatacaggat cagggttgat tttagcttct tcaccagctg caaagaagaa gtacggtatt
     4321 tctaatgtca gtcgaccacg agatctaccg caaccttttc ctaaaacgtt acatgtcgtt
     4381 ccaccacgca tgaatctcta tattaagcga aatatgcagg taaacaatat tttcagaagg
     4441 tatgtagcag acgaagattt attgatttac tcgatcgatg aatcgatctt aaaagtgacc
     4501 agatcaatga atctttttac gactgaagga acaagaagtc aacggagaaa gaagctggct
     4561 caaatgatcc aggagaatat taaagaagag ctaggattgc ttgctacagt aggtgtcgga
     4621 gataatccct tattagctaa acttgcttta gacaatgaag caaaacataa tgatggattc
     4681 cttgctgaat ggacgtatca aaatgttcct gataaagtat ggaatattcc tgaaatgaca
     4741 gacctttggg gtattggatc tcgaatgaaa aaaagactca accaaattgg tatcatgagt
     4801 attaaagact tagctaattg gaatccatac actattaaga gccatttagg tgtgattggc
     4861 ttacagctgt attttcatgc gaacgggatc gatcgaacag atatagcgat cccaccagaa
     4921 ccgacaaagg aaaaatcata tggtaattca caagtgttac cacgagatta taccaggaga
     4981 aatgaaatcg aactggtggt aaaagaaatg gcggaacaag tagctattcg tatcaggcaa
     5041 cataactgta aaacaggttg tgtccattta tacattggca cgtcgatctt agaaacgaga
     5101 ccaggttttt cacaccagat gaagatccct ataacagata atacaaagga attgcagaat
     5161 tactgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca cgttggaata
     5221 acctatagca agcttgtgta cactgattcg cttcagctgg atttatttag tgatccacaa
     5281 aagcaaattg acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
     5341 tttacgtcga ttgtacatgc ttcttctatg ttagaaggcg ctcgtagtat tactagaagt
     5401 acattggttg gtggccatgc aggcggcgca ggagggctta aaaatgattg attataatga
     5461 tcgtggacgt ttaaagtggg caggttttta tttatctgat catactgaaa aaattgatgc
     5521 cgacaatgaa caacgagcca atcaaaattt ggcaaaagaa caaatgacga ctgaagaggt
     5581 tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatgtattc aaaaagaaga
     5641 acgaaacgct gaaggatact atccagaaga tattgttagc aagatcttag gtagtgatga
     5701 gctggggatc tacgttggaa aaaacaaagt tcactacgac gaaatacgtc atatcgaatt
     5761 ttaccaggat aataaatggg gtcgattaga atgaacatta tccagcaata tgagctaaag
     5821 tatataacct ttgatcagct atcagaagag atttggggtt atggccagcg tttgataaat
     5881 gaggttggtg ttgaacgctt ctctttctat gtagaagctg cagcaggata ccataaatac
     5941 gaatactata ttggctatta aagtgcaata aaattatatt tcttttatgt tttacatttt
     6001 gtatcaattc tgataaaatt acctataggt aattaaaaaa tataaaaaaa ctagttacaa
     6061 ccttaggacg ttgtaactag cttgaagcat actagagcta actaaaatag cactagaaag
     6121 caatgttaaa cccgtccaac aaacttgatt taacattgag accagcttat tcagctaaat
     6181 atagttgaat aagcttcttt tgttttaaaa aaacaaaagg tgtactacga ataacgcaag
     6241 taaaagaaat actattacaa ccagtaggct acaagtaagt attactaaac attgcgtagt
     6301 tagaaccttt gctctattgt ctaaagccac aaaattagag atcagcttta tgatgctaag
     6361 agcgagctgc atccatgcgg acataggttc ttttgacact attaattctc ctttttagga
     6421 taagggaaat tgctagtagt tttttcatta gcaccatccc ccttcatttg attgagtcta
     6481 gtgctgctgt gatagtttgc tgtttaatat gcttacattc ttattataac ataaatgaaa
     6541 caaacacatg gttagaggtc gtatcaatca aaatagtgtc tcacaaacga tttattactt
     6601 actcctgctg actaacggac tgttcttcag taactgtaac tatatgtttg ttaaaaccac
     6661 cattacctgt agataaaaac caatatattt actatactca ctatattaaa tatatttagt
     6721 atagtaaaaa ctagaaacaa taaaatttgt gaatgttgta tacccccaaa caatacacat
     6781 attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa atatatttac
     6841 tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata taatccgttc
     6901 ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt catacatagg
     6961 aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat ctacttgaac
     7021 tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa tatcatcttt
     7081 aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta ataatgccat
     7141 ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca gcatatttag
     7201 gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt ctttcttcta
     7261 attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa tcatttaatt
     7321 ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg cttttaaaaa
     7381 tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga gaggaacgtc
     7441 gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct tcaaaattcg
     7501 tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt gcgtaccgtt
     7561 ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga accacattat
     7621 ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat tcttctaaag
     7681 gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac aagtattcat
     7741 taagttcttc agatccagga gagccagcca taatatataa atgtggactt acttgagtaa
     7801 tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca aagctagtaa
     7861 tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa tcaattagca
     7921 acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg tctgtagtct
     7981 taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta tctgatgttt
     8041 taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa atatatttag
     8101 tatacttcat atacttaata tattcgatat atctaatata ctccaaattg ataagaattc
     8161 agactttata aggaatatag tggatatact aaatatattt gctatattca gtatatcaga
     8221 tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag agtgaaaaga
     8281 ggtaaactta attctataga aaccacagat aattcactat ttacaaagta gcaaaagcag
     8341 tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat tgattttagc
     8401 agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt ctcaagaata
     8461 tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc cgattgcctt
     8521 tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt gtacttaaat
     8581 taaatattct taaacactga agagagaaca catactatgg ctgagattaa agctaaactg
     8641 tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat ttattcagct
     8701 attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa acctgcaggt
     8761 catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca attaatgaat
     8821 gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga aaagaaacta
     8881 acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta tcgaacagtg
     8941 gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt tgatatcgat
     9001 tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt gggctacatg
     9061 ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact aaagacacca
     9121 ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa aatttcaaaa
     9181 acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg taaccacttc
     9241 ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa ctatcagtat
     9301 tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa aacaaaagaa
     9361 gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca agtagatgaa
     9421 ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg acgtcttggt
     9481 cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat
     9541 acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca
     9601 gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa
     9661 ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc
     9721 attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat
     9781 aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag
     9841 ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca
     9901 cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga
     9961 ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa
    10021 ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt tggactcaac
    10081 gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa
    10141 gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccttatacaa
    10201 tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata
    10261 cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt
    10321 atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag
    10381 tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa
    10441 aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga
    10501 ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa
    10561 tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta tttacttaaa
    10621 aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa
    10681 gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac ctggcttcct
    10741 ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc attaggaaaa
    10801 ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat tggagtattt
    10861 ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga catttttaca
    10921 gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa
    10981 tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga tttaattgtt
    11041 gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag aacaacaaaa
    11101 ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat gtatttgttt
    11161 gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat taaacgttca
    11221 attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga gtacaatgtt
    11281 gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag tatccatgaa
    11341 tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa agcaatagat
    11401 cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata tggcatcgga
    11461 aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt ttggaacagt
    11521 agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg aaaatgattc
    11581 ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag aaggagcagt
    11641 tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt acgatgaaca
    11701 ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg ttgattcgaa
    11761 agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg atgaatggga
    11821 acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg taaagagcat
    11881 aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt
    11941 ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg atagtgaagg
    12001 atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc tatccttttt
    12061 aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag
    12121 aaattgatcg aagatgtgac gaa
//
LOCUS       DAZUKT010000086        11742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000086 DAZUKT010000000
VERSION     DAZUKT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.088"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..3783)
                     /locus_tag="ACG816_002400"
     CDS             complement(142..3783)
                     /locus_tag="ACG816_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921245.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGELLKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(3786..5735)
                     /locus_tag="ACG816_002401"
     CDS             complement(3786..5735)
                     /locus_tag="ACG816_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4921246.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(5777..6835)
                     /locus_tag="ACG816_002402"
     CDS             complement(5777..6835)
                     /locus_tag="ACG816_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS4921247.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(6954..7409)
                     /locus_tag="ACG816_002403"
     CDS             complement(6954..7409)
                     /locus_tag="ACG816_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921248.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(7993..8796)
                     /locus_tag="ACG816_002404"
     CDS             complement(7993..8796)
                     /locus_tag="ACG816_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4921249.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            9839..10999
                     /locus_tag="ACG816_002405"
     CDS             9839..10999
                     /locus_tag="ACG816_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4921250.1"
                     /translation="MKITEKNVNPKFEPTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLEDHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            11034..>11742
                     /locus_tag="ACG816_002406"
     CDS             11034..>11742
                     /locus_tag="ACG816_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4921251.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAV"
BASE COUNT         3640 a         2264 c         1719 g         4119 t
ORIGIN      
        1 aattatacaa ctttgaacag taaatattta attcatttat agacaattta aaataatttt
       61 tttgcataaa tagaaatttt ccttgacatc ttcagttaga aatcaaagta aaaagaagga
      121 gcaaatcaat gctccttctt tttactcgtc ttcagactta ttttcagttg atttttgatt
      181 accgtatttt acaatatagt ccttgactga catggtagat ttcttcacta ctttaccagt
      241 tttggatgct cctgtagcag tagccttggc aaccttagcc gttcctttac ctattttaat
      301 agcttcgttt ttagcttcat aggttatccc tgctagacta ccttttggag cttttgctaa
      361 agctttatct gctgaggtgt aaacattttt accagttttt gatagatatt caacggctga
      421 tttagctgtt gatggtttct tggttgattg attagatttc cctgaattat ctgaagtaga
      481 tttagaatta tttgttctat tatttttatt tgtcagttca ggttctctaa ttgattgatt
      541 gtctttcgac attcctgatc caatatttgt ttcatattta tctgtttgat taacattagg
      601 agtaggaatg aactttttct tcacatctga ttgttcttta tccgtttctt ttgttagcga
      661 ttctctgtct ttaagtgctg tgtcattccg aatagcacta ttatcaaagt tgctgttaag
      721 ctctcttgac tgactttcac cagatgctcc atctcctaat tcccgatgca ctctgtctga
      781 cacgtcctca ccactcattt cattcctcag ttcttgatga gtttgatcta aagtaatctc
      841 gtcatcttca ttctcaggaa ttgcaacagc aattggcgta tgtcgttgtt gttcgagttc
      901 tctatttcga tctgccacaa gcgcatctct cgtctcaatg atattcccat cctcatcgac
      961 taaatctcca ttcacattat atccatcgct gtgtttacca agtttattac caaactcatc
     1021 aacaagattt ccatcttcat caactaaacg ccctttttcg tcaactaaac gtccgttact
     1081 atcctgacca aaatcaccat ttagcaacct gcgtccattt tcgtcaacaa gatttccatc
     1141 ttcatcgact aagcgacctt tttcatcaat taaacgtcct tcgctatcca taccaatatt
     1201 accatcttca ctatcttcca atggtttacc attagtatct ataaggtttc catcttcatc
     1261 gaccaaacgc cctttttcgt caataaagtt gcctttagca tctttaccgt atttactatc
     1321 ttttaataat tcaccatttt catcgacaaa gtttccgttt tcatcaacca tacgtcctaa
     1381 atgatcaata tattgttggg agttattacg tagttgatct tgacgaacta aatctttgaa
     1441 agaggtatta ttgattgttg aaccaactcc gccaccgttg aatgacattc cttctggtgt
     1501 ttcaggaatt actttatcag caatggactt agcatcagca ttgctatctc ccatattgtt
     1561 caaacgttca gccatttttt gcgctccaac tgctccaaga cctcctgcaa ccatatcttt
     1621 tactgagctg ttgccaccat cataataggg atttttagct ccttggcttt tcttgattgc
     1681 tttagaaacg ccgtccacaa ctcgcacagc atcatttcta ttaccaaata ataacttagc
     1741 acgttcatca aacccaaacg ctacttcaag tggtaagcca ataagcatat taatcaaatt
     1801 atttactttt tcgccttttt taaagactaa aagatatcct acacctaaaa tcatagcaga
     1861 tcgaacaata tttgctacgc catcgccaac taatgtatta ggaatgaatc ccacagcagt
     1921 atcaccaaat gcacgaataa aagtaatcag aaagataggt aattgtagtc cgatcagaga
     1981 tgttgtaaca acacctaatg aaattgccat cacacctgct cctgatgcaa gtgatcctgt
     2041 tagaactact tcccaaatcc gaataaattc acgtttaaat gctttcacga tattcacatt
     2101 gaacatcgcc caagcacatg ctaagtaaag aactgctaaa acgattgatt cagcgttcat
     2161 cgctagccac gagccaaact tacccatagc tccatgacca actaaggatg ctgtacgcca
     2221 ttctgttttt actgttgaca tattcttagc actgccttga attcctgaag gagcgatatt
     2281 atacgcataa aacttagctc ccgatgtatc aagtgccgat tggagcaata aagctaccga
     2341 ctgtgtgcta aaagttgcat tgttagcaac gccccaaggt tgagtagcat caaagttatg
     2401 cgctgttcct tttccatcag gcttgcgatt gtcgtctgtc acattttgat ttgctgacca
     2461 aatatatcca tttaaattgg ttggtttctt aattgcactt ccttcttttc catcccctaa
     2521 attatagcct gtccaaacag caggcgctct tcccatataa gcatcatttt cggcgacatt
     2581 aacatcaccg gcataagtat taatattgaa agtatcattc gatgaccatt cttttaatag
     2641 agccataccg actgaattag aatctagatc attattttgt ctggcataac tgctagtatt
     2701 gatcgctctt atcattgcag attttttaga tggttcataa tctgtatcaa cgaacttgat
     2761 ttcggatgta ggctttccgt caggtacatc accaccagta tcagatgatg gagcaagatt
     2821 ttgagtagaa gcccaaagtc ttgtgttaat gatgtagtca gaaacaacat ttgttggtat
     2881 atctccatct ttttttgctg actggatgcc gttgatataa actcccaata gcgatcctgc
     2941 cactggtaag aatataaaca atacaaaaat acgaacaatc catttttcaa gtactggtga
     3001 taatcccttc gagttaacac catctttcga caataatttc attccttgat aacctagtat
     3061 tgctgtgaaa aagagcatag ataagcttgc tatcgcttta aataattcat tatttaatcc
     3121 caaattgtta agcaagctag ttaatgcttt agataccgga ttagatacat ccgatccacc
     3181 ttctccccac cccataagag aaaatatatt catactcatg aaaaattttc caaggagttc
     3241 atttatggat tcagcaatat tagaaaggat taaactaatt gatccgataa aactaccaat
     3301 cgtcctacca atacttccta gaatggatga tgcttgactg gcaatagcat ttcctgagac
     3361 tgtttcaata tatccgtagt aactataagt tgctaaagta gatgctagtt gagtacctgc
     3421 attaccccct gcaatcgaat taccttgttt agaaatgtca gaataaggga aatcggcact
     3481 gataccacct tgtcctaaca aattcatttg tccagtttta gcggcactat cattaacttc
     3541 tttatctccc attaggatac gagcatatag tgtccacatt ccttgttctg ccggtttact
     3601 ttccgcatat gcaacgttct tatttataac tgtttcttgt ttgctatctt gattgtcagg
     3661 agttggatca ttcattccaa aaggagcgcc taaagtaact gctaatactc ctccaataac
     3721 tagacctctt acgacccatc gttttattct aaggttttgt agtaaatatt tcttgaattt
     3781 catagctacg cccctccaat cgaaaataca atcttgtttt ctttactttc agcaacggaa
     3841 ggctttgcaa taatttcagc attagaatca ccttttcctt caaacgctcc ggcttcactc
     3901 aacttaacat cgaatatttt tgcgtttgga taagtaatta ttgcttcagt aatcgcttct
     3961 tcaaactcag attcaactaa atgcaagata ttccttgcac cccctgattt agaatcctct
     4021 tgtgaagcca tgtcaaaaac taagtgcatg gagagatcgt ctgcataaaa attttgacca
     4081 tatactcttt ctttcctagg tggaagacgt aaatctatat ttaaatgttt tcgtactaat
     4141 tcagcactct tcaacatttc attataagca atttccagaa gagtttgttc ggataaagcc
     4201 caaaatggaa ctattcgtcc aaatcggtta ataacttcag gctttagacc ttcaccaatc
     4261 aaggcttgtt tgatccctga tgcgactttt cggtattctc tttttagttc agtgctgtat
     4321 tcattagaat tttccttgtt tgtaacacga gatagtgagc tatatcggtt taacgaatcg
     4381 aacatctccg cacctgcatt tgttgtcgca aataaaattg tatttgacaa tgaaacggtg
     4441 tcgtgaactt ctcgatcatc ccatcctctt tcagtaaaac ttatcctacc ttcatctaaa
     4501 attgataaaa aggcatcgag tgtttctaca ccagctttct catattcatc acacacaata
     4561 atcgcagaag gatcatgcct tacatgagta cctataagtc gtctaaaacg cattgcatca
     4621 tctttggttt tgtagtccgg catattaact aagagaactt tattttcatc gccataaagt
     4681 cctcttgcca cggctttagc cattgcagtt ttaccgaccc ctgttgatcc tgtaaacaga
     4741 gcagttgctt ttggttgctt tcgaccttta aaacgatcaa atccaagctt catacgttta
     4801 aacaacattg ttatagtata aactgctaac ggttgtcctt ttatggactt taattcctct
     4861 tgaagttttt ccatatcaaa atcagaatct agctgaatat tatacttacc aaatatttgc
     4921 aatagacttt ctcgattata agcatttccg tctactcgat cttttgcaat caaatcctcc
     4981 attacagcaa ttgatttagc cggttcggac aaatcctcac gataaagttt gtttgcttgt
     5041 acgatttctt gaagtaattc atcaggaatc ctttgcgata atctaaaaga agcctgagga
     5101 tcatctttaa cagaggatgt atttagccat tgtctcaaaa tatcgacgac aacatcgctt
     5161 tttagttcat tcaattctaa cggttgtagc cgtctcccta aggcatcatc tcttttgata
     5221 tagttttcat attcttttct tgtagtagcg gtaatcattt gaagtgattc atttgctaac
     5281 ggttctttta gcgaatcacc cccaatttta ttgccactcc caaaagagct aacaacttta
     5341 tgaaactcgt caatgaagag gacaaattca ttatctttgt cctcagcttt taaaattcgc
     5401 tcatattctt tcaatcttgg aattaatgtt tctaaacgtg tttggaggac atcatctccc
     5461 ccaacacgca attgtccgat tgataattta tatacttgta ctttctttcc atcttgtcgc
     5521 tgtctttcag cccattcact tgcaagtgct gttttacctg ttccggcttc acctaacaag
     5581 atagcttgcg gtgtttccgg tctattcaag atcagtgaca actcatggag ttcgtcatca
     5641 cgtcccacta acttatgttt tggtggtttc attggtgtga gagcatgatt aaattcggtt
     5701 tgaatattct taaaaaactc ggtactattt tccatctgtt tgtgttcact tcctctaaaa
     5761 taagaataat ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc ttcatgtctc
     5821 caaaagaatc tttgttaaag tcagcatttt tgtattgatc aatcaatttt tgtgtatcat
     5881 tgattgcttt aatttcggtc tccattcgac tgtctagttg acgaatactt tcttgcgaat
     5941 ctttatcgtc aggattttgt tgtaaacgtt ccagatactc agcttttgtg gcttcaattt
     6001 tcttgatgtt agtatttgaa gcatttaact gatcttctag cttatttaca acattattct
     6061 tacttgtaac tgctacaact tttgaataat caatatcgcc atctttggta agaaagcttc
     6121 cttgatagac attagttgaa tctgaatatg gatttattct gaaattgata ttatcaggag
     6181 tattaacagg ttttgctttt ttcccaaaag aaattactcc ttcattatta acatcattca
     6241 tagaatattg agaaagtgct tttgtaactg aatctgcttc attcacattg actttggtat
     6301 catcgtcagc tgtatcatca ccagaaatta gtgatacttg gtttgcaata aaaacttgat
     6361 aagctctttc ctctaattta ccttttagga ataagaaagc atccccatca gtacttaaca
     6421 tcccatagct catttcaagc ttcttaggac gatctttttc ttttgttgcc atatataagt
     6481 tatagttttt tccttgcgta gataacaact ttctagcgtt ttgggagtaa ccaagtttta
     6541 ctactgttac atctctattt tgatctgtcc acacatctga aatagtgatt tctgctcctg
     6601 atcgagcaaa ctccaatgaa gaccctatag gtgttgtatt gttcacgaga taataattat
     6661 agtttcctaa ttgataaatg tagctaccca agcctgttaa taaaaaaagc actcctagaa
     6721 aagttgcaag cataatgcgt aatccgtgac gtgttctctt atccttatca ccaagcaacc
     6781 ctccaataat actgtattga tctttggtag atgttgaatt gacgtttttt tccatcagtt
     6841 tgtctgcccc ctttcataaa agagcaaaca atcagacgtt ttttcggtaa ttctaggttc
     6901 gggattttga atcatttcgg tatattcttc cccattaata tttaacacaa aaattatctt
     6961 cctttcttcg ttcctttctt ttgctcttta ttttccaaat aatcaatgat ttttgctatt
     7021 gtaccaagat agtaattccc ctcagctttg ttttttcgga ttttgctaac cattggagta
     7081 ggtacaatct cttcatctct caattcactg aaagattttt tacctagata ttttccaata
     7141 acagatttta aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc ttttagttca
     7201 tggttcatat tgtcaacgac ttttgcggtt tcaataattg ttttagttct cagcttaata
     7261 atttcatctg gaattttcaa tcctcgtaat ttcatcgttg tactaactgg taacccatgc
     7321 tttactgcat cagcaatact aaaacttttt agatcatcta gtaagctctc catatagtta
     7381 acaaattcat gccaattaat agcattcatt gtctcacccc ttcatttttt taaaatgagg
     7441 ttcgggcgta acacatttat gttattataa tatcataata acataaataa atcaatcagc
     7501 aaatttagat atcccttaaa gtatcagcaa ttattcaaca agatatttat tttagctatt
     7561 tcagtcatca taaaagacta attagcaata aaataactaa tattatccat ttatcagacg
     7621 aaaagaaaaa acttatctaa ttactctata ttaactttta acaaatagtt gacttgtcca
     7681 aaaatgttac cttcaatctc acaaagatat taagatgttt cctaccaaaa ctgatatcac
     7741 cggacttttt attctgtgtt gtccaaaagt gttaccttta gtcgatcaac aaattaaaaa
     7801 aaattgtttt tttcttgact tgtccaaaag tgttacccat agttgttaaa atttgtatag
     7861 attagccatt ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa acttcttgca
     7921 tttattgtcc aaaagtgtta cccatacttt tttaaagaag caggttgtat taaaattaca
     7981 acctgcttct tctcaatctt cttctaacca attatgcatt gaaatggttg gcaagtttga
     8041 ttctggctca ctaaaagtga atctgagccg atcaactctt cttcctttct ttgcatagat
     8101 tttttcataa tcaaaagttt caaatacctg tattccgttt tcatcaggag ctaaaaattc
     8161 ttctttcgcc tgctctagta ttcttttgtc aatatttgtc attctataac ttgaaggtat
     8221 acttaatgct cttctaaaat cttcaacttt gactacccaa taaccatttc gccactcttt
     8281 atttctgtat tgcatgagat gacgatacaa ttcttttgtg tagctacttc ttagttttgt
     8341 aaaattatca agttcaaaac gagtaaagtt tttgccaatt gagtttaaga taaattcaaa
     8401 ttcctcattt gccttaattg taactgtttc cttctgttta tctatccgga atttttgaaa
     8461 aagccacagc tgttcataga cgtctgaatc ttcaaaacgg taggttaaag tacctagttt
     8521 gtcactcaaa gcttttaaca cagaataaaa actgtccttt tctcttctgt cgtatttgac
     8581 caaataacga aagaggttga aatcaaaagt tacttctgct gttccttgac gtttcatttt
     8641 tgataaaata gcccaaaaca catccatttc aacaggctta aatcctcgca aagctgttgt
     8701 attcagacca ttgttataac gcacaattgc atttgtttgt tctgatacta aatctacatc
     8761 actatcaata atcttttcgg taacttcttg agacattgat tactctccaa tcttcttttt
     8821 gcgctccttt aatcatgtta atctggtact aattaaagga aacaattttt tctgcttgtt
     8881 acctaaatta aatcacaact aaaggtaaca gtcaacaaag atttgttact aatctaattt
     8941 tctatttttt cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc tagtgtgata
     9001 cctcaaatgt ccacttttgt tacctttgct gtccaaaagt gttaccctta ctgtccactt
     9061 ttgttacctt tactgtccaa aagtgttacc tttatacccc tttaatactt actctcccaa
     9121 gagattaagc ctcccctaac agatatatta acagatattt aacagatata ttaacagata
     9181 tttaataaag gaggattact cctctcttat ttcaaaatct taaaaataaa aaatcttaaa
     9241 aaaaactaac actatctagg agtttatttt gcttgtgtaa ctttgaataa ttgaaaaaaa
     9301 ggaaaaggag gaagcttcag cttccccttt tatttatatt tataatatat attttaatct
     9361 ttaaaacttt agcaaccttc aaaaaggctt tatatcaatc ttaaatagat tttagggtgg
     9421 acataaagcg atgcgaacat ggacaaaaag cgatgtgaat gtggacaaaa agcgatgcaa
     9481 acgtggacaa aaagcgatgt ggtattccga atatatccaa aaaaagaaaa agtatgtgaa
     9541 tttatttcta aaaactagaa aaatatttgt tttttaattt tggtaatttt tataatttcg
     9601 tatttttttg aaaaggattt ttatattgaa aatgttctaa aatagcgatt tctgctgtct
     9661 tatcaatgaa aattgattaa aaaaataaaa tgtgttttac agagattgag ttatgttata
     9721 aatttacgat acatttctta ttgtatcgta tcgaaaacag gtctatactt attcaatgga
     9781 atcgtaccag ttccaaattt ttaagctcgt accaagctta gaaagaagag gcaatcgtat
     9841 gaaaataaca gaaaagaatg taaacccgaa atttgaacct accgaaagca atctaaaaaa
     9901 gtttgattta actgttactt tatcgaatca acttgtagat agcattgttg aaatggatgc
     9961 tatgccacaa aaaatttatc tttatgctat aggacatctt gaccctaaaa atccgccttt
    10021 taataatact gttaaagtat ctaaaaaggc tttatttgcg ttgatgtctt ctagttcatc
    10081 ttcttcaaaa cattcaagat taaaacaagt tattgaaaaa ttgcatcagc aatctgtcat
    10141 taaaattcaa agaactaaac ctaacggtaa gactgaatac aaagtcatct caccgctaga
    10201 aattactgaa ttcaatgatt tttctgatgc agtatctatt aaatttagta gttcaattat
    10261 gcctttatta acgaacttag aagatcattt tacgcaatat acatttgatc aagtagttaa
    10321 cttgacaagc aagtatgcga tcagtttttt tcaattattc actaaagatt ttaatctgta
    10381 tgaagcaaat agagataact taacatggag tacaaaatca cttgatagat taaaaaatcc
    10441 tgttttctcc attgcagatt tacgaaagaa atttagtatt gagaaaaaat ataaagcttt
    10501 tcgagacttt gaaagacgtg taattaaaga accgattgcc caactgaata aatacactga
    10561 ttttaaaatt gactatgagc ttatcaagaa gggtcatact tatacaaaca taaagttctt
    10621 gcttacacgt accatgtcag ttccggtacc ttataagcaa gaacaaagag accctgctta
    10681 tttaaaaggc gcagaagata aaacagagaa aatgaatcaa tcgtacttga aagctatgaa
    10741 tagcaaatac actaaattac taatcaaaaa ctttttgatc agttctgccg acttatgcga
    10801 tcttaaactt atgagccgac ttcagaataa agtttatccg ctttatgatc gtttatccaa
    10861 agatagagga tttgaatcag ttgtcaatca cctatcttac gttgctactc ataaggagga
    10921 ttacacaata aaaaatactg ttaaatatct cgaaaaatca attacgagtt atttgcctta
    10981 tgtgaacctt tatgattaac aactgaaaaa atgcgtgaag gaaggaaaaa cttttgaaaa
    11041 aagatgaact gacaaagtac aatgaggagt tcttagcaac aattgacatg aaaatggatt
    11101 taaagtcgta tgaactttat caattgctgt tcagttatat ctcacccaca aagctaaaga
    11161 aaacaggcgt agtagattta cctgttaaag cgattattaa ttacatggat gttgatcaaa
    11221 atcttgaaac ggaactaaaa accatagtta acactttgca aagacaagca gtttatgaaa
    11281 tttcgaatct agacaaaaaa tataagacgt atactactgt atctgctatt gatcggattc
    11341 attgggattt agaagagcat agtgtatctt tcagtttttc agaagatgta attcccttaa
    11401 ttgtctcaat caaaaagaag tatccgttct ttgtttttga agacttagca tcttttcaaa
    11461 aaaaatatag catcgtgtta tacaaatatt tggcgatgta ttataaaaaa tatcttatcg
    11521 ccaaaaataa aggagatttt gagaaagctg aaatatataa gaatcccctg attgaaatag
    11581 ttaaaattcg tgagattacg aaaacagaaa atgagtataa aacaatatcg aattttgata
    11641 gacgtgtttt aagggaatca gttgaggaaa ttaatgaaaa atcaatgctg aatattgatt
    11701 ttgctaaagt tagaaaaggc agaaattaca attttgctgt cc
//
LOCUS       DAZUKT010000087        11597 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000087 DAZUKT010000000
VERSION     DAZUKT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.089"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..1205
                     /locus_tag="ACG816_002407"
     CDS             240..1205
                     /locus_tag="ACG816_002407"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4921252.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1304..2476)
                     /locus_tag="ACG816_002408"
     CDS             complement(1304..2476)
                     /locus_tag="ACG816_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921253.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2463..3608)
                     /locus_tag="ACG816_002409"
     CDS             complement(2463..3608)
                     /locus_tag="ACG816_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921254.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3622..4554)
                     /locus_tag="ACG816_002410"
     CDS             complement(3622..4554)
                     /locus_tag="ACG816_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921255.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4671..5750
                     /locus_tag="ACG816_002411"
     CDS             4671..5750
                     /locus_tag="ACG816_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4921256.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5747..6365
                     /locus_tag="ACG816_002412"
                     /pseudo
     CDS             5747..6365
                     /locus_tag="ACG816_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6475..6702)
                     /locus_tag="ACG816_002413"
     CDS             complement(6475..6702)
                     /locus_tag="ACG816_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4921257.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6805..9393)
                     /locus_tag="ACG816_002414"
     CDS             complement(6805..9393)
                     /locus_tag="ACG816_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4921258.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9413..10072)
                     /locus_tag="ACG816_002415"
     CDS             complement(9413..10072)
                     /locus_tag="ACG816_002415"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4921259.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10121..10624)
                     /gene="trmL"
                     /locus_tag="ACG816_002416"
     CDS             complement(10121..10624)
                     /gene="trmL"
                     /locus_tag="ACG816_002416"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4921260.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10845..>11597)
                     /locus_tag="ACG816_002417"
     CDS             complement(10845..>11597)
                     /locus_tag="ACG816_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4921261.1"
                     /translation="NNHRFDLSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPV
                     IETIAQHLPEEVSLLDVGCGEGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAF
                     WCVADLTNLPFADQTFNAVLNIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAF
                     YPNQPEKQSYSNERVVAKFAEAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEER
                     IKADLYKNPLKKITIDIELLKGTKRKRFSSSF"
BASE COUNT         3412 a         2470 c         2030 g         3685 t
ORIGIN      
        1 ttctccttac gtaagtaatg gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt
       61 cggacctttt tcattttcct agacttctgc tataataacc aagaattaat tttgtaacag
      121 atttttttga aaaaacaagt ttctttcatt caaaaaatag aaaaagcttg ctaattattg
      181 gttaaccagc aaacaggagt attaaaatat cttgttttat tgaatagcag gaggaaatca
      241 tgaaattcca ttatcatata ttgatcaatc catcggcagg cagcggaaat ggctataaag
      301 tagcagaaag aattttaccg gtattaaaaa ataagcatat tgactataca cttcattata
      361 gtgaatacaa aggacacgat gctgagattg ctgaaacttt ggcaaaagaa acattgattc
      421 cttggataga agaagaacag aaaacaatcg atactttccc ccttttgatc atcgtaggag
      481 gtgacggcac cttacatcag gtacttgata ctttctatca gatggaagta gaattcccag
      541 ttgcttatat tccagcaggt tctggaaatg attttgctcg aggggcaggc cttccaaaaa
      601 atcccaaaaa aggacttcag ctgattttag cagctcaatc accagaaaaa gtccacataa
      661 tggcttatga agaaaaaata agtgaaaaaa aaggaatagc tgttaataat ttcggtatag
      721 gattagatgc agctatcgtc catgcgacaa atcattcttc cacaaaaaag cgattgaaca
      781 aatacaactt aggatcattt tcgtatttat tctctattct ccgtgcttta ttcactcaaa
      841 aaggatttcc aattctggta gatgcaggag gaaaacaatt aagtttttca aacgcatttt
      901 tatgtacagc aacgaagcat cctttttttg gcggaggaat cgcgattgtt ccaactgctg
      961 atgcgtctaa accagtcatt gattttgtcg tggtcgaacg aatcaatttc tttaaaatct
     1021 tgtggctcat tcttttactt atacagaaaa aacaattgaa atcgaaatat ttccatcact
     1081 acacaacaaa cagattacgt atcgtttcaa caacaccgca acatggtcaa gaagatggag
     1141 aagaaatgga aaaacgacct tttgatatca caataaccac aactaaacag ttgttttggt
     1201 gttaataaaa gaggttttgt cagaagctac atctggaaca ccgtttattc ggaaaaaagg
     1261 actgtgacac gacttttgtc acagtccttt ttttactatg cagttaattc tttgcccaat
     1321 tgggttgcca tcgatagttt ctttctttac ccaaaataaa ttggatcatt gaaaacaccg
     1381 cagcaaaagc aactaagaca caaccattga accaaaaaga ttctgcaaat gtttgattgt
     1441 aactgagtgt ttcactgatc aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg
     1501 ccgctatttt gctaaccatt tctggcaagt attctgctgg tacaaacacc ccaccgataa
     1561 agcaactgcc tgtgataaaa atatctttga tcccaccgct ggtttctgag ttttttacca
     1621 atgaagtgat acaagccgaa aagctaatga tcggcaataa aaacaagaaa gtatttaaaa
     1681 tatgatacca taccgttata tcccacgagg tgtggatcac taacagaacc attagaagta
     1741 agaagccact gaacaacagc agcgagtaac taatcaaact aaaggttact ttgcgattca
     1801 agtttcgttt ggtaatgggc gcactctggt ttcgtttact gatctctttg cgattaaacg
     1861 ctagattgat gacgctgaaa ccgctaaaaa tggatagaaa caacccataa gacaagaaat
     1921 taaaggttgt gcccgtcaac agctttttca tccgctgact atatcccgca cttagctcaa
     1981 cttctccagt aaccgagagt gccatttgtg tctgttccat agctgcatcg attgttttat
     2041 cttcttcttg aaacagaatc agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg
     2101 ttttagaaaa ggcatctggg cgactctgta tcttgatcgt cgccatcttt cctacttcga
     2161 cttgttcggt aaagtctgca ggcagatgta agataatgtt tacacgatta aaataaagag
     2221 catcatcgat ttctttttgt gaggttccat ccaatgtaac gactgtttcc ttgtcattca
     2281 aatatttacg aaggccatcg actacaggcg actcttcttc actaatcaag gcgattttcg
     2341 catcttgcag agaggtattt tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa
     2401 cagtgataat tacacctatc agtaaattcg atttatttgc tttaagcaat gtccaaatcg
     2461 ttttataaag cgtcataacg cacccctctt tccatccaag catttcctaa cgcaaaaagg
     2521 aaaataaatc cgatcaacca tagaagattg gtataaaaac tagccaaatc agtgtagaaa
     2581 taaagtttat acaaactttc gctgatcaag ttgacgagat ttaattgacc aacaatcgga
     2641 atatgaagat caatccaata cttcacttgt tccgacccca tcataccagc aaaaaagctc
     2701 atcgcaatcg tcactgaaat caagatgcta tccttttgca ccagattcat ttttggtatc
     2761 aaattaccaa ccaatgtacc taacaaaatc gcacacaacg accccagagc ggtaacaatc
     2821 atcaaccatt gccaacgttc accaaagtct acttgataga taaaacgaaa gacagctaac
     2881 ataatataaa tctctgtcaa aacaatcgta aagcttgctg ccaaattagc gattgaaact
     2941 aacgattgct ttctaggaat caaggacaag cgaataccat ttgacgattg atttgcttgc
     3001 tgatcctgta cattgcggac cccccacatc gtcccgtaga gaatcgccat tccaatcaga
     3061 gtgaagaaat aaaaactttt gaaactcata ttacgacctg catgaacttc ttgaaaagat
     3121 acttcttgtt caaaaacagt catgatgggc gcgagatcaa cgttctccac ttctgccact
     3181 ttactcgaaa ttataccaat acgctgtaaa aattgatcca tgacgttttt catcaacgtc
     3241 tgactcattc ctgcttgacc cacatggagc gaaacagatg aaccatcaat gagataaaaa
     3301 ccagccaaat catctgcatt tagcttttca ttcgcttcta cttccgatag ttcctgtact
     3361 tcaaataatg gtttatcatt gacttcgact tgttctaaga ctgtggtaaa attttccgaa
     3421 agcgctgttt gatccgcttt gattacaatt ccagtattaa tcgtttcaag attgccacct
     3481 tgatcaatct cgccaaaggc cgccataaac atcaatccta aaagaattgg aaaggccaat
     3541 gtccaaaaca acatggattt atttcgcagc agtatttttg tgcgatattt ataaaaatgc
     3601 caaaacatta tttcccctcc tctagtctct caaatccttg ccagtaatct ctaaaaagac
     3661 atcattcaag gacgcttctt tggttgctat ttttccgtaa ctgatctttt cttcttgtaa
     3721 gattgtaaga atcggcaaca atactgtatt tagttcagta gttgttactt ggagctgacc
     3781 ttttttataa gcgattccat cagaagctat tgttcgctga atcgctgaca acacattttc
     3841 tgggagtaat gggatgtcga tcgtcaaacg atccgccgtc cgtaccatat cttttagaga
     3901 ttctttggtt cctttagcaa tcacctgtcc ttgatccatg atcacgatct ggtcgcaaag
     3961 aatttctact tcttccatgt aatgtgttgt atagacaatg gttgctcctt gtcgattcag
     4021 ctccttgata ctttctaaaa ttttattccg actttggggg tctacagcta ctgtcggctc
     4081 atctaaaaaa attaaccgcg gcttgtgggc aatcccacat gcgatgttca accgacgttt
     4141 caagccacca ctaagttctc ctggacgaaa cgaaacatat ttttctaatc ccactaattg
     4201 aatcacctct tcaatcaagg ctttcctttt ctctttttcg gaaacatata aaccgcaaaa
     4261 ataatcgata ttttcttgca cacttagttc gtcaaagacc gcaacgtctt gaggaacgac
     4321 cccaatcatt gccttgacag cataattttc aggagtcatt gttgtcccga acaatttaat
     4381 ggttcctttg tcataattta acaaagaaag catacagttg atggcagtcg atttcccact
     4441 gccgtttgga cctaacagac ctaagacttc tccttcttgt acttgcagat caaaatgatt
     4501 caatgctaca aaatctccat agcgtttgac taatttatca atttcgataa tcatcatgaa
     4561 aaactccttc caaatttctt gttgatctga ctataataaa gtgcaagaaa agaaactagt
     4621 gacgattggc taaaccacac atgacatttg tcacaatgaa ggagtctgct atgcataaat
     4681 ctcggctact caacgaaact agctttctac tggttatctc attgcttttg ctggtaagtc
     4741 aaggattcgt tgctatgcaa gttatctatt tattgatcgc cgttttattc actggtgtca
     4801 gttatttact gcctacaaag ccaaattgga ttttccaagt tattctattt cttttatgtg
     4861 cctttttacc tgatcttgtc ctgtttctgc cagcgacgat gcgagtgatc gcccctgaag
     4921 aaagccacct tgattacttg tttttgttag gttttttgtt gatttcgacg gttggtaatc
     4981 atccccccat caaggtcgcc tttgtcgtaa ttttgctgtt tttttccttt tacttacgaa
     5041 aaaaagatca acgaacgacc gatcttgaga aaaagtacat tgtctccaaa gatactcatt
     5101 gggaaaaaca acaagagtta tcggaacaaa ataaagcatt ggccctgtca caagaaacac
     5161 aattagcctt gcaaattgtg gaagaacgga accgaattgc ccgagacatc catgacaatg
     5221 tcggtcatct gctgtcaagt gcgattttac aaattggtgc gcttgaaatg atcaatcaag
     5281 aaccaaaact aaaagaacca ttagaaaatt taagtgccac cgttcacact gggatggatc
     5341 ggattcgtca aagtgtccat gacctccatg agacatcgat ctcttttcaa cactccttag
     5401 agtttctgat cgctgatttt ccttcttctg ttacgattga aggaaacttg tttgaaggat
     5461 tgaatgaaac gcagcaaaac tgcttcttaa tggtcatcaa agaagcctta accaacagta
     5521 tcaaacatag tcatgccaaa accgttgttt tacattttcg aacgttgccg gccttttatc
     5581 gcttgcaaat cacgaatgat ggcaccagcc cacaaaaaaa ttctgctccc ggtattggtt
     5641 tgaaaacgat gcgtcaacgt gttcaagaac aaaagggaca agtccatatt ttccaagaaa
     5701 accagcaatt tcagttaacg atcattttac caagggaggg acaccaatga taacagtagt
     5761 cattattgat gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa
     5821 agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgcgaaacc catcgtcccg
     5881 atgttttgct gacggacatc cgcatgccta aacaaacggg catcgatgct gccaaagaaa
     5941 ttttggctaa atttcccaaa gccatccttt tgcttttgac gacattcaaa gatgaagaat
     6001 acatccgcga agcattttcc atcggtgtca aagggtattt aatcaaacag aacttgcaag
     6061 cgattattcc ttctgtcaaa tccgcttata atggacaggc cgtatttggt aatgaaatca
     6121 tcgagacttt cactcaaatg atgaagaata aaccgacgat tgatagtcat gcttatgctt
     6181 cttttagtga acgtgagctt gcaatcatca aagaagtagc tgccgggaaa aacaataaag
     6241 aaattgcaga tgccttgtac ttgagtgacg gcactgttcg caattacatc agccaattac
     6301 tagaaaagct agatttacgg gatcgcacgc aattggctat ctatttttat cagcatttgc
     6361 aataagtctt atctaacaca aaaaaacctg aatgatgctc cttcgcatca ttcaggtttt
     6421 tttcacatct tctattcaat tgtcccgcaa accatttcct cacgattgaa agaatcaagg
     6481 attcgcaggg ctgccaggat tcgttggatt gttcggcgtg ttaggcggaa aaataggcgc
     6541 cttgtctaac gttttttcta gttttgattc ttcacgatcg tctcgttcta catcaggact
     6601 ttcattcaat ttgaattctt tttccatctc atctaacttt ttaaagtcat tgccagattc
     6661 ttttgctaat ttcgctaatt tatcttcggt ttctttagcc ataaagtaac gccccccttt
     6721 tgatttcagc ttagcatatc acactgaaaa aacaaaagaa aacgctaacc aacgatcatt
     6781 tttagctatt tctcatcttt aaaatcaagc attttttgcc ggtataaaat cgtagggaga
     6841 actgttttcc ataccaatca tcggatctac taactggtta tcgatcactt ctgctgtaag
     6901 aacgaaatct ttagaaacag caggttctac ctcttctttc ttttcttctc caacaaatga
     6961 agaaaacaga tctggttcca ctgtttccat tacttggttt ttcgtttcat tcgtcccact
     7021 gtcatcttct acaggtagct tttcttttac aacccgtggc tcttttgtat agttcccatc
     7081 ttcaaatgga tcatttccga ccaaagaatc ttcataagct gctagtttgg tccggagagt
     7141 gatattcatt ttatcattat ccatgatcct tgatttcaac gtagtcatcc ggcttgctga
     7201 ttcattcgct acacaagtga cataggcttg ctcttcccct aacaaaatca acagttcctt
     7261 actgcgagtc actgccgtgt agagcaaatt acgctgaagc atccgctgat attgatggac
     7321 catgggcaaa ataaccatct tgaattcact gccttgtgat ttatggattg agcaacaata
     7381 agaaagagtg attttattcc attcgtttct tttataagtg acctcatttg catcaaactg
     7441 gataactaat tcatccactt tatcttcaga atcttttgcc aagatgatac ccgtgatttc
     7501 tcccatatct ccattgaata cattcaattc gggagtattg actaattgga ggactttgtc
     7561 tcctatccga taaacggtgt cgttccaatg tacttccttt tttccaggtt tccctggatt
     7621 gaatatctcc tgcatcattt tatttaacgc atcgatccct gcaggtcccc gatacattgg
     7681 tgctaatact tggatatctt gtgcacggaa tcccttattt ttcgcttttt ccacaatttt
     7741 agagatcaag ggttcaattt gataagcatc acttcggaaa taggaacgat ctttttgatt
     7801 ctttgtgaaa tcttttggca gacgtccttc tttgatctca tgggcaagag gaataatact
     7861 agaaccatca ccttgccgat aaatctgatt gagttccatt ttgggaattt gttcgatttc
     7921 caaaagatca tggagtactt gaccaggacc gacagaggga agctgatctt tgtctcctac
     7981 taagataact tgcatattcg tcggaatcgc tttgaataat gtgttagcca gccatgtatc
     8041 gaccatagac atctcatcaa caatcaaaag tccgccttct aattctttcg tactgactga
     8101 aggtgttttt tcccgtccat tcagccccag aagacgatgg atcgtactgg caggcagacc
     8161 tgtcgtttcg ttcattcgtt tagctgctcg gccagttggt gctgcaagta gaataggaaa
     8221 aatttcttgg gtatattttg aaggatccaa ttccagtccg ttcaactctg caaaaagatt
     8281 gacgattccg ttgatcacag tagttttccc cgtacctggt cctcctgtta aaataaatag
     8341 aggggaacgg attgcttcag cgatagcttg ttcttgcgaa ggtccatact gtatgccgaa
     8401 tattttttcg atacgccgaa tattcttctg cacttctttt tcaggataat ggatttcttt
     8461 tttacgactg agaaggcgct ggatcgaatt accgatcccc cattcagaaa agtacaaact
     8521 gttttcgtaa agattagtct cttcttgttg tatctttcct tcttcaacaa gttggataat
     8581 tacagtagca acctgatcag gctggatttc aattgggcga cttgtttcta aaatacggat
     8641 cgtttgctga agcaatgtct ccgccggtac gtatgtatct cctgtttgaa ctgcatgctg
     8701 aaaaatctga tgcaagatag ctgctcgaat tcttctcggt gaatctgctg caataccgat
     8761 ttgttcggct agattatctg cacgtttgaa cccgattcct tctatgtctt cgactagctg
     8821 gtacggattt tcttcgatga tatccaaaga ctcgttctta taggcctggt agatactgaa
     8881 agctaactga ctaccgaacc cgtaacgatt cagtcccatg atcacttgat ccatcccgtg
     8941 gtttagccga atcgtctcag caagcatttc tctttttggc ttcgtcaaac cggagatttg
     9001 ttttaatacc gaaggatcag caatgatttt ttcgatagca tcttctccca gcaattctac
     9061 aattttttct gctgttttct tcccgattcc tgggaatttt tcgctggaaa gatacgctac
     9121 gatcccattt gcagttgttg gcttttcttg ctcatatctt tcgagtttaa gctgccttcc
     9181 gtatttagga tgatccacta attctccata aaaacgataa gtctcatttt cttgaatatc
     9241 gccgaaactt ccggtgacta caatctcttt ttcacggtaa tcagctgtgg tttcactgat
     9301 gtgtaccaaa agcactttgt aaaaattact aggattttga aaaaaaatcg cggcaactgt
     9361 accaacgaaa taaggcattt cttctggctg catcggaagc cgcctccttc ttctatagta
     9421 aaggttctgg tgctgtttca gttcctaaaa aatctgtttg gttccatact ttcagttcat
     9481 atggcaataa tctgcaaggc tcttcagtct ctaaaatcgt caaactacta ttgaacaatc
     9541 cacccatttc acgcaactca cttagctctt ttcctgtcat ccattgaatg gctgcagtta
     9601 aagaagctcc atgtcctacg aataaaagtg ggccatcttt atgtcgggca accgcatctg
     9661 ctacaacagt ttcgatacgt gtgattgccg attcaatcgt ttctccgtga aaaggagaag
     9721 gatcatacag atctaaatga ttacgcaaat tcggcagatt atccggatat ttctggtaca
     9781 tttcatcgat cgactgacct tctaaagtac ccagccctag ttctcttagc ttatctgtat
     9841 aaaaaatctc taccggacga gcaagctctt tgttgatacc ttctgctgta tcacgtgctc
     9901 gctttgctgt actggaataa attgctttaa aaggaacata ttttaccttt ttcccaaatg
     9961 ctttgatttc ttcgtaactt tgtggcaaaa gcggagaatc gccattgctc ccttgaaaac
    10021 gtctttcctg attccattgt gtcttcccat gtcgggtaaa atatagttcc atacgcagca
    10081 aactcctgtc tttcttttat tttactctat aataaactgt ctaatccagc ttgtcatttt
    10141 catagtgatg cgtcaattct aaattaggga atccttgctg gcgaagtgct tcatacacta
    10201 tcattgcagc tgtattcgat aaattcaatg agcggacatg ctcatcattc atagggatgc
    10261 gtaaacactt ttcttcgttt tcacgcataa atgtttctgg caaaccggtt gtttcttttc
    10321 caaataaaaa gaagtgctct tctccatcag caaaattttc gtcactatat actttatggg
    10381 caaattttga gatcaaatgt agtttacgat tacctaaaaa tgttaaaaaa gcttctaaat
    10441 tactgtggta agtaatattt acatcattcc aataatccaa ccctgcccgt tttagatgtt
    10501 tgtcatcagt tgaaaaacct agtggctcga ttaaatgaag cggagtattc gtggctgcac
    10561 acgtacgggc aatatttcca gtgtttgcag ggattaatgg ttcaaataaa acgatatgat
    10621 tcatcaggat cattcctctc atatttatgt tcgtttctat tttctgacaa tttgattttt
    10681 gcataaaaaa aaacgtatca actcggtgtc agtcactgcg agtcgttacg ttagtgaagc
    10741 tcttctcact aacttttatc agaacaggaa cctgataaaa accaatgaaa aaacaacttg
    10801 ttgctaatat aacaccctca actttttttt gcaacctcta ctcattaaaa actgctagaa
    10861 aaacgttttc tcttagttcc ttttaataat tctatgtcaa ttgtaatctt cttcaaagga
    10921 ttcttatata agtctgcttt gattcgctct tctacctgcc attctaaagg tgacatggcc
    10981 aaaagatctt tttgattttc ctctggtatc tcaaattcat aggttacacg ctgacgttca
    11041 gttaatttca ttgcttcagc aaacttggct actacacgtt cattggaata actctgtttt
    11101 tcaggttgat tcggataaaa agcctgacgt agttccttta aataattttc ttgtggcacc
    11161 accttcaaca cttctccatc ttctgagagt acacgttgga attcttggta atgagaaggc
    11221 gaaaagatat ttaaaaccgc gttgaatgtt tgatcagcaa acggcaaatt ggtcaaatca
    11281 gctacacacc aaaatgcttc ggcagattgt tctgtagctg cgtagactcc ttcttttgaa
    11341 atatcaaatc caacagcatt ttgaatcttt ttcaattttg aaagttcatt caagaagcta
    11401 ccttctccgc agccaacatc taatagcgag acttcttcag gtaaatgctg cgcaatagtc
    11461 tcaataacgg gctgatacat gccagattgg atcattctac gccttggaac gaacattcct
    11521 tgatcgtatt ctgttttaac cgcatgggat aaaaaataaa gtgttccttt tttggaaagg
    11581 tcaaaacggt gattatt
//
LOCUS       DAZUKT010000088        11313 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000088 DAZUKT010000000
VERSION     DAZUKT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.090"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG816_002418"
     CDS             complement(<1..1031)
                     /locus_tag="ACG816_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4921262.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACG816_002419"
     CDS             complement(1113..1802)
                     /locus_tag="ACG816_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4921263.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG816_002420"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG816_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4921264.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG816_002421"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG816_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4921265.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG816_002422"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG816_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4921266.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG816_002423"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG816_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4921267.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG816_002424"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG816_002424"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4921268.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG816_002425"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG816_002425"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4921269.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACG816_002426"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACG816_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACG816_002427"
     CDS             complement(7636..8523)
                     /locus_tag="ACG816_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4921270.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG816_002428"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG816_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4921271.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG816_002429"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG816_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4921272.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG816_002430"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG816_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4921273.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG816_002431"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG816_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4921274.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACG816_002432"
     CDS             complement(10454..10957)
                     /locus_tag="ACG816_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4921275.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3305 a         2351 c         2069 g         3588 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaa
//
LOCUS       DAZUKT010000089        11269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000089 DAZUKT010000000
VERSION     DAZUKT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.091"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..531
                     /locus_tag="ACG816_002433"
     CDS             <1..531
                     /locus_tag="ACG816_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921276.1"
                     /translation="SFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILT
                     LAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSN
                     RRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFS
                     FAREDEDNPKTYDILA"
     gene            complement(592..957)
                     /locus_tag="ACG816_002434"
     CDS             complement(592..957)
                     /locus_tag="ACG816_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4921277.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1033..1278)
                     /locus_tag="ACG816_002435"
     CDS             complement(1033..1278)
                     /locus_tag="ACG816_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4921278.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1348..1602)
                     /locus_tag="ACG816_002436"
     CDS             complement(1348..1602)
                     /locus_tag="ACG816_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921279.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1766..2089)
                     /locus_tag="ACG816_002437"
     CDS             complement(1766..2089)
                     /locus_tag="ACG816_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921280.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2276..2349
                     /locus_tag="ACG816_002438"
     tRNA            2276..2349
                     /locus_tag="ACG816_002438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2310..2312,aa:Ile,seq:cat)
     gene            complement(2734..3231)
                     /locus_tag="ACG816_002439"
     CDS             complement(2734..3231)
                     /locus_tag="ACG816_002439"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4921281.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3271..4080)
                     /locus_tag="ACG816_002440"
     CDS             complement(3271..4080)
                     /locus_tag="ACG816_002440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4921282.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4414..5271
                     /locus_tag="ACG816_002441"
     CDS             4414..5271
                     /locus_tag="ACG816_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4921283.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5264..6166
                     /locus_tag="ACG816_002442"
     CDS             5264..6166
                     /locus_tag="ACG816_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4921284.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6199..6549
                     /locus_tag="ACG816_002443"
     CDS             6199..6549
                     /locus_tag="ACG816_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4921285.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6598..7521
                     /locus_tag="ACG816_002444"
     CDS             6598..7521
                     /locus_tag="ACG816_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4921286.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7599..8492
                     /locus_tag="ACG816_002445"
     CDS             7599..8492
                     /locus_tag="ACG816_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4921287.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8530..9093
                     /gene="amaP"
                     /locus_tag="ACG816_002446"
     CDS             8530..9093
                     /gene="amaP"
                     /locus_tag="ACG816_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4921288.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9107..9295
                     /locus_tag="ACG816_002447"
     CDS             9107..9295
                     /locus_tag="ACG816_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4921289.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9308..10210
                     /locus_tag="ACG816_002448"
     CDS             9308..10210
                     /locus_tag="ACG816_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4921290.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10242..10484
                     /locus_tag="ACG816_002449"
     CDS             10242..10484
                     /locus_tag="ACG816_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4921291.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10740..11111)
                     /locus_tag="ACG816_002450"
     CDS             complement(10740..11111)
                     /locus_tag="ACG816_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921292.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3994 a         1929 c         2134 g         3212 t
ORIGIN      
        1 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
       61 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      121 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      181 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      241 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      301 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      361 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      421 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
      481 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
      541 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
      601 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
      661 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
      721 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
      781 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
      841 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
      901 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga
      961 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1021 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1081 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1141 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1201 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1261 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1321 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1381 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1441 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     1501 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     1561 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     1621 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     1681 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     1741 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     1801 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     1861 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     1921 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     1981 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2041 ctaaatcgaa taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat
     2101 aaaagaataa cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg
     2161 acacaagtaa aagaaataag aataacaata ttattttact tatattttga aacttgtgag
     2221 agtttgtcat tcgtgctata gttgaaaagt aacaaaaata aattttattt atgatgtacc
     2281 tttagctcag ttggttagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta
     2341 caaggtacat aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     2401 aaaaacagct tcttatgttt ttacttggct gttcttatta ctgcagatgc aagcttttga
     2461 ataccgttta aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa
     2521 cggaaaaata gttcctcata ttttgccgtt tgttaagcta ataccgaggg tcagctgatt
     2581 gaacaccatt taagaaagag gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc
     2641 aatctacttt gatagaaaaa gcacgtagga ctaaaacagt tctgcgtgct ttttttattg
     2701 tgtgttaaat atcgtaaatg aagatagata tttttatgat tcgacgatag agacgttgaa
     2761 atctttcaac agctctaaag cggattctcc gtgttttcta ctggagacta acattggatc
     2821 aacaaaaaag tctgcttcag gaaaagcact ctgcaggcct atcagattag agagtacaca
     2881 caagtttgtt tcaatcccga ctacttcgat ctctttttgt tcttctttgc tttcaaataa
     2941 agtttcctgg atttcagcta aagtttcagg tggaatcgta aagtaatact ttttaatcac
     3001 tcgttcattt tcttgtggag aaagaagagg gatcaattgc aaatcttctc gttcttcttc
     3061 tttgtccttc agttcaactg gtatatcccg tgtgaagaga atatattcat tattttggcg
     3121 tgctttagct aatctttgag caatacgttc tgccaattga tctgactctg gaatataaaa
     3181 ttcgctgttt ggatcaagaa tatgattttg catatcaata ataactaaca ttttctttcc
     3241 tcctacattc atatcgtcat tttattcttt ttaatagaag ttttttaaaa tataagcaac
     3301 accagcttct tcattcgtca gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc
     3361 attccccatc gcaatactca acccgctgta attcaacata gcaatgtcgt tttcttgatc
     3421 gccaaaagcc atgatgtcac tcgattcgat tccaaggtaa tccgccagaa aaacaagtga
     3481 atgagcttta gtgatacctg taggcgtgat ttctaagaaa tgcggatcag agaatactga
     3541 ttggatacca tctgcttgca gatctctttg tagcttctgt agtttttctt cactcttatc
     3601 tgttaatgct aatttaagat agctaagaga aggatcttcc aacatattta aaatttgatc
     3661 aaaagaatgg ttctgtaaat tgaaatgttc ataataaggg tcatctttga ctagatttga
     3721 ttcatcgaat gtcagctgat catcttgggt ttctcggcta gcaatgatgg atattttcgc
     3781 ttcttgagca tagaagaagg cttttctcag tgtatcgcta tctatttttg aacgtctgag
     3841 aactttcgcc atttttccat ccttcatttc agcaatcagt gaaccattgg aagaaatcaa
     3901 atagccaccg attcctaatt cggaaaaaat atctttagca gaaatgctgt tccttccggt
     3961 agcgacggtt acgattccgc cattttcatc aaatttccga agtgcttgga tattttcttc
     4021 tggtatcttt ccatttcgat caagcaaggt accgtctaaa tcaatcgctg ctagtttcat
     4081 tttatcttcc tactttcttt ttacatatct attttcctgt taccttaaga ttaaatgaga
     4141 atttaaaaaa atgaaactaa cttgctttgt cattatgaat tttttcaaaa aacattctta
     4201 agaaaaaggt ggctatatat agtagttttt ggtcaatacc tttgaataaa aagccatgaa
     4261 aaacgaatag ttagcgctta ccgatgcgtt ttgtgataaa aaaattttaa aatattcaat
     4321 gaaaacaaag catatatttt gctttaaaac caaatgagga atattctaga tgtagaacag
     4381 gttagaaaaa gattagaaaa aggagtgatt ttgatggaga agagatacgg cgtagttcct
     4441 gtggtaactg cttcagacga aaattatgct ccttacttga gcgtgatgat cgcaactgca
     4501 ttagaaaatt gcaataaaac aagacgcata aaattttatg tgattgacga tggtctatca
     4561 gaatatagta aagaaggatt ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc
     4621 caattcctga cagtggaaaa agatatctac gaagacttcc tagtcagtga ccatatcaca
     4681 actaccgctt acttaagaat ttctttacca aacttattag ctaaagagga ttataagaaa
     4741 gtcctttact tagattcaga tgtgttagta ctagacgata tcgtaaaact atatgatgag
     4801 ccgttaaatg gaaaaacaat cggcgctatt atcgatccag gccaagtgaa agctttggaa
     4861 agattaggaa tcgattccga tgacttgtac ttcaattcag gagtgatggt gattgacatt
     4921 gatcaatgga ataaaaaaga aattacagaa aaaactatcc attacctcag tgaaaatggc
     4981 gatcgaatca tctatcacga ccaagatgct ttgaatgctg tactttatga agattgggaa
     5041 caacttcatc caaaatggaa catgcagact tcgctgattt ttgaacgtca tccagcacca
     5101 aatgaaaaat atgaacgtca gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt
     5161 acaggacatg ataaaccttg gaatacgttg aaagatcatc cgtataccaa tttatactta
     5221 aaaaaattag cacacagtac tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat
     5281 agcagtagtc gcaagttgta acacaaaatt cgtaccacat ttagctgcat tatttgtctc
     5341 agtattagat aattgcaacc cgtctaaatt tgttcggttc tatgtgattg acgatgatat
     5401 cgattttgaa agtaaacagc ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga
     5461 tgtggaattc ttgaaaatca ataaagaatt ctttacaaat gtggtaatca gcgatcgtat
     5521 tccagaaaca gcttattatc gtattgcgat tccggaattg ttccgcggca cagaagttga
     5581 acgtatctta tatatggatt gcgacatgat cgctttacaa gatatctcta aattatggcg
     5641 tcttgacttc ggtgattcaa tcgtagcagc tgttgaagac gctgggttcc atcaacgact
     5701 ggaaaaaatg aaaatttcag ctaaatcaat gcgttatttc aactcaggtt tgatgctgat
     5761 caatgtgaaa aaatggttag atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca
     5821 taatccagaa aaattacgat tccatgacca agatgcatta aatgctatct tacatgaccg
     5881 ttggttacca cttcatccgc gttggaatgc acaaggctat atcatggcaa aagcaaaaaa
     5941 acatccaaca gcagcaggtg aacgtgaata cgaagaaact cgcaacaatc cttacattat
     6001 ccatttttca ggacatgtga aaccgtggag caaggatttt gaagggccaa cgaagaaata
     6061 ttacgaaaaa tatgcaggga tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc
     6121 aaaatatgca aaagttcaac caaaaacaga tgtagttgtt aaataataga agaccacaac
     6181 tgaagagggg gagtaggaat ggaaattaca atgtctaaaa atgcagtaga gacgttaatt
     6241 gaaaaggttg gggaaaatac aaaaatcgca ctcgcgctta tcaatgattc cgatccattt
     6301 ctaagggaca aaggtgcttt tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg
     6361 gaatttggag aatatgcgac taaaatcgag catccattat tggacatcta tacatctaaa
     6421 ttagagcaaa attatttcgg taaacgattg aacatggact tcaacaaaca gcttgacagc
     6481 ttttcattag aaaatgaaat cgcagtactc gattataata tcaaattaaa gaactgcttc
     6541 ttcagctaat agtttcgaga aagtccaaaa aggaggtata cattgagata ctactttgtg
     6601 gatgaagaaa agaatgtaat agaaacagat gaaaaaaaat ataattggct cgtacttgat
     6661 agtgcaaagc ccgaagagat tgagcaagca atcgaagaat tcgagcttcc tgatgatatt
     6721 ttcgtaggaa ccgaatatcc ggaagaagtt tctcggatgg agcgccttta tgagacgaaa
     6781 ctagagcatc cattttcttt ggtagtgatt aatctagatc agtcagataa tcgtatcgaa
     6841 aaaaaattaa cgccaatatc ttttgtactt tccgataatc tcttgattac ttgtatcgat
     6901 agaaaaaccg aatttgtgga tcggctgctg acaaagcatg gaaatcattt aaactcgttt
     6961 gaaaagatca tcacgtatac attattagat atttacaccc attacgtgaa agaactaaga
     7021 gagatgaaaa agcgaattga cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa
     7081 gaactgtata aacaggcaga tcttgaaaga gacatcgtat atatcgatca taccttaaga
     7141 gatcaacggg aaacaatgga tcaattatgg aattctgaaa ctttcatcaa acgtttagac
     7201 gatgaacagc tactctatga tgtccatttg agacagcgac aaacagaaaa aatgatcgag
     7261 atctatcgag atttattaga gagtattggt gatctattca acgggatgat ggacaataat
     7321 ctaaatcata tcatgaaata tctagattca gctgcattaa tcatttccgt tccggcgttg
     7381 ttcgcaggga tttggggaat gaatacagga ggattaccgg gtaaaagttc tgctctaggc
     7441 tttatactcg tagtctttgg ctctttgata gcagcagtag ttttaggtta ccatttatac
     7501 cggaaagatt atacaaatta aagaatctat ttaaaacagg tatcaaacaa agaaatttgt
     7561 tttggcaata caatttaaaa actaggagga atacagcaat gacagaacca aaattaatcg
     7621 acccgcgaaa cttgtaccat acggagaaat ttcctaaaca agatcaggat acaccagctt
     7681 tacagcagaa tatgattcct gttcctgatt gtggagaaga atcctatgaa ggcaataatc
     7741 aactagaaaa ccgccgtgta ctaatcactg gtggagattc agggatcggt cgtgcagcag
     7801 cgattgcatt tgctagagaa ggggcagata tcgctcttca attcttccca ggagaagaaa
     7861 aagatgcaga ggaagtagca aaatacgtga aagatgcagg acgtaagatt gtcttactac
     7921 cttttgatct tcgtgacgaa caagcaccta aagaaatcat agataaagcc gtgagtgaac
     7981 ttggcgggct agatacgatg gtactaaacg caggacagca aatttcttgt cagtctattg
     8041 ctgagttacc acttgagcaa gtcaaagaca catttatgat aaacatcatc gctatgtttg
     8101 ctttagtgaa agaagctgtt ccgcatatcc cagcaggagg agcaatcgta acaacaactt
     8161 ctgttcaagc attcaatcca agcgaacatt tattagatta cgcagcaaca aaagcttcaa
     8221 tcgctaactt tacgatcggt ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg
     8281 gtgtagcacc tggtccaatc tggacaccat tacaactaga cgaaggacaa ccttctgatt
     8341 cgatccctga atttggtcag caaagtttac tagaacgtgc aggtcagcca ggagaattag
     8401 caccagttta tgttttctta gcatcgaata aagcaagtta tgttacagcc caaatttacg
     8461 gagtaactgg tggagaagcg atcaacttat aagaagttga tcgtttcacc cctatgagga
     8521 ggtgcgaaaa tgaataaagg tgtaaaaacg atcggagtga tcggaacatt acttttgcta
     8581 tctgtgcttt tgtttgcgat actattaagt gcaacctatc cattgccttt tcagctagag
     8641 aagtttcgat tcttttccat cactaattca tacgtgcaac aatatgtatt ttggcttgcc
     8701 gttgcatttg ctattttgac aattatcacg ttacttgttt tcctgtttta tccaaaaaca
     8761 atcggaaact ttctactgaa acatggtgat ggggagttga cgttggataa aaaagcaatt
     8821 gaaggaatgg ttcgttctca cttgcatgaa gatgaattca tccattcacc aaaagtaaat
     8881 gtcaaagcaa cgaaaaacaa gatcgccgtc aatgtaaaag gggagttgaa acgaacttca
     8941 gcactcattg gtaaaacagg aacattgatg aaagaaatcg aaaatgaagt acaacacata
     9001 ttgggaacaa aagagccagt gaaagtatcg gtgaaataca caggctttga agaaccagct
     9061 aaacgaagca atgaccgcaa acgagtagaa taggggggga ccaaaaatga aagaactatt
     9121 ttcgacatac aagctcccta tcatctttgg ggtacttgga ttgatattag ccgttttatg
     9181 gatcaccgtg ggctttttca agaccttatt gatcttgatc atggcagcga tcggcgtcgc
     9241 tatcggtttt tatctgaaac aaacgaaaat cttagatcac tattttaaat cttaggagga
     9301 aacatccatg aataacaata acaaaaataa taacaataaa atgaacaacc aaaaaccagt
     9361 gtctgcacat ccagacccaa cgacaccaaa ccaatcaaat ggtgtgaacg atgaaaagaa
     9421 caaaacagca ccagtatctg cacatccaga tccagtagtc cctaacgttc aacaaggagt
     9481 agaagagaag aaacatgatg cagaaaagca tgcacctcat catccaggaa ctccagaatc
     9541 taaagttcca gaacaacatg atgcaatcga tccttctaaa cctgtggaag agcaaaaaca
     9601 tccttctgac catgcgaaag cacaagaaaa acttagttct gaccacacaa acggacaagg
     9661 aaaacttggt tctgatcata caaataacca aggaaagcat gattctgaac aggaagattc
     9721 aatgcgcggt gagctgaatt atgaagataa agttgtgcaa aaaatcatcg gaatcgcaat
     9781 tgaacaagta gacggtttat tatctgcaaa cggtggtttc ttctctaatg tggcaggaaa
     9841 acttgtgaat acagataatg taacggctgg gatcgaaaca gaagtcggta aaaaacaagt
     9901 agcagttgat atggatgtca ttgtggaata tggcaaagat atcgaaaaaa tctttgaaga
     9961 aatgcaagaa gtaatcggca aagaagtaaa aaatatgaca catttagaag tgatcgaagt
    10021 caacgctaac gtagtggaca tcaaaacaaa agaagaattt gaaaaagatc aagaaacggt
    10081 gcaagacaaa gtaactgacg cagcgaaaaa aaccggtgaa tttgcttcga atcaaacaga
    10141 taaagtaaaa tcagcagctg gaagcggcgc acaaaaagta aaagaaaata cagaaccacg
    10201 ggttcagtaa taaggaagta aaacattagg aggaacgaaa catgggattt atttggtcac
    10261 ttatcgtcgg aggaattatc ggagcaattg caggagcaat cacgaataaa ggttcttcga
    10321 tggggattat tgcaaatgtc gtagcaggtt tagttggatc agcacttggt caagcattat
    10381 taggttcttg gggaccaagt attgcaggaa tggcaatcgt gccatcattg attggtgcca
    10441 tcattttagt actaatcgta tcattctttg ctcgaaaact gtaaaaagac ttttaaaaag
    10501 aggtcgtgaa aaagctgttc ttcatcaagt aataagaaca gccaaacaaa aatagcttct
    10561 catatttttc atttggttgg gcatattatc gaagatgcaa gcttttgaac actgtttata
    10621 cggaataaga ggctgtgaca agacttttgt cacagcctct tatttatgcg ggcaaattcc
    10681 caattagatt tttaaaagtt tttccttaaa tgtgtcttta ttgatacaga gaagcttctt
    10741 taataaaaca atgcctccct aaattttctt gagataggaa aggtgaacat acaaaataat
    10801 acgaccactt tgaaaaccca gatgatccac aaatggatcg tatgatactg atgaagtaaa
    10861 aagcaatgga tcagaaactc aaagagattg aaaaagacaa ggaagaaata atatctcttt
    10921 tttactcttg tctgttttag ccaataatag ctcgtaaagt aatctagaca aacaaataga
    10981 agtaaacaaa gtttcgtaag agcagcaatc gtatcatgtt gctctgctag acgattgacg
    11041 agcaggaaaa atgttggtaa tggaatatag atcacgagta atgaaagaaa aaagtaaaca
    11101 ataaatggca tactattcct cctaattgtg tgagttcgcc ctcattttaa aataacgata
    11161 tttgtttgtg aagctttttc gtgtcgtaac aaatgttatc taatgtattt ttaaataaat
    11221 aatcattatt atcaaaataa tgttaaaaat aaaaaagaat tgtccatca
//
LOCUS       DAZUKT010000090        10814 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000090 DAZUKT010000000
VERSION     DAZUKT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.092"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..731
                     /locus_tag="ACG816_002451"
     CDS             159..731
                     /locus_tag="ACG816_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4921293.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            876..1580
                     /locus_tag="ACG816_002452"
     CDS             876..1580
                     /locus_tag="ACG816_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4921294.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1808..2146
                     /locus_tag="ACG816_002453"
     CDS             1808..2146
                     /locus_tag="ACG816_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921295.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2131..2985
                     /gene="lgt"
                     /locus_tag="ACG816_002454"
     CDS             2131..2985
                     /gene="lgt"
                     /locus_tag="ACG816_002454"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4921296.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3132..3365
                     /locus_tag="ACG816_002455"
     CDS             3132..3365
                     /locus_tag="ACG816_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4921297.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3563..5467
                     /locus_tag="ACG816_002456"
     CDS             3563..5467
                     /locus_tag="ACG816_002456"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4921298.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5777..6139
                     /locus_tag="ACG816_002457"
     CDS             5777..6139
                     /locus_tag="ACG816_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS4921299.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6156..6767
                     /locus_tag="ACG816_002458"
     CDS             6156..6767
                     /locus_tag="ACG816_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFS4921300.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6869..7777)
                     /locus_tag="ACG816_002459"
     CDS             complement(6869..7777)
                     /locus_tag="ACG816_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4921301.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7819..8133)
                     /locus_tag="ACG816_002460"
     CDS             complement(7819..8133)
                     /locus_tag="ACG816_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4921302.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8432..10363
                     /locus_tag="ACG816_002461"
     CDS             8432..10363
                     /locus_tag="ACG816_002461"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4921303.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10656..>10814
                     /locus_tag="ACG816_002462"
     CDS             10656..>10814
                     /locus_tag="ACG816_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS4921304.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKK"
BASE COUNT         3500 a         1783 c         2286 g         3245 t
ORIGIN      
        1 gaatttaata gagaagcctg gcgtctatta agaaataatt ttcttgatga gcgtcaattt
       61 ttttaacgga aatctatcct atactaaatt cagtaaagaa aaaggaggaa tcaaagaaag
      121 ctattttaaa caaaccaaaa ttataggagg aataaatcat gactgaaaat aaagctactc
      181 aagaaagaac accccaagaa agattgcaag aagaacaaga acacaaggaa catgttcatc
      241 atacgaaaat taatgcagcg gccattgcgg atcatctttt aggcaacatg catacattac
      301 atgtgaaact gcaccaatat cactggtatg taaaaggagc caatttcttt acgttgcatg
      361 aaaaatttga agagttgtat aacgaaaatg aaaaatggtt cgacaaactt gcggagcgtc
      421 taattacttc tgggcataaa cctgcttcga caacggttga atttgaaaaa tattcgatgc
      481 tttcggaaga tgcagttaat aaatacgcta aagcagaaga catggttgaa aatattatcg
      541 aggacttcag aagcactcgt gacctaacta ttcgcgcgat tcgtttagct caagacgagg
      601 gtgacgatgc tttagaagat acattgattg cttttaaaaa tgacttagac aagaacattt
      661 ggatgctgca agcgttcatt ggtaaagaag cattagaaga tgatgacgct cttgatgagg
      721 atgaagatta aggtaaaagt agaagctgat ttagggattg gaattctttc cagtcctttt
      781 tccattttct tgatgtgcat caattttttt gtagtaagag acttatataa taaaagcagc
      841 gaatataaac aaaataaata aagggggaga atgaattgac taaacatacc catcatgaaa
      901 ttggcgacca cgcagcctgt atccgcttgg ttccaatatt caatcactta gaggatagcg
      961 cgatgaatta cattgctgaa aaagcccaca cgaaacagta tcaaaaagga gagtttctgt
     1021 ttcgttcaca agaaaaagag gatactttat atattatcaa tcgtgggaaa atccgtattt
     1081 atcgattggc agactctggc aaagagcagc tagtgcggat cttgaaccct ggtgatttca
     1141 ccggagaatg gaccttgttc aacccggatg cagtacacga ggaatatgcc gaagcaaccc
     1201 gagataccgt tgtctgtatg attcagcaaa aagatgtcca agattttctg gaacaatacc
     1261 cagctatttc tttaaaacta ttggcggaaa tgtctaaacg gttagaaagt tcagaacgtc
     1321 aaacgacaca agtagccgtg gagagtgtca ttacccgttt agttttgttt ttggcagaaa
     1381 atgtggaacc tgaaatgggc aactctccca ccatcaccct gccgatggca aaaaaggaca
     1441 ttgcttccta tttaggaacg acacctgaga ccatcagtcg caaatttgga gaattggagg
     1501 acgaaggatt gattcaacag ctgtctggga aaaggatcaa gattcaggat ttagatgatt
     1561 tattgcttta cagcgaataa tcggacacac ttttttctac acgttggtgt tctgtcgctc
     1621 ttcattaaca acgggacaga actcagtgct tttgcttcca tctgtccgtt tttggctttt
     1681 ttaagacaga acgaccttca ttcattggaa aaataaaagc acaaacatga taaccctcaa
     1741 gtttttttgt tttatttttc tttaagatag ggttgtcgaa caaaacgtaa aaataagtag
     1801 ggtgaatgtg cattgttttt gggtgatagt tgccatcacg ttgtcagcag cgggggcatt
     1861 ctttgtcgtc cctttcacca agaacacgat tggcggcgaa gcaaaaacgt ctaacgttgt
     1921 tgaggcagat aggttagcta tggaaaaaga agaggaagtt gtaaaaggag ccggcgtaat
     1981 cggacacgga aaagtggcgt attacgaagc gagtgaccag ggagataaaa gtgtattcac
     2041 tgtttggttt ccatggagct tagccggaat cttgttcagc atcaccatga ctagaatgac
     2101 gcgaaaaatt gaagaaatgg agcaaataga atgggattcg tattaggcgt tttgaatccc
     2161 catgtggtgg agattggttc tgttgtgatt cattggtatg gagtcattat tgcagcgggc
     2221 atattagcgg ctctccagtt gagcaccaag gaagcaaaga aaaaaggact ggaagaggat
     2281 accattatcg atatggcctt atgggcaatt ccgattggac tcctcggcgc tcggctttat
     2341 tatgtgttat ttgaacttgg ttattatctg caaaatccag gacaaatcct tgcgatttgg
     2401 aatggcggca ttgcgattta tgggggattg attgcaggag ggggtacgct atattggtat
     2461 gcaaagaaaa aaggtacttc tttggcactc gttcttgata ttttagcgcc caatgtattg
     2521 ttagcccagt ccattggtcg ttggggcaat tttatgaatc aggaagctca tggtgggccg
     2581 gttactcgtc aattcttgga aaatctttat ttacctgaat ttatcattga acaaatgaat
     2641 atgaatggta cctactacca tccgactttt ttatacgagt cattgtggag tttgttgggc
     2701 tttgtcctca tcgtcactat acggaaccga aaacagcttt tgcgtcaagg agaagtcgct
     2761 ttgagttacg ttttgtggta ctcagtgggt cggttcttca ttgaaggtat gcgaacggac
     2821 agtttatgga ttggcgagtg gatccgcgtt tcgcaagcct tgtctctggc cctgtttatt
     2881 ggcgcgattg tagtatggtt tatacgcaga caagattatc caccaatttc ttattattct
     2941 gatggcaata aggggcagaa aaagactatt aagatggtga attgagcaaa aagataaaaa
     3001 gaagtgcaaa acttgatatg cgtcaatttt cgtttgctaa aagtgagcta tactacagtt
     3061 gtaaagagga aaaggcagtg ttcattaatc tgctgccgat aaaataaaaa cacataaagg
     3121 agataggaat tatggaaaaa gctatattgc aattagaaac gttgtcttgt ccaagttgta
     3181 tgcaaaaaat tgaaggtgcc gtgaaatcag ttaacggtat tgataaagac agcattaaag
     3241 tattattcaa ctccagcaaa gtcaaaacca attttgattc agctgtcact tcgattgaag
     3301 agattcaaaa agctatcgaa aacgtaggct atccggttct taaagcaaaa gtcaaggccg
     3361 tttaacagat taaatgaact aggcaattct ggaagaaaag ctatttttcc aggattgctt
     3421 tttttgttgc aatccgattc gaggtgctta gaaacgataa aagagaactt tctaaacttg
     3481 atgtcagtca attaattact taggcaatta tcttatactg tgtttatcaa taaaagagaa
     3541 attaaatttg gagggaaaaa gcatgcaaca atatatatta agcaagaaaa atgttatcac
     3601 cgtcatcagt ggattgttaa tcgtactcgg tttcttcagt cactttgttt tagaaaacgt
     3661 aggactttca gagtggtctt tgatcatcgc ctctgtcttt gggattacgc caattgccat
     3721 tcaagcgttt caagcaatga aagtcaaagt catcagtatt gatgtcttag tcagtattgc
     3781 agcgattggt gcgcttttta ttcaaaatta tgaagaatcg gctattgtca cgttcttatt
     3841 cttattcgga cactacttgg aacaacgaac attgaaccaa acgcgatctg ctatcaaaga
     3901 attgactgaa atggcacccg aaagcgcctt gaaacaaatg gataatggcg aatttgaaga
     3961 agtagaagtg gatgatatag atgaagggga tatcttgctc gttaaaacgg gtgcgaaagt
     4021 tccagtagat ggcatagtcc ttacgggtga agggcatatc aatgaagcta gcattacagg
     4081 tgaagctatt ccggtcaaca agctagctga tgcagaagtt tttgcaggaa ccattttaga
     4141 aaacggaacg attcaaatca gagctgaccg agttggtgag gacaccacat ttggaaaaat
     4201 tattgaactc gtggaagaag cacaagattc taaatccgaa gcggaacggt tcattgatcg
     4261 cttttctaaa tactacacac cagctgtctt ggttctggca attgttgtat gggcgttcag
     4321 tcaaaatatt gagttagcaa tcaccatctt ggttctaggt tgcccaggcg cattagttat
     4381 cggagtgcct gtctcaaacg ttgccggtat tgggaatggt gctcgtaacg gtgttctttt
     4441 aaaagggagt gaagtcattc aagacttcag taatgtggat acaattgtat ttgataagac
     4501 aggtacttta actgtcggaa acccaaccgt tgcagcgact gaactgtatg aaaaagatcc
     4561 tgctgaaacg cttggctatc tggccagtgt ggaacgggaa tcagatcatc cattagcaaa
     4621 agcggtctta aatcaaattg gagaaacaaa cttttatcct gttgaaggaa ctgaagttgt
     4681 gaaaggcggc ggaatcgttt caagtgtagc tggacataga gtggctgttg ggaatgtggc
     4741 cttgatggaa aaagaaaatg tcactctcag caaaaaagtg caaaaagatg tcaaacggtt
     4801 tgaacaacaa gggaactctc tcgttttgac agcggtcgac ggtgaattaa aagtactgat
     4861 gggcattcgc gatcaagtcc gtccgggtgt gaaagctaat ttgcaggaat taaaagactt
     4921 gggcgtgaaa aatctagtcg ttctttcagg agataaccaa ggaaccgttg atgtggtcgc
     4981 cggcgaactt ggtttgaccg aagctcacgg ctacatgttg ccggaagaca aatcggctta
     5041 tatcggaaaa cttcaacaac gtggtcaaat tgtagccttt gttggagatg gcgttaacga
     5101 tagtccgtcc ttagctttag cagacattgg aatcgcaatg ggaagcggaa cggacgtagc
     5161 gattgaaaca tccgatgtcg ttttaatgaa ctcgaacttc agcaacttgc ctcacgcatt
     5221 aggattagta aaagccaccg caaataatat gaaacaaaat atcgtgattt caattggagt
     5281 agtgttggtc ttgttgacca gcgtcttctt cagcgaatgg atgaatatgt ctatcggaat
     5341 gttggttcat gaaggtagta tcttggtggt aattttcaat ggcatgagat taatgaagta
     5401 taagttgaaa ggtcgaaaag aacaaaaaga agtatcagca cctgcagaga aagtaaaaat
     5461 atcttaagaa taaattagaa gaggctgaga caatagtcca tccttgaaat gacaaaagcg
     5521 gaaactcaga tttttaaatc tgggattttc cgcttttttg agttcttgaa aaaaaataag
     5581 agtatagagt atgttcttta taaaattaat tttcgtgtaa atgattaaaa aatttaataa
     5641 taaaatttaa attcctatta agttgtaaca catgaaattg cttataatag tgtattgaaa
     5701 ttaaggtaga ttgacaaacg agtgatcaaa tagatatact accttcaaac gaccgtttga
     5761 atgtgaggtg agatgaatgg aaaaagtttg ccaagtgact atcgtacata aagaaaaagt
     5821 aaatgtaata aaaaaaaaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     5881 atgtttcagc gattcttcta ggattaaaat tttctatgct ttagaaacct ataaggaaat
     5941 gtgtgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     6001 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     6061 ttctttagcg aatgaagaca tcctttccgc tattcgtgtt tttttaaagt tatcagaaaa
     6121 tctatctatg aaatcttagt ttggaggaag ttattttgtt acaaagtatt ttttcagcta
     6181 tcgctgttta tatttctacc agtattgatt atttgtttat cttgctgatt atcttttctc
     6241 aaagccacac aaaaaaaggt attcgtcaca ttttttgggg tcaatatctt ggaacaggta
     6301 ttttggtagc cgtaagtttg tttgctgctt atgtgctaaa ttttattccg caagattgga
     6361 tcattggtct tctcggtcta attccgattt tcttaggaat cagagtcgct tttgttggcg
     6421 aagaggagga agaagaagag gaagtcgttg aaaaactcga atctagagga actaatcgct
     6481 tattctggac agttgcctta ataacgattg cttctggagg cgataattta ggtatttata
     6541 ttccatactt tacctcgtta gctttttcag aaatcattat tgctttaatc gtattcgcta
     6601 tctctgttgc ggttctctgc tatatcagtt acaaattagc caagatttct tttgtttccg
     6661 aaacgttaga aaaatacgaa cgaatcattg tccctataat gtttattgcg ttaggaattt
     6721 ttattatgat agagaacggg acgatacaaa cgctactagg tttttaaaaa tcataatcac
     6781 gaaagataaa aagagaggca gcgactaatc aaaatcgctg ccttttttgc ttttcaaata
     6841 atgaagtatt ttttagcgta gaactatctt acgtgttgtg ggagtatccc ttttcaagct
     6901 cttctttgct tgtagttatt tctattgtga tatgattctc tacgcttgat ttgtgtgcga
     6961 cttctgcaat ttcttctttt atttccctag ccttgtctat gtctgaaggg tctataagaa
     7021 ccgtgatgat aagggcgttt tcttccccgt ccagtgacca aatgtgaaaa tgtgagaggt
     7081 cttttacagc aggaacattt ttaatggagt ccgccaagct ctttacgttt acattttcag
     7141 gcgaaccatt caataaaatt ttcattgtgg tgaaaaattc aggaacggtt ttgtagagga
     7201 tataaagtgc aatcaaaatc gataagatag gatccagacg atatgcctcg gtaaagttca
     7261 ttagaatact cacaatcaag actccaatcc accctaatac gtcttccagc atatggaggt
     7321 tcaacatgct ctcgtttttg gatgtgcctt tgcttaaaat ccacgcagca tatccattca
     7381 atgcaatagc tacgagagaa agccagaaca tgccactata atttacgggt tgtgggtcaa
     7441 tcaaacgtgg cacactacga tagatcataa aaaacgagcc actaattagc acaacgccag
     7501 ttatcagcgc gcccagtaaa gaaaagcggt tatagccaaa agtgaaccgt tcatcttgtt
     7561 ttttctctga ataaccttga aaaaaccaag cgaatccaat tgaaatagag tcccctaaat
     7621 catgaactgc gtcggacata atggacacac tattgaaaag aaagccaaag aaaaattcgc
     7681 ttatagagaa aataagattt atgaagaaag caattttaat attctttttc ggttgattct
     7741 gattcgagtg attatggtct ttatctgatt ttgacattta aaaacctcct aagtttgtat
     7801 gctacatact aattggtttt atttttctaa ttcattaaca tgagttaaga cttgttcgag
     7861 aatactaaaa acatgaaggt catctaaact gtaaagcata ctttttcctt ctcttcttga
     7921 tttcactaag cgtgcgtctt tcaacgtttt taattgatga gaaatagcgg actgttccat
     7981 gtccagagaa atagcgatat ttccaacact acactctttt ttctgtaaaa gaaagagaac
     8041 cgacaatcgt gtaggatcac taatcacctt aaatagtttg cttattgaca gaatagatcc
     8101 tttatcgatt ggagaagttg tttttatatc catagtgttt ttattccttt catatgacct
     8161 tccgttcata tctatattac catatgagcg aaacaatgct aatcttttgt ttataaaaga
     8221 gaagtggcgt tctctttatc ttgacaaaat gtatatagcg atataaaatg agtatacaaa
     8281 catatgatta attagtcata tgtaaggtgg attcattacg atttactttt ggaataaacg
     8341 aaaggagtca gaaatagcag aaatttatgt gattcaagaa gtaaatgatg gaaaacaaag
     8401 gaattataaa ttatataggg aggcaataga agtggaaact atccaagaac aacagtctca
     8461 agaaaagcat aatcataatc acaaccacga tcacgaccat gatcatggga aaatgccagt
     8521 cgttttgtac tttgttggtt tagcattagc agtgattgct ctatttttga atgaagatta
     8581 tcagttggta caaaatgttt tgttctcaat cgctacaatc agtgccgggt atcacgttat
     8641 tcttcttgaa ggccttggag aaacaattga gaattcaaaa gcacaaaaaa agttcatacc
     8701 taattctcat attttaatgg gattagcggc aattggagct tctttgatgg ggaatttttg
     8761 ggaaggaaca ttattgattc ttattttttc tggcgcacat tttttggaag attatgcaga
     8821 aggaagaagc aaacgagaaa taacgaaatt actcgaaatg aatccaacga ctgcccggtt
     8881 aatcctgcct gatggtagta caaaaaatgt ggatgtcagt gaattaaaag tgggagatca
     8941 acttcaagtg ttgaatggcg atcaagtacc tattgatggc gtcattttgt ctggatctac
     9001 atccattgat gagtcgtcta ttactggaga aagtatcccg aaagagaaat ccaaaggaga
     9061 tggcgtcttc gggagtacaa tcaatggaac aggtaccttc acgatgaaag tcaccaaaga
     9121 aaacaaagat acagtatttt caaaaatttt acaattagtg aatcagaacc aagataacca
     9181 aacaaaagct gcgagtatta tccaaaaatt tgaaccgaaa tatgtcacag ttgttttaat
     9241 tgccattcct ctattcatgt tattggctcc ttttctactt gattggacat ggtcacaaag
     9301 tgtctataga ggattggtcc ttttagttgc agcttcaccg tgtgctttag cagcagctac
     9361 tgtctcggca acgctatcga cgacttctaa tttagccaaa aaaggagtcc tttcaaaagg
     9421 tagttcttac ctgtctcaat tagccgatat taaagcaatt gcatttgata agacgggcac
     9481 cttgaccaaa ggaaaacctg aagtaaccaa ttattatttt gctgattccg tgaacgaaga
     9541 aaacatgatt gatatcgtag tagctcttga aaaagaatcc aatcacccct tagcagatgc
     9601 tattctaaga aagtttgagc aaaaaaacaa actaactatt gaagtagaaa accaaattgg
     9661 taaaggattg acgggagatt acaaagggag aaattatcgt ataggaaaac cgacttcgtt
     9721 tgatgatgtt tcagatgaat atagtcgttt aaataatgaa tgggcgtcag aaggtaagac
     9781 agtcgtatac gtagcagtag atgaagaggt tataggcctt attgccctta tggacgttcc
     9841 gagtgaatat gctaaagaaa ccattgaata ttttaggaaa caaggtatac acaccacatt
     9901 aattactggt gactcagaaa tgacgggaaa agcggttgct aaacaattag gaatagatga
     9961 agtgattgcg aatgtcatgc cggaagacaa atccagaatt atagacgaac aaaaagaaaa
    10021 atacggcgtg accgccatgg ttggagacgg tgtaaatgat gctcctgctc ttgttaatgc
    10081 agatgtggga atcgctatgg gagatgggac tgatgtggca gtagaggtat ctgatttagt
    10141 tttaatgcaa aacaatttat ctaaattggt acaatctcat aatatttctt cgaaaatgaa
    10201 ccgtgttatt tggcagaata ttattttttc aatggccgtt gttgcttttt taattgtcgt
    10261 tagtttctta ggcttaacgg atattgcaat cagtgtcatt attcatgaag gaagtacttt
    10321 ggttgttatt ttgaatggac ttcgattgct aaaggcagtc taaagaatga ttgcacaact
    10381 tcaaattaat cgagtaatag agataattaa ggattacact acatttttta tctctattac
    10441 ttgtttatat ttatctagat gttcaaagtc aaaatgatat atcaaccata ctcttgtggc
    10501 gcgggttgaa tataagatac atgaaattgg ataaaatcat gtatcaagaa tcctatcctt
    10561 atagggttct ttttttttac tccccctaaa cacacgattt cgtgttgtaa taactataat
    10621 catgtattcg aacagactaa aaaggaggga ttgtcatggg ctataatgtt cagccactac
    10681 ggtcgcaaca ggagattaat gattttttat tttgcctgcg acggaataaa aatgcggaac
    10741 gtgacgtttt cttgtttcta attggaatta acagcggtct gcgcatgtcc gacatcgtta
    10801 aactcaagaa aaaa
//
LOCUS       DAZUKT010000091        10420 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000091 DAZUKT010000000
VERSION     DAZUKT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.093"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1146
                     /locus_tag="ACG816_002463"
     CDS             103..1146
                     /locus_tag="ACG816_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4921305.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1319..1543
                     /locus_tag="ACG816_002464"
     CDS             1319..1543
                     /locus_tag="ACG816_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921306.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1546..1803
                     /locus_tag="ACG816_002465"
     CDS             1546..1803
                     /locus_tag="ACG816_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921307.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2020..2895
                     /locus_tag="ACG816_002466"
     CDS             2020..2895
                     /locus_tag="ACG816_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921308.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2905..3699
                     /locus_tag="ACG816_002467"
     CDS             2905..3699
                     /locus_tag="ACG816_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921309.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3998..4846
                     /locus_tag="ACG816_002468"
     CDS             3998..4846
                     /locus_tag="ACG816_002468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921310.1"
                     /translation="MIEYDFNEAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKV
                     RPYENLRTYMFFLVNFEGTAKEGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLI
                     LDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQK
                     SLREGETSTFDKYSMYNRAYSSMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRS
                     EVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKGFENKGSFGKSKDEVKGVVIDDD
                     FEFDFD"
     gene            4879..5199
                     /locus_tag="ACG816_002469"
     CDS             4879..5199
                     /locus_tag="ACG816_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921311.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5454..6143
                     /locus_tag="ACG816_002470"
     CDS             5454..6143
                     /locus_tag="ACG816_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFS4921312.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6144..6347
                     /locus_tag="ACG816_002471"
     CDS             6144..6347
                     /locus_tag="ACG816_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921313.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            6367..7995
                     /locus_tag="ACG816_002472"
     CDS             6367..7995
                     /locus_tag="ACG816_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921314.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVIFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8046..8417
                     /locus_tag="ACG816_002473"
     CDS             8046..8417
                     /locus_tag="ACG816_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921315.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8502..8702
                     /locus_tag="ACG816_002474"
     CDS             8502..8702
                     /locus_tag="ACG816_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921316.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8836..10062
                     /locus_tag="ACG816_002475"
     CDS             8836..10062
                     /locus_tag="ACG816_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921317.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
BASE COUNT         4070 a         1263 c         2093 g         2994 t
ORIGIN      
        1 aacaacgatt atgatcttaa aacaatcgtt tatgtaattg gcaatcttac gaataatttt
       61 catgcaataa tgacaataaa aaatgaaaag gggtaaaatc aaatgaaaaa atttttagta
      121 ggaattctat tcacaatggg gctagtatta ttatcacctt cagtttcagc agaaagctct
      181 atttccatgt atcgtatgta taatcccaat agcggagagc atctatacac tagaagcgtt
      241 ggagaaagag acaatctgaa acgttatgga tggcattacg aaggtgtcgc atggcaagct
      301 ccaacaagtg gtactcctgt ttatcgtttg tataatcggt ataacggtga acatttttac
      361 acagttgatg ccggagaaag agataagatt tcacacacag gatggacgta cgaaggtgtt
      421 gcgttctatt cctacaacgg agcaaataga gttcctgtaa cacgtttata taacaaatac
      481 gtgaattggc atcactacac gttagatcaa aatgaaaaga acgtaattag taaacaaggt
      541 tggaagattg aaggtacagg ctggtatgca atgccaaaca gcgtaccaaa tcctacacct
      601 gctccaagca ctgaaaaagt gttgaacgtt cctgttgtat ggcaaggaaa tacaatgtta
      661 tgcgaaggag cttctttatt agaaggttta cactacaaag gtgtagccac aaatcaaagc
      721 ttaaccagtt ttgtaaacta tatgccaaaa gctaatgaca ataatcctta tcatggttac
      781 tcaggagagt ggcgtcataa cgttaatgga acgtatcaag gcatgatggc tgacccagta
      841 gttaaatggg caaaaaacgt tggcggtaat gccgtcaata tcactggttc aggaacgaat
      901 ggaatcaaga atgaaattcg caatggtaat ccggttgtaa cttggattac ttataactat
      961 gctacgccag agttcaaaca aatgccttgg ggtcgtgcag tatggaatgg acatgtagtt
     1021 ctcgtggatg gcttcagaaa tggtgcatac catgttgttg accctgtatt tggcgcaaaa
     1081 tggatcaatg caagcacgta tgagagagca tttaatgtga ctggaatggc ggttgctgta
     1141 cgttaattga tagaattatc aactaaatga aataagagct taattactga aatggtaaat
     1201 aggctcttat ttttttagag gaaatttttg taatacgaat ctataacaaa aaatattgaa
     1261 tgtagtacac aaaagtggta tattaaaatc gtagagatta tgcgaagagg tgattttgat
     1321 gaaccaaaga agaagaaaaa ggttagaaga aataagcgag cgactttccg agctaaagga
     1381 tgagttagaa gtaattcaag aagaagagta caaagaaaat atacctaaaa atctacaggg
     1441 atcagaaaga tatgaaaaag cagaagaatc cgttgatcaa cttgaagaag ctgttgatgg
     1501 tttatccgac atattagaat tgattgaaga tatagctagc tagagatgat taatgaagat
     1561 ttcaggagtg agaataaaat gattgatatt tcactgatag agaagctact tttttcaaat
     1621 aagtacacag ccaaagagtt ggctacaaga tggcaaatat ccgaatcagc tattaaagcc
     1681 tatcgtcaaa ataagggaag cgtaggatgg cgtgattgga aaggaatcag cattggaaaa
     1741 gcagaagaca taatgaaacg tgaaggattt caagctgaca atgattctaa aaaagttgaa
     1801 tagtttaata gtcgccaaga aaagtaccag gacgagggga aaggtagatt acaaaggaaa
     1861 acacttttaa ataaacttcg caaaagcatc aggttatgag tggctagggc tatacgaatg
     1921 tgatgaaaac aaagggcttt aatgtcaaag aaaacgcaat agggggggaa caaaattttt
     1981 aaaaaagata tttatgtttg gtttaatgga aaaaaatata tgactactaa caaaaggtgg
     2041 tcagagctag tacaaaatac agttaaaaaa gtcatgccag atggatatca tttcaactat
     2101 atacaatgct cacaaacaac tgaaagtatt tatttttcaa tattaaatga gaatagactc
     2161 tattatttca gggtaagttc acaccatagg aaaaagagag aagataatgt tacgacattt
     2221 ttatcgggaa aatacaatgg cttcaaagag ttaagtgaag ccattggtga gtatctaaaa
     2281 agtaatagat ttgtcagaat ttctgacaaa catatattga tcttgttact ttatttgaat
     2341 aatagcttca aattgttttg gagagacgac actttctact atattgagaa cggcatggat
     2401 atttattctg gtatcaacgg catattaaaa cattatatta aggcgttact tcgatatggg
     2461 gttctttatc aagaagattt aagtaaccaa attaagatta gtaattctgg aaagtcatta
     2521 ctggcaaaat ataaaaaatt gctttctcct atctacaaag gaatagatat ggaagctgta
     2581 aattctgaaa ctttatttaa tttagttgtt gatgaaaagt ttgcgaatga attttttaaa
     2641 agtaaaaaaa ttgaaagact aaataacctg tttcctgaaa atgcaataaa agcttatgta
     2701 gataataata aatcacgatt atacaactgg tttattccca aacggttgcg aggaaaaatc
     2761 aaaattggcg atcaagtgaa agtaattaat ctaaatcata ctaagatcgt taccgtaacg
     2821 gagttgtata tagctgaaaa agaagatatt acaaaaatga aaccagttat taaaatacta
     2881 gataacaaag agtgagtgga aaatatggta gcaaaaaaga atactctaaa agaagcttct
     2941 gaaaaatatc aagatatgaa aagacgagga ttaataccaa aggacaaaaa aggtatatgg
     3001 agagaatttg ttaaggaagt cagtagtgat cgaagtggcg gtgactttgt tggacgatat
     3061 acagcaaaag gttatagcag gcgcttaagg atcactgttg aatatggaac gaatgaatca
     3121 gctacagagc tgaagcaaag gtacttagat acacgaagag aagcttcaaa aaaatatgat
     3181 gatttcaaga gcagaggtct aattcctaag aagaaaaagg ggatttggcg tgagtttttc
     3241 aaagaagaag atagcaatcg gaataaggga gacttcattg gacgatacac agcaaaaggc
     3301 tacactaaga atttagggat caaggttgaa tacggaactg gtgaaagttc atgggaattg
     3361 agaaatcgct atttaaatac attgttagag aaatataaag cagaattatt ggagttgttc
     3421 gatgttaatg attttatcga gatggctgaa aagtatggtc atgaagaagg ttttatgatc
     3481 aatgacatta tggttattga taattttacg gatttattag cacttgcgaa aaaagcaagg
     3541 gatttaagag aaacgcagat gtatctattc agaaataaaa acggaagaaa ccggagaact
     3601 gaatcgagtg agattgaaga ttactatgat gcctttacac acgatctggg agcctttgta
     3661 gacgagataa gtggtgtaat gaacaaatat agggtctaga ataaaaaagc cggagatgag
     3721 cagataaatg ggaaacaata gcatatacac acctgaaatg cagaataaaa ttaaaagact
     3781 tgtcggatta gggtggatga caagaggaaa attagattct tttagtacga tcaaatatct
     3841 tttgatggac atttaccgtc agaacaagca aacaggaata cattttttgg gctatgataa
     3901 gccggaagat gcacctttct ttgttccaag ttcagcctat agagccagta cctccggtag
     3961 catgtataac ttggatttgt ttgaggaagg agcaaagatg atcgaatatg atttcaacga
     4021 agcctattcg aggatcatga gaaattatcc tttgccgtca aacacttatt tgaaagcaaa
     4081 atataataca gatcaagcta ttttgagagc gataaataga ccaaaagtaa gaccgtatga
     4141 aaatttaaga acgtatatgt tctttttggt taattttgaa ggaacagcca aagaaggaac
     4201 atatgctaat ccaaaatcac agtcgatgtt tgcggattat ggcgaaaatc ccaaaacagc
     4261 taatgagcct atttggttat cggagattga gctaacactt atccttgatt attatgatat
     4321 taaagagatg gaagtcctag atagccacac atttttgacg aaaaaaggat tactcaaaga
     4381 atattttgaa agaatcgagc cattaaaaac tgatcctgat acgttgaagc tttataaaaa
     4441 tcttagaacg aaagtatttg gtgctatcgg acaaaaatca ttaagagaag gagaaacaag
     4501 caccttcgat aaatacagta tgtataatcg agcgtattcc tccatggtag caggcgtatt
     4561 tagagatcgt atggttagat atgaacagaa atacgttcat agcgcatatg ggctattaaa
     4621 aattcgtact gatggattgt atttcagatc cgaagtacca gagtttgaag agctgtataa
     4681 acttggagta gttaagaaaa aagttcatac aatatccaaa gaagaagtcg aaaaagcttt
     4741 agaatttaaa aacaaaggat ttgaaaataa gggctccttt gggaaatcta aagatgaagt
     4801 taagggtgtt gtgatagacg atgatttcga gtttgatttt gactaaatac tctttggaaa
     4861 agatggtagg aggtaaatat gaagaagaaa attgtggaag gaaatgtagg tgttattatt
     4921 ctaaaagatt caactgacta tgctaaatca atggcactaa aagacgaaaa agttgcacgc
     4981 atggataaag atttagttga atttctgatg gatgatacag aaagattaaa aggggatcag
     5041 tttttgagtt taatgaaaga caaaggctta aacacatatg gtttatttga ttataagaaa
     5101 ttagctatag agtgggtaaa agaaggagaa atatttttta ttgaagaatg ggaaggtact
     5161 gagaacttag ttcttcatca gtatcttttg aaagcttaat ctaagtaaaa cagttctgac
     5221 tagttatgct tgtcagaatt tcaaaggaag ttttccataa ccatcatcat tcaaaaataa
     5281 aggatttgaa aataagggtt cctttgggaa atctaaagat gaagttaagg gtgttgtgat
     5341 agacgatgat ttcaagtttg attttgacta gatttttttg caatgtttaa gagtaaacaa
     5401 ggtaagaact aagatcatga agattgagag taaagaagat agaggacgaa tgtatgagaa
     5461 cacactatat ttatttagaa gataaaattg tagatgaaat tccggcaaaa agtcgagaac
     5521 aagctagaaa attatttatt gaaaaataca aagagatcaa tggagatagt gaagttccaa
     5581 aagtggtcgt aacagttaga aaaaggcaaa caaaaaagag aagtacgttt catgtcagcc
     5641 ctgatatata taatcttata gagcgacata gaagtgaaga taatatttct atctctcaat
     5701 atattgctac agttattgat gaattgattt caggaaagta tgactatgct tttgattttg
     5761 agggtgaaaa ggattacaaa agtgttgctg tctttcaatc tgattttgag aagctaaaaa
     5821 actatgctgg caggagaaat gttaattttg caagactact agaagagttc tactccgaag
     5881 agttgggagt aggaaatgaa tacaagagag aaacttatgc gaaattgtct cataagttat
     5941 cccaagcgct taaagaagtg acagaagtta aagcggaact agatgctaaa cttgaagaag
     6001 tgaaaaaata tcaaacagag aggaagaaca acatgaagaa tcaagtggaa tttgacgaac
     6061 agaaagaatt tgaaaaagca agaaaacgct ataacaaaat tatgaatgaa cttagctatg
     6121 aacacttaac agttcatgaa taattgagtg agggtacaga aaaatatacg atcgcagata
     6181 tggttaatga agtggaaact cttttagctt ctttctttga aaatggtcac gtaaacgatg
     6241 aatggcgaaa agaagaacct aaaagggtta acgcttatat tgctcgatta aaaaggttta
     6301 ttgataaata taaggagaat agtgacaaat tttctaaaga taaataagga gagagtgtga
     6361 ttacatttgc cagaaatcga aggaatattt tcgccaaaaa taggtaaaaa acagttagat
     6421 ttcagcagtt ttccgagcgt aaatagagat tataatctag gtgatgaggt acagatacta
     6481 aatattgatt tcaaagaact agacttcgct gttgctgttt ccaacaaacc caaggcattg
     6541 gaacgagtga aaaaaatata ccaagagaat gatcaaaata tcaaaaatat gatcaaaaag
     6601 tatagacgag ataattcaaa agttgaagac gttttattca acggtacggc aaaacatgat
     6661 ttgaatgttt atatggcatt aggagtggct ttgtactgta attcaattaa tcagacagat
     6721 attttggtta atatcgtgag gaatagctat caaaaaatat taaaaataag tagcgaaaca
     6781 aacccttatt atgcaatgaa atatcttggg gataatttat taaatgataa aaaattgatg
     6841 cacaacactg aagagctgtt atctaatttg gctgcgatta actttctatt caaaacaggt
     6901 tctgaagatt attttataga taacgtacta aaaggtgctt acggaacagc attttatccg
     6961 ctttacaata tgaaacagca taaagccgaa caaaaggata acaaacttga aattaaaaag
     7021 atgaaagaac tattaggtat aggatttgta aaacccaatt caaaagtcac tattgcttcg
     7081 ttagcaacta ctaacaatga tgggaaaata gtaaatgggt ttgacgattc agatatagat
     7141 atattgtcaa aatcatattt ttctttgtat ggagattttg cagatttgat gggtattgat
     7201 agttacttag tcaataattt tgagatcaag ggtagcgagc tgaatgatat tgtgtatttc
     7261 gttttaatct ctctatatgc aattgatcag aataccgagt accgacaaat tgactatttg
     7321 ctagtttttt atagtttgtt atatcagttc gttttaactc ggttgtttaa aaatgctaag
     7381 gaagaattga tcctgaattc tgattatgaa atagcaaaac aaattgcaag tattagaaaa
     7441 gaaaacaaat ctgaagtaat caaattaaga aaagaattaa gtattgcgag taaagaaaac
     7501 ggtcggttag aatctttagt caaatcggca aaaaaagaag aggaactttt gaagcaagag
     7561 attaaagaat taaaaggaat cagaaaagaa cttgaacagc tgaggtttga aaataaagta
     7621 ttaaagcaat tgcaggagca agaacataga caatcattaa atggtaaaac tgaagagcct
     7681 tcactgtctg aaatgatagc ttatctaaaa cataaaaaca ttcttttagt gggaggacat
     7741 gtaatttttc aaaataaatt aactgaacta ttaccaaata ttaaaatgtt tgatgtaagc
     7801 gaaaataatg gaaagatact taaaaatttg aatacgtgtg atgcgatttt tctatataca
     7861 gattacttga atcatggtct atattatcgt gttatgagtg aaatttctaa taaagataag
     7921 atattttatt tggataatca tgtgaatatg aacttaacaa ttaaacgtat ttacgatctt
     7981 ttatcactta tataaagaga gaagtgataa ataggacaca aaatttggaa agtaggtaag
     8041 tcactatgag agaactaact ttatcttcta aggaattaga aaagaaaact gctttattaa
     8101 acgaaatatt tagtgctgat tgtataatcc aaagtttaaa agaaagttca ttttcaaaac
     8161 taacagatca ggaaaaaatt tcttatatgc taagtttaga taatctagga actaaatatg
     8221 atgaatcagc aagtagcgat aagcttttga ttgaattagt atcaagtctt agtagttttg
     8281 gaaaaatgaa aagatttaat gtgatattag aaatttttgc ttggttaatc ccttcattta
     8341 tttggttttt ttatattttc atagttgaat ctgagttaat agccaaggtc tctcttggac
     8401 tgttaattcc gatataaata ggttcagcaa tttactttaa taacttgtat acacgtattc
     8461 aaataaaaaa gcagttatta catgaaggat taagtggtaa tatgttaaaa agaattgcat
     8521 gggataaaga taataaaaca gatattgagc ttaaagaata taaatacaat tatttctcga
     8581 gatgtataga tagaaagtta ctgtttaaaa aagaaggatt agaatttagt tttgatgaag
     8641 atgaatttga aaaattagtg atggactggc gtaagaattt actggatacg gtacaattct
     8701 aaaagcaatt gaggtcgaaa ttccgatact tgaatcatga tctatattat cgtgttatga
     8761 gtgaaatttc taataaagat aagatatttc atttggacag tcatgtgaat atgagcttaa
     8821 caattaaacg tatttatgat cttttatatg tttataaaca ttaatgaggt gctaaaattg
     8881 acaacaataa ctatgacaaa aaaaatagaa gtatatgttg atggatcaca agaagaaagg
     8941 gattcctttt ttgatttttt ttggaaaaat agtagagaat atgtaaaagc gttgaatctg
     9001 gtatataaac agctaatgtt tgaagagttg ataaaagatg caattaaaga aatggatcaa
     9061 ggttatgctg agagagaaga aagtattcaa aatcggattg aaaagttagc atatactgat
     9121 ccaatagatc aaaaaaaact tgataaagca aaagctgaat tatctaaatt gagaaatgaa
     9181 aaaaataaag aagcgagaga aatattaacg caagcgatag ggttgaagag tcaaacgaga
     9241 acacgagatg tagcaaaaaa attagacttt gactttggag attttgttga ttccgcaaat
     9301 atgaaagcta gtaaagattt tggcaatgat tttatcggaa ttgtgaccgg acaaagaacg
     9361 cctagaatat ataaaaatag aaacaaatat ggattactac cttttagagg tcgagatatt
     9421 aaaatccata aagatgataa gttttatatt tatttaccta aaggctttag gtttaaagtg
     9481 aatttaggta gcaagccaag aaaagcaaga gatgttgctt cagttttaca acacattatg
     9541 gatggcgatt acaaattaaa tcaaagtaat cttatttttg atggaagaaa attatttttt
     9601 aatctaacaa tttcttttga aaaaagctat aatgaaatgg aattcaagaa aggtaatgaa
     9661 ttgatcgtaa actttgcaga tttgtgtagc ggtgaatgtt taattaatga taaatctgta
     9721 atgtcttttg gtgatagcca atatataaga aacttcaaaa gtaaaatttt agcaatgaaa
     9781 tcaaaagaac aaagccgagg agtcttttct aagggcggtc atggtcgaaa aagaaaatta
     9841 gatcaagatc gatgggaagc aataaagaaa agagaaagta attttgttaa aacttataat
     9901 aatcaatgtt ctgcaaaaat cgttaaaact gctattcgca ataaagctca aattattcgt
     9961 atcattgaac cagataaggc ttatggcgaa tgggcttact atcaattgaa agaacaacta
    10021 gtaaacaaag gaaaaagata tgggatagaa gtcagaagtt agtaaattaa acaatcgttg
    10081 atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc cgtcaagttg
    10141 tgtgaccaga cggaaggacg aatgttgttg aggttttaga accatataaa acagcatgtt
    10201 tctcaaattt ttaggataag ggaaattgct agcagtttgt tttagaacca tataaaacag
    10261 catgtttctc aaatgagtac tttaaattaa ttgatggcgt tcctgcttta gaaccatata
    10321 aaacaacatg gatttaaaac aagaagtaag tcagttctcc ttttacttta gtaaggaaaa
    10381 ataaagtatt ctcaataaaa cttaaaataa gcaaaccttt
//
LOCUS       DAZUKT010000092        10396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000092 DAZUKT010000000
VERSION     DAZUKT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.094"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..325
                     /locus_tag="ACG816_002476"
     CDS             <1..325
                     /locus_tag="ACG816_002476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4921318.1"
                     /translation="KEVKKNIKENSIEIIESKNKLIYLKNNTNRDEGLVLLLISLIGC
                     LISPLGLIIAPVVLKRNKATNTFYKGVIAASVICFTVSLFVSYGHFSNIFHWGGSTNV
                     EYIEN"
     gene            complement(322..786)
                     /locus_tag="ACG816_002477"
     CDS             complement(322..786)
                     /locus_tag="ACG816_002477"
                     /inference="COORDINATES: protein motif:HMM:NF037267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5626 family protein"
                     /protein_id="HFS4921319.1"
                     /translation="MRQVLLVVTLCLSFFLFEVNTVEAYVANNGVYYNLIQGGTQEFM
                     EVDEEGNPILIRVEEVPGLSLFSVNNGNYKISGSKFGMWKANYYVTVSNNQFIRAYSP
                     SAVAVTGSFTSTRLSLVSSKKASYTLEWKAGILSSNSYLDATLTDNSINITY"
     gene            1700..3241
                     /gene="istA"
                     /locus_tag="ACG816_002478"
     CDS             1700..3241
                     /gene="istA"
                     /locus_tag="ACG816_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010013312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS4921320.1"
                     /translation="MFRYRRILEMHSEGISIRSIAASTGNSRQKITEVIRKAEEKGIA
                     RALSEEMTDQWLGEFLFPEKQLEANGRHLPDFDKIHKELAKPNVTLTLLHQEYAVECR
                     MSGKIPYAYRTFCDYYNAFARKHKATMRIRRKPGEIMEVDWAGSPAYVIDRDTGEKVK
                     AYVFVATLPCSQLSYAEAFLTMKSVAWITAHNHAFEYFGGSTDIIVPDNLKTGVKKNE
                     GIDVILNESYRELGEHYGSIILPARVSTPKDKPSVEGSVGVISTWIIAALRNTHCFTI
                     EDLNREIRMKLNEFNERPFTKKPGSRIRAFEEEEKFALNPLPLAPYKMSVWKKAKVQL
                     NYHVSVESMFYSVPYEYIQQNIDIRMTENLIELYFNSMRIATHKRLYGKFGQYSTVRD
                     HMPDNHKMYVDHTPESSMEWAETIGSSTVKVVQHLLDRYQNEKQALKSIMSLKSFGKK
                     YSKNEIETACSTALNLASLPSVKIIQTILKGNKQKRATSQSHIEDTNIEKHGFTRGAS
                     YFGGK"
     gene            3241..3993
                     /gene="istB"
                     /locus_tag="ACG816_002479"
     CDS             3241..3993
                     /gene="istB"
                     /locus_tag="ACG816_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS4921321.1"
                     /translation="MMLNEQTEVKLAELKLSGMLESYREQMTNKEYQSMSFEDRFNLM
                     VDLEHSRRKNNKLQRLIKSANFRNASASIEDIEYHPDRNLDKNLILKLASGTYLEEHH
                     NIILMGASGNGKTYIANAFGIQACRQYYNVKYIRLPELLDELTISKNQADGSFRKIIN
                     RYKKVQLLIIDEWLLTNLSLENSTFLLEIIESRLKTASTIFCSQFHPEGWHEKLGNPQ
                     IADAILDRIIHDSYQILVDGKVSMRERYGIRN"
     gene            complement(4176..4751)
                     /locus_tag="ACG816_002480"
     CDS             complement(4176..4751)
                     /locus_tag="ACG816_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921322.1"
                     /translation="MKNKFKYLFTILALLPITTSTLITPTIAKASEKQEDSVSYEIVK
                     NDLYIQFEEELAELIASNQSTVSVPDLFSTQSVLSTSSQDNSISEEQFFSLVEKYDGA
                     ELTAVVNSQDAFTTKFVNGGVNSYATSYRGLNALRSELKYNAGALKVGGTLVGTAGGL
                     LGVLLGMVTGDYLSGHCDDAAAGVSAFIQAG"
     gene            5539..6111
                     /locus_tag="ACG816_002481"
     CDS             5539..6111
                     /locus_tag="ACG816_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HFS4921323.1"
                     /translation="MNKAIFWDLLGTLGGDSRTLINQNFNFFKDTIPALKKAAQSHYL
                     NIIITNQSHISHGRITMAEYTDALESLINELEKNDIQITDVYTCPHARKDNCDCKKPQ
                     PTFVNEALKKYDLNPSKCFVVGDSGKNDMMLAKNAGINSVLLLTGEGIDSLTKYRHLW
                     SETKPTFIAENSLDAINKIHMYADGDSSDS"
     gene            6304..6867
                     /locus_tag="ACG816_002482"
     CDS             6304..6867
                     /locus_tag="ACG816_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4921324.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(7146..7433)
                     /locus_tag="ACG816_002483"
     CDS             complement(7146..7433)
                     /locus_tag="ACG816_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4921325.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(7423..7752)
                     /locus_tag="ACG816_002484"
     CDS             complement(7423..7752)
                     /locus_tag="ACG816_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4921326.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            8057..8386
                     /locus_tag="ACG816_002485"
     CDS             8057..8386
                     /locus_tag="ACG816_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS4921327.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(8673..9158)
                     /gene="vanZ-A"
                     /locus_tag="ACG816_002486"
     CDS             complement(8673..9158)
                     /gene="vanZ-A"
                     /locus_tag="ACG816_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS4921328.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9311..10222)
                     /locus_tag="ACG816_002487"
     CDS             complement(9311..10222)
                     /locus_tag="ACG816_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFS4921329.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3489 a         1844 c         1728 g         3335 t
ORIGIN      
        1 aaaagaagtt aaaaaaaata taaaagagaa tagtatagag ataatagaat ctaaaaataa
       61 attaatttat ttaaaaaata atacaaaccg agatgaaggg ttagttttat tgctcatttc
      121 cttaatcggc tgtttgattt ctcctcttgg actaattatt gcgcctgttg ttctgaaaag
      181 aaataaagca acaaacactt tttataaagg agtgattgct gcttcagtca tctgttttac
      241 agtaagcctg tttgtctcct atggacattt tagtaatatc tttcattggg gcggctccac
      301 taacgtggaa tatattgaga actaatacgt aatattaatg gaattatctg tcagtgtagc
      361 atctagataa gagttactag ataaaattcc agctttccat tctaatgtgt agctagcttt
      421 tttgctggag acgagagaca gtctcgtgct agtaaaggat ccggtcactg cgactgcaga
      481 aggggagtag gccctaataa attggttatt cgagactgtc acatagtaat ttgccttcca
      541 cattccaaat ttagatccag atattttata atttccgttg ttgacggaga acagggagag
      601 cccgggaacc tcttccacac gaatcaagat aggatttcct tcttcgtcta cctccatgaa
      661 ctcttgcgtg ccaccttgga ttaagttata gtagactcca ttatttgcca catatgcttc
      721 tacagtgttt acttcaaata aaaagaaaga taaacataaa gtaacaacca ataaaacttg
      781 tctcaaaaaa tccctcctaa aattaatata ctcatagttt aatatatacg ggtttaatta
      841 gtaagagaac attttgtgaa cattcttata ttgaaacggc tctctctaca aatggacgca
      901 tatataagtg tatctgggtt agtaaacatt cactgttacc aactctactt actaaacaga
      961 taaaatatca acgaaataaa aaaaccaaat caactaattt tttactaata acgtagataa
     1021 taaaatcaaa aagcaaattt gagcggttgc aactataaag tgacctgatg taaaccataa
     1081 gatgcttaat taagctatac ttagatgaga cactatactt aggaagaact tagattagca
     1141 tagcttccta gaacaaaaaa atacgagagg aagattcttt tatgactaat aaatatctat
     1201 ctgtatttac ttttttggtt ggcttgtttt ttgttattaa tagtgtagca agttctgatt
     1261 ttgatcttag taagattact cttttaactg tactaccaat aataataggt agtgtgttaa
     1321 ttcttttttc tgtgacttcc ctttttagac agcgaggtaa ttccaaaaag cattagcgaa
     1381 aaagataaaa gcagttttct ctttacccaa gagagaactg ctttttatca tctccacatt
     1441 tcttgcttaa ttaattacag cttcataaat actatcaata tggctaatag ggaaaccttc
     1501 agtaagggtt accctagatc ctcctctagt gtaaccagtg attacggcat cgaaaatgta
     1561 ccatctctgc aataattttt accaggctcg gcaaagaaat gtaccagtaa gtgcaaagca
     1621 gtgtaccaca aatacctctt gtataatgta ggtgcacacc ttagggtgtg acatttattt
     1681 tacaggaggt atttttatta tgtttcgtta tcgaaggata cttgaaatgc acagtgaagg
     1741 aattagtatt cgcagtattg cagcaagcac ggggaactca cgtcagaaaa ttactgaggt
     1801 tatccgaaaa gctgaggaga aagggatagc gagggcgttg tcggaagaaa tgaccgatca
     1861 atggctaggc gaatttttgt ttccagaaaa acaattagaa gccaatggac ggcacctgcc
     1921 tgacttcgac aagattcaca aggaattggc caaaccaaac gtgaccttga cgcttcttca
     1981 tcaggaatac gcagttgagt gtcgcatgag tgggaaaatc ccttacgcct atcgtacatt
     2041 ttgtgactat tacaatgcgt tcgcgcgaaa gcataaagcc actatgcgca tccgtcggaa
     2101 gcctggtgaa attatggaag tggattgggc tggatcgcca gcttatgtca tcgatcgaga
     2161 tacgggtgaa aaagtaaagg cttatgtctt tgtggcaacc ttaccgtgca gccaattgtc
     2221 ttatgcggaa gcattcctaa cgatgaaatc ggtggcttgg attacggcac ataaccatgc
     2281 atttgaatat tttggcggtt ctaccgacat aattgttccc gataatctaa aaacaggtgt
     2341 aaaaaagaat gaaggcatcg atgtcatcct taatgaatca tatcgagagc taggggaaca
     2401 ttacgggtcc atcattttac ctgcccgagt ttctactccc aaggataagc ccagtgttga
     2461 aggcagcgta ggcgtcatct ccacttggat tatagctgcc ttacgcaata cgcattgttt
     2521 tacaatcgaa gatttaaatc gtgaaatcag aatgaaatta aatgaattca atgagcgacc
     2581 gttcactaag aaaccaggat ccaggatcag agccttcgag gaagaagaaa aattcgcact
     2641 taatcctctt ccactggcac cttataagat gtctgtctgg aaaaaagcaa aggttcaact
     2701 aaactaccac gtctcagttg aaagcatgtt ttattccgtt ccatacgaat acattcaaca
     2761 aaatatagat attcgaatga cagagaatct aattgaacta tattttaatt ccatgcgaat
     2821 cgcaacacat aaacgtctgt acgggaaatt tgggcagtat tcgactgtca gagatcatat
     2881 gccagataat cacaaaatgt atgtggacca taccccagaa tcctcgatgg aatgggctga
     2941 aaccatcggt tcctcaacag taaaggttgt tcaacacctc ttagaccgtt atcaaaatga
     3001 aaaacaagcc ttaaagtcga tcatgtcttt gaagagcttt ggtaaaaagt attctaaaaa
     3061 tgaaatagaa acggcctgtt caacagcttt aaatctggct tctttgcctt ctgttaaaat
     3121 tatccagacc attctaaagg gcaacaagca gaagcgagca acaagccaat cacatattga
     3181 agacactaac attgaaaagc atggattcac tcgcggtgcg tcctattttg gagggaaata
     3241 atgatgttaa atgagcaaac agaagtaaaa ttagctgaat tgaaactttc aggcatgctt
     3301 gaatcctatc gtgaacagat gacaaataaa gagtaccaat ccatgagttt cgaagaccgc
     3361 ttcaacctca tggttgatct ggagcattcc cggcgaaaaa ataataaact acagcggttg
     3421 attaaatctg caaactttag aaatgcctct gccagcattg aggatatcga ataccatccg
     3481 gaccgtaatt tagataagaa cctcatttta aaactagcca gtgggactta tttggaagaa
     3541 caccataaca tcattttgat gggcgcttca gggaatggga aaacgtatat cgccaatgcc
     3601 ttcggaatac aggcatgccg gcaatactac aacgtgaaat acatccgact ccctgaacta
     3661 ctagatgaat taaccatctc gaaaaatcaa gcagatggta gttttcgaaa aataatcaac
     3721 agatataaaa aagttcaatt actcatcatc gatgaatggc tattaaccaa tctttcgtta
     3781 gaaaattcca cctttctatt ggaaataatc gaatcccgtc ttaaaacagc atcaactatt
     3841 ttctgttctc aattccatcc agaaggatgg cacgaaaagc tcggcaaccc ccaaatcgct
     3901 gatgccattc tagataggat catacatgat tcttatcaaa ttctggtaga cggtaaagtt
     3961 tctatgcgtg aacgttacgg catccgtaat taaatgagtc attaaaataa attaagagcc
     4021 actccatgat tttcatgagt ggctcttagt tgaccttgtg gtcattattg tcgcttttat
     4081 tatttgaaat ggtaaccgcg tcgcacttac tggtacattt ctttgcactt agtggtaaaa
     4141 aacaatgctc tagtggtaca ttttcgatgc cgtattcaac cagcttgaat aaaggcacta
     4201 acacctgctg ctgcatcatc gcaatggcca gataaataat cccctgtaac catgcctaat
     4261 aatactccta acaatccccc agctgtgcca actaaggtac ctcctacttt caaagcacca
     4321 gcattatact tcaactcgct acgcaatgca tttaatcctc tgtaagaagt agcatatgag
     4381 ttaaccccac cattcacaaa tttagttgtg aaagcatctt gtgaattaac aacagctgtc
     4441 aattcagctc catcatattt ttcgactaaa gaaaaaaatt gctcttcact aatagaatta
     4501 tcttgactag aagtagataa tactgattgt gtagaaaata aatcagggac tgaaacagtt
     4561 gattgattgg aagcaatcaa ttcagctaac tcttcttcaa actgaatgta aagatcattt
     4621 tttacaattt cataactaac agaatcttcc tgtttttcag aagctttggc tattgtagga
     4681 gtaattaagg tcgatgtagt aattggcaat aaggctaaaa tagtaaataa atacttaaat
     4741 ttatttttca ttttttatcc ttctttctat tttttctact tttaatacca caacttgaaa
     4801 aataaaaaat ctaagcaaga gtaacatctc ttacgtaatg attttgcaat agtttttcct
     4861 cctctcacaa tctattatta tgtttttcga gctgtttcaa aagcactttt ctatttacat
     4921 ctgggaaact aatcgttgct tttataaaaa aagatgatag cagaaaaaat tgaacttttc
     4981 tttactataa ggtatttata aaatcatctt cataggtcaa agtagtagta ttaatctcaa
     5041 ctttgataag ctggtgtata tcaattttct ctttaatatg tacaatcatt aaaaatagat
     5101 ttttttacta tttatggttt caattaacaa aagaacctcc acactaaatc actataaatt
     5161 tattatcacc ttagtttttt atgtgctcct agcatggaaa tcccatcaaa aaagtaaggt
     5221 ttgtattcaa atgtttttcg ccaacgaggg tatcttcttt tttacactct ttgctgtatc
     5281 aaaatataac tgaaattcta agtctcttct atatcagtga ttatgtgatt cgaaatatcc
     5341 attccctcaa aagaagttca tatagaaagt gaatagagag tggctcctgt aaatatgata
     5401 tagttaaact ggcaaaataa aatatgcttt ttcaaaaagt aatatttttg ttttaattac
     5461 gaagaactac ttttaaatac tgtaagcacc ttggcttgtt agttaactat tcttaaaatg
     5521 agagggagac aaatatccat gaataaagct atattttggg atctgttagg tacgctggga
     5581 ggggatagta gaacactgat taatcaaaat ttcaattttt ttaaagacac aataccggcc
     5641 ttaaaaaaag cggcacaaag tcattatcta aatatcatca ttacgaatca aagtcatatt
     5701 tcacatggac gtattactat ggcagaatat actgatgcct tagagagctt aataaatgag
     5761 ttagagaaaa atgacattca aataacggat gtatacactt gccctcatgc tagaaaagac
     5821 aattgtgatt gcaagaagcc tcaacctaca tttgtcaatg aagcattaaa aaaatacgat
     5881 ttaaatccaa gtaagtgttt cgtagtaggc gattccggta aaaacgatat gatgttggcc
     5941 aaaaatgctg gtataaacag tgttttactt ttaacgggag agggaattga tagtttaacc
     6001 aaatatagac atctgtggag tgaaacaaaa cctactttta ttgctgaaaa cagtttagat
     6061 gctataaata aaatacatat gtatgcagat ggagactctt ctgattcatg ataccagaag
     6121 agtctccatt tattactaaa aaccgtatca aaaacaatcg attatgagac aaaatgaaat
     6181 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
     6241 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
     6301 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
     6361 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
     6421 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
     6481 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
     6541 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
     6601 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
     6661 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
     6721 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     6781 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     6841 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     6901 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     6961 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     7021 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     7081 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     7141 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     7201 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     7261 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     7321 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     7381 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     7441 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     7501 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     7561 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     7621 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     7681 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     7741 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     7801 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     7861 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     7921 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     7981 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     8041 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     8101 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     8161 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     8221 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     8281 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     8341 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     8401 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     8461 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     8521 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     8581 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     8641 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     8701 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     8761 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     8821 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     8881 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     8941 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     9001 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     9061 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     9121 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     9181 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     9241 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     9301 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     9361 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     9421 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     9481 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     9541 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     9601 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     9661 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     9721 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     9781 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     9841 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     9901 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     9961 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
    10021 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
    10081 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
    10141 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
    10201 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
    10261 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
    10321 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
    10381 gaattttcat aagtga
//
LOCUS       DAZUKT010000093        10316 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000093 DAZUKT010000000
VERSION     DAZUKT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.095"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..903)
                     /locus_tag="ACG816_002488"
     CDS             complement(70..903)
                     /locus_tag="ACG816_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4921330.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1207..3372)
                     /locus_tag="ACG816_002489"
     CDS             complement(1207..3372)
                     /locus_tag="ACG816_002489"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4921331.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3632..5926
                     /locus_tag="ACG816_002490"
     CDS             3632..5926
                     /locus_tag="ACG816_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4921332.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5919..6584
                     /gene="pgmB"
                     /locus_tag="ACG816_002491"
     CDS             5919..6584
                     /gene="pgmB"
                     /locus_tag="ACG816_002491"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4921333.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6577..7584
                     /locus_tag="ACG816_002492"
     CDS             6577..7584
                     /locus_tag="ACG816_002492"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4921334.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7606..8625
                     /locus_tag="ACG816_002493"
     CDS             7606..8625
                     /locus_tag="ACG816_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4921335.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8906..10273)
                     /gene="nhaC"
                     /locus_tag="ACG816_002494"
     CDS             complement(8906..10273)
                     /gene="nhaC"
                     /locus_tag="ACG816_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4921336.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3197 a         2241 c         1909 g         2969 t
ORIGIN      
        1 tctccaaacg gtataatcgt ttggagagtt ttattttaat cagttttttt atgttatatg
       61 ttatattttt tattcgttca catctaattc gatttccacc ccgtagtgat cactgatgat
      121 tggactatcg atcccatcaa aaatccgttg atatttttta acctgcattc cttcagggac
      181 gaaaataaaa tctatacgca atttttctgt attttgttcc caaccatcta ttttcttttc
      241 cacagtagct gcttttcctt tttcattcgc gacaacatac gaatcttgaa gaagaaggca
      301 actttctctt acagtataat aaccttgaga gtctactgga ttattgaaat cacctaggaa
      361 aaaaagtggt acgcgagttt ccaaaaagta gttttccaag tttttccact caaaataaaa
      421 gccacttttt ttatctgtcc accaagaaaa gtggcagctc gagacaaaaa tgtcctgatc
      481 atctatcttt gttttcccaa tcaagatctt gcgtgtctga taatcttctt tcttttgaaa
      541 ttgggagacg gtaaggacct gactttccaa tgtttttttc gtaagcagtg catttccttc
      601 ttcatattta tcataaccga tgtggctcat ttcccaactc caaaaatact cttttccgtg
      661 ttcctttaaa agctgaacta aacggtaggc aaaattatct ttacggatag gtacttgatt
      721 ctttaccgga cagaacttca ttagcttcgc aggttctact tcttcagatt ccagtaattg
      781 gttgatttct tgtaaagcaa tcacttcata atcttctaac aggatcttat ctgctaattc
      841 ttgcaatttt ttctctggtt ctggctcaag ccagctatgc gtgttgagtg tcatgaattt
      901 cattggtttt ctccttgtct tcatttcgaa acaaaagggt tgtgacaaaa tttttgtcac
      961 aacctctcca gcaaatagtg ttctcctgct aactcctcag taataagctc aatggaccag
     1021 gagctatttt ttgtttggct gtttttatca cttggtgcag ggcagctttt tcacgacctc
     1081 tccactaaac agtattcaaa ctgctgcttc tcggtaataa gcccaaccaa acgaaaaata
     1141 ggaggagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
     1201 ctctccttac attttaatgg agccgatcac tttaccagct gtcactgtac ctaattgctt
     1261 gatcactact tcttctactt tatcgtttgt aaatacgatc atgatcgttg gatctttttc
     1321 ttcgttttgg atcacatcaa gatccatctc agctaatttc gttcctgttc ctacttcttg
     1381 gccttctttg acagcaatct caaaagctgg ctgattcatt tggactgtat cgattcccat
     1441 atggatcaaa acttcgatac catctttcgt ttccagccca attgcatgtt tgctagggaa
     1501 aatacttgtc acttttccag cgatcggcgc aacaacttct cttgatgttg gaataacagc
     1561 aaagccgtca cccatcattt tttgtgagaa taccggatct tttacttcag ataaaggaat
     1621 cacttctcca gaagctgcgg aaactagttc ttgttctata cctaagtatt gcttactacc
     1681 tgcttgtcca tttcctgtag aaaccatttc tggctctgga atctctgctc ctgattgcag
     1741 cagatcttcg atatctgatt tcaagacatc tgcttttggt ccataaactg cttggactcc
     1801 attgtctttg acgatcaatc ccatcgcccc tgctcgtttc caggcttctt cagtccctac
     1861 tttttctcga tcatttacac ttacgcgtaa ccgagtcata caagcatcga catctttgat
     1921 attttctttt cctcccagca aatgaatgat ctggaccact tgctgatctg ctgttcctgt
     1981 tccgctgtta gatacaacgg tttcttcact gtcattgtcg taatttccgt tacgtcccgg
     2041 tgtagcaaag ttgaattttt tgatcaagaa atttgctaag aaatacgtaa caacaccaaa
     2101 agcgatcaca cacaacacga agttgataag atctccacct agacccgctt tgatagctaa
     2161 cggtgtccga gtcagcaatt cgatattccc aaatgaatgg acccgcaatg gcaagatatc
     2221 tgccatcgca aatgcagccc cttggatcac tgaataaatg acatataatg gcacagcagc
     2281 aaacataaac atgaactcca atggttctgt tacacctgtt agaaagacag ccaacgctgc
     2341 tgaaaaatac attgatttat atcttgcttt cttatcagga tcgacattac gatacatcgc
     2401 aaaagccatt cccatcaaga tacctgaaga accaatcatt tgtcctactt tgaaacgagc
     2461 cggcgtccag ttatgcaaca caaactcata ttgcgacata tcccctgcac ctttaagatt
     2521 cactaaatca gtggcccaag ccaaccataa cggatcttgc ccaaatactt gtgtccccgc
     2581 ctgtgcgcct gataaaatct catacgatcc gccaagctgt gtatagttga taggaatcgt
     2641 cagcatatga tgcaatccaa atggcaatag taaacgttct aatgttccaa ataaaaacgg
     2701 tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat aaaccaaaat tattgattcc
     2761 agcctgtacg tttggccaca ccacagcaag aatcaatgcc acgattgttg accataagat
     2821 aacaacaaac ggaacaaagc gctttccatt gaaaaatgac aaagcatccg gcaattttct
     2881 ataattatag tacttattat aagccattgc cccgacaaat ccagcgatga tccctacgaa
     2941 tactcccata ttcaaagctg gtgcttccaa tacacttgta aaaaatcctt taaccatgat
     3001 cttcgtaccg aacagagtgt gggtaaatgc atcttcattc gcaagcatgt cacttgtgac
     3061 accaaagata gccccagtga tccgattgat taaaataaat gagatcccag ctgcaaatgc
     3121 tccgcctgct ctttcttttg cccaacttcc tcctattgct aaagcaaata aaatatgaag
     3181 attaccaata attgcccaac caatattttc aattactccg cccgttgtga caagcggcgt
     3241 ccaatcagga ttgatcaagg gaattgattt ccctatactg atcatcaacc cagctgccgg
     3301 cataactgct accacaacca ttaaagcctt tccaaatttt tgccaaaact caaaactaaa
     3361 tatctttttc attccgttta gctcctttac gcaatcggtt tcgttaaacg tattatagcg
     3421 tgcgttttct tttttgtaaa tgctttattt tttatttttc tgtctatacc attctataaa
     3481 acctataata cgtgtgttcc cttctataat tttctcctaa aaatttattt ttttaagctt
     3541 ttttctatga aatattttct tttgtctatt gacttgtgca atcggttgcg tttattatga
     3601 acgtataaaa cagctcatta ggaggagaaa aatgaaacag atcaaacgac tatttgctat
     3661 tgatccatgg aaaatcagca cgacacaatt agataaaaaa aatcttcgac tgcaagaatc
     3721 tttaacaagt attggaaacg gttatatggg gatgcgcggg aactttgaag aaacttactc
     3781 aggggaccac catcaaggta cttatttggc gggtgtctgg tatcccgata agacacgtgt
     3841 cggctggtgg aaaaatggtt atccagaata ctttggaaaa gtgatcaacg ccttgaactt
     3901 tatcgcgata gatatttcga tcaacgatca gctgatcgac ttggcttctt tggaaccaga
     3961 agacttttac tgggaattag atatgaaaaa tggtgtatta tctcgtacat tcacaatcac
     4021 tacttctact aataaagtac gcatttcctt cgaacgtttt ctgagcattg tcaaaaaaga
     4081 atcagcttat atctatttaa ctatagaaat gcttgaaggt agcggaacag tcaaactcgt
     4141 atcaaaattg gatggaaatg ttcaaaacga agacagcaac tatgatgaac atttttggaa
     4201 tgagattgat cgtggaacta tagaagaaat cggctatttg acgactaaaa ctattccaaa
     4261 caactttggg attgaacaat ttactgttac tgcagcaatg cgccatttcg ttaatggaaa
     4321 atcagtcatc cctgactatc acgaagatac tttagcatta catggttgct tgtctgcaga
     4381 attatctcaa ggtgagacat ggtcagtgga aaaagaaatt atcgttctta ctagtagaga
     4441 tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa ttaacgaacc agacaaacaa
     4501 ttatatccaa gcaaaagaag agcaaacagc ggcttgggca gaacgatgga agcttgccga
     4561 tgtggtcatc gaaggagacg aagaagcaca acaaggtatt cgattcaatt tgttccaact
     4621 attctctact tactacggcg aagatgaacg attgaatatc ggccccaaag gattcacagg
     4681 agaaaaatac ggcggagcca cttactggga tacagaagcg tatgctgtcc ctctttatct
     4741 tgctttagcc aaacctgaag taactaagaa tctgctgaaa tatcggcata atcaactgcc
     4801 gcaagcaatc cacaacgctc agcaacaagg attaaaaggc gcgctttatc ctatggtcac
     4861 cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca ttcgaggaga ttcaccgaaa
     4921 tggcgcgatt gcctatgcaa tctacaatta tacgaattac accggcgata cggaatattt
     4981 gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc ttctgggcag accgtgtcca
     5041 tttttcaaaa cgagcaaata aatacatgat ccacggtgtg accggcccaa acgaatatga
     5101 gaacaatatc aataacaatt ggtatacaaa tacgatcgca gcttgggtct tgcgttacac
     5161 gcgagaaaat tatttaactt atcaaaaaga agctgctatc actatcagcg aagaagaact
     5221 agccaaatgg aaagatatcg cagagaacat gtacttccca gttgacgaag aattaggaat
     5281 ctttgtccag catgacacat tcttagataa agatctcatg cctgtatccg atattcccac
     5341 aagcgatctt cctctgaatc agcattggtc ttgggacaaa atccttagat cttgcttcat
     5401 caagcaggca gatgtactac aggggattta tttcttcaat gatactttta caaaagaaga
     5461 aaagcaaaaa aactttgatt tctatgaacc aatgactgtc catgagtctt ccctttcgcc
     5521 aagtatccat tcgattttag cagcagaatt aggaatggaa agcaaagctg tagaaatgta
     5581 ccagcgaaca gctcgcttgg atttggataa ttacaataac gacacagaag acggcttgca
     5641 catcacttcc atgactggta gctggttgtc gattgtccaa ggatttgctc aaatgaaaac
     5701 tgcccatgat cagttgagat tcgcaccatt tctgccaaaa acttggaaag cttattcttt
     5761 ccatatcaat tatcgcggac gtttattgtt catctcagtc acagatgaac aagttacttt
     5821 cgagttatta gaaggttctc ctctagaaat ttacgtctat gaacagtctt attctttagc
     5881 ggatcgtcta gaaataacgc taagaaagga ggaagcaaat gtttaaaggg gtattgtttg
     5941 atttagacgg tgtcattacc gatactgctg aattccatta tcgtgcttgg cgaaagcttg
     6001 gtcaggaaat cggcattacg atcgaccaca cattcaacga acagctaaaa ggcgtcagcc
     6061 gagaagactc actctctttg cttttagctt atggcggaaa agaacactct ttttcaaagg
     6121 aagaatttgc agaattagcg aaaagaaaaa acgattatta tttagagatg atccagacga
     6181 tcgaaccaaa agatgtcttc cccggggtcg tgcctttact ggattcttta aaagaagaaa
     6241 aaatcaagat tgctcttgct tctgcaagta aaaacgggcc attcctactt gaaaaaatgg
     6301 gtcttacacc ttactttgat gctattgccg acccggcaga agcaacaaac ggaaaacctg
     6361 cgccagatat cttcctcctt gcagcaaaag ctgtcggact aaaagcagaa gaatgtatcg
     6421 ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc aagtggcgct caaccaattg
     6481 gcgtgggacg tgctgaagac cttggagagg agatcccgct tgtccctgat acgacttttc
     6541 ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg ataatccaga gatctccgtc
     6601 gtaccagttc cagagactga aataaagaaa atcacattga agaacgacta cttagaagtc
     6661 gttcttctca attacggtgc ccgcctgcac caaatcttcg caccagacaa agaaggaaaa
     6721 tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg ttctaacaga caaaagcttt
     6781 tttggagcaa ctgtcggacc tgtagccgga aggatccggg atgcttcttg gggtgagcat
     6841 agattggaga aaaactgcgg atcacaccat atccacggtg gcacgaaagg ctggtctttc
     6901 caattttggg aagtagaagt tttcaaaact ccatcatcaa taggtgtggt cttttactta
     6961 aaagatgaat tttctaccta tccaggacca atcactgcta cgatcactta ccgtttaaca
     7021 aaaaacgaat tagagatgac aacctctgct agtagccttg ttgaaaccat ctgcaatccg
     7081 accaaccatg cctacttcaa cctttcagga aatggaacaa gagacatcta tgaacatcag
     7141 ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag aaaaactgcc tactggaaat
     7201 tggacaaaaa aagaaaaact gccaatcgac tttagaaaat ccccaacact gcaagagatc
     7261 cttgcatgtt atccagatgg actagatgat gtatttcttt tgcatcatcc taggctttcc
     7321 aaaacttcct tacaattatt cgagaagcat tctggtcgcc aaatgactat agcaacttct
     7381 aataaaagta tggtattatt ttctacgact ggattcgaag ctgatttctc tgtcaatgga
     7441 aaacagatgc attcaaacta cggattagca atcgaaccgc aagaaatccc tgatatcgtt
     7501 catttcccca aatttggatc gatcaacctc catcctggtc aagaacggat cagtcagacg
     7561 atttatcggt tctctgcaca ataacaagag aaaggtgttt ttatcatggc tattaccgta
     7621 aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg tctctcgtgt catcaacgat
     7681 catcctagta tttccgagac aacaaagaag aaagtccgaa aaattatgga tgaactaggt
     7741 tatgttccta acatgactgc ccgcaatcta ggaaaacgca tttccagtgc catcggcgta
     7801 atcttacccc ctcttgattc aaaagagcgg atcggtaacc cattttatct tgagatcatg
     7861 gaagcaatca acgaagaagc gcggaattat gatatgacta cagcaatcgc tactgctaaa
     7921 aattttgaat tgctgttaga gaacgtccag cgtatgcatt tacaaaaaca agtagatggt
     7981 tttattctag tgtattcaga tagtcaagat actgtcattg attatttata ccaaaaccac
     8041 atccctttca ctttgatcgg acagccttat catcatgaga acgaaatcgt ttatgtagat
     8101 aatgataacc agcttctagg taaacaagca acagaatttt tgatccagaa tggacataag
     8161 aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt atttcgaacg atatttcggt
     8221 tatcaaaaag caatgatgat ggctggtctt cctgcgttat ccagtgtagt actagagcat
     8281 ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa acgaagccac agcattggtg
     8341 gtcattgacg atatcttcgc ccttcgcacg atgcagttgg cgcaaatgta tggctatgtc
     8401 attccagaga cactctccgt cattagcttc aacaactcga tcttctcaac attaacacac
     8461 ccttacctta caagtatcga tatcgatatc tccgaattag gacgtgtcgc tatgcaaaag
     8521 ctgcgcgaat tgatccatga agaattgagc agtggcgtac gtttggtcat ccctcatcgc
     8581 ttgatcaaaa gagagacagt cgttccttta gaaaacccat actaaatagc tgactcacta
     8641 aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata ctacttctaa
     8701 cacattgttt atctgtaaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg
     8761 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
     8821 acaaaaattt aaaaagcaat tttcttgagt ttcttcttat ttcttggagt taaacgcttc
     8881 tgtcccagcc tcttttttcc ttccttcaat tttttacacg tcgacgattc aataaaaaac
     8941 cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa ggcgaatggc aagtaatgca
     9001 acgcgctaac tttcatcgtt cccataatga atgtgccgct gactccccac ggtacgagag
     9061 aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa ataggttttt gataagccaa
     9121 cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat gatggacaga tactgttctc
     9181 caacgatcag attgattccg atacagctca aggcagtaaa cccgactaaa cgagaaggtc
     9241 ggtccatctt ttcttttaat tcttgaacga tggtatcaac gatcagatat ttgatcagta
     9301 acccacctaa tgccaatgca agtaaaatca aactagcaga acctaacata ctcatgatcc
     9361 cgcctcgaga aagcagttca tctagctttg gatcaggtgt atttgcgaca taaccattca
     9421 tcaagatgtc tgctgctttt ccgaccgtca aacttttctc atggataaaa gaaagaacga
     9481 gagcagctgt cgatcccgct agtaatgtag ggatagccgg aatttttttg attgcccccc
     9541 caaataaaat aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt
     9601 tttggatcat ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg
     9661 tataaaaaat caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat
     9721 gttcgaatag attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg
     9781 agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga
     9841 atccaagtaa atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg
     9901 aactgccgac agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac
     9961 gcacagaaac aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc
    10021 atgaagcaac taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga
    10081 ttcctttgat gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa
    10141 acaacaaaca catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga
    10201 tcagtactcc taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa
    10261 tatttttatt catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaa
//
LOCUS       DAZUKT010000094        10152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000094 DAZUKT010000000
VERSION     DAZUKT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.096"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(225..1007)
                     /locus_tag="ACG816_002495"
     CDS             complement(225..1007)
                     /locus_tag="ACG816_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921337.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1072..1908)
                     /locus_tag="ACG816_002496"
     CDS             complement(1072..1908)
                     /locus_tag="ACG816_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4921338.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1926..3335)
                     /locus_tag="ACG816_002497"
     CDS             complement(1926..3335)
                     /locus_tag="ACG816_002497"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4921339.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3349..5172)
                     /locus_tag="ACG816_002498"
     CDS             complement(3349..5172)
                     /locus_tag="ACG816_002498"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4921340.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6015..6341)
                     /locus_tag="ACG816_002499"
     CDS             complement(6015..6341)
                     /locus_tag="ACG816_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921341.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6387..6749)
                     /locus_tag="ACG816_002500"
     CDS             complement(6387..6749)
                     /locus_tag="ACG816_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4921342.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6786..7000)
                     /locus_tag="ACG816_002501"
                     /pseudo
     CDS             complement(6786..7000)
                     /locus_tag="ACG816_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7128..7463
                     /locus_tag="ACG816_002502"
     CDS             7128..7463
                     /locus_tag="ACG816_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4921343.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7539..9536)
                     /locus_tag="ACG816_002503"
     CDS             complement(7539..9536)
                     /locus_tag="ACG816_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921344.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            9656..>10152
                     /locus_tag="ACG816_002504"
     CDS             9656..>10152
                     /locus_tag="ACG816_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS4921345.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         3169 a         2109 c         1530 g         3344 t
ORIGIN      
        1 taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat atatcaacgt
       61 ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg ctccaaatcc
      121 gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag tacggtcata
      181 taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc aataactttt
      241 ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga ataatctttt
      301 ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt cactgcatta
      361 ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt agaaatctct
      421 gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt aggtggtata
      481 gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc agtatctact
      541 tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc cgtaactgct
      601 tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata ttgtccatct
      661 ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata accagttctt
      721 tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata gacatgtaaa
      781 agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga ccttcctata
      841 cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt aatctgccaa
      901 tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac cacttcactg
      961 gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga atcaggagcc
     1021 ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca ttcagtacac
     1081 ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg cacccatctc
     1141 ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa aacgctgctt
     1201 cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa tcattcgggc
     1261 aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt tatcaacctt
     1321 tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc ttaccgagag
     1381 catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa acgcaatgtt
     1441 tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt ggatagcata
     1501 gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca ggcgattttg
     1561 aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa ccaaggtaaa
     1621 aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga taattcgctt
     1681 gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat ccttttttag
     1741 agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg gaaccaaatc
     1801 gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt tgccttgctt
     1861 atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta gattcaccgt
     1921 tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa gctttctttt
     1981 tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat aaaaccataa
     2041 cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg taaataacca
     2101 aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt ggctagataa
     2161 tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg tgccccatag
     2221 ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct caatgttaat
     2281 cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata aggattttca
     2341 gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc tctagcggaa
     2401 acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata attaatcgca
     2461 ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat tttaggatat
     2521 aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg aactgctaca
     2581 tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc atcttctgga
     2641 ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt tcctttaaga
     2701 ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg gtgattaatt
     2761 tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat cgctccagga
     2821 tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt gtcaccatac
     2881 tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg atttaaccaa
     2941 ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac aatcggttcg
     3001 attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc cttttgattg
     3061 gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg aaaagctttt
     3121 aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa atccgaggca
     3181 acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga cggttgtttc
     3241 ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc ccataaaaac
     3301 ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt aactaatttg
     3361 catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg cgtaattttc
     3421 actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa tggcagcgaa
     3481 atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta caaatacttc
     3541 aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata attcaatacc
     3601 ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga cttctccctc
     3661 aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc ccaatagtcc
     3721 cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt ttaccggatt
     3781 ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag tatcgtcaaa
     3841 tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg taataattgc
     3901 gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta agccagggga
     3961 aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa ttgcaccacc
     4021 aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga gtgaaggctc
     4081 agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca actctttgtt
     4141 tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag aagccagcat
     4201 tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag cgattggcgt
     4261 cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca tcaaaaatgg
     4321 aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa gatcgttcag
     4381 caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta aaaataccag
     4441 catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa agggctctac
     4501 ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat aatctgcagc
     4561 taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct gtgaaatatt
     4621 tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat caaactttat
     4681 ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag caatagtatt
     4741 caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca agacggccaa
     4801 aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa aggtggtaga
     4861 aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg ggcccttttt
     4921 aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt cattgccaat
     4981 caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg ttttttctaa
     5041 ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg tggcacaatg
     5101 ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc ttcctacttc
     5161 tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca aaccacgccc
     5221 caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt aacatgccaa
     5281 taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc aacagggaat
     5341 ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga tcacatcaac
     5401 tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta actataccag
     5461 taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca tcaaaaatgc
     5521 atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat gaatttggtg
     5581 agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga tacttgtttt
     5641 tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata aaggtatcga
     5701 tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa ctgatttatt
     5761 tctaatctac atgtagtaag tatttcaatt ccaccaatta tcaataaaaa taccaaacat
     5821 atccataata attttttctt tttcatttct tttatattgt tataacagtg aataacataa
     5881 aaattcccga caatcattgt aatatttttt tgtcacctgt attagtattt aatttatagg
     5941 aaactataaa aactccctag tgaatttaca cctttgggag ttcctgtttg gacggtatgc
     6001 ataatacatt taaatcaatt taacattaac acctcagcat atggtatctc cacatatata
     6061 cggtctttct ttggatagta ctcaatattt atgttgatag cacttccatt gtctaatata
     6121 gctttagccc ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc atcacatgat
     6181 cgatttaagt tgtactgttc ttgtaaaatc tgtgttacca atggtttcga tgacttctct
     6241 aatccagatg tacctgtcat ttttgaaaaa atgataccta taatagccaa gacaacgata
     6301 actataatga taaacttctt gttcatattt actttattca actttttatc tcctatctcc
     6361 aaaataattt gctcttatcc ctcttatcag tacattgaca tccctaaaag tacaccaaag
     6421 attgctccag ctacccctgc tatcatcaaa atcgtaccca tttgttcatc tttatctcga
     6481 tataagtagc ccataataac agcaacagca ccaaatacaa caggaataaa tgctaacgct
     6541 actaccatag aaatccaacc aataatcaaa tacagcttcc ctgtctcttg tccatgtacg
     6601 atttgttcct tcatttcatt aattgtctgc tcagtacttt tctttactgc gtctgctcca
     6661 gttgtctcat ctgtttcttg tagtgctccg caaaactggc aatacttact ttcatctgtt
     6721 atttctttgc cacattttct acaatacatg tcttctcctc ctaaaaacat tcaattgttt
     6781 attagttaac tgtttacttt attaaataca tcagaaaaca gctcattcat tggtacactt
     6841 aatatctcag aaagcatctt catttgatag ggtttaggaa tcgttctccc tgtttgccac
     6901 gaacttacac attgctgtgt aacattcatt tttttgctaa ctccgtttga gaaagtcctt
     6961 tttcttttct gtatcggctg agattactta cttttttcat gattctcccc tcctgtttta
     7021 ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaaactt caaataccat
     7081 tacaaataca acaacttgtt gtactatact gagatggggg tgtattaatg aaacagaaac
     7141 aatatgcaaa ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa aaagaattag
     7201 ctgaaaaaat cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagagc
     7261 ctaacctcga tagcttaacc gctctttcta gagccctagg aacccctgta agccattttc
     7321 tagacaatgt tgtcgtagat tatgaggaag agtttttggc tctctatcgt tctcttagca
     7381 ctgaagatgc agttcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac
     7441 gcaatcagct attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta
     7501 ttgagatgaa ataagattaa gagctatgga taacctgtct agtttaatac aattgggtct
     7561 tttctcttcc ttactctccg tccatcatct cttgttacac ttgtaaattg ataaagagga
     7621 actaattcaa ctcctgataa tatcaattct tttattactt catctatccc tgctcctttt
     7681 gctaggtaga ctaaaaaact actacttgct ttcacattgt atagtctctt aatatcctta
     7741 gaagctcgtt caagagtgaa tccttgttta acaaagtagc ctctgttttc ttttaaagca
     7801 ttttccacgc tgttattccc aacattacgt agacgttcac aaacagtgtt agtaagttct
     7861 ttctctgtca ttagatatgt tccttgccac gataagccct gtaaaacttc atgtgcttca
     7921 tctaaaatca attgtttttc cttgctaagt tctgatcggg aattttgagg aaatactctt
     7981 ttagcctctt tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta
     8041 aaatgaaatt tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt
     8101 gcattaaaaa cgtctaatcg atagacattg cttctcttat gattaaagtt tcctaacgct
     8161 ttctcttcct ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct
     8221 aattttttta tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg
     8281 tcataagttt taccatagta aaaactcatt tttttagcta attgttcccc cgacaaaata
     8341 caagtcacac tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag
     8401 acacgtcgca tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat
     8461 tcaagagtgt caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac
     8521 aagtctaggt tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt
     8581 agcatcacaa ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa
     8641 atggaatgcc cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac
     8701 agacatctta agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt
     8761 tcataaatct cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt
     8821 ttcatttctt ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt
     8881 tcatactctc tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc
     8941 ggaagctcag taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta
     9001 tatcctccaa aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc
     9061 tttttcaagt attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat
     9121 agaaagacaa tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca
     9181 aacgcagaat tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta
     9241 tcatctttac tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc
     9301 agaataatta ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg
     9361 cttggaataa atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct
     9421 ctatagttat ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc
     9481 tctaataatt tttctttatc tacataaatt ggaatattaa tcattgatgt ttccatttgt
     9541 gtttcctcga gagtataaaa tattttgtgt aaatgaaaaa atccatacaa aaaaggaagt
     9601 cccttctgta gaataaagtt aacgacaacc aattcacaga aaagaggact tccctatgaa
     9661 tgattttact acagaaattg tgcaaactct agtcactaaa ggcgatttaa atgaattatt
     9721 ccgttcgcac ttagaaaaag cgataaacac actcctacgg actgaattaa cggctttttt
     9781 agattacgaa aaatatgatc gcactggttt taattcaggt aattcgagaa acggttctta
     9841 ctttcgatca atcaaaaccg aatatggtga attaacattg gaaataccta gagatcgtaa
     9901 tggtgagttt aaacaacaaa ctttaccagc ctacaaaaga acaaacgata cattggaaac
     9961 cactattatc catttattcg aaaaaggtgt tacgatgtct gaaattgctg atttgatcga
    10021 aaaaatgtac ggtcatcact atactccaca aaccatgtcc aacatgacta aagttctgac
    10081 tgaagaagta aatgccttta aatccagagc cttaaatgat aagtatgtcg ctatttttat
    10141 ggacgctact ta
//
LOCUS       DAZUKT010000095        10131 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000095 DAZUKT010000000
VERSION     DAZUKT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.097"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..925
                     /locus_tag="ACG816_002505"
     CDS             146..925
                     /locus_tag="ACG816_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921346.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            971..1915
                     /locus_tag="ACG816_002506"
     CDS             971..1915
                     /locus_tag="ACG816_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4921347.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1931..2710
                     /locus_tag="ACG816_002507"
     CDS             1931..2710
                     /locus_tag="ACG816_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS4921348.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2722..3420
                     /locus_tag="ACG816_002508"
     CDS             2722..3420
                     /locus_tag="ACG816_002508"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS4921349.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3448..4212
                     /locus_tag="ACG816_002509"
     CDS             3448..4212
                     /locus_tag="ACG816_002509"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS4921350.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4256..5707
                     /locus_tag="ACG816_002510"
     CDS             4256..5707
                     /locus_tag="ACG816_002510"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4921351.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5739..6875
                     /locus_tag="ACG816_002511"
     CDS             5739..6875
                     /locus_tag="ACG816_002511"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4921352.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6905..7981
                     /locus_tag="ACG816_002512"
     CDS             6905..7981
                     /locus_tag="ACG816_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4921353.1"
                     /translation="MHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLG
                     GKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRI
                     AHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKFTLIPNAVDAK
                     KFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLI
                     IGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEA
                     QFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKAD
                     YNIQTAYKILESKYYELFELIQRG"
     gene            7987..9009
                     /locus_tag="ACG816_002513"
     CDS             7987..9009
                     /locus_tag="ACG816_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFS4921354.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9031..10086
                     /locus_tag="ACG816_002514"
     CDS             9031..10086
                     /locus_tag="ACG816_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4921355.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         3718 a         1430 c         2087 g         2896 t
ORIGIN      
        1 ggtatgatta tatagggaaa acttgtttac aaatatttct gtgaatatat tagtttaatt
       61 atgttttttt aaataaaaag ctgtcatttt tatgtaagtg aattatatct taatttatct
      121 atcatcaata cttaggagtg aatctatgga aaagccattc gtcaagtcat tgactgctgt
      181 ggcggtaggt gcaggtgcta ttgcgatctg tttgtttgga taccatatca ataatcagcg
      241 gcaacatcag caacggatca attatgcaga atctgccatc accaatcaaa aagacacctt
      301 gacttcttta tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa tttttctaaa
      361 ccctgaaata acagaagaaa caatcacaag tttgtcaaat aagctatcct ctgtcaaact
      421 ttcagcagat gattacgaca tcaaagaatc agaattgcct aaagaagctg ttgcaatcca
      481 agatgagaag aaagcaatcg tgacacagtt agatagtgca gaatcaaaac tgaagatcca
      541 gacagctgtc aacgatttat ttactaaaaa tgtatcaaac tggcagcaag ctgtagacga
      601 tgtcatcatc aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg aaaatatgag
      661 cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa tatctaggat tcgctgatat
      721 tcagatcgcg caagtcacgc aactagatca gttattcgat acgatgttaa aggatggtca
      781 agtgactgcc accgctactt acgatcaata tttgacggtg ctcagtcaaa tagagcagat
      841 ccggaatgaa aaaataagcg cagcatacgc aacaaaagca caaactgttg ctgagcaaat
      901 gggctatagc aatataagct attgacagtt aaaacgttga ttcaagcaag gttagcaaac
      961 ggaggaaact atggcaagac gaagaagaaa atcaagaggg aaaaaaatat ttctgattat
     1021 tttagcagtt cttatcgtat tagtattagc aattgtagga gtaggagcaa aattatatct
     1081 ggatctctca cattctattg agaaaactta cgaatcagtg gaacgaaccc aaggagaacg
     1141 aacgaaacca gttgatctgg acaaacaaga atctttctct gttctattga tggggattga
     1201 tactggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg ttgccacagt
     1261 cagtcctgaa gacaaacaga caacgatcgt cagcattcct cgtgatacgt atgtcgacat
     1321 cgtaggtcgt gacacaaaag acaagatcaa ccacgcttac gcattcggcg gagctgccat
     1381 ggcgatggat accgtacaaa aatatgtaga catcccaatc gatcactatg tctctatcaa
     1441 tatgaaaggg ctaaaggaac tcgttgatgc agttggtggt atcgaagtca ataatgacct
     1501 cactttctca caagatggct atgactttcc aatcggtaaa attacattga acggggatca
     1561 ggcattatct tattctcgta tgcgttatga agacccaaat ggtgattacg gtagacaagg
     1621 gcgccaaaga aagatcgtag aaggcgtagc gaaaaaagta ttgagtctaa acggtgtcag
     1681 caactatcaa tccgttttga atgctctaga gtcgaatatg aaaacagaca tgaactttga
     1741 tgaaatgaaa aaaattgctt tagattatcg ggaggcattt ggtacgatta agcaagatca
     1801 gctgcaagga gaaggcttca tgcaagataa tatctcctat caaaaagtca gtgatcaaga
     1861 acttgatcgt gtgaaaaaag aattaaaaga tcaaatgaat ctagagaata aataggaaag
     1921 tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttgaaacat tacgcaaaca
     1981 cttggtaata atcatcatct caatgtttgc tggcttggcc atctcaggaa tcgtaacatt
     2041 tttcatcttg acaccaaaat acagttcaca agcacaattg atcgtgacac tgccacaatc
     2101 agacactgca aatgtcaatg atgtcaatac gaacctgcaa atgatcaata cgtataaaga
     2161 tatgatcgtc agtgatttag tgctcaatga agtcaaagat cgtttagaaa cagaagacaa
     2221 tgtgaaaatg actgctgaac aaatcaaaga tgccatctca gttaatcagg cagaaaattc
     2281 acaaatgttc tcgattcagg caataagtac gaatgcagtg gctgctcaac agatcgctaa
     2341 cacaacttca caagttttcc aagagaacgc aaaagatgtg atgaatgttg ataaaatctc
     2401 gatcatttca ggtgcagttg ccaatacgac acctgtttcg ccaaataata aattgaattt
     2461 aatgactggc ttaatgttag gaatgatggt aggtgtaggt ctggcattct tattggaatt
     2521 actcgatcgg acagtgaaag atgaaaaata tgtgacagat acattaggat tcccaatctt
     2581 aggaacagtt ccagaaatgt cagcgaaaga attgaatgct ggtatccgta aaaaaggacc
     2641 agctcagaca caaccaaaag ctacaaacga taaaacagcg ccatcacgca gaagccgctc
     2701 aagagtctag gaggaaagaa aatggcacga acacagaaac aaaaagcagc ccaaactcat
     2761 gcagtcagtt tggtcacact caccaatccc tcttcaccaa tcgctgaaca atatcgtacg
     2821 attcggacga atatccagtt tgcctccgca gctggtcagc aaatcaaaac gattgttgtg
     2881 acttcatcag gacctggaga agggaaatcg accactgcag caaacattgc cgttgttttt
     2941 gccaaatctg gtcaacgggt gctgctagtc gatgcagact tacgtaagcc cgtcatctat
     3001 aaaacgttca agttgaacaa cgcaagcgga ttaagtactg ttcttagttc atcaggaagt
     3061 gtagcagatg agatccaacg aacaccagtc gacaatttgt cgattctgcc aagtggacct
     3121 aaaccaccaa atccatcaga actactaagt tcgccaagaa tggatcagat tttagcagaa
     3181 gcaagacaac tatttgatgt agtgatcttc gacatgccgc cagtcgttgc cgtaacagat
     3241 gcacagatca tgtcttcaaa aacggatggc accttattgg tcgtacggga aaatacgtca
     3301 cgaaaagaat cattgactaa agcaaaagaa ctgttagata tggtacaagc acgagtctta
     3361 ggtgtcgtat acaacggagc agaacacagc aaagatgctg gttattacta cggaaattaa
     3421 ggaataagaa gagaggagac aagagaaatg atcgatctac attgccatat cctaccagga
     3481 atcgatgacg gggcagaaaa cttagaagca agtattgcaa tggctgaaaa agcgattcag
     3541 caaggcatta cgcatatttt atgcacacca caccacaata atggaaaata cagcaatgaa
     3601 aaatcacaag tcatttctct agtcgcttcg ttacaagccg aattagagaa aagacagttg
     3661 cctttgacgt tattggaagg acaagaagta cgtattaccg gaacgctcat cgaagacatc
     3721 caccgagatg aaattttatt tacagattta gatgatacat atcttctgat cgaatttccg
     3781 acactggaag tgccgttata tgcagaacgg cttttttttg agttgttaga actaggaaag
     3841 ataccagtga tcgtccatcc agaacgcaac gcgcattttc gagaagaccc aaatcatttg
     3901 cttccgttcc tagatatggg atgtttggcc caacttactg cgccaagtta tgttggtgta
     3961 tttggcaaag atatccagaa aacagccaaa acaatggtca aacataatct cgtgcagatg
     4021 gctgcttctg atgcacacgg ggtctctaag cgcacctttt acttaaaaga gtgctatgag
     4081 cagattgcca aggagtttgg taaggaaaaa gtagtacaga tgaaacaagt cgcaaaggat
     4141 ctgatcaacg gggatgagat caattatcca atctatgaag aggtaaaaaa gaagaagttt
     4201 ggtttatttt agttctttat tcgtagataa aaaggaaatg aggagagagt gagagatgta
     4261 tagaaaaaac tcaagtggtt ggtctaaaca tattgatttt attttattag acttattttg
     4321 tgtccagctt gctttttata tatcatatgt aatgcgacag ggtgactgga atccgtatgt
     4381 gattcctatt tatcgccaca tggcaatttt tatagaactt gagaatctta cgattatatt
     4441 tttatttgag gcatataaaa atgttttaaa aagaggatat tataaagagg ctgtagcttc
     4501 tataaagcaa tcttttatgc tgttacttgt gtgcagttta taccttatta caatgcagga
     4561 tggaaatgat tattccagag tttctttatt tataatgggg gtggtgtatg gattattaac
     4621 atatataatt cgtatacttt ggaaagggtt gttgcatcac cgcatgaaaa acggtgaaga
     4681 tgtatcgtta gttattgtaa catctcaaaa gattgcaaaa aatgtagttc gtaatcttaa
     4741 agagaagaat tatggcatgt ataaacttag tggtgtgatt gtagtagaca gagatatgta
     4801 tggaactacc atcggaggtg tcccagtagt tgcaaatgct gataatgcag cggaatatct
     4861 tttacagaat tgggtagatg aagtattcat caatgttgac gaaagtgttc catatcccaa
     4921 agaattaatc gccagatgtt cggaaatggg acttgctgta catatgaatc ttacaaaagt
     4981 gtctgatagt acacgtggaa aacaatttat tggccgagtt ggtgattata ctgttattac
     5041 atctagtatt aatgttatga cgatgaggca agcatttata aagagaacga ttgacattct
     5101 tgctggaatt attggatgta ttgcaacagg tgttattttt atcttcgtag caccagttat
     5161 ttacatcaaa tcaccagggc cgattttctt tgcacaagaa cgtattggtc aaaatggtaa
     5221 gacatttaag atgtataagt tccgtagtat gtatatggat gcagaagaac gcaaagcaga
     5281 gttgatgaaa gaaaataaga tgagtaataa tctcatgttt aagatggatt ttgacccaag
     5341 aatcattggc aataaaatac ttcctgatgg aacaaagaag acaggaattg gacaatttat
     5401 tcgatctaca tcattggatg agtttccaca attttataat gtcctaatgg gaaaaatgag
     5461 cctggtgggg tttagacctt gcttgaagtc agaatatgag aagtatgatt ttcatcatcg
     5521 tgcgagaatt gcgatgaaac ctgggattac tggaatgtgg caggtgagtg gtagaagtga
     5581 tatcacagac ttcgaagagg ttgtaaaact tgatgcagaa tatatcagga attggagtat
     5641 gggattagat tttagaattt tgtttaggac agtcaaaact gtgcttcata gaaacggttc
     5701 catgtaagat aaagatacta tagaaggagt aaaacgtgat ggcaagaaaa aaattaaaaa
     5761 tctgtatgct aggtcataaa agaattccat caagagaagg tggaattgag atagtggtag
     5821 aagaattggc aacgcgaatg gttgcacttg ggcatgaagt gacttgctat aatcgaagtg
     5881 gtcatcatgt tagcggaaag gaatttgacc agcataaaaa taaagaatat aaaggtgtaa
     5941 aattaaagac tatttttaca ttaaatataa aaggaattgc agcaatgagt tcttcagtat
     6001 ttggtggaat tagagcagca ttcggaaaat atgatattgt tcattttcat gcagaaggac
     6061 catgtgctat gttgtggttg ccaaaacttt ttgggaaaag atgtgttgca acaattcatg
     6121 gtctagatca tcaaagagaa aaatggaaca agcttgctag tacatacatc atgttagggg
     6181 aaaagtgtgc tgtaaaattt gcagatgaaa taattgtact tagtgaaagc gtccaaaatt
     6241 attttgaaga tatatatgga agaaaaacaa ggtttatacc aaatggcgtt aaaaaaattg
     6301 aaataaaatc agcaggattg attacagaaa agtatggatt aacaaaagat tcttatattt
     6361 tgtttttggg gcgattggtt cccgaaaaag gaattcggta tctaattgag gcatttaaag
     6421 atgttcagac agataaaaaa cttataatag ccggaggaag ctctgataca gatgaatttg
     6481 caaatgaact aaaggaatta gcaaaaggtg atgagcgaat tatatttact ggatttgtac
     6541 aaggacagga attagaagaa ttatatagta atgcgtatat ctatacactt ccaagtgatt
     6601 tagaaggaat gccattaagt ttgttagaag caatgagtta tgggaactgt tgtatagttt
     6661 caaatatctc agagtgtacg gaagtagtag aagacaaggc tatgattttt aagaaatcgg
     6721 atgtaagtga tttaaagata agattagaag aggcatgcaa tcaatcggaa atggttaaag
     6781 ttttgaagaa tcaagctact gagtttattt gtagtaaata taattgggat aaaatcgtac
     6841 aagaaacatt aaatttgtat aggggaaata tataatgatt cgaatattac agtgtgtgaa
     6901 tgatatgcat cgtgcagggt tggagacgat gctaatgaac tattatagga atatagatcg
     6961 aactaaaatt cagtttgatt ttttgacaca tcgtccatat aaaagtgatt atgatgagga
     7021 aataataagt cttggaggaa aggtctatta tgcaccgaga ctttatccac aaaattatcc
     7081 taaatatttt aaatggatgg aacaattttt taaggatcat ccagaatatg agattgttca
     7141 ttctcatatt gatgcgatga gttatttacc tttaaaagct gcaaaaaaag ctgggattcc
     7201 gatacggata gcacatagcc ataatacatc gatagataga gattttaaat atcttttaaa
     7261 gcaatattat cgatgtaagt taacgtctgt tttgacacat gaattttcat gtggaaaagt
     7321 agcaggtgat tttttgtttc gaaatgataa attcactttg attccaaatg cagtggatgc
     7381 taaaaaattt tattatgatg aaagtattag aaatgatgta cgaatgaaat taggaatttc
     7441 aagtcagacg tttgtaatgg gacatattgg aagaatttca tattctaaaa atcatcgttt
     7501 tttgattgaa atttttaaag aatttcacgc tattaagaat aattccgtat tattaattat
     7561 tggaactggt gatatggaag aagaaataaa aaattatgca aagaaaagtg gcatagatga
     7621 tgatattaaa tttttaggaa atagaaatga tatagaaaaa ttttatcaag catttgacgt
     7681 attaatgttg ccatcattat ttgaaggggt tccgttagtg ggtattgaag ctcaatttgc
     7741 agatttaccg tgtttctttt cggagaaagt tcctacagaa gttgctttca gtgataagac
     7801 caatttcata agtttaaatc aatcggcaaa agaatgggcg agagagattg caaatgtaga
     7861 tatctctcat cgagattcag aacgttcgat tttaataaag gcagattata atattcaaac
     7921 agcatacaaa atcttagaat caaaatatta tgagttattt gaattaatac aaagaggtta
     7981 aagaatatga cacagaaaca ttcaagtata aaaagaatta tacatcgcat atccgaagaa
     8041 ttgaatgcaa caagacgaat tgcaaagata acatcttgga aggaagcatt tgccacgtta
     8101 caagctaaaa ttgatattca agtcatgaat cgtaatggat ataatgaaag taaaaaacag
     8161 aaggaacatt tattaaaaaa acatgaggta atgctgaagt attatgaaaa gactttttca
     8221 gattttttga aaacatatga ttatagtcaa aatcaaataa ttaatcaatc aaaatcacca
     8281 tattctaact gcatttgggt ttgttggtgg cagggattgg atcaatcgcc ggaaattgta
     8341 aaagcttgtg taaaatcaat aaaacgaaat gcagggaaac atactgttat aattttaaca
     8401 gaagataatt atagacaata tgttgatata ccaaaatggg tggaagaaaa gaaaaacaaa
     8461 ggaatcatta cgagaacaaa ttattctgat ttattaagat tatctttgct agcaaaacat
     8521 ggaggtttgt ggttagacgc cacattttat tgtacaaaac caataccaga tgagtatttt
     8581 gatatcgata tttggtctat taggaggcct gattatgctc acgcatccgt agcatcagga
     8641 tattttgcgg gttattcatt agcatgtaat caagagtcta gatggatgtt tgctataatt
     8701 agagactttt ttttaaatta ttggaaaaat aatgatatga tggtggatta tttgatgata
     8761 gattatatga ttgttttagc acagagaata aatcatgaaa tagcacagac atttgataaa
     8821 atacctagta acaatccatt atgtgacgaa ttgattaaaa atttaaataa tatatatgat
     8881 gaaaaagtat ggaacttatt aaagaaagat actttactgt ttaagttgac atggaaacaa
     8941 tcttttaaaa agcaaaaaga tgataaagtt actttttatg ggatgttggt agaaaacaaa
     9001 ttattataaa tattagaggt ataaaaagtt atgaaacaca aggtaagtgt gatagtacca
     9061 atttacaatg ttgaaaaata tttaagaaaa tgtgtggata gtttattgag tcagacatta
     9121 aaagagatag aaatcgtatt agttgatgat ggttcgccag atgcttcggg ggaaatagca
     9181 gatgagtatc aaaaaaaata ttctaatgta aaaacaatac atagagaaaa tggggggtta
     9241 ggaccggcaa gaaatacagg aattgagaat gcgacaggag aatatgtggc ttttcttgat
     9301 tcagatgatt gggtagaaag tgatatgtat gaaaaattat acttagttgc gtccaaagaa
     9361 aatgctgata tagtagttag tggacattgt gattttgcga atgacaaagc tatggtgaaa
     9421 aagcaacatc ctttgagtgg gaatgtatat gatacgccta atagtattaa aaatgtacga
     9481 aaaaatttat ttggacatag tccaaaagat caagaagtag aggccttccc gatgtcagta
     9541 tgtatgtctt tgtataataa aaatctgcta gataaatata gactaagatt tgaaaaaatt
     9601 ttatctgaag atacaatttt taacttgaat gcatatgatg tcgcaaataa aattgctttt
     9661 acagaatata cagattattg ttatagaaaa gaagaacaag agtctattac aaaaagttta
     9721 tctccaaata agcagacgga atttaaagaa tttttaataa ctctaaaagc tatagcatcg
     9781 aaagaagatt tagaatgtca aatcagagca aaacgtatgg caattgatta ttgtaggctt
     9841 tatgtgggct tagttgatga tgcaaatttg ggaattaaag agaaaagaaa gtatataaaa
     9901 aaatttattg aagatgaggg attaattcaa tgttggaagg gatatcctac aaatacgcta
     9961 ccatttcaac aaaaattttt tcataaaatg cttgaatgta agtgttatac aatattgttg
    10021 ttgttaagta gaattagaaa gataattaaa aatatgacca gaaaaaaatt gtttataata
    10081 ttataaacta aaagtatatc aaataataga aattaacaag ttacaaaaaa a
//
LOCUS       DAZUKT010000096        10083 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000096 DAZUKT010000000
VERSION     DAZUKT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.098"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..2229)
                     /locus_tag="ACG816_002515"
     CDS             complement(109..2229)
                     /locus_tag="ACG816_002515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4921356.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="ACG816_002516"
     CDS             2459..3637
                     /locus_tag="ACG816_002516"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4921357.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="ACG816_002517"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="ACG816_002517"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4921358.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="ACG816_002518"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="ACG816_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4921359.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="ACG816_002519"
     CDS             complement(4990..6243)
                     /locus_tag="ACG816_002519"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4921360.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="ACG816_002520"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="ACG816_002520"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4921361.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="ACG816_002521"
     CDS             7821..9167
                     /locus_tag="ACG816_002521"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4921362.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="ACG816_002522"
     CDS             complement(9475..9861)
                     /locus_tag="ACG816_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4921363.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10083)
                     /locus_tag="ACG816_002523"
     CDS             complement(9910..>10083)
                     /locus_tag="ACG816_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS4921364.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3214 a         1839 c         2054 g         2976 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgt
//
LOCUS       DAZUKT010000097         9866 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000097 DAZUKT010000000
VERSION     DAZUKT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.100"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACG816_002524"
     CDS             complement(18..263)
                     /locus_tag="ACG816_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4921365.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(377..955)
                     /locus_tag="ACG816_002525"
     CDS             complement(377..955)
                     /locus_tag="ACG816_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4921366.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1032..1562)
                     /locus_tag="ACG816_002526"
     CDS             complement(1032..1562)
                     /locus_tag="ACG816_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4921367.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="ACG816_002527"
     CDS             complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="ACG816_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4921368.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2419..2811)
                     /locus_tag="ACG816_002528"
     CDS             complement(2419..2811)
                     /locus_tag="ACG816_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4921369.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEALM
                     GYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2808..4220)
                     /gene="cysS"
                     /locus_tag="ACG816_002529"
     CDS             complement(2808..4220)
                     /gene="cysS"
                     /locus_tag="ACG816_002529"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4921370.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4217..4753)
                     /gene="epsC"
                     /locus_tag="ACG816_002530"
     CDS             complement(4217..4753)
                     /gene="epsC"
                     /locus_tag="ACG816_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4921371.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4839..5078)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5075..6535)
                     /gene="gltX"
                     /locus_tag="ACG816_002531"
     CDS             complement(5075..6535)
                     /gene="gltX"
                     /locus_tag="ACG816_002531"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4921372.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6705..7862)
                     /locus_tag="ACG816_002532"
     CDS             complement(6705..7862)
                     /locus_tag="ACG816_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4921373.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7896..9266)
                     /gene="radA"
                     /locus_tag="ACG816_002533"
     CDS             complement(7896..9266)
                     /gene="radA"
                     /locus_tag="ACG816_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4921374.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9329..9856)
                     /locus_tag="ACG816_002534"
     CDS             complement(9329..9856)
                     /locus_tag="ACG816_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4921375.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2506 a         2212 c         1784 g         3364 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa gaaatgcaat aaaaataaat aagaagctca
      361 ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt
      421 ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt
      481 ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact
      541 ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaatcgct aaaggttctt
      601 gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga
      661 cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt
      721 gattgcttat aatgctttta taataacttc caaagctaac acctttgctt ttttcgtaag
      781 ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat
      841 ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg
      901 cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat
      961 aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt
     1021 agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt
     1081 aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt
     1141 tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt
     1201 cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc
     1261 acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt
     1321 tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc
     1381 ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt
     1441 gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg
     1501 acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc
     1561 atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca
     1621 ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct
     1681 tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct
     1741 ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct
     1801 gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt
     1861 tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt
     1921 ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg
     1981 tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt
     2041 cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct
     2101 tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc
     2161 acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct
     2221 tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca
     2281 aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca
     2341 gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga
     2401 tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat
     2461 caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa
     2521 ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc
     2581 atttcttcct ctgcgataat aaagttcttc ttctgtcaaa agatgttctt ccagcatttg
     2641 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     2701 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     2761 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     2821 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     2881 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     2941 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     3001 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     3061 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     3121 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     3181 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     3241 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     3301 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     3361 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     3421 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     3481 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     3541 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     3601 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     3661 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     3721 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     3781 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     3841 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     3901 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     3961 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     4021 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     4081 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     4141 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     4201 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     4261 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     4321 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     4381 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     4441 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     4501 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     4561 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     4621 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     4681 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     4741 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     4801 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     4861 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     4921 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     4981 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     5041 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     5101 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     5161 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     5221 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     5281 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     5341 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     5401 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     5461 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     5521 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     5581 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     5641 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     5701 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     5761 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     5821 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     5881 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     5941 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
     6001 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
     6061 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
     6121 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
     6181 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
     6241 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
     6301 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
     6361 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
     6421 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
     6481 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
     6541 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
     6601 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
     6661 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
     6721 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
     6781 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
     6841 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
     6901 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
     6961 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
     7021 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
     7081 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
     7141 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
     7201 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
     7261 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
     7321 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
     7381 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
     7441 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
     7501 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
     7561 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
     7621 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
     7681 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
     7741 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
     7801 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
     7861 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
     7921 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
     7981 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
     8041 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
     8101 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
     8161 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
     8221 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
     8281 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
     8341 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
     8401 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
     8461 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
     8521 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
     8581 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
     8641 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
     8701 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
     8761 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
     8821 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
     8881 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
     8941 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
     9001 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
     9061 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
     9121 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
     9181 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
     9241 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
     9301 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
     9361 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
     9421 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
     9481 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
     9541 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
     9601 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
     9661 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
     9721 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
     9781 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
     9841 atcctctttt tctcaccggt tttcct
//
LOCUS       DAZUKT010000098         9680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000098 DAZUKT010000000
VERSION     DAZUKT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.101"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG816_002535"
     CDS             33..656
                     /locus_tag="ACG816_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921376.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG816_002536"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG816_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4921377.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG816_002537"
     CDS             2152..3033
                     /locus_tag="ACG816_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921378.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG816_002538"
     CDS             3036..3806
                     /locus_tag="ACG816_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921379.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG816_002539"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG816_002539"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4921380.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG816_002540"
     CDS             5117..6280
                     /locus_tag="ACG816_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4921381.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG816_002541"
     CDS             6397..6807
                     /locus_tag="ACG816_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4921382.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG816_002542"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG816_002542"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4921383.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG816_002543"
     CDS             9380..9664
                     /locus_tag="ACG816_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921384.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3361 a         1640 c         2083 g         2596 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt
//
LOCUS       DAZUKT010000099         9665 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000099 DAZUKT010000000
VERSION     DAZUKT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.102"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACG816_002544"
     CDS             complement(<1..632)
                     /locus_tag="ACG816_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4921385.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACG816_002545"
     CDS             975..1391
                     /locus_tag="ACG816_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4921386.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACG816_002546"
     CDS             1388..1858
                     /locus_tag="ACG816_002546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4921387.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACG816_002547"
     CDS             1872..2645
                     /locus_tag="ACG816_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4921388.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACG816_002548"
     CDS             2659..3471
                     /locus_tag="ACG816_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4921389.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACG816_002549"
     CDS             3525..4490
                     /locus_tag="ACG816_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4921390.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACG816_002550"
     CDS             4606..7329
                     /locus_tag="ACG816_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4921391.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACG816_002551"
     CDS             7342..7974
                     /locus_tag="ACG816_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4921392.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="ACG816_002552"
     CDS             8873..>9665
                     /locus_tag="ACG816_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921393.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1477 c         1884 g         3044 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DAZUKT010000100         9432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000100 DAZUKT010000000
VERSION     DAZUKT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.103"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACG816_002553"
     CDS             <1..680
                     /locus_tag="ACG816_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921394.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACG816_002554"
     CDS             800..1000
                     /locus_tag="ACG816_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4921395.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACG816_002555"
     CDS             1076..1273
                     /locus_tag="ACG816_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921396.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACG816_002556"
     CDS             complement(1570..3117)
                     /locus_tag="ACG816_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS4921397.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACG816_002557"
     CDS             complement(3201..4004)
                     /locus_tag="ACG816_002557"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4921398.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG816_002558"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG816_002558"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS4921399.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG816_002559"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG816_002559"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS4921400.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG816_002560"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG816_002560"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS4921401.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACG816_002561"
     CDS             complement(6389..7318)
                     /locus_tag="ACG816_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4921402.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACG816_002562"
     CDS             7513..8196
                     /locus_tag="ACG816_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFS4921403.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACG816_002563"
     CDS             8357..9400
                     /locus_tag="ACG816_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4921404.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAZUKT010000101         9118 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000101 DAZUKT010000000
VERSION     DAZUKT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.104"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            482..1807
                     /locus_tag="ACG816_002564"
     CDS             482..1807
                     /locus_tag="ACG816_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4921405.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            1800..2147
                     /locus_tag="ACG816_002565"
     CDS             1800..2147
                     /locus_tag="ACG816_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921406.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            2144..2326
                     /locus_tag="ACG816_002566"
     CDS             2144..2326
                     /locus_tag="ACG816_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921407.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            2462..3319
                     /locus_tag="ACG816_002567"
     CDS             2462..3319
                     /locus_tag="ACG816_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4921408.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            3339..3899
                     /locus_tag="ACG816_002568"
     CDS             3339..3899
                     /locus_tag="ACG816_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFS4921409.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            4865..5632
                     /locus_tag="ACG816_002569"
     CDS             4865..5632
                     /locus_tag="ACG816_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFS4921410.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            5658..6203
                     /locus_tag="ACG816_002570"
     CDS             5658..6203
                     /locus_tag="ACG816_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921411.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            6204..6770
                     /locus_tag="ACG816_002571"
     CDS             6204..6770
                     /locus_tag="ACG816_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921412.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            6785..7033
                     /locus_tag="ACG816_002572"
     CDS             6785..7033
                     /locus_tag="ACG816_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921413.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            7052..7291
                     /locus_tag="ACG816_002573"
     CDS             7052..7291
                     /locus_tag="ACG816_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921414.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            7308..7892
                     /locus_tag="ACG816_002574"
     CDS             7308..7892
                     /locus_tag="ACG816_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921415.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            7999..8280
                     /locus_tag="ACG816_002575"
     CDS             7999..8280
                     /locus_tag="ACG816_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4921416.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDKPVDVLDTDEKLEEWFNEA"
     gene            8270..8611
                     /locus_tag="ACG816_002576"
     CDS             8270..8611
                     /locus_tag="ACG816_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4921417.1"
                     /translation="MKPNEIVTVYAAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         3428 a         1216 c         1841 g         2633 t
ORIGIN      
        1 ctaatcgaag taaaacccaa agccatatga acccctgaat cagcgaaagt ttatgatgat
       61 ttggattact aatagtttag gaattagatg gtaaatctaa attaaacaat cgttgatttg
      121 gcaacgaaat agtgagggaa aatccactca ctgaagggta aactccgtca agttatgtga
      181 ccagacggaa ggacgaatgt tgttgaggtt ttagaaccat ataaaacagc atagttctca
      241 aattgtttaa aatgattgtc aagttaaaaa aatctgggat catataaaat agtatagttc
      301 tcaaatatga ttagaaaccc tattaaagag caatattaag ccaacttact taaatcgttt
      361 aattaagaac aataatctct tttagtcaga cttttagtct gacttttttg tatttccaaa
      421 atttattctt tacaaaaacg aacatacgtt tgtataattg cttgtgaatg ggggaataaa
      481 aatgtttgat tacagtagag aaccggtgaa tgactatttt ttaatcgaca tgaagtcttt
      541 ctatgctagc gtggaatgta ttgaacgaaa tttagaccca atgactacac cgttaattgt
      601 catgagtaga tcggacaaca ctggttcagg tctgactcta gcttcttcac ctactgcaaa
      661 gaaaaaatat catattacta atgttagtag agctactgat ttaccttatc cattaccaaa
      721 agaacttcat attgttccac cacgaatgaa cttatatata aaaaaaaata tggaagttaa
      781 tcagattttt agaaagtatg tcgcagatga agatttattg atttattcga ttgatgaatc
      841 aattttaaaa gtgacaaaat ccttaaattt atttactagt tcaggaacaa gaagcgagag
      901 aagaagagaa ttagctaaaa taatcaaaga tgagattcag aaagaaacag gtctaatagc
      961 aacagtcgga ataggagata acccattatt agctaaactt gcactggaca atgaagcgaa
     1021 gagtagtaaa gaatttatag ctgagtggac ttatgagact gtacctgaaa aaatatggaa
     1081 aataaacgag atgactgact tttggggcat tggatcacgc atgaaaaaaa gattgaatca
     1141 tatgggaatt atgagtatag aagatttagc aaattggaat ccctacacaa ttaaaagtca
     1201 gttaggtgta atcggactcc agttatattt ccataccaat ggaattgatc gtacggatat
     1261 tgctattcca ccagaaccaa ccaaagaaaa gtcttatggg aattcacaag ttctaccaaa
     1321 agattatacg aggagagaag aagtagaact agtagtcaag gagatggcag agcaagtagc
     1381 cattcgaatt agacaacaca attgcaaaac tggttgtgta cgcttagcaa ttggtacgtc
     1441 aattttggaa gaaaaatcag gtttctcgca ccagatgaaa ataccaatta cagataatac
     1501 gaaagaatta caaaattatt gtcttttttt attcaataag ttttatgagg ggcaggaagt
     1561 acgacatata ggagtaactt atagcagatt agtatacaca gattccctac aattagattt
     1621 atttagtgat ccaagaaaaa agattgctga agaaaatttg gataggatta ttgataagat
     1681 tagacaaaaa tatggattta cagctattat acatgcttca tctatgttag aaggtgcaag
     1741 aagtattacg agaagcactt taataggagg acacgcagga ggattaggag gtttgcataa
     1801 tgattgaata taacgataga ggacgtttaa aatggtcggg attttatttg tctgatcata
     1861 ctgaaagaat caatgcagac aatgtacaac gtgctaatca taatttggca aaagaacaaa
     1921 tgacgactga agagattgcg aaaatactga gaactgctgt cataaaaaat agacccgtct
     1981 gcattcaaaa agaggaacga aacgctgaag gttactatcc agatgatgta gtaggaaaaa
     2041 ttattggata tgacgagctt ggtgtatttg tggaagaaga aaaagttcac tatgatgaaa
     2101 ttaggaatgt ttctttctcg gaattattaa aatggagtca gatatgaaca ttattaaaaa
     2161 atacgaattc ggttatatta cgtatgaaca gctaatagag gaattatggg gatatggtca
     2221 acaattaatt aatgaagtgg ggattgattg cttttgtttc tatattgaat ccggagcagg
     2281 ataccataag aacagatact atatatttcc atatccacca aaatagtctc ttagatattt
     2341 ttaaattttc tccaaatact ttcaattatt gtgaaatatt tccctttaat taatcaaatt
     2401 ttttataaaa gtaatatata aaataaattg cttgaagtat tatatttaga tatcataatt
     2461 tatggaagaa aaattaatta aaaaggatga gattataatg gggttactgg ttccgagagc
     2521 taatacttat actatacctt taacgaaacg ccaattaatt gctaaaagat atcaaaggat
     2581 tactagagcc atcaatagag agttttggaa ttctgaaagt gatactgctc acagtttata
     2641 cgtaggttct tatgggagag gaacggcaat tagtactagt gatatagata taatagttga
     2701 attaccgatg gcagaatttg acagattcaa aaattattta tcaaatggcc cgtctaagtt
     2761 attacaagtt ataaaaaacg catttcagga aatactacca aatagtgata ttagagctga
     2821 tggacaagtt gttaaaataa atttccatga tggaatcaaa tttgaaatag ttccagcttt
     2881 taatgaaaaa gattattggg gtgagtcaaa agggtttatt tatccagatt caaacatggg
     2941 agggaactgg aaagctacaa atcccaaaaa agagcaagaa gccatgaaat taaagaatac
     3001 aaaaagtaat aatttattat atgctacgtg taaacatttt cgacatgtac gagatacaga
     3061 atttacgagt tatcatcttt caggaatagt aattgacagt ttcgtatatg aggctatggg
     3121 aaattggaaa ttcgttgaaa ataacagtgg gggtcaaaac attagttctg tatcttatga
     3181 gacagcttta ttagaatact ataactcaca taaagttatg ggcggtttaa atttatactc
     3241 tcctggaagc aatcaatttg ttaattctga tagtagtatc atatgtttag aaaaagtatt
     3301 aaaaaaaata gctttataaa gttttatgga ggaacaacat gggagacgaa aaaaagcgct
     3361 tggaagttca attacgtgaa gcatatggac gagtaacgta tacttacacc actcatttaa
     3421 agcaagccga taatattatt tctgacaata aaattttaag gtgtcttcaa attggtttgt
     3481 cagcccttac tactggaggg gtacttagta ccattattac tgataatagt gagttaacct
     3541 ttgttgctgg attatgttca acaatttctc ttggattaaa cttatacttt aagaattttg
     3601 atcttgctga agaagctaaa aatcatcaaa ttgttgccga taaattatgg ttgatcagag
     3661 aagaatacgt aagcttacta acagatttaa atcgtttaag tgttgaagat attgtgaaaa
     3721 aaagagatca attaatgaac gaaactcatg aagcatataa aaatgcccct aaaacaaata
     3781 gtaaaagcta tgaatcagct cagaaagcat taaaatctga agaagagcaa ttttttacag
     3841 aagaggaatt ggataagatg ttaccagagc atcttagaaa aaagagttct aaatattgaa
     3901 ctcttttttt atgcttaaaa ttctcttgta atttttctat tcatctatat ttaagaagaa
     3961 atatgatgat agaaacatct cctaaagcca ccgcaagccg ttctaagcat agcttagccc
     4021 gacattgcta gatatgctca tctcaccatg aacgtttcct aaagccaccg caagccgtcc
     4081 tagccattgc ctagtccgac atcgctagat tagtgcatct cgccataaaa gttttctaaa
     4141 gccaccgcaa gccgtcctag ccattgccta gtccgacatc gctagattag tgcatctcgc
     4201 cataaaagtt ttctaaagcc accgcaagcc gttctaagca tagcttagcc cgacattgct
     4261 agatatgctc atctcaccat gaacgtttcc taaagccacc gcaaaccgtc ctagccatcg
     4321 cctagtccga ttttgcttga tcggttccat ttctgtttat ttagtgggtc aacaccgtaa
     4381 tcttaaatag ccatcgccta tttaagatta ctaggtctgc tttgaaggta aaaagtcttt
     4441 cattcttcaa gacttttagg ctttcaagct acgcctgaaa gcattgaaat tcatcgctct
     4501 ttatttggtt ttgccattta taattcctta cgcccattat ctttttcgcc tgtcatacct
     4561 tgtcagaagt gtttagagct tatctcatct ttggattaat acaagtggcg taaatttttt
     4621 tctaaacgca agcgttacga aaataattta cttaaacact tgtattaatc cggcagataa
     4681 gaacgcattc taatcactcc cgacaaggca agacaggcga aaaagataaa taaatgaacg
     4741 aaaggaagaa acataaaatg caaaaccaaa tagcgatgaa gaaaaaacca cctccctcta
     4801 aaacaatcca aacaaaaaag aattttgaaa aaccaaaaat caaaaacaga aagaagcgtt
     4861 caacatgaga gatagaattt tagaagttaa tcaagaaatg gtaaacaagt taacaaatat
     4921 cagtgggaac tatgaaaagt acgatggtac tttacttgat aattatattt ttcacgatac
     4981 acaaaatatc agaattggca gaagtaaacc gagagcgttc attctcatca aagagaaata
     5041 tttgaatgaa tggaatagca cctatgaaat ggttatgact gataactttg aaaaggtatt
     5101 ggatttcaga aatgctttgt tgggttctga tatgggaaat agcagtcttg aatctattga
     5161 agttgaaaac aagaccgaga atgaaggtga aggattgatt aagaatgtcc tgaaaatcga
     5221 acaaaaatat atcagtgaag atagaagttt ttatagagtc acaagctcgg ttggtctagc
     5281 aaactttgct actaacttaa ttggaaacaa tgctagtgaa tctttgctag tgatgtgtct
     5341 gaatacaaag aatgaggtca ttgcttatag cgaagttttc aaaggtggaa ttaatcaatc
     5401 agtagcgata ccaagagaga tttttcaact agctttgcta aacaattcag caaaaatcgc
     5461 agtatcacat aatcatccga gcggatatga atttccaagt gaaaatgaca aaagttttac
     5521 tacgaatttg aaaaacgcag gaaaaacaat tggtattgaa ttgttagacc atatcatcgt
     5581 aacaagtaaa aacacaagct attactcatt tcaagaaaat ggttctttat aaaaattaga
     5641 gaaaagagga aaacaagatg ggcgaaagaa cacaagcgtt aattacagtc aaatatggta
     5701 acagcaaaac gaaagtaagc gtgtcaattc attatcagtg gggatatggt cgattcatgt
     5761 tgatggacgt tttgaatcaa ggaatatatt taatcaataa agcatatgac ttacgagatg
     5821 aatcaagaga agaattagca aaaggaatct ttgatagaag tacaggagtt atcaattttg
     5881 tcacctatga atctggagtg agtgcttatg atgaaacagg gtttggtggg cgctatgaac
     5941 cttatcaaga aacgattgaa gatgtgatga agttttcaga taacaatgat ggctatgctc
     6001 atttagagat ggtgttcgat gaaaatggat ttagtctaga tgataaaacc agattgcgat
     6061 tgtttgattt tgagggtcaa cagataggac taaacaagtt ttgtgaaagc ggaaaagagt
     6121 ttgctacgaa agattttatt aagggataca gattattgct agaaaattat ggaataaacc
     6181 tagaagaaga gagcgtgaaa taagtgtttg aaaaaagaaa agaccagttc cctaatttag
     6241 aagcgaaaag tcaaaaagtg attgatggtg aaatgattga attattccaa aaaagttatg
     6301 gaaccataga aactaccgca agttgcaaat actactttca aggaaagaga aacacgttta
     6361 aaggctttgc cgattctgat gaagaattgg caaagaaaat cattgattgg cttgtcagca
     6421 gtgaacacgt agcaagagaa gataccgtta gaacaacgat tgagggtgaa gtcctaacag
     6481 gtactagatg tgtttgttgt aatcatgaat ttgtagaaga tgaaggttgc taccgaatca
     6541 atgaatatgg tgattttatt tgccagagtt gccacgagat agaatttcca accgaaagaa
     6601 gttggtttga atacatgcta caagattatg agatggaaac agaaaatggt gaagtagttt
     6661 tttgtaccaa agaggaattg agtaagagaa gcgatgatga aattgaagat tggtgtgtgt
     6721 ggttagtagg gacagatgat tgcgttgcat attacacaac agcagaatag gagtgtgaat
     6781 cagcatggaa ggaatggatt ttatagatca tgaagatttg attgattttg gttatacatg
     6841 gaaaggaatg gtaggtatta gcagaagtct tgctaatgcc ttttacgaaa gaaattatgc
     6901 agtctatgtc ctttatgatg acaatacaga aagccttgtt gatgaagagt ataaattgga
     6961 tttagagaat gttttatatg ggatagaaaa agaagattta gcaaaatata ttttttcatg
     7021 gttgggtcaa taaacgaaag agggtagcaa catgacaaaa ttcaaaagta aaggtatgca
     7081 atggtgttct tattattatg ttgggaatga tgaagagctt ttgagaaaag ccaaacaaaa
     7141 aggttatcca gataagatgt taaaacagat tggctattac atgccagaag agagaataga
     7201 aaaagaatct tgtaaggttc tttttgatag tgaacagcta ttagttttgt taactcaaaa
     7261 taaattgatt tgtcaatttg aaaataatta atatggggtg aagaaccatg aatgagaata
     7321 agatggtatt aagtgaatac gcaagattga gaaccattaa ttggttgaat ggtatgcaaa
     7381 gtagtggcgc ttttgatgga tttgatgagg agaatatgga ctttgaaagt ttatcgaatg
     7441 aggatattag atattatttt atcgaatggt attttacaga accagatgaa gaggtggaag
     7501 atgaaccaga attaatttta gaacgtcaaa gaattttatt cagtgaacaa gatagtaaga
     7561 ggttgtatgg tttatatgac ttacaactga aaggattaaa agcgcagtat gttgctgata
     7621 gttacgatga tatggtagaa cagggggcag atagggttgt cacttattca gaagatgatg
     7681 aagaggaatg ggaaaactat tcagatgatg agatatgctt taatgatggg tatatgccta
     7741 ttgaaatcac cgaagaagaa tatgataaag tgtcggatag cgatgagatt ggtatgtgta
     7801 cggttgtaag tggattggat atagatgtcc aaagagtaga ggttgcatac cagaagatga
     7861 aagaacgtat aaaaagtaaa gtagaatctt gatttgattg gattgtttgc aaagtagcta
     7921 attaagagta taatataatc ataaatgtag ttaatggata taataatgag tatattatac
     7981 tcaaaagagg tgaacgaaat gaaggtaaaa aatccaaaag aaaaaagcag aattcaagtc
     8041 ggaatagaca aggatttaaa agaaaacgca gaaatgatat tggaagagct agggttaaat
     8101 ccaacaacag caatcacaat cctttataag caagtggtcg ctagaggtga gtttccagta
     8161 gagataaagc taagcgaaga ggaaaagcaa ggaatcagat tgcaacagct aactatggat
     8221 aagccagttg atgttctaga tacagatgaa aagttagaag agtggttcaa tgaagcctaa
     8281 tgagatagta acggtctatg ctgcatttac agataaaaat ggtggtaaaa gaagacctat
     8341 tttggtggtt agtgataaag aagatagagt agaatttttt ggaatcacaa gtcaatatga
     8401 gaataaatca gaccaaataa agagagtgta ttttcctatt gcagaatggg aaaaagtagg
     8461 tctaaagaag caatcatgga ttgacgtagg gtctttaaag gctattccca aaagccgaga
     8521 gaacatatca tttaaaaagg ttggtatgct atcagctatt gatacgaaaa agctaaatag
     8581 gtttatcgag agattacaaa gaaaagtcta acaaggcttt tctttttttg tttatatgta
     8641 tattaagata tacgtttata taaacgtatt tatgtatatt aaaatacacg taaatatata
     8701 cgtgattata aaaattaaga aaatattaag tattgttaaa tagtgataat tagtaatata
     8761 cgaattataa gatacatttt tatatttaat gtatgtaaat aaataaaatg actgacggat
     8821 attgaatagt aataaataac aataattcag acggatacag tgagtgaagt agcttgctac
     8881 gaacgaacag gcagaatgaa acggtagttt cattagagca agtcgcttgc gacgtagcgt
     8941 taccactgat gtaactggca agttacatag agagagtttg cttgcaaacg aacgttaccc
     9001 acttgaatag aaaacaaaaa taacatatta gtgaattttg aataggagcc acaactcaac
     9061 caaccgcttg cggtgggtga gtcccacgga ggaaaagacg aacgaacggt cgcgtagc
//
LOCUS       DAZUKT010000102         8876 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000102 DAZUKT010000000
VERSION     DAZUKT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.105"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1094
                     /locus_tag="ACG816_002577"
     CDS             33..1094
                     /locus_tag="ACG816_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4921418.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="ACG816_002578"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="ACG816_002578"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4921419.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="ACG816_002579"
     CDS             2007..3989
                     /locus_tag="ACG816_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4921420.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACG816_002580"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACG816_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4921421.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4711..6135
                     /locus_tag="ACG816_002581"
     CDS             4711..6135
                     /locus_tag="ACG816_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4921422.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6316..7089)
                     /locus_tag="ACG816_002582"
     CDS             complement(6316..7089)
                     /locus_tag="ACG816_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4921423.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7086..7991)
                     /locus_tag="ACG816_002583"
     CDS             complement(7086..7991)
                     /locus_tag="ACG816_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921424.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8145..8465)
                     /locus_tag="ACG816_002584"
     CDS             complement(8145..8465)
                     /locus_tag="ACG816_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4921425.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2804 a         1588 c         1883 g         2601 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata
     4621 tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat
     4681 aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt
     4741 ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt
     4801 attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta
     4861 ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat
     4921 ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc
     4981 agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg
     5041 atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc
     5101 gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat
     5161 gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt
     5221 gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt
     5281 atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg
     5341 gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa
     5401 aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac
     5461 gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag
     5521 gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg
     5581 atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg
     5641 attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc
     5701 aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt
     5761 ttactttcat tattattcac tgacagtgct tataactttg cttacacact tggtacagca
     5821 gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg
     5881 aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg
     5941 ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat
     6001 ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag
     6061 attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg
     6121 atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat
     6181 tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa
     6241 taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc
     6301 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
     6361 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
     6421 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
     6481 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
     6541 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
     6601 catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa
     6661 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
     6721 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
     6781 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
     6841 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
     6901 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
     6961 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
     7021 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
     7081 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
     7141 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
     7201 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
     7261 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     7321 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     7381 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     7441 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     7501 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     7561 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
     7621 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
     7681 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
     7741 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
     7801 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
     7861 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
     7921 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
     7981 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
     8041 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
     8101 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
     8161 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
     8221 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
     8281 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
     8341 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
     8401 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
     8461 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
     8521 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
     8581 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
     8641 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
     8701 gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct tatttctata
     8761 tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat aatttttgtg
     8821 ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa acaact
//
LOCUS       DAZUKT010000103         8775 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000103 DAZUKT010000000
VERSION     DAZUKT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.106"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1142
                     /locus_tag="ACG816_002585"
     CDS             135..1142
                     /locus_tag="ACG816_002585"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS4921426.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1154..2158
                     /gene="neuB"
                     /locus_tag="ACG816_002586"
     CDS             1154..2158
                     /gene="neuB"
                     /locus_tag="ACG816_002586"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFS4921427.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2155..3303
                     /gene="neuC"
                     /locus_tag="ACG816_002587"
     CDS             2155..3303
                     /gene="neuC"
                     /locus_tag="ACG816_002587"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4921428.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3276..3953
                     /locus_tag="ACG816_002588"
     CDS             3276..3953
                     /locus_tag="ACG816_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4921429.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3904..5085
                     /locus_tag="ACG816_002589"
     CDS             3904..5085
                     /locus_tag="ACG816_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4921430.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5098..6075
                     /locus_tag="ACG816_002590"
     CDS             5098..6075
                     /locus_tag="ACG816_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4921431.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6075..6983
                     /locus_tag="ACG816_002591"
     CDS             6075..6983
                     /locus_tag="ACG816_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFS4921432.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            6984..7736
                     /locus_tag="ACG816_002592"
     CDS             6984..7736
                     /locus_tag="ACG816_002592"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4921433.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            7741..8688
                     /locus_tag="ACG816_002593"
     CDS             7741..8688
                     /locus_tag="ACG816_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4921434.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3226 a         1108 c         1677 g         2764 t
ORIGIN      
        1 tacttcatat tttaatcaat caaatgttga ttcgttacgt tttgacatca ttattgataa
       61 ttagtttagt tatatatttt ttaaagatta agaatcgaat tatagatgac tataattcat
      121 aggaggagat ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca
      181 ttggaagtga aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata
      241 gtagaggtga agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga
      301 gatttttaat tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg
      361 atattatttt tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg
      421 aagctgtaaa aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata
      481 atgtaaaaca ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg
      541 gtgcgacaaa gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa
      601 cagaaacaat attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta
      661 ttcctctttt tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata
      721 tgacacgatt tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga
      781 attcaaaatt tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag
      841 ctcaatcatt aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa
      901 aagttgtagg tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa
      961 ttgaacgctc atatgaaaat gataaaatgt atatcattcc accacactat catgctactg
     1021 acgaactgta caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag
     1081 aattaatgag tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt
     1141 aaggagtttt atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa
     1201 tggaagtcta gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc
     1261 ggttaaattt caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa
     1321 atatcagaag gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga
     1381 attatctcaa tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt
     1441 tttgtctact ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg
     1501 atttaaaatt ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta
     1561 tgggttgcca gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt
     1621 agattgtttc atagatagtc cagttactat tttacattgt acttcaaatt atccggctag
     1681 ttctgaagat attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt
     1741 tgggtactcc gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc
     1801 taaggtaatt gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc
     1861 ttctttagat cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt
     1921 tttaggtgat ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag
     1981 aaaaagccta gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct
     2041 tacaataaaa cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa
     2101 aaaagttaaa agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa
     2161 aaaatactta tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag
     2221 aatttactac aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat
     2281 aaatatggat ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt
     2341 caaagtttgt ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata
     2401 aatggtttca caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat
     2461 agaggagaaa tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat
     2521 attcacggag gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa
     2581 atagcatctg ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg
     2641 gaagattctt ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat
     2701 agattaccta gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa
     2761 tacgttttgc aagtatttca tccagttgta accgaactag aaaatataga aaaacaaatt
     2821 ttagctttga tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat tttacctaat
     2881 agtgatgctg gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa aacggatatt
     2941 atttatatta cgaatttaga gccggaggaa tacctgacta tattaaaaga gtgcaaattt
     3001 atgatcggaa attcttcttc tgggataatt gaagctgcat cattcaaaat accagtaata
     3061 aatttaggaa ccagacaaaa tggacgagaa caatctaaaa atattataaa tgcaaatcta
     3121 aatgaaaatg aaattgaaac tgctttgaat aaaatacaag atcctcagtt tataaaaagc
     3181 ttgaaaaatg ttaagaacat ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca
     3241 ttgaatcaaa tattgaatga tggggaagat attaagtgga ttcaaaaaca aatagcttat
     3301 taaaatatac tagtgatcca tacattatta ttggggctgg cggacacgca aaagtcatta
     3361 ttgaaacctt aagattagaa aataagttaa tatatggatt ttttgatgat aagcaaaata
     3421 aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt gaatagctgg gcggatagca
     3481 aaaaaaaaat ctataaattt attgtagcta ttggtgactg tgtagtaaga aaaaagatta
     3541 taaaaaaatt gaatatgcca agtgagcaat atggaatagc tgttcatcca agtgctatag
     3601 tggcaaaaga aatttcaatt ggttatggaa ctgttatttt tgcgaatgct gtaattaacc
     3661 cagatgctgt aattggagag catgctataa taaacacagg tagcattgtt gagcatgatt
     3721 gtagaattaa taactatgtt catttatctc caggtgtatg cttagcgggt ggagtacatg
     3781 taggtgtagg aactcaaata ggaattggtt ctcaatgtat ccagatgaag aaaattggat
     3841 cttggtgtat gataggcgca gggagcacta ttgttaagga tattccctca cattccttag
     3901 cttatggcaa tccagctaaa attaagaaag aaggaattaa ttttgaattc tagaatttta
     3961 ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat
     4021 aattggatag cgccattagg tgaaaatgtg gataaatttg aagaaagtat tacaaaatat
     4081 actggttcta agggcggagc ttctgcgtta agttcaggta cagctgccat acatcttgcc
     4141 ttaagactgc ttaatgttgg aaaaaacgat attgtctttg ctccttcatt tacttttata
     4201 gctacagtaa atcctatttt atatcaaggg gctgtaccag tgttcattga ctcagaacct
     4261 aatacatgga atatgagccc tgttgcacta gagagcgcat tagaagaagc tgaaagaaac
     4321 aaccaattac ctaaggcaat catcgtagtg aatttatatg gtcaaatggc tgattatgaa
     4381 cgaattttag ctattgcaaa ccaatttaat gttccagtga tagaagatgc agcggaatcg
     4441 ttaggttcta cttataaggg aatttcgtca ggcttatttg ggacaattgg gatctattca
     4501 tttaacggta ataagatcat cacaacctca ggtggaggag ctctagtgtc tagtgataaa
     4561 aaatatgttg agcattcaag atttttagca acacaagcaa gagatcaagc accgtattat
     4621 caacatagcg aaataggtta taattaccgt ctaagcaata tttctgccgg tatcggacgt
     4681 ggacaaatgg aagtattgaa tgatagagta gctaaacgaa gagaaatttt caatcgatat
     4741 aaggaagctt tttctggaat tgatagaatt gaaatgatgc cagaagtgga tggcatggtg
     4801 ccaaatcgtt ggttgtctac tttaacgatc aatgattata attctattga tcaagtagtt
     4861 aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg gaaaccacta
     4921 catttgcaac cagtatttca aggggtaaaa atgtattacc atgataataa tcttagtgtg
     4981 tcagaaaagt tatttaatag cggcttatgc ttaccttctg gaagtaatat gacagatagt
     5041 gaacaagaat ttgttattga tgagtttaaa aaaatattca agtaggtgtg atattttatg
     5101 aacataggaa ttattggttt aggttccatg gggaaaagaa gacttagact cattcaagaa
     5161 cactttaatg atattttact atgtggtgtt gatactaatg aggcgagacg aaaggaagtt
     5221 gaaaaggaat ttggaatacc aacttataat agtctaacag aggcaaaaaa tttgtttgaa
     5281 ataaattcag tttttgtttg tacatcccct ataacccatg aatttattat aaaagaggct
     5341 ttaaatttag attgtaatat ctttacggaa atcaatttat taaatgaata ttatgatgaa
     5401 ataatcgaag tagctaaaaa gaaagataga ttactttatc tatcttctac atttttgaag
     5461 agaaaagaaa ttcaatatat taagtctaaa gtgacgcata ataaaaaaat ttcttatcaa
     5521 taccatgtag gacagtattt acccgactgg catccttggg aagattatcg caattttttt
     5581 gtttcaaaca aaaaaactaa tgggtgtaga gaaatattcg ctattgaatt accttggatt
     5641 atagatacat ttgggaaagt taaatcgttt actgtaatga cccagaaatt atccaatctt
     5701 gatattgatt attcggacag tttcactctt attattgagc atgaaactgg tatagttgga
     5761 actttaagtg tagatgttgt ttcaagagta gctaaaagaa atttaactat aattggtgaa
     5821 aagattttaa tagaatggga aggtaacccg gattctctat tttcatggaa tgaagaaact
     5881 tctagtatga cagatattca attgtacgat gaggttatgc atgataataa ttattcacat
     5941 actattattg aagatgcata tttagaggag attaaagaat ttttcacgct tttggaaaag
     6001 aaaatggaac cccaatatac ttttgaaaaa gataagtata ttattaactt aattaacgac
     6061 attgaggtaa attaatgatg aaagtaattt tttttgggtt agggtcaatt ggaaagagac
     6121 atctaaaaaa tctaataagc tatggaaaaa agaattctat taattttgat ataacagctt
     6181 atagtagcag tggaaaaaaa gagtttgaag aaataacgta tattcaaaat agagacgatt
     6241 tggatacaca ttatgatata gcttttatta caaatccgac ttttaagcat ctggaaacac
     6301 taaatgtaat tgttgaaaaa gttgattaca tatttttaga aaaacctgct ttttctgaga
     6361 gtattgatct tactcaatat aattatgatt acaaaaatat ttatgttgct gctccactga
     6421 ggtataaaaa tatcatggaa gttgttcgta atagtattca aagtaaaaaa gtatattctg
     6481 taagagctat ttgttctact tatcttcctg attggagaaa aggtactgat tatagaaata
     6541 attattctgc ctttaaatct atggggggag gagtagaatt agactgtata catgaattgg
     6601 attatattat aaacctattt ggtttcccta aatcagtaaa aaaacagttt ggtaaatatt
     6661 cgaatcttga aattaacagt aatgatttag caacatatat attgagatac gatgataaaa
     6721 ttatagaaat tcatttagat tattttggaa gagttcctac aagaaaattt caattgttta
     6781 ctttagatga atgtatcaat gttgacttat taaataataa aataagtttt gaagaaaaca
     6841 ttgttaaaga aatgcctgaa tctgctaatg atatgtatga gaaggagttg aaatatttct
     6901 ttgaaaacgt agtaacaggc aaagaaaact ggaatgattt atatcatgca aacgaagtat
     6961 tgaaaatagc tgaggagaaa taaatggaaa ttttatttac tatttgtggt agagctgggt
     7021 ctaaggggtt taaaaataaa aatttgaaat atttggcagg aaagccccta gtccatttcg
     7081 ctttagcaat gatagatatc tatcaacaaa aacatcctga acataattgc acgacttgtt
     7141 tgaatacgga cagtaatgag ttagtagaat tgacgactaa gttaaatccg tctgtgaaaa
     7201 ttgtagaacg taaacctgaa ttagctacag atactgtagg gaaaattgaa gtgatacgtg
     7261 attcattctt acaactagaa agagaagata atatatttga tttaattata gatttagatt
     7321 tgacttcacc tttacgtgaa gtttctaatc ttgaagaatt aatagatgaa catctaaaaa
     7381 atttagataa agacgtctac ttttcagttg tttctgcaag acgtaatccc tattttaata
     7441 tggtaaaaag aattgaaaat gaagttacta tagttaataa atcaagatac acatctaggc
     7501 aacagacacc tgaagttttt gaaatgaatg cctcaatgta ttcttttaaa cctgaatttc
     7561 taaaaaaaag taattatatt tttgatgggc aatgtgggat aataaaaatg agagattatc
     7621 taattttaga tattgatagt gaagaagatt ttaaatggtt agaatttttg tttcctaaat
     7681 ttttagaaga ttcttcggag cttcaagata tctataatta tttatatcgg aggtaaaagg
     7741 atgagctgtt atcttatcac cggtggggct ggcttcattg gttctacctt agccaatcat
     7801 ttaggagaag aacatgtggt aatcgtcgtc gatgacttat caatgggaaa agtagaaaat
     7861 cttgatatga cgaaaaatat cacttttatt gagggagatg tcgctgagaa atccttgatg
     7921 gaagagatca tgaaagctaa taaatttgat tatatctttc atttagcagc agttgctagt
     7981 gtggcagatt cagttgcccg accagtagaa acacatcgtg tgaattttga aagtgtatta
     8041 atgattttag aattgatccg caagtatcaa cctgatttaa aacggatcgt gtttagttct
     8101 tcagcggctg tttatgggga tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc
     8161 ccattgacgc cttatgcggt tgataaattc gctgcagaac aatatgtgct agattattgt
     8221 catttatatg atgtacccgg tagcgcagtt cgttttttca atgtctatgg tccaaatcaa
     8281 aatccgaatt ctccatacag tggtgtgatt tctattctcg tagatcgcta taagaaacaa
     8341 atcgtaggag aagcaacttc atttacatta tttggtgatg gaagccagtc tagagacttt
     8401 gtttatatag atgatgtaat ccaagcatta cttttagttg cgaatgagga agctgcactg
     8461 gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt gattcatgca
     8521 atcaatgaaa ttctcggtgt agaattgaca cttgaataca aagaagagcg ttcgggtgat
     8581 atccatgact ctctagcaga tattactaag atccagtcaa ttggctatca accgaaatat
     8641 ggtattttaa gtggaatgac taattatttg aaaactgaaa tcaattaaaa catactaaga
     8701 taagattaga gggtaaattc aggctctttg tcaataagga ctgatgagtt ttgtaaaatc
     8761 aaatctggga tagaa
//
LOCUS       DAZUKT010000104         8633 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000104 DAZUKT010000000
VERSION     DAZUKT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.107"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..536
                     /locus_tag="ACG816_002594"
     CDS             57..536
                     /locus_tag="ACG816_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4921435.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            753..1844
                     /gene="phnW"
                     /locus_tag="ACG816_002595"
     CDS             753..1844
                     /gene="phnW"
                     /locus_tag="ACG816_002595"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS4921436.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1837..1965
                     /locus_tag="ACG816_002596"
     CDS             1837..1965
                     /locus_tag="ACG816_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4921437.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1969..2514
                     /locus_tag="ACG816_002597"
     CDS             1969..2514
                     /locus_tag="ACG816_002597"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS4921438.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2968..4659)
                     /gene="argS"
                     /locus_tag="ACG816_002598"
     CDS             complement(2968..4659)
                     /gene="argS"
                     /locus_tag="ACG816_002598"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4921439.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5079..6026)
                     /gene="arcC"
                     /locus_tag="ACG816_002599"
     CDS             complement(5079..6026)
                     /gene="arcC"
                     /locus_tag="ACG816_002599"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4921440.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6141..7160)
                     /gene="argF"
                     /locus_tag="ACG816_002600"
     CDS             complement(6141..7160)
                     /gene="argF"
                     /locus_tag="ACG816_002600"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4921441.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7251..8480)
                     /gene="arcA"
                     /locus_tag="ACG816_002601"
     CDS             complement(7251..8480)
                     /gene="arcA"
                     /locus_tag="ACG816_002601"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4921442.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2478 a         1748 c         1589 g         2818 t
ORIGIN      
        1 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
       61 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
      121 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
      181 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
      241 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
      301 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
      361 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
      421 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
      481 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
      541 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
      601 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
      661 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
      721 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
      781 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
      841 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
      901 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
      961 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     1021 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     1081 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     1141 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     1201 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     1261 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     1321 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     1381 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     1441 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     1501 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     1561 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     1621 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     1681 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     1741 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     1801 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     1861 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     1921 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     1981 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     2041 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     2101 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     2161 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     2221 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     2281 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     2341 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     2401 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     2461 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     2521 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     2581 taacagaata cattttgtac gctgacatga atccagaatg acttttatac aaaataagag
     2641 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     2701 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     2761 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     2821 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     2881 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     2941 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     3001 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     3061 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     3121 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     3181 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     3241 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     3301 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     3361 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     3421 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     3481 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     3541 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     3601 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     3661 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     3721 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     3781 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     3841 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     3901 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     3961 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     4021 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     4081 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     4141 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     4201 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     4261 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     4321 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     4381 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     4441 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     4501 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     4561 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     4621 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     4681 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     4741 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     4801 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     4861 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     4921 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     4981 tagtcgtttc ataaaaaaac aatccagatg gcctaaagga gaagaaatac cacctggata
     5041 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     5101 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     5161 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     5221 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     5281 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     5341 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     5401 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     5461 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     5521 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     5581 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     5641 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     5701 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     5761 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     5821 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     5881 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     5941 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     6001 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     6061 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     6121 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     6181 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     6241 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     6301 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
     6361 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
     6421 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
     6481 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
     6541 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
     6601 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
     6661 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
     6721 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
     6781 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
     6841 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
     6901 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
     6961 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
     7021 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
     7081 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
     7141 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
     7201 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
     7261 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
     7321 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
     7381 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
     7441 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
     7501 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
     7561 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
     7621 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
     7681 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
     7741 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
     7801 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
     7861 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
     7921 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
     7981 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
     8041 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
     8101 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
     8161 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
     8221 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
     8281 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
     8341 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
     8401 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
     8461 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
     8521 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
     8581 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat tta
//
LOCUS       DAZUKT010000105         8586 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000105 DAZUKT010000000
VERSION     DAZUKT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.108"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1511)
                     /locus_tag="ACG816_002602"
     CDS             complement(45..1511)
                     /locus_tag="ACG816_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4921443.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="ACG816_002603"
     CDS             1898..2494
                     /locus_tag="ACG816_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4921444.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="ACG816_002604"
     CDS             complement(2552..3034)
                     /locus_tag="ACG816_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921445.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="ACG816_002605"
     CDS             complement(3140..3337)
                     /locus_tag="ACG816_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921446.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="ACG816_002606"
     CDS             3632..4390
                     /locus_tag="ACG816_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4921447.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="ACG816_002607"
     CDS             4450..4878
                     /locus_tag="ACG816_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921448.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="ACG816_002608"
     tRNA            4924..4996
                     /locus_tag="ACG816_002608"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="ACG816_002609"
     CDS             complement(5146..5517)
                     /locus_tag="ACG816_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4921449.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="ACG816_002610"
     CDS             5732..5917
                     /locus_tag="ACG816_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921450.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="ACG816_002611"
     CDS             complement(6000..6188)
                     /locus_tag="ACG816_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921451.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="ACG816_002612"
     CDS             complement(6212..6475)
                     /locus_tag="ACG816_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921452.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="ACG816_002613"
     CDS             6633..6890
                     /locus_tag="ACG816_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4921453.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="ACG816_002614"
     CDS             complement(6961..7152)
                     /locus_tag="ACG816_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921454.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="ACG816_002615"
     CDS             complement(7293..7613)
                     /locus_tag="ACG816_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921455.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="ACG816_002616"
     CDS             complement(7636..8070)
                     /locus_tag="ACG816_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4921456.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="ACG816_002617"
     CDS             8405..>8586
                     /locus_tag="ACG816_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4921457.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2719 a         1693 c         1361 g         2813 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctgaggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagtg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DAZUKT010000106         8546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000106 DAZUKT010000000
VERSION     DAZUKT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.109"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACG816_002618"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACG816_002618"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4921458.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="ACG816_002619"
     CDS             complement(1098..2201)
                     /locus_tag="ACG816_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4921459.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACG816_002620"
     CDS             complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACG816_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS4921460.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="ACG816_002621"
     CDS             3528..5462
                     /locus_tag="ACG816_002621"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4921461.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="ACG816_002622"
     CDS             5919..6335
                     /locus_tag="ACG816_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4921462.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="ACG816_002623"
     CDS             6349..6915
                     /locus_tag="ACG816_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS4921463.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="ACG816_002624"
     CDS             6938..7924
                     /locus_tag="ACG816_002624"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4921464.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8546)
                     /gene="scm"
                     /locus_tag="ACG816_002625"
     CDS             complement(8079..>8546)
                     /gene="scm"
                     /locus_tag="ACG816_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4921465.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2519 a         1644 c         1735 g         2648 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt tacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgtggt agaggtagta
     8521 tctgtactgt cacttgaagc tgatgt
//
LOCUS       DAZUKT010000107         8493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000107 DAZUKT010000000
VERSION     DAZUKT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.110"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG816_002626"
     CDS             complement(<1..149)
                     /locus_tag="ACG816_002626"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4921466.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG816_002627"
     CDS             complement(593..1714)
                     /locus_tag="ACG816_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS4921467.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG816_002628"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG816_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS4921468.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG816_002629"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG816_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS4921469.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG816_002630"
     CDS             complement(4304..5215)
                     /locus_tag="ACG816_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4921470.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG816_002631"
     CDS             complement(5232..6239)
                     /locus_tag="ACG816_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4921471.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG816_002632"
     CDS             complement(6236..8365)
                     /locus_tag="ACG816_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4921472.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG816_002633"
     CDS             complement(8370..8480)
                     /locus_tag="ACG816_002633"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921473.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAZUKT010000108         8424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000108 DAZUKT010000000
VERSION     DAZUKT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.111"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..505)
                     /locus_tag="ACG816_002634"
     CDS             complement(<1..505)
                     /locus_tag="ACG816_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921474.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILIS"
     gene            471..2108
                     /locus_tag="ACG816_002635"
     CDS             471..2108
                     /locus_tag="ACG816_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921475.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="ACG816_002636"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="ACG816_002636"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4921476.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="ACG816_002637"
     CDS             complement(3184..3819)
                     /locus_tag="ACG816_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4921477.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5186)
                     /gene="sstT"
                     /locus_tag="ACG816_002638"
     CDS             complement(3966..5186)
                     /gene="sstT"
                     /locus_tag="ACG816_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4921478.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5405..5767
                     /locus_tag="ACG816_002639"
     CDS             5405..5767
                     /locus_tag="ACG816_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921479.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            5754..>8424
                     /locus_tag="ACG816_002640"
     CDS             5754..>8424
                     /locus_tag="ACG816_002640"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4921480.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaa
//
LOCUS       DAZUKT010000109         8193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000109 DAZUKT010000000
VERSION     DAZUKT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.112"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..604)
                     /locus_tag="ACG816_002641"
     CDS             complement(74..604)
                     /locus_tag="ACG816_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4921481.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(786..1442)
                     /locus_tag="ACG816_002642"
     CDS             complement(786..1442)
                     /locus_tag="ACG816_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFS4921482.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1547..2042)
                     /gene="dfrF"
                     /locus_tag="ACG816_002643"
                     /pseudo
     CDS             complement(1547..2042)
                     /gene="dfrF"
                     /locus_tag="ACG816_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
     gene            complement(2437..3828)
                     /gene="amt"
                     /locus_tag="ACG816_002644"
     CDS             complement(2437..3828)
                     /gene="amt"
                     /locus_tag="ACG816_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4921483.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            complement(3914..4141)
                     /locus_tag="ACG816_002645"
     CDS             complement(3914..4141)
                     /locus_tag="ACG816_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921484.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(4169..4411)
                     /locus_tag="ACG816_002646"
     CDS             complement(4169..4411)
                     /locus_tag="ACG816_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4921485.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4443..5000)
                     /locus_tag="ACG816_002647"
     CDS             complement(4443..5000)
                     /locus_tag="ACG816_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4921486.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5013..5201)
                     /locus_tag="ACG816_002648"
     CDS             complement(5013..5201)
                     /locus_tag="ACG816_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4921487.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5214..5780)
                     /gene="amaP"
                     /locus_tag="ACG816_002649"
     CDS             complement(5214..5780)
                     /gene="amaP"
                     /locus_tag="ACG816_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4921488.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            6197..6370
                     /locus_tag="ACG816_002650"
     CDS             6197..6370
                     /locus_tag="ACG816_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921489.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            7010..7483
                     /locus_tag="ACG816_002651"
     CDS             7010..7483
                     /locus_tag="ACG816_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4921490.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7492..7719
                     /locus_tag="ACG816_002652"
     CDS             7492..7719
                     /locus_tag="ACG816_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921491.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2517 a         1615 c         1204 g         2857 t
ORIGIN      
        1 aaatcttaag gattttcccg ccaacgtggt cggatcgctc cgtcggttta acagcttttc
       61 tgtttctctc tagttatcta cgcatataaa tcaaatcggt tattccgtta taagtctgtg
      121 ttttacaaag ccttaacttt atttccctgt ctatgttgcc caaaagacga accccatttc
      181 ctaacagagt aggaataaca gaaatgtaat atttatcaat catatttttg cttaccaact
      241 gctgaacaag atttgctccg ccacaaatcc aaatatcctt tcctgcttct tgttttagcc
      301 gttccaataa taaaacagga ttttcacttg taaaatgaat ttgttgggaa gaattgcact
      361 ctttatgtgt taatacataa gttgtaaatt tattatatac ccattcttgc ggagaaagtt
      421 cagtaacaac ctgatgagaa gtattccacc ccattaagac agtatctata tctttggtaa
      481 attctgtata agtgtcgatg ttttcattgt catttccatg accatttagc caagctactc
      541 caccattacc gtccgcaata tatccgtcaa ggctcatggc aataaataaa actacttttc
      601 tcattttttc tcttttataa atacaaacag gaatttattt ctctttttta ctcataaggg
      661 aacaactgat tgctacaaat aaacccatta aaatccatgg aaatgctgct cttataaaat
      721 cactaaccat actttattcc tccttaaatt ccaatttcct tagttctaca aactgaaatt
      781 tttgcctatg aatgtatctt gttctttata cgcaaacagg cttctgtgtg cataatccaa
      841 tgtcttgaaa acggcatttg aattagtcca caaatatctt tattacacca ataatcaatc
      901 agccaaattg ctttttcatt gtcgtttact acattcaacg attctaagta tccttttctt
      961 tctttcggta tttttctttt caattcatca taagataacc gttcaagcat tttctcagtg
     1021 ctttcccata cttcgaaaat atataaatat aattcttcca gattaagctg ttttgaaaag
     1081 tctgcaatct gctgtttcat gagttcattt cctgttgtaa tgataggtga attgatacgc
     1141 tcttgataat tgcctgcaaa aaatacttgt tcatcttcat ttatcactgt atgcacaact
     1201 atatcttcaa tgcggaaaat atgccaaata gaataagcaa tcgtcttact atgataacca
     1261 tcagcgttta tgaaggggat tgagttaaaa tcttctctgc ataattcttc tttgaaagat
     1321 actaaggttt gtattaactg acttcgtaaa gtaagtaaag tatcaatacc cctcttataa
     1381 gtatctttct tttttatttg tgcctgcatt gttttattaa gttcagacca ttccttattc
     1441 atattcaatc ctctctcaaa ttctgatttt atcgccccat acaaactgga atttgtctta
     1501 caacaattta tatatgcata aaaagcaata tatttatgaa aacaccctaa atccaaaatt
     1561 tactcaattc attttttcct tacataaaat gttctcgtat atttcacttc ctcaccaagt
     1621 gtttccccta tcaaaacttc aaaatcattg atatcaaatt ctggaaagaa tgtatctcca
     1681 tcttcaatat ttaaatctac ttctgtgata tacattttat ctactatttg caaagcttcc
     1741 ttaaatagtc catatccacc agatatgtat acatctcgtc ctttagccaa taataatgca
     1801 tcttctaatg atttaactga aactaaatta tctccttgat actctgttgt ggtggaaaca
     1861 acaatattca ttctattagg caacggatga ccgatttctt cataagactt tcgccccata
     1921 ataaccacat tacccgttgt taactctcta aattgctttt gttctccctt tattttccat
     1981 ggtatattac cattcttgcc tataacatta ttctttgacc tcgcaacaat caaacctatc
     2041 atctatttca tcctccataa tatgatgtcg tacctgtgat tccatcaagt gcaccacatc
     2101 ttaacaaact caaaattatc tcgaactaac cagctcgaca aattcctttt ttgtcttcat
     2161 ctgcattata tcatattgtt ataagcattg ccatttcttt tctgcttttg aaaagttact
     2221 tatcagccta tgttttgtaa agggtgatat tcagcatttc tcacgcaaca agcccttgac
     2281 aaaccataga ctaataagtt ttaaggtaat caagcagaaa tgaaatagtc tgctgtttca
     2341 cggcaacaaa aaagagatac caacacactt atgtagtatc tcttagtcta gtagcaatat
     2401 tcttttgttt gcaagcaggt aaacatgggc tgtttcttaa tatctttttg tttgggaaac
     2461 acctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
     2521 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
     2581 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaagaa
     2641 aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc
     2701 cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat
     2761 cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc
     2821 aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc
     2881 aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc
     2941 cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat
     3001 ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt
     3061 tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc
     3121 aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca
     3181 gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat gctgacttct
     3241 ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc
     3301 agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa
     3361 tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac
     3421 aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac
     3481 ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc
     3541 atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc
     3601 aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat
     3661 cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg
     3721 tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca
     3781 taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc
     3841 ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa
     3901 ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt
     3961 ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt
     4021 tattttcgtc tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat
     4081 ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca
     4141 tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat gaaacgacta
     4201 atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca attgttggtc
     4261 cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca atgatgtttg
     4321 cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct ccaatgatac
     4381 cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg acttcttact
     4441 tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt gcctacagca
     4501 gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc tgcttctgtt
     4561 actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt gatgtctgca
     4621 acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc agtactgatc
     4681 aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac gatcacatct
     4741 agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt tacgttatct
     4801 gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt caatagacca
     4861 tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc ataagtcaat
     4921 tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc atttgttgta
     4981 ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg atcaactatt
     5041 cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat tatgatcaac
     5101 aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc aagaccccca
     5161 aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc ctcctattcc
     5221 actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct gtatgtgact
     5281 gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc gatatcttgc
     5341 atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg
     5401 atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat gaactcttct
     5461 tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt tagtttgcca
     5521 tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac gactagcaag
     5581 atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg ttgcatgtag
     5641 taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat atacggcgca
     5701 ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat aacaccgatt
     5761 atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc tgtaaataac
     5821 tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta aataaagcgc
     5881 taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg aaataacaat
     5941 tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat attattcaag
     6001 attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta tttattaaat
     6061 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
     6121 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
     6181 aaatgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
     6241 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
     6301 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
     6361 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
     6421 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
     6481 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
     6541 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
     6601 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
     6661 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
     6721 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
     6781 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
     6841 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
     6901 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
     6961 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
     7021 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     7081 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     7141 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     7201 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     7261 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     7321 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     7381 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     7441 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     7501 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     7561 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     7621 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     7681 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     7741 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     7801 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     7861 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     7921 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     7981 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     8041 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     8101 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     8161 tactatgtag ttttgataaa atgatttgaa taa
//
LOCUS       DAZUKT010000110         8125 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000110 DAZUKT010000000
VERSION     DAZUKT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.114"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..144)
                     /locus_tag="ACG816_002653"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="ACG816_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="ACG816_002654"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="ACG816_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4921492.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1383..1829)
                     /locus_tag="ACG816_002655"
     CDS             complement(1383..1829)
                     /locus_tag="ACG816_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921493.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYWC"
     gene            complement(1822..2136)
                     /locus_tag="ACG816_002656"
     CDS             complement(1822..2136)
                     /locus_tag="ACG816_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921494.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(2352..3743)
                     /locus_tag="ACG816_002657"
     CDS             complement(2352..3743)
                     /locus_tag="ACG816_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4921495.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3728..4339)
                     /locus_tag="ACG816_002658"
     CDS             complement(3728..4339)
                     /locus_tag="ACG816_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4921496.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(4343..4540)
                     /locus_tag="ACG816_002659"
     CDS             complement(4343..4540)
                     /locus_tag="ACG816_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921497.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(4531..7209)
                     /locus_tag="ACG816_002660"
     CDS             complement(4531..7209)
                     /locus_tag="ACG816_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4921498.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(7196..7414)
                     /locus_tag="ACG816_002661"
     CDS             complement(7196..7414)
                     /locus_tag="ACG816_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921499.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(7490..>8125)
                     /locus_tag="ACG816_002662"
     CDS             complement(7490..>8125)
                     /locus_tag="ACG816_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4921500.1"
                     /translation="KITHQENRQKNLIVQNLNYGTNQYSVDSSKFELCDTSMKTRDKN
                     NFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIEENLLKQI
                     WGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHV
                     REQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
BASE COUNT         2295 a         1546 c         1165 g         3119 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc agtagtcctg cctttcaatc ggtaattcat aatagtaatt tactgtttct
     1441 tctttgccac tttcaatata ctctggatta tccaatatca tatcatactc ataaagttct
     1501 tcaccatcct tattttcaat cgaccattgt tgtggacgta tcggattttc tgtatcattt
     1561 ataagtgtat aggtcacttg taatacacga tcatgttgtg caaattcatt tcttttttcg
     1621 gtcacttcaa tatttttaaa tgttagttca accccatctg cctgataagt ttcacctatt
     1681 tttaaatctt ttgttacaat ttttattggc ttttcttctg gaaaatgtct ggctttaatt
     1741 gttagataac ttccaaaaag aatcaatcct agtagcacta aaagaattcc tttaacgagc
     1801 cactgaaacg ggaagtaaac tttaaacatc ttttttctcc tccgttttgt atttgttttt
     1861 tagagaatac gataactttt gatcaatgtc atccagcttt tcaattactt cattacttat
     1921 cattgattct agttctgaaa ttttttgttc aaatttaatg ctttcaggat cattaaattt
     1981 attatttgat ggactagcta acactggtaa atttgaaacc ataacacgaa acatatttgc
     2041 ctttgaacca aaaattcctt tgttttggtt gtaaaaattg ttgaatattt ctttagtttc
     2101 ttctgaagcc gagactttaa aatatggata gttcattttt ttcttccacc tttctttttt
     2161 tgggagggag tctgtaggga gcttgctccc tcagagattt acccgccatc tgacttgcca
     2221 gaaagtagcc tagcgtgagc ggggcggatt gactggcaag tagatgggta gctggcgtat
     2281 gtcctaaaaa ctgcttcttt ccaagcaact cttcataggc aaaaaactgg aaagtccact
     2341 aacaagccca ttcagaattc catttcaaaa ttttgttgcc ttttttgctg attttcaatc
     2401 atttgttgtg tgaattcata atccttttta gcacgataca tttttacatc gtcattcatt
     2461 atgcgactga tttgatagtg aatctgttta gcagaaggtg tatgagaaca attttcaaaa
     2521 ccctttgccg gtagaaataa ctctttctga tcagtctttt taatagaaat tgttttactt
     2581 ttcgcactac gtaaagtttg aggattcaca tccgtatgaa gtttaatcgt gtattcttga
     2641 tcatcttcta aagacattgt atttacaaaa tgcttgtcta cctctcgcaa gaagtcatca
     2701 tatttaatga atttttcatc gccttcacgg tcgctaaatt gcttttgtgc gatggaatct
     2761 aattccacaa acggtacatt taactcacca gttttgtaat ttactccact aattagcgac
     2821 ataacttgct ctctttccat attaaaaaaa ttttttatat gtgattcttc taaattcttt
     2881 tcaagttgtt cagaaacaga ttcaaatgaa tatcttttcc ctaactttct tgcactaaaa
     2941 gtacgttcga catactgact tttttctttt ctagattgtt tatctcttcc taaagtaatt
     3001 aactcacctc tacgctcata gaaatttaca ccttgtttgt aaagaacata gccaagtttg
     3061 ttatttgttt ttgaaaattg ataaacttca atgttatatt cttttgccaa atgatccgaa
     3121 aaatcttcaa aagaattcac ttcgcatttt tccaacgttg cttcgatcgc attttgcaca
     3181 aactcttgat aattaatttt tccatatttc atcttagaag gatcgtttga ataaagattg
     3241 gttctctttt ctaagttttc atagtactca ttattcactg attcaagacc atgttcattg
     3301 aggacttgat cgttaatttt tcgcattagt tctaaatctt tagggtcaat acgcaacctt
     3361 ttacatgttt ccatgttaat attattaatg acaagatgac tatgaaaatg atctttatca
     3421 ttgtgacttg caacaatgat ttgcgaattt ggaaaagctt tctctgcaaa ttcgacagct
     3481 atttcgtgca ttttatcaat acttaactct ttagaagtac tcttatcaaa cgaatgaata
     3541 acatgaaacg cttgtgctat atctttagat ttattcatct tggtacgtac taaattcatt
     3601 tcttgcagtg cttcctcact atcatcacta caatttattc ctgagactgc aacacatttt
     3661 tctttttttg aaggattagc tgcatattgt aatagcgagg ataaattttt cttgcgtcct
     3721 ttcgatgtta tagtagctcc caaagttgtt tcacctcatc ttttaaactt gcaataatct
     3781 gaattgagtg aattaaattc cgatcaactt tttgggaata ctcttctttc gtacccggtc
     3841 tagtttgctt ttctatccaa tattcgaaat attctttttc tacttctgat aatttaaaaa
     3901 aagagggggc actccgattg gactccaaaa aaatgtcatc tggataacta atcatcaact
     3961 tttttgctat gctattcgca agttcatctt taaaaatgtt gtaacggcta gcatcaaaac
     4021 taaaatgtag gctcttttct tttttatctt cagattcttc tctaaaatat ttagcaatga
     4081 tatttaatgc ctttaccgct tgattcaaat tgttaccaat ttgatttaac tgtttggtta
     4141 aaactaccct atcatcttca gctaacaacg gcggttcata tttgagtccc tttaaccgct
     4201 cacggcaaac gtctgctcta gaaattcccc tttgttttgc aatcaatgtc agttcaagat
     4261 aagtcttttt atcgacacga aaatttaagg cctcactatg tttgcgattt ttttcgtaca
     4321 ttttttgttt atcatccatt gattatcccc tcacattttt ttctagagag caacatttga
     4381 aattgctctc tatttcattt ttcatcttta attcttcttg aatcatcagt tcaatataat
     4441 tagtgaccgt gattttttca tttgttgcgt tctttactga ttcacataat tcgtcaagtt
     4501 ctaagaaatc atgcgtacta atctttagaa ttaattgcat gacaatcccc cccttttatt
     4561 tcttaataaa ttatcgaaaa gtggattagg atcattaagt gtatctctga ataactcatt
     4621 gtcttcataa gtgaatcttc gtcttgattt tgatccctta tcattttcac ggatttcact
     4681 atcaataatc actgtttcat gatttgaatc aaattttttc gctgactttc tgtcttcaac
     4741 gatattgatt ttttgtgttt tttctttcat ttctccacta tctttttgaa tgttctcccg
     4801 aacatctact gttttgaatc cttgactact tgtagaactt tgtacatttt ctcggacatc
     4861 tactgttttt ggttgacttg cactgctacc ttgcacattc tctcggacat ccactgattt
     4921 aaacccttga ctacttgtag aattttgtac attttctcga acatctactg tttttggttg
     4981 acttgtactg ctactttgca gattttctcg aacatcaatt gtcttagtac cttgagcatt
     5041 tgaagaactt tgtaaattct ctcttacatc aacattccgt ccaacatgac cattcagtga
     5101 accttgaata ttttctcgga catctactga tcggccacca tgactacttg tagaattttg
     5161 tggattttct ttgactaatt tttctaatcc tgtatttgaa tttttagctt cttgaattct
     5221 ttctttggtc aacgcttctt ttccgtcaat attagcgcct gtccgccctt ctacacgttt
     5281 ccttaacatt atttcctcgc gattcattgc ttcaggcgtg tatttcgctt catgacgctt
     5341 ctgattaatt aattcttcag cagacttagc ttgaccgtgc atagcatttt tacgttcttc
     5401 aatatcttgt tggacttttt gctgaattgc gcttgcagga ttattattcg attcagcatt
     5461 atttttcatt gcttcacgca tacgttcaga actagacata attctatcgg cattttcaac
     5521 tttagcacca tcttttctat ctgaaagacg tgttggcacg gaatcagcat attttccagt
     5581 ttcatcttct ttatttttag ctaacatagg tttgctattt ttattaatag cggacactgt
     5641 accatcatct aaaccttctt taaacttatt cttggtatca ttagctatat cacgcatact
     5701 cttaaatggt tttgttgctc tttcactagc cattgtagct ccatctgaag ctaaaccgga
     5761 taatccacgt tcatgggcat atcccaaagc tcttccagtt ttgccagctt tttttgatag
     5821 agtttcgccg aaattctttt ctggttttcg atcttctcct tctccgcttc ctttagcacg
     5881 attaaatcca tttgatcctt ttctaaacag cattgcgccc attccaaaag cagaagcaag
     5941 ttgcccatag ccttctttga gtccaatatc aacaccaaag tatctcatga ctgttccact
     6001 gcctttgatt aaaacaaaaa cggcacagat ataagcaatt gtttttataa aaatatttgt
     6061 tgagatacct tgtcctgagt tcagaaaatt gataaacata gcaaacattg ataacccaaa
     6121 tccttgaaac ccaacagtta agtaacattg tagaatatct gatagaacaa cttttgaacg
     6181 ctgtcctgtc tcaatatctg ttgcaaaaac aagaacacct aacacacgtt taaatgcaag
     6241 ttctagtatt gctgtaatga tcacaaacgc gctgaacaca taggcgattg ctaacgctgt
     6301 caacccaatt gaaatccccc atagatttgc ttggtatcgg taatagcctg atttcaagct
     6361 atcagaaaat gttgataaga agttgtcaga aaactttgta gcaacaaatt cattattaga
     6421 attttcaact aattcataac gaagattgtc cgcaattgga ttttcttttt ccaatttatc
     6481 aatcacttta tccgttaaca cttgtgtgag atccgtctgt tgaaaatttt ttcgtgttaa
     6541 attatttcga tcctccgttt tatcaatgct tgagtactga tctgttttat taatatatgc
     6601 taaatctgtt actccttgtt ttatcaatga ccaagcgact ccgtcatctg aatttgtaat
     6661 cgtagtagag tccttataaa atgttttagc aaattgaata ccactagaaa tcatggttgg
     6721 cattaaaagt attaatgcta tagacatcac tacgttcatt ccaaccgatt ttaaatccac
     6781 tgttcctttt ccagtaatca ttttgtaacc aacaaacatc aatgaaagga tcattaatcc
     6841 aatgacaatt gtatttacgg ctgattgata aacattattt agccctgtac tttcaatgaa
     6901 attgaacaaa tccagcacat ctggaatcat attttgaatc gaatctgtca cagtaaacat
     6961 gccttttacc acagcccatc cgagccaaat cccgaactga ctaaggaaat ctccattgta
     7021 cttttttagg taaggtctat aaagagaatt attcaaaaac tctgtttgac tttttgtgtc
     7081 atcaaaccaa ttgaaaatac tcgcatgagc aatattactt gtaaaaataa tcatagctat
     7141 cagaacaaga aaattaacaa ccaaccaatt tattttttta ttggcaatct ttttttcaat
     7201 tattttcatc gttacctccc ttagcccatt ttctgaggct ctcagagagc ttctgagcca
     7261 ttttttgatt aattatgaca tgagttacac aattatcatt ttcagctctt aaatcgagaa
     7321 ctatagtgtt gaagcaaata gtctcgatta attcgagact atctgtattt tgatctagac
     7381 accaaacttg atctattaaa cgatttttaa tcaaaataac ccctcctata ttcaaagttc
     7441 tcaaatgaag tggagatacc cctccatttc atttgactca attctattct tattgcttat
     7501 ctattccttc gctgatttgc aattcaatta gttctaacgc tttatttttt agagaagcaa
     7561 agatataatc gtctttttgt ttgatacgat gatctgtttt tgctcttcgt atcaagtcac
     7621 gtaaagtcat tgatagctgt tctctaacat gatctacaaa atacttgtta ttacaaattt
     7681 catagataat tttcagttga attttttgtt ctttttcagc agatctaatc gcatatttga
     7741 attttttgta ccagttgtcg aattgacgat aatcactatt gctagccagt tttaagcaat
     7801 tcacaatact ttctccccat atttgtttaa gaagattttc ttcaataaat gtttcgatca
     7861 attcttcatc taatttatta tttttagttg aaagtgattc aagaagatca tcattatgtg
     7921 attctttaat atctttacta tctttataag acttatataa ttctagttca ttttttgaac
     7981 tatcagcaaa attgtttttg tctcttgttt tcattgatgt gtcgcatagt tcaaattttg
     8041 aactatcaac agaatattga tttgttccat agttcaaatt ttgaactatc aagttttttt
     8101 gtctattttc ttgatgggtt atctt
//
LOCUS       DAZUKT010000111         7599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000111 DAZUKT010000000
VERSION     DAZUKT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.116"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACG816_002663"
     CDS             complement(137..427)
                     /locus_tag="ACG816_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921501.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACG816_002664"
     CDS             complement(545..805)
                     /locus_tag="ACG816_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921502.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACG816_002665"
     CDS             complement(942..1355)
                     /locus_tag="ACG816_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4921503.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACG816_002666"
     CDS             complement(1811..2296)
                     /locus_tag="ACG816_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921504.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACG816_002667"
     CDS             complement(2435..2650)
                     /locus_tag="ACG816_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4921505.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACG816_002668"
     CDS             2874..3674
                     /locus_tag="ACG816_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4921506.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACG816_002669"
     CDS             3693..5561
                     /locus_tag="ACG816_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4921507.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACG816_002670"
     CDS             5554..6045
                     /locus_tag="ACG816_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4921508.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACG816_002671"
     CDS             6033..6587
                     /locus_tag="ACG816_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4921509.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="ACG816_002672"
     CDS             6605..>7599
                     /locus_tag="ACG816_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4921510.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAZUKT010000112         7534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000112 DAZUKT010000000
VERSION     DAZUKT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.117"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG816_002673"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG816_002673"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4921511.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACG816_002674"
     CDS             complement(2032..3285)
                     /locus_tag="ACG816_002674"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4921512.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACG816_002675"
     CDS             complement(3291..4214)
                     /locus_tag="ACG816_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4921513.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACG816_002676"
     CDS             complement(4435..6927)
                     /locus_tag="ACG816_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4921514.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACG816_002677"
     CDS             complement(6934..7401)
                     /locus_tag="ACG816_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4921515.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2075 a         1638 c         1208 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAZUKT010000113         7507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000113 DAZUKT010000000
VERSION     DAZUKT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.118"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG816_002678"
     CDS             <1..390
                     /locus_tag="ACG816_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921516.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            complement(506..2059)
                     /locus_tag="ACG816_002679"
     CDS             complement(506..2059)
                     /locus_tag="ACG816_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921517.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            2207..3496
                     /locus_tag="ACG816_002680"
     CDS             2207..3496
                     /locus_tag="ACG816_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4921518.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3622..4701)
                     /locus_tag="ACG816_002681"
     CDS             complement(3622..4701)
                     /locus_tag="ACG816_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4921519.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4810..5250)
                     /locus_tag="ACG816_002682"
     CDS             complement(4810..5250)
                     /locus_tag="ACG816_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4921520.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(5307..5717)
                     /locus_tag="ACG816_002683"
     CDS             complement(5307..5717)
                     /locus_tag="ACG816_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4921521.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5898..6332)
                     /locus_tag="ACG816_002684"
     CDS             complement(5898..6332)
                     /locus_tag="ACG816_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4921522.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(6432..7280)
                     /locus_tag="ACG816_002685"
     CDS             complement(6432..7280)
                     /locus_tag="ACG816_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4921523.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2701 a         1365 c         1235 g         2206 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccaattcttg atacataaat ccggaggttg ttaataagca
      481 ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat
      541 ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt
      601 tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca
      661 attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta
      721 ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat
      781 aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat
      841 acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa ataactggta
      901 acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt
      961 ctttcaacac gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat
     1021 tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct
     1081 tttctttttc aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg
     1141 aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt
     1201 cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt
     1261 cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa
     1321 tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct
     1381 ctttacatat ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa
     1441 gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat
     1501 aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa
     1561 tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac
     1621 cgtacaatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat
     1681 agtttgtttg ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca
     1741 agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag
     1801 ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt
     1861 ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg
     1921 attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag
     1981 ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa
     2041 tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa
     2101 tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct
     2161 actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac
     2221 catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca
     2281 cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat
     2341 tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca
     2401 aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta tactacaatc
     2461 agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg agaacacttt
     2521 gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt
     2581 aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatcattt
     2641 aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact caggaggaga
     2701 tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac
     2761 agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga
     2821 aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag aaaaaccaga
     2881 tgtagaccca gaagaaaaac cggatgtgac tccagaacca gatacggatt ctggaaacca
     2941 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
     3001 ggtagcccca gaagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
     3061 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaactaga
     3121 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
     3181 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
     3241 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
     3301 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctag
     3361 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
     3421 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
     3481 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
     3541 tatccagaaa acgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
     3601 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
     3661 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
     3721 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
     3781 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
     3841 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
     3901 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
     3961 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
     4021 caacgtgttt aaaagagcga taacaaattg agcttctaat accaaaccaa atgtattgac
     4081 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
     4141 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
     4201 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
     4261 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
     4321 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg actggatcat
     4381 atcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
     4441 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
     4501 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
     4561 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
     4621 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
     4681 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gaaagagcaa
     4741 aaagaaaaac cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat
     4801 ctagcagttt cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag
     4861 atgttgcagt gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg
     4921 aataccttct gcttgttttt gcaacgcagc accttgagga gtcagcgtaa catttacgat
     4981 tctttcatca cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag
     5041 taagggagtc agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt
     5101 ctttttcttt tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg
     5161 cttgaggagg gaagtgtagt gtctaatgat ttccttagag gcggcatata aaggaaaaca
     5221 tacttgatta actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt
     5281 atcatagcgg atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata
     5341 tttccagctg tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc
     5401 gtttcttcca gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc
     5461 gtttcacttt ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt
     5521 ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac
     5581 aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg
     5641 aatgtacgat cgggattatg aacttctcca gagcgtccgc ctgtgttgat cattttcgta
     5701 gaatacattt ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac
     5761 acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca
     5821 tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaaatatcag agacaaaaac
     5881 taatctcatc ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaactt
     5941 acttcacgat ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag
     6001 ttagtcacac caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt
     6061 aatgctttat tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt
     6121 ggatgttcag cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat
     6181 ctcgtgccat acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca
     6241 atcagttgta tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt
     6301 aatacaaaat ctatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac
     6361 agttactaga ttcttttatt ataccagtta aagaaaacgc attcaaaaaa atagcggatt
     6421 tacttaccaa ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg
     6481 ctgctgacaa tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa
     6541 aattcatgat cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct
     6601 tttgcctcta gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct
     6661 tccaaataat gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata
     6721 taagtcatga caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt
     6781 ccaaaaacaa acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa
     6841 gtattcaagt ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa
     6901 gagttgtttt tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca
     6961 gacaacaacc gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa
     7021 aacaagcttc cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt
     7081 accaacataa cgatattcaa cactaaaata actatagctc ggtcaaatcc taaaacagct
     7141 tccagtaata ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac
     7201 atattgatag cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata
     7261 aattgtctcg tttctttcat ttatccactt ccttaattca ttaataatac tcttatctta
     7321 ttataactta cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa
     7381 attattatgt ttcattcttc tagaatatac ttctacgtta aaaactgtta aaaaactttt
     7441 actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag
     7501 gaagtag
//
LOCUS       DAZUKT010000114         7327 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000114 DAZUKT010000000
VERSION     DAZUKT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.119"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            346..615
                     /gene="rpsN"
                     /locus_tag="ACG816_002686"
     CDS             346..615
                     /gene="rpsN"
                     /locus_tag="ACG816_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4921524.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            630..809
                     /gene="rpmF"
                     /locus_tag="ACG816_002687"
     CDS             630..809
                     /gene="rpmF"
                     /locus_tag="ACG816_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4921525.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            841..1218
                     /locus_tag="ACG816_002688"
     CDS             841..1218
                     /locus_tag="ACG816_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921526.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1239..1388
                     /gene="rpmG"
                     /locus_tag="ACG816_002689"
     CDS             1239..1388
                     /gene="rpmG"
                     /locus_tag="ACG816_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4921527.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1412..2395
                     /locus_tag="ACG816_002690"
     CDS             1412..2395
                     /locus_tag="ACG816_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4921528.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2425..2547
                     /locus_tag="ACG816_002691"
     CDS             2425..2547
                     /locus_tag="ACG816_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4921529.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2565..4088
                     /locus_tag="ACG816_002692"
     CDS             2565..4088
                     /locus_tag="ACG816_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4921530.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4112..4351
                     /locus_tag="ACG816_002693"
     CDS             4112..4351
                     /locus_tag="ACG816_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921531.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4544..5107)
                     /locus_tag="ACG816_002694"
     CDS             complement(4544..5107)
                     /locus_tag="ACG816_002694"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4921532.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5200..5418)
                     /locus_tag="ACG816_002695"
     CDS             complement(5200..5418)
                     /locus_tag="ACG816_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921533.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5607..6554
                     /locus_tag="ACG816_002696"
     CDS             5607..6554
                     /locus_tag="ACG816_002696"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4921534.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6521..6610
                     /locus_tag="ACG816_002697"
                     /pseudo
     CDS             6521..6610
                     /locus_tag="ACG816_002697"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6743..7150
                     /locus_tag="ACG816_002698"
     CDS             6743..7150
                     /locus_tag="ACG816_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4921535.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2589 a         1089 c         1437 g         2212 t
ORIGIN      
        1 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
       61 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
      121 agtgaattct tatttttcag agcttaaact gcatattctt ttaaatgatt tgattatctc
      181 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
      241 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
      301 acgatttgag atgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
      361 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtacgcaga taaacgtcag
      421 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
      481 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
      541 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
      601 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
      661 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
      721 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
      781 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
      841 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
      901 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
      961 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
     1021 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
     1081 gaggagttat ttcaatgtat tgatggttat tgggagatac ctgttgatat gaaagaaaat
     1141 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
     1201 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
     1261 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
     1321 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt ttcaaagaag
     1381 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
     1441 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
     1501 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
     1561 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
     1621 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
     1681 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
     1741 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
     1801 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
     1861 catagcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
     1921 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
     1981 tgaaacgatt caaaaaaagg atttttataa tttggaaaaa ttcaatcatc ccttagtttt
     2041 tgaaagtgat tcatcagaaa atcactataa tggcaaacac tatcatcaac atcatcaaga
     2101 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttatt
     2161 ccaattaatt gaccggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
     2221 gattgctttg caagatcaag attttatagt tgccatacaa ggggtaaacg aacaggttaa
     2281 ttttcaatac accactcgac aaaaagacca aaccactatt attctaattg gaaaaatgtt
     2341 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
     2401 agtattgatt aggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
     2461 cttaaaagcc cgaatatcaa aactagaaaa agggcattaa aaattatcaa agaacataaa
     2521 caaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
     2581 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
     2641 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
     2701 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
     2761 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
     2821 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
     2881 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
     2941 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
     3001 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
     3061 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
     3121 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
     3181 tgacacaaca tgcagccttt ggatatttag ctttggatta tggattgata caagttccta
     3241 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
     3301 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaatcg
     3361 caaaaacttt ggccaatgaa gctgggataa aattggaagt attgaatcct cttgaaagtc
     3421 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
     3481 aagcgttaga gaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
     3541 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
     3601 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
     3661 atgaagtttt cgattacaaa gcaaaactca ataaagacat gacagctgcc gagtacaaag
     3721 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
     3781 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
     3841 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
     3901 gtgctggtcg ttttaaatat attcaattta gcgatcatgg aattgcacca agtaaggcag
     3961 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actttataat gaaatgcata
     4021 attggcctac tttctatcca gcttcattat ctgagcatga gattgctcaa gaaatgatgg
     4081 cccattaaaa tccaatcttt ggaggaaata catggtaaga aaagtattat tgtctatgga
     4141 ttccaagaat attgatgctt atcggcgtta tcaatttgca aagcaaatga gattaaatgg
     4201 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
     4261 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
     4321 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
     4381 cttatttgga tttgatctgc tttttattat ttcttgaatt gtcaaatcaa gcgcaacact
     4441 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
     4501 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
     4561 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
     4621 gaggatctgg aaaataaaag ggcatattat tcaatacttc tgtaactgcg aaatgattgg
     4681 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
     4741 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
     4801 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ctagttttgc
     4861 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggat
     4921 ggccatggat cgaatgagta aggcaaatct tcccacgaga ctcgacatga ttttttgtat
     4981 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
     5041 gttctgtatc gaaaagatca ttgcaaattg cacggtagat cgtgataaaa ctaattgaaa
     5101 agtccatatg ttcatgcttc aaacgattcg agattttctc tggcgaccac tgaaaatcga
     5161 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
     5221 atttttttct tcgataagca taattttttt ccgctttgga aggtgattag tttttttctt
     5281 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
     5341 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgtttga gtatagtaag
     5401 atgacaatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
     5461 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
     5521 agaaagaggt tccctatgaa aaaataccta ttacaatctt aactggctat cttggttcag
     5581 gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa attgctgtca
     5641 ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag aaaggtttta
     5701 aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt acattacgcg
     5761 aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat caaattgtta
     5821 ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata ttagctaaaa
     5881 accctttaga cattgatttg acccaacgat tagagattgg tgccattatt atagtagtgg
     5941 atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg
     6001 aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat caattcgagt
     6061 gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg atttacaaaa
     6121 ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca attaaaggtg
     6181 aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag acttcctatt
     6241 caatgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct gaaacagaag
     6301 aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca caaaaatttg
     6361 cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg tttctttggt
     6421 ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagccat aaaagtattg
     6481 aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg attatacaaa
     6541 aaaacccgaa ttgaaggaag cctggcatcc gttgtttggt gaacgcatta ataaactagt
     6601 ttttattggt atgaagatgg atcaacaatg tattaaggaa caacttgagg gttgtttaac
     6661 tgattctctt aatgcagata atttccgaaa aatttcacca tttccatgga aaaattctaa
     6721 tatgtttagc cagatatcgt taatgaaatt tttaagaaaa gctggtgtca agataggcga
     6781 tgtcataatt attggcattt tgattattcc ctcttttatt cccttatttg tattctctat
     6841 agaaaaggag aatgaaacaa ctgaagatgc tatcaaatat gccatcgttc aaattgatgg
     6901 caaggaaatg gatcgcttcg atttggataa gatcaatcat aaactcgtga attatcatcc
     6961 tgcgaaggga caatataata ttgttgagat taaagatgga agaattcgag ttaaagaaga
     7021 taatagtcct gatcaaatcg cggttagaac tggatggatc tcaaaacccg gtcaaacgag
     7081 tatttgttta ccccataagt tagttattag tattgagaaa aaagaaagca aagattacta
     7141 catttactaa tctggaaata gatgaggagg tttttaataa gaaaaagata ttattattaa
     7201 taatagcagg ggctttatta agtgtgttaa gcgcttgtgg acaagaaaaa gaagcggtga
     7261 caacaggcac aagtatcttt aacgggacct gcaatggttt agttaacagt cggttagatt
     7321 acttatt
//
LOCUS       DAZUKT010000115         7042 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000115 DAZUKT010000000
VERSION     DAZUKT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.120"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="ACG816_002699"
     CDS             complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="ACG816_002699"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4921536.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="ACG816_002700"
     CDS             complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="ACG816_002700"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4921537.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="ACG816_002701"
     CDS             complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="ACG816_002701"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4921538.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="ACG816_002702"
     CDS             complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="ACG816_002702"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4921539.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3502..4482)
                     /locus_tag="ACG816_002703"
     CDS             complement(3502..4482)
                     /locus_tag="ACG816_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4921540.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4506..5990)
                     /gene="galT"
                     /locus_tag="ACG816_002704"
     CDS             complement(4506..5990)
                     /gene="galT"
                     /locus_tag="ACG816_002704"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4921541.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6002..6991)
                     /gene="galE"
                     /locus_tag="ACG816_002705"
     CDS             complement(6002..6991)
                     /gene="galE"
                     /locus_tag="ACG816_002705"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4921542.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1906 a         1547 c         1252 g         2337 t
ORIGIN      
        1 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
      181 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
      241 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
      301 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
      361 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
      421 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
      481 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
      541 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
      601 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
      661 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
      721 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
      781 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
      841 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
      901 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
      961 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1021 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1081 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1141 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     1201 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     1261 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     1321 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     1381 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     1441 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     1501 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     1561 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     1621 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     1681 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     1741 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     1801 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     1861 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     1921 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     1981 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2041 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2101 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     2161 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     2221 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     2281 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     2341 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     2401 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     2461 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     2521 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     2581 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     2641 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     2701 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     2761 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     2821 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     2881 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     2941 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3001 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3061 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3121 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     3181 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     3241 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     3301 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     3361 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     3421 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     3481 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     3541 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     3601 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     3661 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     3721 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     3781 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     3841 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     3901 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     3961 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4021 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4081 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     4141 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     4201 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     4261 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     4321 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     4381 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     4441 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     4501 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     4561 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     4621 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     4681 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     4741 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     4801 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     4861 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     4921 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     4981 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5041 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     5101 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     5161 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     5221 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     5281 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     5341 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     5401 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     5461 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     5521 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     5581 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     5641 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     5701 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     5761 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     5821 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     5881 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     5941 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6001 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6061 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     6121 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     6181 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     6241 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     6301 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     6361 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     6421 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     6481 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     6541 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     6601 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     6661 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     6721 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     6781 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     6841 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     6901 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     6961 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7021 tgtttgttga ttctatataa tt
//
LOCUS       DAZUKT010000116         6902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000116 DAZUKT010000000
VERSION     DAZUKT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.121"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..444
                     /locus_tag="ACG816_002706"
     CDS             <1..444
                     /locus_tag="ACG816_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS4921543.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="ACG816_002707"
     CDS             444..1643
                     /locus_tag="ACG816_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4921544.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="ACG816_002708"
     CDS             1659..2303
                     /locus_tag="ACG816_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921545.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="ACG816_002709"
     CDS             2314..3120
                     /locus_tag="ACG816_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921546.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="ACG816_002710"
     CDS             3142..3372
                     /locus_tag="ACG816_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921547.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3886..4194
                     /locus_tag="ACG816_002711"
     CDS             3886..4194
                     /locus_tag="ACG816_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921548.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            4196..4618
                     /locus_tag="ACG816_002712"
     CDS             4196..4618
                     /locus_tag="ACG816_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921549.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4638..6227
                     /gene="mobP2"
                     /locus_tag="ACG816_002713"
     CDS             4638..6227
                     /gene="mobP2"
                     /locus_tag="ACG816_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS4921550.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            6239..6574
                     /locus_tag="ACG816_002714"
     CDS             6239..6574
                     /locus_tag="ACG816_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921551.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6715..>6902
                     /locus_tag="ACG816_002715"
     CDS             6715..>6902
                     /locus_tag="ACG816_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921552.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2648 a         1036 c         1419 g         1799 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaatttata aagagcataa ggaaacgtgg gaacactttt taaaaaaagt
     3721 gttccaatag gctgtgccta ttacgtttct caaaaataat tgtgggaaca aaatatttaa
     3781 aaataggata gtgaaaggat agcaaaggat agtaaaagga tagaagcagg atagcgaaaa
     3841 ggataggctc cttttcgcta tcctttttaa attggaggaa ttaatatgga agtaagaata
     3901 agaaatgtag atgagaaaat cgtaagagaa attgatcgaa gatgtgacga actaagtaac
     3961 cgaacgggta ataaatggag tcgtaacgat tatttaaagc tactcgttga aaatgatttt
     4021 gatcgtcctt tgatggaata taaacaagat aaatttgatc aattattaaa tcagttaata
     4081 gataaatttt cttttgttca aagtcataat acaaaagtat tagaagaata tatttgtcag
     4141 aataatcaaa ttatcgaact tttaattgaa caaagtagag gaggagaagt ttaacatggc
     4201 caaagttata gacaagcata tttattttta tgaaacacat atagatgttc tagatgaaat
     4261 tattgcaact catgcagagg ttaaaaacta tagtgaagcc gttcggtttc tatgtatgaa
     4321 ttatgattca gaagtggaga aaaaagtaaa ttccaatcaa gcaaagttga atgcaatggg
     4381 aaaagaaatt tctattttaa ccgaaatggt ttcagagttt gcggatctcc atttgaaaga
     4441 tacagggatt ttaattggta caaattcaac tgtttaccaa gaagcaaaag agcgtgtgga
     4501 aggcaagata aaagcaaatc aaacgaagaa atattctcct aagataaaga aaacaataag
     4561 cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca aggttcttaa aacgataaaa
     4621 attagaagga gaatgtgatg agtaaatcac ctgcagtcat tctcacttgt caatttacct
     4681 tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa aaagcgctgg
     4741 aagaaaagga acgacgtacg cccgaggaag aacaggaact gcgtctaatc aagaatgctt
     4801 tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg ggtaaagaat
     4861 tcagcgaaac gtcgaatgaa gcgcaatcgt tgatgaaaca tggaatcaat tttgacaagt
     4921 tagaagaaca agattttacc aaatatcttt cttatatgac acgtcactat gcgttagaac
     4981 aaaagaaagt actgacgcat agtgaacaaa aagaatacca acgcttgaac caagcaataa
     5041 aaaaatatca aacagagcca cacaaaaaag cttcaggaaa tcttcctgga gttttttcta
     5101 tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caagcaaacc ttccaaagag
     5161 gacaacaaaa aggctcaatc atttatcaag atgttgtctc tcatgatcat tcgtatcttg
     5221 aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggt ttaaaagcag
     5281 ctggaagaaa aatgatggag acattgttcg agaaagagca actgaatgaa acaggctact
     5341 ggatggcaac gattcaccga aataccaaac atatccatat tcattttgca gttgttgaaa
     5401 aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat atcgtaccga
     5461 aagggaagag aaggcaagca acattagacg aaatgaagac taattatgta catgagttag
     5521 aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta ttgacacgga
     5581 aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt tatgatcgac
     5641 acgcaatgag cttattaact gaagtatata aaaacttacc taaaaagcgt tcggaatggc
     5701 attacgggga tgaaaatcga accaaacttt caccatacac aagagagaaa ctcgatgagt
     5761 taacccaata tgtgttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     5821 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
     5881 aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccattcta acagaactga
     5941 agaaacagga tagtcaaata aaacctttaa tagaaaggat ggatcagatg caatctattt
     6001 acaatgaacg atcctttcga acacttaaac agttgccaac gaatgaatac aaatatagtt
     6061 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg caacggagtt
     6121 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat
     6181 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat
     6241 gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga
     6301 acaacttccc gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgataaa
     6361 cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtactg
     6421 ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc
     6481 gccttattat actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag
     6541 cattgaagag tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa
     6601 gagtcctgaa cgtgattgtc caggactctt ttttttcact gaattgtttt ccgccaattc
     6661 attgagtggt gcgatttccg tctacattat atcaaaagaa aggaggaaga agaaatggct
     6721 tattcaacga ttggagttgt tgttgaaaaa agcagagaca atcttgtttt tgtgacagaa
     6781 attcaaacag gcagagcttt tgtagtcaca gacaaagcgg caaaagcata tcaaagtggc
     6841 gatattttaa cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcca
     6901 tt
//
LOCUS       DAZUKT010000117         6749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000117 DAZUKT010000000
VERSION     DAZUKT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.123"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(158..616)
                     /locus_tag="ACG816_002716"
     CDS             complement(158..616)
                     /locus_tag="ACG816_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4921553.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(629..1960)
                     /locus_tag="ACG816_002717"
     CDS             complement(629..1960)
                     /locus_tag="ACG816_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS4921554.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1971..3413)
                     /locus_tag="ACG816_002718"
     CDS             complement(1971..3413)
                     /locus_tag="ACG816_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4921555.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3400..4164)
                     /locus_tag="ACG816_002719"
     CDS             complement(3400..4164)
                     /locus_tag="ACG816_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4921556.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4268..5143)
                     /locus_tag="ACG816_002720"
     CDS             complement(4268..5143)
                     /locus_tag="ACG816_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4921557.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5398..5482)
                     /locus_tag="ACG816_002721"
     tRNA            complement(5398..5482)
                     /locus_tag="ACG816_002721"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5446..5448),aa:Leu,seq:caa)
     gene            complement(5500..5571)
                     /locus_tag="ACG816_002722"
     tRNA            complement(5500..5571)
                     /locus_tag="ACG816_002722"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5537..5539),aa:Gln,seq:ttg)
     gene            complement(5581..5653)
                     /locus_tag="ACG816_002723"
     tRNA            complement(5581..5653)
                     /locus_tag="ACG816_002723"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5618..5620),aa:His,seq:gtg)
     gene            complement(5715..5799)
                     /locus_tag="ACG816_002724"
     tRNA            complement(5715..5799)
                     /locus_tag="ACG816_002724"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5763..5765),aa:Leu,seq:caa)
     gene            complement(5820..5890)
                     /locus_tag="ACG816_002725"
     tRNA            complement(5820..5890)
                     /locus_tag="ACG816_002725"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5856..5858),aa:Cys,seq:gca)
     gene            complement(5936..6007)
                     /locus_tag="ACG816_002726"
     tRNA            complement(5936..6007)
                     /locus_tag="ACG816_002726"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5973..5975),aa:Gln,seq:ttg)
     gene            complement(6019..6091)
                     /locus_tag="ACG816_002727"
     tRNA            complement(6019..6091)
                     /locus_tag="ACG816_002727"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6056..6058),aa:His,seq:gtg)
     gene            complement(6094..6167)
                     /locus_tag="ACG816_002728"
     tRNA            complement(6094..6167)
                     /locus_tag="ACG816_002728"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6133..6135),aa:Trp,seq:cca)
     gene            complement(6174..6254)
                     /locus_tag="ACG816_002729"
     tRNA            complement(6174..6254)
                     /locus_tag="ACG816_002729"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6218..6220),aa:Tyr,seq:gta)
     gene            complement(6256..6331)
                     /locus_tag="ACG816_002730"
     tRNA            complement(6256..6331)
                     /locus_tag="ACG816_002730"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6296..6298),aa:Phe,seq:gaa)
     gene            complement(6367..6439)
                     /locus_tag="ACG816_002731"
     tRNA            complement(6367..6439)
                     /locus_tag="ACG816_002731"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6404..6406),aa:Asp,seq:gtc)
     gene            complement(6449..6521)
                     /locus_tag="ACG816_002732"
     tRNA            complement(6449..6521)
                     /locus_tag="ACG816_002732"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6486..6488),aa:Val,seq:tac)
     gene            complement(6537..6608)
                     /locus_tag="ACG816_002733"
     tRNA            complement(6537..6608)
                     /locus_tag="ACG816_002733"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6573..6575),aa:Glu,seq:ttc)
     gene            complement(6623..6712)
                     /locus_tag="ACG816_002734"
     tRNA            complement(6623..6712)
                     /locus_tag="ACG816_002734"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6676..6678),aa:Ser,seq:gga)
BASE COUNT         2045 a         1482 c         1094 g         2128 t
ORIGIN      
        1 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
       61 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
      121 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
      181 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
      241 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
      301 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
      361 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
      421 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
      481 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
      541 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
      601 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
      661 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
      721 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
      781 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
      841 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
      901 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
      961 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
     1021 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
     1081 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     1141 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     1201 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     1261 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     1321 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     1381 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     1441 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     1501 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     1561 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     1621 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     1681 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
     1741 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     1801 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     1861 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     1921 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     1981 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     2041 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     2101 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     2161 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     2221 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     2281 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     2341 aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     2401 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     2461 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     2521 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     2581 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     2641 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     2701 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     2761 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     2821 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     2881 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     2941 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     3001 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     3061 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     3121 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     3181 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     3241 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     3301 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     3361 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     3421 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     3481 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     3541 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     3601 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     3661 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     3721 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     3781 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     3841 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     3901 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     3961 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     4021 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     4081 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     4141 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     4201 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     4261 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     4321 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     4381 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     4441 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     4501 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     4561 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     4621 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     4681 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     4741 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     4801 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     4861 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     4921 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     4981 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     5041 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     5101 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     5161 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     5221 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     5281 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     5341 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
     5401 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
     5461 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
     5521 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaag
     5581 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
     5641 tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa
     5701 gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga
     5761 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa
     5821 ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg
     5881 ctataccgcc attatactat tatcaatcta taaaataaag aataaattat agaaactggg
     5941 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
     6001 taccccaaga ataaaaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa
     6061 tccggtgcgt taaccacttc gccacattcg ccatggcagg ggcagcagga attgaaccca
     6121 cactaacggt tttggagacc gctgttctac ctttaaacta tgcccctata aaatggagga
     6181 gagtggattc gaaccactga accctaagga acggatttac agtccgtcgc gtttagccac
     6241 ttcgctactc ctccatggtg gcgcgggaca gaatcgaact gccgacacac ggagcttcaa
     6301 tccgttgctc taccaactga gctaccgcgc catattttaa aattattaat tgaataacaa
     6361 gaacgacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta
     6421 acccctacac cacggaacca gttcattatg gaggttaacg ggatcgaacc gctgaccctc
     6481 tgcttgtaag gcagatgctc tcccagctga gctaaacctc caacattcaa ttaaaatgac
     6541 ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc
     6601 aacgggccag gtaaatttgc tacggagagt aagggattcg aacccttgag acagtttggc
     6661 accatctaca tgatttccaa tcatgctcct tcggcctctc ggacaactct ccagagtgaa
     6721 agaagctcaa tactctttct caaaatgag
//
LOCUS       DAZUKT010000118         6642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000118 DAZUKT010000000
VERSION     DAZUKT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.124"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG816_002735"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG816_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG816_002736"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG816_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS4921558.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG816_002737"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG816_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG816_002738"
     CDS             complement(2143..2373)
                     /locus_tag="ACG816_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS4921559.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG816_002739"
     CDS             complement(2603..3421)
                     /locus_tag="ACG816_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4921560.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG816_002740"
     CDS             3582..4271
                     /locus_tag="ACG816_002740"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4921561.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG816_002741"
     CDS             4285..5787
                     /locus_tag="ACG816_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4921562.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG816_002742"
     CDS             5800..6276
                     /locus_tag="ACG816_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4921563.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG816_002743"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG816_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag at
//
LOCUS       DAZUKT010000119         6517 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000119 DAZUKT010000000
VERSION     DAZUKT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.125"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..826)
                     /locus_tag="ACG816_002744"
                     /pseudo
     CDS             complement(53..826)
                     /locus_tag="ACG816_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            862..2049
                     /locus_tag="ACG816_002745"
     CDS             862..2049
                     /locus_tag="ACG816_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFS4921564.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            2782..3750
                     /locus_tag="ACG816_002746"
     CDS             2782..3750
                     /locus_tag="ACG816_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4921565.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3818..4576
                     /locus_tag="ACG816_002747"
     CDS             3818..4576
                     /locus_tag="ACG816_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS4921566.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKKIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4644..5354
                     /locus_tag="ACG816_002748"
     CDS             4644..5354
                     /locus_tag="ACG816_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4921567.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            5390..>6517
                     /locus_tag="ACG816_002749"
     CDS             5390..>6517
                     /locus_tag="ACG816_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4921568.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2196 a         1001 c         1329 g         1991 t
ORIGIN      
        1 gccaataaaa cttagtccga tatgcggcgc gttctgtgcg ccgcatattt ttttagtttt
       61 caaagatcag accttgttgt aaatagatcc gtgcccgaaa gttataaaag ttgcgataac
      121 caaacgcaat tcgtttaatc agcttgatct tgttatacag cccctcagtg acgccgtttg
      181 agtgggacac cttaaaggca tttgaaatac ccgattggta gcgatggaag atcgtgaatt
      241 tctttttaaa cgactgaggt aacgtagatg aaattgtttt aagggtttct tgaaagccta
      301 ccgtatctcg tttaacgaaa taatagcgta ataattgaca aaaatgatac gcagttcgca
      361 attcttcgtt gtagcttaac agctcattaa cgatttctgt ttgactaatt ggtcgtttaa
      421 acagtcgatg ataatgccgt ttgagtgcct cccatgtcgt ggaatctttg agtaagagtt
      481 tccaatatcg cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac
      541 gattcatgat ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa
      601 agcggtcagt tacgatctcc gcatttggaa agaccgtttt aagcaactga caatagctgg
      661 cattcatgtc catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga
      721 agtattgagt tagtttgtaa agtctgcgat cttcaagggt cgtaaatagc tgattggttg
      781 tcccatcaac acaaataaaa ctcatggaac cagaagtggc aacattgtgt acttttcttt
      841 gcaatcttgt gtacaaaact attgcaaaac acagcgcata agaatcctta caaaacagta
      901 gatgaagcaa cccaattaga tcttgttata gttgttaatc agctcttaac gggttacgat
      961 tcaaaatggg tcaacaccct atacttagat aagtcacttg aatatcagaa cataattcag
     1021 gctttttcac gcacaaatcg tcttaatggt cctgaaaaac cgttcggaac cattcgttat
     1081 tatcgtaaag cacatacgat ggaacagaac attgaagagg ctattgagtt atactcaggc
     1141 aaacaagcaa aagggttatt tgttgaaaaa ttatataata acttagaaaa gttgaacgat
     1201 atattcgaag aaattaagtc cgtatttaat atttctggca tagaaaactt cagtcattta
     1261 ccggaggttc caggggaaat taagcaattt gtaaatcttt ataatcaact aatccattgt
     1321 tatgaatcag cgatgataca gggattcaca tgggcaaaat cgacttatga aatccaggat
     1381 tatgaaccta taacagtagt aatcacagaa tatgaattta atatcttggc acaacgttac
     1441 agcgagatag agagagaacc tagagacgga aatgatagta tagcgtatga cattgattac
     1501 caattacctg aaggtagtcg tagtcgtatt aattatgagt atttaaacac aaacttcaaa
     1561 aaatacatga atataaaaga agaacgtgat tcgaatttaa ttcaatcatt gttaaacgac
     1621 ttacatcgca catttgcgca attaccagtg gagttacagc catatgctga gatgattcta
     1681 aatgatgtaa ataatgggga attagttatc aaagaaggat gggaatttac tgattacctc
     1741 aatgaatatc agcttagtga agaggacgat tttatccaag atttagtgga ttcaaccaat
     1801 attaatgagg cattattacg tgagatgttg gatttacgct taaccgaggt aaacattaat
     1861 gaatatagtc gatttgataa attaaaatca agtgttaacg taggtcattt cagtggatat
     1921 atcgagaaaa atttaggtaa aatggtaata ccaatcaagg taaatatgct aattgacaga
     1981 ttattgcgtg catttttatt agaagatgta ttcgatgtta cagagtacat aaagaattac
     2041 tttaactaga gtacacctac atcttatagt acataaaaaa attcatcttt tttagtttaa
     2101 taattcacca aaaaggtgag taatgccatt tttgaaaatt gagcttgttt tatgtctaca
     2161 tctgtatccg tatataatcc aaaggattat aaactcagaa gagtctttat tttttctgaa
     2221 tataactact gtatcaaaaa caatcgatta tgagacaaaa caaaatgacc tcttttattt
     2281 aagaaaggag gtcatttttt gtatcataaa ttgtattaaa atcaatgtat cactttttaa
     2341 tcaattttaa gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt
     2401 ttaagccatt ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact
     2461 ccagtagttt cctaattcaa ctgtgtgcaa aacgatttct tctaaacaaa tcaagacaaa
     2521 cttataaacc ttcatcccag caccataatt atttaaatca taggcatttt tcgaaattag
     2581 tatatgtact caagtttcac tgcagattat ttatattttt tagagaaata aatttttcct
     2641 attgacaaac acaaagaaag cggatacaat acaattatgt cacatgacgt tatatgacgt
     2701 tatgtaatat ctttacattt aatcagttac ttttattttg gataaaatta acatttttaa
     2761 aattttaagg aggaaatgtt gatggatgta gcaaaaacgg ttaacgcaat tgtagaaaaa
     2821 aatgatggag agatttctgg aatttatttg gtggcttgtg gcggttcgct ggtagatatg
     2881 tatgtctcag attattttct taaagccgaa gcatcgaagt gtattacagg cttatataca
     2941 gcgaacgaat ttaaccatgt aaccccaaag caattagatg ataaatctgt tgtcattcta
     3001 tgttctcatg gaggaaatac agcagaaacg gtaaaagcag gagaagtcgc tcaagctaga
     3061 ggagctgtga ctgttggtct gactcataat aaaactgcgg atttattaaa ggtttctgat
     3121 catagtttcc tttacgaatg gggagatgat gcaaaaatag tcaataatcc tatggccatc
     3181 attcttgatt taactgtatc tttggtcaat gttaccgaaa catttgaaca ttacggtaaa
     3241 ttcagagaag gcatgaaggt tatcgatacc gtaattgaaa atggcattaa gcaggtgcag
     3301 gatcgtatta aagtgtttgc tgataagtac aaagatgaaa caatgtttta tatcttaggt
     3361 agtggaccat catatggaca tgcttatggt ttttcaattt gctctttgat ggaaatgcaa
     3421 tggcttgatt caacttctgt tcattctgga gaattcttcc atggaccttt tgaagtaaca
     3481 gacaaagata cgaattttat tctcttgatg agcattggac gcactcgtgc tttagatgaa
     3541 cgggcaaaac gattccttga taaatatgca gaaagagtag aaatcgttga tgcaaaggaa
     3601 ctaggattgg atatgatatc agatgaagtt tctgaattct tcaatcccat tttattctac
     3661 agtattcttt cagaatatcg ttcagcttta gcagatgcac gcaatcatga tttagatgtt
     3721 cgtcgctata tggggaaagt ggagtactag gaggattaaa atgaaggtat taggcttagg
     3781 tgataacgtc gtcgataaat atgaaaatct gggaattatg tatcctggag gaaattcatt
     3841 gaattttgca gtttttgcaa aaaaactggg acatgaaagt agctttatgg gagtttttgg
     3901 ctcagatgag gaggcagctc atgttttatc agcaataaaa aaaataggac tggacaacag
     3961 tcactctcgc tttgaaaaag gcgagaatgg ctgtgccaga gtgaagattg atgagggcga
     4021 ccggattttc cttggcagca attcgggagg tgttactcgt gaatacccga ttcagcttac
     4081 tgaagaagac agagaatatt taaaccagtt tgaattgatt catatgggat tatatagcca
     4141 tgtcaatcat ctattagaag agttacaaaa tgtttctgga aagatttctt atgatttttc
     4201 tgacgacttt acggaagaag aggttcagcg ggcaattgat aaagttgatt ttggcttctt
     4261 ttctatagaa gacttttccg atgaagaagt aaagcagttt ttgatgaagc atttttgtga
     4321 gcgaaatgat gtattgattg caacccgagg agaaaaaacg gctattggtt atgatggagc
     4381 aaagttttat gaagtgaacc ccgtgctaga agaaccagtt gatacaatgg cagcgggaga
     4441 ttctttctta acagctttcc tactttattt tctcgaaggg gacgatatag tcaccgcgat
     4501 gaaaaagggg aatgaatttg ctgggaagtc ttgtctggtt gacggttcgt tcggctttgg
     4561 tattcattac gattgagctt gttttaaatt cctgcatttg ataacctaaa gaaagaaatc
     4621 ccggtaaagt ggaggttaaa aaaatgctgg atacaaatgc acaattacca ttgtacttac
     4681 aattaaaaga aatattaaaa aataaaatca atacagggga gtatcccgag aatacgaaaa
     4741 ttcccgctga atcagagctg atagaaagtt atgaggtcag ccgaatcaca gtgagaaaag
     4801 cagtagaaga actggtagaa gaagggtacc tacttaaaaa acaagggaaa ggaacttttg
     4861 tcaaccggca taaagttttt cgtaaaattg aatatgttat gggtttcagt gagagttgta
     4921 aatctaatgg ttttacaccg actagcattg ttttggaacg caagatcatt ccagcaactg
     4981 aagggatggc aaaaaaactc gatatcgctc aaggtgatga tgttttatat atccagagaa
     5041 agcgaatggc ggataacatc cccattatgt tagaaaacaa ttatttttct tatgaaagat
     5101 atagtttctt aatggaggaa gagctgactg attctttgta tgaagtactt aaagtccaag
     5161 gtaccttagc catcaatcct ggggaaactg cacttgaaat tgtcacagct gatgaccaaa
     5221 ttgccaaaat aatggaagta gctgttggga cgcccttttt ctttatggat actttaatca
     5281 acgatcaaaa caacatacca attcatgtag gtaatcaata ttacctggga gaagtatatc
     5341 gtttttactt atgatttcct gttttgattt caaggagtag attttattta tgaagcaagt
     5401 attgcggtca aaaatgattt ttgatggaga gcagccacct ttcaaaggtt atattgtaat
     5461 caatgaaggg atcattgaaa aggtggaaaa caactggaat tacagtgatc tattaaatga
     5521 aaacacggaa ttaattgctt atgatgatca attcgttatg ccagcaattc atgataatca
     5581 tgtatttttc agtggttatt tggctatgaa tgctggcata gatttaagcg atacatcttc
     5641 agctaacgag gctgtagagc gtattaaagc tgtaatgaac cataaaaaag cagatgaacc
     5701 aatttatgct catggctggg atgctgccct gtggaaaact gacccatcag aagagctatt
     5761 gaatgaaata aaccactttg gtccaatcac agcgatcgac aaaaatcgca gccattgttg
     5821 gatgaatgct attgctaagc agacttatgg ttttacagaa aaagaaacca gtgctgaaag
     5881 tcgagtatta ttaattaagg aaatgctagc aaacaaaaag cttttagcag aggtttacgc
     5941 tgaatttgaa gcattactgc tttcaaaagg tgttatatca atgaaagaaa tcgtttttga
     6001 tgacgcagaa tttctttcta atatacctca gaggaaaata cttagtaacc tatatgtgca
     6061 ggcagtccaa gaacctctac agcatgaact tctatatgag tataaggaaa aagttttccc
     6121 tgaaaaggtc cgctttggtg gagtgaagat catggtagat ggtgttgttg cggatagaag
     6181 tggagatatt tacggtgttt atcagtcagg agtagcttca cccgagattg actatgaggc
     6241 aattaaagca gaagttgctg cattcaatga agtagagatt ccttgctgtt tgacaacgga
     6301 aggagataaa gcgggttata aagctgcgtc aattcttgtc gaatttggaa agcggttacc
     6361 tgaaggcgtt tttaattcga ttagtgattt ggaaatggtt actcaaaaga cagcagaaaa
     6421 aatgaacgaa ggacaagtag tagcagaggt atatccacaa attctcggct tgaatcaaac
     6481 gttagaagag gcttacatgt caactgttat aaaaaat
//
LOCUS       DAZUKT010000120         6494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000120 DAZUKT010000000
VERSION     DAZUKT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.126"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG816_002750"
     CDS             complement(99..1229)
                     /locus_tag="ACG816_002750"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4921569.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG816_002751"
     CDS             complement(1878..3080)
                     /locus_tag="ACG816_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4921570.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG816_002752"
     CDS             complement(3140..3343)
                     /locus_tag="ACG816_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4921571.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG816_002753"
     CDS             complement(3485..4552)
                     /locus_tag="ACG816_002753"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4921572.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG816_002754"
     CDS             complement(4610..4717)
                     /locus_tag="ACG816_002754"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921573.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG816_002755"
     CDS             4880..5692
                     /locus_tag="ACG816_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921574.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG816_002756"
     CDS             complement(6066..6257)
                     /locus_tag="ACG816_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921575.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1961 a         1320 c         1015 g         2198 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggt
//
LOCUS       DAZUKT010000121         6488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000121 DAZUKT010000000
VERSION     DAZUKT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.127"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            215..553
                     /locus_tag="ACG816_002757"
     CDS             215..553
                     /locus_tag="ACG816_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921576.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            639..884
                     /locus_tag="ACG816_002758"
     CDS             639..884
                     /locus_tag="ACG816_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921577.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            904..3060
                     /locus_tag="ACG816_002759"
     CDS             904..3060
                     /locus_tag="ACG816_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921578.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            3072..3305
                     /locus_tag="ACG816_002760"
     CDS             3072..3305
                     /locus_tag="ACG816_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4921579.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3295..4161
                     /locus_tag="ACG816_002761"
     CDS             3295..4161
                     /locus_tag="ACG816_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921580.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4176..6146
                     /locus_tag="ACG816_002762"
     CDS             4176..6146
                     /locus_tag="ACG816_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921581.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         2502 a          887 c         1259 g         1840 t
ORIGIN      
        1 aaaacttgat aaaccagcat ttttagccaa taaaaaaagt gaaataattt aatggaaaat
       61 ctcttttaat catcgaaaat ttatgttatt atgataacat aaagatgtaa atatcgagac
      121 gtctttttct tagttagatc attgacaagt gaacaaataa gatttgtcga tgatataatt
      181 aacttattaa aaatattaaa aaggaggttt ttatatgttc gattctatta aaaatttggt
      241 tagcggaatg acgcctgata tgaaaacttt agtacaagtt gttttgtatt tcttggcatt
      301 cgttttttcg ttgaaaccag gattcactgc aatgaaattt tttggtgatg caaaatgggg
      361 agatggatta aaatatgttg gggcgattat tgcaatcttt attattcctg taattacaac
      421 cgttatgctt tacacaatgg gtactaaaac aggtaatgat ttgaaccaaa aagctaatat
      481 catatcagca cttattccag ttgttggtat ctatctagtt tcaaaatttc caaaaaatga
      541 actgttaggt taaaagtgaa ttgaacaaat ttggataata tgccgatttg ttcaatttac
      601 tttttaccaa aaaactacgc tccaagcgta gtttagaagt gagggaagaa agtaaatgta
      661 agatggtttt tgatagagaa aaaatgttag ctcacgccaa cgaagtatta atgagcagtc
      721 tgaaaggtac cgaactggca aagataatga atatgaatgt taaccagttc tatgattatc
      781 gaaatgggag taaaaagatt gaaaaagcca gacttgaaac gcttattaaa ttcgaaaaag
      841 catatgtata tatgctagat aaacagaaaa gaactattga ctgaaaaaag gagttttaca
      901 ataatggcaa aattcaaaga tattaccgaa tctattaatt atgtggcaga aacagttcct
      961 gtagttgcac tctctttgaa gccacaatta ctcgaagtgt acaaaaaaga agaaaaagaa
     1021 gcgaaggcaa aaaaagctaa agcaaaggta tggaaagaat tcgatgcgtt gctgaccaaa
     1081 gatttaccaa agaaaaatta tatggtaatt gaagatgtac aaaagctcaa cagtgaggga
     1141 gttatagatg aggatgatgc tattctcgat tctgagaagt atgtcgttct attgaagtca
     1201 agtttgaatt atgtagctga tctaccagaa aaatttaaag agaagctatt agcaggtgtc
     1261 cgttttatgg atagtagtgg tcaattatta gttattccgg atgatcctaa tgtgtttgaa
     1321 tcagaagttg aagaagctac agacttctat agcgcagtag agcaattaga agtagctact
     1381 aatggattag aacgaattgc tgagaccttc aactcttctg aagaagttga atttgaagcc
     1441 gtagcagaaa atgtaccaga tgaaaaagaa gctctttttg tggatgataa caaaccaacc
     1501 gaagcagaaa accaagaagt aagcatagaa gaaacacaaa tacaaacaga aacctaccaa
     1561 caaccaaaaa ttgatgaaaa acccgaatcc caaagtgaca aagaaaatta tgtttatgaa
     1621 gctgaaaaaa tcacatctga aagtgatgta ccgaaaaact tagatgctga ttttttagaa
     1681 aaaaattttg aacagatcat tgctgacaaa tttcaacctg tagttttaga tgagattaat
     1741 gaccctgtcg atttaaacga ggagatgcta tccatacctg agttgaaacc attgctgttt
     1801 ttagctaaag agagtatgaa cgctcgcatc tctattgcta ataataaaat caacagtgaa
     1861 ttaaataaga cgaaggagca attgatcctc aaaggaaagc aaatgttagc tgatgagctt
     1921 ttgcggatag aggaagcaac ctcattaaat ggtcaaaatc agtttacaaa tacactatcg
     1981 gttgctaaag agagctacga agcagaagca tctaaaattg atgatttagt gatggaaaaa
     2041 tccaatatca ttcgtaaaga ttatgatatg gcgaaacagg cttatttgga tgctgaacta
     2101 aagaaactag agcaacggta tgatgctgag catttaccag aagttaaaca acaagaagat
     2161 gaatatagaa aagcaactga agaaagtctt gatcaacagt accaagaagc aattgaatct
     2221 attaaaaacg atgcattgaa acaaagccaa gaagcaaaag aagttgtggt ggaacgaatt
     2281 atcaattcat tgaaaccaga aattgaaaaa tcattaacag acgtgcaaga aaaaacttta
     2341 gagattgtcg aaaaaacaaa gtcagaaaat gaagctgagt tccatgatac aaaagagaaa
     2401 acaacagatg tggttgtcaa atattttgcg aacaaagaag aagttgaaaa gcaggtagct
     2461 aacaacacga aagaattaga aacaaaactt gtagagcaac aaaaaactat cgaagaatat
     2521 aaagatcgaa tgaaagaggt caaagattta gcagagggtt ataaattaaa tagcgaaaag
     2581 ttggcagaac aaagtgaagc tttaagagaa gacttatctc gttcccgaag tgatctagct
     2641 caaatggcag aaaaaacgaa atcattagca gttgctacag aaaatcaatc aagttcaaaa
     2701 cgtgatttta caagtaaaaa gatggctact atcatttctg tcgctgcaat ggcttgcgga
     2761 acgatcttgg gatcgtccta ctttattagt cataataatc aacaagcaca ggctcaaact
     2821 caaacatcta tttcgagagt ggaaaattcg attgggacaa atacgaactc tgcttcaaca
     2881 aatagtagct atgaagcaac accaactaca gagttcaaag ttgggaaaga tgttgtcatc
     2941 agtattgatg ggaaaacaac taaggctgaa gtgatttcta ttgatggaga aaaagcagtt
     3001 gtgaaaactc cagacggaaa tcagtacaaa attcaagctc acttatctga taataactaa
     3061 ggagggctat tatgcgagaa gaaaaaatag tttatccagt aattcaaaac aatcaagaaa
     3121 acatgacctc ttctcagcac ttacaggaag cacagctcgt gtatcaggta aatgaactac
     3181 cacaaacgaa cgagacaatg agcaagtcaa tatctttaat tggcttgctt attgttgcaa
     3241 tagtgataac tgtaggtgtt taccgtaagt tgaacgtgaa aggaagtatt tctgatggca
     3301 gataactcaa actatggatt taaacataac ttacatcaaa aaccttctga aacaattgtc
     3361 agagttgaat ggcttgaaaa gcatgtcaat agcacctatt ctgttacagc aaaaagatta
     3421 attgtatcag ctatagttgt tgcagtcgtt gttcttggat caattgttta cttagtttct
     3481 gatacgatta aactgaaatt tatttttgtt ccaacgttat taatgataat tggatttctt
     3541 attctaatta gccagtatat gtttattgat ccatctagtg gttcattgat ttctggttct
     3601 attagactgt tcaaatcagt agttaattca gcaagagtat ctatggggaa aaagaaacca
     3661 tttcgaggca cagagatttt aaaagtgaaa caagatggaa caatcgtata tcgagatggg
     3721 agtgtagcat taatgttcaa tctagatggg gctacatcac taaccgcttt tccagaagaa
     3781 gttatggctc aggaatcgaa atcaatacaa tatcaaaata ccagagatcg ttccttagct
     3841 gaaatcaaaa tcacttcatc tcaacctcag aatacggaaa gacaaactcg aaatatgaaa
     3901 atacaaaaac gaaatgcaag gagtaacaaa gcagtctttg acataataga tcagcaagaa
     3961 cgatttgtat cagaatatat tgatggtgtg aaaacaacag taatccaata tcttatcttg
     4021 aaatctcaat ctaagatggt attggatgaa ggtgtggaac gtttagaaaa ctatacgagt
     4081 agaggacttt attatagtgt cactcagttg tcagctaaac aaacaaaaga aatattatct
     4141 gatatttatg cactgaaata gaggtgagag attaaatgga aattatgggt agatcacaac
     4201 tattaaattt caaaaaaaag agaaaaacta aaaaagaaca aaaaactaga aattcaaagg
     4261 tagtgaataa tacctttgtt agtgatttaa catctttagt tggttatcaa tttacaccat
     4321 cttacattag gacaggtaag cggtggggaa caattgtgaa attagtgaat acttatggaa
     4381 tgaatagaaa ttatggattt ggttggatgg ttaaagctat tcctgaagtg agagtagaag
     4441 gggtaaaagc ctatctaatt gaagcagata agccagtgtc acaaaaagat caacaagaaa
     4501 taatgcacga gaatgttggg ggatcaacga atagttatga aaataattta gaaggaaaaa
     4561 attcagatgc tgaaattagc aaaactgtta tgcggattat ggatttgaat acagcatctt
     4621 tactagacag catggcaaat aaaattattg attggaagct agaaatccta ttagtatccg
     4681 attctcctaa taaaatcagc gaacagctaa ttaaactcca acggttgtat gatgaaaaaa
     4741 tgtatggatt aaagcttatg tcatatggag gagatcaacg tgatctgttt gatagacttt
     4801 taagtgaacc aatcgggaat gtctatgagt atacgtctat gagttcggat tttgcaggaa
     4861 atgatcatgc aattcgcaaa ggtttagatg atgcgaatgg atgggctatc ggcactatgt
     4921 ctactagcta cgcaagtggt gtagctttta tggcactgga tgattctttt acaagtaaag
     4981 ttttagtggc atcacacaaa aacgctacaa ttaaaggcta tgatgaaaaa atctcagcat
     5041 catcactttg ggggcaattg attgctaata atgcaatggc aaatcataaa cgaacttttc
     5101 atattgtctt aaataaattt gattactttg gtgatccaac tggtagaaca tttgttgcca
     5161 aaccttcgtt aaacagtgta atagaaaagg ttaatttggc tgaagaaggt cttaactctt
     5221 tggagctttt tggtaatcaa aaggacattg gtgatttata tagacggaat ctcgataaac
     5281 taactcagat tttttatttg ttagcaggca gaacattaga ttctgcaact agagttttat
     5341 tgaaaaaaga attaggtaac ttttatataa gtcagaaact ttgggatatt aattatgaag
     5401 aacgttcaaa acttttggtg aacagtagtg atacaatgcc agttcttggg gattttatta
     5461 gctatttgac taatgctgaa acaaaagcca aaaaatctgg tactgaaaaa gaagtcgatc
     5521 gtattattgt attaaaagac acattgagaa cagcattaga cagcttctct aggatgttta
     5581 atcaaagaac gacactgaaa gaaatcggta aaagtgacaa attacagttt tactataact
     5641 taggtgatct aagagcagaa accaatgtta tggaagctca atttttaaat acctttgatt
     5701 tcatcacatc acaagcaaaa aaagatgata ttgttatgat tcacggcttc gataagttat
     5761 ctattgaaac cgttgggcta attaaaaaaa gattaacaat gcttgaagaa gatggaattc
     5821 gtcttgcctt tctatttgat acaatcggtt caggagaaga caatgaatac aaagaagata
     5881 ttgaaacagc tgatatattc aatacagatg gaaaaattta ccaaagtttt gaaagtgatt
     5941 ttaattacac tatcattgga aagatgacca gtggagagtt aatgaagtat gaaaaaaaag
     6001 ttgctcctgc tagaaaagga gaagatgatc aaaaactttc tgttgatttg agacagtatt
     6061 tgacaacagg aagaagtaat aaatttcaga tcaggagacc agtggatctg actaataatt
     6121 ttgttattgg agagtttatt ctttaagttt tatctttgat tttgagtgta taaggaaagg
     6181 ataagaaaaa acatgtatat ttgagactaa aaacagtctt agagaacatg gtacgttttg
     6241 tgaaaagaga actatagaaa tttcataaag tgaaacctta ctgagtgaaa taattatagt
     6301 ggaggttaaa taatgaaacc aaacataaaa attttagatc gaatatttct aggtagagac
     6361 acagaagtaa tcctaattca acatgaagaa ggatttgaag ttagtatagg tattcagaaa
     6421 cttcaaaaac ctcattattg taatcagttg tataaaaact ttactgatga agaaaaagcg
     6481 agagtttt
//
LOCUS       DAZUKT010000122         6317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000122 DAZUKT010000000
VERSION     DAZUKT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.128"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG816_002763"
     CDS             330..1175
                     /locus_tag="ACG816_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4921582.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG816_002764"
     CDS             1456..2676
                     /locus_tag="ACG816_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4921583.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG816_002765"
     CDS             2673..5393
                     /locus_tag="ACG816_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4921584.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..>6317
                     /locus_tag="ACG816_002766"
     CDS             5377..>6317
                     /locus_tag="ACG816_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HFS4921585.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKD"
BASE COUNT         2354 a          977 c         1224 g         1762 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagattt
//
LOCUS       DAZUKT010000123         6281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000123 DAZUKT010000000
VERSION     DAZUKT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.129"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG816_002767"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG816_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4921586.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACG816_002768"
     CDS             499..861
                     /locus_tag="ACG816_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4921587.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACG816_002769"
     CDS             913..2595
                     /locus_tag="ACG816_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4921588.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACG816_002770"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACG816_002770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4921589.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACG816_002771"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACG816_002771"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4921590.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACG816_002772"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACG816_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4921591.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2088 a         1093 c         1431 g         1669 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta t
//
LOCUS       DAZUKT010000124         6230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000124 DAZUKT010000000
VERSION     DAZUKT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.130"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACG816_002773"
     CDS             133..633
                     /locus_tag="ACG816_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4921592.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACG816_002774"
     CDS             730..2577
                     /locus_tag="ACG816_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4921593.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACG816_002775"
     CDS             2580..3476
                     /locus_tag="ACG816_002775"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4921594.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACG816_002776"
     CDS             3526..3915
                     /locus_tag="ACG816_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4921595.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="ACG816_002777"
     CDS             3902..>6230
                     /locus_tag="ACG816_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4921596.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLN
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          926 c         1335 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 ctttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAZUKT010000125         6191 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000125 DAZUKT010000000
VERSION     DAZUKT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.131"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..680)
                     /locus_tag="ACG816_002778"
     CDS             complement(3..680)
                     /locus_tag="ACG816_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4921597.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(798..1373)
                     /locus_tag="ACG816_002779"
     CDS             complement(798..1373)
                     /locus_tag="ACG816_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4921598.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            1504..2208
                     /locus_tag="ACG816_002780"
     CDS             1504..2208
                     /locus_tag="ACG816_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4921599.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            2186..2983
                     /locus_tag="ACG816_002781"
     CDS             2186..2983
                     /locus_tag="ACG816_002781"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4921600.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            2985..3884
                     /locus_tag="ACG816_002782"
     CDS             2985..3884
                     /locus_tag="ACG816_002782"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4921601.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            3902..5263
                     /gene="mgtE"
                     /locus_tag="ACG816_002783"
     CDS             3902..5263
                     /gene="mgtE"
                     /locus_tag="ACG816_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4921602.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(5325..5966)
                     /locus_tag="ACG816_002784"
     CDS             complement(5325..5966)
                     /locus_tag="ACG816_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4921603.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            6075..>6191
                     /locus_tag="ACG816_002785"
     CDS             6075..>6191
                     /locus_tag="ACG816_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HFS4921604.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKN"
BASE COUNT         1867 a         1132 c         1345 g         1847 t
ORIGIN      
        1 ttttaattag aaagcaagtg taaatggcta gttcttgaat ggaactgaga atcatttgta
       61 tgtaaatgtt caccattaaa aacttgatat gagttatctg ttgttttaca cagctctgtg
      121 attctccaaa ggctacttgt accttttact tctactaaaa caccaaaatc acggtcgcag
      181 gcataattgt aaacgactgc agaagggtgt tttgtgatgc ccatttcccg agcaattttt
      241 tgatcagaaa agaatacttc tttaacgaag tcagactgac gatcttcttt aaacatattc
      301 aaatcgccgc cgatttcttc aaatatggat tcagcaagtt cttgagaata tggtatgtca
      361 ttacagccga ctgcttcttg caatttgatc aaaaaacggc ggcctttttt ctttccttga
      421 aactgcgccg ctttgcagtc caatgctgca gaataagtca ttgagaataa gtgattacgt
      481 tcctcaagat tgttctgaga aattcctttt tgttgcatga catccccaat tgtctgcaaa
      541 ttgaccaaag gaagaaagcg aaattgcatc ttttcttttg atggtgtccc gtactcttct
      601 tcgataaatt gtaataactg ttgttccatt tcgaaacagt attcccctaa aggattaaca
      661 aataaataga tttcgatcat atgaatctcc cccaaaatag attctataca actagtgact
      721 agattctttc acatcctata acttaacaga aaaaagtaaa aaaatgaact caagtgcctt
      781 gtgaaaatga aaggtttcta atttttagct tcaagcatac gttggatctt gttcttagca
      841 ggacgataag gtaaagaaaa acgttttaaa aaatttataa aagcaatttt tcccacagat
      901 gcatccccta cctctagttc cagttcaaaa tcgtgtagat tgtctcccca gctttcatca
      961 atagctaaaa gtccttcttc aatagggaat tcagctcgtt ttgttgtaag ctttccggtt
     1021 tggattaatg aagcaggatc gatattcaaa tcagaaagtt tttgaaaaac tgcgcctgaa
     1081 cgaggcagac gtttttcgaa gataaatgct tctgcttctt ctgttataaa ggtatccgtt
     1141 gtttcgagac gccctttttc agcaggtgtt tttaatgtat attcagcttg atgaccaacg
     1201 aaacggatcc gtaggccaca attttgtttc ttcagctgtt catcaacggt atcgaaataa
     1261 atattagtct gtacacggaa atctttatct gttaattgat agtgagcgat gaatatctcg
     1321 tactcttgct ttgtcagcat agatttatat tcgatttcaa gcatttgtgt catttgatcg
     1381 actccttttt gtctattttg cctaattttc tttttttggt caatgcataa atcaagagta
     1441 cgaaaaaact ttgcatatat ggtagaataa acaatgaatg ttaatgaagt agaggtatca
     1501 gctatggaac gagattggga agcattttta gcaccatatg aacaaactgt atcagaattg
     1561 aaagtgaaac tgcgggggat ccgcaaacag ttccgtgaac aaaatcgaca tgtcccgatt
     1621 gaatttgtca ctgggagagt caagccagtc gatagtattg taacaaaatc acaattacgg
     1681 catattccaa tgggacgttt agaagaagaa atgtctgata ttgccgggtt aaggatcatg
     1741 tgtcagtttg tagaagatat ccatcaagtt gttgagatca tccgtaaaag aaaagacatg
     1801 aaagttctcc aagaacgaga ttacatcaca aataaaaaag caagtggcta ccgttcttat
     1861 catctggtga tcgagtatcc tgtacagctg atcagcggtg agaagaaaat tttggcggaa
     1921 atccagattc ggactcttgc gatgaatttc tgggcaacga ttgagcattc gctgaattat
     1981 aaatatcaag gcgtttttcc agaagaaatg agtgaacgac ttcagcgtgc ggcagaagct
     2041 gcctatcagt tggatgaaga gatgtccagt atccgtgaag aaatccaaga agcacagcgg
     2101 ttattctccc atggaagagg aaaatcagat gatgtctact atcgaacgct tttgaagaaa
     2161 gaaagcgaaa gagaggaggc aggggatgaa agtagcgatc gttcataata aagaagcaaa
     2221 gactatagaa gtgacagaac gtttgaacgc acttttagaa caagcaggga ttcaacgaga
     2281 tgatcatgag ccagaattgg tcatctcagt cggcggagac ggcacattat tgtcggcgtt
     2341 ccatcactac agtcattgtc tgaatgatgt gcgtttcttg ggcgtgcata caggtcattt
     2401 agggttttac acggattggc gggattacga gctggaagaa ctagtagaca gcttacgaac
     2461 gaaccgggaa caaagtgtta gttatccctt gctggatgtt cggatcagtt atttggatga
     2521 aacaccggac cagcattttt tagcactgaa tgaatcaacg attaaacggg ccaatcgtac
     2581 gatggtagcc gatgtataca tcaaaaacga gctgtttgaa agttttagag gagatggatt
     2641 gacgatttct acgcctactg gttcgacagc atataataaa agtgtcggcg gtgctgtgat
     2701 ccatcctagt atcaatgcct ttcagttggc agagattgct tcactgaata atcgagtgtt
     2761 tcgtactttg ggttctccga tcgtgattgc ccatgatgaa tggctagaaa taaaattgga
     2821 aaatacagaa gattatcttg taacggttga tcaattgaat gtcgcaaaag caaatatccg
     2881 ctcaatttat tatcgaatag cagaagaacg gatccatttt gcttcttatc gacatatgca
     2941 tttttggcat cgtgtgaaag atgcctttat tagtgaggat tgatatgcag tttacatggg
     3001 attatgagaa agatcattct cagcaagtaa aatatttttt aaaagaaaaa gggatctcaa
     3061 agggtctgtt agcgaaaata aaatttcaag gcggtcaaat caaagttaat gatcaagtag
     3121 aaaatgtctt gttttctcta gctaaggatg ataaggtaac cattgtgatt cctgctgaag
     3181 gagaacatga aaccgtactt ttggatgaaa caccgatcga tatcgtgtat gaagatgaac
     3241 acgttctggt agtaaataaa ccagcaggcg tttcgtctat tcctgcccaa taccatccaa
     3301 atgggacaat ggctaatcgt gtaaaggctt attataaaaa acaaggttac gaaaaccaag
     3361 taatccatgt ggtgacgcga ctagatcggg atacatctgg cttgatgctt tttgcaaagc
     3421 atggatttgc gcacgcaaaa ttagatatgc aacttcgtga aaagaagttt gtgaaaaaat
     3481 atcaggcctt agtcagtgga aggctttcag atttatcggc acatggacat atcgatttgc
     3541 cgatcgcccg agattattct tcgctattga agcgaatggt ctctgatgaa ggacgttcgg
     3601 cacagactga gtactggata gagaagcgac taacagaaat cgcgttagtt gatatccagc
     3661 tgcatacagg aaggacacat caaataagag ttcatttttc cgcaattggc tgtgccctgc
     3721 tcggtgatga tatgtatggc gggcgaatgg atctgggaat aaaaagacaa gcccttcatt
     3781 gttatgatct gcgtttttac catccattta cagaagagtt gctggagttc aagcagcctt
     3841 tagccgcaga catggaggca gttgtgagac ggtttgaaga ataaggcgag gtgagattgg
     3901 tttggatgaa aatcaagaat tagaggaaag atttgcccag ttgactcaag atttaaggga
     3961 aaataatatt caggcattca gaaacagttt tttagagatg catatctatg agcaggggca
     4021 attctaccaa tctcttacag aagagaacag gcagctgatc tattcctatc tgtcgcctaa
     4081 agaattagcg gatatgttcg atgtgattga agaagacaac gaacatatga aagaatacct
     4141 agatgaaatg cgtcctagct atgcagctga tatgctttct gagatgtata cagataatgc
     4201 tgttgacttg cttaatacgc tggataaaaa gcaaattgct aaatatttaa gtttgatgtc
     4261 ggcagacgat gccagtgaaa tcaaggaact tctccattac gaggatgaaa cggctggcgc
     4321 gatcatgaca actgaatttg tttcgatcgt agccaatcaa actgttcgct ctgcgatgta
     4381 cgtgctgaag aacgaagcag atgtagctga aacaatctat tacatctatg tcgttaatca
     4441 agaaggtcag ctagtagggg ttatttctct gcgcgaccta atcgtgaatg atgacgacgc
     4501 aatgatttct gatctgatga gcgaacgagt gctttcagtt catgtaggcg acgaccaaga
     4561 agatgtggca caaacattta gagattatga cttcttagct cttcctgtta ccgattatga
     4621 tgatcatttg ttaggaatcg tcacagtcga tgatatcatc gacgttattg atgatgaagc
     4681 tgctagcgat tattcaggac ttgccggggt caatgtggaa gaaattaatg aaaatccagt
     4741 aaaggcagcg tctcgacgtt tgccttggct gatcactctg ttgtttttag gtatgtcgac
     4801 cgcttcttta atcagtcact atgaagactt ggtaagtgaa gcgagtattt tagcagtctt
     4861 catttcgctg attacaggaa ctgccggtaa tgctggaacc cagtcgctag ctgtggctgt
     4921 tcgtcgactt gcgatttctg atgagaaaga tcatggattt gttcgacttg ttttggctga
     4981 agtcttgacc ggcgttgtca caggagctgt tacggggatc acgatttttc taatcgtagg
     5041 tttttggaaa cataatttta tcttggggtt tgtcataggg atggctatgc tatttgcaat
     5101 cacagtggcg aatcttgcag gtagtttaat cccgatgcta atggatcgtc taggatttga
     5161 tccagcagta gcaagcggtc cctttatcac gactttgagc gatttgacga gtgtactgat
     5221 ttatttcaat attgcttctt tatttatggg ctattttatg taaaaaaaca aaaacatttg
     5281 caataagcat tatgcctgtt gcaaatgttt ttttgtttat aagattataa ctgaagatcc
     5341 acgattttgg ttccgtgtac ttcgcttacg ttcaattctt tagctacatg tgctgcattc
     5401 tctgcagtga caccaccacc aggcaaaatg attaagcggc catctgtata ggcaatcaat
     5461 tctttaagcc gtgggaaatt ttctacaata gttttgtcag caggaccgcc atgagtcaag
     5521 atacggtcaa caccatgttc taccagccaa tcgattgctt cgaattgccg atcttcagga
     5581 atactgtcaa aagccatatg gaaggtgatc tgcaaccctt ctgctgtttc gatcaatagt
     5641 tctaaggcct cttcgtctag ccagcctgaa ggagtaagac aaccaaatac tacgccatcc
     5701 gtccctaatt tctttgcttc aatcaaatca gtatgcatga ttttcaattc gatatcgtta
     5761 tagacgaagt cgccccctct aggacgaata atcgtcatta cggggatatc tttttcccca
     5821 gcataactta acgtttcttc gatcacacca gtactcgggg ttgtgccacc gaccgctaag
     5881 ttgtcacaca attcgatccg atcggctcct ctagcaattg ctagaggaat agatgtgtag
     5941 ttttcagcgc aaaattcttt gatcatctaa tcttctcctt tatctttcac tttcttctag
     6001 gcattgtatc atatttttag cagttgccaa agtgagcatc taacggtaag ataaaacaga
     6061 aatgaggggg aactatgtta aaaaaaattg accgaggacg ccagtttttg gcagaaaatc
     6121 atcagctgtt ccgctgtccg gtttgccaag aagaagtaaa aaagtctgat aatggcttga
     6181 tatgcaaaaa t
//
LOCUS       DAZUKT010000126         6174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000126 DAZUKT010000000
VERSION     DAZUKT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.132"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG816_002786"
     CDS             725..811
                     /locus_tag="ACG816_002786"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4921605.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG816_002787"
     CDS             complement(932..1621)
                     /locus_tag="ACG816_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921606.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG816_002788"
     CDS             complement(1628..1906)
                     /locus_tag="ACG816_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921607.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG816_002789"
     CDS             complement(1866..2780)
                     /locus_tag="ACG816_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4921608.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG816_002790"
     CDS             complement(2762..3142)
                     /locus_tag="ACG816_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4921609.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACG816_002791"
     CDS             complement(3593..3880)
                     /locus_tag="ACG816_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4921610.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACG816_002792"
     CDS             complement(3900..4124)
                     /locus_tag="ACG816_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4921611.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACG816_002793"
     CDS             complement(4579..4749)
                     /locus_tag="ACG816_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921612.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACG816_002794"
     CDS             complement(4764..5297)
                     /locus_tag="ACG816_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4921613.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACG816_002795"
     CDS             complement(5318..>6174)
                     /locus_tag="ACG816_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4921614.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1631 a         1155 c         1039 g         2349 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccaac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag cttactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtcgactat gccaagtagc
     4321 ccctctatca agggctatag ctgctcactt tgtgattttt cattggtcta ggtcaaaagc
     4381 tttttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAZUKT010000127         6023 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000127 DAZUKT010000000
VERSION     DAZUKT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.133"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            641..1240
                     /locus_tag="ACG816_002796"
     CDS             641..1240
                     /locus_tag="ACG816_002796"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4921615.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            1256..1732
                     /locus_tag="ACG816_002797"
     CDS             1256..1732
                     /locus_tag="ACG816_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921616.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            1749..2651
                     /locus_tag="ACG816_002798"
     CDS             1749..2651
                     /locus_tag="ACG816_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921617.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            2724..2915
                     /locus_tag="ACG816_002799"
     CDS             2724..2915
                     /locus_tag="ACG816_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921618.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            3079..3666
                     /locus_tag="ACG816_002800"
     CDS             3079..3666
                     /locus_tag="ACG816_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921619.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            3666..4565
                     /locus_tag="ACG816_002801"
     CDS             3666..4565
                     /locus_tag="ACG816_002801"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4921620.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            4565..5479
                     /locus_tag="ACG816_002802"
     CDS             4565..5479
                     /locus_tag="ACG816_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921621.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            5531..5746
                     /locus_tag="ACG816_002803"
     CDS             5531..5746
                     /locus_tag="ACG816_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921622.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         2306 a          769 c         1212 g         1736 t
ORIGIN      
        1 accttggtca caggcagaaa ttgataaagc agtaaaattg aaccgattat caaaatttaa
       61 cgtattagga aatgcctttt aaaaatcgca atacacaaac agaaaaatac caaaaaatta
      121 gcaatgaacg taggtaagaa catgacaata aataataata accctaaaaa atataatgct
      181 cttgtccaat gctatatcga aaacaaaatt acaatgcaag aagtcgctga tgaattaggc
      241 aaatcaaaga gcctagtatc acacgatttt aagcacaatg tttttccgaa cccgattgag
      301 ctgaggaaaa aatttcaaaa gagagtttat caagaaatag gtaagtggat tgatgaaggg
      361 aatgcaattg accctttaat tgaaaaatat ccttttttaa aaagcaaaaa taaaattcaa
      421 aggttaattc gtcaaggcga tattcaagct aaagaacctg ttttgacaac caatgatttg
      481 gctaaaataa atcttcataa acaaattatt gaagactttg aaaaaaatct ttcaacaact
      541 gaaattttaa aaaaatacaa tattccgaga ggaaagctaa catcgactct ttttagagaa
      601 tatggagaat cggcagttac taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa
      661 tagtagtcga tacctagtag cgactgtagt ttttgtttgt gtattgattt tcctgtttga
      721 ttttttgcag attggaaaaa gcataattgg aaatacattg agtgcaaagg aaatggttgc
      781 tttaggtggt ttgtatgaag gacacttgtt cccgaatata ttcatttcta tcgtttctca
      841 tattaacttg taccatttaa tttctaattt attgttctta gtagttcttg aagaagcact
      901 gaatgatcag tatgagtggt taacattatt gatcgtttta atcatatcag gatttctagg
      961 aagcttagtt ccttttctgc tacacagcaa catcgtaagt atggggctta gtggaggtat
     1021 ttttggatgg attgctttat ttgcgattgc atacatccgt tttgatagca agaaacattt
     1081 attactaagt gtttttgtat tattgataat cggcatttta ttggtatcta cttttgaatc
     1141 aggcgaggca aatgtcttgt cacatgtaat cggatttgtc tgtggaatga tcttgggatt
     1201 tattctaaag aacgtttcat tgatcaaaca aaagaattaa aagggagtgc taaaaatgcc
     1261 aaaaagaaaa gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca
     1321 aatactgtta agaaaaaaga ttgaaacaat agacgaagaa gttagaaata aaagggctat
     1381 gtacgaacaa cgtgaagcgc taagagaacg aatccactat tttgatgata tacacactaa
     1441 aatctatgaa gcacgtacaa atcacgaaga cgaaagtaaa ttaggattag ctcaacaacg
     1501 gatacttgaa tggcttaata cgaatactga tcaagaaccg ttaagagtga ttagtaatct
     1561 ttttgaattt ggaacgaagc attggtttaa tgctgtagag aaggatgctg acttacaaga
     1621 agcgtttgat acgctaagta aggatagcaa atccatgcga gatttattga ttacttatct
     1681 aaatgagcat gagtttgaac aaacgaaaac caaaaagaaa aataaagaat agaaagttgt
     1741 gatgaatcat gctattaacc ctgttaggtt atttatgttt aattcttgct ttttttgggg
     1801 caatcgctat aattattggt caatttcaaa caaataatcc gattatgcgt aacataggca
     1861 ttggaattgt gattgtaacc tttattgtgt ttaatgccac taagattttt ggaagtaacg
     1921 ataaacaatt tggtatatcg ggagttaata atagttatgc tccagattct ttgggaaggg
     1981 tagtaataga tggaacattt gaaaatgtaa cgagttataa aaatgtttcg ctgacagtga
     2041 aggaagatgg caagaaagca tcagacgacg ttctcgctac tattaagccg tctcaatcac
     2101 aatttgaagt gttttataca gttaaagaga atagccctgt acacaagctg acattggttt
     2161 ttaacgttga tggagaagaa tactataaag atgtaaaaat aaataaaaca tcaacgaaaa
     2221 agattatcga atcagcgaaa aaagaagtag ctacagaaga aagtacaact gaatcaagtt
     2281 ctactgtcga agcggaacta ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt
     2341 ttgaatctgt ttctggttct atggctgatc ggtttaacac tgaagtcttc aaagctatca
     2401 aaactgttaa attggatact gaaaagcaaa tgattaatgt taccttattc aattcagttg
     2461 aatctatgga tcaggataaa aaagttacgg ttgcaagaga actaggaaaa agagtaattg
     2521 gtttgtatcg tggtactgct aaaccagatt cagacggtgc agatttgcca gttcatgtag
     2581 tttatgaaga cggtaaagtt tttggagatt ctacagcaaa cgaagcatta acgattaaat
     2641 tggctaaata gccagaaagt tataataaac aaatggtgtc acaccaataa tatataggtg
     2701 tgacaccttg gagatgatag ctgatggtag taaataaaga aaaagttaga aagtatgtga
     2761 ctttaaaaat agcttctgtt aaatatttag aaaagcgagc taaagaagaa gaaagagcaa
     2821 gaggtaagaa agtaacagtt tcagagttgt tagaagaatt gatcgagaat gatcaaacaa
     2881 taagacgtgc atttactaat aggtctatca gataacaata taattaataa aaaagaaaga
     2941 gtgatttggt cttagactaa atcactcttt ttatataaag tttctttaca ctcattccga
     3001 ataatgttaa aattctttat atgatatata gttccttgtc agaggaagag ataaccagca
     3061 atattggagg gataataagt ggaaaacaat aatgatatta gacattttag cattggggaa
     3121 agagttacaa cagtaagcga attatcaaag gattatagag ataaagaggg aacgattatt
     3181 tctcttaata agaatcgaag aggcgtttgg caatatgttg ttgatttagg tgatggcgtt
     3241 acagttgagt gtaaagatag tcaactgatc agaaataatg gcagtcattc cattgaaaaa
     3301 gcaattcttg tatacccaaa agtatcaagt aagatcatga atgactggtt gcttggaaaa
     3361 tttggtgagt acaataaaaa agaaatcgca gaagtgatcg gcaaatctta tccaacagta
     3421 ttagggtatc taaaaaagat aacgagtaaa gaagcaaaaa ttgaggatgt tttaacagta
     3481 agagagcttc aatcattaat cgagtatcgg aataaacaaa taacaaatcc tgagtacctt
     3541 agaaaagaag atggtctatt gaaacaattg gaacaagtaa ataaaatccg gaagatgcaa
     3601 aaggtagctc ctttaagcct tgataccttt aaaaaaatct atttgaaaga gatcaaaaaa
     3661 gactaatgtg gactgtaact ttaccgttcc aacctgtatc tgcaccaaga ccaagtgcaa
     3721 gaaggaatgc tgctgatgaa actaatgacg tagcagagaa aaggatttca acctactatg
     3781 ataaaaagta tcttgattat ttgaaaaaaa taaagagctt tatcgaagat caagatttat
     3841 tagatgatga attttactcg attgtaaatg atcctatggg cgcaattatg gaagtggact
     3901 tttactacca gataccaaaa agctataaaa aagtctataa cattatgaag acaacagcgc
     3961 cagatttaga taatttagta aaaagtgcta tggatggtat ttttaacatc tcagctatac
     4021 gagatagcca tatcacaggc ttaattgctt ttaaatataa tgtggcgaac gaaggaaaaa
     4081 cagttgtgag gatcaaacga tattcagaat ttgagtggga tacttctgaa ggattgaacg
     4141 gagatatatg ggaagctaca tttgatttta aaccagtagc aacgccaaga ccaaagtcta
     4201 aatttagagg aaatgggatt atcacttact atccaaaaga gtacaacgac tatttggaaa
     4261 atatcaagaa tacgcttaaa gagaaagatt tggtaaatga tcaactgaaa aaggtaatga
     4321 atgctcctat gggggttata gctcagatag attattttta ccaagtgaga aaagataaga
     4381 aaaagcttga ttcactgatg cgtacttcac agccggatat tgataattta gtcaaaggaa
     4441 cattggacgg aatttttaac aaggaaatag gcatcaaaga tagcagggtt gtagggttaa
     4501 ttgcttttaa atacaatact tttgaaaagt cgaaaacaaa cgtgagacta caggagatgg
     4561 gctgatgaat aataatgaaa aaattcttca tgtacttgat agctttgaaa taattcaaga
     4621 agaactaaaa aaatatcggg atgttttgga acaaagatat gattttgtca atcagcaaaa
     4681 gagtaatcac atggatttca ttctcaatat gaatgacttg aagaagaaat tagttgaacg
     4741 aaaagaacaa gaaaagttga tcaaagctta ctttgaactc ggtgaaaaag aagttaaaaa
     4801 tgcaatggaa ctgaatgaag agcgcagggt tttagatcag ctgttagaac aattattggt
     4861 gatgtttcaa aaaggacgaa tagatgagga tttaattgaa gaaggattga gaaaatatcc
     4921 ttcaaactca ggtatcggaa tagtgctgaa agcgattgat gaagaagaaa ttgaagcttt
     4981 cataccgcca gaggattttg aatcggcaat ggaatatata aaatattata gccaagggat
     5041 cactgctttt agagagtttg atcctgaaga tgttattcat gacttgaaca acctaaaaga
     5101 atggtgtgaa agttatggag tcgatgattc cggattggac tacttgatca gtattatgga
     5161 aatcgaagaa gagatgccgg ataagcctga tccaacagat attttagaat tgatccatga
     5221 agctagaaat ccaattgcct atatttctag aggatatact gtattggaat actataagcc
     5281 ttatatatca gcgatgaacc atttaagacg tgtattaagg gaaaaacgtg aataccgctc
     5341 agtgttgaat gcaacgaata gattagaaaa agctgtttca gagctagatg cttattatcg
     5401 tgaacattac ctgcaagcag gtggtatgcc aagaaatacg aaagcaaata tcagtaggta
     5461 tataaaaaaa gctgagtagg cggagttagc ccataaaatg gcaaaaggta acctgaaagg
     5521 ggcattttga atgaaaaggg atgtttttga attgtacgta ttagaacgtc tagaagacgg
     5581 catgatggca caagagataa ttgatgaaat tgatgatatg atcagcagat ttggtcaatt
     5641 ggagttgaat gatggaaaca aaaattcggt agtgctaaaa caattttggt tgaacaattt
     5701 ttcgtataag gattttatca aagaaaaggc aaatacattg tactgactaa attcacgagg
     5761 agcagtgatg agagcttatt gctaagagca gttaggctct tttttgtttg tgtaaaatta
     5821 tttgttttat aatacacatt tatacacaaa aaatattgac agatagtaca caaatgtggt
     5881 aggatatatt tgtaagcaag ttagattaac aagttaaagg aagagcagtt atgaaaaaag
     5941 aagaactaat agatatattt caaattgttg aacgagcaaa taatatggga attatgtttt
     6001 ttgatcgtat ttctttaaag atg
//
LOCUS       DAZUKT010000128         6016 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000128 DAZUKT010000000
VERSION     DAZUKT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.134"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..358)
                     /locus_tag="ACG816_002804"
     CDS             complement(56..358)
                     /locus_tag="ACG816_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4921623.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(578..944)
                     /gene="ssrA"
                     /locus_tag="ACG816_002805"
     tmRNA           complement(578..944)
                     /gene="ssrA"
                     /locus_tag="ACG816_002805"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1115..1888)
                     /locus_tag="ACG816_002806"
     CDS             complement(1115..1888)
                     /locus_tag="ACG816_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4921624.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2032..2382
                     /locus_tag="ACG816_002807"
     CDS             2032..2382
                     /locus_tag="ACG816_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4921625.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2363..3079
                     /locus_tag="ACG816_002808"
     CDS             2363..3079
                     /locus_tag="ACG816_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4921626.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3079..4527
                     /locus_tag="ACG816_002809"
     CDS             3079..4527
                     /locus_tag="ACG816_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4921627.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4598..5107)
                     /locus_tag="ACG816_002810"
     CDS             complement(4598..5107)
                     /locus_tag="ACG816_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921628.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5097..5821)
                     /locus_tag="ACG816_002811"
                     /pseudo
     CDS             complement(5097..5821)
                     /locus_tag="ACG816_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2045 a         1047 c         1170 g         1754 t
ORIGIN      
        1 tttataagta tggtgataaa atccgttccg ggctaccttt tttaggtagc ctactttaat
       61 ctttatacct ttctggatca acgaaagtat acttcaaata gtcataacgc cgatgatcgc
      121 tccgtgcgtc tggcacatca gtcacgatat caaacaaaaa atacacatct ttcttcattc
      181 tggtttttgt tgccggaatt ttaaagtagt tcttattaga gtaatagaga ttgattaata
      241 agctatcttc gattgctaaa aagaaaactt ctgaatccca caccttataa aaatctttga
      301 caaatctatt cgaaggatca aatttaaacc ataattgtgt ttttccttcc atcaacataa
      361 tgttcacctc aaaaagagta tacgaactaa tgttcttttt gtaaagtagg aatatcattg
      421 ataatatgga tggaacatat atattaaaaa gtgttctttt tgaatgctat tttgaatgcc
      481 attttcattc atttaacgaa aatctgcgaa aataaaaaat gctcaaaccc ttataaaata
      541 aggatttaag catctagcga aatctgctga aatctcacaa tggagacggc gggagtcgaa
      601 cccgcgtcca aacacatcgc cacttaaaaa tctacgttca tagtcaactc atttaaggat
      661 tcactttaca gcttgccgag tgacaggcat tctgtatcgc tagtctgatg ttctcttttt
      721 caacttacag acggaaagtt gaaacgtatc ccactaaagt taggaccctt actcgagcac
      781 atgggcgatg ccgagaggat ctacgctaac tgtttttagg cagctaaagc gtaagagttg
      841 ttttcgtttt tagcagttat atttaactgt aacgttttta cgtagacgta acctacgaaa
      901 cgcaattcaa gctcgactgt gcctgtcgaa tccgtaacgt ccccgttagc taacaagggt
      961 tacgtgtatt tttcatacaa aattagtata gcatatattg ggataaattc aataaaaaag
     1021 cgaatcatat cccattattt atataataac tgcccctttc ttaagaaaaa ataagcgatt
     1081 ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat cagatcaaaa tacttcaatg
     1141 cgtggacaat tccgccttct gtatttttct ttgtgataaa ggtagaaagt tcttttaatc
     1201 cgtcataggc gttgcccata gcgattttgt tgtcgcaagc ttccaacaag gcaaaatcat
     1261 tcggaccatc gccaaatcca aaagtaggaa ctttttccag ctgaagttct ttttgcagac
     1321 gtttgacacc tgcccctttt gagattcctt tcttcactgt atcgatcgag taaggaccat
     1381 tccgataaaa agttagctca ggaaaatgtt cgtgatagta ttcgtcacct gattgagata
     1441 agatcagcaa catatttact gtgttttctt taaagcctaa aggatcaatt tccggaacat
     1501 ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct atgaccggta caccagattt
     1561 tctgatcgtt atagtaagaa agttcatgac cgttcaactg tacatgatca tacatttttt
     1621 gacattcttc tgaagaaaat gtttgagaat agatttcttc gccgtctact cgaatgaaag
     1681 aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc catgatagag tggatttctg
     1741 cttctgtacg tcctgtagcg atgacgggga gaacattgtt ttcttttaat gcgttcatag
     1801 cttttgccac ttcgggtgtg atttttgatt tcgcatctag taatgttcca tcaagatcaa
     1861 agaaagtgat tgcacgataa tttgacacaa ataaaactcc ttttttattg gaataagttt
     1921 atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa gaattcctga acatattggt
     1981 tcagtcaaca atttagagta tactataaga agagaattta ggagggaaag aatgattttt
     2041 ttcagtattg gtatcttatt cttaataatt ggcagtcttt ttcggattct gccctcaaaa
     2101 ggaaatctgc ctttttatgg gtatcattcg cctcttgccg caaaaacaga cgctcattgg
     2161 cggctggctc aaaagacaag cggaaactgg ttttttctta tgggcttact gatggcattg
     2221 atcggctatt atttaaagac atctgggcat acgaattatt ttctgattga aatgcttctg
     2281 ctagtatttc caattatgcc tatttttatt atgacagaaa agaaattaca gaaatatgac
     2341 ctggaaactg gagggaacga taatgaatat tttaatgatt gaagataatc aatctgtttc
     2401 tgaaatgatg cagatgtttt tcttaaatga aggttgggaa gcaactttca agtatgatgg
     2461 aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat tgggatatga ttacactaga
     2521 cctgaatttg cccacactgg acggtatgct agtaggaaga gagatccgca aagtttcaag
     2581 tactgtgccg attatcatgc tgacagctag agattctgaa agcgatcagg tgatcggctt
     2641 agagatgggg gcagatgatt atgtcacaaa acctttcagt cctctgacgc ttattgctcg
     2701 aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca agagaagaag cagtagtttc
     2761 agatgatcat tttgatatcg agactagtca tttcaagatg aatacgaaaa cacgagaagc
     2821 gtatctggac aaccggccaa ttgaaggact gacgcctaaa gagttcgatc tattgtttac
     2881 tttagcaaaa aaaccacgtc aagtattctc tcgggagcag ttgcttgaat tagtctggga
     2941 ttatcagtac tttggtgatg aacggacagt ggacgcccat atcaagaaat tacgacaaaa
     3001 aatcgaaaag gtcggaccac aagtcatcca aactgtttgg ggagtcggct acaaattcga
     3061 tgattctggt gttgcctaat gcgttatttg tatcagcaac tcttagcttt ctgcggaatg
     3121 gtcgcgttga tcttattgat cgttggagtt tcttttacac aactgacaaa acaaacgatt
     3181 gaagaaaaca attaccagca actgtttgga tatgctgaat cagttgaaaa gacaacgcaa
     3241 acctatgccg atacgctccc gcagtttagt caagatcagg cgtttcaaaa tgcattgctg
     3301 ctgacagagc aagtactgac agaacaaaat gtgaacttta tatttatcga taaaaacgaa
     3361 cgtgtcattt atccgactaa tacagcttta ctgaatttta ccttaacagc tgatcaatgg
     3421 aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga ataaaaatat tttaggacaa
     3481 gcacaggcga cctcttacgc acttgtgcca tttaatttga atcatgaatt ctatggagca
     3541 cttgtagtta gtcaacctgc gcgaaatatc gataacagcg ttcgtccaat tacactgaac
     3601 ctgttcaaag gatttatttt ttccagtatc gttgcagtta ttgccagcta tgcttttgca
     3661 gcattccaag ttaagcgtat caatcggtta aggaatgcga cgaaagaagt gacgaatggt
     3721 aattttgatg tacagctccc cgtacatgac aaagatgaat ttgatgagtt agcagatgat
     3781 ttcaataaaa tgacgaattc attgaaagaa tctcaagcag aaattgaaga acaagaaaat
     3841 cgtcgaagac aatttatggc agatgcttct cacgagatga gaacacctct tacgacaatc
     3901 aacggacttc tggaagggtt agaatacaat gcaatccctg aaaatcagcg agaaaacgcg
     3961 attaaattga tgaaaaatga aacagagcgt ctgatccgtt tggtgaatga aaacttggat
     4021 tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta aaaaattcaa tggaacagaa
     4081 acattgaaaa atattgttgc acagttagaa gctaaagcag aagcagcagg agatacattg
     4141 acattgaaag ccgttgatga tattgatgtt tacgctgatt atgaccgatt tgtccaaatc
     4201 atggtcaata ttattcaaaa cgcgatacag tttactgaaa acggccaaat cacgattact
     4261 ttagaaaaag gctatcttga aacgatcatc acgattgaag atacgggaat tggaatgtct
     4321 gaacaacaaa tgaaaagtat ctgggatcgt tattataaag tagatccttc aagaaaaaat
     4381 acaaaatatg gtgaatcagg attaggttta ccaatcgtgc agcaacttgt acgcttgcat
     4441 aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa caaaattcgc tgttcgtttg
     4501 cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta taaaagatca tgaatcgatc
     4561 ttctataccg cttgtttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     4621 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     4681 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     4741 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     4801 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     4861 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     4921 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     4981 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     5041 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     5101 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaagtg aaagggaata
     5161 gactaagaat gagacagcat ccactgagcc caataagagt ttgggcaaat cccttaagta
     5221 tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa aaaagtctgt tgatccatcg
     5281 gaattgtaag tccgcttacc gaagtcaaga tatcacggat atctggcagt tcagtagtct
     5341 ttaagacaag aaaattgatc ctttgaagaa gcgcttcata aactgactgg aagcttacga
     5401 tccacaataa aaccaagata caacaaagaa agagaggaaa gaacagctgc cagaaaataa
     5461 aaaggaataa tttggtttta gaaagaccag tcaggtgcca attgatcaat tcctgcttgt
     5521 tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa ggcaaataga aaaaataaac
     5581 tgaatatgcc ttttatataa aatcggctag ctaattctaa agccggaaga agctctgtag
     5641 atgcagaagc gccaataaat gaaagccgat cttggacttg attggctaaa gccgattgca
     5701 aatggtaaag agtcaggcat gctatcaata aaaaagcaga aaagcttgaa aataaacaat
     5761 agagtattgt ctgctttttg taataaatta atcgagcagt agcatagcgg attattttca
     5821 tacttttcac ctctataaaa agtatagaaa atgaatatga ataaagtatg aaattccttt
     5881 agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa agcaagaggt tgtgaagaag
     5941 ctgccctatt ctattaccgt agatgcaagc ttttgaacac tgtttattcg aaaagaggag
     6001 cagcgacaat aatttt
//
LOCUS       DAZUKT010000129         5999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000129 DAZUKT010000000
VERSION     DAZUKT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.135"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..477)
                     /gene="yajC"
                     /locus_tag="ACG816_002812"
     CDS             complement(97..477)
                     /gene="yajC"
                     /locus_tag="ACG816_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4921629.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(574..1719)
                     /gene="tgt"
                     /locus_tag="ACG816_002813"
     CDS             complement(574..1719)
                     /gene="tgt"
                     /locus_tag="ACG816_002813"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4921630.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1937..2635
                     /locus_tag="ACG816_002814"
     CDS             1937..2635
                     /locus_tag="ACG816_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4921631.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2803..5442)
                     /locus_tag="ACG816_002815"
     CDS             complement(2803..5442)
                     /locus_tag="ACG816_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4921632.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1685 a         1317 c         1103 g         1894 t
ORIGIN      
        1 gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg taggacatct
       61 ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct tctcttctgt
      121 ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg gttcttctgt
      181 tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga ccgttttgat
      241 tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc
      301 attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg aatcacctgg
      361 tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat
      421 aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg ttcccatttt
      481 tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg ctgataacac
      541 tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt tttctttatt
      601 gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat ccatgattgc
      661 ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg
      721 aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg tatagttttt
      781 acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat atttcgcatt
      841 tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga ttcgagtagg
      901 caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg aatcagcagc
      961 accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag tgaattctaa
     1021 tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg agtaaccagg
     1081 gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa accctgctcc
     1141 ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca atcctcgttc
     1201 tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt catcgaatgg
     1261 tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga tgttgattgc
     1321 tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat gcacgccttc
     1381 ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg aatctgttaa
     1441 aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta ctaagtcttc
     1501 accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac ccatttcttt
     1561 caactcttct ggggacattg tttttactgt agctaatgtt cctactggca tgaacattgg
     1621 tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc
     1681 tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta ctttctatat
     1741 aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc aagttccctc
     1801 ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga ataacaaaaa
     1861 aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga
     1921 aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct
     1981 aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc
     2041 tactattctt cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc
     2101 agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat
     2161 tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat
     2221 tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg
     2281 tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg
     2341 gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt
     2401 tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac
     2461 tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt
     2521 tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc
     2581 atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag
     2641 agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag
     2701 aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt
     2761 gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc
     2821 ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat
     2881 ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa
     2941 tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc
     3001 tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg
     3061 cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc
     3121 gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac
     3181 gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc
     3241 ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa
     3301 agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa
     3361 tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca
     3421 gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac
     3481 agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc
     3541 cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc
     3601 atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac
     3661 gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc
     3721 atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc
     3781 attcaagata cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat
     3841 catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg
     3901 tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga
     3961 agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc
     4021 ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg
     4081 cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg
     4141 aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt
     4201 ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc
     4261 tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca
     4321 tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt
     4381 tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc
     4441 tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg
     4501 gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat
     4561 aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc
     4621 aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc
     4681 gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat
     4741 agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt
     4801 gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg
     4861 gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact
     4921 ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc
     4981 ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga
     5041 ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg
     5101 caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat
     5161 cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc
     5221 ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct
     5281 tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg
     5341 cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt
     5401 taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa
     5461 tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat
     5521 aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa
     5581 cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga
     5641 agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca
     5701 gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga
     5761 agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa
     5821 ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt
     5881 taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct
     5941 gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct cggcttttt
//
LOCUS       DAZUKT010000130         5995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000130 DAZUKT010000000
VERSION     DAZUKT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.136"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..527
                     /locus_tag="ACG816_002816"
     CDS             9..527
                     /locus_tag="ACG816_002816"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4921633.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            655..1338
                     /locus_tag="ACG816_002817"
     CDS             655..1338
                     /locus_tag="ACG816_002817"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4921634.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1647..1720
                     /locus_tag="ACG816_002818"
     tRNA            1647..1720
                     /locus_tag="ACG816_002818"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1681..1683,aa:Arg,seq:tct)
     gene            1879..2238
                     /locus_tag="ACG816_002819"
     CDS             1879..2238
                     /locus_tag="ACG816_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4921635.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2361..3992
                     /locus_tag="ACG816_002820"
     CDS             2361..3992
                     /locus_tag="ACG816_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4921636.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4054..4695)
                     /locus_tag="ACG816_002821"
     CDS             complement(4054..4695)
                     /locus_tag="ACG816_002821"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4921637.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4881..4957
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4977..5285
                     /gene="rplU"
                     /locus_tag="ACG816_002822"
     CDS             4977..5285
                     /gene="rplU"
                     /locus_tag="ACG816_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4921638.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5312..5656
                     /locus_tag="ACG816_002823"
     CDS             5312..5656
                     /locus_tag="ACG816_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4921639.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5669..5962
                     /gene="rpmA"
                     /locus_tag="ACG816_002824"
     CDS             5669..5962
                     /gene="rpmA"
                     /locus_tag="ACG816_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4921640.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1864 a         1009 c         1281 g         1841 t
ORIGIN      
        1 tgaaaacgat acctgattac aaaaaaataa cttttataag ttctataacg ttgattatat
       61 ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata
      121 ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac
      181 cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa
      241 aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa
      301 aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta
      361 gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga
      421 ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct
      481 ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac
      541 ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat
      601 gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc
      661 aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag
      721 caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta
      781 ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct
      841 gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca
      901 atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct
      961 aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca
     1021 gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa
     1081 tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg
     1141 atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt
     1201 ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc
     1261 gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca
     1321 gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg
     1381 gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga
     1441 aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct
     1501 tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat
     1561 aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta
     1621 taattattga tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct
     1681 tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact
     1741 gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa
     1801 taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc
     1861 agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt
     1921 gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg
     1981 gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac
     2041 caaggagtgt acaatggact attaggagtg ggactacttt atggagctta tctttcttcc
     2101 gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc
     2161 agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg
     2221 ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga
     2281 aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc
     2341 atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt
     2401 caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg
     2461 tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt
     2521 tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa
     2581 tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa
     2641 tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt
     2701 tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag
     2761 gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag
     2821 gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag
     2881 cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg
     2941 cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt
     3001 tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt
     3061 tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca
     3121 ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa
     3181 ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc
     3241 ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat
     3301 ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc
     3361 ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg
     3421 tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt
     3481 ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta
     3541 ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta
     3601 tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc
     3661 tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac
     3721 tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg
     3781 taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc
     3841 aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc
     3901 acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt
     3961 tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt
     4021 aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag
     4081 tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa tagctgctgg
     4141 atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat ccttttgttc
     4201 tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa ccgtatattc
     4261 tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt ttggctggac
     4321 cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac gagtaggtgt
     4381 ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat gagtcacgcg
     4441 atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta agccagttga
     4501 agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat gcgtatcaat
     4561 ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag gtccttgccc
     4621 agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt gtatcagttc
     4681 ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa aagaaacaca
     4741 atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat tgcttgaatt
     4801 tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct ggtaagctag
     4861 tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat aagcacagca
     4921 tatgctgtca cttcggatgg cgagtctaag tatatcagaa ggaggtgctt tatagcatgt
     4981 acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa gcaatctatg
     5041 ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt atcttagtag
     5101 gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg
     5161 ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct aaaaaacatt
     5221 cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg
     5281 cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta agcgaaatga
     5341 cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc cttatggaag
     5401 tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg ggattgcctc
     5461 attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt atctttatac
     5521 cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc tattagaaaa
     5581 tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc aaattaaaac
     5641 aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac aattatttgc
     5701 ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa aacgtctagg
     5761 tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc gtcaacgcgg
     5821 tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt tatttgcaaa
     5881 agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta
     5941 tccagtagct caagaagctt aattgaatct tatgattaaa gaggttgtga caaga
//
LOCUS       DAZUKT010000131         5954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000131 DAZUKT010000000
VERSION     DAZUKT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.138"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACG816_002825"
     CDS             <1..813
                     /locus_tag="ACG816_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFS4921641.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACG816_002826"
     CDS             786..1229
                     /locus_tag="ACG816_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921642.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACG816_002827"
     CDS             2399..3367
                     /locus_tag="ACG816_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4921643.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACG816_002828"
     CDS             3384..3908
                     /locus_tag="ACG816_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4921644.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACG816_002829"
     CDS             4284..4565
                     /locus_tag="ACG816_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921645.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACG816_002830"
     CDS             4567..4983
                     /locus_tag="ACG816_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921646.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACG816_002831"
     CDS             5613..>5954
                     /locus_tag="ACG816_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFS4921647.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAZUKT010000132         5902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000132 DAZUKT010000000
VERSION     DAZUKT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.139"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..169
                     /locus_tag="ACG816_002832"
     CDS             17..169
                     /locus_tag="ACG816_002832"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921648.1"
                     /translation="MEMFNLDTFDRPAYLDRKIKAYIAFFNNERVTLDMGLAIPTEEK
                     ILQMIA"
     gene            complement(254..3178)
                     /locus_tag="ACG816_002833"
     CDS             complement(254..3178)
                     /locus_tag="ACG816_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS4921649.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDSMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3324..3899
                     /locus_tag="ACG816_002834"
     CDS             3324..3899
                     /locus_tag="ACG816_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4921650.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4113..4808
                     /gene="vanR-A"
                     /locus_tag="ACG816_002835"
     CDS             4113..4808
                     /gene="vanR-A"
                     /locus_tag="ACG816_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4921651.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4786..>5902
                     /gene="vanS"
                     /locus_tag="ACG816_002836"
     CDS             4786..>5902
                     /gene="vanS"
                     /locus_tag="ACG816_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS4921652.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVEL"
BASE COUNT         1824 a         1118 c         1122 g         1838 t
ORIGIN      
        1 ttggggtacc ctcaaggtgg agatgtttaa cctggatacg tttgacagac cagcgtactt
       61 agaccggaaa atcaaggcat acatcgcgtt cttcaacaat gaacgcgtta ctttggatat
      121 gggattagca attcctacgg aagagaagat tctacaaatg attgcataaa cgctacaaaa
      181 aaagaccaca catttttaca tgcatggtct aaaatacttt gtttttttac ctgtctactt
      241 gacagggggc agttcactct gagttaaaat ggtattctcc tagtaaatta atatgttccc
      301 aacctaaggg cgacatatgg tgtaacaaat cttcattaaa gctacctgtc cgttttttat
      361 attcaactgc tgttgttagg tggagagtat tccaaatact tatagcattg ataattatgt
      421 ttaaagcact ggctctttgc aattgatgct gtatggtgcg ttctctaagc tcaccttgtt
      481 ttccgaagaa aatagctctt gccaatccat tcatggcttc tcctttattc aatcctcttt
      541 gtatttttct tcttaatgat tcatccgata tataattcaa aataaagatc gttttttcta
      601 ttcggcccat ctcacgtaag gctgtagcta agctgttttg tcttgaatag gaacctagct
      661 tccccataat aagggatgct gaaactgttc cctcccttat agaatgagct aatcgcaaaa
      721 catcctcata attttcttta atgacctttg tatttatttg tccacgtaaa atggcttcta
      781 gttttggata ctcacttgct ttatctatcg taaataattt tgagtccgat aaatccctta
      841 ttcttggggc aaatttaaat cctaataaat gagtcagtcc gaatatttgg tcagtgtaac
      901 cggcagtgtc tgtataatgt tcctctatgt ttagatccgt ctcatgatgt aacaaaccat
      961 ccaaaacatg aatcgcatct cttgaattag tatgaataat ctttgtgtag taagaagaga
     1021 attgatcact tgtaaatcgg tagatggtgg ctccttttcc agttccataa tgtggatttg
     1081 catctgcatg tagtgatgaa acacctagct gcattctcat accatctgac gaagatgttg
     1141 taccgtcgcc ccaatagaaa ggcaattgta atttatgatg aaagtttact aatatggctt
     1201 gggctttatt catggaatct tcatacatgc gccattgaga tacattggct agttgcttat
     1261 atgtaagtcc gggtgtggct tcggccatct tgctcaagcc aatattcatt cccattccta
     1321 aaagggcagc catgataatg attgtttctt ccttatctgg ttttcgatta ttggaagcat
     1381 gagtgaattg ctcatgaaat cctgttatat gggccacatc catgagtaaa tcagttaatt
     1441 ttattcttgg tagcatctga taaaggcttg cactaaattt ttttgcttct tctggaacat
     1501 ctttttctaa gcgtgcaagt gatagctttc ctttttcaag agaaacccca tctaacttat
     1561 tggaattggc agctaaccac tttaaccttt cattaaagct gctggttctc tccgttatat
     1621 aatcttcgaa tgataaacta actgataatc tcgtattccc cttcgattga ttccatgtat
     1681 cttccgaaaa caaatattcc tcaaaatccc tatattgtct gctgccaaca atggaaacat
     1741 ctcctgcccg aacatgctcc cgaagttctg ttaaaacagc catttcatag taatgacgat
     1801 taattgttgt accatcatcc tcgtataaat gtcttttcca tcgttttgaa ataaaatcca
     1861 caggtgagtc atcaggcact tttcgctttc cagattcgtt cattcctcgg ataatctcaa
     1921 cagcttgtaa aagtggctca tttgcctttg tagaatgaaa ttccaatact cttaatagcg
     1981 ttggcgtata ttttcttagt gaataaaacc gtttttgcag taagtctaaa taatcatagt
     2041 cggcaggacg tgcaagttcc tgagcctctt ctactgaaga gacaaaggta ttccattcaa
     2101 taaccgattc taaaacctta aaaacgtcta atttttcctc tcttgcttta attaatgctt
     2161 gtccgatgtt cgtaaagtgt ataactttct catttagctt tttaccgttt tgtttctgga
     2221 tttcctcttg agccttacga ccttttgata acaaactaag tatttgccta tcatgaattt
     2281 caaacgcttt atccgttagc tcctgagtaa gttgtaataa atagatggtt aatatcgaat
     2341 aacgtttatt ttcttgaaag tcacggaatg catacggctc gtatcttgag cctaagcgag
     2401 acagctgcaa caggcggtta cggtgcaaat gactaatttg cactgtttct aaatccattc
     2461 ctcgtatgta ttcgagtcgt tctattattt ttagaaaagt ttcgggtgaa ggatgacccg
     2521 gtggctcttt taaccaaccc aatatcgttt tattggattc ggatggatgc tgcgaggtaa
     2581 taatcccttc aagcttttct ttttgctcat ttgttagaga tttactaacc gtattaaata
     2641 gcttcttttc agccattgcc cttgcttccc acaccattct ttcaagtgta gtgatagcag
     2701 gcagtataat tttgtttttt cttagaaaat ctatgcattc atgcagtaga tgaatggcat
     2761 caccattttc caaagctaat tgatgaaggt acttaaatgt cattcgatat tcactcaggg
     2821 taaaagttac aaagtcgtat tcacttcgaa tttctttcaa atgatcccaa agtgtatttt
     2881 ccctttgagg ataatgatca agcgaggatg gactaacacc aatctgtttc gatatatatt
     2941 gtatgaccga atctgggatg cttttgatat gagtgtatgg ccaaccggga taccgaagaa
     3001 cagctaattg aacagcaaat cctaaacggt tttcttccct ccttcgctta ttaactattt
     3061 ctaaatcccg tttggaaaaa gtgaagtagg tccccagtat ccattcatct tcagggattt
     3121 gcataaaagc ctgtctctgt tccggtgtaa gcaattctct acctctcgca attttcattc
     3181 agtatcattc catttctgta ttttcaattt attagttcaa ttatatatca atagagtgta
     3241 ctctattgat acaaatgtag tagactgata aaatcatagt taagagcgtc tcataagact
     3301 tgtctcaaaa atgaggtgat attttgcgga aaatcggtta tattcgtgtc agttcgacta
     3361 accagaatcc ttcaagacaa tttcagcagt tgaacgagat cggaatggat attatatatg
     3421 aagagaaagt ttcaggagca acaaaggatc gcgagcaact tcaaaaagtg ttagacgatt
     3481 tacaggaaga tgacatcatt tatgttacag acttaactcg aatcactcgt agtacacaag
     3541 atctatttga attaatcgat aacatacgag ataaaaaggc aagtttaaaa tcactaaaag
     3601 atacatggct tgatttatca gaagataatc catacagcca attcttaatt actgtaatgg
     3661 ctggtgttaa ccaattagag cgagatctta ttcggatgag acaacgtgaa gggattgaat
     3721 tggctaagaa agaaggaaag tttaaaggtc gattaaagaa gtatcataaa aatcacgcag
     3781 gaatgaatta tgcggtaaag ctatataaag aaggaaatat gactgtaaat caaatttgtg
     3841 aaattactaa tgtatctagg gcttcattat acaggaaatt atcagaagtg aataattagc
     3901 cattctgtat tccgctaatg ggcaatattt ttaaagaaga aaaggaaact ataaaatatt
     3961 aacagcctcc tagcgatgcc gaaaagccct ttgataaaaa aagaatcatc atcttaagaa
     4021 attcttagtc atttattatg taaatgctta taaattcggc cctataatct gataaattat
     4081 taagggcaaa cttatgtgaa agggtgataa ctatgagcga taaaatactt attgtggatg
     4141 atgaacatga aattgccgat ttggttgaat tatacttaaa aaacgagaat tatacggttt
     4201 tcaaatacta taccgccaaa gaagcattgg aatgtataga caagtctgag attgaccttg
     4261 ccatattgga catcatgctt cccggcacaa gcggccttac tatctgtcaa aaaataaggg
     4321 acaagcacac ctatccgatt atcatgctga ccgggaaaga tacagaggta gataaaatta
     4381 cagggttaac aatcggcgcg gatgattata taacgaagcc ctttcgccca ctggagttaa
     4441 ttgctcgggt aaaggcccag ttgcgccgat acaaaaaatt cagtggagta aaggagcaga
     4501 acgaaaatgt tatcgtccac tccggccttg tcattaatgt taacacccat gagtgttatc
     4561 tgaacgagaa gcagttatcc cttactccca ccgagttttc aatactgcga atcctctgtg
     4621 aaaacaaggg gaatgtggtt agctccgagc tgctatttca tgagatatgg ggcgacgaat
     4681 atttcagcaa gagcaacaac accatcaccg tgcatatccg gcatttgcgc gaaaaaatga
     4741 acgacaccat tgataatccg aaatatataa aaacggtatg gggggttggt tataaaattg
     4801 aaaaataaaa aaaacgacta ttccaaacta gaacgaaaac tttacatgta tatcgttgca
     4861 attgttgtgg tagcaattgt attcgtgttg tatattcgtt caatgatccg agggaaactt
     4921 ggggattgga tcttaagtat tttggaaaac aaatatgact taaatcacct ggacgcgatg
     4981 aaattatatc aatattccat acggaacaat atagatatct ttatttatgt ggcgattgtc
     5041 attagtattc ttattctatg tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag
     5101 ataaataccg gcattgatgt acttattcag aacgaagata aacaaattga gctttctgcg
     5161 gaaatggatg ttatggaaca aaagctcaac acattaaaac ggactctgga aaagcgagag
     5221 caggatgcaa agctggccga acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat
     5281 attaaaacgc cccttacatc cattatcggt tatttgagcc tgcttgacga ggctccagac
     5341 atgccggtag atcaaaaggc aaagtatgtg catatcacgt tggacaaagc gtatcgactc
     5401 gaacagctaa tcgacgagtt ttttgagatt acacggtata acctacaaac gataacgcta
     5461 acaaaaacgc acatagacct atactatatg ctggtgcaga tgaccgatga attttatcct
     5521 cagctttccg cacatggaaa acaggcggtt attcacgccc ccgaggatct gaccgtgtcc
     5581 ggcgaccctg ataaactcgc gagagtcttt aacaacattt tgaaaaacgc cgctgcatac
     5641 agtgaggata acagcatcat tgacattacc gcgggcctct ccggggatgt ggtgtcaatc
     5701 gaattcaaga acactggaag catcccaaaa gataagctag ctgccatatt tgaaaagttc
     5761 tataggctgg acaatgctcg ttcttccgat acgggtggcg cgggacttgg attggcgatt
     5821 gcaaaagaaa ttattgttca gcatggaggg cagatttacg cggaaagcaa tgataactat
     5881 acgacgttta gggtagagct tc
//
LOCUS       DAZUKT010000133         5769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000133 DAZUKT010000000
VERSION     DAZUKT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.141"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG816_002837"
     CDS             complement(<1..300)
                     /locus_tag="ACG816_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4921653.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG816_002838"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG816_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG816_002839"
     CDS             complement(1014..1304)
                     /locus_tag="ACG816_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921654.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG816_002840"
     CDS             1706..1942
                     /locus_tag="ACG816_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921655.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG816_002841"
     CDS             1949..3901
                     /locus_tag="ACG816_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4921656.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG816_002842"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG816_002842"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4921657.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG816_002843"
     CDS             complement(4797..5489)
                     /locus_tag="ACG816_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4921658.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DAZUKT010000134         5530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000134 DAZUKT010000000
VERSION     DAZUKT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.142"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG816_002844"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG816_002844"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFS4921659.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG816_002845"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG816_002845"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFS4921660.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG816_002846"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG816_002846"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFS4921661.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACG816_002847"
     CDS             complement(4028..5044)
                     /locus_tag="ACG816_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4921662.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACG816_002848"
     CDS             complement(5146..5298)
                     /locus_tag="ACG816_002848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921663.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..>5530)
                     /locus_tag="ACG816_002849"
     CDS             complement(5315..>5530)
                     /locus_tag="ACG816_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFS4921664.1"
                     /translation="YEFVGMSVRKNGRLVIKYAKDNHAKRFMREENRKIDQKEFRQQY
                     EEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1452 a         1370 c          970 g         1738 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata
//
LOCUS       DAZUKT010000135         5481 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000135 DAZUKT010000000
VERSION     DAZUKT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.143"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..193
                     /locus_tag="ACG816_002850"
     CDS             <1..193
                     /locus_tag="ACG816_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921665.1"
                     /translation="DDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNI
                     GIYYTYNPVAGTYYVVQNI"
     gene            318..2669
                     /locus_tag="ACG816_002851"
     CDS             318..2669
                     /locus_tag="ACG816_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4921666.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2749..3144
                     /locus_tag="ACG816_002852"
     CDS             2749..3144
                     /locus_tag="ACG816_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921667.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3469..4086
                     /locus_tag="ACG816_002853"
     CDS             3469..4086
                     /locus_tag="ACG816_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4921668.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4127..4426
                     /locus_tag="ACG816_002854"
     CDS             4127..4426
                     /locus_tag="ACG816_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS4921669.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4460..4627
                     /locus_tag="ACG816_002855"
     CDS             4460..4627
                     /locus_tag="ACG816_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4921670.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4672..5277)
                     /locus_tag="ACG816_002856"
     CDS             complement(4672..5277)
                     /locus_tag="ACG816_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4921671.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1880 a          766 c         1069 g         1766 t
ORIGIN      
        1 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
       61 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
      121 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
      181 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
      241 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
      301 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
      361 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
      421 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
      481 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
      541 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
      601 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
      661 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
      721 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
      781 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
      841 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
      901 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
      961 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     1021 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     1081 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     1141 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     1201 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     1261 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     1321 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     1381 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     1441 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     1501 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     1561 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     1621 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     1681 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     1741 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     1801 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     1861 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     1921 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     1981 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     2041 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     2101 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     2161 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     2221 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     2281 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     2341 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     2401 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     2461 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     2521 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     2581 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     2641 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     2701 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     2761 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     2821 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     2881 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     2941 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     3001 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     3061 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     3121 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     3181 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     3241 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
     3301 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
     3361 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
     3421 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
     3481 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
     3541 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
     3601 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
     3661 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     3721 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     3781 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     3841 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     3901 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     3961 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     4021 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     4081 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     4141 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     4201 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     4261 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     4321 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     4381 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     4441 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     4501 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     4561 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     4621 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc
     4681 attcgctttc tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc
     4741 tgcaactgga atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg
     4801 cggatctgtt gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg
     4861 agccaataat actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat
     4921 ataacttagc agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt
     4981 attaaaatct aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa
     5041 agcaactgta ttttttatat cttcataatc tcttcctaaa cgatctagtg atgtaacgat
     5101 gacgcaatca ccttcacgga tataatttag taatttttga aactcaggac gctcagtatc
     5161 tttaccactg gctttttctt ggaataattt atcaatgtta agtccttcca tcattgccaa
     5221 ttgtcgatcc aaattttgat cttgtgaaga tactcgaata tagccaattt ttcccataaa
     5281 aaactccttt ttaaaagtga tataatttac atgtaaaatt tacttgtagt tttactgcta
     5341 tcatagcatt tttaaaaata aattttatgt agacttaaaa tttacttcat ggagggttta
     5401 aatggatgaa atcaaaagta acaaaaaagc atttggcatt tatttaggag aacctagtac
     5461 agaacaatta aaccaatttt t
//
LOCUS       DAZUKT010000136         5206 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000136 DAZUKT010000000
VERSION     DAZUKT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.144"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1367
                     /locus_tag="ACG816_002857"
     CDS             <1..1367
                     /locus_tag="ACG816_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921672.1"
                     /translation="FYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALT
                     IIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQ
                     IKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSI
                     IQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLF
                     QKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAA
                     KENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGES
                     WEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDV
                     NAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKK
                     NENK"
     gene            1354..2988
                     /locus_tag="ACG816_002858"
     CDS             1354..2988
                     /locus_tag="ACG816_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS4921673.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3163..3996
                     /gene="msrA"
                     /locus_tag="ACG816_002859"
     CDS             3163..3996
                     /gene="msrA"
                     /locus_tag="ACG816_002859"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4921674.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4076..5065
                     /locus_tag="ACG816_002860"
     CDS             4076..5065
                     /locus_tag="ACG816_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4921675.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1824 a          806 c          951 g         1625 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag ttgtctctat
     1741 attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc cgaagctatc
     1801 gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc tggaaaagaa
     1861 gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta cagccatgta
     1921 ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg tttggtttct
     1981 aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag tgcggcagga
     2041 atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat gctctaccaa
     2101 aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt gttcgatagt
     2161 ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt tggtctgtta
     2221 tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa aaagatttat
     2281 tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta tcttctgcaa
     2341 aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca aataatattc
     2401 tcttctctga tattaacgat gatatttttc aaaaatagaa gatacaataa gacaagagaa
     2461 ctattttatt tatttatgac tgttttgtta ttaatcactt taactgtttc tagtaaaaaa
     2521 aatttcaatc aaaaaataat ttctcagccg ggacatattg ttgtaaacaa agaaacagta
     2581 gaggcgtata ccactaaagg aatggattat tttttatatg attattctcc tatcgcttca
     2641 ttggagaata aggagcatat aataaaacac gaagcaaaga ttggcagtca atgggtaaac
     2701 cttccttata aaacaactgg tagttctatc atatatagag taactaatga gaagagccaa
     2761 gtaatcacac ttcctgtata tgcttattta ggaacgaatg tagaaataaa tcatcaacct
     2821 gtaaaggagc aggtgaatgc taatggattg gtacaaataa gcggagtcaa aggagttgct
     2881 gatattaaag taagcacaag ttatacagta acgactatta tttcttatct tgttacatta
     2941 actgcatatt tatgtttatt ttttaaaata gttttccata aaaggtaaat taacttttca
     3001 tttataaaat aaattgatta aaaaggagaa tctgtcgata aggattctct cttttttata
     3061 cgaaaaatct gtagggggat gaagtaatcg cttatttcct cactttatga taaattacag
     3121 ctaaagtcac tagcttgtct tcttcggaaa ggaggagaat ctatggatga ggaacaaata
     3181 ttcattcaaa cactttataa tctgattttg aatccgaata cacgagattg ggaacgaaaa
     3241 gtattgattc agacaaagaa tgacataaga gaaaatatat ccgttaaaga acagttatca
     3301 aaactagaag caacactacg tccattagct atccgaatga atctgacgcc tgatgtaatg
     3361 gacttttatt tattgattac ggaaggattt gataaggagc aaaaatatga cttttccaag
     3421 catgccatgc aagatgctga ctatcaagaa cgtgcagtat ttgcaggggg atgtttctgg
     3481 tgtatggtcg agccgtttga atcgaaaaaa gggatcctat ctgttttgtc aggatacacg
     3541 ggcggacatg ttgaaaaacc aaattatgat caagtaagtg gtggttacac aggtcatgtg
     3601 gaagctgtgg agatcattta tgacacaaga gaaataagtt atagcgagtt gctgacaatc
     3661 tattggcaaa ttactgatcc cacagataca tttggacaat ttcaagatag agggaagcaa
     3721 tatcgtccag tgatttttta tcaaggcgaa cgacaaaaag aattagcgga acaatcaaaa
     3781 cagaaactag acagttcagg aactttccac caaccaatcg tcacaaaaat cgaaccagcc
     3841 ggtacgtttt ggccagcaga aaactatcat cagcaatttt acaaaaaaca accgaaacgt
     3901 tataaaaaaa tccagcaagc acgtaatcaa tttctgatct atcaacgagt aaaaaacaaa
     3961 tggcaaaaaa atatacgaaa aaatcatttt gattaactcc ttttctttaa aatgtagtaa
     4021 actaaaaata gaaaaaagaa agcgttttaa aaagatttga tgaggaggag aaaaattgta
     4081 tattgcagat agtgcacgtt atgaaaaaat ggaatatcgt aaatccggtc attcaggttt
     4141 gaaattaccg attttatctc tcggattatg gcaaaatttt ggtgattatg atcctatcca
     4201 taatcaaaga gagattttaa gaggcgcatt cgacatggga attacccatt tcgaccttgc
     4261 gaataattat ggaggccctg ctggagctgc agagaaaaac tttgggcgta tttttagaga
     4321 agattttcaa gcctaccgtg atgaactgat tatttctagc aaagcaggtt atcatatgtg
     4381 ggaagggcca tatggggaat ggggctctcg taaatccatc atttcaagtt gtgaccaaag
     4441 tctccaaaga atggggctag attatgttga catcttctat catcatcgcc cagaccctga
     4501 tacacctttg gaagaaacag cagaagcttt gatgcaactt gtaagacaag gaaaagcatt
     4561 atatattggt atatccaact ataatggaga agatacaaaa aaaatgactg aaattttaaa
     4621 aagaaaggaa gcaccgttca ttattcatca gatgcgctac aatatgtttt ctcgtgcact
     4681 actagaagac gatctatctc cagtattaga agaagaaggg ctaggggcaa tcacgttcag
     4741 tccgcttgct caagggttgc tgacaaatcg ttatctacat ggtattcctg aagattctag
     4801 agcacatcgt aaggaaatac cattcctttc agaagaacaa gttggatcaa ctctggaaaa
     4861 aatcaaagca ctgcaaacaa tcgccgtatc acgaggacaa tcattagcgc aaatggccct
     4921 tgcatggaat ttgcgacaaa aatctgttac aagtgtactg gtcggagcaa gtcggttaag
     4981 tcagttacaa gaaagtgttc gaatgatgga taatttagat ttctcaccag aagaagaatt
     5041 gagaatcgac caaattctag aatagaaacg atgtaatcaa actttttatc catatattaa
     5101 aaaaaacaaa agatgacatc aagaatcgaa tgaagatcgt ctgttattga acatcatatt
     5161 ttttgtcgtt caaaataaaa atgatcgaag gctaggagtg aatttt
//
LOCUS       DAZUKT010000137         5075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000137 DAZUKT010000000
VERSION     DAZUKT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.145"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..327)
                     /locus_tag="ACG816_002861"
     CDS             complement(154..327)
                     /locus_tag="ACG816_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921676.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(330..728)
                     /locus_tag="ACG816_002862"
     CDS             complement(330..728)
                     /locus_tag="ACG816_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921677.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            944..1999
                     /locus_tag="ACG816_002863"
     CDS             944..1999
                     /locus_tag="ACG816_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921678.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2019..2372
                     /locus_tag="ACG816_002864"
     CDS             2019..2372
                     /locus_tag="ACG816_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921679.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2554..2841)
                     /locus_tag="ACG816_002865"
     CDS             complement(2554..2841)
                     /locus_tag="ACG816_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921680.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2854..3672)
                     /locus_tag="ACG816_002866"
     CDS             complement(2854..3672)
                     /locus_tag="ACG816_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4921681.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3898..4119
                     /gene="relB"
                     /locus_tag="ACG816_002867"
     CDS             3898..4119
                     /gene="relB"
                     /locus_tag="ACG816_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4921682.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4121..4387
                     /locus_tag="ACG816_002868"
     CDS             4121..4387
                     /locus_tag="ACG816_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4921683.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4554..5039
                     /locus_tag="ACG816_002869"
     CDS             4554..5039
                     /locus_tag="ACG816_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921684.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
BASE COUNT         1763 a          879 c          784 g         1649 t
ORIGIN      
        1 tgattcactt aatttgtcct ttaaaaagca cttattttaa tataatgtta ttttataatc
       61 ttattacaag ctgattgtta ggcgttgtcc tttcattaca tttattaacg gttattttat
      121 tgtttaattt attaattatt tttgatctaa aacttagctt aacaaataaa taattaacca
      181 accgccagat aaaaatgcga tcattgctaa tacataagca aagataaaaa tatgttcgat
      241 cagcttaaac cctgatgcga ttaagcagac caagtaaaat agaaattctc ttacagataa
      301 tggtaatttt ctaactagca tactcataat taacacccca aagtaatatt gtattttgaa
      361 cattttacat ccaatacagc atcaatcaac cattgccaat caaaaataca aggcttcatt
      421 gaagtatgtt tttcagatga aaagatataa tataatttct tcggaataat attttcagtt
      481 gttgggatca agctaaatct aataccgtct aactcatagc taccagcttt ctctccatta
      541 aaaatttcct tagcactttc ttctaaagaa tcaaaatatt tttcgacagc aatctttgaa
      601 gcttttacta ttgaagcatt tcccttttcg ataaaatctg cttttgtttt accgacaata
      661 agtaattctt gatcagtaaa atcttctaaa ataatggtag tgtaattctt atttcttctc
      721 aatcgcatca ggatcactcc ttaatataat ttatgttttt ataatatcat aataagataa
      781 atttgcaagc atttatttac acctttttat ttacacttta ttataattaa ttagactttt
      841 atgattgcat ttttttctta taaacagttg caactaatag taaataggtg taaactattt
      901 tcgtaccgaa ataccataaa cacaaacaga aagaggtcat accatgaaag aaccattatt
      961 gttttccgtt gacttaggga ataaatcagt aaaagaaatg agtacacacg tttctccact
     1021 ctcacttccc tctcgtattt tgaacaaaaa tatcattaat tcaggacgtt tttctaatat
     1081 aggttcctca ttaaaacgat cactaaacgt ttctgaatat agcctagtaa acactactga
     1141 aagctattac tttgggaagg atatacacga gctaggaaaa gatgactacg tgatagaatc
     1201 actaggtttt gggatcagca gatacaagca ggaaacctac caaaatatcc tatctttctc
     1261 tttagctaac ctagcacgtt tagaagatca atctgatgac gttttaaccg ctaatctagt
     1321 tttaggtctg ccttcatccg actataccga aacaatcgtc aaatacgtga tgcaacaagc
     1381 cacacgacaa cattcagttg aagttgacgg taaagcttat aatataaacg tagataccgt
     1441 ttgggtactg ccacagccga ttggaacact atataacctc ctacttgctg atgatggtag
     1501 tgtacaagat gaaacattga tcagtcagaa gatcggtttt gttgatattg gtggtggcac
     1561 gaaacttttt gatgttgtcc aaaattttga aattgacgaa aacaaccgtg aacaaaagga
     1621 taccggtatt tttactttat ataatcgtat cgtaaatatg cttgaccttc cgatcagtcc
     1681 taatgctttc caacttgaaa agattatccg agaaggcaaa gaaaaaggcg tatatctatt
     1741 taaaccaagc ggaaatgaaa gttttgatat ttcagaagta gttgaaagag aaataaaaac
     1801 atacaccaat atggttgttg cagaagtttt agctacattc aaaaacttca caaccttaga
     1861 taaagttgtc tttactggtg gtggggcttc tattatcgac aaacaacttg taaaagataa
     1921 aattccgcat accttgtttg ttgaagacag tgaattttct aatgtaaaag gcttttacaa
     1981 gtttggttta actcaataaa acagcgaagg agatcaacat gaataaggga aaatcagagc
     2041 gttatgtagt tataatcaat agagaaaagg ttagcgagtt acctttagca gagtttcttg
     2101 aatctgaata tgaaacacta ggcgttaaat actctcagac gatcaaaaaa gctttgaatc
     2161 tatatcggcg tgtaaagaac aatgaagtta caattattga agcaggcaac gatagaaacg
     2221 atagcaaagt agctgaagta aagaataaac caactgatca taaccctatt caacaagcgc
     2281 atccttcaac ggttacatcc gatcattcag caaagaaaga aaagccaatg ctaaaagggt
     2341 tcaatgcacc aagtaacaaa gacttacttt aaaaataaaa aggcgaggtt acaataccta
     2401 gcctttttat tatgtacaaa tacaaacata aacatatacg tatacatgta tataaacatg
     2461 tatacgtata tatccataaa cacgtatacg aaatttagtt aagctatacc taaccgctta
     2521 ttccaataaa aaaagtgatc agctttaaac tgatcactca ccaaaaactt catcatacaa
     2581 gttatctatc atttcaccaa gtaaagctga atctgatcga attcctaact tttcgctgat
     2641 tttagatagt ttctttcttt gtgatggttg aagcgtgaat tgataatttt tcttcttttc
     2701 agaactatca ctttttagtt tagctaccaa agaacttgtt gtttctttag gtgtttgttt
     2761 aggttcttca actgcttcaa ccacttcagg aacttcttct acttcagctt cagcgaattt
     2821 tttaaatcta ttttctttca cttcaatcac tccttattct tgtgccgtat caattatttt
     2881 gttgtatagc gtaaacgtgc tttcaaagaa atccttattc ttacgataga tattcttatc
     2941 cttagcgaac tcagcaatat tcttaccttc taagctagtt gcattaagca attccttctc
     3001 ttgtacatat ccaagataat tagataacgt tggtaccaat tctaggaact cacgacttga
     3061 attagtatta tgtttgatcc tgctaccgat cgtagcgtat ttagtttcca tcagtgtttc
     3121 accagtctta atatcaatca actgttttgc tagtgcatca atatctttac caagcttaag
     3181 caaagcttca aaaccatctt tagatggatc agaaatgcct acgacaagat cagatacagc
     3241 ccaagcattt tgagctagta gacctgcatc attatgagta tcaatcagaa ttagatcata
     3301 cttctcttta agctgttcat agttaacaac gatccatcta aacagtaggg ttctgttgtt
     3361 aggcatatca cgcatttgca tctcaacttg ttttaaatct tcacctgcta ctaaaatatc
     3421 catgttctca tttatagcta aaggttcagc tgatccatga agatataaat gattgattgt
     3481 attctctctt ttcgctttac tgaaagcatc aggaaatcgt tttgataggt tttgcgactt
     3541 atcttcatca atatacagca ccttcatacc taaattttcg acagcatatc cacctagatt
     3601 gtagattgtt gttgttttac ctgttccacc tttttcaatg tttccagtaa tgattgacaa
     3661 cttcttttcc atgattaaac ctcccttatt aatcttttgt aactccatta tacactataa
     3721 acacgtttat gtatatattt atatacataa acgtgtttat atttatattt gcttttatat
     3781 tctacaacat aaacatatat atgtatatgt ttatgtttat gtatatattc aaatatataa
     3841 ctattgcatt tgtcatacaa tcgtagtaca atgtaattga aaaggagttg ataaactatg
     3901 ccagtaataa gtattcgtgt atctaaagaa gaaaaagatt ttcttgaaaa agttgctgaa
     3961 ttcaacggag ataacatttc tgaatttatg cgtagtaatt ccctgcaaac agcagaaacg
     4021 ttagtagact ttgccaccta caagcaacta atgaaagagc atgaggaaaa cgatcaaagt
     4081 atttcccacg aagatatgct aaaagagtta ggcttataag atgacttacc aagtaagata
     4141 tgaacgtgaa gctcaaaaag cgctaaaaaa aatggataag tttcaggcaa aggttatcct
     4201 aaactggatc gagaaaaatc ttgtaggcac tgatgatcca agaaagcatg gtaaaggtct
     4261 aacagctaat aaatcaggct actggcgata tagggtaggt gcataccgta taatagctga
     4321 tattagcgag catgaagtga caattcttat attgtctatt ggtcacagaa gtgaagttta
     4381 taagtaacag acaagctgat aaggcttgtc tgtttttttg ttaacccaac aaaatcactg
     4441 tttacgatcc cactttacct aaaaaagaac aatatcctct tgatattata cacaccagtg
     4501 tgtataatat aagtgtaaac aagctaatta actttcaaaa ggagcgattt tttatgggat
     4561 ggtatggtga ttattcatca gcaaatgaag tgatcgaaga aaagaaaggg tacgccaaaa
     4621 atggtttgga aatcgtaggt accaaagtaa cgaagaccta tggaattatc cttttcaaag
     4681 atgcagaagg aacctacgat attgacagct tcatctttcg agatgaaatg tacaaaccaa
     4741 tctacttctt aaatgacagt cctgcttact ttaagaaaat tccgaaaaaa tggttgaaat
     4801 tctttgtcgg tgatcaagaa aaaatggtta agctacaact agatagaatt aaagaagaaa
     4861 gcaataaacc aaaacttgat gacttattag ttgcaggtaa aacttactca atctgggatg
     4921 gatcgtatgt cgctgtctat cagtataaac tcaaacgtag ctttattttt cgactacaag
     4981 atggatcact cactcggttc actcgactat cagcaggaga tgttaaggaa gtaacctaaa
     5041 acttaaatac ataaacataa acgtgtatac gttta
//
LOCUS       DAZUKT010000138         5028 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000138 DAZUKT010000000
VERSION     DAZUKT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.146"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..792
                     /locus_tag="ACG816_002870"
     CDS             76..792
                     /locus_tag="ACG816_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4921685.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            811..2394
                     /locus_tag="ACG816_002871"
     CDS             811..2394
                     /locus_tag="ACG816_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFS4921686.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2523..3107
                     /locus_tag="ACG816_002872"
                     /pseudo
     CDS             2523..3107
                     /locus_tag="ACG816_002872"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            3053..3571
                     /locus_tag="ACG816_002873"
     CDS             3053..3571
                     /locus_tag="ACG816_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921687.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3594..4619
                     /locus_tag="ACG816_002874"
     CDS             3594..4619
                     /locus_tag="ACG816_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4921688.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            4868..>5028
                     /locus_tag="ACG816_002875"
     CDS             4868..>5028
                     /locus_tag="ACG816_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4921689.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1857 a          576 c          982 g         1613 t
ORIGIN      
        1 cattatttca atgattaaga gtcgagagca caaacggtag acgatgttat ttttataaaa
       61 agatataatt tattaatggt cgataattat gaaaggaaaa atattcttaa aaaaaagagt
      121 ataggatgtc gtatgaaaat agtcgctata gtaccaatga agttaaataa caaaagattg
      181 ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat tgtgttatta tatactttca
      241 acacttttaa aagtaaaaca gattgataaa gtgtatgtct attgtagtaa cccagatatt
      301 aaagaattta taccagaagg tgtaacattt ttaaagagat ctagttcctt agatcaggat
      361 acaacgagta ttacacaggt attagccggt ttcacaaaag aagtagaggc tgatgtatat
      421 gtaatgacac acacaacagc accatttatt agcgaaaaat ctattgaaaa aggactagat
      481 gccgtgctga caggagaata tgattctgca tttgctgcaa aaaaattgca agatttctta
      541 tggaaagacg gtgaaccatt taattatgaa ttggacaata ttcctagaac acaggatttg
      601 ccgcctttat acgaagaaac tagtggattt tatatttata atcgcaatgt gatgaccgag
      661 ttgcatagaa gaattgggaa aaaaccttgc atagttgaag taggtgagat tgagtcagtt
      721 gatattgatg aagccgagga ttttatgatt gctgatgcga tttataatca tcttatttta
      781 caaggtagtt aagacagggg gaaggttagg atgaacaatg tacagatttt agattgcaca
      841 cttagagatg ggggctattg taacaattgg gaatttggat ttgataatac aaaaaaaatt
      901 atttctggac ttatagaatc aggaattgag attatagaat gtgggtttct cacaaataaa
      961 acagtataca ataaaaatgt tacaaaatat acgaccttag aagaattagc acagataatt
     1021 cctgaaaaaa gagaaggaaa attatttgta gcaatgatga actatggcga atataatata
     1081 gaggatcttc cagattatga tggttcatct attgatggaa ttaggattgc gtttcataaa
     1141 aaaaattgca atgaagcgtt gaaattatgt cgtgagataa agaaaaaagg ctacttagtt
     1201 tttgttcaag ctatggtatc tctcaattat acagatgaag agtttttagc agtaataaaa
     1261 aatgtgaatc agtttaaacc atacgctttt tatattgttg atagttttgg aatgatgaaa
     1321 ggaaaagatt taactcgtct tttttacatg attgagcata atctagaaaa agatatacag
     1381 ataggttttc actcacataa taacatgcag cttgcatatt ctaatgcaca aaaacttata
     1441 accgtacaaa caaatcgaag cctgattatt gattcttcgg tgtacggaat gggaagagga
     1501 gcaggtaatc ttaacacaga actatttgta gaatatttga atgataatgc aggaaaaaaa
     1561 tatcaattaa aaccattatt atatataatt gatgaaatac taaatagttt ctatcagaag
     1621 agttattggg gatattcatt accaaactat atttcagcag ttcataatgc tcatcctaat
     1681 tatgcaagct atctgtcata taagaatact ttaacaattg aagcaatgaa tgaaatattc
     1741 gaaatgatgg atgaagaaaa aaaggtatct tatgataaac aatatataga agatttatac
     1801 tacaaatata tggaaactgg gaaaatacag gaggaatata tggaagaact gagaaaattt
     1861 ctctcagaca aaaaaattct actgattgca ccaggaaaaa gttctgtaga tgaggtagaa
     1921 agaataaaag agtttgttac caaagaagaa gtcgtaataa ttagtgtaaa ttttgaatat
     1981 tctcatatag atatagatta cgtattttta agtaatttaa gacgttttca tgaattaccg
     2041 aaagaaagca gaactaaatg tattgttaca tcaaatatac aagcatcgga tgtatattta
     2101 cggacgagtt ataaaaagtt gttatctgaa agggaagttg taagagacaa tgcaggatta
     2161 atggcaatta gatttttagc cgatatgaat gtggatgaaa tattcttagc tggatttgat
     2221 ggatattcac ataatgaaga tgagaattat ggtgagcaat ccatggaaat tataacaaaa
     2281 tttgcattat tagatgcaat gaacgtgcaa atgagagatg aattgtcaga attagctaaa
     2341 aaagtaaagg tgacattttt aacggatcca cgttgggttc gaattgataa gtagtagtaa
     2401 taatgttttt aaaattttga taaagagata gtgagagaat aaaaatgggt aaagtcttaa
     2461 ataaaaaaga agaaaaatat ttaagggccg atggggcgag atgaaactct aattttgaac
     2521 ttttaagaat catttctata ttgtgtattg ttgcttacca tgctaatttg cgtttaaatt
     2581 ataatgcaga cggaattata gtacatggtt cattaactgc taaccagatt ttcttgaatt
     2641 atattggtat ttggggtcag ctaggagtta attgttttat atttatttcg gcatattttt
     2701 taagtgagaa aagaaacatt cactttgaaa atgttttggg aattgtatta gaatgtattt
     2761 tttatagtgt attagtttat ggaattcttt gtgcatgtgg aatagaaaca ttttcattga
     2821 acagctttgt aaaagaaata ttatcatttg ctctaggtag atattggttt attacatcct
     2881 atttagcatt gtatattcta agtccgtatt taaatatcat gcttaacaat ctggaaaaac
     2941 ttcagttgca aaaaataagt atgttattgt ttataggtgt atgctgttat cgtatgttat
     3001 ttaaaacatc tttgtttggg gattgtgctg ttttaattgc actatatgtc atatgttata
     3061 cactgaaaaa aacgataata atatttttga gagacacaga atcataggaa caattgtatg
     3121 tataacagtt ataccacttc tcatatccat aggaatactt ttcaaattta taccaaatgc
     3181 aatggttaac cagatggttc atattggttc tccattgatg gttatagcaa gtatattttt
     3241 attttatgta tttaaaaatt tttcatttta cagtaagtat ataaataaac ttgcgagaac
     3301 cagtttaggg gtatatattt tacatgaaaa tcattatttc attgatggta tgaagtctag
     3361 aggcttaata tgggatggga tatttggatt aggcaaaagg tttttagata gggatttttg
     3421 gatttggttt ataattgcag ttgtagttgt gtgcataatt tgcatggcta tcgatgttgt
     3481 aagagaaaaa tttgaaagac agatttttaa aattaagagt gtaaagaacc tgtgtaaact
     3541 tatgaatcat gttataaata tatacccata attaataaat gaggagaaaa gaagtgaaag
     3601 ttatatcatt cggagaatta ttattgagac ttgcagcacc cggttattca aagttatttc
     3661 aaagagatag tttggagact acattctgcg gaggggaagc taatgtagcg gtatccttag
     3721 caatatttgg atatcaatca gaatttataa caaagcttcc ggattcagat gttggatatg
     3781 cggcagctaa tgctttgcgt tattttggag taaatgttga taatattata tttggagagg
     3841 gacgcatggg actgtattat ttagaaaaag gagcagctca gagaccatct aaagtaattt
     3901 atgacagaaa attttctgca atttcgttag ctgatagtga tgaatttaag tggaaggaga
     3961 tcctaaacga tgcaaattgg tttcattgga cagggataaa tccagcattg tctgagaatc
     4021 tctttgagac gtccttagat gcatgtcagg ctgccaaaaa aagaggtata acaatttctt
     4081 gtgatttaaa ttatcgatcc aagctttgga catcagagga agcaaaagaa aagatggaac
     4141 agatactacc attggttgat atttgtattt gtaatgaaga agatgcagaa aaagtgctag
     4201 gaattaaagc tgagcataca aatattaata gtggagaatt aaataaagat ggctatgtgc
     4261 aggtggctag ggagattcac aagaagtttg gatgtaggta tgtagctaca actctgcgta
     4321 gaagttattc ggcaagtaga aatggttgga gtgcaatgtt atacgaagca gaaaaagata
     4381 aagcgtattt ttccacagaa tacaacatac aaattgttga tcgcgttggt ggtggagaca
     4441 gcttttctgc tggtataata tatgcgttat gtgaaaatat gtccggacaa aaggctattg
     4501 aatttgcgac tgcatctagt tgtttaaaac atactataga aggtgatttt aatcgaacta
     4561 caattgagga tgtatataat ttaatgaata gcggtggaaa tggtcgcgtg ttgcgttagt
     4621 caacatataa agataaatca cagtaatttt tatggtgatt atctgtctta gtagtgatta
     4681 gaataacgtg aaaataaatt gttgagcgtt tatactgttt tgaaaaataa atttaacata
     4741 ttgaaaaata caaatatata cgtatattat aaagcgccga ttgtaaaatt aagctagaca
     4801 actaaaacag tcatttgtgc tacactcaaa atagttccga ccaaagaatt ataaggagtg
     4861 agtgcaaata acctatacct atcttacaac ggatgaactt gtaataatag agtcttattt
     4921 gaaaattaac caatctgttg ctaaaactgc gcattgcttg aatcgttcaa gtcaaacgat
     4981 ccataaagta tatctgttct tcaagcaaag aaaatcagcc ttagaata
//
LOCUS       DAZUKT010000139         4999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000139 DAZUKT010000000
VERSION     DAZUKT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.147"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACG816_002876"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACG816_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4921690.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="ACG816_002877"
     CDS             complement(2065..3063)
                     /locus_tag="ACG816_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4921691.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="ACG816_002878"
     CDS             complement(3289..3942)
                     /locus_tag="ACG816_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4921692.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACG816_002879"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACG816_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4921693.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1484 a         1032 c          763 g         1720 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatgaaagt tatctaaggt taatatacag taaaaccagc attctcgctt tttttatcat
     4861 ttaaaaatta gagttttcaa atctaattca aattttctaa acatatgaaa cagcgtataa
     4921 aactgtacta ttattttatc tcaaaagcta aatgaaaccg atttcttaag attttttatg
     4981 ttttttattt ctttaatag
//
LOCUS       DAZUKT010000140         4929 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000140 DAZUKT010000000
VERSION     DAZUKT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.148"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..971
                     /locus_tag="ACG816_002880"
     CDS             84..971
                     /locus_tag="ACG816_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4921694.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1111..2037
                     /gene="cysK"
                     /locus_tag="ACG816_002881"
     CDS             1111..2037
                     /gene="cysK"
                     /locus_tag="ACG816_002881"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4921695.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2273..2719
                     /locus_tag="ACG816_002882"
     CDS             2273..2719
                     /locus_tag="ACG816_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4921696.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2992..4344
                     /locus_tag="ACG816_002883"
     CDS             2992..4344
                     /locus_tag="ACG816_002883"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4921697.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4515..4802
                     /locus_tag="ACG816_002884"
     CDS             4515..4802
                     /locus_tag="ACG816_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4921698.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1694 a          813 c         1030 g         1392 t
ORIGIN      
        1 attaaaagta taatttaatt aattaagttt gttttaagaa gagaactttt tattaagttt
       61 ttttattttg aggaggaatt tgaatgcaag agctgaagac ccgcagtgcc cggcaccata
      121 gacctaaaag aaaaatggtg atcaataaaa aagcgattgc tcctttaatt ggaacgagtc
      181 ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca gatgaacaag
      241 gatctcaaac agtcgctgat cctcaagcgt ttatcgatca aatcggctgg tctgcgtcag
      301 aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg ttgttggaaa
      361 gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc ggtgtgaaag
      421 gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaaac ggacaatggg
      481 taacgatgaa tgagccattc cgaaaatatt cttcttattg ggaatccttc caagaccatg
      541 cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct ggcgtgtgga
      601 aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt tatgctaccg
      661 atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta acccgttttg
      721 atacgccatc aacaggttcg gctacaacaa ccacaagtac aacaacaacg actactacga
      781 cgacttcaac gtcatcatct tcaacaacag atgtaaatac aaataatggt cagacatata
      841 cagtcgtgtc aggtgatacg ctatgggata ttgcacaaaa atttggcatg tctgttgatg
      901 atctaatggc gaaaaatggc atgtctatgt ccagttattc tttattagtt ggtcaagaac
      961 taaacgtttg atttagataa aaaggcaaag aaattaccat atatggtata tctttgtctt
     1021 ttttttgcta gtcaaagacg aatcactcgc cataaatgaa cagattgtat tatgataagt
     1081 aagaagagca aagacaagga ggaattgaag atgacagcta tctataactc agtcaccgaa
     1141 ttgattggca aaacgccaat cgtcaaatta aataaaatcg taccagaaga ttcagcagat
     1201 gtttttgtaa agcttgagtt ttttaatcca ggaggaagtg taaaagatcg tattgcttta
     1261 agtatgatag aaaaagcaga acacgatggt ctactgaaac caggtgatac tatcattgaa
     1321 cctacttctg gaaatacagg aatcggattg tcgatggtag gagttgcaaa aggatataaa
     1381 gtaatcatcg tgatgcctga gacgatgagt atcgaaagac gtttattgat gaaagggtac
     1441 ggcgcagagt tgattttgac tcctggtgcg gatggtattt ctggatcaat cagagaagca
     1501 gaacgtttgg caaaagaaaa cggctacttt ttaccattac aatttgaaaa tgaagcaaat
     1561 cctcttgtgc atgagaagac gactggtcct gagatacatc aagcatttgg agtaaatggc
     1621 ttggacgcgt ttgttgcagg tatcggtaca ggaggaacga tcactggcgc tggccgagaa
     1681 ttaaaacgtg tgtatccgaa aatcgaatta atcggggtag aaccagcaga atctgctatt
     1741 ttagaaggga aagaagcggg acctcacaaa atccaaggaa tcggtacagg ttttgttcct
     1801 aaaacactgg atacttctgt gtatgataaa gtcctttcta ttagtggaga cgaagcgatg
     1861 gaaacagcta gagaagttgg gcgaaaagaa gggattttag taggcatctc atctggcgct
     1921 gcaattgctg ccgcgctaaa agtcgcaaaa gaattaggaa aaggcaagaa ggtattagct
     1981 gtggttcccg acaatggtga acgttattta tctacagccc tttaccaaga gatataaggc
     2041 acttggtttt tctccagata ggtttatgta aaaaactttt aaagatgaaa agaacacaca
     2101 aaaatttatg atgagaaaaa ttatttgaat agagacgtag aatagtgaac gcgactgttt
     2161 tacgtctttt ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt
     2221 taaaatgtaa aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa
     2281 taagttagtc gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca
     2341 acggtacgcg gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat
     2401 ttataaagca cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct
     2461 tcgcttattt acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg
     2521 atttgatttc agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt
     2581 tgattttcat tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt
     2641 tgagattaat gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc
     2701 aaaaaaggcg caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg
     2761 tgtaaaaaaa acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa
     2821 agtgaatatt attaaaataa gaatcttgta gatattgggt tcttatgctt gaaaaggaat
     2881 gaaactacaa acaaaccttt gtgaattcac aagcattaaa ctgttgtatc cgttttattt
     2941 ctgttatagt agttttgtcg aaggacaaaa tgttaatcgg agggaccata aatgaaagta
     3001 gtagtaattg gctgtactca tgcaggtact gcagctgtaa aaagtatttt aaaaaatcat
     3061 ccagaagcag aagtgacagt atatgaaaga aatgacaata tttctttctt atcttgcggt
     3121 atcgcacttt atgtcggcgg agtagtaaag gacccagcag gcttgttcta ctcaaatcct
     3181 gaagaattat cttcattagg agcaacagtt aaaatggaac acgatgtaac agacgtcgat
     3241 acagaaaata aaacagtaac ggcaaaagat ctgaaaacag gagaaacaga aacggtttct
     3301 tatgataaat tagtgatgac aactggttca tggcctatta tcccaccgat tagtggaatc
     3361 gatgcgaaaa atgtactcct atgcaaaaac tacaatcaag caaatgaaat tattgcacaa
     3421 gcgaaagaag caaaacgtgt agtaattgtt ggtggtggat atatcggtat tgaattagta
     3481 gaagcttttg tcgaatctgg aaaacaagtg acattgattg atggattaga tcgcatcttg
     3541 aataaatatc tagataaacc attcacagat atcttagaaa aagaattgac agaccgcggt
     3601 gttactcttg cgctaggcga aaatgttact gaatttatca cagatgaaga aggaaaagtg
     3661 aaacaagtag ctacaccaac agatacattt gatacagata tggtcatttt atgtgtgggc
     3721 ttccgtccaa atacaaaact gatcgaagga aaagtagata cactgccaaa cggggcaatc
     3781 aaagtgaatg aatacatgca gacaagtaat ccagacattt ttgcagctgg agactctgca
     3841 gtagtcaact acaatccaag tggcacgcaa aactatattc ctttggcaac aaatgccgtt
     3901 cgtcaaggtt tgcttgtggg aaataacttg acagatcata aaatggcgta tcgtggtact
     3961 caaggaactt caggacttta cctatttggc tggacgatcg gttcgacagg tgtgaccaaa
     4021 gaaagcgcaa ctttgaacga tctagaagtc caagcgactg tatttgaaga taattatcgt
     4081 ccagagttca tgccaactac tgaaaaagta atgatggagc tagtctatga aaaaggaacg
     4141 aatcgtatcg taggtgctca gttcatgtcc aaatacgaca ttacacaatc agcaaatacc
     4201 atgtctttag ctgttcaaaa taaaatgact gtagaagatc tcgcactttc agatttcttc
     4261 ttccaaccac attttgaccg cccatggaat tacttgaacc tacttgcaca agcagcgcta
     4321 ggcgaaatcg aaactgcaca ataaagaaaa agggttgtga cacaattttg tcacgaccct
     4381 ctgtcttttt ttgtgaaaag tacaattagt tgtctttctt cgaatttttt gtcacaataa
     4441 gcagcaaaga taaagctgtt acataaaaga acaagcagct ttatttgatt ttgaaaagaa
     4501 aggtgagaaa gaagatgaat aaagaatatt ttgtccaaca agatgcattt ttagaagatt
     4561 ataaaagtgc ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct
     4621 ctcagttgaa tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata
     4681 atgtttctca tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat
     4741 tgaatacaga gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt
     4801 aaagacagtt tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta
     4861 ccgatgactg tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca
     4921 acccctttt
//
LOCUS       DAZUKT010000141         4882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000141 DAZUKT010000000
VERSION     DAZUKT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.149"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG816_002885"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG816_002885"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4921699.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG816_002886"
     CDS             complement(1297..2133)
                     /locus_tag="ACG816_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921700.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG816_002887"
     CDS             complement(2317..3534)
                     /locus_tag="ACG816_002887"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4921701.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG816_002888"
     CDS             complement(3683..3844)
                     /locus_tag="ACG816_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921702.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG816_002889"
     tRNA            3917..4000
                     /locus_tag="ACG816_002889"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4882)
                     /locus_tag="ACG816_002890"
                     /pseudo
     CDS             complement(4240..4882)
                     /locus_tag="ACG816_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1496 a         1012 c          783 g         1591 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa at
//
LOCUS       DAZUKT010000142         4872 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000142 DAZUKT010000000
VERSION     DAZUKT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.150"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..684)
                     /locus_tag="ACG816_002891"
     CDS             complement(55..684)
                     /locus_tag="ACG816_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921703.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(681..2057)
                     /locus_tag="ACG816_002892"
     CDS             complement(681..2057)
                     /locus_tag="ACG816_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4921704.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(2137..3399)
                     /locus_tag="ACG816_002893"
     CDS             complement(2137..3399)
                     /locus_tag="ACG816_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921705.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(3412..3759)
                     /locus_tag="ACG816_002894"
     CDS             complement(3412..3759)
                     /locus_tag="ACG816_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921706.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(3779..4648)
                     /locus_tag="ACG816_002895"
     CDS             complement(3779..4648)
                     /locus_tag="ACG816_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4921707.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
BASE COUNT         1317 a          994 c          698 g         1863 t
ORIGIN      
        1 aaagcctgtt ctatttgctt caaaaagttc tagcaatctt tttcacctct actgctactg
       61 ccaaacaaat ttagctttat catgccatgt ttcagacatt acttttttac cattttcgtc
      121 aaatatcacc gcttcccact ttgctgttcc atccggctgt ttacaaatat ctgcaaacca
      181 taggatcgtt tttcgatcgt tgaatttcct tactttcttc tcaaatttat tgatctgaac
      241 tctattttga ttgctgttcc aacctactaa taggcggtta tctgattctg taccatgaaa
      301 accaatggga acgatgtggg ttctatcaaa tgcttctttt ccttctggat agagcattgg
      361 tctaagtttc ctacgtttca agtcatctct tgcttgatac cgccatgccg attttggatt
      421 gaatttaata gctatttgat gcttgctttt gtcatggtaa aaatatgcga catcggcttc
      481 agctccaagc aacttttctc ttgcactatt tgattcaaac ggtttattca cttctaaaac
      541 cttcacccct aaaatgccat gatcaagcat ctgtttagga gttagtaccg cttttttatc
      601 tcttttcttc gctcgtttca caactacttc atctgtatat ttatatggag taagccatct
      661 taaaaaagaa ctgacgatga tcatttatgc acctgttttt ttgcttttaa cattttttgc
      721 atgataggtg aagtacttcc atttatttgt ttcttttcag atttatcagt tgtgatcact
      781 acgttttctg agccttttgg atcattagct ttaggtttgg ttttagatgc aatcggctgt
      841 ttattattcg tgttgtttga ggtttgcggt tcaggatcag cttttcctaa tgcccttaaa
      901 actttttcta gtttttctgc ttgcagtttt tctagtgttt tagctccctg ctcgactttt
      961 tcttctttgt tcttttcaag cttaatagca aacgcactct tcttttcaat tgatggctct
     1021 ttagatgatg gatttgctaa aggtgtgttc aaatttcttt tagcagattt tcttacagga
     1081 gctttaccaa ttccaccaaa atcctcgtct tcaccgccct caccacgagt ttcagctacg
     1141 attgctcggt aatcaacata ttctggttct ggcatagtat ttctaaggtg attgaagatt
     1201 gtttcaattt ctttgttact gatgtaagct ccctgcgctc taaccatact tccatcaaca
     1261 agcttaataa gcatatctcc ctttggactt agactttctc caccgccttc atctaataca
     1321 atcctacttt ctaaatcact tgctaccata aggcaaacac gtgacggaaa attggctttg
     1381 ataataccag tgacaactgt tgatcgagga gattgtgttg ctaagataac atgaatacct
     1441 gctgcccttg ccatttgtcc taattgaaca atcaaacctt ctacttcttt atgtgttcca
     1501 accaactgtg aatattcatc aactaataag atcacatagg gaaatctatc taatccattt
     1561 ttttcggcat attcgttata tagcgtgatg ttttgtaacc ctgctttttc catcagtttg
     1621 taacgttcat gcatcaagat cacaagatat tcaaaaaaat cataagcttc atccatatct
     1681 gtaattggat cagccaacat gaacggcaat tttttgtatc ttccaaattc aacccttttt
     1741 gggtctacaa ttcctatttt taattcatca ggcgttgagt gacacattac tgtaacaaga
     1801 atttcattca gtaatactga tttccctgat cctgtcgttc ctgcaaccaa catgtgtggc
     1861 atttcgctga tgcaaaaatg agcaattgtg ccatcaggct taattccaaa aatagcatct
     1921 gttgggtctt tagcctttgt gttcaacata ttgacactga attttccatt aagctttata
     1981 ttaggttctt tatctagcaa aatagattca gctttttcgg ctctcttttt gactttctct
     2041 aataatttca tagccatttc tattcctcac ttttctcggt acttttctgt ttgtgaactg
     2101 gtagtttgta ttttctgttt ctatttgtga tgtcttctaa aacacgctat tgatcattga
     2161 tggtacatca attggaatac gatcattttc tcctaatggt aaagtaattt gaatacgccc
     2221 tgcgttgatc cgcacgatta cacctgatat atgcaagtaa gtttcaattt cttctttcat
     2281 tgagcttagt tgaatttttc tggtacgaga taaagtaaac aaatattcaa tagatgtatt
     2341 cccaacgtgc atatctgtta gacttgcagg tacgtcagca gaagatagat aaagttctaa
     2401 agattgcttt tgagtttcgg catattttct agctttttcg gtttggtttt ggatattttt
     2461 ggaattatcg gcaaagagat tgattggaaa agaaaattgt gaatttgaaa ctgcgattga
     2521 agatgttgct ctttccgttt ctttgttttt ggtacttact ctttccttac gttctctttc
     2581 aatatttact gatgctcgga aaatgaaata atcgcctgaa acttcatctt ctttttcacc
     2641 aaatttggct cgtccgcttg ttgctcttgt caggatcgac cctaaatctt gaatttgtcg
     2701 taaaactgtt tctctaactt tcttcccttt tggttgcggg atttttacga tatattctcg
     2761 aatatcttca ttgacactta gatcacgtct tgtatgaaca gaaattttga tatttttaag
     2821 cgttgatagc acatcggatt ctaattgcgt tttgtttcgt tttctttttt tgttgttcgt
     2881 ttcatctcct ataatcttgt ttggatcata cttcacatcc atagcttcta gagcttctct
     2941 tttcaacctt ctactttcca tatcatcaaa aaatggtctt tgttctccat agtgatgcct
     3001 gaacatcaaa gtaaccaaat atccaacaaa gaataccgtt cctgcttcta atactaaaat
     3061 agagatgtct accaagtgta gctttgcaaa aaagctttgt acattatatg aataacttaa
     3121 ataattggga aaaaacgcat tgagtaactc tttaaacatc tcttttaatc ctaaatcatc
     3181 tgttccgtta acatcattta acgagacaag aatacttact aaggtatcta caagcagttg
     3241 tacaaccttt gttacccaat gcaaaatata tatgccaatt gcaccaacta ccaatgcacg
     3301 tccaaccaga tatactgatt tagaaaaact ggctacagga taatattttt ctccatcgct
     3361 tcgcttatat tcctttaatt caatggattt ttcgctcatc tgaatatctc cttattttct
     3421 ttctgatttt ggttcatcgt cacttatgct gtcctcacac atctgcgtat ctttaggaat
     3481 gattactctg atttcgtcta acatttctct ctcaatgatc atcgcccctt ttgtactaac
     3541 agttttctct gataattcaa cacatttccc tatttcagaa tctggatcaa aaatagcctg
     3601 ttctacgtca aaatgattct tagctacata cacgatactc attgtttcta actctctagc
     3661 aactgaatat ttacccttct tcagaagata gtagccattt ccttttttta ttcgatttgc
     3721 aactgattta tctaatccat tgatgcttat tgtttccaat tcttcacctt ctcaatttct
     3781 aatctcttac ccaagtacca ttttgatagt tgatgttgat tcctttttga ttgttaaata
     3841 cataaacatt aaggtttacc gtttttccat tatcgttaac tgagaaagct tcgacatgag
     3901 aaccacgagg aactaattca ttccctttat aaattggatc aactttatac aatacttttt
     3961 cattcttatt tttatagatt gcatcacgta caatcgtttc tgagtaagcc atccctccat
     4021 tctgatcatt tttcccaaca ttttgcgctc tcgttccaag tgttaaattg tgtacctcca
     4081 tatctcctcc tagcgaccat gctaatgaat gacttttgtt atagagataa cctttatagc
     4141 cttctagctg agtgatttca ttgtttgaat ctttgccgac ccaagtatct ctctcttgat
     4201 caaatactcc atccttgtat tctcctgcaa tatggacaga acttttgaaa gatggacgtt
     4261 ccacattttt agatgagtga gaatttacag catcataggt gatcaacgct tcaacatctc
     4321 ctgctcttcc aagttgatct aaatctgcat aatttaccca aaaggtttga ctgccttttt
     4381 ctgataatgc ttcatggtct tcttttgtca gtgtatattt cccatcatta atgtttacga
     4441 tattatttgg atatttgttt ggatcataac gtatccctaa aaattctttt gattcactgt
     4501 ctgctttatt acctacctga acactacttt tgatctgaaa atcatgttcc tgagtactgt
     4561 tattctgaaa gtaacccttg ccactaagac caaccgcaac taaaaccaaa attcctaaca
     4621 acgatcggct aaacttcttc tttttcatac caccattcct tttattgtgt tatcataata
     4681 agataaatat ataataaact atattgtaaa ttttgtcaaa tgtattttta tttattccca
     4741 ctataaacct tgatatatag ccaatttaaa aaaggatggc aaaaaattat caagttaaaa
     4801 aaataaattt tgaaccaaat tatttttaat aaaatagcta gtttttttat caaaaaaaat
     4861 agagcttttc ag
//
LOCUS       DAZUKT010000143         4812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000143 DAZUKT010000000
VERSION     DAZUKT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.151"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG816_002896"
     CDS             complement(207..1169)
                     /locus_tag="ACG816_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4921708.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG816_002897"
     CDS             complement(1171..2610)
                     /locus_tag="ACG816_002897"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4921709.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACG816_002898"
     CDS             2795..4741
                     /locus_tag="ACG816_002898"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS4921710.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1535 a          822 c          793 g         1662 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 atgaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg ag
//
LOCUS       DAZUKT010000144         4735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000144 DAZUKT010000000
VERSION     DAZUKT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.152"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..1101)
                     /gene="xerS"
                     /locus_tag="ACG816_002899"
     CDS             complement(16..1101)
                     /gene="xerS"
                     /locus_tag="ACG816_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4921711.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1450..1645
                     /locus_tag="ACG816_002900"
                     /pseudo
     CDS             1450..1645
                     /locus_tag="ACG816_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3177..3695)
                     /locus_tag="ACG816_002901"
     CDS             complement(3177..3695)
                     /locus_tag="ACG816_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS4921712.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4062..4310
                     /locus_tag="ACG816_002902"
     CDS             4062..4310
                     /locus_tag="ACG816_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921713.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1555 a          777 c          718 g         1685 t
ORIGIN      
        1 tattttttac tacatttaca aagcgttcaa agcatcaatt gtagctgcat tgtctatatg
       61 agtatataaa gttgttgtat ttgtacttgt atgtcccaat tgttgggcag ttaataccaa
      121 tgaatttgtc tgttgataga gtctagtagc caaagtatga cgtaatttat gtggactgac
      181 ttggactctg aatccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
      241 acgcttggct tctccttgat aaattgctag aaataaagct ttttcgtttt ctgttacttt
      301 gtatcgttct ttccgaatag ctagataatt agctaaatat tccattgcaa atgctgctat
      361 atttactgcg tctctcttat ttccttttct agttactacc acattattct tttctaaaga
      421 taggtcctct aaattaatat ttacaacctc ggagagtctt aatccagatc ctaaaatcaa
      481 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttgct
      541 caaagttttt ttatattcat tggcaatata atctagataa ttttgtgtat cttcgtctag
      601 aaataatttt ttctcgattg cgtttgcacg ggcagctaac gtagttttat ctgtgatagt
      661 tgctacttga tccataggat tattttctaa gtatggtttt cctaaaggat tattggaact
      721 tttcgaaaga tatttaaata gaacctttaa tgctgtgata tttctattga tagtagaagt
      781 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
      841 ttcctttgtg cgacgtttca aatgagattt gtacaagttt agatcatagg cagttaactg
      901 tcttatcgag gtgattgaag tatcgggaac gatagactca ctcagaagcc attcataaaa
      961 gagcttataa ttttttagat attctaaagt agttcgaggt gattgatctt tatcctcacg
     1021 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
     1081 atatatttct gtatttttca tgttattcat cctttcgata agtaggggag aggagtatct
     1141 ctataattgg actataccaa ttatagagtg ttttgtcaat tggtatactc tgaaatttat
     1201 taatttattt caaaaaagca gataaatagg tatagattta ataatttaaa ataaatggta
     1261 tacatgaata ggaaaggaaa cagaaggagg caatcttaat tttttaaatt gtctaaatta
     1321 tatagaaaga acaatctttc tatataatta gacataagag agactacggt tgatttgata
     1381 ttaacaagcg tatatgaatg tatatacaaa taaaaaaggg gagggagaag ttaaagaaaa
     1441 ttcagagaag tggggcactc agtagtatta acaatttaaa aggaatttct taaagaaaat
     1501 gggttgcatc ctggagatac aattttttta gataaagaaa aaattcaagc aacaattcaa
     1561 aaaaatctta actgctgaga ttgatgtagc aatatctcaa tcatcaacaa agtacgatcc
     1621 tgcctttcaa aagcttattg ataaatgttt attcattata aaaataaaag aaaaatttat
     1681 gtcagctttt ttcatcaaag tggtaatgat aattcgtcta ttaattgtat tttttaggaa
     1741 atatggtata attgttttaa cgaaaaggta attcatgata caccaaaaac cactagctgg
     1801 cgggctagtg gttttctttt acgttctatt cagattaacg ggtaggctta ctgtcttttt
     1861 tggcaataat tttgcaatca agccatttac agatgtaatg cgcggatact tctgctaaga
     1921 ttgcaaaaaa catatcgaaa aggtaattca taatacaacc tcctcccctt gccataatag
     1981 gccggggtag cagacagcat aaatattata ccataactga aaatttcatt ttagtacatt
     2041 tcaatatatt gatgaaccag cacaataaaa catgccatga ctaacagaaa aaaacttatt
     2101 aaaccagata taacttgtaa acatccaact atatagaaaa cgtaaatttt ctatatagtt
     2161 ggattaagat aacgactatt tattttatca tatttttact ataccctata cttttacttg
     2221 gtatatttat gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat
     2281 aaaaatacta aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt
     2341 atttagataa cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct
     2401 actcaatcga aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag
     2461 accttttaca tcttgcagaa atcgttactg ggattaaact aagcaataaa aactaatgtt
     2521 cccctcattc tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac
     2581 cttcaggatc ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag
     2641 gataggcgat aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg
     2701 tatgttggct ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa
     2761 tcgttcttaa caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt
     2821 gccttaccct tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc
     2881 ggcggtctga aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc
     2941 ctttgtagcg gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt
     3001 tggccaacca aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc
     3061 acttgcattc acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct
     3121 tactgttaac atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac
     3181 aaagctaatc tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta
     3241 tttacatgaa taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat
     3301 tttcttttgt gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata
     3361 tcatcaatat tacgattgag aatttcctct aattctttca atggtagatg tgcataggta
     3421 tttgtaaacc acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt
     3481 ttaacagata atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca
     3541 acttgatatg ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc
     3601 aattcttcag gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct
     3661 ttttttaatt cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat
     3721 tgtctccttt aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat
     3781 atctgatatg cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc
     3841 tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg
     3901 tagtaaaatc ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact
     3961 actatcaatt actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa
     4021 gtgaggatag ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc
     4081 tttatatagg attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg
     4141 aagtaaaaat tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac
     4201 gaaatgatca actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg
     4261 ctcgacgaac tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag
     4321 ctttttatgt tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc
     4381 tagggtgcgg tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta
     4441 aaattatgaa taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct
     4501 ggtgttatag attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa
     4561 accgatactc tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa
     4621 acttcactaa cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt
     4681 atcaaaaatg ctgctcattt caacttatat gttttagtga actaccccca cttag
//
LOCUS       DAZUKT010000145         4731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000145 DAZUKT010000000
VERSION     DAZUKT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.153"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1013)
                     /locus_tag="ACG816_002903"
     CDS             complement(15..1013)
                     /locus_tag="ACG816_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4921714.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1088..1384)
                     /locus_tag="ACG816_002904"
     CDS             complement(1088..1384)
                     /locus_tag="ACG816_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921715.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1377..1670)
                     /locus_tag="ACG816_002905"
     CDS             complement(1377..1670)
                     /locus_tag="ACG816_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFS4921716.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1816..2367)
                     /locus_tag="ACG816_002906"
     CDS             complement(1816..2367)
                     /locus_tag="ACG816_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS4921717.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2541..2840)
                     /locus_tag="ACG816_002907"
     CDS             complement(2541..2840)
                     /locus_tag="ACG816_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS4921718.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2867..3142)
                     /locus_tag="ACG816_002908"
                     /pseudo
     CDS             complement(2867..3142)
                     /locus_tag="ACG816_002908"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            3480..4163
                     /locus_tag="ACG816_002909"
     CDS             3480..4163
                     /locus_tag="ACG816_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4921719.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4200..4697)
                     /locus_tag="ACG816_002910"
     CDS             complement(4200..4697)
                     /locus_tag="ACG816_002910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4921720.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1402 a          907 c          693 g         1729 t
ORIGIN      
        1 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
       61 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      121 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      181 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      241 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      301 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      361 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      421 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      481 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      541 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      601 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      661 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      721 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      781 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      841 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      901 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
      961 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1021 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1081 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1141 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1201 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1261 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1321 gcatatattg atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1381 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1441 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1501 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1561 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1621 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1681 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1741 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1801 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1861 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1921 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     1981 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2041 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2101 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2161 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2221 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2281 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2341 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2401 acttcaatta ccattgccgt tataagtgtc aacactagtt gaaaatattt catattcttt
     2461 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2521 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2581 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2641 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2701 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2761 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2821 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2881 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     2941 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3001 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3061 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3121 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3181 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3241 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3301 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3361 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3421 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3481 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3541 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3601 aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3661 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3721 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3781 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3841 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3901 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     3961 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4021 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4081 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4141 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4201 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4261 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4321 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4381 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4441 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4501 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4561 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4621 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4681 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa t
//
LOCUS       DAZUKT010000146         4612 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000146 DAZUKT010000000
VERSION     DAZUKT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.154"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            961..1563
                     /locus_tag="ACG816_002911"
     CDS             961..1563
                     /locus_tag="ACG816_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFS4921721.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            1918..2154
                     /locus_tag="ACG816_002912"
     CDS             1918..2154
                     /locus_tag="ACG816_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFS4921722.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2151..2564
                     /locus_tag="ACG816_002913"
     CDS             2151..2564
                     /locus_tag="ACG816_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4921723.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3107..4033
                     /locus_tag="ACG816_002914"
     CDS             3107..4033
                     /locus_tag="ACG816_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFS4921724.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4030..>4612)
                     /locus_tag="ACG816_002915"
     CDS             complement(4030..>4612)
                     /locus_tag="ACG816_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4921725.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1666 a          623 c          812 g         1511 t
ORIGIN      
        1 ttttagttac tgtttttatg acacatgaac acacttattc ttatttcctc ttatctgcta
       61 tcaacagaaa agaaaaagcc ctgtcaagag ccgattagat caagccagtc gagaaacgat
      121 gttagaccaa attgattttg ggtaatgaat gtttagaact tagtagtcat ccaaaaacga
      181 ctactttttt tgttgaatcg tccaaggacg attcgatgct tttgacttgt aagaacagga
      241 ttgaatgaaa ccatactgtt cgcatatttt tagtggatga acaaacaaaa tacgagagat
      301 tttttgttcg ttcatccatg gttttagaaa aaagagggac gattttggaa gaagaaaatc
      361 gtctcttttt tttcttcttt ttgtatgaca aaaagaaaga tcttttgccc attttatttt
      421 tataaaatgg gtaggtggcg ttcgcgtaaa gcaaatcgat acaatccaat ggttttggcg
      481 aatcagattt accattctag gcttgtttaa tacagacatc gattgttatt ttgagaattt
      541 atatccttat tggcaatgaa atggatattc tccataccaa tagaaatctt caagataatt
      601 ttcggaaata tataagagtt aatcaattat gatcatatag taggtaaaaa ttaggacagc
      661 cttcaattat atgggaaacc aatataatca agtaattatt cgggtaacac aaatactaga
      721 gtactcattt cttataactg ttttacacct attagggatc agtattatta atagatctag
      781 ataaattaat acgtaattat ataataattt atgtttgtgc cattaaacaa tcttaacaga
      841 tatttcattt tatttatgta aaacaacacg aataattatt gcttcgtaaa gttatatgag
      901 tataagttaa tttactggag gtagtgcaat gcttttttct gaaagatttg gttttaaaaa
      961 atggaggata tcatactgag agatagattg acagaccctc ttaaaaatag tttagcaaat
     1021 gtgatttatg atatattacg agaggctgat aaagtttata gtaattataa tggaaaagtc
     1081 tcatgggtaa tatattcttt tttaattaaa gagtttttta aagaacagat aaataatagc
     1141 tatgagttgg gttatcattt tactgtttat tatgaagata ttatcaagag gttagaagac
     1201 ttggagtggt atgcttatta tgatttatta gaggtttttg caagtgctaa aatcttggat
     1261 aaaaagaaaa gagaggaagt aaataaaatt ctggagtcgg aaaattctgg ttacagatta
     1321 agcgaaagca atgaatttat tccaatcaca gatgatattt ctattaagtc tataaatgct
     1381 gtatctgaat caccatttaa ttacgcaaaa aatcatatcc ataaatctct tacatatata
     1441 agcgagaagg aaaatcaaga ttataatact gtagtgagag aagcaattag tgctgtcgaa
     1501 agttgtctta tcgaattttc aggattacca gctaataaaa aaatactttg ggaactgcta
     1561 taaaagcaat taaagacaaa tcgaataaca atgtcgatct tacattttta aaaccatttg
     1621 agacaatgta tggtttggcg agtaataatg gaatacgaca tgcagaaaat gaaaaaacaa
     1681 ttgtctcaga tttacctgat gcagttttgg tgttaacaac atgttcagcc gtaattaatt
     1741 atttgggagt aaaaatagca tatgactcca ataaatcatg aaaagttaaa attcatccaa
     1801 gagctacatt ataactatta atttccaact ctttaaaagt ttagtaattg aaggaagtaa
     1861 taagggtgat tgaacaatac ggatatacgg tgtatactta tcgtaggagt tgatgatatg
     1921 aatattaaaa aacgcaaaat cagagaagta ggtaattcaa tagttgtaac tttgtcaaaa
     1981 gagagtttat tgcaaaaagg tttgaaacct ggagatacta tttttattga tgaagataaa
     2041 atgatggaag ctattgtaaa agaggaaagc aagatagatc ttgaaattga tatgtatgtt
     2101 aaccaagctt tatctgagta tgatgcagct ttcaaggaat tagttgaaaa atgatctatc
     2161 taagtgaaaa agatattgta aaattgaatg ttattcaaat aaagaaatat tcaccgcgag
     2221 aaacaatcgg agtcattgac aaaaatgcgc tgagtatggc tgtaaatcaa cctaaacagc
     2281 atatttttgg aaaagatctg tatcctgata aatttagcaa agcagctgtt ctgctaataa
     2341 atctgatcaa aaagcatcca tttcataatg ctaacaaacg tacagcattt ttagccgcgt
     2401 atgtttttct caagctaaat ggctattcac taaaaatgaa aaatcaggaa gtagtagaat
     2461 ttgtagttga aattgctacg tatcagggtg attttgatga cttaaaagag gcaacaacag
     2521 atatactaaa aaagaattca acaaagaccg attcaaaaaa gtagaagcaa actagggatt
     2581 cagttaaatc tgaagattta gttttttctt tattttagtt tacataagat acattatgcg
     2641 aagttcatta ttttaaaagc ttaaataata aactttttta taataccaat ttatttatgt
     2701 aaaccaatga ccttaatcgc ttaagcaagc atgttatgta atactctact ctctccaaaa
     2761 ctaatgacta tctattttca aagaagctag aatgattatc tgatcaaatg ataatttata
     2821 tttaattcct ggtctactgc tcttctttta atcaattaat gtctaacgta ctgtacacga
     2881 taaagtagga agacttattt tcaacttcgg attcattttt ttaattcggt gaaaatcact
     2941 aaattaagag tttatccaag ttcattttag ctgagaatac gcttgaaaat aattttgatg
     3001 aattttcctg aattaaaatt tattaacttg ttgagtgata taattgtaat gtattaccaa
     3061 atagttagca cttagattag tgatggaaag gaatagtgag aacaagatga ctggtagaaa
     3121 tcccaaagta tttatatcat attcatggga gaataaggaa aataaagaat gggctagaga
     3181 actagcagac aaattacttg aaaacggtat tgatgcatat atagatcagt atgatttaag
     3241 tttaggtgat agattacctc agtttatgga gcagcaaata tccgattcag attatgttct
     3301 aattatatgt acaccagagt ataaaaaaaa ggcaaataat cgtacaagtg gtgttgggta
     3361 tgaaggtcat attatctcag cagaattgct ttctggaaaa aatgaacgta aatttatccc
     3421 aataattaaa gaaggacgtg caagcgaagt acttcctact tttcttgctg gaaagcttgg
     3481 aattgattta tcagatagcc agcattatga tgaaaactta gatgatttaa ttacaactat
     3541 atttggtgtt aagagaaaac cagcattagg tgatatacct aggcatatta aaggcagtcc
     3601 tttactacct atagaacata atgaaaataa agatattgaa atcttaggaa ttatcactga
     3661 taaagtaact gtacctaaaa tggatggctc aaggggttct gcattatata aaattccatt
     3721 tagattaaat agagtaccaa gtcagctttg gaaagagttg tttgttagaa attggaattc
     3781 accaccacga tatacaacaa tgcatagacc aggaattgct tctgttgtag acgatgaaat
     3841 tttacttaat ggaacaacta ttgaggaagt ccgggaatat catcgggaaa ctttaaaact
     3901 ttgcattgaa aaaagtaata ctcaagaaaa aaaaatattg ctagaggagg aacaaaaaaa
     3961 agagagagag aatgagcgag tcagatcaca taaagaaagt gttcagaaat tagcagatga
     4021 attagagttc tagttgatag tcagttcttt cttaattcga tacacagttt gtctagttac
     4081 attatagtct gttgctattt ttgaaatagc aacaccattc tcctggtcct gaacgatgct
     4141 tctataaaca aggcgacgtt gaggatcttt tgtgttcgca ctgtataact taggacgtcc
     4201 tttatagacg ccgtttgctt tagcaatctt tatcccttgt gcctggcgtc gttttgattc
     4261 agtacgctct tgttcagcaa tcatggccaa gatttgaatg attaggtctt tgataaattt
     4321 atccaggaga ggatttccga ttgcttcagc catgatcggt aaattagtga taataagtcg
     4381 cacatttttc ttctttagat aatttactga atcgataatc tcgtcataat tacgtccaag
     4441 tcgatcgata gcctctacaa taaaaatatc ttggtctcta acaaaatcta gaacttcctg
     4501 aaaaatcggt cgatgagtaa tagtcgctcc agattgtttc tcgatgaaaa ttttttcagc
     4561 accaaatttt ctcatttcct cgatttgacg ttgttcattt tgatcgatcg at
//
LOCUS       DAZUKT010000147         4592 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000147 DAZUKT010000000
VERSION     DAZUKT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.155"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..2857
                     /locus_tag="ACG816_002916"
     CDS             188..2857
                     /locus_tag="ACG816_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4921726.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2948..3562)
                     /locus_tag="ACG816_002917"
     CDS             complement(2948..3562)
                     /locus_tag="ACG816_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4921727.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3682..4221
                     /locus_tag="ACG816_002918"
     CDS             3682..4221
                     /locus_tag="ACG816_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921728.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4218..4556
                     /locus_tag="ACG816_002919"
     CDS             4218..4556
                     /locus_tag="ACG816_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFS4921729.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1422 a          790 c          860 g         1520 t
ORIGIN      
        1 aaatttttaa gggtgggact tttgattcct accctttttt tgcgtttatt tttgttatac
       61 gaacaaatta tgaagttatt tattctaaat tttatttttt tgtgaaaatt agactctttt
      121 taagcagaga aaaacaagga tatgataaac tgaatacaac ttcatagaaa tttggtggaa
      181 aataaatatg aaaaaaaatt taaagtcatt ctttagagaa aacggacgct acgcagcagc
      241 tagtttcctg attccgtttt tgattttagc acttgtttat ctaagtatcg ggatctatcc
      301 tggaagcagc agaagtattt tggcaagtga tgccttttca cagttttcta attttcatgc
      361 cagcttccga aatgttcttc ttggaaagca aagtattttt tatacatgga atgcttctct
      421 aggattgaat tatttatctt taatttctta ctatctaggc gggatcttca cgccactggt
      481 gttctttttt ccaaaccaga tgatgccaga tgcactgtat gttctgactt tgcttaaggt
      541 cggctgtgcc ggattggctt tttggtttct tgcacgtacg ttcaagattc ctcgctgggg
      601 gcatgttgca cttagcgtca gttacgcatt gatctcattt atcactgcac attcggagat
      661 catcatgtgg ctggatgcat ttgtttatct tccattagtg attttgggga ttcatcgtgt
      721 tatggatctt aaacggccga aacttttatt tatcagttat ttactcttgt ttttatccag
      781 tttttatatg ggttttatga ttggcgtatt ttctttctta tattttatcg cccgtctatt
      841 gacaaactgg tcagaatata aaaaaacgat tgttccttat ggtatcactt ctttactggc
      901 aggcggcgct tcaatgatca ttgtattgcc agcagtgctg gatcttcggt caaatggcga
      961 ggcactgagt cagatcacta cgttgaaaac agaagcgact tcttatctgg atctaatcat
     1021 gaaaaatatg attggtgttt atgacacgac aaaatacgga tcgattccgt tcgtttacgc
     1081 aggtctgcta ccattagtcc tctgtctgtt ttattttgcc agtcggaaag taccgttgaa
     1141 aaataaattt ttgtacggca gtcttttcgc catacttatc gcaagtttct atcttgttcc
     1201 attgaatttg ttctggcatg gtatgcatgc accgaatatg tttttattcc gttatgccta
     1261 tctgttctct ttcttagtgc tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga
     1321 cgatcgtggg ctgttgttag gaatcatttt ggtcgtagct gctctctttg cggtggcatg
     1381 gggcatcaaa cctgaaggaa gctacaccta tgtgacgatg acttcctttg tattgacagt
     1441 ggttttctta gggatttatg cttttgctgt gggattttat cagcataaaa aactttcatt
     1501 caatcatctg agtatattgt tattattgat catgtctgct gaagcactgg tcaatacgaa
     1561 cagtatgatc catggcattc ttgatgattg gaattatgct tcccgcagtt tatatactga
     1621 tccttatcct gacttgaaag ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta
     1681 ccgcttggaa aatctggatc cagtatctgc aaacgattcg atcaactatg gttacagtgg
     1741 tatcagtttg ttttcttcta taagaaatcg taattcttcc tcttacctgg ataaattagg
     1801 tttccgttca agagggacaa atttgaatat ccgttataac aataatactt tgctaatgga
     1861 tgggtttaca ggaatcaaat ataacatttc taaaaacgaa agcacgttca gtaaatatgg
     1921 cttccgccaa gctggtcaaa gtggcgacta tacactgtat cagaatgtca atgcattgcc
     1981 actagcattt actgcaccgt tgtccgttaa tgatgttgag cagccagtga cagataattt
     2041 gaccagtcag acaaacttga tcaatacatt gtctggttta aatgaacagt attttacatt
     2101 ctatacacct actttgaaga atcagttgaa tacgacgatc acaaagacaa cgactggcgt
     2161 cacttactca gaaacagcag ataaccagcc aaaagagctc acttgggaag tccatgtgcc
     2221 tgcaaataca caagcttact taagtttgtt cccaaccaat tttgcacaat tagaaagttc
     2281 tagtgcgacg atcactgtca atggtacatc gcgaaaaacg caaatcaaca tttctggtca
     2341 atactatgat cttggctatt atcctcaaga gacaacagtg acatttactg caagttttta
     2401 tggaacgaaa gaagtaagtt tcatggagcc taaagtcgtt ggtctagatg ttgtagccta
     2461 tcaagccgca atgaatcaga tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa
     2521 agcttctggg actgtaaata ccgctgaaga ttccatgttg gtgacaacta ttccttatga
     2581 caaaggatgg aaagctaaaa ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc
     2641 attcttgatg ataaaagttc cagcaggtaa acatactgtc actttcagtt acttgcctga
     2701 aggatttacg ataggtgcaa tcctttttgt cctatgtatt gcattgttta ttctttatgt
     2761 ctggtacctt gaccgcaatc gcccctttta tggcacgatt gatccagcaa tagaagaaaa
     2821 accgcgaaat acacacagaa gaagacgaag aaaataaaga aacgatggaa tctgtgtcac
     2881 tcttctattt aaatggcaaa aatttcaatc tttaaatgac aaaaaccacc tgatttttgg
     2941 tggtttttta tatacctatg actaaactat ctcttgctgc ttcgacaact tctacagtga
     3001 tcttatccat ttgattgatt cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa
     3061 gcctaaaatt tccttttgtg atcttaatga tagttgtgat agcctcataa tcagaaaaat
     3121 cttctagctt gatatcaatt cctaaatctg cccatttgta ttccaaaata tgatgtgttt
     3181 cgtccttact aaggttatca aattcatgtg caaatcctat gcgagagtaa agctgcggat
     3241 aacgagacaa tcttttttca ataccaggca taccgataag aaccattgcc agatcatttc
     3301 tatcgtaaat atctcttagc tgttctaaat gctgaagctt taaacgatca atttcatcga
     3361 caataattaa gtcaattgct tcataggttt taggcacagg aggatcttct tgattgtatt
     3421 ttagtaccat atgcattgct ttagtcagac ctattctact agttattgtg ttaatatccg
     3481 aactgacacg agacatcttt tcagctggta cagtataaaa aactttattt acacttaata
     3541 tttcatctgt tgcatttatc aagaacaaga aaaagtctga atattcaaaa aaaataacgg
     3601 tgtacatttt taaaaggaga tgtataatcg agatttagaa agcgttttcc tcaaaattta
     3661 atgaattagg aagtgattac tatgaaaaaa ttatttagcg cactttttat tctgttattt
     3721 ggctttattg gtattgggac ttctggaaat atagttaaag ctagcacgat ggttgaacaa
     3781 acaagtgaaa tggcaaaaac ttttagtcaa ttaacaccag aagaacaaag ttacttcaaa
     3841 tccaaaggat ttagtgataa agatactttt tacactcaaa agatagagcc tcaagatccg
     3901 aaaacacgtt tttctgcaaa tgtattgcgg ttaaccgcat ctactaaaaa aattactaat
     3961 acgcaggggc ggacagaata tattataaca agtcccaatt cggcgatgta tcgtatttct
     4021 acacgcttga gtttaggtag tgcaaaatca tatactcaaa cagtaaatct agctaattct
     4081 tatggttatg gtggaggaat gtattttaca tttactggga aaagacaata ttacagtgta
     4141 aaattagcta tgcaagttta taatacattt ggtgtagggt cagtctcttg cactgcggga
     4201 ggattgacaa taggaaaatg ataatttctt actatgtctt aaatagaagt gatgatgaag
     4261 gactcttcgt ttttcaatca ttacatgagg atacgatgct aaaagagttt tataatcgga
     4321 ttaagaataa acagatagaa acaaatttat attttttaag tgactctatc ttgcctttta
     4381 ttagcaaccc taaaattctt aacttgttgc agacaaaaga tttgaaaagt ttaccgatta
     4441 ttgtcattga tgaggaaatt gtaaaatatg gcgcttatct aagcgtagaa gaattagaaa
     4501 aattaactaa cattggaatg agtattcagg aggataatgg ggtacatgga acatagaatg
     4561 aatatgcaca gttgagaacg gaatttgaca tt
//
LOCUS       DAZUKT010000148         4548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000148 DAZUKT010000000
VERSION     DAZUKT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.156"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1152
                     /locus_tag="ACG816_002920"
     CDS             82..1152
                     /locus_tag="ACG816_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4921730.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            complement(1118..1204)
                     /locus_tag="ACG816_002921"
                     /pseudo
     CDS             complement(1118..1204)
                     /locus_tag="ACG816_002921"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1248..1709)
                     /locus_tag="ACG816_002922"
     CDS             complement(1248..1709)
                     /locus_tag="ACG816_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFS4921731.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(1969..2517)
                     /locus_tag="ACG816_002923"
     CDS             complement(1969..2517)
                     /locus_tag="ACG816_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921732.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2518..3375)
                     /locus_tag="ACG816_002924"
     CDS             complement(2518..3375)
                     /locus_tag="ACG816_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4921733.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(3661..3948)
                     /locus_tag="ACG816_002925"
     CDS             complement(3661..3948)
                     /locus_tag="ACG816_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4921734.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3938..4267)
                     /locus_tag="ACG816_002926"
     CDS             complement(3938..4267)
                     /locus_tag="ACG816_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4921735.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4344..4490)
                     /locus_tag="ACG816_002927"
                     /pseudo
     CDS             complement(4344..4490)
                     /locus_tag="ACG816_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         1379 a          877 c          658 g         1634 t
ORIGIN      
        1 aagaggacag catatcgata ttgcttcctt ttttttgcta tttatttaag ggatagaagt
       61 ttagaaagga ctgtttataa gatgaaaaat gatatagtga cttatgcaaa cacaaaatgt
      121 gaagaaattg gcatcagtaa tttagaagag tgcttttttg acacaaatga cctgtcgcaa
      181 tttttaagaa cgggaagaac aaatgccagt aaacttgcca atgacttagt tgcaaagaac
      241 gttctattta aaatcaatac tcgtccagtg ttatttgcca tcaaagagcg ctttgaagag
      301 cactttttga ttccggtaaa agatgtatac gaaagtatat ctgaattaaa aacagacttg
      361 gagcccaatt ctacagattc cattctacaa aagatgattg ggtatgatat gagcttatcc
      421 gatgcggtgg aacaaatgaa aactgctgtc ttctatcctg gaagagggct tcctatgatg
      481 atcactggag atactggagt tggaaaaagt ttttttgttc atactgctta tcagtttatg
      541 aaaaaagtga acgttattca tgagaatgct ccttttaaag tacttaattg tgcgcaatac
      601 cataataatc ctgaattatt gtcggggata ctatttggct atgttaaagg tgcttttacc
      661 ggcgcagatg aagatcgaaa tggattgttg caagatgcag acggtggagt actgttttta
      721 gacgaagtac acagattgcc tcctgaaggg caagagaaac tatttgttta tatggatggc
      781 ggaacttatt ctagagtcgg tgaaactatc catcacgcct ccaatgtacg tttagttttt
      841 gcgactacag aaggcaaaga ggattttctg gaaaccttta tgcgaagaat ccctatacat
      901 atccatatcc caaatattga ggaacgaggt attttagaaa aaaacaaatt atcgaaaata
      961 ctctctttag cgaaagcaaa aactttagca aggctattga tgtttctcct aaagtcacta
     1021 gtgttgcata aatatagtca gaatattctg aattattatt ttcttgaaaa aagtaaaaaa
     1081 aatcgtatcc caaactttca tctacatttc cagaaagatc ttcatgttga taatcaattc
     1141 ctgaatcaat gaccgctacc ttcacatcct tagaaccctt ggtttcttcc caagcggatg
     1201 gcatgctaat ctctcttaaa ttagcttgtt tactccaatt aggatcgtta tcgatttcct
     1261 aaagaatcag ttgtatttgg ctcctcaaca tcattcaacg gctctagttc aaccacatag
     1321 tttggctctg cataattgac catatcagaa gcattaaatg ttcttactgc ctcttccaca
     1381 ctaatttctt cgttcaattt cactaataat acttgatctc gcttattata atcgctttca
     1441 tttgtcctaa gagtcaaatc tttttccacc ataactttta cacccaataa attttgcggt
     1501 ttatttttct ctgttgaaat tctttgtgta gcctttaaac tgacaattag ctcattgggt
     1561 acatactcta tttgagagtc atctaattgg ctcataatcc tttctttttg gttagtatct
     1621 gcagttactt gtttagtatg agccatgaac ccaaacatac tcaccccaac aaccaatcct
     1681 aaatatattt tctttttaaa ctttttcatc tacatctcct cctaatagtt ttattttaga
     1741 gttagctcta ttacttattt actaactcaa caacagatta tcatacactt attaatgaaa
     1801 tcacaatatc tcattaaaac acaccctaaa atcataaccg ttcctatgct cattttttgg
     1861 attttttaat cacgtaacca gaaaaataaa tcccctctat ttcacgcaaa tcaaagaaga
     1921 actaagtaac taccgtttaa aggtagttac ttagttcttc tttgattatt atctaacttt
     1981 attattcatt ccccaccctt tgcgcagtga gtcttcggta tcttgtttta tctgactggt
     2041 agttcttatt atttctccac gatcatctaa ttctggaaca attggatatt tttctctgaa
     2101 caagtctagt tctttcacca cgtcattaaa caaatcttgt ttatcttctt gcgtaagatt
     2161 ctgtttaata tactcatcta aagcttttct aagcaattca tctcttgtca tattagtctc
     2221 atacttttct actaaaactc tggaagtcgc atttaacaaa gtcgacagtt ttaaaggatg
     2281 cttggtggaa taaaccttat caaactttct gattggtgcg ccaacttttt ttgttcttcc
     2341 tctactctgt ctttcaacat tggaggaagt ttttatttct tgaacttggt gcacttctaa
     2401 ttgcttattt tcagatggtg taattgcttg ctgctttttc tttgcttctg cttctttttt
     2461 tattttggcg agtcctttgt tgttcccaaa aatatcaagt cctttgatgt cagccaatta
     2521 ctccacctcc acataccaat tctcgtgttc ttccaattct ttcaaaatac tgataaataa
     2581 ttcgtggctt ttgcgatccc attgttttaa gagacctttc tttgtcttct ccatcgtaat
     2641 acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt acaacatttt taatcaaatg
     2701 ttccccataa atctcttggg cttgtttata catttcctga tctactgcgt caccgctttc
     2761 caacatgaaa ggaataacac cagcaacata taaatttgta tcaaattctt tcacaaaaaa
     2821 gtccatatac tcaagatatt gcatcgcatt tctcatcgat tttacttggg tctgtaagac
     2881 gacgattgcc caatccgcaa tgtacatcgc ggatttacta tatacagaaa tagaaggtgg
     2941 gacatctaaa tagatgacgt caaactcatc aaataatggt tctagctttt ctttcatata
     3001 tttgaattgg gataatgaat cattatattg gtctttgatc caatcatcat acatttcaaa
     3061 ggacatgtct gctggaagta tatagaggtt attgattact ttatagacag cctctttagg
     3121 tggaagattg tttatcaaca cgtcagtcac agtattttcg taaacaatcg gttcaccttc
     3181 tccgcctcct tcttcgttag tgaagttcat atgcgttaag ttcatcacat cagaagtatt
     3241 tccttgcata tccaagtcaa ttatcaaggt tctttttcct tcattaactg ctgattcaaa
     3301 ccctaacatt tgcgcgcacg tacttttacc tacgccacct ttaaaattac cgattacata
     3361 tttcttcgct accacgttcc tacctctttt cttttagcaa tattcatttg atactattat
     3421 aacatattta tttttagtta ctaccaaaaa aaagatatat atgtactacc ttataattaa
     3481 taagtaaaag gtagtacata aataaggaaa agctgaaata tattgattta tagctttcct
     3541 caaagtaatg acgttattta atttatcgag aagcagagaa tactcgcgac tttagtcatg
     3601 tagacgaatc agattttcga taaaaaagaa gccgtccttc ttaagaacga caacaaatca
     3661 ctagattaat tttttcaact gttcataaaa gttttcatgc gttccacata acaatactgg
     3721 aataaccttg ttttctataa tctcgtaagc tactctatat gttgtgcccg catatttgaa
     3781 cccactagac caaatacctg ataaatcccc agttttttgt tcacccttat atggatcaat
     3841 cgcaatttca tcataaatta aattcaaaaa tttttctttc aatggcttct cttttagctt
     3901 ttttaaatac ttaatgacat gttttgaagg aacgatttca taagccaatt tctgttgcca
     3961 actcctcttt agtcattact ttagaattag taagtgtatc ttcagcaata tctctaaagg
     4021 aagaatgcat tttgtttttt ctattcttaa attctacaag aatctcatct ttatcgtagc
     4081 cttctgcaat tatatcagac aaaatatctt cgctaaaatc ataaaattct ttttgtggtt
     4141 caacaaattc ataaagaata ccattctcta ccaactttgc ttcaacttcg actccattag
     4201 gaacattaaa atctttaggt actgtcaaca tgattgaatt gccttgtttt cttgtttttg
     4261 taatcattta aatccctcat ttctaacagt atgtaattac ttagtaaata cattatacca
     4321 cgagataatg tatttacaag taattactgt gactcgtaat gcgtccttgc tgaataaatt
     4381 tcaaccacgt gttcatcttc atctacctta taaacaaccc ggtgttgtct attcaatctt
     4441 ctaaaatacc taccttcatg tgtaggtttt aacttctcaa atgattgggt tctgttgcaa
     4501 agttttaaat aaagaataaa atcccttacg gtatctatga tttaagct
//
LOCUS       DAZUKT010000149         4477 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000149 DAZUKT010000000
VERSION     DAZUKT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.157"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            598..744
                     /locus_tag="ACG816_002928"
                     /pseudo
     CDS             598..744
                     /locus_tag="ACG816_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(832..1125)
                     /locus_tag="ACG816_002929"
     CDS             complement(832..1125)
                     /locus_tag="ACG816_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4921736.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1162..1347
                     /locus_tag="ACG816_002930"
     CDS             1162..1347
                     /locus_tag="ACG816_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921737.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1528..2706)
                     /locus_tag="ACG816_002931"
     CDS             complement(1528..2706)
                     /locus_tag="ACG816_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4921738.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="ACG816_002932"
     CDS             complement(2810..3124)
                     /locus_tag="ACG816_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921739.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="ACG816_002933"
     CDS             3231..3446
                     /locus_tag="ACG816_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921740.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="ACG816_002934"
     CDS             complement(3746..3988)
                     /locus_tag="ACG816_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921741.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="ACG816_002935"
     CDS             complement(3975..4403)
                     /locus_tag="ACG816_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4921742.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DAZUKT010000150         4461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000150 DAZUKT010000000
VERSION     DAZUKT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.158"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1932
                     /locus_tag="ACG816_002936"
     CDS             88..1932
                     /locus_tag="ACG816_002936"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4921743.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            1988..3454
                     /locus_tag="ACG816_002937"
     CDS             1988..3454
                     /locus_tag="ACG816_002937"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4921744.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3491..4333
                     /locus_tag="ACG816_002938"
     CDS             3491..4333
                     /locus_tag="ACG816_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4921745.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1434 a          729 c          969 g         1329 t
ORIGIN      
        1 ttatttagtt tctgcctgtt ttttgtgttg aggatggcca cttcgactca aaagttcatc
       61 actcaaaaaa gaaaagaggg aaacaccatg aattacaaac aaacagcaga agaagtattg
      121 cagtacatcg gtggggaaga aaacatatcc catttggagc attgctcaac tcgtttacgt
      181 tttacgttaa aagacaacag caaggtaaat atgagccagt tagaaaaagt ggatggcgtt
      241 atgggcgttc gccaaaatgt gcaatgccaa gtaatcattg gaaacgatgt tgttgaagtg
      301 tatgatgaag tgaaagcgtt attaggtgac aatgtttcaa atactgaaga agcaccaaaa
      361 caaaaacaaa aattaggttc agttctatta gattttatta tttctatttt ccaaccttta
      421 attcctgcga ttgcaggtgg aggagtattg aagtccttgt tgctactagc ttctatctta
      481 gggctgatta gtgaccaatc tcaaacctat cagattttaa atttggtcgg cggggcaccg
      541 ctttatttct tgcctattct ggtggcaatg actacggcta ataaattgaa agtcaatcaa
      601 ttggttgcag tatctgctgt cggtgcattg atccttcctg aaatgacagc tatgttggcg
      661 gaaggtgcta cattcttatc ttttggattg gataatatag cgtatgcatc tcaagtattc
      721 ccagccatct taactgtttt attttatgct caacttgaaa aatggtttac aaaagtttca
      781 ccaaaaccaa ttcgtatttt ctttgtacct atgatgagct tattgattac agttcctatt
      841 gctttgttgc ttctaggacc attaggttat aatgtaggaa cagctttctc ttctatcatt
      901 gtgtctttgt acaatcaatt tggatgggtt gcaaccggag tcttagcagc tgtactgcca
      961 ttaatggttg taacgggtat gcataaagca atgatccctt acgctgtttc atccatgagt
     1021 gaattgggtg cagaacttct ttatctcccg gcttcattgg cacacaatat ttcagaagct
     1081 ggcgcatgct ttgctgtcag tatcaaaaca aaagacaaaa aattgaaatc tacggctgtt
     1141 tctgcgggta tatctgcatt attcggaatt actgaaccag ctctttacgg ggtaacgatt
     1201 ctgcacaaac gtgttttata tgccgttatg ttttctagcc tgatcggggg agcatttgca
     1261 ggaatcgttg cacttcaagc ctttgcttta gtgggtcctg ggattgcaag tatcacgatg
     1321 ttcgctgaca aagctgaccc aatgaatctg gtttgggcat ttgcagtatc ggggatttca
     1381 tttgtggttg cttttgctgc agcgttggtt ctttataaag ataaagaagt tgtcaatgaa
     1441 gttgaaacta agatggaaac ggccgttgtt tcagatgctg ttctttcaag tcccgtgaat
     1501 ggaaaagtaa tctcactatc agaagtaaaa gacactgttt tttctagtgg tatggtaggt
     1561 gatggagtag caatcattcc tgaagaaggg atattagtag ctcctgaaga tggagaaatc
     1621 acaatggtat ttgaaacgaa gcatgctatt ggtttaaaaa cggctaatgg tgcagaactt
     1681 ctttttcata ttggtattga tactgttcag ttagaaggaa aagggtttga ggcatttgtt
     1741 cgttctggtg aaaaagtagc caaaggacaa aagctgattg catttgatat tgatttaatc
     1801 aaagaagcag gattcgaccc cacagttatc tgtgtggtaa ccaataagga taattacact
     1861 atccgttcat ttaacctaga aaaattggta accaatgata gtgatctctt agctatcagt
     1921 ccgatagctt aaacacaaga tagtgaaaaa aaatatattg aatgaaggaa tagaggagaa
     1981 tagaataatg acaaacacaa caagttttcc aaaaggattt ttatggggcg gcgcgatagc
     2041 agcaaatcaa gtagaaggtg cttggaatgt agacggtaaa ggtctttcag tagcggatat
     2101 ggctacttat aaaccaaatt tagacaacaa agattttgct gctcatatgc atgtcaccct
     2161 gcagtctata aaagaagcta gtgaagacaa atctgataaa tggtacccaa aaagaagggg
     2221 cgttgatttt tatcatcact ataaagaaga tttagcttta tttgccgaaa tgggctttaa
     2281 aaccttgcgc ctctctattg cttggactcg cttgttccct acaggagaag agctggaacc
     2341 gaatgaaaaa ggggttgcat tttatgaaaa cgtctttaaa gaaatgcaac gtttaggaat
     2401 tgagccgatt gtgacactat ctcattatga aatgccatta gctttagcag ttaaatataa
     2461 tggctgggtg gagcgtcgag taattgatga ttttgttcgc ttcgctaacg tttgttttga
     2521 tcgcttcggc aaatacgtaa aatattggct gacatttaat gagatagaca gcatccaccg
     2581 tcatccattt acaacagctg gaattatcca agagaaaagc gcagaaggtc aagaagctca
     2641 agatatctat caagcattgc atcatcaatt tgttgcgagt gcgttagtga caaaagatgc
     2701 tcatgaaaaa attccaggaa gccaagttgg ttgtatgtta acgaaattga tgacttatcc
     2761 attaacttgc gcaccagaag acgttgagct gaccttgaaa aagaacttag aaaataattt
     2821 ttatgcagat gttcaagtta agggagaata tcctgctcta atcgaaaagg atttggaaaa
     2881 tcggggaatt cagattaaaa tggaagatga agatcgagaa attttaaaga aacataccgt
     2941 agattttgtt tctttcagct attacatgtc catggcagaa tcagctgatc caaacgcaga
     3001 acgcacaccg ggaaatactg ttttaggggt taagaatcct catttgcctt caacagattg
     3061 gggctggcaa atcgatccaa aaggtttgaa aatttcgttg atcgaacttt atgatcgtta
     3121 ccaagtaccg ttgatgattg tggaaaatgg tatgggagca gtcgatcagg tagtagatgg
     3181 taaaatccat gatccgtacc gcatcgacta ctttaaagca cacttccaac aaatgaaaga
     3241 agcaatcgat gaaggtgtag atttgatagc ttatactagc tggggaccaa ttgatttagt
     3301 tagtgcagga acttctcaaa tgtctaaacg atatggattt atttatgtgg atgcagacga
     3361 tgaaggaaat gggacatata accgcttgaa aaaggattcg ttcaactggt acaaagaagt
     3421 gatcgaaaca aacggtgcat ccttattcga gtagtctcga tctttttaag cagcgatagg
     3481 gggataatga gtgatttcaa taaaaaaagt attgaattca agtgtggtac ttgttgaaga
     3541 aaatggcaaa gaaatgattg cattaggtaa agggataggc tttggacaga aagctgggga
     3601 tgaaatatca ggagataaag tagataaaat atttctgcct tttgaagaaa agaaatcttc
     3661 acaactatta gagttagttg aagaaatacc tattgaattt tttgaaatca ctcgtactat
     3721 cgtttttgaa gcagaaaaag gattagataa aaaactgaat cctggagtct atttaacctt
     3781 aacagaccac ctgcattttg cagttgaacg cgcacaaaaa ggggtaaata ttgcaaaccg
     3841 tttgtactgg gaaataaaaa tctactatcc caaagaattt caaattggga agtacgcact
     3901 cacccttttg aacgagcaat atggaattga tcttcctaaa gaggaagcct caaacattgc
     3961 tttccatttg atcaatgcac aatcggaaga tcctgatagc ggcgctggat tcaagtatgc
     4021 aaaaatgatc ggcgggatcg tcaatttggt ccggtattcg ttgcagaaag atatcgatac
     4081 taattcgatc cattatgccc gcttcattac acatgtacgt ttctttgtag aaagatatta
     4141 ttctgatgct ttgttggatg atggtgagga agaattctac aatcaaatgt ggatcttata
     4201 cccgtctgcc atggaaagcg cagctaagat caaagactac attgaaaaag tttatcataa
     4261 aacaattccg aacgatgaag ttgtatacct aggtgtccac atcaatcggt tgatgaaaca
     4321 tgctaatggg tgagcgctca gtaatactat tgatgctttt aaaatcttta gagatggatt
     4381 atagtaaaaa caaggtgatt tccagctgtt tgttatctat aagagacatt taaaaaggat
     4441 taacgtaaga taataaaaaa t
//
LOCUS       DAZUKT010000151         4458 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000151 DAZUKT010000000
VERSION     DAZUKT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.159"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG816_002939"
     CDS             172..516
                     /locus_tag="ACG816_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4921746.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG816_002940"
     CDS             517..936
                     /locus_tag="ACG816_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4921747.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="ACG816_002941"
     CDS             complement(1028..1378)
                     /locus_tag="ACG816_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921748.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2674)
                     /locus_tag="ACG816_002942"
                     /pseudo
     CDS             complement(1344..2674)
                     /locus_tag="ACG816_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            2930..3496
                     /locus_tag="ACG816_002943"
     CDS             2930..3496
                     /locus_tag="ACG816_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4921749.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="ACG816_002944"
     tRNA            3641..3728
                     /locus_tag="ACG816_002944"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            complement(3780..>4458)
                     /locus_tag="ACG816_002945"
     CDS             complement(3780..>4458)
                     /locus_tag="ACG816_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4921750.1"
                     /translation="LDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGIRK
                     GEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLLRK
                     YYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHTHA
                     SLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGESKG
                     ESKNILG"
BASE COUNT         1591 a          755 c          839 g         1273 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaatttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat agtagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatatcaacg attagacacg atttcgatcg tgtctttttt gttgtatatt
     3781 tagcctaaaa tgttttttga ctcccctttt gactccccct gagtactgtt cttcataaat
     3841 gctacaaaat tattgatgac ttttttgttt tgagtttcag ttacatgagt gtaaatattt
     3901 gaagtagttt gaatgtctgc atgccctaaa cgttcctgaa catcttttaa agaagctcct
     3961 gattcaaaaa ggagagaggc atgagtatgt cgaaaaccat gagtagtgat tcttttagtc
     4021 aattctggat gatgcttata tatgacatta agccacgaac gtgaaactgt agggttatag
     4081 agaagaccat catggtgaaa aattaagttt tccctttgga ttgtaacgat cgttttgttt
     4141 tttgtataat actttctcaa taaactgatc aaatcattgt ccagataaat ttttcttttt
     4201 ccagattttg atttaggagt atttacgatc aatcttcctt gataacctct tgttacagtt
     4261 ttattgatat tgagtgtttt gctcttaaag tcaatatccg accaggtcag agcaaaagct
     4321 tctccttttc ttatacctgt aaaagccaat agggaaaaga ataaatatct ttcatcatca
     4381 tcgtttctta ttgcttctaa aaatttgatt aattcttctt tagtataaaa ttcgatattt
     4441 ttatcttcga tatctaga
//
LOCUS       DAZUKT010000152         4236 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000152 DAZUKT010000000
VERSION     DAZUKT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.160"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..1868)
                     /locus_tag="ACG816_002946"
     CDS             complement(102..1868)
                     /locus_tag="ACG816_002946"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921751.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1828..3582)
                     /locus_tag="ACG816_002947"
     CDS             complement(1828..3582)
                     /locus_tag="ACG816_002947"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921752.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3692..>4236)
                     /locus_tag="ACG816_002948"
     CDS             complement(3692..>4236)
                     /locus_tag="ACG816_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4921753.1"
                     /translation="GLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYI
                     SYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQV
                     ASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVV
                     ASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1431 a          802 c          593 g         1410 t
ORIGIN      
        1 tcgtgtattc aaaattagat gctgtttaat aaaattattc tttttgaaaa agaattataa
       61 ctatatccca aaaattgtag gttaatttaa agcctatttc atcatcgtga ttgagcttct
      121 ttttcataga cgaatttctc agatagttta gttttcttta tgagattttt gtatatttct
      181 gaccttcgat acaactcact atgagtacct tgatcaacta tttttccttg atccatcact
      241 acaattttat ttaccttttc aatagacttc aagcgatgcg atataattag tactgtctta
      301 tcttgtatta aacgatttaa actttcctga atacgttttt cattttccgc atctaaactg
      361 gcagtaattt catctaaaat taaaattggc gcatttttca aaaaagcccg tgctatggat
      421 aggcgttgtc gctctcctcc agaaagtgaa cttccgtttt ctccgaccaa tgtatgataa
      481 ccgtcaggta gtttttcaat aaagtcttcg cagttagcta aacgagctgc ttttttcact
      541 tcctcatctg acgcttgact atttccaata cgtatatttt ccaaaatact tgtattaaac
      601 aaagttacct cttgaaaaac aatagcgatt ttgctaaaca aagaagctgg agatacacgt
      661 ttaatatcat acccatcaat taaaatacag ccttcatcat aatcatacag tctagacact
      721 aatttcaaga tactagtttt cccggaacca ctagcaccta ctaacgcagt cacttctcct
      781 tgtttagccg taaaagagat atgatctaaa attggggtat tgttatcata tgagaaagag
      841 acatctcgca actcaatgtc aaaagatttc agaggactat ctgatccctc ttggatactg
      901 gtttccttca tagctctaat tcgctgtatt ttaggagcta aataaaatat ttcaagcact
      961 gcttctttca tagaatcgaa ggaatccttt atttttattg cggctagtaa gtaaccaacg
     1021 acgtagagta tagtcacttc acccgttaac agtaagtgaa cacctactag aatgactact
     1081 gctaaactaa catatgagaa aatagaggat aaggccatga ctgaaaaagt acttaattct
     1141 acctttaaat gaatcctttc actctcttcc atttttttga aaagtctgtc ttgaactttt
     1201 ttagatagat taaagctatt aatctcttgc tgcaattcta tagtttcttg aaattcttcc
     1261 gagttttctc tcaaagtatc gtaatattta gtattggctt tcgtttggga tttctttgat
     1321 aacaagatta agacaaaact aaataacgtt ggcaaaataa cagctaatcc cattttgaca
     1381 ttaccaacca aaagcatcac tgaaataaaa gggaaaaaca gagccatacc gccggcttta
     1441 ggtattgcat gactcatcgc atgctcaata ccttcaacgt cagacatgat agtttgagat
     1501 aaatctgata aattatgttt tgaaaaatag gataatggta aatttgacaa atcgtctgca
     1561 atttgcattc ttaaatgcgc actttcttta taagtactat tatataagtt ttcatactcc
     1621 ctatctaaca accaatacat gaaaagcaaa gtaacgaacg atactgtaaa ataaagccaa
     1681 ggcggcttta cattttctaa aactaattcg tctatcagca tcattataat gaatgctggt
     1741 accatattaa tgcagtatac aaaaaagcta gaaatagatg ctttaaccaa atcttctgcg
     1801 ccctgattag ttaaagcgaa tttgttttta taaaaacttt tcattgtcaa gcctccaatc
     1861 atttgcattc tcataattag tgaccaattg tttatacact tgatgtttag ccattaattc
     1921 attatgattt ccacgttcta ctattttccc ttctgacaag acaagaattt catctacaga
     1981 tttaatgctg gataatcgat gagctatcat aataactgtt ttttctttca tcagatgttg
     2041 aaaagccttt tgtagttcat actcattatc tggatcaata gatgcactgg cttcatccat
     2101 aataacaatc tttgcatctt ttaaaattgc tctggcaata gctatacgtt gtttttctcc
     2161 tcctgacaga taaactcctt tactaccaat taaggtatgc tctctttcgg gaaatttttc
     2221 taaaatcgat tcgcaaccgg ctaatcttag cgcgtcaaat actttacttg ctggcgcttg
     2281 aggatttgct atagaaacgt tatcgtaaat actctgattg aatagtttcg tatcttgaaa
     2341 gacaaaagca attgtgttaa taattgtgtc ttttgaatag tgatttaaag gcttaccacc
     2401 aattttaatc tgtcccttat ccacattgta gaaacctgaa attagtttag ctatcgtact
     2461 ctttccgctt cctgattttc ctactaatgc ataaattctt ttttgttcta aaactaagtt
     2521 gaaattttct aaaacacact cttctttgta tgaaaagctg acattttcaa atgcaatgtc
     2581 acaattttta atctgtgtct cgttcccata ttttagttgg tcttgcttca tttctaaata
     2641 aagggagtct agtgtttcga ctgcatatct tgcttcaaac aagtgcatac ttacatacat
     2701 tattctcatg aaagaaacaa aaataattcc acttaaaaaa aacaacatca acaagtcaac
     2761 agctaatatc ttgggagtgg taagagaagt tataaaaaag gttataggaa ctatcaaaat
     2821 gcaaactatg ccaaaaaata gccattgaaa aagaacaaaa ggtgttttcc cactcaaaga
     2881 atacttatat gcaaacttag cataatcgtt aattgcttta ttcaaggctt taaatgatgt
     2941 tactttagca ccaaatatct ttataacttg aattcctcgg atatattcta ccgtttcaga
     3001 gcttaacaca tctaacgatt cttgatattt ttgaataaaa tttgtattct ctgtcatttt
     3061 ctttaacaag aatacaccaa ttactaacat ggcgattaga accaatccaa ttttaaaatt
     3121 aatgaaaaag cctaaggcaa ctgataaaac aggtgttact atggcttgag cactatctgg
     3181 aatcaagtga gcaattgcca tatgtgtttt agccgcgttg tcatcaattg tctttcgaat
     3241 atatcccgaa gaatttaaat caaaaaaacg aaaacttgct tctgttaaac cgctaattcc
     3301 cttttttctc aaattagtct ctaagcgaaa cccaaggata tgagaaaata agccagaaaa
     3361 aatataaaat aaagctccta aagttaacca agcaacagtt tgaaccgcat aactcttagc
     3421 taaatattca tctcctacaa taattagaga cttaagaaat ttgtaaatac tgtaatatcc
     3481 atagactgtt aatacgacag aaaaatcaga aaataaagtg gctaaccatc cgtagtattt
     3541 cattttaggg acataactaa ataatttttt gtatactttc attttttcct cccccgcatt
     3601 caatttcttc tcttattcgt ttacaactgt taatgctttc cgtaatagcg taaattagag
     3661 atgtacaaac agatagaagg taaactacgt tctatttttt gtattttttt aagacttttc
     3721 ttccaatcaa tacaccaatc atagataaac ttgctacaac taacactgaa atgattcccc
     3781 atatacctac cgcatagtgg atcattgcat ccattgttgc agcagattgt cctttatcta
     3841 tccaatcttg acgaaaacga tctgtaaaga accatatagg aatccactgt ccggcagcac
     3901 tgcctaattg cattagagcc caagacgcaa cttgaggaat tgctcgatca tatccaaata
     3961 cagatagaat aatatcagct aagatcgcca tactaatcga actgataaac caaggtaagt
     4021 acgccccgcc cattaacgta aaaaataaca ataaaacaat tgtgtaaata gtaaaaattc
     4081 cttttctagg gtagttatag ctaatataaa caaatatgat tccagataat aatccaaaaa
     4141 tccctgggga tatgctgtga ccaaacgccc caccaatact actaaccatt attgaaaaaa
     4201 agtaaattac tagggaaata actgtagcta aaccaa
//
LOCUS       DAZUKT010000153         4199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000153 DAZUKT010000000
VERSION     DAZUKT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.161"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(308..1813)
                     /locus_tag="ACG816_002949"
     CDS             complement(308..1813)
                     /locus_tag="ACG816_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4921754.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1810..2496)
                     /locus_tag="ACG816_002950"
     CDS             complement(1810..2496)
                     /locus_tag="ACG816_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4921755.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2689..4110)
                     /gene="gndA"
                     /locus_tag="ACG816_002951"
     CDS             complement(2689..4110)
                     /gene="gndA"
                     /locus_tag="ACG816_002951"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4921756.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1171 a          875 c          703 g         1450 t
ORIGIN      
        1 acactatttt ttataatttt tagtgacacc ttaaaggaat catttagaac aaataataat
       61 atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta ttatttttaa
      121 acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact tacatttaat
      181 taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat ccctgaattt
      241 ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa caagtaaagc
      301 acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa attgtccctt
      361 gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt tgtttagcga
      421 tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc actcgataaa
      481 aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag tcctgaatcg
      541 cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt cgtgtagttg
      601 aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga ttacgataaa
      661 tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag tctggatata
      721 atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt gtttcattcg
      781 cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga actaagcttt
      841 tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc tcaggatcat
      901 ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca ggtgtcctta
      961 actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa cgcatacggt
     1021 caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg tcgatttcag
     1081 gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct ctgagtacct
     1141 tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt aagatcaaag
     1201 aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca tagaaagaag
     1261 ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta gaatatatag
     1321 gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga actttacgtg
     1381 tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc tcgtccaaat
     1441 cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa tcgatcttca
     1501 ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca tttgtcgctt
     1561 tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta gccaaacgag
     1621 aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca atcagattga
     1681 tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg aagaaagaac
     1741 tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt tccgattttt
     1801 tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga tatagctttc
     1861 ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca ctacgtttgt
     1921 ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg ctaataccac
     1981 gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta gttcaattac
     2041 ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc gataagtaat
     2101 agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca acaatgcacg
     2161 aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat ctgctccgtg
     2221 gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa tgattggtgt
     2281 atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg ggagcatcag
     2341 atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag ttcggccgtt
     2401 gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa atcgtgccaa
     2461 gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc tttctattct
     2521 actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc tatagtcatt
     2581 ttatcatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat cgacttcagc
     2641 tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt attcttcatg
     2701 gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat gtgcgccaaa
     2761 gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt atgaatcgaa
     2821 gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag ccaaggcaac
     2881 gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat catctagcaa
     2941 taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca agaatcgagc
     3001 tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca agtcccaatc
     3061 aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca tgattttact
     3121 gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg tgaactcaaa
     3181 atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat aagcagaaat
     3241 atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt caagcgcgct
     3301 ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca catctactac
     3361 tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga tttctatcaa
     3421 atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt cttctactga
     3481 aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca tatctccata
     3541 ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc caatatatgt
     3601 gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg gtgcaactag
     3661 cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag ctccttcttc
     3721 accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact cttcattacg
     3781 acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc ctttatcaag
     3841 gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga ccattagcaa
     3901 gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg ttgctttgaa
     3961 atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac gattgaataa
     4021 agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca taacggccat
     4081 accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg ggatttctcc
     4141 ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac aacatattt
//
LOCUS       DAZUKT010000154         4187 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000154 DAZUKT010000000
VERSION     DAZUKT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.162"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..717
                     /locus_tag="ACG816_002952"
     CDS             31..717
                     /locus_tag="ACG816_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4921757.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            719..1663
                     /locus_tag="ACG816_002953"
     CDS             719..1663
                     /locus_tag="ACG816_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4921758.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1862..2506
                     /gene="pcp"
                     /locus_tag="ACG816_002954"
     CDS             1862..2506
                     /gene="pcp"
                     /locus_tag="ACG816_002954"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4921759.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2585..3862
                     /locus_tag="ACG816_002955"
     CDS             2585..3862
                     /locus_tag="ACG816_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921760.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1345 a          729 c          923 g         1190 t
ORIGIN      
        1 aaaatattgt cattttatgg aggaaaatat atggaaaatt atcttagcct tttaggcgtc
       61 ttgattgtta ttctcgggtt tgcatttaaa ttagactcga tattgatcgt aatggttgct
      121 ttagttgtta cagctttgac tggcggtctt ggcattcaag gaatgttaga acttttaggt
      181 acaagttttg taaacaatcg cgggatggcg atttttatca tcattatgtt ggcaacagga
      241 acgcttgaaa gaaatggtct gaaagaatca gcggcttcct tgatcaagcg attcaagaaa
      301 gtatctgctg gtatgattgt agatatttat gggatctttc gaatgatctt tgcggctttt
      361 aacgtgagtt ttggtggagt tgctggattt gttcgtccaa tcattttacc gatggcattg
      421 gggaccatcg aatcaaaagg gttagaagtc catcctaagc atgaagaaga actaaaaggt
      481 atggcttcag ctatggaaaa tgtctgctgg ttctttggcc aagtactatt catcggagga
      541 gcaggggcgt tgctagtcca atcaacactg aaagatctag gatatgacgt aacattggga
      601 cagcttgctt tagtggaagt tccagtggct atcacggctt tagttgtggc aagtgtctac
      661 ttctatctta aagaaaagaa attagcgaag aaatattatc caggaaaggg gcaataagat
      721 ggactttttt acaaatcctg atattttatt aggcgataaa ttactggaga tcctttatat
      781 cgtgatggga ctcgtattga tttacactgg agtaagaaat ttaacagata aaaccaatcc
      841 tcatcgttat gggtcagcct ttttctggac ggtcttaggg gtagttattg caggaggaag
      901 atggcttccg gcaatcgtga acggtggctt gatctttgcg atgaccatac cagcaattgc
      961 taagaaagtc agcaaaggag aaagccggct tccatcaaaa gaatatatgg aaaaaatgtc
     1021 agacaaacta ggaatgaaaa tttttattcc agctttgagt atcggtgtct ttgcgatctt
     1081 gtttgctcta tttaccaatt taggtgcttt agtcggcgtg ggcgtcggtg tttttgcagc
     1141 catgctaatc ttgatgttct tttctcgaga taatcgacca agtacatttt tagatgatgc
     1201 agcagatatg ctgggcacag tagggccgtt aagcatgttg ccaatgcttt tagcttctct
     1261 aggtgcggta tttacaagcg ctggagttgg gacagtgatt tccaatgcag taggtacgat
     1321 cgttccagaa ggaaatgtga acatcggcat catcatttac gctcttggca tggtgatatt
     1381 tacggtaatc atggggaatg catttgcggc catcaccgtg atgacggttg ggatcggtgc
     1441 accattcgta tttagtcatg gagcgaatcc agcgttaata ggaatggttg cattgacttg
     1501 cggtttttgt gggaccctgc tgacaccgat ggctgctaac ttcaatatcg tgcctgtagc
     1561 gatgctggag atgaaagaca aatacggagt gatcaaaaac cagttgttta ttgcgttatt
     1621 tatgctagtt ttccaaattg cttatatgat tctatttaaa taatagaaga gaggggttgt
     1681 gacagaataa ttgtcgcaac ctcttctaac attgtagtgg aacactattt ttttataaga
     1741 atatattaag tttgatctgt tatgttattt aaactattgt ttcctttatt tacgctgttc
     1801 aaggaaaatg gtatgataaa agataaccat atgttaaatc aatcggacgg aggaacaaca
     1861 gatgaaagta ctagtaacag gttttgatcc atttggtggg gacaaagtga atccagccta
     1921 tgaagcagta aaaaaaatgc cagatgaaat cagcggtgct gaaatcatca aagtggaagt
     1981 accgaccgta ttcgaaaaaa gcagtcaagt agtcaaagaa gccatccaac agcaccaacc
     2041 agatgtcgtg atctgtgtcg gacaagcagg tgggcggtca gcagtctcgt tcgaacgtgt
     2101 cgcaatcaat ctagcagaag caagaatccc tgataatgaa ggaaatcagc cattcgatac
     2161 ggctttagaa gaaaacggtc cagccgctta ctttacctca ttgccaatca aagcaatgac
     2221 aaaaaatgta caagatcatg gtctaccagc gtacatctct tatacagcag gaacatttgt
     2281 ctgcaatgat atcatgtatc gtttgcttca tttgatcgaa acacaattcc cggcaattcg
     2341 aggtgggttc atccacgtgc cattctcacc cgatcaagtg atcgatcgtc cagtgggtac
     2401 gccttctatg tctttagaag acattgcttc ttctttgact tatgcagttg aagcagcagt
     2461 agaaaataaa gaagatattt ccggaaatgc cggaacaact cactaattta gcaagaaaca
     2521 ctggctggtt gctaacgtta tgttagtagc cagctttttg taattagaaa ggaaagaaac
     2581 agccgtggaa gaatggcaat ccgtctttga agaatggttt ccaaaagaaa tcagcaaaag
     2641 ctatcccatc aaaatttcta aacaatacac aagcagtcaa cgttgggaga tctatgcaaa
     2701 attaacgaaa aaacagcggg aactagtcga taagcaccgc cgatatctaa tcagctctcg
     2761 ttttatggaa gaacattatt tagctgcaac agattgggtg ttttctgact tcaagatcaa
     2821 tccgtttttt cgaacaaaac gaagccagca aaaattgtat tgtgagtgtg gtagagaatt
     2881 aaaagtgcaa tacatcgtta aatcgccaaa aaccggaaag atcctaaaac taggcatcaa
     2941 ccactttgcc gatcacctgc atgtctctcc gacagtggca gcttcgatcc atcagggaat
     3001 gacaaaagtt gatctggcct tagacgaatt attatggctt aagcagaaga atatcgattt
     3061 tcctgaagga ctatggcaaa aatactgttt tgtcttgtac caaaatcgtc ggatgaaaca
     3121 gccatattta ccggatataa aattagctca gcggctcgca gaatttcgcc aagtagaaat
     3181 gccgatttac atagcggatt atcaagcatt ggaaaatgaa atcaagaaaa taagtgaaca
     3241 catcaatggg cagtcaaaaa agcgacagat aaaaaaagaa ctatttgacg actttgcaga
     3301 agaattagta aaggatgtag aagaattctt gatcaactat cgcgcatttt tacgtaaaga
     3361 ttggcaatcc atcgtatatg aagaagtgcc agtacatcct aatgcttatt ttgaaacatt
     3421 tatttccgtg ttgagaaaga caaaacgtca acgaacacca gaagtcactg ctcagatgga
     3481 atattttgcg aaaaaccaac gattcatcca accaaaaatc tatctattta tttggaagca
     3541 atattgtcgc tatggtttta cagaagggtt cttcgacagc attcctcgga tagtcagaaa
     3601 tggtttttta aaagtattgc gtaaagaacg agaagctata caatctgctg ataagaaaga
     3661 ccgtacagtc tcaaaagaaa aatggcaact agtagtgaaa gatatccaat ctggaaatgt
     3721 gcaagagaca atcgacaaat ggaaaggaaa acattatcgt tttactgaag ctcagaaaca
     3781 agcgttggaa tattatcaga agttagaaga atcactgcgt ttcaatgatg aggcaagaaa
     3841 gtatctgaag gaattactat aaaaaacata ctaagattag aggggaacat cattgacgaa
     3901 tgatgttctc ctctattttc tatttttaga ttatagtgaa gagtgaaaga aagttcgaac
     3961 aaagccttga ggactacgag tccctatcaa aaaccgaact tctttactgt cattttagaa
     4021 tcaggtttac gtatgttggg gccatgagcc cgtgtatagg aaattggaat cgaaatgaag
     4081 cagtaaagtt ctttcaaaac ttaaaaagga ggaaaacaaa tgccttacat tcttgcatta
     4141 gatgtttcaa tgggacacag ctacagtgtc ctatacaaag acgacac
//
LOCUS       DAZUKT010000155         4151 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000155 DAZUKT010000000
VERSION     DAZUKT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.163"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACG816_002956"
     CDS             <1..106
                     /locus_tag="ACG816_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4921761.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="ACG816_002957"
     CDS             complement(359..508)
                     /locus_tag="ACG816_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921762.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="ACG816_002958"
     CDS             970..1182
                     /locus_tag="ACG816_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921763.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="ACG816_002959"
     CDS             complement(1460..3259)
                     /locus_tag="ACG816_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4921764.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="ACG816_002960"
     CDS             complement(3383..3979)
                     /locus_tag="ACG816_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4921765.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1226 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt t
//
LOCUS       DAZUKT010000156         4116 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000156 DAZUKT010000000
VERSION     DAZUKT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.164"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..316
                     /locus_tag="ACG816_002961"
     CDS             155..316
                     /locus_tag="ACG816_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4921766.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="ACG816_002962"
     CDS             335..1612
                     /locus_tag="ACG816_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921767.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="ACG816_002963"
     CDS             1622..2278
                     /locus_tag="ACG816_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4921768.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="ACG816_002964"
     CDS             2278..3654
                     /locus_tag="ACG816_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4921769.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..>4116
                     /locus_tag="ACG816_002965"
     CDS             3784..>4116
                     /locus_tag="ACG816_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4921770.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1629 a          455 c          727 g         1305 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAZUKT010000157         4070 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000157 DAZUKT010000000
VERSION     DAZUKT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.165"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1435
                     /locus_tag="ACG816_002966"
     CDS             86..1435
                     /locus_tag="ACG816_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4921771.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="ACG816_002967"
     CDS             complement(1563..2153)
                     /locus_tag="ACG816_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4921772.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="ACG816_002968"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="ACG816_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4921773.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DAZUKT010000158         3924 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000158 DAZUKT010000000
VERSION     DAZUKT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.166"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..1417
                     /locus_tag="ACG816_002969"
     CDS             182..1417
                     /locus_tag="ACG816_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921774.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1501..2589
                     /locus_tag="ACG816_002970"
     CDS             1501..2589
                     /locus_tag="ACG816_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFS4921775.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            2586..>3924
                     /locus_tag="ACG816_002971"
     CDS             2586..>3924
                     /locus_tag="ACG816_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS4921776.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILII"
BASE COUNT         1383 a          427 c          679 g         1435 t
ORIGIN      
        1 aaatttactt ttatttgatg actggaaata gaaattaaat atattgtatt gttacattaa
       61 agaaaacttg tatggaaaaa tcagtacaat accattattt aatgttcttc acttaaagcg
      121 caaaaaatat cagtttatta aactcgtata tagaacaaat atatgtagac ggggcgaaac
      181 gatgagtgta aaaaaaatgc tatttatggt aagtacaata attgtattaa catcccgatt
      241 ttggggaatt aatactagtt tatctatgcg atattttatt atggcaatat gggatttata
      301 ttttatgtta agctattttt cttatcgtga gaaaacatat aaaaaatacg ttatcaagaa
      361 taacttttgg ttgactataa tgccgtttgt gatattttct atctatactc taataatatg
      421 gggaattggt tcaaatgcag aatttgaaaa ttttataaag ttatgttcca cgttgcttta
      481 tttaattctt gcatatggtt acgcatgtac agcattttac ttatttaagg cagaaggaat
      541 tgattttatt ttttggtcag gtgttatttc atatatgagt ggttcagttt tttatttaat
      601 tgtatcttat ggtttaaatg gtttaatttc ttatataaaa agtcttataa caggtgaaac
      661 aaatactgca aattttgcta tggaggtaca tgatttaact tttgcgatgg gggttttttt
      721 cctttattac gtattttttg aaaataaaaa tacaaaaaaa cataagataa aaattatcgt
      781 atctttaatt ttaattttca tgggattgaa gagaattgaa ctgttagctt taagtggtgc
      841 aattctagtg tattatgttt tattaaagtg gggaaaaaag attcaaatcc gtgcattagt
      901 gtgtactatt tgtgcaactg ttatttcgtt agtgtttatt tacattatag atacaggaat
      961 tttggcattg cttatgtcaa atttgggcat tacagatgac ggtgcaaggc tttcatttta
     1021 tctatattca tctaaatttt atgaacttgg tttaggatat gtaggaacag gatggacatg
     1081 gttttcaaaa tactttttta atctttattt atcgcacttt aggttaaatg gtggaagaat
     1141 tgcagcaagc ctacacagtg atatattaac tatttatata gaagttggat gtatagtttt
     1201 tattatatgg atgttttggt tattttttgt gaaaccgata agactaacta aaaaatttgg
     1261 attgaaaaca ggggagtgtg tattattatt aactatatat atgtttatat tatatttgac
     1321 ggataatacg ctcacttatc cagacactca aatgattttt ctcttagtac ctatgatagt
     1381 ttcgtgggtt gataatcaac aacgaatatc ggaatagata gctaaagtat attttaatta
     1441 tgaatttata attagttgta aaagttaata tttaacggac aggaagtagg gtgaaaaaat
     1501 ttgaaaaaaa ttttagtgtt agcaaataca tattatcaaa taatagtagc aatacaaatg
     1561 aaaaatacaa ttttttccga tgagtatgtt gaaattatta tttctgatca ttctaaaaac
     1621 actaaagaaa ttgcgaaaaa aatagaagaa aaaaaagtgt ttgatggctg tcattattta
     1681 gaaagcaaat cttttgatga taatattggg tattttcaaa agattaagga ctgtcttcag
     1741 atttcttgtg gtaaatcaaa tcgatatgcc tattatttga aaaactgtaa aagtgaatat
     1801 tttgatgaat ttatttgttt ttcaatggga gaggtttggt tggaaggaat atatgcttgc
     1861 ttatatcgaa ataataaaaa tgtacaggta tcaattttag aagaaggtat tttaagctat
     1921 ggagttgaca ttattccaac agcaagaaca aaagctatcg cagtactcaa ttacttaaaa
     1981 aaaaggactc cactatataa caaaagctat aatttttatt gtttttttcc taatatatat
     2041 acaggaagag agaatacaat acaaattcca ttagttacag ctgggtcaag aaccgcattg
     2101 caactttcgg atatttttaa ccctatgata gatatttata aagaaaaaaa atatatattt
     2161 tttacgtctg tttatgattt tgagggcgga gaacctgtcg gagaatatga attagtatgc
     2221 aaaatagcag acttagtagg aaaagataat ctaattgtta agacgcatcc tagagatgta
     2281 agaacaattt ataaagataa tggatttatc gaggataaaa attcatcaat accttgggag
     2341 gcgattcaat tgtcaggcga ttttaacgat aaaatattta tgacaattaa ttcaggaagt
     2401 gttatttcag gtagtacaat gtctgcaaaa cctgtacgca cgtattatat gtataaattg
     2461 tgtaacatag aaggaaatcc gtcttgcaaa aagaatgcgt atgatattga gcgattgctt
     2521 aagcaaaaaa cgatgaaaaa cattttaaag aatgtgaaaa tagcagaaag cttagatgat
     2581 atactatgag caaaactaaa tatttaatta aaaatatggg aatattaaca ataagtaatt
     2641 tttcttcaaa aattttagtt tttttgttag tccctttata tacaagtgta ttatccacaa
     2701 cagaatatgg cacatatgat ttgacaattt caacagccac attattgttt ccaattttaa
     2761 ccttaaacat tgtagatgct gtgatgcgct ttttaatgga taaagataaa aataaaaaaa
     2821 atatagcatc tatagcgatt aaagttatat ttttaagcgt gatagtcttt ggatccgtaa
     2881 tgtatttgtt aaatattaca aaattatggc aagatgttta tggattagaa aaatatattt
     2941 ttttgtatta tatatcctat gtatggaatc aatttctaat acaatttgct aaagggttag
     3001 aaaaagttaa agctatgggc atagcaggta ttatcagcac aattgtaaca ataattgcca
     3061 atattttctt cttgcttatt ctgaaatggg gattaattgg ttttttcatt gcaaatattt
     3121 tatcacagat tatttcagct acttatctgt cagtattagt gagaatatgg gaatatatcg
     3181 attttgaggc taaagatcct cagctaaaga gagaaatgct aaattactcg attccattaa
     3241 ttgcaactgt tgttggatgg tggattaata gtacagctga taggtatgtg gtttccttta
     3301 tagtaggtgt ctcggcgaat ggcttattgt ctgtggcata taaaatacct caaataatta
     3361 atactttgca gggaatattt atacaggcct ggcaaatttc tgcaattaaa gaatatggag
     3421 ataatgagac tgcaaaattt tatggaaata catttgttat gattaatttg ttaatgtgca
     3481 taacctgttc aagtcttatc atattaacta gaccgttttc acatattctt tatgcgaaag
     3541 acttttatga agcgtggaaa tatgttccat ttttattaat agctaatgtt ttaaattgtg
     3601 catcaggtct gttaggacct atcttatcag caaaaaaaga ttctaaatcc atgatgtggt
     3661 ctgcaatcat aggggcatta gttaatgtga ttatgagtgt agtccttgtt tataagatag
     3721 gggttcaagg tgccacaata gccactgtta tatgttcata tgtaatatat gttgttcgta
     3781 aacgagctgt gggaaaagat atttttataa agcattatca tattgttgtg gaatcttggg
     3841 gattattgtt tgttcaggca atcatagaaa tatattttaa aaattatgta atagaaatta
     3901 taattatgat tattttaatt atta
//
LOCUS       DAZUKT010000159         3922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000159 DAZUKT010000000
VERSION     DAZUKT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.167"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..301)
                     /locus_tag="ACG816_002972"
     CDS             complement(50..301)
                     /locus_tag="ACG816_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFS4921777.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(315..506)
                     /locus_tag="ACG816_002973"
     CDS             complement(315..506)
                     /locus_tag="ACG816_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921778.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            799..1119
                     /locus_tag="ACG816_002974"
     CDS             799..1119
                     /locus_tag="ACG816_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4921779.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1137..1559
                     /locus_tag="ACG816_002975"
     CDS             1137..1559
                     /locus_tag="ACG816_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4921780.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            1642..2277
                     /locus_tag="ACG816_002976"
     CDS             1642..2277
                     /locus_tag="ACG816_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4921781.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            2395..3606
                     /locus_tag="ACG816_002977"
     CDS             2395..3606
                     /locus_tag="ACG816_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4921782.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
BASE COUNT         1442 a          628 c          691 g         1161 t
ORIGIN      
        1 ctatttcccc aaagccctaa agcgtagatg attagcggtg cgctgaatac tagtgctctg
       61 ttgaatttgc ccatgttttt cctcctttta gatactgata caaagcgata ctactaaacc
      121 tccactctct gccaactttc gctgctggaa tctttccaga ttcggcatct ttagttagcg
      181 tgcgtgtcgt ggttttcaaa tattccgcag cttgctttgt atcccacact tcatttgcaa
      241 tctcagattc tgccaaagaa gctttgaggg ctgagaggtt aactaaagct agttgttcca
      301 ttgattaatt cctcctatcg aattctgtaa tcacggatga tttctaagat agttttatta
      361 gcttttggtc cactccaatg gccatcaata atttgctgca ttcgaacacg tgtgtatcca
      421 tatgcagtag ccaaatcttc catagttact ccgttttctc gcataaactt cttgattgca
      481 gcacggccgt tatctagatt tgacatctat tcactcccct tacatataga tttgtaagtc
      541 aaaatgatag aaaaaacgta taaactattg actaatagta tacaatcgta tactatataa
      601 acatagttaa ataagcctac aacaaaccct ttattatgca atcggtcgcc aaacttaatg
      661 ctataaggtg tatttttagt ttgctttttt tctatcaaat taacttacaa acaaataata
      721 atacaaactt atacttatgt caacagtata atttacattt tgtgtacttt tatttgttta
      781 ggattggaga acattattat gacactgttt gaaaggataa aatcattagc tagccaaaga
      841 gataaaagca tgaaagaagt cgctttagaa ttaggattta gcgaaaatct tttctatcga
      901 tggaaaacaa cagaacccaa agcaagagat ttgcaaaagg tagctgacta tttcaatgtc
      961 tctgtagact atcttctagg tagagaagaa aaagaaacgc ctaaacatgt ggatttatca
     1021 gaagatgata ctgtattttc ttttgatgga aaagaaatat ctaaggagac aatgcgtaaa
     1081 gcgattgcaa ttgctaaagc tttagaggaa aatgaatagt tggagtgatg ggttgtatgt
     1141 atttaaagtt gaaagaaatg ctgagtgagt ataatttaaa gttaatctat atggaaatgg
     1201 aagaaccagg tttttattat ccaaaaccaa gaatagtatt tttgaatgaa aaactacacg
     1261 aagacagttc tgaagctttt catttagccc atgagctcgg tcatttcatt gcttcacatt
     1321 ttgaatattc agtactgtac gataactcta caacttttca ttcaaaattc gaagctgaag
     1381 ctgatagaat cgcaattatg attttactaa atatctttat tgagaacgaa ttgactgatg
     1441 aatctcagtt caaattagaa aatttcatgg aattctactc tatcaataat aagttaagaa
     1501 cagaatgttt taatgtttgc cagtcatatt tcaagaaaaa atactcttat gcacagtaaa
     1561 aaaagcccgt gctgcaacac ggactcatac ctcatttctg agatcacaaa tatattataa
     1621 caagaagtga gggatattta aatggcaaaa aaagttatgg gtcaagatgg gaaaatgtat
     1681 aaggttaaaa aaccgtttta taaacgggta tggttttggg tattaatagt acttttgttt
     1741 tttggtattg gtggtgctct aagtggcggt gaaaacaaag attcagctac tgcaacatca
     1801 acaagtacta ataaagaaac tacaaaagaa actacaacaa aagaagaaaa gacttataaa
     1861 atcggtgatg acgttgctgt agggaaaatg gaatacaaag taaactccgt tgaagtagta
     1921 aaacaagttg gcccatctgt acttcctact aacgcaaaag acactttctt agtagttgac
     1981 ttatctgtaa aaaatgcagg tgataaggct gtgactgttg atagctcatt cttcaaatta
     2041 aaagcagatg gaaaaacttt cgaagctgat tcggctgcat ctatgtcggc aaatcaagat
     2101 gaaaatggta atatcactaa ctctttcttt atggaaagct taaatcctga tatgcaacaa
     2161 actggtaaaa tagtttttga tatctctgaa gcccaagcaa atgcacaaaa taatgtattg
     2221 caagcacaaa caggttactt tggaacagag acagtttcaa ttgctcttca taactaatta
     2281 gtataaaaaa cacgccccac cgaccaaagc gagcgtgttc taagaaaaaa caaacctaca
     2341 caataggctt ctttatagta cctattgtat cagagaaaga gagctgattc aattatgtca
     2401 gaagtaacag gatacttaga acaagtagac aatgaaacat atagactaag agctgtatta
     2461 gggtacaaac ctgatggctc ggcaaaaaga aaaagcaaaa ctataaaagc gaatagtaaa
     2521 agagcggcat ataaagaatt gaacatctgg ttagagcaat tcgaaggaat gaccgatgat
     2581 tcgttggatt tattcaatat aacttttggt gaattttata gaaaaatatg gctttcagag
     2641 gccgaaaaaa atttagagcc aaagtcttat cataactata agcgaatgat cgagaataga
     2701 tttcttgata aatttgattt tatccctcta atcgatataa aaccatacat gataaagaaa
     2761 attgttgtta atgctcaaag aatcaataca aaagatcctg gaagaaactc tgataaacct
     2821 ttatcaagaa atacaaagct tcgtatgtta tacgccgtta acaacttatt tttgatggct
     2881 aaaaatgagt acggagcaat taaagaaaat cctgtggaaa atgtaaaaat accaaaagaa
     2941 aaaggcgtaa aaaagaatat cgaagaacct tattcggaag aagaaattca tgcaatgcta
     3001 aaagcagcat ttgaggaaag catagaaatt aaaactttaa ttgttctagc atttattaca
     3061 ggagcaagac aaggagaaat tgcagcttta gaagaaaaag atattgactt tgacaaacaa
     3121 gaaataagat ttcatcagcg gatttcagaa gtggatggaa aatcagatat tcggttgctg
     3181 ccaggtttga aaaatgatga tgatgaaaaa atagtaaccg gtcctgctta tctatttgat
     3241 atgttagatg aattgatcaa agagaataaa aaaattcgtt ggaaattaaa tataaagaaa
     3301 ttaagacact attttatatt tgatacaaaa caagacggaa ctttaccacg tggaagttat
     3361 ctgtataaga agttcaaacg attcaccaaa cgtcacaatt tgcgtcacat tcgttttcac
     3421 gacattagac atacatcagc tacctatttg cttagcgatc ctaatatgac tccaaaagag
     3481 cttcaaaaac gattaggaca ccgagatttt aatactacaa tgaatgttta tggccatgtc
     3541 ttacgcaaag aaaaagatac agcaactaca gcgtttgaaa aactattaaa aaaagatata
     3601 aaataacact aaaaaattat attttcgggt ataaactcgg gtaggatata taaaaacttg
     3661 ggataatttg cggtatataa gcatttaaaa atgctgtaaa ttcgcttatc ttcatttaaa
     3721 ataccattaa ataacgaact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
     3781 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
     3841 cgctgttctt tttttatttt cgccgtttgt ataattaagc gagacaaata aaaaagccat
     3901 ttatgttaca ctcaaattgt aa
//
LOCUS       DAZUKT010000160         3821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000160 DAZUKT010000000
VERSION     DAZUKT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.168"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            362..961
                     /locus_tag="ACG816_002978"
     CDS             362..961
                     /locus_tag="ACG816_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4921783.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1010..1726
                     /locus_tag="ACG816_002979"
     CDS             1010..1726
                     /locus_tag="ACG816_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFS4921784.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1740..2519
                     /locus_tag="ACG816_002980"
     CDS             1740..2519
                     /locus_tag="ACG816_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS4921785.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            2602..2958
                     /locus_tag="ACG816_002981"
     CDS             2602..2958
                     /locus_tag="ACG816_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4921786.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            2948..3214
                     /locus_tag="ACG816_002982"
     CDS             2948..3214
                     /locus_tag="ACG816_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4921787.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            3373..3651
                     /locus_tag="ACG816_002983"
     CDS             3373..3651
                     /locus_tag="ACG816_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921788.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(3648..>3821)
                     /locus_tag="ACG816_002984"
     CDS             complement(3648..>3821)
                     /locus_tag="ACG816_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4921789.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         1412 a          547 c          741 g         1121 t
ORIGIN      
        1 tattggatcc ataacatgac taaattctga atacttttta aagcttatca tttggtaaaa
       61 ctgaatataa gttaattata tagactgaaa attttgaatt ttcagaatag aataacttaa
      121 aagtactgac aaatttttta aaggggcaat attaatgttc tgagataatc aaatcttacc
      181 gaaaatatca aaagtccaat gtgacacaat tggattttgt cacattcgtt aaaatcgctc
      241 catccttaga ggctctaata ggaaatcact tttcttttag cgcctttttt ctatagcttg
      301 tttaaatgtg acttattatc tgttataatc gtattaagta acatttgaga ggtgtctaaa
      361 aatgaaaagc gtggaaccga ttcgcgacaa aaaaaagatc gatgccatga aggccatttt
      421 ggcttccgga aaatatgggc aacgaaattt ggtgcttttt tcgatcggga tcaatacagc
      481 ctatcgaatc tccgatttaa gacagttaaa attaagggat gtgctagaaa tttcccgtgg
      541 gcgggtgatc gtcaaagaac gtctggccat gaaggaacaa aaaacggcca aacacaattc
      601 ggtcttcatt tcgaacaaat tgcgaaaagt gattcttgat tatgttcaat ccgaatttcc
      661 tgagcagttg caagcacaag acttcagcaa gtatttgttt ccaagtcgaa aaggagcaga
      721 tacgcctttg acccgacaaa gcctttggcg gatcattcat gaagcaggaa ctgcagttgg
      781 gttaaaagaa attggtcccc attcgatgcg taagaccttt ggctattttt tatacaagca
      841 aggaaccaaa acagagatca ttcagtcgct gctaaaccat tcttcacaac gggagacctt
      901 acgctatatt ggaatcaccc aggaagacaa agatactgca gtaaagagtt tggatttatg
      961 acagaataat gccctatata atagaaggaa aaattcaaga ggtgagaaaa tgaacgaaga
     1021 aaagcagcct aactttcctg ataaatatca cttatctcgc aaagagtctg tgtatttatt
     1081 gaagaaaaac attgtagaac tggtgtataa tgcggggaaa tttgaagggt taaatacaac
     1141 gttgttacaa acagaaggga ttattaagta taatcgggcc aacaacgtgg ctgtagatga
     1201 tgtcctgacc gtcgttaact taaaaagagg ctttgaaatg cttttaaatg acgtacagga
     1261 gcccctgtta gagacaagta agcggattaa tcggatcgtt gcagcagagg atgcgctttt
     1321 tcccggtgaa atacgaaccg gtggggtaga attatcgacg attcaaggaa gatatgtgcc
     1381 accaatgtta actgaagacg aggtaaacaa tcaatatggt gagataatga atcaagagat
     1441 atcagatacg gaaaaagccc tgcgactgtt tctattcatt tcgaaaaatc aaatattctg
     1501 gaatggtaat gtgggtacag ccttgttgat tgcgaataag attatgttta gcaacggttt
     1561 aggactatta tcaatacccg aaaatgtatt tatgaaattt aacgaacttt tatcaaagta
     1621 ttgcaacagt aatctattag tcaatgagtc aaatattata tcatttttat atgaacagtg
     1681 tatctttggt attaattatt tgaaaataat ccaacataca atttaagagg tataagtaaa
     1741 tgatgactat agacgaagca aaacaattag caacttattt ttcagttggt tcagcgggat
     1801 atattttaga ctttactaat gtaacgtgga acatatttac aaagaaaatc gtagggattg
     1861 aaatacaaaa tcattatggg ggctcaaagg gagtatcctt tcaaaagttt ctgcttgacg
     1921 atcaatttga tgattcgtta aagaatcggt taatccaatc tttaaaaggt attatggatc
     1981 gagataaaaa atatgaatct actgaaatga atcccatagt tcagttgctt tttgaggaag
     2041 tatttaaaga agatacttca actgcattaa gaaaaatgaa attttcaagc gagattgatt
     2101 tactttatat tcggcagcta cccattagga ttcaaacaga tattaaaaac aaggattttg
     2161 actctgtttt gacaaaagca aatactttaa tagatgaagt tctaaaattt attattgaaa
     2221 ataccgaagg accgagtgtt aatattaatt tatttaagtc aaaagaatta agaaaagaag
     2281 tatttggccg cctcaatatt aaaactgaaa aagatatgga taatagaatc aaagcccttg
     2341 ttggagcact caatacatta tcagataaaa tactcgaaat gagaaatagt caaggagatg
     2401 ctcatgcaca tggctcaaat agaatagtaa ttaaggaaga agaagcaatt cttgttgcaa
     2461 attcttctat ggttttgtgc gagtatgtgt ttactaagta taaaggatta aatagataat
     2521 aaaatgcata ttttatagta tacaattcga atttttatgt tatatactat aatataaaat
     2581 caatatatga tataattggg tatggaaaaa atatcttttg aaacatttaa aagacccaat
     2641 ggtcatgatg aatttttaga atggttggaa acattaccaa aaaaagatag tgcgaagctt
     2701 ttacgtacaa tagaagaaac agaaaagaat gggatgctag tagctcaacg gttgaaatgg
     2761 gtgaaaaaat tagacactaa tttgtacgaa ttaagaagta aggtaggatc aaacatccaa
     2821 agagccatct atttccatgt ggacaatgga aggtatgtca ttactcacgg atttactaaa
     2881 aaaacggaga aaacaccaat aactgaactc aagcatgcaa aagaacttag agaggagtgg
     2941 ttcacaaatg atgattaaaa aattgattga agaaaagaaa aaagataaag attttgcaga
     3001 agcatatgac cgtgaaggaa gaaaactcga aactgcggtt gctctttatc atgctcggga
     3061 ggaagcaggg ctaactcaag cggagcttgc acaaaaagca catacaactc aatctactat
     3121 tgctcgaatt gagagagggg ataacgtatc tttcgataaa cttgatgata tcgccagggc
     3181 attaggtaaa aagctggtag tagaatttgc ttaaaagcga gttgaaaata tatctataat
     3241 aggctcctaa ctaatcaatt agtttagtgg gccttttttt actagataga taaaggttgt
     3301 actatagtgt aaaagacaat aaattttata ctaaaattac tttaaagaga actagagaga
     3361 aaggtacaat aaatgaaaac cataaatctt ataaaagtat ttatagcatc accaggtgat
     3421 atagcagcaa aaaggaatga agtagaagaa accatatata gatggaatat agaaaataca
     3481 gataatacaa aagtgatttt gatgcctata cgatgggaaa ctaatgcgat tcctacttat
     3541 gtaaataata gctctgggca aagtgttatt aataaacaaa ttgtagaaaa tagggttctg
     3601 ctgcaaagtt ttaaaaataa aatcagtttt tgtattattt cttcatttta agctggaatt
     3661 cccaataata tcctgatttc agtgcagact gaaaagccga agagagtgcc ttcttttcgg
     3721 cttttcttgt agattcctcg aatggtttcc atccctttaa ttgtggtgga agctgtacgt
     3781 agactttgat acaatttatg tcgacgtttt atcgatcgat g
//
LOCUS       DAZUKT010000161         3751 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000161 DAZUKT010000000
VERSION     DAZUKT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.169"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..478
                     /locus_tag="ACG816_002985"
     CDS             <1..478
                     /locus_tag="ACG816_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4921790.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="ACG816_002986"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="ACG816_002986"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4921791.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="ACG816_002987"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="ACG816_002987"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4921792.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..3697
                     /locus_tag="ACG816_002988"
     CDS             3200..3697
                     /locus_tag="ACG816_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4921793.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1239 a          598 c          811 g         1103 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
     3661 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
     3721 aagagaaatc aagaataaaa agagccggaa a
//
LOCUS       DAZUKT010000162         3571 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000162 DAZUKT010000000
VERSION     DAZUKT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.170"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..970)
                     /locus_tag="ACG816_002989"
     CDS             complement(266..970)
                     /locus_tag="ACG816_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921794.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1157..1402)
                     /locus_tag="ACG816_002990"
     CDS             complement(1157..1402)
                     /locus_tag="ACG816_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921795.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1419..2270)
                     /locus_tag="ACG816_002991"
     CDS             complement(1419..2270)
                     /locus_tag="ACG816_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4921796.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2436..3440
                     /locus_tag="ACG816_002992"
     CDS             2436..3440
                     /locus_tag="ACG816_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4921797.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1111 a          609 c          692 g         1159 t
ORIGIN      
        1 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
       61 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
      121 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
      181 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
      241 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
      301 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
      361 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
      421 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
      481 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
      541 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
      601 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
      661 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
      721 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
      781 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
      841 gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata
      901 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
      961 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
     1021 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
     1081 gtattttccc gttttttgaa agaaaaataa aaaatggaac ggattatgta tatatacata
     1141 atccgttcca ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat
     1201 aaatgacttc atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca
     1261 gttcttcgat gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg
     1321 catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat
     1381 tcaagcgttc gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg
     1441 tcttttgctt tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt
     1501 aattcaaaat caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga
     1561 agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca
     1621 tgtgcttcag cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga
     1681 ctatatgctt cgaggatcat atcatgtttt tttgcatact caactacagt tgtctgtaat
     1741 tcgcctggtg caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt
     1801 gtatctagat ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca
     1861 tacagttctt caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa
     1921 gcaacagggt ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc
     1981 aatgattctt caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt
     2041 gttgtcaaga atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacgctt
     2101 tcttcattct tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct
     2161 ttaacagagg aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc
     2221 gggatttcgt aaccatttga tagacgataa gtgcttgtta atgaattcat actttcagct
     2281 ccttttttat tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg
     2341 tttctactct tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt
     2401 tttttccgta aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa
     2461 ttctgcatat tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc
     2521 tgacaaaaga atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc
     2581 aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc
     2641 aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag
     2701 aaagtgaaga agcacctagt gcagatgtat ttgttgtctt gtatgaatta gatacagtga
     2761 tgtacttatc tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag
     2821 acgaagcagg gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac
     2881 aaaaagcaga tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa
     2941 aaaaatatga gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg
     3001 ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg
     3061 gaaaaaaatt tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg
     3121 atacgataga agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg
     3181 aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag
     3241 aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat
     3301 tcaagctatc aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt
     3361 tgattgaatt tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg
     3421 aagtattgaa tcgtttataa aaatgactag aatattaggt gttcaagtac cagtgatcag
     3481 ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aaagagcgtg
     3541 agacaaaagt aaagaacact tttgtctcac g
//
LOCUS       DAZUKT010000163         3480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000163 DAZUKT010000000
VERSION     DAZUKT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.171"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="ACG816_002993"
     CDS             complement(<1..2303)
                     /locus_tag="ACG816_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4921798.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACG816_002994"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACG816_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          958 a          776 c          558 g         1188 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
//
LOCUS       DAZUKT010000164         3361 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000164 DAZUKT010000000
VERSION     DAZUKT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.172"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            677..763
                     /locus_tag="ACG816_002995"
                     /pseudo
     CDS             677..763
                     /locus_tag="ACG816_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            1317..1541
                     /locus_tag="ACG816_002996"
                     /pseudo
     CDS             1317..1541
                     /locus_tag="ACG816_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1801..2055
                     /locus_tag="ACG816_002997"
     CDS             1801..2055
                     /locus_tag="ACG816_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4921799.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            2056..2372
                     /locus_tag="ACG816_002998"
                     /pseudo
     CDS             2056..2372
                     /locus_tag="ACG816_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2931..>3361
                     /locus_tag="ACG816_002999"
     CDS             2931..>3361
                     /locus_tag="ACG816_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921800.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGN"
BASE COUNT         1211 a          462 c          565 g         1123 t
ORIGIN      
        1 aataagatac tactgtgtta atctgaaaaa aattacttta aagtagttac atactacctg
       61 ttaaattaga tactaccatt aatacttaaa ttagatacta cctatattta tattaagtac
      121 taccgttcta ggaataagat aataaaaaaa aactaccaaa ttgcaattaa atactactta
      181 aaatgtaaaa aataagaact acccaaatta ggtagttctt aataagagaa tatcagataa
      241 atttgtacaa gtcataaaaa aattaatctt aaatttttaa atatatatac ctgttaatta
      301 taaactactt tatttaggta gtacataatt aacagataaa actacatgct tgtagtttgt
      361 ctagctaatg agttgcatat gatttatata aaggtgtgca gcataactta gttgaagtta
      421 agagaataga ataaatattt tttgttttgc taataattaa acactaccta aatatggtag
      481 tgtttaatta tataatattc attttgttac aaataatcgt attataaaag cataacaatt
      541 gggataacta ttttttagat tatttaagta ctacttaaat taggtagtac ttaaattttg
      601 gtttaaggta gtaggtaatt aagaaataat atatatcttc ttttataata gaaggggaga
      661 ggcctagtta aaaacagcga caattaataa caatgtattt aatgcttcac aaaatccatt
      721 tggtggacaa tgcattgtta ttagcgatga tctaccgttc taagtagttt tttctattgg
      781 ttgcattcgt gactaattat ggtataatta tgagtaacaa agaggtattt cattatatca
      841 tcaaacccca tcagcgtgta ggctgatggg gtttttttta cgtttctata ttatagaatt
      901 aactggtagg cttactgtct tttttggcaa ttttgcaatc aagccacttg cagatgtagt
      961 gtgcagttac ctctgcaaat ggcttgattg caaaaacaaa atcaaaaagg tatttcatta
     1021 tatctcctcc tccccttgcc agattaggcc ggggtagcag acagcaaaac tattataaca
     1081 taactgaaat acctaaatga catatacaga tatgataatt tatgtattga tttttctaac
     1141 aaaagcacat attttaatat atactaccta ttttaggtag tatatatttt tagatacaat
     1201 atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctacataga gttactttca
     1261 atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt gatgaaattg
     1321 ctattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat tggtctaatg
     1381 gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa aataaagtta
     1441 ttccagtatt gctatatgga acacataaca atttttatga attagccgag aacactcgca
     1501 actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta tatcaatcaa
     1561 ccgaccgaca tatgttaaat ctatatataa taaaaatacc tacttaaatg gatgttaacg
     1621 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
     1681 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
     1741 gcaattgtcg ttataacatg ttataattta tttatcaaaa actaaaagaa gtgtgataac
     1801 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
     1861 tctcttggat taaaaagcgg agacactatc caagccaaac aagaaggcaa cttattcata
     1921 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
     1981 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
     2041 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt ccagaccagc
     2101 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
     2161 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     2221 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     2281 agctatgcct tattttatcg gatcgaccat gaaaaaaaga gatacttgta ggacgtattt
     2341 tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt cgctcatcct
     2401 ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta ctcgaagatt
     2461 gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag gtctaaagat
     2521 acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc gcttggtgaa
     2581 taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt cccaaaaatt
     2641 tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt gacgtaactt
     2701 ttaataaata ctacctaaat aaggtagtat ttatttttgt gttttaaaag aggaagtcca
     2761 atatatctat gggaaacttc ttaaatatta tgttggtaca tgaaaatata catccctgct
     2821 agactattta caacatttta ctttgtacgt attcttgttt taaaaggaat gggataaaat
     2881 ataggtacaa taactatttg atgctaaata taattaatgg aggagagata gtgaacatac
     2941 ttaatgaaaa aagatatgta tggtttgctg gtaaaaaata tattactact ttcaaaagat
     3001 tatccgagtt aatgcagcag actgttaaaa gtgctatgcc tgaaggatac aagtttaatt
     3061 ttgttcaatc ttcagtatct actgaaagta tctatttttc aattctttct gagagtaggc
     3121 tttttcattt tagaataagt tctcatgtaa agaaaactga tgttgatata tatacgttcc
     3181 gtacaaataa ttataaaaat ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg
     3241 ggaatagcta tctcaagatt cgattttcac atatattaat tttactacca tatttgaatg
     3301 agaatttcac cctaattcaa gtagataagt ctttcattgc ctcaggtaat caaggaaata
     3361 t
//
LOCUS       DAZUKT010000165         3318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000165 DAZUKT010000000
VERSION     DAZUKT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.173"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..754
                     /locus_tag="ACG816_003000"
     CDS             <1..754
                     /locus_tag="ACG816_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4921801.1"
                     /translation="WIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINT
                     WNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIID
                     GLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGID
                     KEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPD
                     SMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            744..1484
                     /locus_tag="ACG816_003001"
     CDS             744..1484
                     /locus_tag="ACG816_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4921802.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1481..>3318
                     /locus_tag="ACG816_003002"
     CDS             1481..>3318
                     /locus_tag="ACG816_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4921803.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1247 a          403 c          639 g         1029 t
ORIGIN      
        1 ttggatcgat ttaaaatata atgccattac tacttggaat aatattaaac agtttttcaa
       61 agatacttgg aaaaatatta aagatacggc atacaatacg tggatttcta taaaaaatac
      121 catgattaat acatggaata acattaagga ctctttctgg aatattgtta ctggaattgt
      181 taattctgct gaaaatgcat ggacaaatct aaaaaatggt gtttcaaatg ctattaatcg
      241 ggtaaaagaa atctttaatt cgttaaggga agtcaattta tttgaaattg gcaagaacat
      301 cattgatgga cttataaatg gtgtaaaaga aaagtggaat gctttgaaag atactatcaa
      361 aggtattgca ggtaatatca aaaattggat taaaggagct ttagatattc attctccatc
      421 aagatggatg cgtgatatgg ttggtaaaaa tattgttcaa ggtattatcg ttggtattga
      481 taaagagcaa agtaagttag atcaaaccat gacaaattta gtaaaaacac cgtctgttca
      541 acctgtagta aatggaataa gtcacctacc aattacacaa acaaagcaaa atatatctgc
      601 tagtgaaaca aaagaaattc atttacattt aaatgtttat ggagatttac ctgattcaat
      661 ggttcggaag attaccacaa aaattaaaac agaattgaca agacaaatga aacgagatgc
      721 tggtgcagta ggaggaacat tatatgcaac ttaagagagg acaatttttt attaatcaac
      781 attattcttc tgaatttaat gtgtatattc aaaacaggcc tgcttctgtt tcagctagtc
      841 gagtaattga attgagagag cgagaaggaa atgattctat cattattgat aaaggctatt
      901 acaaaaatgt gactagaaag attgagtgtt actataaagc accttcaatt gatatggtgc
      961 aggagtggga agatcgaatc actgaatggt tagatatgtc gtcttatagt gattttattc
     1021 tttactatga tgaacaatat atttatcaag ctgtagtaat agaaggacct gaatttaaag
     1081 gaacaagaaa aacaggaaat attgtccctt ttgaatttac agtgagtatt cgaccattta
     1141 aggaaaatta caatggtaga tttactattc gacaaacaga aacttttgaa atttataacc
     1201 cagagaagta ttcttcaaaa ccgcttatta aattgagtgg ttctggagat gcttcttttt
     1261 atattaataa ggataaatat gatttgaaat cgttggacag agaattattt atagattcta
     1321 aattagaaga ggcttatcga aaattagatg gtaatttaga acatcaggat caggtcacct
     1381 tatttttaga ttttccattt ttatatcctg gaaaaaatga aattaaatgg acgaaaaata
     1441 ttcattcatt tgaaataatg cctaggtggt ggagaaaagt atgaaaccaa gaatatatag
     1501 tcctactgaa acagatttct caacgaatgg cttaggaatt ttaaaggatt gtacaagatg
     1561 tggaatatat gaagtagcta atggaaaata tgaattagag ttggattatc ctttaggaac
     1621 tagatttgat gaatattttg aaaatgacta tcaaataaaa gcaaagccaa acgatcaaga
     1681 agagtatcat atctttttta ttgatgataa agatatagat acttttttaa atacagtaac
     1741 tatttatgct cagagtcgta caaatcgact tggaagacgg gtagtcactc ttgtggaagt
     1801 ggactctaaa actggtcaag aagcgatatc aattattgaa actaaaatgg ataaaaaatc
     1861 tgacatacga ctttattctg atattacggc tgtttctagc acaatctttg aagcgagaaa
     1921 tgttttaaat tgtattgctg gtgaacaagg atcattgctt caatattggg gcggagaaat
     1981 taaacgtgaa ccatttaagc tttctttgtt aaagcgaaga ggtcgcaata atgttggaac
     2041 gattcggtat ggaaaagata tgtctggttt gaaggtcaaa ttagattgga caggtataaa
     2101 aacaagaatt attccatatg ctgatcctca aagtgaagta ggtacgacta atcgaattta
     2161 tggttcgcca gtagatagtg cctatattaa taactatcct gatgtctata cagaacatgt
     2221 tcagtttaca gaagaacaag gagtaaaaga cattaagagc ttaaataaaa tagctaaaaa
     2281 ttactttaaa acgatcaatc ctggctgtga taagcctaaa gtttctatta cggttgaatt
     2341 tgataagttg actgatagtg aagaagcgaa agaatttgcg aagattagaa attatggtct
     2401 gtttgataca tttaaaatat atcataaaaa atataatatt tatcttgaat caaaggttag
     2461 tggactacaa tacgattctt tatctgaaaa aactttgaaa ttagaagcag gagacattca
     2521 agttgctttt tatcagcaac aagctgtaac tattcaggat aaattaaaag attatgcaac
     2581 gaataattat atgagtgatt ttaatgatta tgtttcttca atgattacag gtcaaggcag
     2641 tgcaggagga tatgtagttt tatggccaaa agaaaaacct tccaacatct ttattatgga
     2701 taatccagat ttagaaaagg caagagaagt actaagaatg aataaaaatg ggattgcttt
     2761 ttctaagaaa ggatggcggg ggccttttaa ttcagcttgg acattagata gtatatttaa
     2821 tgccaatttt atacaaactg gattaatcaa agcagatatc tttcaaaatt cttttaacaa
     2881 gactggagat gtattgaaat tagttaatgg actactccaa atttggaaca acaaaaagaa
     2941 aataatggag ttaactaaaa aaggaatgga attttggaat tctaagggtt caattggaac
     3001 gattggaaca actgattctg ttggtaatcc ttttcctggg gcttctactc caacccctat
     3061 tgaagataat tctttagtta ttcgtacaaa tggagacggc aaatatattt tgatttctcc
     3121 taaagtagga aaaggattag ttttattagg aaatggtaaa gcaatttatt ttggagactt
     3181 agatgtacaa ggaaaactta cagttaaagg aaaagaaata attcctggac aaggtggtgg
     3241 tccatcggga ggtggagaat ctactggtgg gtatcctagt gaattaaaaa cagatgcaga
     3301 aaagagagct tggagaat
//
LOCUS       DAZUKT010000166         3229 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000166 DAZUKT010000000
VERSION     DAZUKT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.174"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(553..2166)
                     /locus_tag="ACG816_003003"
     CDS             complement(553..2166)
                     /locus_tag="ACG816_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4921804.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="ACG816_003004"
     CDS             complement(2307..3215)
                     /locus_tag="ACG816_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4921805.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          556 c          572 g         1063 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctcttt
//
LOCUS       DAZUKT010000167         3228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000167 DAZUKT010000000
VERSION     DAZUKT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.175"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..1213
                     /locus_tag="ACG816_003005"
     CDS             173..1213
                     /locus_tag="ACG816_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4921806.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            2060..2392
                     /gene="traE"
                     /locus_tag="ACG816_003006"
     CDS             2060..2392
                     /gene="traE"
                     /locus_tag="ACG816_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4921807.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3011..>3228
                     /locus_tag="ACG816_003007"
     CDS             3011..>3228
                     /locus_tag="ACG816_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4921808.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
BASE COUNT         1195 a          451 c          593 g          989 t
ORIGIN      
        1 tagttcttaa tgactttaaa ggtagtacat aattcatttt atttctgatt ttaggtagta
       61 tttaattaaa aaacagtttt aatgcgtttg cattttttat ttatctttgc tattttcgaa
      121 aagagtaggt aattttacat aaataaaaca acgtgaaagt agaggatcta caatgactga
      181 ttttaatttt tttaaggcgg atagagttta caacgaatta ttttatcaat ttccaaaagt
      241 ctttattgtt tctgacgaat acaaaaaaat gaaagattca actaagattg cctatatgct
      301 tttaaaagca agattagaga tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt
      361 ttattttact tatacgacaa atgaactatg tagcgtatta aactgccaaa aacaaaaagc
      421 gatagcaatc aaaaaagagt tggaatcctt tggtttatta ttacaaaagc agatgggatt
      481 taacaaacag ttagggaaaa ataatcctaa tagactatat ctagcagaat taaaagtctc
      541 agaaaatgat atctacttac tcgaaaaatt tgatagagag aatagggaaa acgttgataa
      601 atcagagggt atgaaaatca tacccaccct cgacgaaaaa tcagacgctg aatcccttgg
      661 ggctcaagag ggtatgaaaa tcataccgtg ccaaaacgtt gataaatcag agggtatgaa
      721 aatcatacca gaacttaata ataatatatt agacactaat agacacaata tagacactga
      781 aaaagaccgc ctacaagatc aattgttgtt agacaatttt gagacaatta tgacaaacga
      841 cagcattgct acgtttatcc ctgaacgagt attaaattta ataaaaacat tttcttcaag
      901 ttacagtgaa gctcaaaaaa ctgtccagac tattcataat gcaaagaaaa aagccgaaac
      961 agaaagtggt atttcaataa tttttgaaga actcgatagt tattatgtca atgcagaaca
     1021 agaattatac acgacactgt taaaagccta tcaaaaatta aaaaccgaaa aagttgaaaa
     1081 tatccagaac ctgatttttg tctatgtaaa aaattggttt atcgaaaaac caatagctgc
     1141 taaagtatca agtgaaaaac gtttgaattc tgaaagcttc ccaaaaacta ttacgaaaga
     1201 ctggttagag tgaaatttca ctgacaaatt gattgttgct tcatggagta gcattaaaat
     1261 taaaaatttt ttattattct aattatatta caaatgttac aaagaaatag ctattttaat
     1321 agaatttatg agttaaaata ttatgataaa aatcacaaac gtagcttttt acgtcataag
     1381 tgttaatagt taataattaa tttccaacac aaaaaagaga acaaacgttt gcatgttgct
     1441 gttttttagt tataataata cgcgaagaga ggtaatttca ttttttaaaa aagctaaaaa
     1501 ccttgatatt acttcttttc aggcaatacg aatattattc gcttacattt ttttgtataa
     1561 atttagttgt tttaaattta attgtctgct acgcttgtga tgaattaatt ttcgttctgc
     1621 acttttggac gataaatttg gacaattgaa aatacaaaaa aaagaaaggc cctgtgagac
     1681 tcggaaggct ggtaaccttc gtcgacgtcc acaggtggca ttaagacaac ctttaatccc
     1741 ttaatgcgtt tacctttcag tgttacggta ttacccgcaa cggatttagt ataacagata
     1801 ttcaaaagtg ataccacttt ttttggctgt gttaaggtta aaaatacgtg atattacgat
     1861 gaatctatgc ctaagcataa tgttataaaa atgtccgtgt ggctctttgc cgtatgacga
     1921 acgctgaaat tagagggaac gcatgtttgg atgcgttccc tcttttttgt ttgtcatgaa
     1981 cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa cttattaaaa atatttaaag
     2041 taagtgggtg gattatttta tgtcatatac aggaattttg agtctcgaag atatttgtca
     2101 ctacggtaaa cggtgtaccg ctacagagaa aatcacgaag aaactatcaa ctggccaaag
     2161 taaaaccgtt gtgcagtgta agaaatatat aatccagaaa gacaaagtct cagaggaaat
     2221 gatttattac gctggtaaac aaaagcaaat aattttaaaa gatcctatcc ctttaaaaga
     2281 actatatcca acaatcaaac atgtttatga ccaaaacggt gtactgattg gtcgaagaaa
     2341 aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt ttaatcagct gaattgatca
     2401 ttctcataac ttgtatttta tcgtttttcg cttgcttgaa aatattgtcg agacttctaa
     2461 aagaacttag aattactaga aaaataagga gtagacttga tctatcaaca attcctttcg
     2521 gggggtgacg gggtaggttt ttacgattgg taacgtaaaa aattggtcaa tcgagtaaat
     2581 gcccttgttt ggatagtggt gaaatgaatt tgtgtaaata atatgcacag agtaaacaag
     2641 agggaattta gaagttagac aagttagaat agcgcctaga ttaatcatga gcagctacta
     2701 agggatctag gctttgacgc aaactcatgg aataaggagt aggtcttctc gtaatgggga
     2761 ggacgcacaa gaaattgtgt cgtgtgctgc agtacatgta gatacttgcg aggacgttgg
     2821 tcattggatc aatcgagcat ttaccgattt ctactagggt ttctcgtttc ggacgccata
     2881 aacgagtcga tattttttat gtatttttaa gtaaacgaaa aacgataaaa tatgaacgag
     2941 tcctgaataa agtgaaaaaa ttgatataaa acatagagaa aatgaaattt acgtaatgga
     3001 ggaatgacaa atgaaaaatt atgatccgaa tatacgattg ggcacacata ctattaaggt
     3061 ttctttccaa cgttgggatt acaaaggatt tgtgacgttt agaagaggcg gaaattgtaa
     3121 aggattagat gttttagcat tagacgagga tgatttatac tatcaaaaat tgactgataa
     3181 tccaattgga ttcggcttgc tacctgaaga tgatgaaggt aatgaatg
//
LOCUS       DAZUKT010000168         3199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000168 DAZUKT010000000
VERSION     DAZUKT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.176"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG816_003008"
     CDS             301..585
                     /locus_tag="ACG816_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921809.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG816_003009"
     CDS             588..1004
                     /locus_tag="ACG816_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921810.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG816_003010"
     CDS             complement(1141..1860)
                     /locus_tag="ACG816_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4921811.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG816_003011"
     CDS             2522..2608
                     /locus_tag="ACG816_003011"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4921812.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACG816_003012"
     CDS             complement(2910..>3199)
                     /locus_tag="ACG816_003012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921813.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAZUKT010000169         3185 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000169 DAZUKT010000000
VERSION     DAZUKT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.177"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG816_003013"
     CDS             complement(<1..132)
                     /locus_tag="ACG816_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4921814.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     "
     gene            826..1212
                     /locus_tag="ACG816_003014"
     CDS             826..1212
                     /locus_tag="ACG816_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4921815.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1310..1501
                     /locus_tag="ACG816_003015"
     CDS             1310..1501
                     /locus_tag="ACG816_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921816.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2015..2347
                     /locus_tag="ACG816_003016"
     CDS             2015..2347
                     /locus_tag="ACG816_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921817.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2257..2460
                     /locus_tag="ACG816_003017"
     CDS             2257..2460
                     /locus_tag="ACG816_003017"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4921818.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2491..2637
                     /locus_tag="ACG816_003018"
     CDS             2491..2637
                     /locus_tag="ACG816_003018"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921819.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            2644..2799
                     /locus_tag="ACG816_003019"
     CDS             2644..2799
                     /locus_tag="ACG816_003019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFS4921820.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         1159 a          530 c          493 g         1003 t
ORIGIN      
        1 atcataaaaa aaataagtag ttctatctga gcgatactct tttataatac tgaattcgga
       61 attaaaaacc acgataggta actttaaaca aacatgaaga gactttaata agacttcttt
      121 atctgaaaac acttttgttt ctctctttcc tttctacatc attaaatagt attttatacg
      181 ttatctaaaa tactattcct ctgtcaaaat aagctaactt acttcattat aaaatatcca
      241 cttatcgctt tcatgtatat tccaccgtaa aaagtacatt ttatcaccga gaactcatca
      301 tttttcatca tgatgaatac tatttacata tatctagttc aaagacaata attattatga
      361 tccgttatta tctaagcact atacagataa taggaatttt tcgtttctaa atactccaat
      421 ttgggaatcc aataaaaatt ttcgttcaaa tacgtgtcta ttcgaactct tttctagttg
      481 ttaattatga agggggaaga aataaaaaat acaatctata cacgaataag aatattatcc
      541 aaaaggaggt tttagaaaag atttattata cgtaaaaagc tcaagacatt ttcttagcca
      601 taaggacatg tttgatattt aagaagatat atgaattttc ttttctaagt ataaagataa
      661 ctatccatta tcaactaaaa aaacattttt gaaaataaac atcagcagtc ctatcaatgc
      721 atgtgagctt atagaagtta ttcgcagcgc agcataattg cattagcttc ttttcgtgtt
      781 ttttgcctct tttcccttta aatcttaatg gaggaggtgt gcaatatgtc agataacgac
      841 gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg tcattttgaa caccgctaat
      901 acttattata aaaaacaaga agaaattcaa aaacatgaac aacttgatca actcccacca
      961 tatttgttta ctaaagatac tataaaattt aaacaaccga ttaatgtttt aaatgtgact
     1021 ctccttgttg aagatataaa attagcaaaa atccttcctt tgttgaaaga acctgaacaa
     1081 atttttctta tcgaaaaatt tatctttaat aaaactgata aagaaattgg aaaggttctg
     1141 ggtattacaa gacagggtgc gacaaattta aaacatcgac tttacaaaaa aattcaaaaa
     1201 atgctggttt aatatctgca taataactaa ttttgtatgc ttctttgctc gtcctcctcc
     1261 ttccccaaaa aataaataga ttataagttc atgggaagaa ggtaaattta tgaatacttt
     1321 aaaagaagat ttcattttag caaagaatgg aaatgaagaa gcagttgtgg ccattctcaa
     1381 aagatttagc gctttggtcc ataagcaatc ctggagaact ggaaaatatg atcaagattg
     1441 ttatcaagaa tgtatgcttg caatatatct agctatctcg aaattcgaaa ttaaagaata
     1501 actcaactaa gggagtctgt gacataagta aaaagatagt ttcaaatttc gaacaataaa
     1561 tttgaagcca tcttctttat aattataaag tatcgatttt acagcgttct ataatgattc
     1621 atagaatcta aaacgaatga ttttaattat taacgttttt taattcgttt ttagcttaaa
     1681 aataactttt gtcacagtct cactcctaaa attactttat ttacgtacga agtgggcagg
     1741 aggtcgaatc cagaactggg ccatggtact tcaacgagga cttctcctct agaattgaaa
     1801 agcatgcgat ttaccttccg taacgacggt agatttattc aactatgtgt ccaatcaaaa
     1861 tttgtctgat ttcaaaaata gtcaagagaa ttttggttgt taaaaactct tatctatcaa
     1921 cttccacagt ttagttgaca aaccctgata ctaagttggg ctatgataca cttttcttga
     1981 ctatcagtta cgttaccgga aggaatgaac tctattgaaa aaaacatatg atcgtaaatt
     2041 taaactaaag atttcacagg atatccttga gaaaaaagta actaccaaga aaattgctga
     2101 agaatacaat atctctcgtc caacaatttc ccgatgggtt tcagaatatc gtcgatacgg
     2161 gaaaaatgct tttgctggac aaggaaaaag attacctgat aaagcagatt tctacattct
     2221 tgaacaggaa aataaacgac taaaagagga agacgagtgt tctacgaaca caaagatcga
     2281 tacggatctc cagggatcac tcaaaaactt tacgatgaag gaatcgaaat caataaacgt
     2341 gttgtagctg ttcttatgcg tgaaatgaat ttatgcacaa gaggatttca tcgacgcaaa
     2401 tcttctcatg gtagagaaaa aacaatcaaa gaaatggtaa aaaaaatgtt gaatcgataa
     2461 tttgaacaga accaaattga tgctgtttgg gtgacggata ttacttacat tccatgtaat
     2521 gacggtcgcc tctatttgtc cacctatatt gacttagcaa ctcgtattct acgttactat
     2581 atggtggatt cacacatgaa aaaagagatt gtcgtccgtc cattacaaat ttattaagga
     2641 gaattgccta cggtaattca ttccgataga ggtagccagc atcggtcttt tatttatcaa
     2701 gagctgttga ccgaccacca catcacacat tcaatgagcc aaactgggac tcctgttgac
     2761 aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag aattaatcga ctccaacaaa
     2821 tataaaagta aaatagagat gaaggtgtta atccaagatt atttagaaga ctactaccca
     2881 aacaaaagaa tccatacaaa atttatgatg actccttgac aatttgaagc gaagaaaata
     2941 agggatctat aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc
     3001 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
     3061 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
     3121 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
     3181 ttgtt
//
LOCUS       DAZUKT010000170         3079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000170 DAZUKT010000000
VERSION     DAZUKT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.178"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            468..1640
                     /locus_tag="ACG816_003020"
     CDS             468..1640
                     /locus_tag="ACG816_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFS4921821.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1770..>3079)
                     /locus_tag="ACG816_003021"
     CDS             complement(1770..>3079)
                     /locus_tag="ACG816_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFS4921822.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT         1054 a          553 c          399 g         1073 t
ORIGIN      
        1 aaaaagttca aaagatcaag tgtgcgcttc gctcccactc ccctcgacgt tttagtagcc
       61 tttccctcac ttcgttcagt ccaagccaac taaaaagttt tcgggataca ttctccttct
      121 cttcctaata atcaattaaa attttaatct ttatatttct gctaatcact caggaaacag
      181 tacagaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg ggttttattt
      241 tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gtttttgggc gttctgctag
      301 cttgtttaaa gtctcttgaa tgaatgtatg ttctaagtca aaaaactctg acagcgcctt
      361 tatatagata aagtccgtat aattgtgtaa aagtaaaaag gccatataac agtcctttta
      421 cggtacaatg tttttaacga caaaaacata cccaggagga cttttacatg acccaagtac
      481 attttacact gaaaagcgaa gagattcaaa gcattattga atattctgta aaggatgacg
      541 tttctaaaaa tattttaaca acggtattta atcaactaat ggaaaatcaa cgaacagaat
      601 atattcaagc aaaagaatat gaacgaacag aaaaccgaca aagtcaacga aatggctatt
      661 atgagcgcag ctttacgaca cgtgtaggca cgctagaatt aaaagtaccc agaacacgtg
      721 atggccattt ttcacccaca gtgtttgaac gttatcaacg aaacgaaaaa gccctcatgg
      781 cttcaatgtt ggaaatgtat gtatcaggcg tttcaactcg taaagtatca aaaattgtgg
      841 aagaactttg tggtaaatcc gtctctaagt ccttcgtttc tagcttaaca gaacagctag
      901 aacctatggt taacgagtgg cagaatcgtt tattatcaga aaaaaattat ccttacttaa
      961 tgaccgatgt actctatata aaagtacgag aagaaaatcg agtactctca aaaagctgtc
     1021 atatagcgat tggaataacc aaagatggcg accgtgaaat tatcggcttc atgattcaaa
     1081 gtggcgaaag cgaagagacc tggacaacat tttttgaata cctaaaagaa cgcggtttac
     1141 aaggtacgga actcgttatt tctgatgcgc acaaaggatt agtctctgcc attagaaaat
     1201 ccttcaccaa cgtaagttgg caaagatgcc aagttcactt cctaagaaat atctttacca
     1261 ccattcctaa aaaaaattca aaatctttca gagaagctgt taaaggaatt tttaagttca
     1321 cagatattaa cttagcgcgt gaggctaaaa atcgattgat tcatgattat atcgatcaac
     1381 caaaatattc aaaagcttgc gcatcattgg atgatggatt cgaagacgcc tttcaatata
     1441 ccgtacaagg aaattcccac aatcgactaa agagtaccaa tctaattgaa cgactgaatc
     1501 aagaagtacg cagaagagaa aagattattc gcatcttccc caatcaaaca tcagccaatc
     1561 gcttaattgg agccgttctt atggacctac atgatgaatg gatttattct tcaagaaaat
     1621 acatcaattt tgataagtag aaatggtaaa aacattgtat agcattttac acaggagtct
     1681 ggacttgact cacttccttt attatttttc atttttttaa actatttaat actaatgtct
     1741 tttataatag cttttcatat attatataat caatctttat aagtcctttt ataaatttct
     1801 tttctaccat tttcgataaa ttcctgttta atatttttaa ttccataaac aatagtttca
     1861 ataggataat attcttcaac tatatcttga tattcttttg ctttctcaat atctatattt
     1921 ccatacattc ttaatatatc ttctccaaaa tttgttccta tttcttcttc actatcttca
     1981 agtaagtata taaaatcaca atattcatct ataattccag aatctccaaa atcaattatt
     2041 ccagttaatc tattattgcc atctaacaat agatgattac aactaaaatc attatggcat
     2101 aaacactttt taccctcaaa aactgttgtt gcatttagtc tttccataaa actttctata
     2161 taatcttttt ctatatcagt taaatcatta taaatagttt cacgcaacaa tatatactct
     2221 tctaatacat tttgtttatt atcaatagta cattcactaa tatctgtata atctaaaccg
     2281 tgcatttgtc ttaaaaaact ggcaatatct cgttttaaca aattttgttc ttcttctgac
     2341 atagtagaat aaatttctgg tgttaaaaaa gttcctttaa tttctttata acctagtata
     2401 gataattcat cactaatata cgaatattca atattaggaa tttttacatt agtttctaaa
     2461 tttgtattta aaaaattata tattgctttt tcttttgcat aacctttttt cttattagta
     2521 ctaaattttg ttttaaaaat gtattcatta ttaactaaat atgccacact atcataacca
     2581 ctaccgatta tttcaatact atctactttg aaattatcaa agtaatgctc aattaaatat
     2641 ttcattgcct taacatttgt ggcattatca tcatatctat attccattaa ataacaatct
     2701 tcttttttgc cctcgtgtaa ttcatgttct ggcaaatctt caataattct aaaaccagat
     2761 ttttggtatg cccttattgc tcttggatta tttttatgag ggtctaaaat aactgcatta
     2821 gcatttcttt cttttttcaa aaattcaaaa atcaatttaa tatatcttgt accaattcct
     2881 ttactccaat aatttggctc tcctataaat tgatccatac catagactat ctcatcagtt
     2941 tttggataat gataatcagt atataactca tcatacattt tatatatttg tccatatcca
     3001 ataggaacat tgttatattc aataattact ctaaaaactt catcttccca aggctctgta
     3061 taatgttttt ttaatgatt
//
LOCUS       DAZUKT010000171         3043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000171 DAZUKT010000000
VERSION     DAZUKT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.179"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..1224
                     /locus_tag="ACG816_003022"
     CDS             256..1224
                     /locus_tag="ACG816_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4921823.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1217..2248
                     /gene="vanA"
                     /locus_tag="ACG816_003023"
     CDS             1217..2248
                     /gene="vanA"
                     /locus_tag="ACG816_003023"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4921824.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2254..2862
                     /locus_tag="ACG816_003024"
     CDS             2254..2862
                     /locus_tag="ACG816_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS4921825.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(2951..>3043)
                     /locus_tag="ACG816_003025"
     CDS             complement(2951..>3043)
                     /locus_tag="ACG816_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4921826.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          900 a          584 c          733 g          826 t
ORIGIN      
        1 ttccagcgat gccagacttg gttgataaaa ggaggtccta agagatgtat ataatttttt
       61 aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa
      121 acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag
      181 agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa
      241 gggttggtct taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag
      301 gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc
      361 aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat
      421 aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt
      481 tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc
      541 actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt
      601 cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg
      661 ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc
      721 cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat
      781 agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc
      841 gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa
      901 caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta
      961 gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat
     1021 gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat
     1081 caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat
     1141 tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt
     1201 gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga
     1261 ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa
     1321 atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc
     1381 ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat
     1441 gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt
     1501 ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc
     1561 cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt
     1621 gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa
     1681 agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc
     1741 aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga
     1801 atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt
     1861 cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg accaaatcag
     1921 gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa
     1981 cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac
     2041 ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt
     2101 acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata
     2161 cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg
     2221 cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat
     2281 gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga
     2341 aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg
     2401 cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt
     2461 taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac
     2521 ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac
     2581 gtggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta
     2641 gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat
     2701 catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc
     2761 atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac
     2821 gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg
     2881 gacaaactat ataagcggtt ctgttgcaaa gttttaaata aagaataaaa tcccttacgg
     2941 tatctatgat ttaagctggg attcccaata ataccttgat ttcagtacag accgaaaacc
     3001 cgaagagagt gccttctttt cgggttttct tatataatcc tcg
//
LOCUS       DAZUKT010000172         3010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000172 DAZUKT010000000
VERSION     DAZUKT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.181"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..358
                     /locus_tag="ACG816_003026"
     CDS             176..358
                     /locus_tag="ACG816_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4921827.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            461..745
                     /locus_tag="ACG816_003027"
     CDS             461..745
                     /locus_tag="ACG816_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921828.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            761..997
                     /locus_tag="ACG816_003028"
     CDS             761..997
                     /locus_tag="ACG816_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921829.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1019..1273
                     /locus_tag="ACG816_003029"
     CDS             1019..1273
                     /locus_tag="ACG816_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921830.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1355..1540
                     /locus_tag="ACG816_003030"
     CDS             1355..1540
                     /locus_tag="ACG816_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS4921831.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1607..2002
                     /locus_tag="ACG816_003031"
     CDS             1607..2002
                     /locus_tag="ACG816_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4921832.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            2019..2369
                     /locus_tag="ACG816_003032"
     CDS             2019..2369
                     /locus_tag="ACG816_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921833.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            2391..2765
                     /locus_tag="ACG816_003033"
     CDS             2391..2765
                     /locus_tag="ACG816_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921834.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          922 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaacat caccatctga tccaaaacag
      121 ctcagaaacg catttaaacg tatctgatac caatattaca gaaggagtgt atcgtatgat
      181 taacctattt cttttagtga cagctagtac attattcttt actggaatat cactgataat
      241 ttcctatttg atctataaga aagccgaaaa tcacacaagc aaagcaatcg gactagccgt
      301 ttcatccttt ttaactttgc tgactttatt tgttacttta tcgttttctt ttgtttagaa
      361 cagctgatcc attagcacct ttttctcttt acattataca cactgttgtg tataatgtat
      421 ttgtaaacaa gctaattaac ttttaggaag gagtgacaag atgctaacag ctactttaca
      481 gcaacctact cacgttgcta ttgcagaaag ttatacaatt aatgcaggga tcgttaaaca
      541 gctgatcaag gatcgagaat tcaaaaagtt taacagtcgg caagcgtata acagacagct
      601 aaagaaagct attctaaagc acaaagattc cttcatcggg aacttggaaa gtgtagatgt
      661 taaaactgtt tcaacggcat tttcagttgt tgaagtgact attgacggtc gcttgatttc
      721 tctatttgct tttggaatta attgataggg agtgtgaact ttggaaggta agacttttgc
      781 agaaatttgg actgaattta ttgaggtacc aactcgcatt gatccgataa cttctgaaaa
      841 ggtattggat gcagacttct acaatctcaa agcaggtact agccttgaat gcgtttggat
      901 gtatttagaa gagcatttca aaggcgtaac gctcggtcac gtcttatacc acaatggtgc
      961 cgagtgggat gcaattggaa atatccattt ttcttaatat aagaaaggag aacacaatat
     1021 gaattatacc gatgaagaac aaatcgcttt ggaatacgtt ttcacaaatg aagaagggga
     1081 ggaaattgaa aaagtcttga tcatttcatc aaaagacttg gagaaggctt tggcttatga
     1141 accatacgaa tcaaccgtaa ctgtcattga attcttagat acctatactt ctaatgatac
     1201 ttgggaaatt atgaatattt gcaacgctaa taacattcaa tttgctgaaa aaaccaataa
     1261 atcattttat tagttttgca ataaaacatc ctctttttct attgattcca tacacaacaa
     1321 tgtgtataat agattttgta aagggagggt agctatgcct aagacagcaa gagaaattgt
     1381 aaaaattctc aaatctaacg gcttcaaaaa gactggacaa actggcagtc atgcaaaatt
     1441 ttataattcc aagactaaaa gaacggtcgt tgttccgatc cacaatggtg atgtaccact
     1501 aggaacagaa gctaatatct ggaagcaagc agggctttaa cagccttgcc ttcctatttg
     1561 gtattaggca tactatcctc tcttacacta gaaaaggagc gaggaaatga agacaaaaaa
     1621 tattttttat ccggcaatct tcgagaaaga aggagaagct tataacatcg ttttccctga
     1681 cttaccggaa atatctacct ttggaaatag tcttgaagaa gcatatttaa acgctaagga
     1741 tgctttagga ttagcgctat atagtgtacc agatgcagaa tttcctaaac catcagcagt
     1801 ggaagaatta actttgaaag ccaaccaagt ggtagtaatc atccaactaa acattaaact
     1861 tttccgaaga agcttaaata caaaaacaat tagaaaaaat gttagtgttc cggaatggtt
     1921 agttctttta gggaaggaga aaaacataaa cttctctcaa ctacttcaaa aagcactaga
     1981 agaagagttg cttgaaaagt aaaaaggaga ccgaacctat gaaagctaca cacttaaact
     2041 atttctctcg aacgttaatc aataatctat taaaagatac aactggcatt agcgttagta
     2101 ccaaacactt tgccaatgat ctaattattt ttgaggataa taacttgcga attgagatgt
     2161 ttattgaaca ggcaacccct aactcaatac ctgaattctg ctatacgcac actgaaaaaa
     2221 tgaaacatgc aaaatcagtt aaagatgttg aagcattaag tgaatcacta acgaacgctt
     2281 taaacctcgt taagaaggca aaattgttat ttgaatttca aaccgactac cacaatgagt
     2341 tgcttgctca atctgaaatt ttagattaat tgaaacagga gaagagaaag atgaaacaaa
     2401 cacaaacaga aaccgttaac gatacgcttg ctgatcaatt agcaggttat tataaattat
     2461 ttgagatggg taaagcacag cgcttgatcc actatctttg gaaatacaaa attttgtttc
     2521 ttttttggga atcaacacta acattctcag ttctttttta taagtcagat ttttataatc
     2581 aacacttagt tttagggagt tttttgctta tctacaccat tttgatgctt aatttatata
     2641 cctacatcac acttgaagaa cacttagtaa ttagcgatgc agggatcgag agaaacatca
     2701 aaagggtatc cttcaagatc acgttaaaca agcctaccga taaaaagctc tcagaagcgt
     2761 tctgagagct tttgacattt tcttgtataa ttttatccta acacattaca aagcacctaa
     2821 ttcgagctta tatcaagcaa atttgctatt tattactcaa tacatatcta aaaattactt
     2881 gatggaaaat tagatacatg gtaagcatga aaagaaaatc aatttgagca gatgcagtac
     2941 aatacatagc aatttaaaag ctctcagaag gcttctgaga gcttttaata tattctttga
     3001 taatttgtct
//
LOCUS       DAZUKT010000173         3001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000173 DAZUKT010000000
VERSION     DAZUKT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.182"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG816_003034"
     CDS             <1..963
                     /locus_tag="ACG816_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4921835.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACG816_003035"
     tRNA            1152..1225
                     /locus_tag="ACG816_003035"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
     gene            complement(1740..2039)
                     /locus_tag="ACG816_003036"
     CDS             complement(1740..2039)
                     /locus_tag="ACG816_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4921836.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2045..2275)
                     /locus_tag="ACG816_003037"
     CDS             complement(2045..2275)
                     /locus_tag="ACG816_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921837.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(2534..2647)
                     /locus_tag="ACG816_003038"
                     /pseudo
     CDS             complement(2534..2647)
                     /locus_tag="ACG816_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1019 a          509 c          519 g          954 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt ataattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
     1621 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
     1681 ctcctttcat aagtatggtg ataaaatccg ttccgggcta cctttttagg tagcctactt
     1741 taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
     1801 tcgcttcgag cgtctagcac atcagtcacg atatcaaaca aaaaatatac gtctttcttc
     1861 attctagttt tcgcagcagg gattttaaag tagttcttat tagaatagta gagattaatt
     1921 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
     1981 ttgacaaatc tattcgaagg atcaaattta aaccataatt gcgtctcatt aaaaagcata
     2041 accattactc cgatctattt ttaaacttag tctctacctc taatatatat cgagttttta
     2101 ttttgccttc agagaatact gtttcttttt ttgcagttac aggttgttta ttttcggaaa
     2161 aagctaatat agctaaaatt gaaacatcca tctggaattt gtctttttcg ttgctttgct
     2221 cataaaaatc tgcatattca tcactaatat tttttctaat aaattcttcc atcataaaga
     2281 tcacctcgaa aagagtatac gaacaaacgt tctttttgta aaggtggaat ttattatact
     2341 aaataaatag gtgaacaagt acttgtgcca aattatgtgc caaaaaaaat cgaatttaat
     2401 aaaacacaaa caaaaagaaa tcctattatg ataacatttc ttataataac aaacacaaca
     2461 gaagacgtgt aatagttagt caggaacgta caaataaccc ctgtatcctt tgcggtacag
     2521 ggggttattt tgtataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
     2581 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc
     2641 catcaataaa cagtcgaaga gaaaataggg aaaaattgat tactaaagaa aaacttacta
     2701 ggtcgacggt gtagttgatt ttcatacttc aatagattcg aattaaacga tttcgattgg
     2761 cttataccag tcctatccaa cgaaagatat atcgttctta actctagcgg tataacttga
     2821 agctttacgt aaagccaatg ggaatgaact ggagatgact ttttattatt tttagtaata
     2881 gctgatgggt taatacttac aactttcctt ttcaaagtac catataaaaa aacggtgggg
     2941 ttgtttagcg aaataaagga tctaagccaa tagcttagat cctttattct atgctttata
     3001 t
//
LOCUS       DAZUKT010000174         2998 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000174 DAZUKT010000000
VERSION     DAZUKT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.183"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG816_003039"
     CDS             <1..227
                     /locus_tag="ACG816_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921838.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG816_003040"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG816_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG816_003041"
     CDS             1635..1841
                     /locus_tag="ACG816_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921839.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG816_003042"
     CDS             1862..2458
                     /locus_tag="ACG816_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFS4921840.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1094 a          404 c          588 g          912 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatca
//
LOCUS       DAZUKT010000175         2808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000175 DAZUKT010000000
VERSION     DAZUKT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.185"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..262
                     /locus_tag="ACG816_003043"
     CDS             <1..262
                     /locus_tag="ACG816_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4921841.1"
                     /translation="WIFDRSRNSSIGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSS
                     TLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(353..718)
                     /locus_tag="ACG816_003044"
     CDS             complement(353..718)
                     /locus_tag="ACG816_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921842.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            926..1408
                     /locus_tag="ACG816_003045"
     CDS             926..1408
                     /locus_tag="ACG816_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921843.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1605..2495
                     /locus_tag="ACG816_003046"
     CDS             1605..2495
                     /locus_tag="ACG816_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS4921844.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          957 a          447 c          550 g          854 t
ORIGIN      
        1 atggattttt gataggagta gaaattcaag tataggtttt agaactaaac gaagtttgtc
       61 ttctaaaaaa aattgggtat actcacaaac tattttttat ggaggaattg ttttaattag
      121 tttactttct tcaacacttt attctttgaa tattatagat gtttccacct caaattcaat
      181 atctattatt ggaatcatta ttgctgcaat tattacacaa ctgtttttag tgtttggaga
      241 gaagaaaaga agtaaaaaat agaaaaacta gtgatagttt tggtgatata agaaaaacat
      301 atatgtataa aaagaagaaa gtagcttctc tttgtacata tatggtttgt ttttatggat
      361 gccgagcttt tttataagat aaggctaccg cccataacga cccaaggatt tgtggaacta
      421 agtaaatacc tgcaagaagc aagatcagct gggtcggttt taaaggatga tagaagtaac
      481 gccattcagt aaaattggcg caaaataaat aggaagtgac agtagatata atattggcaa
      541 tgaataaaga tataatacgc ctgttccttt gaaaataata tccagcaaaa agagataaaa
      601 atatcaatgt gatgaacccg cttacggaat gataacgaaa atcaagcagt aaggcgaaat
      661 gcacataagg aacaaaagat aaaagcaacg gaaggttttt ccagcaagtt tttatcatct
      721 ttcatcacca cctaacaagt gttattattt tgttagtttt attgtaaatt gaatcctgca
      781 ataatcaatt actttttcat tatttctaga ttaaatgaat aaactctcat gccattatct
      841 ctaaaaaaaa agtttttccc cctaattttt ttagatagaa aattgtatag tatgatgaga
      901 gaaaaagtag tttgggggaa cgttaatgag aagaaaagca gtaatcgatc ggctttattt
      961 atttttttgg ttttttccta tcgttttgct accttttaca tgggtaaatg ctgttgtaca
     1021 aggtgcggag atattaggcc tgatccaagg aagcggattt gtggtttcta ggaattttcc
     1081 agctttgatt gttttctatt tagtgataag tgtaggtctc ttattaccaa aatctctgat
     1141 caatcactac aattggcagc tgctttactt ttcttgctta ttagtattta catctacgtt
     1201 ttctttgctg cctcgcttga ttatcggccc taatggtttg agcgagagtg gattgaagga
     1261 tttagcttat tcctccatgt atttcttgac gatcaaacca tggttctatt tagtaattgc
     1321 ctcatttatt cttgcaacat ttttttactt ctggacagaa atcaggataa aaagtgaacg
     1381 atctgtatct ataaaaaata actattgatg gtggatcatc agtaaaaaaa catatgagct
     1441 gcgtaaaatg catgtttctc atatgctttt tttattatta tgtaaagaat gcgaaaaacg
     1501 tttacatgat ttttacataa cttaactata tttttacagc aaccatacga gggatttaca
     1561 gtcgactgat aaactttagt tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt
     1621 ttactctagg tatgatggga attcttttag ccggatgcgg ctcaggatca ggaaatacag
     1681 atggctctgc cacacaaaat aattcaagtg actcaaatga acaagtaaaa atcgttgcgg
     1741 taggttcgac agcattacag ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa
     1801 atccaaatta tcaaatctct gtacaaggag gaggaagcgg aactggtttg agccaagtcg
     1861 aagctggagc agttactatc ggaaactctg atgtatttgc agaagaaaga gacggagttg
     1921 atgcttcaaa attagtggat cataaagtag cagtggtcgg gatggcacca atcgtcaata
     1981 aagatacaga tgtcaaagat atcaccaaac aggaattgat tgatatcttt acaggaaaaa
     2041 tcactaactg gaaggaagtt ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa
     2101 acggcagcgg tacccgcgcg acattcgaaa aatggggact ggatggtgct acccctgtgc
     2161 agtcccaaga acaagattca tcaggaacag ttcggcagct ggtaagtcaa acaccaggag
     2221 caatcagcta tttagctttc tcatatctcg atgattccac acaagcacta agtatcgacg
     2281 gtgtagaacc aaaagaagag aatgtagcag ataacagctg gggaatctgg tcatatgaac
     2341 atatgtacac gaacggaaaa ccttcccctg aagtccaaaa attcttagac tacatgatga
     2401 cagaagaaat ccaagaagga cctgtcaaag aattgggcta tttaccaatc acaatgatgg
     2461 aagtagaacg tgatcacgaa ggaaatatca aataactagc agataaacat gccacggtag
     2521 tcgttactat taaggaaaaa tcaactatgt ttccccacat atacattttt cctttaattg
     2581 tgaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg
     2641 aacaccgttt attcgaaaaa aagagctacg acatagaact gtcgtaactt tttttggctc
     2701 ttcgtcaaat cgtgttggtg aagacaaatt tgaataaaac ttagaccgat atgcggcgcg
     2761 ttctgtgcgc cgcatatttt tttagttttc aaagatcaga ccttgttg
//
LOCUS       DAZUKT010000176         2796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000176 DAZUKT010000000
VERSION     DAZUKT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.186"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACG816_003047"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACG816_003047"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4921845.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DAZUKT010000177         2680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000177 DAZUKT010000000
VERSION     DAZUKT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.188"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1355)
                     /locus_tag="ACG816_003048"
     CDS             complement(12..1355)
                     /locus_tag="ACG816_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4921846.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(1489..>2680)
                     /locus_tag="ACG816_003049"
     CDS             complement(1489..>2680)
                     /locus_tag="ACG816_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4921847.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT          758 a          513 c          354 g         1055 t
ORIGIN      
        1 tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat aaaattatag
       61 ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc taatgtaatt
      121 tgttcttcta tagcaagaga aaacattgat aatagtccag ataaatcttc ttgactacaa
      181 agttgtacac ctactattct ctttgtattt ttttcaaagc taattcttaa ttttacacga
      241 ctattttttc tcaaaaaaac aggtctaatg aaatcttcta tttctatata atctatagac
      301 tcacttaata atcgtgctgt ttttaaattc aaacctgctg cgtaaaaatt gtaattaaat
      361 atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa aatcttgatt gtcaatatgt
      421 tgagttgcaa tataagctgt tcttagagca ttagaaatag aaaaatcaac actacttcta
      481 ttaatggacg taaaaaaagt tgtagcacaa tccccaatag catatatatc ttttatattt
      541 gtttgctggt attcattagt tagatatgct ccactaacat ctctcaagag ttcctttttg
      601 ccaattggac tattagatag aaatcctata gcttgtacga ccatatccat agagtattgg
      661 gtaccacttg tagtgactat tttttcaatc ttttttcttc ctaaatattt tttaggactt
      721 tctgatagac aaagatgtat tccatttttt gatagtattt cttcaacttt atctgaaaaa
      781 ctacggtcgt aataagtcga caacactcta tctgcaacat cgaaaagata tacttctttc
      841 cctctttttc ttatggcttc agcaatttca actccaatat atcctgctcc aataattcct
      901 acttttttaa tagtattatc atctaaagct ttaaaaatct ctttagcatt tggaaaattc
      961 tttatcgaaa aaatattagc taaattattc cccggtacat ctaacataat ttgctttgaa
     1021 cctgtagcta gtataagttt gtcatattct tgggaaaatc ttgtgccatc tttttttaca
     1081 caaataatct ttttattttg aatatcaatt ttgtcaactg ctgtctccaa aaacaacttt
     1141 tttatcttag aatttagttc tcttctattt tgataaaaaa gatcttctaa attgctaact
     1201 tctcttttta tgaacaacga tgtcccacat tctaagtaac taatttctga ttttttatct
     1261 atcagaataa cttcataatt tgaatcacat aagtttagaa atcgagctgc agctagcccc
     1321 gaatggtttg atccaataat tactattttt ttcacttaat cacattcttt tcccttctat
     1381 gaaaagtatc ttcattttta actaacaacc tatctcccaa aataaaaaac aaaaagagga
     1441 agaaaaagat tcttcctctt ttcagagaag atttaagaaa ttgattttct attcattctc
     1501 tttttttcga tttttatacc ctaataaccc taaagttcct aacaggatta aaccaccact
     1561 aatagtaaag atattactat caatctctcc cgtcttagga taagcttttg aggactgctt
     1621 ttgaggactg cttttgcctc tatttttgtt ttgcttgcta ccttgattgt cagtcttatt
     1681 atctttacta acggcttttt cactgtcttt ttcgtttgaa gaaacaacta cattagtagg
     1741 ttcactagta ttaccagatt catcagttgc agttactatc acttttgttc ctgcctctaa
     1801 tttaggaact ttaactgtaa aattaccatt tttatcagct gtcccatggt attgactccc
     1861 gtttggtaat gtaactgtca cctctgcatt tggttcagtt ttaccagtaa caatttggtc
     1921 accctctgta ataggatcaa cttgaggttt attcggtgct tttttatctt ctacagaaat
     1981 agtaacctct acttcttctt tagatccatc tggatatgtt actactacaa tccctttttt
     2041 gtttccagct gtactaacat ctactttctc tttccaagta taagtagttc cttttggtag
     2101 atcttctttg ttcttaattc cctcagatgc gtctggctct ttatttaagt ctgtagtcac
     2161 tttttgtcct tcaggattgt atttatcggc atcggaacga ttgtctgtca cttctactgg
     2221 aactttcact gtatctttcg taccatctgg atacgttact tcgataaccc cttcgtaatt
     2281 acctggtgtg ttggtatcaa tcgtaccatc aggagtgaca tctgttactg ttgttccttc
     2341 tggtaatgtt ggcaagttag taacattatc tgttagatct actgtgcctc caacttctac
     2401 tttttcgcct tctactgttg gctcatattt atcggcgtca gaacgattgt ctgtcactgt
     2461 aacatctact tcaacttcat cagatgatcc atctggatat gtcactacaa cagtcccttt
     2521 tttatttcca gctgcgctaa catctacttt ttctttccaa gtatacttag tatcttttgg
     2581 taaatcttct ttgttcttaa ttccctcaga tgcgtctggt tccttattca attctgtagt
     2641 cactttttga ccttcaggat tatatttgtc agcgtcagat
//
LOCUS       DAZUKT010000178         2656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000178 DAZUKT010000000
VERSION     DAZUKT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.189"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..587)
                     /locus_tag="ACG816_003050"
     CDS             complement(117..587)
                     /locus_tag="ACG816_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921848.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            674..1372
                     /locus_tag="ACG816_003051"
     CDS             674..1372
                     /locus_tag="ACG816_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4921849.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1550..1891
                     /locus_tag="ACG816_003052"
     CDS             1550..1891
                     /locus_tag="ACG816_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921850.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1884..2555
                     /locus_tag="ACG816_003053"
     CDS             1884..2555
                     /locus_tag="ACG816_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4921851.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
BASE COUNT          980 a          386 c          534 g          756 t
ORIGIN      
        1 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
       61 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
      121 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
      181 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
      241 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
      301 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
      361 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
      421 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
      481 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
      541 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
      601 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
      661 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
      721 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
      781 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
      841 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
      901 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
      961 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
     1021 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
     1081 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
     1141 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
     1201 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
     1261 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
     1321 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
     1381 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
     1441 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
     1501 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
     1561 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
     1621 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
     1681 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
     1741 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
     1801 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
     1861 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
     1921 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
     1981 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
     2041 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
     2101 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
     2161 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
     2221 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
     2281 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
     2341 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
     2401 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
     2461 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
     2521 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
     2581 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
     2641 ggaagtgaac attgat
//
LOCUS       DAZUKT010000179         2400 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000179 DAZUKT010000000
VERSION     DAZUKT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.190"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG816_003054"
     CDS             207..2069
                     /locus_tag="ACG816_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4921852.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG816_003055"
     CDS             2069..>2400
                     /locus_tag="ACG816_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4921853.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZUKT010000180         2355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000180 DAZUKT010000000
VERSION     DAZUKT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.191"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..561
                     /locus_tag="ACG816_003056"
     CDS             <1..561
                     /locus_tag="ACG816_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4921854.1"
                     /translation="KHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRH
                     HKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFK
                     NKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVR
                     KVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            554..1981
                     /locus_tag="ACG816_003057"
     CDS             554..1981
                     /locus_tag="ACG816_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4921855.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1983..>2355
                     /locus_tag="ACG816_003058"
     CDS             1983..>2355
                     /locus_tag="ACG816_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921856.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGL"
BASE COUNT          818 a          279 c          377 g          881 t
ORIGIN      
        1 aaacatatag atgtatatca aaaacatgat tatcctagaa ttaataggaa taacacaact
       61 gcatttaaaa tttttagagc tataattaat cgttgtctga ggatgattaa acttccccca
      121 aaaattagac accataaatt tgatttgtat catggttctc aatggtgggc tttgtcgaaa
      181 gaatgcctaa cagaattaat acaaatgtat gaacaaaatc aacaagacta cctgaatttt
      241 aaaataggta tgtttgcccc agatgaaaaa ttttttcata cacttttttt taattcctcg
      301 ttcaaaaata aaaatgttat tggaggacca gatttaccac ttgaattgaa aaatattgaa
      361 gaaactactt tacaaacttc gaaattagca aatattcata ttattgatcc ttccatgaac
      421 aaatggtttg atgaaaggga tatagatcaa ataaaagaaa gtgaaaaatt atttgtaaga
      481 aaagttaaat cggattactc tgataaacta ttaaatagaa ttgataaaga aattttgaaa
      541 gttggaaata tagatgaata gaatagtacc tgttttagag aagagtttat taagtcgaga
      601 tgcgtttcga gtaataaatt taatcatttg tctgctttat tttatgccta gtacttttaa
      661 tgtaactaat atcccgatga aaatagcttt tgcttggggt ggaattatag ttttgtatga
      721 ggtttttata aagaaaaatt tttttaaaat gcgtttttct tggctactga ttggagcaat
      781 gattagtttt atcttaacta taatcattaa cagagaaaat tttttaattc catctattta
      841 taattttgga tatttgctga ttactttgat agtaattttt cctgcagact tttcacaaag
      901 tgaagaggaa aaaaagaaaa aaatggtgta ttttaataat gttttcatta tcataatttt
      961 tttagctgcg ctaatatcta tctatcaatt tatttttttg atttcttatc atgtcccaac
     1021 aggaacgcca ggattacttg caagacaagg ttttattgaa catcggttat ttggtgtcta
     1081 tacaagtcca aatgttggag ctatgtttgg atatacttct attattttaa gtttaattag
     1141 tgtattaatt tcaaaaataa caaaaacgat tcgtgttttt tatatgatga attttatcgt
     1201 acaaatattt tatattacac tttcttcgtc tagaggtgcc caggtagtaa tttgttcttt
     1261 tttagtatta tttcttttgt tgtttgccaa ttcaaaattt agaaagagca ttagagaagt
     1321 actacctgta aaaatcaaat ggatggtaag catattatta gttctattgg tatatttttc
     1381 ttttggtact aattatgcta aggatttttt agcaacaatt cctgtatcga ttgatagaaa
     1441 agtagaagtt atagagtcaa atgaatcaga taaaactcca cttccagaaa aagaagtaat
     1501 attacagcat tctagtgata actccgaaat ttcagctggt cgttttacga tttggaaagc
     1561 tgcgcttaaa gttataagac caactttgtt atttggttat ggagagaccg atttctatgg
     1621 gaatagcgat gcaaaatttt tgaaaaaagt agatcttgat actacagatg tgaatgaact
     1681 aaaaagagca catggaaaca tgcataatgg atttcttcaa gtacttgtat caagtggtat
     1741 aattgctttt atatgtatct atgctttcta tgtgttaaat atctttagtt tactcaaaac
     1801 attttttaga agtaaagtga attatgcgtt tcttggtatt gttttaattt ttatactatc
     1861 tatttttata gatgaaatgg tggaggctca tgtacttttc aataagcgcg atgtcatttc
     1921 aattgttttc tggtattatt tgggaagcat ttcttacttg tatttaaaaa aggagaatta
     1981 atatgaagac agctttcatt gcttggactc ctttacattt gattaatata ttaaatgttt
     2041 atcaaaattt ttaccgagaa tcaaaaggag atttatttat ttattctgaa tttgaaaacg
     2101 ctagaagata tagtgagcgt ataaaagaat tagggctatt taatgatgtt gtttttgtga
     2161 atcaccggaa aatcggttct tcctttaaac gtaaaattaa cttgttaact aaccataatc
     2221 aatttttctc ggatgattta tccgtttatg atgaaatttt tatacagggg gaaaattatt
     2281 tcacaaaaat acttttctcc aatgtttaca aaaaaaatcc agatgtaaaa ctaaattata
     2341 tagaagatgg tttgg
//
LOCUS       DAZUKT010000181         2349 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000181 DAZUKT010000000
VERSION     DAZUKT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.192"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..2200
                     /locus_tag="ACG816_003059"
     CDS             47..2200
                     /locus_tag="ACG816_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4921857.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          807 a          416 c          526 g          600 t
ORIGIN      
        1 tttgggtaca atgaagcatg aatgtaaata ggcggaagga taggaaatga tgaaagattt
       61 tatgaaaaaa atattaaata cttcttggtt agaaaaaata agaaaaggtg cacaagaacc
      121 taagaacaaa acgtttcgcc ggtcccatgt tccttttcgc ttaaattttc tttttttcat
      181 tatatttacg ttatttgttg cgttgattgc tagattgggt tatttgcaga tcgtgaacgg
      241 cgaagagatg gaggcgcgag tgaagtctac ttctacgatt acggtccaag aaagtactcc
      301 tcgcggaatg atctacgact ccacaggaaa agctttagtt acgaataaag cgaatcaggc
      361 catcacatat acgagaggaa cacaagttac ggctaaagat ttgcttgaaa tcgcaacgaa
      421 gctgaactcg ctgatcgatg ttccggtaga tgaaaatctg acagatagag ataaaaaaga
      481 tttttggctg gctgatccca aacatttgaa agaagccaca aaaagacttt cggcaaaaga
      541 aaagcaatta gggaacagcg aacaatatag tgcgatggtc aataaagtca cagctgatga
      601 gattaattta aacgaggagc aattaaaaat tgctaccatc tttaaacgga tgaatagtgc
      661 atatgaatta aacactgtct atatcaaaaa ctctggtgtt actgatcaag agttagctgt
      721 tgtggcagaa cgcgcttctg aacttccagg agtttcgact ggcacagact ggacacgaga
      781 atataatgca gcaagttcac tgaaaagtat tcttggcagt gtgacgactg aaaagcaagg
      841 acttccagca gatgaagcag agaaatacct agcaaaaggt tattctcgga atgaccgtgt
      901 aggacaaagt tatttggaaa aacaatatga agatgtcctg caaggaacca aaacccaata
      961 tgaagtttcc cttgataatg aaggaaatgt ttcgaatcaa aaagaaatct tctctgggga
     1021 aaaaggttct aatttaatgt tatctatgaa tgcagaattc caatcaaaag tagaagaaat
     1081 tttgaaaagg aattatcaaa cattgatcaa caacggcaaa gcacagtatt cgcctggtgc
     1141 ttatgcagtg gctatgaatc ctcagacagg agaagtgcta gcaatgactg gtttcagtca
     1201 tgaacaaggt tccaaagaaa tcaccgagaa tgctttaggc acgattacta gcgcctttga
     1261 tccagggtct gtagtcaagg ctggtacttt gacagcaggc tgggcaagta acgcgatcag
     1321 cggaaatcaa gttttgattg atgagccgat tcgtctgcaa ggtgcttcag aaaaaagctc
     1381 tgtctttaat cgaagcggac aagttgctct tgatgcagtc aaagcatcgg aattgtcttc
     1441 taatacgtat atgatcaaag tggcgttaaa gatgttagga ctagactata cacctggaat
     1501 gggactacct agtttagatg aagaagctaa agcttatcag caattgagag actctttcaa
     1561 agagtttggt cttggtacaa cgactgggat cgatttgccg aatgaatctc ctgggatctc
     1621 acgttctgtt gattatatga agaagttcaa tgcggataat ggcaaagaat ggtatgctcc
     1681 aggtaacttt accgatttgg catttggaca gtttgacaca tatacaccaa tccaattagc
     1741 tcaatatgct tcaacagtcg ctaatggcgg gaaacgagta cagccgcacc tagtcaaagc
     1801 gatctatggc aatgacgaaa atggaaactt gggtgaagtg aagaaagaaa ttggcacaac
     1861 agtagaaaat acggtcaata tttctgcaga aaatatggcg atcctccgtg aaggattcca
     1921 ccaagttgtc catggtaccg atccttacac aacagccaaa ccattagcca gtgcgaaaat
     1981 ggacctttcc gcaaaaacag ggactgcaga aacggttgcg gaaggtcatc cagatattgc
     2041 gacagtcaat agtaatatcg tggcatacgg accaacagac aatccggaaa ttgccatcag
     2101 tgttgtcttg ccgaacttat tagacgaaca agaccacata aacttgacga ttgctaaaga
     2161 aataatggac gcgtattatg atatgttcat ggcaaaataa aaaagaggtc gtgaaaaagc
     2221 cgcttagttt cgagtatgaa gaaaaatttt gtgaaaatag cttctcacat ttttgcttaa
     2281 atttgacttc ataccgagaa actggctttt gaacaccgtt tactcggcaa aaaaggttgt
     2341 gacaaaaat
//
LOCUS       DAZUKT010000182         2341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000182 DAZUKT010000000
VERSION     DAZUKT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.193"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            223..870
                     /locus_tag="ACG816_003060"
     CDS             223..870
                     /locus_tag="ACG816_003060"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4921858.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            1153..1226
                     /locus_tag="ACG816_003061"
     tRNA            1153..1226
                     /locus_tag="ACG816_003061"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1187..1189,aa:Ile,seq:cat)
     gene            1772..>2341
                     /locus_tag="ACG816_003062"
     CDS             1772..>2341
                     /locus_tag="ACG816_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4921859.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          832 a          332 c          428 g          749 t
ORIGIN      
        1 tgcaaatttg tccataactt attgattatc ttgtaaaaat gcggtaattt aatagttagt
       61 gagaagaggt gcttaattat agttaaaaaa gaggaaatag aagcagcatg taaccaagca
      121 aatgttaggt ttattggaga agctttatct ggatatccta agtataaata cttacacaac
      181 tggttacaaa gtaaaagttt ttatcttaat aaagaaagat gcatgcctaa taaaaattat
      241 agggtttata aaagaggaac tattgtatat gttgattttg gggtgaacgt gggttatgag
      301 ttatctggta atcattttgc tattgtttta aataataaag ataattcgaa aaatggtgtt
      361 gtatgtgtag taccaatctc ttcaaaaaag aaaagtaatt atgtatctgt aggaaaaata
      421 ttagaagttg ctagtattaa acaatttata agccaagctg ataaattaaa agagaaactt
      481 aggataatta ttttactttc attaaataga ggcatcattg atgaaagcga tttgaatagt
      541 tttattaaag atattgaagc taaaggtgaa aagctttcag tggaagaagt agaaaaacga
      601 gctgtaaaac ataatttggt ttgcgagaca agggaacaag taattaaaga aataaaatca
      661 ttatctgaag atatggtaaa atatcagaag gtgtttaatt gctacaagaa atatacacaa
      721 gaatcctatg taatgccact taatatccaa acgattagta aaaatagaat tagaagaatt
      781 aacaagtttg atccttctgg aaatatgaag gcacctggaa atgtcctcaa tgaagtagat
      841 gaaagtttaa aaaaatattt tacaaagtaa acgatatttt tgttgacatt tttctgttcg
      901 ttagttacca tgtagttata gaaggacgta agcgcctatt tttaatgtac acatcgggac
      961 gtatgcgccc acatagaaac atagttgaga gacatctata tggtgtctct ttttttatta
     1021 atatgcaaaa aagtctagtt ttttcaataa tatttatgta ataataaatt agccaccaca
     1081 acaaagaatg aaacccatta ttatctagtc tatgtccatt ctttttgttt gcagtagttg
     1141 tgatggcttt ccgtactctt agctcagttg gttagagcag acggctcata accgtccggt
     1201 cgtaggctcg agtcctacag ggtacatact ctaagtgatt taaattgcga ataaaatata
     1261 aaaatgactc cccttttgac tcccccaagc agtatatgga tgtctctaaa agttgttttt
     1321 ttagataata aaaagctgat aataagcgaa aaacacttaa aatcagcttt aaaccctatt
     1381 ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt accttcctta
     1441 tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga acggccaaac
     1501 aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat gcaagctttt
     1561 gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc cccttattgt
     1621 tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt ataaatgcta
     1681 gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta agataaaagt
     1741 gtaaaaacga tttgttttag gaggggaaac gatgtgtacc gcaatcactt atgcaacgaa
     1801 agaccattat tttggaagga attttgatta tgagatgtct tataatgaag tggtcactat
     1861 tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata aacattatgc
     1921 aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg ctacaaatga
     1981 aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata aagaattaca
     2041 agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag ggcaatgctc
     2101 aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata tcgattttag
     2161 tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa aatcgatagt
     2221 aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg tgttgacaaa
     2281 taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc tatcaaatgg
     2341 a
//
LOCUS       DAZUKT010000183         2316 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000183 DAZUKT010000000
VERSION     DAZUKT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.194"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..542
                     /locus_tag="ACG816_003063"
                     /pseudo
     CDS             288..542
                     /locus_tag="ACG816_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="ACG816_003064"
     CDS             612..1124
                     /locus_tag="ACG816_003064"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921860.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="ACG816_003065"
     CDS             1121..2158
                     /locus_tag="ACG816_003065"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4921861.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          590 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DAZUKT010000184         2293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000184 DAZUKT010000000
VERSION     DAZUKT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.195"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..>2293
                     /gene="esp"
                     /locus_tag="ACG816_003066"
     CDS             25..>2293
                     /gene="esp"
                     /locus_tag="ACG816_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS4921862.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT          827 a          350 c          471 g          645 t
ORIGIN      
        1 ctattaaggg agttgatttg aataatggtt agcaagaata ataagagagt atttctagaa
       61 aaaacaaaga aacgagtatt gaagtacagt atcaaaaaat taagtgttgg ggttgcatct
      121 gttttggttg gtgttggtct tgtcttggga acaactgaac tagttcaagc acaagatgaa
      181 atttctccaa gcaccccact tgaaacagcg ataagctccg tacaagtagg tgacaaagta
      241 gcttcgggga atacttttca ggagaatcct ggttatacga aaaattataa cttttcggat
      301 ttacaattta gtcctcaaga actaactgga gacacactaa aaggcaatac aattggattt
      361 gaagtttacg gaaaacataa tattgcagca tcaactaaaa actgggaaat ccgtcttcaa
      421 ttagatgaac gattagctaa atatgtagaa aaaattcagg tcgatccaaa gaaaggaatt
      481 ggatcttcta gacggacctt tgtaagaatt aatgattcgc tgggtagacc tacaaatatt
      541 tggaaggtca attatattcg tgcaagtgat ggattatttg ctggggctga aacaactgat
      601 acacaaactg ctcctaatgg ggtaattacg tttgaaaaga gcttagatga aatttttaaa
      661 gagataggca tagacaatct caagaccgat cgtttaatgt atcgaatcta cttggtaagt
      721 catcaagacg atgataaaat tgtaccaggt attgatagta cgggctactt tttaacagat
      781 tcagatgatt tctataatag cttagatgta tctgaaaata atcctgacca gtttaaacat
      841 ggatcagtaa gtgcaaaata tgaggaacct aatactcaaa caaaagatgg ttcaggatcc
      901 acaggtgcta atggagctat aatcttagat cataagttaa cgaaaaatta taatttctca
      961 tattcagctt ccgcaaaggg cacgccttgg tatgctaatt acaaaattga tgaacgatta
     1021 gttccatatg tagcgggtat acagatgcac atggttcagg ctgacaaagt aacatatgat
     1081 gtttcttttg aatcagggaa aaaagtggct gatttagcaa ttgaacggcg caaagatcat
     1141 gaaaattatg gtgtaggttc aattactgat aatgatctta ccaaacttat tgattttgct
     1201 aatgcaagtc cacgtccagt cgttattcga tatgttttac aattgactaa gccattagat
     1261 gaaatcttag aagatatgaa agcaacagca caagttgaag aaaataaacc atttggtgaa
     1321 gatttcatct ttgattcttg gttgtcggat acgaataaaa aattaatcca gaacacttat
     1381 ggaacaggtt attattattt gcaagatatt gatggtgatg gaaaccctga cgataaagaa
     1441 gagagcggag acacgaatcc atatatcggg aaacctgaat tagaagaagt atatgatgtt
     1501 gacacaacag ttaaggggaa agtattcatc cacgagttag cgggaacagg tcacaaagcc
     1561 caacttgttg ataaagaagg tactgtatta gcagaaaaaa ctatcgctcc aaatgaaaaa
     1621 gatggggctc caatttcaga tactgtagaa tttgaattta cgggtgtaga ttcaagtaaa
     1681 ttaatcgcga aagatgaatt aaaaatccaa atcgtttctc caggttttga taaaccagaa
     1741 gaaggttcaa ccgttattaa ggaatcacca aaagcggttg ataaacaaac cgtggtagtt
     1801 ggatttaaac cagatgctaa agaatcaatt cggaataata aaaacttacc tgaagatgca
     1861 gagtattcat ggaaaacaga gcctgatact tctaacgtta ctgatagtac gaaaggtatt
     1921 gtaactgtta agatcggaaa tcgaactttc gacgtggatg tagagtttgc tgtaaaagct
     1981 tctcaagcta tggaaaatga tgcaacatac gtacctataa caacaactcc agaaacgaca
     2041 gttcaaagtg gaaaacctac atttgataaa ccagatgttc ctttagctaa agatgccttt
     2101 tcaattttag atgtctataa taaggacttc ggtaatgcaa gtgttgatgc aaatactggt
     2161 attgttacat tcactccagc taaaggtgta ggagaatcgg agccgattac tggaacaatt
     2221 cctattaaaa ttgtttacca agatggttct gtaggcacga ccgatttagc agtaactgta
     2281 agtaaagata ttt
//
LOCUS       DAZUKT010000185         2125 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000185 DAZUKT010000000
VERSION     DAZUKT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.196"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACG816_003067"
     CDS             <1..159
                     /locus_tag="ACG816_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4921863.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACG816_003068"
     CDS             242..859
                     /locus_tag="ACG816_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921864.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="ACG816_003069"
     CDS             1023..1355
                     /locus_tag="ACG816_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921865.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="ACG816_003070"
     CDS             1679..1840
                     /locus_tag="ACG816_003070"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFS4921866.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="ACG816_003071"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="ACG816_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DAZUKT010000186         2108 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000186 DAZUKT010000000
VERSION     DAZUKT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.197"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            581..1540
                     /locus_tag="ACG816_003072"
     CDS             581..1540
                     /locus_tag="ACG816_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4921867.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..>2108)
                     /locus_tag="ACG816_003073"
     CDS             complement(1527..>2108)
                     /locus_tag="ACG816_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921868.1"
                     /translation="IKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSNKKNIIIET
                     YAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIGLIYKIIED
                     DKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSLDFEIPMK
                     QMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          727 a          343 c          310 g          728 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaat
//
LOCUS       DAZUKT010000187         2104 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000187 DAZUKT010000000
VERSION     DAZUKT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.198"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACG816_003074"
     CDS             complement(387..1853)
                     /locus_tag="ACG816_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4921869.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          662 a          419 c          342 g          681 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttac
//
LOCUS       DAZUKT010000188         2056 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000188 DAZUKT010000000
VERSION     DAZUKT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.199"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            493..714
                     /locus_tag="ACG816_003075"
     CDS             493..714
                     /locus_tag="ACG816_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921870.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="ACG816_003076"
     CDS             715..972
                     /locus_tag="ACG816_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921871.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="ACG816_003077"
     CDS             complement(1108..1710)
                     /locus_tag="ACG816_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4921872.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="ACG816_003078"
     CDS             complement(1777..1932)
                     /locus_tag="ACG816_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFS4921873.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAZUKT010000189         2004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000189 DAZUKT010000000
VERSION     DAZUKT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.201"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG816_003079"
     CDS             <1..378
                     /locus_tag="ACG816_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4921874.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG816_003080"
     CDS             426..1826
                     /locus_tag="ACG816_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4921875.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DAZUKT010000190         1954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000190 DAZUKT010000000
VERSION     DAZUKT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.202"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..572)
                     /locus_tag="ACG816_003081"
     CDS             complement(174..572)
                     /locus_tag="ACG816_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4921876.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(569..1342)
                     /locus_tag="ACG816_003082"
     CDS             complement(569..1342)
                     /locus_tag="ACG816_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4921877.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1339..>1954)
                     /locus_tag="ACG816_003083"
     CDS             complement(1339..>1954)
                     /locus_tag="ACG816_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4921878.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
BASE COUNT          617 a          480 c          300 g          557 t
ORIGIN      
        1 ttttttcatc atttttaaaa tagcatattt tgaaaagaaa gtctgatgct tagagtagaa
       61 tttcataaaa atttactatt tcataagtat tcacgagaaa aatacaaaca aaaaagcgga
      121 aataacgctc atccgccttc ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat
      181 tcagactttt ctctatcgtt attttcatct aaaagttctt gcattccgat aatttctcga
      241 tattgatgaa tcttagtgat accaatgact ttgtctacat caatactaaa acaaatacca
      301 ttacctggaa tatcgatgtt gatgccttga ccaatcgcat ccattacttt attcgtcaaa
      361 ctatcgggta ctaatgtcaa cacaatatct ttttctggcg aaatgtccaa acctaaaaat
      421 ttccctgaat catgtacacc agaacctctt ccatgtaaga tcgttccgcc tgctgcacct
      481 gcttcttttg aaaagtcgat aacatcttcg cccattcctc cgtcaacgat cgtcacaatc
      541 atgtgaagat tcaatgctac aagatctttc atttgctttc ccccaatttt tgcaatcttt
      601 ctttttcgat tctgtctttt tcatcttttc gtttataaat atatcctagt actaaagtgg
      661 ataatattgg tgtcaatgca atcattgcaa ccacaccaaa cccgtcaatg atcggatctc
      721 ttccttcaat ttgtgcagcg attgccacgc tcattgataa aataaatgtc gaacacatcg
      781 ggcccgttgc tactccaccg ttatcaaaag ccattgctaa aaagatcttg tccacttttc
      841 gtcctaaaat aaatgctagc ccgtatcctg ggatcaagaa ataataaaga gagaatccga
      901 cgagcaaacg ccacatagac aatcctactg ctgcgccgat cccaattgaa attaccatca
      961 gcatgatttt cgatcggatt ctcccctcgc tcaattcttc aacctgtttg atcattacat
     1021 gaatcgccgg ctcagcaaac cctactaaga accccattaa aaagccaagg ggaaccagta
     1081 tataattgta aggcaatccc gcaatagcac tgcctaaatg ctgtcctacc ggtacataag
     1141 ccatattgac tccgtgcagg aaaaggatca acccaatagt cgttatcaag aaacctttca
     1201 taatgtcaaa gaaacgcttt ttgcgcatac gaaaactaat gatattcatt aaaacaaata
     1261 ggattacgat aggtgccact gccaaaatga cttccatggt aacgatttcg attccatcta
     1321 tcacttgctg ccagaaactc attggaagat cacccccaaa atcatcacgg caagaatcgg
     1381 ccctaacgaa gcaataccga ccattccaaa actgtcattt tcatttttct tttgtttgat
     1441 catactagtc atccctccgg caagggccaa gataaatgga acagttactg gaccagtagt
     1501 cactcccccc gaatcaaaag caatcggcat aaattcggaa ctggtgaaaa atgaggcgat
     1561 caaaacgcct atgtagccta tagccatcaa ttgataatag gatagcttga atacgacccg
     1621 aagcagcgca aaggctagaa atactccagt gccgacgctg acgatcccaa tcagcaacac
     1681 tctgccaatc tctccgtcag atatatcata tacttgttgt cccaaaactt gcacggaagg
     1741 ttcagcaatc gtgatcacga tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact
     1801 tttctttttg atcatatatt tccctactag atctccgact tccatcatag aataatctgc
     1861 tccaaacaaa aagagcgtca ttccgatcat catgattgcc gcaccaacca gaaatgaagc
     1921 gaggtcttct ccttctaacg gtgcaaaaac aaac
//
LOCUS       DAZUKT010000191         1899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000191 DAZUKT010000000
VERSION     DAZUKT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.203"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1073
                     /locus_tag="ACG816_003084"
     CDS             <1..1073
                     /locus_tag="ACG816_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4921879.1"
                     /translation="KKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANN
                     IDKDFFKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFH
                     EPLIEYTGNLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFN
                     LSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVS
                     LPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVS
                     ETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFK
                     KYAGVSPKQYQLGLVDNFNNIS"
     gene            1170..1523
                     /locus_tag="ACG816_003085"
     CDS             1170..1523
                     /locus_tag="ACG816_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921880.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
BASE COUNT          710 a          219 c          316 g          654 t
ORIGIN      
        1 ataaaaagtt actaactgat aatattaaaa aaactaaaga tttccatttc tttactggtg
       61 attttaatga gatgtttctt ctatacatgt ttaaaaccag atattactta tttggtccat
      121 ttagagcaaa taatattgat aaagattttt ttaaactaaa aatgaacaat ttgaatgttg
      181 caatggctga tagagaacga ctctataatt cattgcaaaa tttgactttg tattctttag
      241 gagacattcg agatattctg attttagttc attatttttt tactggaaaa atagaagatc
      301 tattccatga accgttaatt gaatatacag gaaatctaag taaaactatc gagcaaatca
      361 aaatagataa tctgttatca caaaattacg atccagaaat atatctgttt ctttatgaaa
      421 ataaaatact ggaatatgtt aaaaatggag atattcgaaa tttagaaaac atggttttta
      481 atctgagtaa tggaataatt ccaagtgtta gtggagatac gattcggtct gaaaaaaatt
      541 attctatcat tgtttttgaa aagttagcac aaacgtctat aacattagga atggatataa
      601 tagaagcgta tcaaagtaga gatgctttga tacaagaaaa cgaattagct gtatcgttac
      661 ctgaggtctt aaaagtgagg gattctggga ttgtttatta tactaaggaa atcggaaaaa
      721 ctaagataga gcatctttct cccttaattt cttctgtcgt tcaattcatc ggcttaaata
      781 tctataaaag aattactgta aaggaaatag ctaattattt ttctgtaagt gaaactaaat
      841 taagagaaag ctttaagaat gaaatgcata ttacgattta taattatatc tctaagcgaa
      901 aaatttctac ggctaaaata atgttgaaat caaaccatac tatcagtgaa gtttctttag
      961 gtttaggatt ttcagattct tcacactttt cgagagtatt caaaaagtac gcaggtgtat
     1021 ctcctaagca atatcaatta ggtttagtgg ataatttcaa caacattagc tgagaggttt
     1081 tattttatta tgagactata tcatttacgt agtttggagg agttgaggaa attggatttt
     1141 aacaaaaaat tttttgggaa atttctgatt tgagttgttt cgtagaatct gagcgtctaa
     1201 gtaatggtaa taatagtgtg ttaaatgaat atgacaaagt agtaattaat aaagtaaaaa
     1261 gtttatgtga agaagctaga gaatctatca gtcatattaa tggtagcaat tttaagcaaa
     1321 cattatttaa tctgattaag ctagatgcta aaatatcaag ttatttattc tttttaatac
     1381 atgatgaatt tatggattat caaaaattgg atcagttaat tgaaagagaa agctgggaag
     1441 attattacgt gggtctaact ttttcagaaa agttaaacaa ctacaagtta atagattata
     1501 agtatttgag tgaaagcaat taattattca tgtagagtac tagacacttt aaatatggac
     1561 aattcaatta taaagaaaaa gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt
     1621 tgtttcgcta aaaacgagtg aaaaaatgtt gataactcca atcattcgtt taagattcta
     1681 ggaatcattc aggaatgctg tgaaaccgat actttataat tataaagaag gtgatttcaa
     1741 attccgaaga aggaatttga agtcaccttt ttacttatgt cacagtctca ttcagtaatg
     1801 cgggataagg ctccttattc cgctatttga atactctgaa gtttaatcta agaaagagta
     1861 atattttgaa aacatgcttc atcgcataga aatgaactt
//
LOCUS       DAZUKT010000192         1872 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000192 DAZUKT010000000
VERSION     DAZUKT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.204"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..634
                     /locus_tag="ACG816_003086"
     CDS             <1..634
                     /locus_tag="ACG816_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921881.1"
                     /translation="NKNSIFKKKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNI
                     QSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRART
                     NRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLIL
                     NEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            621..>1872
                     /locus_tag="ACG816_003087"
     CDS             621..>1872
                     /locus_tag="ACG816_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4921882.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFD"
BASE COUNT          630 a          240 c          290 g          712 t
ORIGIN      
        1 aaataaaaat agcatcttca aaaaaaaaat tgacgattat tttgtctatt gtccggaatt
       61 gttactttat gatgtaaaaa atataaaaaa aattcccaaa atagatgaaa acagtaaatt
      121 gatgaaaaac atacaatcaa tctttgggat agaagaagtt tttgtcggag ataaatgtat
      181 cttttttgat caacctttat tatctgatgg ttatggcata gatgaaacag atattttcgc
      241 taaagtaaat agtatatttt ctggaaaaaa agaaacttta attaagcttc atcctagagc
      301 aaggacaaat cgttatggtg aaaaaaacat attgaattcc agtttaccgt ttgaagtctt
      361 gctacttgaa aatgacttct cttcaacact tttgattagt cctatatcta caatttcttt
      421 ttcaccatct atgatgtttg aaaagacgat caactctatt atgttggcta aattaatttt
      481 aaatgaatat acagcattat ctgaagataa aaaagaaaca ttgacaaata ttgttctttt
      541 ctgtgaacat tataaccgtc tatcagaaga aaaaatttta ctcccagaaa actgggcaga
      601 attaagaaag ttggtagagt atgggattat ttaacacact ttccaagtca aaattagtta
      661 agtccagttt ctggtatact atagggaatt tttttattaa gggaatttca ttcataacta
      721 ttcctatttt tgtaaatatt actactcaag cagaatatgg tttgattaat aatttcactt
      781 catttgtatc aattttttcg attcttgttg ggctaagcct aaattcggca attaataatg
      841 caagttttga ctataaagat gacataaatg aatttatgtc atcggtactt tctctagcta
      901 cactttcgtt aattgtgttc ttattaatag ggaacatata ttttttgttt tcaaatgctt
      961 tttttgatat gaaccaactt gtatttaatt tgatgttatt acaaagtttt gctagttttg
     1021 taattagtta tatccagtct tattttacga ttaactttaa ccattttaaa tatttgattt
     1081 tatcttttat atctactttt ttgaatattg caatctctgt agctttgatg cagacattat
     1141 attttgatga taaatatgtt ggcagagtag tcggcggaac actgtcattt gtgttgatag
     1201 gtttagtaat ttatttaaga ataatgataa agggtgctca tttttttgat aaaaaacagt
     1261 ggagatacgc tttaaaaatc tctttgccgc taatcccaca ttccttagca aatatagttt
     1321 tagcacaatt cgatagaatt atgattaatt cttatttagg tcctgctgcg gggggaatat
     1381 attcctacat tagtaacata ggtattattc taagcgtaat atggatgtct actaataacg
     1441 cctgggttcc atggttctat ggtgagatga gtaaaaataa tgaaaaaaaa gtgaagaaag
     1501 ttagtaacta ttatttagta gtctttactt tgattacgat gattgtaatg gtagtttcga
     1561 ttgatttagc aaaatttatg gctccgaaag actattattc tgggttatct ttagtggttc
     1621 cgataacctt aggctatttc tttcagtttt tatatagttt accagttaat gctgaatttt
     1681 atgagaaaaa aacatcgttt attgcgattg gtacagtagc aagtgctata gttaatatac
     1741 tattaaacat gattttcata cctaggttag gaataattgc tgctggatac acaaccgtat
     1801 ttacgtattt tttacttttt atttttcatt ataatatcgc taaaaaaatt acgaataaac
     1861 aattatttga ca
//
LOCUS       DAZUKT010000193         1680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000193 DAZUKT010000000
VERSION     DAZUKT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.206"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG816_003088"
     CDS             64..972
                     /locus_tag="ACG816_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS4921883.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACG816_003089"
     CDS             972..1592
                     /locus_tag="ACG816_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921884.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          602 a          246 c          377 g          455 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
//
LOCUS       DAZUKT010000194         1677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000194 DAZUKT010000000
VERSION     DAZUKT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.207"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG816_003090"
     CDS             64..972
                     /locus_tag="ACG816_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS4921885.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="ACG816_003091"
     CDS             972..1589
                     /locus_tag="ACG816_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921886.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          620 a          226 c          348 g          483 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaa
//
LOCUS       DAZUKT010000195         1664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000195 DAZUKT010000000
VERSION     DAZUKT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.208"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG816_003092"
     CDS             131..505
                     /locus_tag="ACG816_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4921887.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG816_003093"
     CDS             complement(619..1248)
                     /locus_tag="ACG816_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4921888.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG816_003094"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG816_003094"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZUKT010000196         1653 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000196 DAZUKT010000000
VERSION     DAZUKT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.209"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1143
                     /locus_tag="ACG816_003095"
     CDS             <1..1143
                     /locus_tag="ACG816_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4921889.1"
                     /translation="NHEVKTIDADKKTVTVTSASDNETKEYPYDKLILSSGVKPNNLP
                     VPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVGAGYIGIEAAEASRKAGKEVVL
                     LDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKIEAFTGNGKVEAIKTSEKEIPA
                     DLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTNLPDVYAVGDATLAYSIPARKK
                     MPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSALSVFDYHFAASGLNSFTAAKA
                     GVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMTHQLLGGAVLSTYDVTGQGNVL
                     ALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQALGEEPFVE"
     gene            1424..1653
                     /locus_tag="ACG816_003096"
                     /pseudo
     CDS             1424..1653
                     /locus_tag="ACG816_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a          293 c          356 g          413 t
ORIGIN      
        1 aatcacgaag taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca
       61 gataacgaaa caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca
      121 aacaatctgc cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat
      181 tgggcaacca aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta
      241 ggtgcgggtt acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc
      301 gtattgcttg atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat
      361 attttagaac aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa
      421 gcttttactg gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca
      481 gatctagtca ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc
      541 gacttagatg aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat
      601 gtgtatgcag taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaatgccg
      661 atcgctttag cgactgttgc acgtagagaa gcacgttatg tagtaaaaca tctttttgaa
      721 gaagtaccaa acaaaccgtt cggcggagtc gtaggttcct ctgcattaag tgtctttgac
      781 tatcattttg cagcaagcgg attgaatagc tttacagcag caaaagcagg agtagatgta
      841 cgagtgtctt attatgaaga tacgttacga ccaaaatatg taccagaaga acaaggaaat
      901 ccaaaagtgt ccatccaatt attctcggat ccaatgaccc atcaattgct aggaggagca
      961 gtattatcta cctatgatgt tacaggtcaa ggaaatgtat tggcactagc gatccaaaac
     1021 aaactgactt tagaagaact agcagaagct gacttcttct tccagccagg ctttgaccga
     1081 caatggagca tgttgaatct agccgctcaa caagcgttag gagaagaacc atttgtagaa
     1141 taagaaaacg aaatatttga aaagaggttg tgaaaaagct gccttgcatc aagtaataag
     1201 aacagccaag taaaaacagc ttcttatgtt tttaacttgg ctggacttat taccgcagat
     1261 gcaagctttt gaacaccgtt tattttcaaa aaagggactg tgacacgatt tgtgtaccga
     1321 cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggttaaat
     1381 atacttttga acttaaatta aagatcgttc atgattattt ggatggcaaa ggcgattgga
     1441 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaaattcggg agaagcacaa
     1501 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
     1561 aaagaaagtt cagcgcttga tcaagaagct aggaataaaa gtaacggcat acacgcgtaa
     1621 atcacgtcgc tacaattctt atcgaggaaa agt
//
LOCUS       DAZUKT010000197         1573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000197 DAZUKT010000000
VERSION     DAZUKT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.210"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAZUKT010000198         1526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000198 DAZUKT010000000
VERSION     DAZUKT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.211"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..734)
                     /locus_tag="ACG816_003097"
     CDS             complement(<1..734)
                     /locus_tag="ACG816_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4921890.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
     gene            complement(731..1150)
                     /locus_tag="ACG816_003098"
     CDS             complement(731..1150)
                     /locus_tag="ACG816_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFS4921891.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="ACG816_003099"
     CDS             complement(1220..1360)
                     /locus_tag="ACG816_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4921892.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
BASE COUNT          417 a          327 c          236 g          546 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtgg
       61 gcgtaatttg ttccatctgt cagtgtagga tttgctgcac ctgctctagt tggatcaatg
      121 actggtttat catctgaagt tgtcggattt tcatcggtaa atccatgagc taaatcatat
      181 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      241 ccccagatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggcgtt
      301 tctaatgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgtgtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacttca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagaat gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaaaaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccact
//
LOCUS       DAZUKT010000199         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000199 DAZUKT010000000
VERSION     DAZUKT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.212"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG816_003100"
     CDS             complement(150..1337)
                     /locus_tag="ACG816_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4921893.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZUKT010000200         1418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000200 DAZUKT010000000
VERSION     DAZUKT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.213"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..443
                     /locus_tag="ACG816_003101"
     CDS             33..443
                     /locus_tag="ACG816_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4921894.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            457..813
                     /locus_tag="ACG816_003102"
     CDS             457..813
                     /locus_tag="ACG816_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4921895.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          522 a          208 c          266 g          422 t
ORIGIN      
        1 gaattggatt ataattaaaa aggagtgaga aaatgataaa agtatatact tcgcctagct
       61 gtacttcctg tagaaaagca agagcatggt tgcaagcgaa tcaacttgaa tttgaagaga
      121 agaatatttt tgccgaaccg ttaacagaaa gtgagatcaa aaagatcttg gcagcgactg
      181 aaggaggagt tcaagatatt atttccactc ggtcgaaaat ttacgaaaag ctgggcatag
      241 attttaacga attaacttta aagcaaatga tcgtcttatt caaagaatat ccttcattat
      301 tgagacgacc tattttgatt gatgaaaaaa gaatactaat cggatttaat gaagatgaga
      361 tccggtcatt tgtccctcgt gaaattcgat gtgttgccag aaatctgatc tatttacaaa
      421 acaataacat cttaaattca tagggaggga gccttcgtgg aagtgaaagt ctatggatca
      481 gcgcactgtg taacaagtct gaaggcgaaa caatggttag aacaccatca attgccttat
      541 caatttgttg acttagaaga acgcgattta agtacagaag aagcagaaga attatgcagt
      601 tttgaagaag tgcaagcaga acagctattt gcgacttggt ctgaggcttt tcacaacatt
      661 gcgattgacc tttcctgtgt aggaaaaaaa caactgattt ttttatgcac acaacatacc
      721 aatctcctca gacgtccttt gattattatc gatgaagttc tatttattgg atacaatgag
      781 cagttgatgg aacagcttat cttaaataaa tagactttaa ctgttttaat caagtcactg
      841 agcatttcat acagttgtaa cagtcgaaca cttagcatca agcgtgttat gtgttctttt
      901 tttgacttta tttttctact atagagacat gctcaattta tgtggtcaaa attcaaatct
      961 aggtggggat atagagttaa aaatatattg acatgtgtaa atttacatga tatattcatt
     1021 tctgttgttt taagtagcaa agcaataaaa tagtatttta aaaaacatat aaaatagttg
     1081 ttgacatgtt agaaatgaca tgataagata actgagttgt taaaaaagaa ccacttgtga
     1141 agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa atgataagtt
     1201 atgataaatg agttgctgta agatttcgaa aaacgaaatc aaaaaaactt ttaaaaaaga
     1261 gttgacaatt acttgtctct ctgatatgat ataaaagttg ctacggcagc caaaaacgaa
     1321 tcggggaaca ctcctgatga agtagacctt tgaaaactga acaaagtaag acaaaccaaa
     1381 tgtgtagggc gtcttgattc aattcaagac aacaaaca
//
LOCUS       DAZUKT010000201         1368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000201 DAZUKT010000000
VERSION     DAZUKT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.214"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1368
                     /locus_tag="ACG816_003103"
     CDS             82..>1368
                     /locus_tag="ACG816_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4921896.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAZUKT010000202         1367 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000202 DAZUKT010000000
VERSION     DAZUKT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.215"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1351
                     /locus_tag="ACG816_003104"
     CDS             56..1351
                     /locus_tag="ACG816_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4921897.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          432 a          272 c          262 g          401 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat
      721 tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca
      781 gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg
      841 agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa
      901 aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa
      961 acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact
     1021 gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc
     1081 ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca ttagcggtct
     1141 gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa
     1201 tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt
     1261 attaaaacgc aatgcgtacg gctttcgcaa cttttacaat ttcaaactac gaattatgat
     1321 tcgtcatggc aaaacatttt taaccaaata aacagaacag aaagagg
//
LOCUS       DAZUKT010000203         1353 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000203 DAZUKT010000000
VERSION     DAZUKT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.216"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1006
                     /gene="scrK"
                     /locus_tag="ACG816_003105"
     CDS             134..1006
                     /gene="scrK"
                     /locus_tag="ACG816_003105"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4921898.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          427 a          235 c          311 g          380 t
ORIGIN      
        1 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
       61 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
      121 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
      181 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
      241 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
      301 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
      361 tacctcgacc ccgaaattgg catggcaaaa ttttgatttc atcggtacca tgaaaaaaga
      421 atttccgatc ccgatttctt ggacaacaga tgtcaatgcg gctgcctatg gtgagtatgt
      481 cttcggcagt ggcaaaggat tgagcagtgt cgtttactat acgatcggta cgggtgttgg
      541 cggcggtgcc ttacaagaag ggcgctttat cgaagggttt agtcatccag aaatggggca
      601 tatgctcgtc gtccctcatc caaaagatga ctttgctggc agctgtccat ttcatggcaa
      661 ctgtttagaa gggatggccg ctggacccgc catcgaaaac cgcttaggga aaaaaggaca
      721 agaggttgca gaagatgatc cttattggga aatcgaagcc tcctatattg ctcaatgtgc
      781 ctacaataca accttgatgt tttctccaga tgtgatcatt tttggcggtg gtgtgatgaa
      841 acaacgccat atgctgcaaa acgtccacga cgcatttgca aaattggtca atgggtatgt
      901 cgaaacacct gagttaagca aatatatcgt gaccccagca ttagaagaca acgctggaac
      961 gctcggttgt ttggcattgg ctcgagaagc ggtcttacat tcataaacga aaaaaggctc
     1021 ttcgacattt agtgttggta gagaaagata caactgaaaa tttagtcata accagttagg
     1081 cagagtgctt aactggtttt tgttttactt ggaagatcaa gccttgaatg agataaattc
     1141 ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac ttgtattgtg caatagtttt
     1201 ttacaaaaaa aagggggtgc ggatattttt ttggaccttt ttaacaaata ggttctgttg
     1261 caaagtttta aatctactat cagataaggt agaataatag aaaaagatag caggaggaat
     1321 gacgatgagt cattttaaag gaaagcaatt tca
//
LOCUS       DAZUKT010000204         1348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000204 DAZUKT010000000
VERSION     DAZUKT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.217"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..396
                     /locus_tag="ACG816_003106"
     CDS             <1..396
                     /locus_tag="ACG816_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4921899.1"
                     /translation="KDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYL
                     FPSTKGGHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFG
                     HSSEKITKRYIGINEDEISETLLNFRLGF"
     gene            709..1251
                     /locus_tag="ACG816_003107"
     CDS             709..1251
                     /locus_tag="ACG816_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFS4921900.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT          454 a          251 c          270 g          373 t
ORIGIN      
        1 aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg gaaaacacgc
       61 attttatact taagcagctt acaggacttg attcacggct ataccgcacc cttagatccg
      121 gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac ggtctaccag
      181 atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca caccctacga
      241 aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt aatggaaatc
      301 tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga agatgaaatc
      361 agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat agataagcac
      421 agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt ggtatattat
      481 atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca taagggtccg
      541 gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg gtccggcggg
      601 tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc tttttttatt
      661 gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat gtctataaaa
      721 gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca tctcttacaa
      781 gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt agcgattatt
      841 ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg cacacacttg
      901 ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat caaaacggat
      961 gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt ggggaagcat
     1021 atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc tattcgaaca
     1081 aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat ccctatctct
     1141 ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg catttttagc
     1201 gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta atcattaata
     1261 gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa taaagcattt
     1321 taagacgatt tcaaatcatt catgtggg
//
LOCUS       DAZUKT010000205         1337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000205 DAZUKT010000000
VERSION     DAZUKT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.218"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1337
                     /locus_tag="ACG816_003108"
     rRNA            <1..1337
                     /locus_tag="ACG816_003108"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAZUKT010000206         1309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000206 DAZUKT010000000
VERSION     DAZUKT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.219"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..>1309
                     /locus_tag="ACG816_003109"
     CDS             127..>1309
                     /locus_tag="ACG816_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4921901.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          411 a          261 c          235 g          402 t
ORIGIN      
        1 tctataattt ctaggactga tggaaatttt ctatctgtcc tagttttttt gtaaaaaaac
       61 aaaaagacat acaaaatttc cggtagaata gagtctccta caacatcatt ctgggggaat
      121 tcttgtatgt ccatgaatca ttttatcaaa caactcttta ctattaaaga ctcaaatatc
      181 caaattcaat cctttgaaga aactttttat aaaagcagaa aaagtatcgt tattgaggga
      241 actctgatcc gtacctacag acgttgtccg tgttgtaaag attctacaaa gcaaatcgtt
      301 aaaaatggca agaagatctc tatgattctc ctgaatcgta gcggtaataa acggacctat
      361 cttcggttga agaaacagcg atacctctgc cgagcatgta aaaaatattt taccgctcgg
      421 acgtatctcg ttaccccatt ctgctttatt tctaagcaga ttcattacaa gatcttagaa
      481 gaattgacag agcgtcaatc tataaaagca attggtaaac attgcgatgt ttctgtgaca
      541 actgttcagc ggacattgtc cgccttgtca tcagatatcc gtcagaatct tgagtattta
      601 cctcggtgtc tattattcga tgagtttcgt tctctttcta catggcacgg taaattttct
      661 ttttcttgta tggacggtga gaccgggaaa ctgtttgaga tcttacctag ccgccgtaaa
      721 aaagatctcg ttgctttttt catgcgtttt tctctcaaag cgaggttggg tgtacattac
      781 atcgtaacgg atatgaacgc tccttatttc tcactagtta aagagtgttt tccaaatgca
      841 caagtgatcg tcgatcggtt tcacattgtt caacatctga accgcttctt cgatgcggtt
      901 cgaaaacgtg tgatgaaaac acttgatcaa aaagacccaa tacaagctaa acagtatcgc
      961 caattgaaat ccttattcaa attactttta aaatcagagg atcgtttaga ttacaataca
     1021 ctaacgaaaa gacgcaattt caattggaga ctattgactg aaacagaagt agttgaccgc
     1081 cttctaaaga tttctcctga acttaaaaca gcttatcgtt actaccaaga cctgcttact
     1141 gcgtatcagg aaaaagatcc agatactttt ttccagctgc ttcgagcaat gcctggagaa
     1201 gttccattgg aactgcacca tatcaaaaaa gcctttttca aatacgagaa agggattcgg
     1261 ctggcattac tgaagaagta ttcgaatgca cgactggaaa atctccata
//
LOCUS       DAZUKT010000207         1307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000207 DAZUKT010000000
VERSION     DAZUKT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.220"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(358..431)
                     /locus_tag="ACG816_003110"
     tRNA            complement(358..431)
                     /locus_tag="ACG816_003110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Ile,seq:cat)
     gene            complement(986..1300)
                     /locus_tag="ACG816_003111"
     CDS             complement(986..1300)
                     /locus_tag="ACG816_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921902.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          482 a          205 c          195 g          425 t
ORIGIN      
        1 actccaatta taagtctttt tcccattatt ttttacagtt atatggtatg ctttttaatg
       61 gcttcaaata taaaagagtt taaagcgtaa cacacttatg gggaagtggt ttttggggaa
      121 cgctttaaac tcttctttat tattatctca caatataacc caaatgtctt tccatttaaa
      181 aatcaaagta aaacttttca aatatacaga agtataacta tgtgaaacat cctttcatta
      241 atccataaaa ggatacataa aaaagccact catttgagtg gcaatgaaga aaagctttag
      301 cttgtataat actcttcaaa aaattctaac acagaacgat tcaattggct acattaatgt
      361 accctgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct gaccaactga
      421 gctaagggta gtggttgttg ccacataaag ccataaacaa tcaaccagta gaatgcgtgg
      481 caacaaacct gttatcgcat atcttggagt gtgactattt atgagtgata gtgaagatat
      541 gcgtctccat atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca
      601 acgttttttt attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga
      661 ttttgcattc aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc
      721 tattttaagg tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat
      781 gctgatggaa ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttaa
      841 ttataaactc tagagaaatt aattcatcct aacataaaat aattgttcac tcaaaagttt
      901 ttctagaaca attaaaacag caggcttcac attcgctata catttgtaag aagtaataat
      961 tcctgtacta aaaggcataa ttgctttaat tattttttga cttctcttca aacacaagaa
     1021 gtagttgtgt aataattgca gaaataagga ttccaataat agatataaaa tttgacatgg
     1081 aaacgtctat aacattaaaa gaataaagtg ttgaagaaag taaactgatt gaaataaccc
     1141 ctccgtaaaa aatagtctgt gaatataccc actttttttc agaagacgta cttcgtttgg
     1201 ttctaaaacc tatccctgca tttctactct tatcaaaaaa ccaaatattt agaagtaata
     1261 atattgctat tattaagata aaattactgt aaaaatccaa aagctat
//
LOCUS       DAZUKT010000208         1294 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000208 DAZUKT010000000
VERSION     DAZUKT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.221"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG816_003112"
     CDS             <1..1244
                     /locus_tag="ACG816_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4921903.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZUKT010000209         1248 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000209 DAZUKT010000000
VERSION     DAZUKT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.222"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG816_003113"
     CDS             200..742
                     /locus_tag="ACG816_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921904.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1248
                     /locus_tag="ACG816_003114"
     CDS             898..>1248
                     /locus_tag="ACG816_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS4921905.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPD"
BASE COUNT          515 a          141 c          238 g          354 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagat
//
LOCUS       DAZUKT010000210         1242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000210 DAZUKT010000000
VERSION     DAZUKT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.223"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..359
                     /locus_tag="ACG816_003115"
     CDS             99..359
                     /locus_tag="ACG816_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921906.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(721..960)
                     /locus_tag="ACG816_003116"
     CDS             complement(721..960)
                     /locus_tag="ACG816_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921907.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          440 a          169 c          224 g          409 t
ORIGIN      
        1 atttttttat aaaatcacaa gtgttattga ttttcataat catattttaa tggtattatc
       61 attttaaata atggaaaaac attaaggatg gatgaaatat gaataacgaa actatcattt
      121 taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtggtt ggagaagtaa
      181 tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac gtcgatcgca
      241 atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt tatgagttgg
      301 aaacaacaga atatctatat aactatagca acaaatatga acatacttta gtttcataaa
      361 gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca ctttatttag
      421 ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga gaatctgtct
      481 ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa ctgtcctatt
      541 ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt tcaaaaaatg
      601 caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt tgtaaaggtt
      661 gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa gatgaagaag
      721 ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta ccctgttttt
      781 tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct catttgtaga
      841 aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct ttgatgcaga
      901 agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa ttcttttcat
      961 tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat taggcaaaat
     1021 caatgcaaaa ctatcaagtc ttttatagta tctattagtc ttagtaatac atatatggag
     1081 agggtgatat cccagttcaa accagataca ttggttacca tctgtattaa cgattatgcc
     1141 tctaaacgct ttttttatga aaaaatttat aagtgaaaac gtaggtatga tattatctag
     1201 aggtaatttt tttgggtatt taaatagttg ctaggaggat ag
//
LOCUS       DAZUKT010000211         1228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000211 DAZUKT010000000
VERSION     DAZUKT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.224"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1187
                     /locus_tag="ACG816_003117"
     CDS             <1..1187
                     /locus_tag="ACG816_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4921908.1"
                     /translation="HYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGK
                     TFLAEDSVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKT
                     VSLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYP
                     RKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFK
                     KGDRRSKHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGY
                     EVYQVFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNG
                     PIECLNNHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKI
                     AV"
BASE COUNT          384 a          276 c          246 g          322 t
ORIGIN      
        1 gccactatca aaataagcaa acgatcatta aatggggctg gaaaaaagta tccatcttat
       61 tgaatgatgt cagtaactac aagaccattg tgcggattaa caaacagcgc ttcaaatgta
      121 aacactgtgg aaagactttt ttagcagaag attcagttag cgatcgtcgc tgctccattg
      181 cccgcagagt caaacaagcc attcttgagc tattaagtga gcctatttct atgtctttga
      241 ttgcacgtat gaagcacatt tctccgacca ctgtcattcg aatacttcgc agccttcggc
      301 ctaaaaccgt ttctttgaac cagccgttac cagaagtggt gtgtttcgat gaattcaaat
      361 cggtgaaaaa cgtctcggga gccatgagct ttgtcatgat ggacggcaag acccatcaac
      421 tgattgatat tgtggaaaat cggcaactca atcctttacg cgactatttc ttgcgctacc
      481 ctagaaaagt ccgtgaacaa gtacgtctag tggtttcaga tttctataca ccctatcgca
      541 cattggtgaa agagtgtttt ccgaatgccc gaatcgtcgc cgatcgcttt catattagcc
      601 aacacatcgg tcgggccttt accaaccacc gaattcaagt aatgaagacc tttaaaaaag
      661 gggatcgtcg gtcgaagcac ttaaagaaat actggagact acttcaaaag aatgcgtggg
      721 aattgaatgg tcagcatcgc tactggcgtc cgtcatttcg agatcatctg acagaagcgg
      781 agatagtcga tcggctatta tattatgatg actccttaaa gcgaggctac gaagtctacc
      841 aggtttttct ttccgctatc agaagacaag atgttcctga attcgtcgcg ctcctaaaag
      901 aagactacaa ggagttgcca gagcactatc aaccagtgtt cacgaccttc aagaaatatc
      961 gaacagaaat caaacgagcg ttgcgagttc cctattccaa cggccctata gaatgtttga
     1021 acaaccatat caaagtacta aaacgaattg cctatggctt tagaaacttt caaaactacc
     1081 gagaacgaat ctttttatat cgaggaaaat atttcaagaa aactaagaac actacccaac
     1141 tgactaaagc cagaacaaca acacggctag acaagatagc tgtctagccg tgtgagtatc
     1201 tcgattattc tattgaattt cttcacca
//
LOCUS       DAZUKT010000212         1211 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000212 DAZUKT010000000
VERSION     DAZUKT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.225"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..670
                     /locus_tag="ACG816_003118"
                     /pseudo
     CDS             1..670
                     /locus_tag="ACG816_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
     gene            996..1109
                     /locus_tag="ACG816_003119"
     CDS             996..1109
                     /locus_tag="ACG816_003119"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HFS4921909.1"
                     /translation="MGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          455 a          180 c          183 g          393 t
ORIGIN      
        1 ctgtttcatg cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg
       61 acacactacc gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac
      121 aacagtatca gcgctcaatc catgatcgac ctgataatta tcctcatttc aattctgagt
      181 ttggtcacct tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt
      241 tagttgaaag attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag
      301 atattgaaac tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta
      361 ttacgctatt acgtttgact gtggaaaaga attttctaac tggaaagcca ttagtaacca
      421 acatgatatt gatatatatt ttgcggaccc tggaacacct tctcaacgcc cattaaacga
      481 gaattctaac gggattctgc gtcgtaatgg actgccgaaa tcaatggatt ttagagaagt
      541 gaatcagaca tttatttcca gtgtcagcaa tcaacgtaat catattccaa gaaaatcatt
      601 gaattacaga acaccaatcg aaatattttt gagctatgta caagaagcat tttattctag
      661 cttaatttga caaatcataa ataataaaga attgaacttc tgagaaaaca aagaatcttt
      721 tttctttttt tgaattgact catggattgg cgttaaaatt ttattttgaa aaagttattt
      781 ctggtttaac tcaaaaaaag agatatgaat ctaatagtga tagaaaggat gttatttttc
      841 atgaaatggt tgttttagat ttaatacata taactaataa atgctattga atgtatttaa
      901 actaattaac aaaagaattt gaattgttaa taaaatccaa taaaatatat taataaagta
      961 gatagaaata aaaaaacaaa gactaaacat atataattgg attaaacgca atatctgcaa
     1021 aatattgtaa atttaaagcc tttgtagaaa aatttactga aagacaaact gataagatta
     1081 ttcagtatca tattacctat actgattaaa attttttgaa acgggatact aaataaaaaa
     1141 tttatttata acagaactaa ttatttaatg cggatgcgcc aatacttggt cctgcaagag
     1201 ctatttattt t
//
LOCUS       DAZUKT010000213         1166 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000213 DAZUKT010000000
VERSION     DAZUKT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.226"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1147
                     /locus_tag="ACG816_003120"
     CDS             20..1147
                     /locus_tag="ACG816_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4921910.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          421 a          201 c          225 g          319 t
ORIGIN      
        1 caattggagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcga
       61 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
      121 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      181 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      241 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      301 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      361 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      421 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      481 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      541 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      601 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      661 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      721 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      781 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      841 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      901 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      961 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
     1021 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1081 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1141 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAZUKT010000214         1136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000214 DAZUKT010000000
VERSION     DAZUKT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.227"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1061)
                     /locus_tag="ACG816_003121"
     CDS             complement(<1..1061)
                     /locus_tag="ACG816_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921911.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          301 a          254 c          182 g          399 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaac
//
LOCUS       DAZUKT010000215         1095 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000215 DAZUKT010000000
VERSION     DAZUKT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.228"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG816_003122"
     CDS             127..579
                     /locus_tag="ACG816_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921912.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..>1095
                     /locus_tag="ACG816_003123"
     CDS             593..>1095
                     /locus_tag="ACG816_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921913.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYID"
BASE COUNT          387 a          145 c          218 g          345 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacga
//
LOCUS       DAZUKT010000216         1079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000216 DAZUKT010000000
VERSION     DAZUKT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.229"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1079
                     /locus_tag="ACG816_003124"
     CDS             <1..>1079
                     /locus_tag="ACG816_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921914.1"
                     /translation="KIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINRALSL
                     ESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLF
                     ICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKL
                     YDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKA
                     AIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSY"
BASE COUNT          340 a          150 c          142 g          447 t
ORIGIN      
        1 aaaaattttt tctgtaaaaa tatttatagt ttatgcattt gttagtttat tatatttatt
       61 tccagcattt aaaaacatgg aaatatttgg cttttatgat acggcatttc acattaatcg
      121 tgcgttaagt ttagaatcta tttttagctc ccctattaat tttgaaacgt ttagatcata
      181 tggcatgcaa gtaaataatt tttacccatg gttaactcta tatcctttat ttttattgat
      241 caagtttact aatttagcaa taggctataa cctttttctt tatattgtta cgctcattac
      301 tctatttata tgtcattatg taatgtatga aattaccaaa aaacatgtta ctagctcctt
      361 tttcgctatt atatatacaa catcttcttt taggagtgta gaaatatttc ttcgaggggc
      421 aatgggggaa ttactcgcaa tgtcaatact tcctcttatt ttactcgggt ttataaagct
      481 ttacgactca aaaaaagaat cttgggttat gttggcgatt agcatgactt tattaattta
      541 cactcatgtt ctttctgtag ctatgactat ggtaatgatt ataattgctt taattattca
      601 attttatcgg aaaaagaaaa tagaaatctt cttaattatt aaattaataa aagctgctat
      661 agttacttta cttctttcac tagcgtttat aggtcctatg attgagcaga cattgtatca
      721 agatctgaat agaccttatg tggatatatt acaaaaaaga gcaatttatc tgggtaaaga
      781 gtttttgatt ggaagtatag attcagaatt actatcattt ggtattggct taactttgct
      841 tatttgttta attatactaa catttaactt taaaaaatta ggaatcttat ctaaaatcac
      901 ttatataatg ggtataatat ctattctact ttgtacaaaa atatttccat ggtttgcctt
      961 acaaaatact cctttaaata ttattcaagc tccttggcgt tttatgatat tttcaacatt
     1021 gttcatatct ttttcagtat ctctatttat ttctccacag ttggaaaaat tttcttata
//
LOCUS       DAZUKT010000217         1060 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000217 DAZUKT010000000
VERSION     DAZUKT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.230"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..994)
                     /locus_tag="ACG816_003125"
     CDS             complement(38..994)
                     /locus_tag="ACG816_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921915.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DAZUKT010000218         1045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000218 DAZUKT010000000
VERSION     DAZUKT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.231"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG816_003126"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG816_003126"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4921916.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAZUKT010000219         1023 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000219 DAZUKT010000000
VERSION     DAZUKT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.232"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1023)
                     /locus_tag="ACG816_003127"
     CDS             complement(<1..>1023)
                     /locus_tag="ACG816_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFS4921917.1"
                     /translation="SIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSA
                     KTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRM
                     NFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKAR
                     LKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHS
                     ENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAY
                     HFCQLLRYYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLN"
BASE COUNT          284 a          199 c          226 g          314 t
ORIGIN      
        1 tattcagccc ctcagtgacg ccgtttgagt ggggcacctt aaaggcattt gaaatacccg
       61 attggtagcg atggaagatc gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa
      121 ttgttttaag ggtttcttga aagccttccg tatctcgttt aacgaaataa tagcgtaata
      181 attgacaaaa atgatacgca gttcgcaatt cttcgttgta gcttaacagc tcatcaacga
      241 tttctgtttg actaattggt cgtttaaaca gtcgatgata atgccgtttg agtggctcca
      301 atgtcgtgga atctttgagt aagagtttcc aatatcgctt gagccgacgg tacttcttct
      361 gattttcaga gtgtgaggtt cgaaaacgat tcatgatctg gacacgtaat tgattgaagc
      421 tgcgattgat gtgttggaca atatgaaagc ggtcagttac gatctccgca tttggaaaga
      481 ctgttttaag caactgacaa tagctggcat tcatgtccat cactaggtac ttgaccttta
      541 aacgggcctt tctaggaaag cgcaggaagt attgagttag tttgtaaagt ctgcgatctt
      601 caaggatcgt aaatagctga ttggttgtcc catcaacaca aataaaactc atggaaccag
      661 aaacagactt cattgatttg aactcatcca tacacaacac cgtcggaagg aagttcatac
      721 gtggctggta atgcttcgtt gcttcacgca agatccgtgt caccgtccca tccgagacga
      781 agtacttctt agcgattagt ttacgtgatt gattttcgta cagttccatg agaatcgcct
      841 gcttcagttc ctttgttagt tgatgatgtt catcaactaa gtccgttttc gcagaaaagg
      901 tccgtccaca atcatggcag agaaaacggg tacgttttaa ttctaaaata gtcaactttt
      961 ctttgacttt caacatctga gtgcgtgtcg tataggaacc gttgcggata atacgaatcg
     1021 atc
//
LOCUS       DAZUKT010000220         1010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000220 DAZUKT010000000
VERSION     DAZUKT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.233"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1010)
                     /locus_tag="ACG816_003128"
     rRNA            complement(<1..>1010)
                     /locus_tag="ACG816_003128"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          211 a          318 c          227 g          254 t
ORIGIN      
        1 ggggcatgat gatttgacgt catccccacc ttcctccggt ttgtcaccgg cagtcttgct
       61 agagtgccca actgaatgat ggcaactaac aataagggtt gcgctcgttg cgggacttaa
      121 cccaacatct cacgacacga gctgacgaca accatgcacc acctgtcact ttgcccccga
      181 aggggaagct ctatctctag agtggtcaaa ggatgtcaag acctggtaag gttcttcgcg
      241 ttgcttcgaa ttaaaccaca tgctccaccg cttgtgcggg cccccgtcaa ttcctttgag
      301 tttcaacctt gcggtcgtac tccccaggcg gagtgcttaa tgcgttagct gcagcactga
      361 agggcggaaa ccctccaaca cttagcactc atcgtttacg gcgtggacta ccagggtatc
      421 taatcctgtt tgctccccac gctttcgagc ctcagcgtca gttacagacc agagagccgc
      481 cttcgccact ggtgttcctc catatatcta cgcatttcac cgctacacat ggaattccac
      541 tctcctcttc tgcactcaag tctcccagtt tccaatgacc ctccccggtt gagccggggg
      601 ctttcacatc agacttaaga aaccgcctgc gctcgcttta cgcccaataa atccggacaa
      661 cgcttgccac ctacgtatta ccgcggctgc tggcacgtag ttagccgtgg ctttctggtt
      721 agataccgtc aagggatgaa cagttactct catccttgtt cttctctaac aacagagttt
      781 tacgatccga aaaccttctt cactcacgcg gcgttgctcg gtcagacttt cgtccattgc
      841 cgaagattcc ctactgctgc ctcccgtagg agtttgggcc gtgtctcagt cccaatgtgg
      901 ccgatcaccc tctcaggtcg gctatgcatc gtggccttgg tgagccgtta cctcaccaac
      961 tagctaatgc accgcgggtc catccatcag cgacacccga aagcgccttt
//
LOCUS       DAZUKT010000221         1000 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000221 DAZUKT010000000
VERSION     DAZUKT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.234"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1000
                     /locus_tag="ACG816_003129"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ACG816_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAZUKT010000222          999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000222 DAZUKT010000000
VERSION     DAZUKT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.235"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..635)
                     /locus_tag="ACG816_003130"
     CDS             complement(81..635)
                     /locus_tag="ACG816_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4921918.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          186 c          107 g          383 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctacatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaagt ttgaacaata tactaagaac tccccttact ataaagtgaa
      961 ataaattagc atatgaataa ccgtgctacc aaacttttc
//
LOCUS       DAZUKT010000223          982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000223 DAZUKT010000000
VERSION     DAZUKT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.236"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..591
                     /locus_tag="ACG816_003131"
     CDS             <1..591
                     /locus_tag="ACG816_003131"
                     /inference="COORDINATES: protein motif:HMM:NF015839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HFS4921919.1"
                     /translation="KSEQDRNERTNILMKRLEASTSNFNNKLDVFEEKTKHARLNFET
                     TAKHYIKRLDEDNLKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDH
                     NKVVDKSNTALKVMTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDH
                     ESAWRYLMLILYAVPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          376 a          136 c          188 g          282 t
ORIGIN      
        1 aagagcgagc aagacaggaa cgagagaaca aacatactca tgaaaagact agaggcttcg
       61 acctcgaact ttaataataa gttagatgtt tttgaagaaa agactaagca tgcaagatta
      121 aactttgaaa caactgcaaa gcactatatt aaacgacttg atgaagataa tttgaaatta
      181 gattttcaac aagctattca agatgaatta agcgatacta aagacgaaat acgtgaagtg
      241 acgaaacaag cgagagaaga aacaaaagag tataaagaaa tcttagaaag taaaataaaa
      301 gaccataaca aagtggttga taaatcgaat acagcgctaa aagtgatgac aaaaggcgtt
      361 acaaatatat tttttgtgct aattattttt gtacttgtaa tgcttgtgac aggtccgata
      421 ggtcatttct ttggtattga acatttatat agctttatta atggttttat tgatgaccac
      481 gaaagtgcat ggcgatattt gatgttgata ctttatgcag taccctatgt attcttcgct
      541 atcattctgt ggctcatagc attgtttttt agagtattta atgaccttta ggtagattta
      601 tatataaaaa gggctaaata cgcaaaatac ccccttaaaa tgcaaaataa atgcaaataa
      661 gggggtaact ccttatgaaa aaacaactaa tagtattata acacgttttt atgccgagaa
      721 aacttattgg ttggaatggg ctatgtgtta gctaacttgt tagcgagttg gttggacttg
      781 aattgggatt aatcccaaga aagtaccaac tcaacaacac ataaagccct gtaggttccg
      841 accaataagg aaattggaat aaagcaacga aaggagttga gtaaaatgag tgagaaaacg
      901 aagaaaatca ttcgagtatc attagctttt tcacaagtag tagtgcaatc attgatagtt
      961 tattatactt accagcaata ta
//
LOCUS       DAZUKT010000224          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000224 DAZUKT010000000
VERSION     DAZUKT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.237"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG816_003132"
     rRNA            <1..>943
                     /locus_tag="ACG816_003132"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZUKT010000225          917 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000225 DAZUKT010000000
VERSION     DAZUKT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.238"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(602..675)
                     /locus_tag="ACG816_003133"
     tRNA            complement(602..675)
                     /locus_tag="ACG816_003133"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
BASE COUNT          310 a          154 c          160 g          293 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcac
//
LOCUS       DAZUKT010000226          909 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000226 DAZUKT010000000
VERSION     DAZUKT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.239"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..876
                     /locus_tag="ACG816_003134"
     CDS             175..876
                     /locus_tag="ACG816_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4921920.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          330 a          160 c          147 g          272 t
ORIGIN      
        1 caaatcattc gcttcaaaac aaattattca ataccattgt atatttttta cataataaca
       61 aattatattt gtaaagtatt aaaacttgtt tctaatgttg gtatgttaaa actttacaaa
      121 ttctttaaaa aaagtgacaa taaatatagt gaacaataag gagaggactt tttaatgcgt
      181 gttaataaaa tcgattttca cttttcaaaa ctcagatatc aacctgattt tgaacatttc
      241 tcggatgaag agttccaaca gatcaaggaa catactgttc ttcgttctta taaaaaagga
      301 cagatccttt tcgacgatgg tgatcccaga caacggttgt atatcttgac caaaggactc
      361 gttcgtttag aacgttatga tgaaagtgca acttattact actatgacta tgtgaataca
      421 aataaccttt ttcctatgtc aggaatgttt ggagaagaga cctacactta tacagctcaa
      481 gcgatgactg atattgaaac attctacctt cctgtttcct actatgaaaa attagcacga
      541 aaaaatcccg aacagttgat ctatatgata caaaaaattt ctaaagtagt tgaaatacat
      601 gaattgcgta tccaaatcgg attgacctct agcgcctttg accgggtaaa acaaaattta
      661 ttcattttga aagaagaatt gggcgtacca acaaaagatg gtcatatcac cattccttat
      721 ccgatcacct taaaagaatt agcagtgaat agcggcacga cacgtgaaac tgctggacaa
      781 gtaatcaaac aactacgtga ttccgggctg ttagattatg acaaaaagcg ttttactttc
      841 tgtaagacag ctactactcc agctatgaga gaataacgcg aacaaaaata agcagtgacc
      901 ctgataaat
//
LOCUS       DAZUKT010000227          876 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000227 DAZUKT010000000
VERSION     DAZUKT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.240"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..621
                     /locus_tag="ACG816_003135"
     CDS             <1..621
                     /locus_tag="ACG816_003135"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4921921.1"
                     /translation="YVDIIGYDTYDKINHAYAFGGVAMTINTIQAYLDIPIDFFVTLN
                     MQGLEQIVDSIGGITLTPTATFTQDSYSFYQGEIINLDGKASLAYSRNRHADDSGDYG
                     RQARQREIIMAIIEKGTKVTTLATKIPSLIKSLQGNMTTNLSVDDMWTIINKYSTALG
                     NIEQVQLKGEGQLLDDGIYYEMINEESLEEVQNILQKQLDYHLNID"
     gene            695..>876
                     /locus_tag="ACG816_003136"
     CDS             695..>876
                     /locus_tag="ACG816_003136"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921922.1"
                     /translation="MKKSRHIKGISQSEVAKYLNISRQSISKWENDTSSPDINNLTLL
                     SSLYEISIDDLLKEKK"
BASE COUNT          340 a          130 c          146 g          260 t
ORIGIN      
        1 tatgtcgata ttatcggtta tgatacttac gacaaaatta accatgccta tgcctttggt
       61 ggagtagcca tgactataaa tacaatacaa gcatacttag atatacctat tgactttttt
      121 gtaacgttaa atatgcaggg tctagaacaa attgttgatt ctattggagg aataactctt
      181 actccaactg ccacttttac acaagatagt tattcatttt atcaaggcga aattattaac
      241 ttagatggga aggcttcctt agcgtactct cgaaatcgtc atgcagatga ttcaggtgac
      301 tatgggagac aagctcgtca aagggaaata ataatggcaa ttatagagaa aggaactaaa
      361 gttactacac tagcaactaa gataccttca ctcattaaga gtcttcaggg aaatatgact
      421 actaatctgt cagttgacga tatgtggact attattaata aatattccac tgctttagga
      481 aatatagagc aagttcaatt aaagggggaa ggacaactac ttgatgacgg aatatactat
      541 gaaatgatta acgaagaatc tttagaagaa gttcagaata tccttcaaaa acaactagat
      601 tatcacttga atatagacta aataatctat ttcttaatat ccaataaata aaagtcatta
      661 ataggagagg atacagaatg acattagggg aaaaattaaa aaatcacggc atataaaagg
      721 catctctcaa agtgaagtag caaaatattt aaatatatct agacaatcta tttctaaatg
      781 ggaaaatgat actagttccc cagatataaa caacttaact ttattaagta gtttatatga
      841 aatctctatc gatgatcttt taaaagaaaa aaaaga
//
LOCUS       DAZUKT010000228          842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000228 DAZUKT010000000
VERSION     DAZUKT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.241"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>842
                     /locus_tag="ACG816_003137"
     CDS             <1..>842
                     /locus_tag="ACG816_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4921923.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLK"
BASE COUNT          367 a          108 c          136 g          231 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 tt
//
LOCUS       DAZUKT010000229          831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000229 DAZUKT010000000
VERSION     DAZUKT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.242"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..482)
                     /locus_tag="ACG816_003138"
     CDS             complement(84..482)
                     /locus_tag="ACG816_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4921924.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(479..715)
                     /locus_tag="ACG816_003139"
     CDS             complement(479..715)
                     /locus_tag="ACG816_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4921925.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          260 a          143 c          124 g          304 t
ORIGIN      
        1 attttgtatg attaaacata aaaaaatcag catattcctt aatgacatgc tgatctaatt
       61 gagaaataaa gtttttaatt cgttcatttt attgcggttt gtcgtaaata actggttatc
      121 ttggacttta gtgaatcgaa atccaatgaa ctagtggtaa tcattatgat aaaatcaaca
      181 gcttcttgac gagaaaaagc aactggacaa ttattcaatt gaagaaacaa caacatggca
      241 acaagagcag ttcgtttatt gccattatga aaaggatgtt ttttagctaa attaattgct
      301 aaaatcgccg ctttttcata gatttcagga tagagttctt gattgaatac tgcttgtgaa
      361 ggctgtttga cggccatatc taatgcattt gcatctttta caccgatcat ttcgcctggc
      421 gagtgacgta aaataacttt cgcgttgatt ttgataattt ctttagtcga aagatacctc
      481 ataaatcaac caaatctttt aacgcgtctt catattgatt catgatcgaa tcaactaatg
      541 aatcaatatc aggttcttct tgtgctttga tcattcgtac agaaccatca gattgaaaaa
      601 cataactgat tgcgtcacct gtctgcacac ccatgaattc ttttacttct ggtgggatag
      661 ttgttacatc gctattgcca gattttctaa ttttatactt tttcattttt ttcatatttc
      721 gccaccctct ataccattct cattctatag taagtacatt atatccaagt atatactttt
      781 aatcaatgta tatactttta ttttgctttt tcttacgaaa aaatgacaaa g
//
LOCUS       DAZUKT010000230          791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000230 DAZUKT010000000
VERSION     DAZUKT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.243"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACG816_003140"
     CDS             <1..716
                     /locus_tag="ACG816_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4921926.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DAZUKT010000231          748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000231 DAZUKT010000000
VERSION     DAZUKT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.245"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG816_003141"
     CDS             complement(<1..210)
                     /locus_tag="ACG816_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4921927.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="ACG816_003142"
     CDS             complement(309..623)
                     /locus_tag="ACG816_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921928.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
BASE COUNT          253 a          144 c           80 g          271 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttgg
//
LOCUS       DAZUKT010000232          717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000232 DAZUKT010000000
VERSION     DAZUKT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.246"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..314
                     /locus_tag="ACG816_003143"
     CDS             102..314
                     /locus_tag="ACG816_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4921929.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            304..699
                     /locus_tag="ACG816_003144"
     CDS             304..699
                     /locus_tag="ACG816_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921930.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          254 a           87 c          137 g          239 t
ORIGIN      
        1 aaaatttata tggaaagctt ccttgacatt ttgtgctcgc aggagtatta taaaagcacg
       61 aaaggaaagt aaactttcca ttttttagaa aggagcatgt tgtgaaaaat cgtttggaag
      121 aattacgaaa acaaagagga attaaacaag aagatttagc ttctgctctt gaagtatctc
      181 gtcaaacaat aggttcttta gaaaatggac gttataatcc ctctattata ttggcattta
      241 aaattgcaag gtactttcaa atgagcattg aagaaatttt tatctatgag gaggaaagtg
      301 aagatgaaaa ataagggtca gatttggtat atcggttata ttgtttcgtt gtttttgatt
      361 gtgacaattt tggttacaga ttttcctaaa atggtagata ttggacttgc tattttattc
      421 agtgccgttt ttggcgtttc tcatgttcaa attttgcatg ataaaatgat taaaaatgat
      481 gcagactaca aaatcaatgt tatggacgaa cggaatatca taatcaaaga aaaagcggga
      541 aatataacaa atatgtttaa cacaatactg ttaggggtgg caacagttat atttatttgt
      601 ttagactaca tcattccagc tattattgtc ggaagtatca ttgcaatcca gccgattatt
      661 ttaatcatta tcagtagtgt gcttgagaaa aaaatgtaga aataaaggtt catacaa
//
LOCUS       DAZUKT010000233          706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000233 DAZUKT010000000
VERSION     DAZUKT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.247"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..258
                     /locus_tag="ACG816_003145"
     CDS             <1..258
                     /locus_tag="ACG816_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921931.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="ACG816_003146"
     CDS             260..397
                     /locus_tag="ACG816_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4921932.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            435..>706
                     /locus_tag="ACG816_003147"
     CDS             435..>706
                     /locus_tag="ACG816_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921933.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          273 a          100 c          142 g          191 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaagaagc
      361 agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt
      421 caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga tcaataaccg
      481 ctatcgtttc tttgatttta ttagtaatta gaccgattgc tgcatctttc tcgaaaatta
      541 ctgagactct ttcaaaagta agccacaatt tagatttgct gactaaagat ttagaatcga
      601 gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata
      661 cacatacaga aaaattggtg gaacacacgc aacaaatcaa aacttt
//
LOCUS       DAZUKT010000234          692 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000234 DAZUKT010000000
VERSION     DAZUKT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.248"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>692
                     /locus_tag="ACG816_003148"
     CDS             <1..>692
                     /locus_tag="ACG816_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS4921934.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWH"
BASE COUNT          233 a          125 c          145 g          189 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac at
//
LOCUS       DAZUKT010000235          664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000235 DAZUKT010000000
VERSION     DAZUKT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.250"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          254 a           84 c           83 g          243 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaataag aactaaatag gaaaacgtag acgtaatttt
      121 ctacgttttc cttttcatat atattaaata ttacggaaaa ttatataaac taatttagtg
      181 ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc tatttaactc
      241 attagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt acttaatttt
      301 gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat gatatatatc
      361 tacagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa acctataaaa
      421 tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa attatggatc
      481 acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta ttgattgcaa
      541 aattatgtga caaaaagaac tagtagaaga aggaataatg gcttgataat aaagtactac
      601 ctattaaagg tagtacttta tttaatttag tataaattaa catactacta tattttaggt
      661 ttca
//
LOCUS       DAZUKT010000236          649 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000236 DAZUKT010000000
VERSION     DAZUKT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.251"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>649
                     /locus_tag="ACG816_003149"
     CDS             <1..>649
                     /locus_tag="ACG816_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4921935.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAZUKT010000237          624 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000237 DAZUKT010000000
VERSION     DAZUKT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.252"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..515
                     /locus_tag="ACG816_003150"
     CDS             <1..515
                     /locus_tag="ACG816_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4921936.1"
                     /translation="KLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVM
                     NQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKAD
                     WYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTE
                     GLRLHSMGLA"
BASE COUNT          230 a           99 c          121 g          174 t
ORIGIN      
        1 agaaactaaa gcgtgaacaa cgcaaattgt ctaggcgtgc tttattggct aaaaataaag
       61 gtaccaatct atttgaagct aaaaattatc aaaaacaaaa acgcaaagta gctagattgc
      121 atgaaaaagt aatgaatcaa cgtactgatt ttctgaataa gttgagtaca gaaatgatca
      181 aaaatcacga tattatctgt attgaagact taaacacaaa aggtatgttg cgaaatcata
      241 aattagctaa aagtatttct gatgtttctt ggtcaaagtt tgtgactaaa ttacaataca
      301 aggctgactg gtatggacga aaaatcatca aagtagataa atggtttccg tctagtcaga
      361 tttgttcaga gtgcggacat aaagatggca agaaatctct tgaaattcga gaatggactt
      421 gtcctgtttg tcatactcat catgatcgtg atatcaatgc tagtatcaat attctgaccg
      481 aaggtctaag actacattct atgggattgg cttagatcca atcatcaaga accgtaggaa
      541 ctacggggat agcttggtaa ataagagaca ccgctgttag taaagaaata cgctatcaag
      601 tatgctctat tcccaagaag ctcc
//
LOCUS       DAZUKT010000238          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000238 DAZUKT010000000
VERSION     DAZUKT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.253"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG816_003151"
     tRNA            complement(65..150)
                     /locus_tag="ACG816_003151"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG816_003152"
     tRNA            complement(167..238)
                     /locus_tag="ACG816_003152"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG816_003153"
     tRNA            complement(250..322)
                     /locus_tag="ACG816_003153"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG816_003154"
     tRNA            complement(340..421)
                     /locus_tag="ACG816_003154"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG816_003155"
     tRNA            complement(429..501)
                     /locus_tag="ACG816_003155"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG816_003156"
     tRNA            complement(505..577)
                     /locus_tag="ACG816_003156"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZUKT010000239          558 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000239 DAZUKT010000000
VERSION     DAZUKT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.254"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>558
                     /locus_tag="ACG816_003157"
     CDS             <1..>558
                     /locus_tag="ACG816_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS4921937.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRTASTTIKGMEA"
BASE COUNT          193 a           99 c          110 g          156 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat
      541 taaaggcatg gaagccat
//
LOCUS       DAZUKT010000240          557 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000240 DAZUKT010000000
VERSION     DAZUKT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.255"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>557
                     /locus_tag="ACG816_003158"
     CDS             <1..>557
                     /locus_tag="ACG816_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4921938.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCG"
BASE COUNT          205 a           88 c           97 g          167 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtggaca
//
LOCUS       DAZUKT010000241          534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000241 DAZUKT010000000
VERSION     DAZUKT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.256"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG816_003159"
     CDS             <1..>534
                     /locus_tag="ACG816_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4921939.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZUKT010000242          513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000242 DAZUKT010000000
VERSION     DAZUKT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.257"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>513)
                     /locus_tag="ACG816_003160"
     CDS             complement(107..>513)
                     /locus_tag="ACG816_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4921940.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          102 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttc
//
LOCUS       DAZUKT010000243          510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000243 DAZUKT010000000
VERSION     DAZUKT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.258"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG816_003161"
     CDS             complement(<1..434)
                     /locus_tag="ACG816_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921941.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT          146 a           85 c           80 g          199 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DAZUKT010000244          508 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000244 DAZUKT010000000
VERSION     DAZUKT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.259"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          227 a           70 c           81 g          130 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactct
//
LOCUS       DAZUKT010000245          501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000245 DAZUKT010000000
VERSION     DAZUKT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.261"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          170 a           96 c           58 g          177 t
ORIGIN      
        1 aaaaataaga atgctaaatc actctacaat aacggcgaaa gcacaaaaaa taaaaaaatt
       61 attacgttat taaaaaaaca gatgaaagat caggttttat tagatctcat tctttcatca
      121 cttgaaggga tttaatgaac tactttttcc tctttgtcgt ttttatatat tcgtattcct
      181 tcaaacagta ggatagcctc tttcaatgct tgatatttga actgtccgtt acttaaaaat
      241 ttagatgtgc cgccctcttc ctagaacata ttctttttta ctttttcatt aattgacaga
      301 ccaatcgaac gaaactgtaa atttcgatag gataatgatt ggtcaaactc attcattttc
      361 aagacatctc gattcatatt caagatagga atctttttat caacatcact ttattcgtat
      421 tttcctctac cataaaagct tcacgctcca caaaattgtt ttatacacta ctattcagcc
      481 cattcaacga ccaatgaatt t
//
LOCUS       DAZUKT010000246          487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000246 DAZUKT010000000
VERSION     DAZUKT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.262"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACG816_003162"
     CDS             complement(<1..>487)
                     /locus_tag="ACG816_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921942.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAZUKT010000247          475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000247 DAZUKT010000000
VERSION     DAZUKT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.264"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..>475
                     /locus_tag="ACG816_003163"
     CDS             21..>475
                     /locus_tag="ACG816_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921943.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          177 a           87 c           95 g          116 t
ORIGIN      
        1 tacaaggaga gtgaacataa atgacttata cccatcttac accaaacgag cttgtaatga
       61 tagaagcata ttttcatcaa gaaactccgg ttgctatcgt tgcgaagcag cttaaacgtg
      121 gacgccaaac aatttacaat gtctataact ttctcaaatg tggtggaaca gcacttgaat
      181 actttgaaca atacaaagaa aataagcgac gttgtgggag aaccgaaatc atttttcctg
      241 ctgaggaaaa agaatacatt gaaaaaagat caactgaagg ttggacccca gacgtcatta
      301 ttggccgtgc agagcgaacc ttctcttgtt cagtaagtac cctctatcgc cggtttaaga
      361 cgggagaatt caatgtttta catttaccga tgcaaggaaa acgaaaacca aatggttata
      421 aggaaaaacg tggaaaacag gcattcaaaa gaaatatttc tgaacgtaaa aaaga
//
LOCUS       DAZUKT010000248          472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000248 DAZUKT010000000
VERSION     DAZUKT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.265"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..109
                     /locus_tag="ACG816_003164"
     CDS             <1..109
                     /locus_tag="ACG816_003164"
                     /inference="COORDINATES: protein motif:HMM:NF013850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS4921944.1"
                     /translation="LYCNKEKCWVNWGQSWSEGLKRWGDNLFGSFSGGR"
     gene            109..459
                     /locus_tag="ACG816_003165"
     CDS             109..459
                     /locus_tag="ACG816_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4921945.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          193 a           50 c           77 g          152 t
ORIGIN      
        1 tctttattgt aacaaagaaa aatgttgggt aaattgggga caatcatggt cagaagggtt
       61 aaaacgttgg ggagataatc tttttggtag ttttagtgga ggacgataat gacttcaata
      121 aacattgatc acacaaaaaa agtcatacat gaattataca atagtttaat gacacgacct
      181 aatcagacga aagcattatt agatattact gatgttttat ttcaggtata taaaaaaatt
      241 gatacagcaa aatatcctga aattcttgta aataaattag ttaattatat ttacatcgtg
      301 ggttttgata ataaaatacg ctttattgat aatgatgaaa aattattaat agaattaggg
      361 gatatttcta aaaaagctgg aattaatagt aaatacaaag caaactttac agataaatcg
      421 caattttata gttattcaga aacaatgcct caacgataaa atatagtata ct
//
LOCUS       DAZUKT010000249          470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000249 DAZUKT010000000
VERSION     DAZUKT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.266"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>470
                     /locus_tag="ACG816_003166"
     CDS             <1..>470
                     /locus_tag="ACG816_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFS4921946.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DAZUKT010000250          462 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000250 DAZUKT010000000
VERSION     DAZUKT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.268"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG816_003167"
     CDS             complement(<1..430)
                     /locus_tag="ACG816_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4921947.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZUKT010000251          429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000251 DAZUKT010000000
VERSION     DAZUKT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.270"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          173 a           55 c           60 g          141 t
ORIGIN      
        1 agcgctaatc taattcactg tattatcatt ttttagtagt atagcacaat tatgaatgcc
       61 ttttctttat atcaaataaa gtttatataa aaaacctcat cctgcaagaa agaaaaaatg
      121 aacccattct ttttatacta ggtcttaaaa agacagaaca tctgctagaa gatagaagag
      181 aataagagtt atatatgttg gaagatattc ttagaaaaaa gacaaaactt ttagaacacg
      241 aaaactatat tgaacgaata aaatgaaaaa atgattaaaa aatcatttta ttatttacgt
      301 ttacattcta atatgttaaa ttgaatataa cttaaacagg catgtgactg gtaatgcagg
      361 caggacctaa ataagttgaa taacaatgta tgcgtttcaa tacacgtttg ccttgttgtc
      421 aatttattt
//
LOCUS       DAZUKT010000252          415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000252 DAZUKT010000000
VERSION     DAZUKT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.271"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>415)
                     /locus_tag="ACG816_003168"
     CDS             complement(<1..>415)
                     /locus_tag="ACG816_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921948.1"
                     /translation="VFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFS
                     LLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRY
                     NYKLSVFSIISLAELIFSLTTPGNALRAAHEINKW"
BASE COUNT          153 a           69 c           53 g          140 t
ORIGIN      
        1 aaccatttat taatttcatg tgcagcacgt aatgcattcc ctggagttgt caaagagaaa
       61 attaattctg ctaagcttat tattgaaaat actgataact tataattata tcgtccattg
      121 acaatcaaat ataaactaac tatacttgtt agaacaaaaa aacaggcgtt gacttgttct
      181 tgattagtta aaaatatcaa taaaggaata cttacccaat atatttttct atttatttct
      241 tctcctctta agagttgaaa aaacggataa aaagttagta aactaaatgc tactggccac
      301 tgatagttca aggtggttgc tctccaaccg gtttccccca ttactgtcaa tggtattaaa
      361 cagtatatag agcaaaccaa taataaattt ttaacattta ttttctcaaa aacat
//
LOCUS       DAZUKT010000253          385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000253 DAZUKT010000000
VERSION     DAZUKT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.272"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..>385
                     /locus_tag="ACG816_003169"
     CDS             12..>385
                     /locus_tag="ACG816_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921949.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGS"
BASE COUNT          130 a           52 c           95 g          108 t
ORIGIN      
        1 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa
       61 aaacgcatgt agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag
      121 ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga
      181 cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga
      241 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
      301 aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat cggacaccaa
      361 ttagttttga acaactggtt gggtc
//
LOCUS       DAZUKT010000254          359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000254 DAZUKT010000000
VERSION     DAZUKT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.273"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>359
                     /locus_tag="ACG816_003170"
     CDS             <1..>359
                     /locus_tag="ACG816_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS4921950.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKG"
BASE COUNT          134 a           59 c           77 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaagg
//
LOCUS       DAZUKT010000255          335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000255 DAZUKT010000000
VERSION     DAZUKT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.274"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          125 a           54 c           59 g           97 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaata
//
LOCUS       DAZUKT010000256          323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000256 DAZUKT010000000
VERSION     DAZUKT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.275"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG816_003171"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG816_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS4921951.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZUKT010000257          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000257 DAZUKT010000000
VERSION     DAZUKT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.276"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG816_003172"
     CDS             complement(<1..>321)
                     /locus_tag="ACG816_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921952.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZUKT010000258          319 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000258 DAZUKT010000000
VERSION     DAZUKT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.277"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..269)
                     /locus_tag="ACG816_003173"
                     /pseudo
     CDS             complement(33..269)
                     /locus_tag="ACG816_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           98 a           49 c           55 g          117 t
ORIGIN      
        1 gaaggactga ccttctgtct cagatttttt tgttatttca cttgaatcaa ttgattgatc
       61 aaaaaaatcc tgattttcat gttctcaaat gatttaaatc cataagataa tcgtttcagt
      121 gtcttgatat ggggaatctt ggcctctatt ttgccattag aatacgggta aatcatagca
      181 ttgtaaatac cttcttcata cgttaaaagg ttttgaagtt tcgtgcgaag ttgtgcatca
      241 agtgtttcag gaagattttt taataacgcc gtttgtataa ttaagcgaga caaataaaaa
      301 agccatttat gttacactc
//
LOCUS       DAZUKT010000259          312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000259 DAZUKT010000000
VERSION     DAZUKT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.278"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..193
                     /locus_tag="ACG816_003174"
     CDS             <1..193
                     /locus_tag="ACG816_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4921953.1"
                     /translation="EAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLV
                     DTLLRKHQLYTPPRSVIEK"
BASE COUNT          113 a           66 c           49 g           84 t
ORIGIN      
        1 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
       61 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
      121 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
      181 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
      241 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
      301 aatttcatct tg
//
LOCUS       DAZUKT010000260          304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000260 DAZUKT010000000
VERSION     DAZUKT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.279"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>304
                     /locus_tag="ACG816_003175"
     CDS             <1..>304
                     /locus_tag="ACG816_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4921954.1"
                     /translation="LFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSA
                     DVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIE"
BASE COUNT          124 a           35 c           55 g           90 t
ORIGIN      
        1 tctatttgtt aaaaatagaa ctgtaaaaac tacaactatt cagttgttaa aaactgaaaa
       61 caataacgca ttaaggaaat ttgacatttt taatagttcg attgaagttc cctatatcga
      121 aaaggtaagt gcagatgtat taaaagttga agaaattact tatgaaaata ttctaaaaga
      181 cataaatttt gatgtcaaag ttggagatgt tattggtttg gtagggaaaa acggtgtggg
      241 gaaaacaacc ttactaagaa tactcatgaa gattattcgc cctaccaaag gaaagataat
      301 cgaa
//
LOCUS       DAZUKT010000261          292 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000261 DAZUKT010000000
VERSION     DAZUKT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.282"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACG816_003176"
     CDS             <1..185
                     /locus_tag="ACG816_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4921955.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAZUKT010000262          281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000262 DAZUKT010000000
VERSION     DAZUKT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.283"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>281
                     /locus_tag="ACG816_003177"
     CDS             28..>281
                     /locus_tag="ACG816_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4921956.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           84 a           53 c           56 g           88 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata c
//
LOCUS       DAZUKT010000263          269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000263 DAZUKT010000000
VERSION     DAZUKT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.284"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>269
                     /locus_tag="ACG816_003178"
     CDS             <1..>269
                     /locus_tag="ACG816_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4921957.1"
                     /translation="VITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSI
                     TFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           93 a           54 c           48 g           74 t
ORIGIN      
        1 gtcatcacac tcgttgagcg actttcaaaa gccattatcg ccttgaaacc agaaggccgt
       61 aaggcagttg atattgaaaa ttcgattaat gaatggcttc aatccgtacc caaaaatctt
      121 ttcaaatcaa ttacctttga ttgtggaaaa gaattctcta attggaaaag tattagtaat
      181 acgaatgata ttgatattta tttcgcagac ccaggaacgc cttcccaacg gggattaaat
      241 gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZUKT010000264          264 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000264 DAZUKT010000000
VERSION     DAZUKT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.285"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           98 a           43 c           49 g           74 t
ORIGIN      
        1 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
       61 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      121 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      181 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      241 ttttgataaa aaacagtggg ttga
//
LOCUS       DAZUKT010000265          261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000265 DAZUKT010000000
VERSION     DAZUKT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.286"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>261
                     /locus_tag="ACG816_003179"
     CDS             <1..>261
                     /locus_tag="ACG816_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921958.1"
                     /translation="PGQAATITIGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPG
                     INATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNATTTT"
BASE COUNT           87 a           54 c           56 g           64 t
ORIGIN      
        1 tcctggccaa gcagcaacaa ttaccatagg aaccgtttct agtggttcaa ccgcttctgt
       61 tacgaatgta ggaacatcat ctgcagctag atttaatttt gttttaccaa agggagataa
      121 aggtgatcct ggaataaatg ccacaactac agctgtagct acaactacag caaatgggtt
      181 gatgtcgtcc acagataaaa ctaagttgga tggaattgca gctggagcac agaaaaatcc
      241 aggtaatgct acaactacga c
//
LOCUS       DAZUKT010000266          261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000266 DAZUKT010000000
VERSION     DAZUKT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.287"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..>261
                     /locus_tag="ACG816_003180"
     CDS             101..>261
                     /locus_tag="ACG816_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4921959.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
BASE COUNT          100 a           48 c           39 g           74 t
ORIGIN      
        1 tttattacgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
       61 aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata cccatcttac
      121 aacggatgaa cttgtaataa tagagtctta tttcaaaatg aatcaatctg ttgctaaaac
      181 tgcccattgc ttgaatcgtt caagacaaac gatccataaa gtatatctgt tcttcaagca
      241 aggaaaatca gccttagaat a
//
LOCUS       DAZUKT010000267          255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000267 DAZUKT010000000
VERSION     DAZUKT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.289"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          102 a           38 c           44 g           71 t
ORIGIN      
        1 aaagagttta aagcgtaata cacttatggg gaagtggttt ggggtgcgct ttaaactctt
       61 ctttattatt atctcacagc atgcttaaaa ggtctatgga ttaagttatg taaaaaagtg
      121 cctctttaaa cgtcctaaaa tccaaacaag gatagcataa tgtacactat cgttgatcca
      181 gaaaacaggt aaataaattt tgcaaaagca ataagttaaa aacattacaa aaaagacaag
      241 gatatcacat caaga
//
LOCUS       DAZUKT010000268          253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000268 DAZUKT010000000
VERSION     DAZUKT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.290"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACG816_003181"
     CDS             <1..>253
                     /locus_tag="ACG816_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4921960.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZUKT010000269          233 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000269 DAZUKT010000000
VERSION     DAZUKT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.291"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZUKT010000270          224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000270 DAZUKT010000000
VERSION     DAZUKT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.292"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG816_003182"
     CDS             <1..>224
                     /locus_tag="ACG816_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4921961.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZUKT010000271          223 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000271 DAZUKT010000000
VERSION     DAZUKT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.denovo.293"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>223
                     /locus_tag="ACG816_003183"
     CDS             <1..>223
                     /locus_tag="ACG816_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4921962.1"
                     /translation="KLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQ"
BASE COUNT           70 a           45 c           44 g           64 t
ORIGIN      
        1 aaattggatg actcttcttc ctattgtgaa cactgtcgat cggttcgtat tatccgcaac
       61 ggttcctata cgacacgcac tcagatgttg aaagtcaaag aaaagttaac tattttagaa
      121 ttaaaacgta cccgttttct ctgccatgat tgtggaagga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc agg
//
LOCUS       DAZUKT010000272        21506 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.051, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000272 DAZUKT010000000
VERSION     DAZUKT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.051"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACG816_003184"
     CDS             complement(<1..353)
                     /locus_tag="ACG816_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4921963.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWT"
     gene            complement(397..2430)
                     /locus_tag="ACG816_003185"
     CDS             complement(397..2430)
                     /locus_tag="ACG816_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921964.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(2464..3315)
                     /gene="rfbD"
                     /locus_tag="ACG816_003186"
     CDS             complement(2464..3315)
                     /gene="rfbD"
                     /locus_tag="ACG816_003186"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4921965.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(3413..4441)
                     /gene="rfbB"
                     /locus_tag="ACG816_003187"
     CDS             complement(3413..4441)
                     /gene="rfbB"
                     /locus_tag="ACG816_003187"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4921966.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(4463..5035)
                     /gene="rfbC"
                     /locus_tag="ACG816_003188"
     CDS             complement(4463..5035)
                     /gene="rfbC"
                     /locus_tag="ACG816_003188"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4921967.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(5049..5915)
                     /gene="rfbA"
                     /locus_tag="ACG816_003189"
     CDS             complement(5049..5915)
                     /gene="rfbA"
                     /locus_tag="ACG816_003189"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4921968.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(6015..6722)
                     /locus_tag="ACG816_003190"
     CDS             complement(6015..6722)
                     /locus_tag="ACG816_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4921969.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(6727..7554)
                     /locus_tag="ACG816_003191"
     CDS             complement(6727..7554)
                     /locus_tag="ACG816_003191"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4921970.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(7554..8354)
                     /locus_tag="ACG816_003192"
     CDS             complement(7554..8354)
                     /locus_tag="ACG816_003192"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4921971.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(8347..9486)
                     /locus_tag="ACG816_003193"
     CDS             complement(8347..9486)
                     /locus_tag="ACG816_003193"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4921972.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(9691..10884)
                     /locus_tag="ACG816_003194"
     CDS             complement(9691..10884)
                     /locus_tag="ACG816_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4921973.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(10963..11169)
                     /locus_tag="ACG816_003195"
     CDS             complement(10963..11169)
                     /locus_tag="ACG816_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4921974.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(11162..12304)
                     /locus_tag="ACG816_003196"
     CDS             complement(11162..12304)
                     /locus_tag="ACG816_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4921975.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            12816..13451
                     /locus_tag="ACG816_003197"
     CDS             12816..13451
                     /locus_tag="ACG816_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4921976.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            13587..13679
                     /locus_tag="ACG816_003198"
                     /pseudo
     CDS             13587..13679
                     /locus_tag="ACG816_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            13793..14428
                     /locus_tag="ACG816_003199"
     CDS             13793..14428
                     /locus_tag="ACG816_003199"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4921977.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            14535..15773
                     /gene="mef(H)"
                     /locus_tag="ACG816_003200"
                     /gene_synonym="mefH"
     CDS             14535..15773
                     /gene="mef(H)"
                     /locus_tag="ACG816_003200"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS4921978.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            16000..16419
                     /locus_tag="ACG816_003201"
     CDS             16000..16419
                     /locus_tag="ACG816_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4921979.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            16424..16663
                     /locus_tag="ACG816_003202"
     CDS             16424..16663
                     /locus_tag="ACG816_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4921980.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(16888..17811)
                     /locus_tag="ACG816_003203"
     CDS             complement(16888..17811)
                     /locus_tag="ACG816_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4921981.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(17843..18607)
                     /gene="map"
                     /locus_tag="ACG816_003204"
     CDS             complement(17843..18607)
                     /gene="map"
                     /locus_tag="ACG816_003204"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4921982.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            18765..19205
                     /locus_tag="ACG816_003205"
     CDS             18765..19205
                     /locus_tag="ACG816_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4921983.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(19383..19952)
                     /locus_tag="ACG816_003206"
     CDS             complement(19383..19952)
                     /locus_tag="ACG816_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4921984.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            20207..21439
                     /locus_tag="ACG816_003207"
     CDS             20207..21439
                     /locus_tag="ACG816_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4921985.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         6598 a         4233 c         3843 g         6832 t
ORIGIN      
        1 tttgtccact cgatccattt aattactaaa caaaataaaa taatcaaagg aaacagtaag
       61 tctccaaata aagactcatt tcttaattcc cgaaagtaag aaaagaaaat gaaaagggca
      121 agcgcaacac ctagataaag aaaatcgacg ccttttgctt tataaacatt taaaatgagc
      181 aaaatagcca cactaaaagc gataagtaat gaactattgt tatatataaa caaagttgct
      241 ggaaaaccta accctgctgt ccaaacaaaa aagactagcg tcagtaacaa aagcaagaaa
      301 ccgacactca ttttgttgac aaatttttgt acgattgctt gaattatctc cactctcctt
      361 tctactaaca tttcaggatt attctcagct agcctattac tgtacgacta gctgattttc
      421 ctgagtttgt gtttgtggta catctggata atccggatat tctactttaa aaatcatata
      481 accgtcttta tccggtccat atagctgcgt gaactgcgct tcatataatt ggttgtaatc
      541 agttaatggt cgtttactga tcacatagtc gatccctaat ttttctgcgt cttcaaaatt
      601 caacgtgacc gaaaacatat ctggacgatc taaaatgaac tttgtatctt ccttagcgat
      661 aaaagctctt gtatgagcat accgattata atacttctcg tattcttctt tcggatcgat
      721 tttcttccat gcagctatat ctggataaaa gcgtactgta ttgaaggatt tcactcccaa
      781 tgttggggtg aagttatata attcatcttc agacaaccaa actttaccag gatcttgttg
      841 atcgattttt tggatttctt ttgccagtgt tttttcaaaa atcgctcctg taccttctac
      901 gactggattg acgaaaaagc cagatacgat aactaaggcg gcaaaagcga ggtaaaacaa
      961 gcgtctccag ccaaataatg caaaagctaa aatcactgtt cccaacacag caactgcgat
     1021 ggtttcaaaa tccgaaaaat aattatccat tttcgaatgc gtaatcgcat agaaataaga
     1081 ccatgcattt actgccaaca aaatgaattt ataccaaata ggaataacat cttctctcca
     1141 aatataagcg ataaaccaga tcgataataa acaagccgca aaactaaaag taaggatggc
     1201 tcttaatgga ggaacataag acagcagagt gatttctgct agccattttg gcaacccaac
     1261 ggtaatccaa aagatagaaa acagacaaaa aatcatcaaa atacgtccca gtaacttttg
     1321 ttcgctatct tttttgccaa ataacacaaa tggacttgct aggaatacag ttggaaaaaa
     1381 gtgatagaac agggccactt cagagttatt cgaatagttg atatcttcaa aaggaatttt
     1441 ccaatttgta aggaaagaaa aaaattcgcc aatcgtccac tttcctccag tacttacccg
     1501 ttttcctgga tataaggtat tcaaagaagc taacaatgcg tcttttgacg ttagccagaa
     1561 atgaaccatg actccgccaa taaataaaac tgcccctcca atcaacacta catccaacag
     1621 atcccatgaa actttcttgc tatagtaaat aagcaggcca acaaaataaa aaatcagcaa
     1681 gtacgccagc atcacttgat gggctgggta aatcactaaa ataaacccaa taccgaaaat
     1741 aaccaccaaa agcatcatca acgttcgtag ccatttttta tcatgattgt agaaataatg
     1801 ataaaaagca gtcatcagtc ccaaagtaaa gaaaatcaaa tcgcccacat gttgcatgaa
     1861 ccaccactgg actgctggag aaaatgtcag cgcaaatgca ccgattagcg atagagcttt
     1921 ttttctcttc gttaatatca tcagtacttc gaatccaagc aagaccatga cgactaattt
     1981 catcgcccaa taaaaggaaa gtcctcgatc tcttccgagg aaaaagaatc cccagttgaa
     2041 tggttttccg ataaccgtaa tatcctttac aggagaatta tacgcaatga tcatgttttg
     2101 tccgtttaat gaataatcct cgttcaccaa cggaaagtcc tcttcagctt gtgagaggta
     2161 aaaaggtgtc tgcaccagcc attcgtctga ccgcacttcc cgattctgtc caaaaatcac
     2221 atcagttttt ttcccatcgt ctctttgcga cacatattta tcccaaacac caagtgaact
     2281 tccgtttaaa ttaaaagtaa cgatgaccac gatgactaaa acgccaataa gatagcggag
     2341 ttttagaaac aggtcaagca ccaaattcga atctgcctgg ttcgcttttg gcttttcttt
     2401 tcgtcgtgca cttcttgttt gtttttccat atcatgctcc aaattctatt tgataaaagg
     2461 aaattactta ttcatttttt tggcagcttc cagcatttgc gcaagtgctt cttgccaagt
     2521 tggtatgaca aatcctaact cttctgtctt tttcaggctc atgactgaat actgcggacg
     2581 tgttgctttt tgcggaaatt cttctgacgt aacaggtgca acttctacat ctgtatcttt
     2641 caatatttct tttgcgaatt cataccaaga acagctattt tcgttcgaca aatgataaat
     2701 accaaatggt gctttttctt cgatgacata taccataaat tctgccaaag tacgtgtcca
     2761 agttggtcgc ccaaactggt catttactac agtcagtttg tcgcgggttt ccgctaggcg
     2821 ttgcatcgtg aagacgaaat tgtgtccata gatcccgaaa acccaagaag ttcgtatgat
     2881 ataatagtct tctaaaatct cttctactgc tttttcgcct aataatttcg tacgaccata
     2941 ttcatttagt ggattcgttt ggtcgtctac ttcgtattct acatttttgt tttttccatc
     3001 gaaaacatag tctgtactga tatagacgaa ttgagcattc acagcttttg ctgcaagtgc
     3061 tacatttctt gtaccatcaa cattgatctt ctcatctaat tcttttgctt cttcttctgc
     3121 tttatctact gctgtgtaag ctgcacaatg gaaaataaca tcaggtttta attctgtgat
     3181 gtaagccatt gttttctcgg catctgtgat atccaagtct tttgcatctg ttgaaacata
     3241 ttgcagattt ttttcatcta ataaatggcg caattctgta cctagttgcc catttcctcc
     3301 agttaataaa aacatatgat tatccgctcc ttgattttta ttttaaaaaa gagacaggcg
     3361 agcgcctgcc tcacatgtgt ttttccatta atacagtgac attttctctg tattattgtc
     3421 catttttggc atatgctgct tcaacggcct ctttttcttt tttccaccag tcttcatgtt
     3481 cttcgtacca tttgatcgtg tcagccaacc catcacggaa gttcgtaaac tcaggcttcc
     3541 atcctaactc ttcccgtaat tttgtcgaat cgattgcata acgcaagtcg tgtcctgcac
     3601 ggtcgtttac atggtcgtac gcatcttttg gttgacccat catttctaag atcaattcca
     3661 tcacggtctt attatcttct tcaccgtctg caccgatcag ataggtttcg ccaattctgc
     3721 ctttcgtcaa aatcagccac actgctgaag aatgatcatt cgtatggatc cagtcacgaa
     3781 cattctttcc ttcgccatat aattttggac gaataccgct taagatattc gttacttggc
     3841 gaggaataaa tttttcaata tgctgataag gaccatagtt gtttgaacag tttgaaatgg
     3901 ttgcacgtaa gccaaaagaa cgcacccatg cttttactaa cagatctgaa ccagcttttg
     3961 tagaagaata tggacttgaa ggattatatg gtgtttccgc agtgaatttt tctccttcgc
     4021 cttccccatg tcctggcagg tcttcacgta atggaagatc gccgtaaacc tcgtcagtag
     4081 atacgtgatg atagcgtaca tcatacttgc ggcaagcttc taaaagtgta tacgtaccaa
     4141 tgatattcgt ttgtacaaat gggaaaggat cttttaatga attatcgtta tgggattctg
     4201 ccgcgtaatg aacaactgcg tccgcttctt ttactagacg gtccactaat tctgcatctg
     4261 caacatctcc tacgaccaat tctacgcgat cgctaggaag tccagccaag ttctctttgt
     4321 ttcctgcata agtcagttta tccaaaacag tgacatgcac ttctggatga ttgtttacca
     4381 cgtaatgaac gaaattagag ccgatgaatc cggcacctcc ggtaacaatg atgtttttca
     4441 taagaaaatt tctccttaat tcttagattt ctccgtaaac aaatggattt tctgcttcaa
     4501 attcttttaa tgtcggatga cggtgatctt tttccgacat aatcgctctt gatggatcaa
     4561 ttggccaatc aatcgctaaa tctggatcat caaatgcgat gcccccatct gcttctgcat
     4621 tatagtaatt atcacactta taaaggaagt tcacatttgg tgtcaaagta acgaatccat
     4681 gggcatatcc tttaggtaca agcaactgtc gatgattatg ttcagataag ataaatccgt
     4741 cccattgacc ataagtcggg ctacctttgc ggatatccac tactacatct aaaactgctc
     4801 cagtgactac ccgaatcaat tttgtctgtg cagcttctcc tttttggaaa tgaagccccc
     4861 gaaggacacc agcttcagct gatagtgaat ggttgtcttg aacaaagtca tatttcaatc
     4921 catgttctgc aaattttgct tttgaataac tttctgtgaa gaaaccgcga tgatcgccaa
     4981 agacgtccat ttctacgatt ttgacatctg ttaattttgt atctgttact ttcatctgta
     5041 aggactcctt attcagcggc caaattcaat aaatattgac cgtattgatt ttttttcaat
     5101 ggctgtgcaa gggccagcaa ttgttcctta tcgatgtagc ccatacggta agcaatttct
     5161 tccaaacaag ccacttttaa gttctgacgt ttttcaattg tttcgataaa ggtagatgct
     5221 tcaagcaatg attcatgtgt tcctgtatca agccaagcaa atccgcgtcc cataagttct
     5281 acggatagtt ttcctttatc caaatatgct ttattgacat ccgtaatctc tagttctcca
     5341 cgttctgaag gtttgatacc tttagcgata gaaattacct cattgtcata aaaataaagg
     5401 ccagttacag cgtaatttga ttttggctga gcaggctttt cttcaatcga caaagcttgc
     5461 atattctcat caaattctac tacaccaaag cgttctggat cattgacatg atagccaaat
     5521 actgtcgcgc ctgtatcttt tgaagctgct cgttgcaaca ttttagacag accgccacca
     5581 tagtaaatat tgtcccctaa taccaagcag acactatcat ctccaataaa ttcttctcca
     5641 atgataaacg cttgtgccaa tccatctgga ctttcttgta ctgcatattc gatctgcagt
     5701 cctaaatcat ttccgtttcc aaataattcc ttgaaacgtg gtgtatcttg tggcgttgaa
     5761 ataatcagaa tttctttgat tcccgccaac atcagagtag acattggata ataaatcatt
     5821 ggtttgtcat aaatcggcat caattgtttc gatgttgcct ttgttaaagg gtacagacgt
     5881 gttccgctgc ccccagcaag aatgattccc ttcatggata tctgctctcc tttttgattt
     5941 caaatttcac tttgttatta tctcatcaat tgttatattt gtcatggaaa tattaaggat
     6001 ttaaacatct tttatcaacg tttaaaccat tttcgtttga tcaacgccgt aatcagatga
     6061 acacgccaag aaattttttg ccgtaattta gaatgtgttt caatctcact cgcgtttaaa
     6121 tcatgtcttc ggtacagagt taacggttct cttaggaacc cagtcttctt tcctcccagc
     6181 aaaccaatcc acatatcatg catcggcact tctgacggga tcggcaaaat atcgggtttt
     6241 aaagaagacc ggaaagccat accagcacca atatagtgac ttcttatcac attattaagc
     6301 catccttctt tggaatttcg aaacgaaaaa tacgaatcat ggatctcttg cagctcgctg
     6361 tccacaatga ttaaatcaga aactactact agctgatccg gattctgctc aaaatagtca
     6421 gtgatctttt tcacttttgt ctccaaccaa acatcatctt ggtctgccaa aaagattaga
     6481 tctcctttcg tctgctggat cgcataacca aaattagcga tcaatccttg acgaggcccc
     6541 gaaaaaagct ggattcgctt atctttttct gcgtattcag ttaatatatc ccatgtatca
     6601 tctactgacc catcatcaga aatgactact tcatcttctg ctgaaagctg agataaaata
     6661 ctatctaact gctctcttaa atatctgcct ccattaaagg tagctataca aaccgaacgc
     6721 acgaattcac accttccaat tcttaacata tgagcgcaaa gtcatccgcc agatatgccg
     6781 gtttttgacg gtcaagaact gtttagctgt tcttaacaat aattgtcgtc ggtgactgga
     6841 taaatgcctt tgatcatatt catgataaaa tcgattttct gcttgtaaaa tagaacggta
     6901 tcgattactt gaaacttttt catagtctaa aaccgaaaga tcatgttcca ttcgtacagg
     6961 aaggatctcc acagtttttt ccaactgatc cactttccaa aacagccaat gatccaaaaa
     7021 atctaaagaa aaagcaggat taaacccgcc aatctttttt agaaatttga cagaccaggc
     7081 aacacctgaa ttgattgcca tgattcgttc agctgttggg ccttcaactt ctactgcttg
     7141 agctgtacga ttgatataac gatctgaata taggggagaa acttgtcggc tcccagaaaa
     7201 aatcatcgga actgcagcaa cacattcatc tgtaaaggtt actttacgca atagttcaaa
     7261 ataatcgtcg actagcctcg tatcctgatc cagagtgacg agacaacgtg ttccttcatc
     7321 tgcttgagac aacgcaaaat tataagccac cgctaaacca ggattttgcg gatcatgatg
     7381 ataaacgata ttttcttttt caaacagctc attttgctgc ctttgaggac tattatcgta
     7441 aataataagc tgaatttgct tttcgctaag tgctttgctt aatggtccat aagctggaga
     7501 ttgactgaat agttgctgat aaagcacaac gactactaat atttctggtt tcatcagaaa
     7561 aaacgccacc cccacttatt gaaaaatttc atcatagaag tcaaaaacac gcgaaacagt
     7621 tttgcatttc gatgcgcacc cttcccgtaa aaatggataa ctgcataaaa gggtgtgtac
     7681 aataacttgt aacctgcttt ttcaaaactc aaacacaagt cgttgtcttc aaagtacatg
     7741 aagaaacgct catcaaaccc ctttatctct ttaaatttat caatatcagc taccataaaa
     7801 ctacctgaga tcatccgtac ataactatta gcatcagtag gcaatcctcg gcattcaaac
     7861 acagccagtc gtttgtcaaa cttttttttg acccatttga aaggcacaaa gcgcaaaacg
     7921 taatcaaaaa cagccagctg ctcacggatg aggtgctgag ttgttccatc ttcattaaga
     7981 atcttaggtg ctaacatcgc acattccgga tgttctttca ataacaagac caatgctgta
     8041 actgtctctt catccactaa aatatctggg ttacaaataa tgccatagcg agaatcgcta
     8101 gctatcagat tgtgattatg tccataacca aatccggaat tttcatcatg aaaatagata
     8161 tcgatgtagg gatatttcct atactctgtt agtctttttt tgtactcggg aagtgaattg
     8221 ttgtcaaaaa ttcggatttt tactgctgtt tcatctttta cagttgaaat aaaattatct
     8281 agtgtccgaa aaattttatc actgtttgcc gtcacaatac taataaggat cggctggttt
     8341 aatttgttat tcatctatat gacggttcca tttcttcaaa tatttctgtt ttacttgcgt
     8401 acggtactct ttattcccta aaaatcgcaa tgtttttaaa ataaactgat gtttctcatt
     8461 gatcagacca atcaattcaa ataataaaat tgcagcaaac aacatggcaa taatcaatat
     8521 gatcgttccg attcttccag tataattcat gaccatagct gtgaaagaaa acatcaaagc
     8581 catcgcataa atcgtcagga cagcaccttt atgagtaaag cctaaactga ttagtcggtg
     8641 atgaagatgt tttttatctg ctgtagaaaa agaaacacgg tttgcttttc tcctaatgat
     8701 agcaaaaaac gtgtctgtta ccggcactcc catgacgatc agcaaagaaa tcgatgaaac
     8761 aaaagtcacg ttcttcagcc cttgtagtga aaggacggcc atcataaacc ctaggaatag
     8821 agctcctgta tcgcctaaat aaatctttgc cggataaaaa ttgtaaggaa aaaaaccgat
     8881 aatacttgct acaagacaga aaataacaac tggaatatac actgtagaag catggaggaa
     8941 aaaatatcca atgattccta tcgtaattaa accgatcaat gaaatccctg ccgccagccc
     9001 atccaaaccg tcaatcagat tgaccgcatt cgtgataccg aagatccaaa aaatcgttac
     9061 gggaaaacta agccagccta gttggatata tccaataaaa ggaagcgtga aggaatcaat
     9121 acgtatatct gctacaaaat aaataataag agaagcaatc aagatcccta tacttttttg
     9181 tttaggtgtc agctcatatt tatcatcgag cactcctgtg actatgatta ctaaagaagc
     9241 aatcaagatc ggtacaatat acgattgagg gataatttta cggaataaga ataaagtcga
     9301 tataacaaat gaaataaaga taggcagtcc gcccgctgaa ggcattggaa ctttatttat
     9361 tcttctttca tttggtttgt cgaccatacc tgtttgtact gaaatgattt tgaatacagg
     9421 tgttaatatc agtgataata aaattgtcag aaaaaatctt ataaccatac taaaagaaac
     9481 aagcatatgg gcacctactt taattaagag tcataattat tataaattat atatgtcttt
     9541 ttgactatct ttttatatct tctcaaatgt ccgcaaagcc ttattttatc ggctctacgg
     9601 gcattttgct tttgtggtaa acctcgcata tctaggtcta tcttcttata ttttccctgt
     9661 caatcgtggt taaaatcgtg gtaaatactt ctatgcgatt agacgctgaa cctctgattt
     9721 tgcggtatct atggacgcat gagcgtacca gttcattgtg atactgatgt ttgaatgccc
     9781 catgatatac tgtaaatctt tcgggttcat gttcttgctt gccagccttg tgcagaatgt
     9841 atggcgtagc gtatgcggtg taatatgtgg caagggattg tccttgtggt gcttgttgta
     9901 tttctttacc atacggataa ataaggcgtt ataatcaatg gcaacttttg gattaccttt
     9961 cggattgata aacacaaaac cgctgtgtcc gtctacctct atcgacttta tctttggtcg
    10021 gctgttgata acccgttgaa atgcctgtat cgtttctttg cttaatggca cttgccttat
    10081 cccgcttttc gtcttaggcg tttcaatata atagccctgt tccttactct ttagtaactg
    10141 gtggtcgata atcacaactt cattcttgaa atcaatgtcg gctactgtca gtccgcacag
    10201 ttccgagata cgaagtcctg tctttaacag tatcagcaca tcatcataat gcttgtgata
    10261 cacgttgtcc gtcttaataa atgacaataa ggcttgctcc tgttcctctg tcaatgcgac
    10321 tttctctttg gtatcatttt ctatgacttc acttagcttg aaatcaaaag ggtttttcct
    10381 cacataatca tcttgtatgg ctatgtagaa tgacgctttt aacgagcgtt tatggttgtt
    10441 aatggtattg taggaaaagc ctttgtcttt catgcgtaac gcccattctt tagcgtcaga
    10501 gggttttatc gtatcaatgc tcctagcacc taacttgtcc tcttttaata accgcatgag
    10561 ttgttcccgt tgtttctgtg tgcttttctt cacatttgtc ctctgtgcgt tctgtttggc
    10621 atagagttgg caaagcgtca ttttcttgcc tgtgctgtcg ataccgtcct caatatcccg
    10681 tcttatctgc tgttccagtt ctcgaagtga gggtttttcc cgttttccct ttggtgtcgg
    10741 gtctgtgggt gtcaatctcc aagcatacac atattttgtg tttcccaatg cgtctacata
    10801 tttatataag tattttccgt ctgttcgttg gctctctcca gtatgcagga tacgaccttt
    10861 gttatcccgt cttttttctt tcatggtgtc tgctcctttc cttgttggaa agagccttga
    10921 tatgacttgt gttcatcata acacatacaa ggctcatttg cattagattg cgtccaattt
    10981 atcaataacc ttttcaaact gtcggcgttt aatctgtatg cggttgccat tcataatgag
    11041 ccagccagcg tccttgtttt cctctgccaa tcgtcttaac ttgttttctc caatgcggaa
    11101 atactttgac gcttcttcaa tggtaagggt gtatttttcc catacgggaa tatcctgtgt
    11161 attactcata aaacaccccc tcactttcga ttacgtctga tatgtccgta cacatctgtt
    11221 ccacaagcat ttcctgtttt tcagaaaggt gggtacattc atcaatcatt ttcatgtagt
    11281 ttgaacctgt cagcctgtcg atttcctcta tcatcacgat tgacggcatg acctgtcttt
    11341 ccagccattg cagagtgcgt tccatattga caggtacagg gttcatttca aaaggtaaac
    11401 tgtcatggga aatgaatatc tcccacaatg gatagtcagg aaattccaca aaatcggtaa
    11461 tgagataaaa caccagccca tgagggttat acgtcaataa cagttcttca actgcctgta
    11521 ccgccttttt gtcacgcaga cgaatttcaa aacggttgat aatatcggta tgtttctttt
    11581 tcgttgcctg ttccttttgt ttttcataca ggcagaaata ttttgtgctt gcctttgagc
    11641 cgatataaag ggtacttgct aaattacggt tttttccgct taattttcca ccttgtacat
    11701 tttcatagtt tttacagcga cattccgcac caccatttct gtatttttca gatagcacag
    11761 gaatatccag cagaccgcac atatcattga ttgccaagtc aaaacgtcgg ataacaccgc
    11821 cacagtcaag acagcggttt aagaatgaat accagtccct gttctgtgcc tgtaagacat
    11881 attccatgtt gcgtgagcct gcccctttta attctaaaaa tacgcccatg tgttcatcat
    11941 cagaagccat gaccttgatt tccccgtaag cataatgctc cttatagcca taatacccat
    12001 agtcataatg gataaaataa tctgctttca tgccaagcac ctttcggata acttccaatg
    12061 cgtcagtcgt tggaaaacgg acagacagat aatcaacgag cagataaaaa ggcttatcgc
    12121 agatatagta attcagacaa cgcaggataa tgttctgtgt ttccttgctg gcgttagctt
    12181 ttccgttctc aaagcggttg atactatatc ttgatacatg ggtaagactt gcaagttctc
    12241 cttgtgaaag accgctggca agacgtttgt tcttcatttc aagtgcaaat tctaaattgt
    12301 tcattgtatt cctccaatcc cacaatagcc ttgaaatacc tgtaaaccac ctttcaaaaa
    12361 gtgtgatatg tactatcttc ttaaaacctc tcattactac tcacaggttt gggggtgtat
    12421 ggtttttgac ttgtcttttg tgaaatagtg cccccctgtt agataacggg ggcacagaca
    12481 aggctcgcag gctcgccagc cgacaaagca tttcttcccc ttgacacaag gtgtcatggc
    12541 ggtaagcctg agaggcttcc gccaccgtgt cagaaatact ttgtcagcaa gttctgtttt
    12601 gccttttcta acaagttcca tttcaaccga tattcatatc tacggattta atcagatagg
    12661 tatttctttt tgaatgtttt tatatttcat tctattgtgc ttattcgatt acaagaacat
    12721 agtaattctt atatgcttat ttgtcaatat ctttttttgc ctttacaaat catattaagc
    12781 atgatagact tataatagcg aagatataac gagatatgga gggttatgca atgaaaataa
    12841 gccatttagg aaataatata cagaccataa gaaaatttag gggaatgaaa caacaggaac
    12901 ttgcggacaa aatcggtatc aatatgcaga gcctttccaa gattgaaaga gggttgaact
    12961 atcctgccta tgaaacgctg gaaaagataa tggaagtgct ggacgttacg cccaatgaat
    13021 tgttgtcggg agaatggaag tatgtcaatc aatctgaaaa ggaagtctgt cagtttttaa
    13081 gagccgaaga acgtctaaat gcagaactga aacatggaca ctatgataac ttctttgaca
    13141 gcgaggaaga atggctggaa tatgagttag aaaagttacg ggaatatatt acagactata
    13201 tcaacgggaa aagcattgaa gcgtccgacc tttacccaat caaagaattt atccagcatt
    13261 tgaagtttca aaaattatta gaccgctatg atgatttata cagtatggat atgtttgggg
    13321 aaagcataga gggacataag tataagacac cttatcaagt cgtgaaaatg ataaacccga
    13381 actcaaaaga agatatggaa ctgttacgag aagtattaag gaataacaat tttgatgatg
    13441 aggacgagta acttttttcc tcttgcgaga agatacagca aacaaaaagc ccgatcaaga
    13501 gtgcaaagca ccaccaatgg tggcaaagct cttgacaggg ctttttcttt tctgttgtgg
    13561 gcaatcaaga ggaagaaagg gataaaatct gatttgtggt aaatcaggca aaggcaacgc
    13621 aggtatcaca gtatgaattg acaccgcata aggaaagcaa ctggaagatt gtagaataaa
    13681 tgaaaaaggc ttgcattctg catttttata aaattgcaag tctttatatt gacaagcata
    13741 aaatactaag atatacttta atcagaccaa cggtttgtat tggaggtaag ttatggcacg
    13801 aaataaatat ccagaagaaa cagtaaaact cattttagat gtttctacaa aaatgttttg
    13861 tgaaaaagga tatgataaca catcattgca agatattatc aatgaaacaa aactttctaa
    13921 aggtgctatt tatcatcatt tcgattcaaa ggaagatatt ttaaaagcaa tttttcaaag
    13981 aattggaaat gaaaatgcag taatatttgc taaaattaga gataacgata gtttaaacgg
    14041 tttagaaaag ttaagagaaa tatttaagac agcggtattc aattctaacc aaagcgtttt
    14101 attgacagtt tctcctcaac tattaaacaa tcctcgtttt cttgctatgc aaatagaaca
    14161 aatgtatgaa attgtagtac ctgaatttgt tgaacccgtt ttagaacaag gtatcaagga
    14221 taagtctttg gagattgaaa attcacatga aattgcagaa gttatcatgg tattaagtaa
    14281 tgtatggctc aacccattag ttgaaatgac ggataaagag ggtatggaaa aacgttgcaa
    14341 tacattcaat gcccttttaa atggaatagg catagacaca ttattagacg aagaaacaat
    14401 ttctgcattt atacagtttt gtaaataact gtatttgtta tccctatacg atagactgct
    14461 ctataaaaga gcagtttatt taaaccatta tataaaccga cggtctgttt gattaaataa
    14521 aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa tcgtgatttt atacttgttg
    14581 ttataggaca gataatttct ttatttggaa acgctatcct acgatttgca ttaccgtact
    14641 atttgttaaa agaaactggt tcatcagctt tgtttggtat tgctacagcc tgctcttttt
    14701 tacctatggt tattttgtcg ctggtaggtg gtattattgc tgacaggaca aataaaagaa
    14761 acattatggt catactagat tttgtaacag caatcataat tttagtattt tatttgtcat
    14821 tgggaaaaat tcctatcata cctttgttta ttgttgcttt gatgttactt tatggtattt
    14881 ctggtgcata tcaaccagcg gtacaagcaa gtattcctct attggtatca tcagataaac
    14941 ttaccgtcgg taatgctata ataaatcaag taaatacttt agcaaacttt atcggaccag
    15001 ctataagcgg agtagttttt caattttatg gtatcaatcc cattttaatt gttagtatta
    15061 tatgttttac tttttcagcc attatggaaa tttttatcca tattccacac caaaaaagac
    15121 ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt aaaagatagt tatacctttt
    15181 tgaaaaagga gaaacccatt tttatatccg taatcttttt ggtttgcatt ttcaatttgg
    15241 ttttaagtgc tgttttaatt gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc
    15301 cagatacact atatggtttt tcacaatcag cattatcatt aggtggttta tgtggtggta
    15361 ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa cctttatacc ttgttactta
    15421 tttgttctgt ttctgtttta cttatgggaa tttcgctatt tttagaattg cctgcaatag
    15481 tttcctactg ggtagttaca gtgatgtgct ttgtggctat gggtgtatct actatgtttg
    15541 ttgttcaaat ttacactctt gttcagacac aaacaccacc gcaattagta ggaaagataa
    15601 tggctacgct ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg gtttacggta
    15661 ttttattcga tgtatttata caagaaacat gggttgttat gtttgtagct tctattgctt
    15721 caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa gttagagata taatacaagg
    15781 ggttaaatag tatgttaggc taaaaacaat atataaaaga aaatttgaaa tctgctaaaa
    15841 ggatagtgtt ataaaagaaa gagtaatatt gcagtattta aggctcgcac aaatatatgt
    15901 tttgtgtggg cttttttctt accatttttc tgtatttcta aagggtataa atggtattcc
    15961 gtttatatat ttcagcatta gcagaaaggg ggtgagatta tgaggacatc ttctttcgag
    16021 catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa
    16081 agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc
    16141 gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt
    16201 aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga
    16261 ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga
    16321 gaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga
    16381 ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa gaaatgagta aagattatgg
    16441 ctatccctct tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga
    16501 gattttgaag ttttatgacg gttatatttc taaattatgc ttgcgtcctt tttaccactc
    16561 tgaaagtggt aaaatcatta tgcaggttga tgaagaacta aaaggagaaa ttcatacaga
    16621 catgatgaaa gcaatattga aatttgaaat cggagtgaag taatcaactg tataaaaaaa
    16681 tcgggagata cacagtttcg tgctatctcc cttagtagtt ttacggtcat atcaaggctc
    16741 attcaatcgt ggtaaattcg tggtaagatg agtgtttttc tatacttcaa aatgcttgaa
    16801 tgtatagtgt ttatgcggat aattgaaaat tagatataaa atttcaaata aattactgat
    16861 ataaatttac aataagaact cttgtttcta ctttttttta tgagaaaatt tagttttcaa
    16921 tttattgaac cattttcggc ttggatctcg acgttttttg atttgatgac ccgtcacgta
    16981 ttcttgaaca actgcattga tgattccgcc caaaatgatg atcgtagcag caaaattaag
    17041 ccaaagcatg agtacaataa aacttcctat gatctgataa ccgctgatcc tcgttgcaaa
    17101 atacctagca tagatactaa acacttgtga taacagcatc cagccgacag tagcgaagat
    17161 cgtgcccgga atgatcgtct tcaacttcaa ctgagcatta gggacaatta aataaatcaa
    17221 gaacatgatg acaaataaag ctaccaatgt gattggccac ttcaaagttt ggaactgatc
    17281 gataaagttc acgggtattt gtacgattgg ctgaatagca tccaaaatca gctgtcccaa
    17341 cccaagaacg atagttaccc cactaatagc aatcatgaac aagacaatga caattagtga
    17401 gaagaaacgg acgatcaaaa aattttttct attttctacg ccatacgctt tattcatcgc
    17461 aatttgcaaa gcattgatac tttgacttgc agaccataaa gccgctaatg cagaaacgga
    17521 taacaagcct cctgaacttt gtgtcaaaag gtctttgatt gcaggaccga taaactggta
    17581 aacggtctca ggaatcactt ctcgtaaata ggtcagtaca gtctcttgat cgatgtggag
    17641 aaacggtaaa atattaccaa aagcaataat caatggaaat aaagacaaga gtaaataata
    17701 agcaacgacg acagaggtcg tactcatttc tgaatcagtg atgcgctttt gagtcgtctc
    17761 aataaagcgc atcagatttt cattttgttt tattttgttc caaactttca tgtacctctc
    17821 ccgtacctat tgactggata gatcaataag tgccttcttc tccttgtgat gtcaaaattc
    17881 ttggaccatc tttagtaata gccaatgtat gttcatattg gcaagaaagt ccgccgtctc
    17941 ttgtatacgc agtccagccg ttaggatcca ttttcatttt ccaagttccg gtattaacca
    18001 taggttcgat cgtgataacc atgccttctt tcaaccgcaa cccttttcca gcttctccgt
    18061 aatgaggaac tgctggttct tcatgaatcg taggaccaac gccatgacca ataaaatcac
    18121 gaacgatcgc tagattttcg ccttctacat aagtttggat agcatgaccg atgtcaccaa
    18181 tccgattccc tacttgtgct tgctcaatac cgatgtacaa agctttcttc gttacttcca
    18241 tcaatcggtc gatctcaggt gtagattctc caactacata ggcccaacaa gaatcagaaa
    18301 tgccgccttt caaatcgata cacatatcga cttttaccaa atcaccgttt ttcaaaggct
    18361 ttttacgagg gaatccatga caaatttcat catttatact gatacaagtc gcatattcat
    18421 acccttcaaa gccaatttgc gcagcaattc cgccatgact ctcgatatag tttctgacaa
    18481 atacctcaat atcccaactt gtgattcctg gttttataaa atcacgcaga tttttatgta
    18541 catctgctaa taaagctcca gattctgcca tcatttcaat ttcacgtgct gattttaaag
    18601 taatcatatt ttcgtctcct ttttacaaat ccctctcatt ttaacatagc ggcatacatt
    18661 tagccaaatg acattcccca ggtgtctcta ggttttattt tcttttcaat gatgttttgg
    18721 tataattcat aattgttaaa taattataaa cgaggaggca catcatggct ttagcaaaaa
    18781 tcgtatatgc tagcatgaca ggaaacactg aagaaatcgc cgatattgtt gcagaagcat
    18841 ttgaaaatct ggatatcgaa gtcgaaatca acgaatgtac ccaagtagat gcagatgaat
    18901 tcgaggaggc agatatttgc gtagtagcta cttatacata cgatgatggc gatctgcctg
    18961 atgagatcgt agacttttat gaagacttgc aagaattgga tctattggga aaaatttacg
    19021 gtgtctgcgg atctggtgat actttttacg atgaattctg caaatcggtg gatgatttcg
    19081 atgctgcttt tgcaaaaaca ggcgcatcaa aaggagccga aaatgttaaa gtggatctga
    19141 atgctgaaga agaagatatc gaaaatttag aagctttcgc taaagaacta gtcgcaaaaa
    19201 tttaatatac ttgcaataaa agagccatcc agatttattc aatctgtatg gctcttttat
    19261 tcattgcatt catggaagag ttcttcggga gttaaaaatg catacgctgt aagaaaaacg
    19321 aacttttcct tattttgaca catctcttgt tacctactga tttgtggaac ttttgagtat
    19381 attcaatcga tttcgattgg ttctttgaat ttcaagatag catctatact gtcttggatc
    19441 atcttttcaa tctgttcatc agttggatgg atgatgcttg aagacatcaa agctgcgatc
    19501 ccagtagcta tgatccatgt cccattatac aaagattcga tctgctcttc tgaaagattc
    19561 acataagctg gatcgcgttt cactgtattc acaaagtaag tataagagaa attctgcatc
    19621 tcttttcctc caccatattc ttctaagtac agtgaacgat acaacttctt ctctcttctt
    19681 gcaaaatgga tataattaaa agcaagatcg ataatcgtat tccctgtatg ctcaacagtg
    19741 aacacttcat attgtaaatg atcatagatc tgctcaaaaa gagcttgtct caagtcatcc
    19801 atatttttaa actcaagata aatcggttgc gtggaacaat tcattttgtt cgcgatgttt
    19861 ctcgcagtaa atcgtgtaaa tccatcggtg gcagctactt cataagcagc tgccaaaatc
    19921 tgttctctag taatcgtctt ttttcttgcc atatttttta atctccttaa tttttttata
    19981 acgcttccac cattgattaa tttcattgta acatgttttt tttgatatgc aaaattagtt
    20041 tttgattttc ttcacaagct gaaatagcaa gtagtaacat atatcatgaa acttaacctt
    20101 aaaaaaataa cctccttttt ttatttaaag cctcattgtt tgaatcataa ttaaatggta
    20161 tcatgctaat agttaccaga aaaaatggtg aggaggaatt tttattatgt tatctgattt
    20221 taatgaactt ctaaaaaaat atgcacgctt aattgcagaa actggcgtag ccacagaaaa
    20281 aggacacaca gttgttttac agatcagcgt ggaacaggca ccccttgctc gattgatcac
    20341 acaagaagct tataaactag gagccgctga ggttatcgtt caatggactg acgaccagat
    20401 ccaaagagaa tttcttttac atgctgcaac ggatcgtatt gagaatgtac cacaatctaa
    20461 aatcgatcaa gcggatgaat ggcttgaaaa aggagcaagc aggatcagcg ttgtttctgc
    20521 cgatcccgat gcatttgctg gtgtagatag ccatcgagta gcgtcttatc aatcagcgaa
    20581 tggcaaagca ctaatgaatt tacgcaaggc aactcaagca aataaagtca gttggactgt
    20641 cgtggctgcg gctggtaaac aatgggcagc aaaagtcttc ccagaactgc cagaagaaaa
    20701 acaagttgag gcattatggg atcagatttt caaaacgact cgtgtctatg aagaagaccc
    20761 tgtcttagca tggaagaagc atgatgaaaa attagcaaaa aaagcagaag aattgaatcg
    20821 agaacaattt tctgcccttc actatactgc tcccggtaca gatatcatta tcggattgcc
    20881 taaaaaccat ctttgggaag gcgctggaag ctataatgct cgtggagaaa aattcatggc
    20941 aaatatgccg acggaagaag tgttcacagc acctgacagt catcgagtag atggttacat
    21001 ttcaagtaca aagccactga gttatgctgg caccatcatc tcgggtatga aatttacttt
    21061 taaagatgga aaagttgttg atttttctgc cgaacaagga gaagatgtct tagcaaaact
    21121 gcttgataca gatgaaggtg cgcgtcgttt aggagaagtc gcacttgtac ctgacccttc
    21181 acctatttct caatctggca tcatcttttt caatacatta tttgatgaaa acgcatcgaa
    21241 tcacttagca ctaggttctg cttatgcctt cagtgtgaaa ggcggtacag aaatgtccga
    21301 tgaagaatta gcagaagctg gattgaatag aagtcaaacc cacgtagatt tcatggtagg
    21361 atctgacaag atggatatcg acggaatccg tgaagatggc tctacagtac cgattttccg
    21421 aaatggcgat tgggcttgag tatactttcg cacacatgca aaaaataatg gctctttgtc
    21481 aataaggact gatgagttgt gcaaaa
//
LOCUS       DAZUKT010000273        14171 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.071, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000273 DAZUKT010000000
VERSION     DAZUKT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.071"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..911
                     /locus_tag="ACG816_003208"
     CDS             60..911
                     /locus_tag="ACG816_003208"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4921986.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(1162..3120)
                     /gene="nagE"
                     /locus_tag="ACG816_003209"
     CDS             complement(1162..3120)
                     /gene="nagE"
                     /locus_tag="ACG816_003209"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4921987.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3117..3989)
                     /locus_tag="ACG816_003210"
     CDS             complement(3117..3989)
                     /locus_tag="ACG816_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4921988.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(4331..5203)
                     /locus_tag="ACG816_003211"
     CDS             complement(4331..5203)
                     /locus_tag="ACG816_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4921989.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            5331..5900
                     /locus_tag="ACG816_003212"
     CDS             5331..5900
                     /locus_tag="ACG816_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4921990.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5967..6704
                     /locus_tag="ACG816_003213"
     CDS             5967..6704
                     /locus_tag="ACG816_003213"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4921991.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            6935..8203
                     /locus_tag="ACG816_003214"
     CDS             6935..8203
                     /locus_tag="ACG816_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4921992.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            8690..9154
                     /locus_tag="ACG816_003215"
     CDS             8690..9154
                     /locus_tag="ACG816_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4921993.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            9138..9767
                     /locus_tag="ACG816_003216"
     CDS             9138..9767
                     /locus_tag="ACG816_003216"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4921994.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            10121..11599
                     /gene="msr(C)"
                     /locus_tag="ACG816_003217"
     CDS             10121..11599
                     /gene="msr(C)"
                     /locus_tag="ACG816_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4921995.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            11646..12092
                     /locus_tag="ACG816_003218"
     CDS             11646..12092
                     /locus_tag="ACG816_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4921996.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(12291..12806)
                     /locus_tag="ACG816_003219"
     CDS             complement(12291..12806)
                     /locus_tag="ACG816_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4921997.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            12923..14125
                     /locus_tag="ACG816_003220"
     CDS             12923..14125
                     /locus_tag="ACG816_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4921998.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4455 a         2560 c         2924 g         4232 t
ORIGIN      
        1 taatttataa atgtacagct gtaaatctta acaaataaaa agaataggga gtggcaaata
       61 tgcattattt agtaacagga gcaactggag gttttggtgg ttatgcgctg gaattcttaa
      121 aaaagatggt tccacaaaat gaaatctatg tactagtacg tagtgaagaa aaaggagaag
      181 cactgaaaga agctggattt aatatacgta ttggtgatta tgatgattta gactcaatga
      241 aagaagcgtt gaaaggaata gatcgattgc tttttgtctc tggcgttcca ggaaatcgtc
      301 aagccgaaca taagaatgta gtgaaggcag cacaagcagc tggggtatct tatatcgctt
      361 atacaagttt tgcagatgcc gatcactcga tcagtgtatt agcgccagat caccaattca
      421 ctgaaaaaat gattaaggat tcaggtattt cccatacatt tttacggaat aattggtatg
      481 tagagaatga attacctatc attgggcagg ctttaaaaac tggtcaattc gtttatgctg
      541 caggaaatgg aaaaacaggt tgggcattga aacgtgaata tgcagaagct gcagcaaaag
      601 tactagtaga aagtgactct ccagaaatct tagaattatc cggcaatcta actgactatg
      661 aagaattagc caaggcatta gagcgtgcga caggtaaaga gttagaaatt attgaagcaa
      721 gtgatgcagc gtttgtcgaa aatctaaaag aagccggatt ccctcaagaa gcagctgaca
      781 tgtttttagc gatccaacat gatattaaaa aggatcagtt agctatttcc tctgatgatt
      841 tagaaaaagt attgggaaaa ccattaactt ctcttgaagc agcattaaaa gaaatattgg
      901 atcaggaata atgtataaag aggttgtgaa aaagccgatt agttttgagt atgaagaaaa
      961 attttgtgaa aaagctgttc tgcatcaagt aataagaaga gtcaaataaa aaatagctct
     1021 tcatatgttt cgtttagctg gacttattac cgcggagttg gcaggagaac gccgttttta
     1081 ttcggaaaaa ggagctgaga caagattttt gtctcagctc ctgtttgttt atcgataaaa
     1141 aagtctgggg aagacgatgg ctcaggcttt gttggaaaca gagccgattt tatcatttgc
     1201 ttttacatca cgtgcgggtt cgatttctaa gctttggact ttgtcgccat ttgtgaatac
     1261 aacgaccata tccgtgtttt tgcctgcaga ttgaatagcg gcaagatcaa ctaaagcaat
     1321 caattgacct cttgcgactt tttgaccttc ttctacatat aaagtgaatg gttcgccttt
     1381 taagtcaaca gtgttgatgc ccatatgaag caagacttcg ataccagcat ctgtttggat
     1441 tccgacagca tgtttcgttg ggaaaatgtt tgtgatcgta ccagcgattg gagagaagat
     1501 ttctccattt tctggtaata cagcatagcc gtcacccatc attttttccg cgaatacatc
     1561 atctgaaact tcagtgattg gaatcagttt cccatttgcg atagcgtaaa tatcttgttc
     1621 gatcgtttca ccggcaaatg tttctgtttt aaccacggtg tttgtttgtg caggtcgtgc
     1681 tgcagcacca ccatgtagtt ttgccatctc atctgcaacg aattgtactt ctgtacctac
     1741 gatgacttgc gcattttttc cgtcgatgat cttcatacca ggtacgccag tggctttgat
     1801 tttattttga tcgatcaggc ctgtatcttt gacttgcaaa cgaaggcgtg tagtacagtt
     1861 atcgatcgaa gttacattgt cggctccgcc taatgcagcg tagattttgc ttgcttgtgc
     1921 cgcatattta ttgtctgaat tagtcgttgc agtatcgtca tctgaaagat ctgcttcctc
     1981 gcggccagga gtcatcagat ggaatttttt gatagcaaag ttaaagccaa agtagtaaat
     2041 cactgctgtt actaatcctt ggacgatcaa catcaatggc tgattcgcaa tcggattttt
     2101 caaagatagg aagtaatcga caaatccggc actaaaggca aatcctgctg tccaatggaa
     2161 gaatgcactc actgctaaag aaatcccagt gaaaattgca tgcaagacat ataatggcca
     2221 agcaacgaac ataaatgaaa attcaagtgg ttctgtcaca ccagtaaaga atgaagcaaa
     2281 agctgctgct agcatcaagg aagctgtttt tgctttgttt tctggacgtg catttctgta
     2341 aatagcatat gctcctgctg gaagcccgaa catcatgatt gggaaaaatc cagcttgata
     2401 cattcctgtt acgccttttg ttccttcaga agccaaaaat ttaccaatat cattgattcc
     2461 tgctacgtca aaccagaaca cggagttcat tgcttgatgc aatcctgtag ggatcagtaa
     2521 acggttgaag aagccataaa gacctgcacc accccagcct aaactcaaaa tgaatttagc
     2581 aaaagaaact aaagcagagt aaaccggagg ccatacgagc agtagcactg cactgatcag
     2641 caacatgaca aaggcagcca agattggcac tagacgcttg ccgctaaaga aagataaagc
     2701 catcggtaat tttgcttcgt ggaaacgatt ataaagagct gccgcaatca atccggcaat
     2761 aataccaata aatacgttac tgatctgagc aaaagatgga tcaactgatt ctggtttgat
     2821 attcaataaa gtagctactg aatcagtttt taaaacattt actggaactt caaatgcgac
     2881 caaacctgcc aaggcagcag cacctgattt gtccactgac atccctagtg ctaacccaac
     2941 ggcaaacagc aaaggtaatt gacctaaaac tgcgttaccg ccagcttgta aaaaatgagc
     3001 gacaatgtcg ttactataag atgcccacca gtttccgatc cctactagta aagcagcagc
     3061 aggtaagacc gcaactggaa gttgaaggga acgtcccatt cgttgtagat atgctttcaa
     3121 tttttttcct cctcatgatt gttaacattt gtttgtacga ttgcgcttct taaacgctcg
     3181 atatggatgg ccaagtaacc cagttcttct ttcgtgatcg cgagctggta ttctttggtc
     3241 agcaacacac ggattttttt agaaatctta tagctttctg ggtattttcg tttgaccatt
     3301 tctacgattt ccgtgtctaa aacagcatat tgttgcgcat gggttcgttg tagaagcagt
     3361 cgtaaatgat tcaccagtct tgaataattc agtgaaaatg gttctgaatg gatatctgtt
     3421 tccaattctg attccactaa atggatgatg tcagatacaa tcgtcacttc acggatactg
     3481 cgatggttat tgttccgacc gcttcgtccg ctgtgaatat ggattgcgat atatcctgct
     3541 tcgtcataag tatatggaat cccaagctct tttgtcagat actctactgc ccattgagca
     3601 atactgaatt catcactgta aagcacttcg atttctctta aaagtttgtt gcggatgacg
     3661 ataccgttat taatgttctc cgcagaaaaa gcaatgtggt cggtcaaagc gatcaataca
     3721 tgttcattca acttttccat caaaacagtt tccgcatgat caatgatcgt ttctgctgca
     3781 aataaaaagc gttcatcaat ctgattaagt aaattttgca acttgatttg accttctggt
     3841 tccatgacaa atatccgttc gacatcacga tcaaaaatca aatcatttct ttttctgtca
     3901 aatccaatcc cttttccaat agctactttt tcctgtccac cgtcatctac caatactgca
     3961 ttttgattaa agatcttttt gattttcatg cgcgcaccct ttttgaagat ttggttttca
     4021 cgtttgctag accagttgat tgcggccaaa caaaaataga cacgaaaaac ctcctaggga
     4081 aaccatgttc ctcagagatt tttcatgcct agtatgatct agtcacatga aacgaattat
     4141 tgttaacgta atcaatttta tatagacagt tatgttttgt caattctttt tttataatta
     4201 tagtaattta ctggaattat cagaaataaa aggaagaaat aaattaagaa acttatgtta
     4261 tttaatcgtt ggatcttttg cttatcttat tcgttgttaa aaagctgaaa gcgaaggaaa
     4321 aaaactctac ttaactgtat ttctccgtta ggacatctaa aaatttacga gcagcaggag
     4381 aaagaggctg gtcttttttc caaatgagat tcagtgaagc ttgtaatggc ggatcgagtg
     4441 ggatgaatat tttttccgtc ccataagtat tgatgatccc atctagagct aatgcaccaa
     4501 aagtcccgtt atcgaccatc aaagaagcat tgtacaataa gttatatgtt ccgacgatcg
     4561 ataactgatc tatgctgaaa cctagccatt cagctagttg actgtccact gacgtttgcc
     4621 gggaaatgat caaaggaaca gttttcagtt cctgcggatg gatgacggtt tgctttgcta
     4681 aaggatgatt tcgatgtatc aaaagtcccc aacgatcttg ggctggcagt cgaaatgact
     4741 ggtatttttg tttgtttgtc ggttcgatta cgagtccaaa atcaagtaat ccgctatcca
     4801 gtttgtctat gatatcatct gcattcccgc tatataaatg aaactggata tcaggatgcg
     4861 ccttgatcaa cgaatgaagg ctatcagaaa tgaaagagat tgctttagtt tcgcctccgc
     4921 caatgtagat ttctccagag atgattgcgt catttgttaa attgttcgcc gttttttcta
     4981 ccaacgacaa gatttctttt ccttttgaat aaagatatcg accagaatcc gtcaactgta
     5041 tctctcggga gccccgagtg aaaagtgtca caccaagttc ttcttctaaa tctttcagtt
     5101 gttttgagag agtaggttgc gtgatatgca gagattttgc tgctcggctg atattttttt
     5161 ctctagctac tgctaaaaaa tacgtcagta tacgaatatc cattttttcc ctcctgtttt
     5221 taaaaaagca taccactaag tatagttaaa aggaatagtt aagtatgttt tataagtatt
     5281 agacatgata agccgtccgt tataaagtaa ggataaaagg agggagcaaa atgacaagcc
     5341 tcatcgaaaa acaaatcgtc aataaagaga ttcgagtaac ggatccgctc tttgaagtga
     5401 tccatcagat ccaagctgaa aacgaagagc ctttagcagc gctgaatact gggtaccatg
     5461 aaccagcaga tatccgcaaa cggctagaaa atatcattag tgacaaagta gatgatacgg
     5521 tgactgttct gctgcctttt tatacagatt tcggcaaaca tattagtata gggaaaaatg
     5581 tctttatcaa tcgtcaagtg atgttcgttg acttaggtgg catctgttta gaagattcag
     5641 tattgattgg tcctcgtgtc aacctcatca cggtgaacca tctaaccgat ccaaaagaac
     5701 gaagaggctt gttagtaaag ccaatccaca tcaagaaaaa tgcttggatc ggagcaggag
     5761 caactattct gccaggcgta acgattggcg agaatgccat tgttgcagcg aatgcaactg
     5821 ttacgaaaga tgtcccagac aataccatcg ttgcaggaat ccctgccaaa atcgtcaaac
     5881 ctgtcgaacg gattatttga acagtaagat gaaataagag atagtgagta taagcaagag
     5941 atttttcaaa acaggaggag aatagaatga atgatttatc agaaaaagtg attgtcatca
     6001 tgggcgcttc aagcggaatc ggtgaagcaa caacgaagct attagcagaa aaaggggcta
     6061 aactagttat agctgcacgc cgagaagatc gcctaaaagc aataaaagaa tctttaccag
     6121 aagctgagct atatattcaa acagcagatg taagagattt tgcacaagtc caagcagtta
     6181 tcgatttagc gatggaaaag ttcggtcgaa tcgatgtatt gtacaataat gcaggaatca
     6241 tgccaaccgc tccattagtt gaaggtcacc gtgatgaatg gcaaaatatg cttgatatca
     6301 acatcatggg tgtgttaaat ggaatttccg ccgtacttcc aatcatggaa aaacaaaaat
     6361 ctgggcatat catttcaact gattctgtgg cgggacatgt tgtttatcct gattccgccg
     6421 tttattgcgg aacaaaattt gctgtccgtg caatcatgga aggattacgt caagagcaac
     6481 ggcaaaataa catcaaatct acgatcatct ctccaggagc tgttcaaact gaattatatc
     6541 aaaccatcag cgacaaaaag gctgctcttg aacttcatga agcgcaaaaa gaatggggat
     6601 tgacctccga agatatagct tctgctgtag catttgcgat agaaacgcca gaccggatgt
     6661 ctgtcagtaa catgattatc cgtccgacga cacaggaagt ataatccata acggataata
     6721 tccaaagaga attacttgtt aaatagttat tcaaacgacc atcaatttaa ggcgtgaatg
     6781 gagaagaata ttcttcattt acgtcttttt atgtagaaaa agcaaattta tcaaaaagaa
     6841 acaaatgtta tttaaatatg gacattgact tgcttttact tttttagtta cactgttatt
     6901 taggaaaagt ttcataattc aaaggagaaa aaagatgaaa gcaaagaatt tattatttag
     6961 cgttattctt ttaggaaatc tagcagttgc caacacaaca gtgctagctg aagaatcgac
     7021 agaaactgtc gcaactcaga catcgagttc ttcagaaaca ggaattgaaa acgatacaga
     7081 gagtactttg cagtcagcta ctgtctcaga aaatacaatt tcaaccgaag aaccaacaac
     7141 tgaaacaaca gaacaaacac aaaccgaaga cagtacatca gcagaagctc agaacaatcc
     7201 agagaatgca gaagaaatca cgaaccaatt gacagatggg caaacattga cacataccca
     7261 tcaagcagat ttgtctgaat tcgaaaacta tactggctat gctttttatg ctcgctcatc
     7321 tgctaattct caacaagcgt tcattgatag tattgcctca actgcacaaa gtttggcaag
     7381 tgccaatgac ttatatgcat cagtcatgat tgctcaagcc atcgttgaaa gtggctgggg
     7441 gaacagtact ttggcttctg cccctaatta caatctattt gggattaaag gaagctataa
     7501 tggccaatct gttacgatgc ctacttcgga atatgtgaat gggcaatgga ttacggtgaa
     7561 tgccgctttt agaaaatatc cttcgtacaa agaatcattg caagataacg taacggtatt
     7621 gaagacgact tcttttcagc caggcgttta ctactattcc ggtgcatgga aaagcaatac
     7681 gaactcttac aaagatgcta cggcttggct aacaggtcgt tatgccactg cgcctaatta
     7741 tggttctaca ttgaacaatg taatcgaaac ctataatctg acacagtatg atacagcacc
     7801 aacttcaact tctccgatgt atcgtctgta taaccgtcat acaggagaac atttatatac
     7861 attgaatgcc ggagaaaaaa attatctgcc aactgtaggc tgggaatatg aagggattgc
     7921 ttggcaagca ccaaacagcg gacaacctgt ctaccgttta tacaatcctt acagtggaga
     7981 ccatcattac acaatggcac aatctgagat caactcttta acaaaaatcg gttggcgtta
     8041 tgaaggtcta agtttttatt ctggtggttc caagccaatt tatcgactat ttaatccaaa
     8101 tgaaaaaact ggtacccatc actatacctt gagtgctaag gaaagagatt ggctaacacc
     8161 gatgggctgg agatatgaag gaataggatt ttatggttat taaaaagagg tagtgaaaaa
     8221 gctgccctgc atcaagtagt aagaacagcc aaacaaaaac agattttcat gttttttgtt
     8281 tggttgaact tattaccgca gatgcaagtt tttgaacacc gtttactcaa aaaaggttgt
     8341 gacaacatct tgtcataact tttttattac tgtttaagca tactgatcca atttctaata
     8401 ttcgacggga ttcttgcaaa aggcgatttc ggaaattttt ggcggtgtaa aattttctac
     8461 tgcagaagag ctatcttcta tagtttgata agaatcaata atcttggttt tagcgttgtg
     8521 ctttctttat cattttttcc gttcattcta caaaaaattt cttttctcta tacttttcta
     8581 ttgagagcgt tttttttatt acacagctga ttctttgact tttttctttt tttattctat
     8641 tattgacaag tcaatcgttg acgagtcaat tattagaaag gaggaaatta tgtctttaga
     8701 agagattagt caccttttat atcaaatcaa attagccgat caaaaaatcg tccagatctt
     8761 tgaagaaaat atcggtataa gtcttactag atacgagatc atgatgagac tgaaagaaga
     8821 aaatccattg ctacaaagca gtttgcaaga aaagttgcag attgatcaag gagcagttac
     8881 tcgtcatctg aaggtattgg aagcaaatgg ctatgtttcg cgtcagagaa atcctcagaa
     8941 caaccgggaa gtgtatgtag cgctaacaga aaaagcccag aagaaaatcg agcattgtga
     9001 aatggaccat aaagatacag gttccgtgct atccccggct ttcagcgatg tagaaatgaa
     9061 acaatttcta acacttttag ataaattgaa tacccaactt tgcaatataa caacagaaac
     9121 gaaagaggtt aataataatg gaacaaaaaa atgatttttc aacaattatg actggtagaa
     9181 gatccgttcg cgtatatgat gaaacataca aaatcccaca cgaagaaatg ctggaaatga
     9241 tcaaagaagc gacgactgca ccttcttctg tcaatatgca accatggcgc tttgtggtcg
     9301 ctgaaagcga ggaagcaaaa gaaatcttgc gtcctttgat ccgtttcaat acgagacaaa
     9361 atgatacttc ttctgcaatg gtcatgattt ttggcgatat gcaatgttac gaatatggcg
     9421 aagaaatcta taaccaagct tacgaatcag ggaagatgcc aaaagaagtc agagatcaac
     9481 aattagctgc gatcattcct tattacaaga gtttttcaag agaagaaatg aacgatgtag
     9541 tcaaagtcga ttctagctta gcggctatgc aattcatgtt agttgcgcgt gatcatggct
     9601 atgaaacaaa tccaattggt ggagtcgaag cagatcagtt ggcagaggca tttggtttgg
     9661 ataaagaccg ttatgttcca gttattattt tatcaatagg aaaagcggta gaagaaggtt
     9721 acgagtctgt tcgtttagca ccagaaaaga tcactacgtt cgaataaaag gttttcagtt
     9781 actattcagt agctattgct ttttttgaat cgtgtgtggt aacctaataa aaaaggaggt
     9841 cggaaaaatg actgcatcga tgaaattacg tttcgaactt ttgaataaca actaaataaa
     9901 ttcaaaagac atgtataaac aacaatttca tggatggagt atgtccattt tttcgaacga
     9961 cagtagaata ggtgaaagct atgacagatt tggtgattta gtcatagtta tttcgttcag
    10021 agtaggaaaa gatgtgatgc agtttttttg caaacagatt tttctgctct tttttagtat
    10081 cgataattgt tgttttacat gaaataggag tgtaaaacat atggaaaatt tagcagtaaa
    10141 tataacaaat ctacaagtca gctttggcaa ccagctagaa ctatctattg attctcttcg
    10201 tgtctatcag caagatcgta tagggatcat aggagaaaat ggtgtcggta aatcgacttt
    10261 gctcaaacta atagccggtg aactttttcc cgatcatggg aaaatccaaa cagagatcac
    10321 cttcaactac ctgcctcaat taacctatct cgctgaagca aaggacctaa atttggaatt
    10381 agccagtcat ttccagttaa gactggaaga aacttcagag cggaaatgga gcggagggga
    10441 agaacgaaag atcgagttga tacgtcttct ttcttcttat gaacaaggga tgcttctaga
    10501 cgagccaaca acccatctag atagaaaaag tattgatcga ctgattgaag agcttcgtta
    10561 ttattatggt acgctggttt ttgttagtca tgatcgctat tttctagatg agctagcatc
    10621 gaaaatctgg gaagtaaaag acggagaaat ccgagagttt tcggggaatt atagtgccta
    10681 tctcactcaa aaggaattgg agaaaaagac tcagctacga gaagcagagt cgatcatgaa
    10741 agagaaaaaa cgattggaaa aatcgatcca agaaaagaaa aaacaagcgg aaaagttgga
    10801 aaaagtgtcc agtaaaaaga aaaagcaaca aatcagaccg gatcggttgt cttcctctaa
    10861 acaaaaagac agtgtacaaa aagccatcca aaagaatgcg aaaacattag agagaagact
    10921 ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa caaatccgtt ttccagtacc
    10981 aaaatctctt gaactccaca gccgctatcc catcatggga caaaatgtcc aattggaaag
    11041 aagtgggaga acattactgg taaatggtga ttttcagttt tctttaggta aaaaaatcgc
    11101 gattgtcggt gaaaatggtt caggtaagac aaccttatta gaacatatcc gcaaacaagg
    11161 agaaggaatc cttctctctc cgaaagtaag ctttcaagta tatcagcaaa agggttatca
    11221 aatgacatct gaagaatcca tcattcgttt tgtcatgaga caaacagagt tttcagaatc
    11281 gcttgtccgt agtttgctga atcacttagg gtttgctcag gaaactttga cgaaaccgtt
    11341 atgtacatta agtgggggag aagcgacccg tctgacgatt gctttgcttt ttactaagcc
    11401 aagtaatgtg ttgctgttag atgaaccgac taattttatt gatatggcaa cgatcgaagc
    11461 tttagagaag ctaatgcaaa tatatccggg aacgattttg tttacttctc atgattctta
    11521 ctttgtcgag cgtacggctg atgaagttta tgaaataaaa gggcagaaaa taaaaaaagt
    11581 acttacgaga aatttttaaa tgtgctacga tgattttcgt gacaaattta aaggagtggt
    11641 acagattgcc tacaaataaa aaagaaggaa taatttttac aacaatgatg tgttttttga
    11701 tggtcttggg gatgagcgcg tataatttat ggctgcatca agatcttcat ttatcaagtt
    11761 tgttgattgg tctagtgcct ggattcgtgg tggctttcat tttggatgtc ttcatcatcg
    11821 gtgtgctggc gaaaaaaatt gcctttaaac taccagtaaa tccagaaagc aaggtccaaa
    11881 tgatcttaac tatttcatgc ttgatggttt taggaatggt gacatgtatg tctttattcg
    11941 gtgtgatcat ggaacaagga atacctgatc aattattctc tgcttatatg aatgcttgga
    12001 aaatgaatgt gattgccgct ttaccgttgc agttgatcat cgttggcccg ctttcaagaa
    12061 gtgtgctaaa agcaattcaa agtaatgaat gatggaacac tgttatttga aaaaaggcct
    12121 tggcatgctt tttgtcatag tctcttgaat gcagttctag aaaaatagtg atccgaacga
    12181 taggttttgg cctgtcgttc ggatcgcttt ttcttttcca actgtattgc tcttttattg
    12241 aagataattt gctcttcaat agatatttct agaaaggttc tgtttatccc ttactcaaaa
    12301 aactgctgca tttgttctct tacttgctga caccattgga gttcctgctg acaaagcata
    12361 attctgcgct ggatcacaac ataacgtcca aattcttgtt tcttgtctag cgagaggtca
    12421 ttcaattttt tttgattttt ttgcatgatt ttgttcagct gttgttctct agtgaatagc
    12481 agtccagtag ctgttgtttt atcgagcgtc gaaaaagcat agatttttgc tagaaattca
    12541 tctttttgcg taggagcggg agtttcctct ttgatccatt cctctacaag agcacgtcct
    12601 tcatcagtca agtggtagat ctttttttga gagtgctcgt ctttgtccaa aacggtcaca
    12661 taaccctgct ggtctaactt ggcaagcgtt gtatagatct gactgtgatg ggcttcccaa
    12721 aaaattgtga tcagctgctt caattcatac ccagaacgtg gtttggataa aagcacgcat
    12781 aataatatat aggctaaagt attcataaaa atcctacctc tcgtcttatt ttctttatta
    12841 tatcacagta aagtttccac gttttacatg tgcatattta catgtaaaat aaatcatgta
    12901 tgatgctaat agagagaaag ggatggaaat attggagaat caaatagata tacaagaaca
    12961 aacaaatgaa gaaacaaagg ggaaaagttc agcgctagtt gtagcagcta cctgtatgat
    13021 cgcttttatg ggagtgggtc tagtagaccc aatcttaaaa acgattgctg cacagttaaa
    13081 cgctacgcca gctgaaacga ccctgttatt tactagttac atgttagtca ctggtgtggt
    13141 gatgctattt acaggtttct tatctagtcg gatcggtttg aaaaaaacat tgatgacagg
    13201 attgtttatc atcgttgtat tcgctggtct tggcggatta tctgggaata tcggaatgct
    13261 gattgggtta cgtgccggct ggggtgtcgg gaatgcttta tttgtctcta ctgctttagc
    13321 aacgatcgtc agtattttag ttggtaacac agaaaaagca atcatgatgt atgaagcagc
    13381 acttggctgc ggaatggctg ttggccctct agtcggtggg gtcttgggca gtggttcatg
    13441 gcgttatcca ttttttgggg tagcattttt gatgttttgc ggttttatag ccctagcggt
    13501 acttttacct gaaactgaga aaccaaagag aaaagtaggc ctttgggaag gtgtcaaagc
    13561 gttaagcaat cataaacttc gttcagttgg actaatcgca ttgttatata atttcggatt
    13621 ctttacttta cttgcgtatg cgccattttt attgattggc tattctgaat tagaagtcgg
    13681 ttttgtattt tttggctggg gtgtgcttct ggctattgct tcgattttcg ttgcaccggc
    13741 tcttgagcgg aagtttacga cttatcggac aattttagca gcatttgtct tattcattat
    13801 ttgtttgctg ttattaggag ctggaacttc tatgccagtc cttgtaccga tcagcattgt
    13861 gttagcagga tttttccaag gaattatcaa tacactgttg acgacgattg ctatggaaat
    13921 accaggtctg caacgaaacg ttgcttcgtc cagctatagt tttgtccgct ttttcggtgg
    13981 agctttagca ccatttattg caggtgtgat tggtgaacat attggtgaaa gctatgcgtt
    14041 ttattttgca ggtgccattg ttcttatcag tgtatgttgt atcatttatc accgtaatta
    14101 ttttgctaca aatgaagaag aataagaaat cagaggttgt gaaaaagctg tcctgcatca
    14161 agtcataaga a
//
LOCUS       DAZUKT010000274         9921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.099, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000274 DAZUKT010000000
VERSION     DAZUKT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.099"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..357
                     /locus_tag="ACG816_003221"
     CDS             187..357
                     /locus_tag="ACG816_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4921999.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            371..973
                     /locus_tag="ACG816_003222"
     CDS             371..973
                     /locus_tag="ACG816_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4922000.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            989..1666
                     /locus_tag="ACG816_003223"
                     /pseudo
     CDS             989..1666
                     /locus_tag="ACG816_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1977..2474
                     /locus_tag="ACG816_003224"
     CDS             1977..2474
                     /locus_tag="ACG816_003224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS4922001.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2475..2891
                     /locus_tag="ACG816_003225"
     CDS             2475..2891
                     /locus_tag="ACG816_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS4922002.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            2911..4455
                     /locus_tag="ACG816_003226"
     CDS             2911..4455
                     /locus_tag="ACG816_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4922003.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4598..4822
                     /locus_tag="ACG816_003227"
     CDS             4598..4822
                     /locus_tag="ACG816_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4922004.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            4837..5706
                     /locus_tag="ACG816_003228"
     CDS             4837..5706
                     /locus_tag="ACG816_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4922005.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5687..6421
                     /locus_tag="ACG816_003229"
     CDS             5687..6421
                     /locus_tag="ACG816_003229"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4922006.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6454..7362
                     /locus_tag="ACG816_003230"
     CDS             6454..7362
                     /locus_tag="ACG816_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4922007.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7359..7901
                     /gene="sat4"
                     /locus_tag="ACG816_003231"
     CDS             7359..7901
                     /gene="sat4"
                     /locus_tag="ACG816_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4922008.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            7994..8788
                     /locus_tag="ACG816_003232"
                     /note="aph(3')-IIIa"
     CDS             7994..8788
                     /locus_tag="ACG816_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4922009.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9064..9195
                     /locus_tag="ACG816_003233"
                     /pseudo
     CDS             9064..9195
                     /locus_tag="ACG816_003233"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9271..9764
                     /locus_tag="ACG816_003234"
                     /pseudo
     CDS             9271..9764
                     /locus_tag="ACG816_003234"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3494 a         1556 c         2169 g         2702 t
ORIGIN      
        1 ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc gaagcgaaca
       61 cttgatcttt taagttgcta tctttttaag gtcaatagag tatacttatt tgtcctaata
      121 tggttttagc agtataattg acttggtgaa taggtcattt aagttgggca taataggagg
      181 agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt ggctattttt
      241 aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc tgataagcaa
      301 tttgaaattg aaaaagtaga tatagctgat tggttattgc aaccgagaga attttagagg
      361 ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac agaacttaga
      421 tagacaactg aaagcgttag agggcgtttc taaggtcttt tcagacaaat taagcggtca
      481 atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag gggatatagt
      541 tgttgttact gaattagatc gattaggacg aaataataaa gaattaacag aattgatgaa
      601 tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa tgaatggtat
      661 tgaagatgaa aatttaagac ggctgattaa taatttagtg attgaattgt ataagtacca
      721 agcggaatct gaacgcaaac gaattaaaga acgccaagcc caaggaattg aaattgctaa
      781 gaaaaaagga aaattcaaag ggcgacaacc taaattcaaa gaaaatgatc cacgtttaca
      841 acacgctttc gatttatttt tgaatggttg ttccgataaa gaagttgaag aacaaactgg
      901 aattaatcgc cgaacgttta gaaggtatcg atcaagatac aacgtgacag tagatcaaag
      961 aaaaaaacaa tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaaccaag
     1021 ccaggcatta gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga
     1081 gatcaaagac ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt
     1141 cgaattagca gaaccaggtc attataacga aaagtggcag aactggaaac ttgaatcttt
     1201 gccgattttt cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa
     1261 aattgttgct gaacttctca aaaaggcaaa tacaattatt gtcgcaacag atagcgacag
     1321 agaaggtgaa aatatcgcct ggtcgattat ccataaagca aatgcctttt ctaaagataa
     1381 aacgtataaa agattatgga tcaatagctt agaaaaagat gtgatccgaa gcggttttca
     1441 aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat
     1501 tgccgattgg ttgatcggca tgaacgcaag ccctttatat accttgaatt tacaacaaaa
     1561 aggcgtacaa ggtacatttt cattaggacg tgtacaaaca ccgactttat atctcattta
     1621 tcaacgccag gaagcaatag aaaactttaa aaaagaacct tttttcctga ataattcata
     1681 gttttactaa tagtctttcc taacattgat ttaatagtct ttaaagtatt ctgcttcatc
     1741 acccattagg tgatgaaaaa agaacccctt tctgatttag cggacaaata ctttgaccta
     1801 atctcagaag atataaaaga aaaaataata aaatatccga tttatacttt gtataaagaa
     1861 ctaaagtagt tagcaaatca tttacagcaa atcactatgt tccacatacc attttacata
     1921 cttaccttat ccacgacaca tgttgagata gtatcgaaga taaagaaagt tgctccatgt
     1981 tgtttttgcg agatggggaa atcaaagcca cattgacaac catgatgaat aagcttgctt
     2041 tcagtcataa gctaatccta gaaccacttt tcaagtcagt tagccaaatc gatgaagaaa
     2101 gcgaccgtga aagaatggac gctattgata agctaatgga gcaactattg gaagaacgca
     2161 atacccttat tgcactgatg tccaaaggtt tcctagaacc tgctcttttt aatcaggaac
     2221 gaaatgtttt agatagtgag attaaaaatc ttactaccga aaaaacaaac ctggtaacga
     2281 actctgcgag tggggttttg cgagcaaacg agataaagga cctcattaat tatgtgtcag
     2341 cagataattt taatggtgac tacacggaag aactatttga agaatttgta gtgaacatca
     2401 ttgtaaattc cagggatgag ctgacattca atttgaaatg cggtctttcc ctgaaagaaa
     2461 aggtggtgag ataaatggca tatattccat atggatacaa aattcaagat ggagtggtta
     2521 ctgtcgatga aaaggcagca ggtcaagtaa aggtattctt tgagaaatac atatcaggac
     2581 tatcccttac agtggctggc gaacaggcag gtattgaaaa gacacattct gtgatggggc
     2641 gcattttgaa aaacgtcaac taccttggaa atgatacgta tccagcaatt attgataaag
     2701 agatatttga taaagctgaa gaagttagag ataaacgtgc aaaggattta ggacgagtgg
     2761 tagagcttgc cgctttcacc tctccccctc ccaaagaacg attcaaaatg aaaaaggcag
     2821 ataataagat gccagtggat ccttttgaac gagcagaata cttatacagt ctgatagaaa
     2881 gcgaggaata aagtgacaga gaaaaatata atggttattc ctgctcgtaa aagagtagga
     2941 agtacagccg caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg ccgtgtttct
     3001 acagaaacag aagaacaaaa ttctagctat gaggttcagg tctcacacta tacagagttt
     3061 ataaagaaaa ataatgaatg ggagtttgct ggcatctttg cagatgatgg tatctctggt
     3121 acgaacacta aaaagcgtga cgaatttaat cgcatgattg cagagtgtat ggacggtaac
     3181 atcgacatgg ttattacaaa atccatcagc cgatttgcgc gtaacaccct agactgcctt
     3241 caatacatta gacagctcaa ggataagaac atatcagttt attttgagaa agagaacatc
     3301 aacaccacgg atgccaaagg tgaggttttg ctgactatta tggcatcttt ggcacagcag
     3361 gaaagccaga gcctttcaca aaacgttaag cttgggctac agtaccgata ccaacaagga
     3421 aaggtgcagg tcaaccataa gcgatttatg ggctacacga aagatgaaga tggaaattta
     3481 actattgtcc ccgaagaagc tgagattatc aaacgcatct accgagaata ccttgagggt
     3541 cagagtctag tgggcattgg tcgagccctt gaaaaggatg gtattttaac agcagcggga
     3601 aaaccaagat ggcgaccaga atcagttaag aagatacttc aaaacgaaaa atacatcgga
     3661 gatgcccttc tgcaaaagac tgtcacagta gattttctga ccaagaaacg agtgaaaaat
     3721 gaagggcatg ttccccaata ttatgttgaa aatagccatg aagcgattat tcctaaagag
     3781 ttatttttgc aggcgcaaga agaaattcat cggagaagca atatctacac aggagaaggc
     3841 aagaacaaaa gaatttacag tagcaagtac gctttaagtg ccatcacctt ctgtggagat
     3901 tgtggcgata tttatagacg ggtctattgg aatatccacg gaagaaaaga atttgtctgg
     3961 cgatgcgtga ctagaatcga gcaaggtcct gaagtttgta agaaccgaac cgtaaaagaa
     4021 gatgaacttt atggtgcagt aatgactgcg attaataagc tacttgcagg tgggaacaat
     4081 atgataaaga cactggaaga gaatattcat gctgtgattg gcgaaaccac agaataccaa
     4141 atttcagaga ttaacaactc actagaggaa aaacaaaaag aactaatcaa gctggcgaac
     4201 aagggtcaag actatgacca cttagcagat gagattgatg agctgagaga caagcgacag
     4261 atccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt
     4321 gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag
     4381 ataatccaga gcgttacagt ctatgaaggc cacttcgtca tagccttcaa atctggcatc
     4441 gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctacagtag agttgtgggt
     4501 tttcttttgc ttgctgatta taataatatt ttaataacta ttgtgtttat caaccaaatg
     4561 gtttataata ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa
     4621 agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac
     4681 tccacagaat ttcaataaaa aattacaacg agaaacagta accttggatg agttgaaggc
     4741 cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagaaga
     4801 aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata
     4861 gtaacagaga aattatcatc tttgcctttt atagaaggca tcgtattagg gggctcacgt
     4921 gcaagaggca cccatacaga aaattctgat atagatatcg gaatctatta caattcagac
     4981 tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga gaatagaaac
     5041 aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg atggttagtt
     5101 ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga acaaataatc
     5161 aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat
     5221 ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa
     5281 agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg
     5341 atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg
     5401 gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa
     5461 gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt
     5521 gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta
     5581 ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa agaagtgaat
     5641 gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa
     5701 atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact
     5761 ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg
     5821 tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc
     5881 ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac
     5941 ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga
     6001 gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt
     6061 aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca
     6121 tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact
     6181 gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga
     6241 aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag
     6301 ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg
     6361 gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta
     6421 ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat
     6481 ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca
     6541 cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta
     6601 agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata
     6661 atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat
     6721 cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta
     6781 gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt
     6841 aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag
     6901 tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc
     6961 cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg
     7021 agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac
     7081 tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac
     7141 cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg
     7201 gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat
     7261 ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat
     7321 agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag
     7381 ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     7441 cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa
     7501 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     7561 acaatactga taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta
     7621 aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt
     7681 tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata
     7741 aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct
     7801 atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     7861 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     7921 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     7981 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     8041 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     8101 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     8161 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     8221 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     8281 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     8341 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     8401 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     8461 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     8521 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     8581 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     8641 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     8701 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     8761 ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa aagctttaac
     8821 tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc catactctga
     8881 tgttttatat cttttctaaa agttcgctag atagagttct atattagaga tgtaaagagt
     8941 tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag aagcactaac
     9001 agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga ggaggaataa
     9061 aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc aaaaaaaata
     9121 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     9181 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     9241 cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg
     9301 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     9361 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     9421 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     9481 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     9541 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     9601 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     9661 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     9721 tggaacaaat attaaaacaa atgctttcat ataaaaaact atatgcccta caatgccgtt
     9781 gtaaaaattg gggaaaaggg atatattgat tttatatctt tctcaaaata aatataaaag
     9841 ccctttaaag agggctttta tatattaatc acaaatcact tatcacaaat cacaagtgat
     9901 taatcacaaa tcacttgtga t
//
LOCUS       DAZUKT010000275         8160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.113, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000275 DAZUKT010000000
VERSION     DAZUKT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.113"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG816_003235"
     CDS             complement(134..577)
                     /locus_tag="ACG816_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4922010.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG816_003236"
     CDS             complement(795..2135)
                     /locus_tag="ACG816_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4922011.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG816_003237"
     CDS             2404..3300
                     /locus_tag="ACG816_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4922012.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG816_003238"
     CDS             complement(3450..4391)
                     /locus_tag="ACG816_003238"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4922013.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG816_003239"
     CDS             4517..5638
                     /locus_tag="ACG816_003239"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4922014.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG816_003240"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG816_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4922015.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG816_003241"
     CDS             7465..7992
                     /locus_tag="ACG816_003241"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4922016.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2709 a         1514 c         1605 g         2332 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
//
LOCUS       DAZUKT010000276         7889 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.115, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000276 DAZUKT010000000
VERSION     DAZUKT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.115"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..1495
                     /locus_tag="ACG816_003242"
     CDS             59..1495
                     /locus_tag="ACG816_003242"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4922017.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1684..4131
                     /locus_tag="ACG816_003243"
     CDS             1684..4131
                     /locus_tag="ACG816_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4922018.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4361..5428
                     /gene="rlmN"
                     /locus_tag="ACG816_003244"
     CDS             4361..5428
                     /gene="rlmN"
                     /locus_tag="ACG816_003244"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4922019.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5418..5624
                     /locus_tag="ACG816_003245"
     CDS             5418..5624
                     /locus_tag="ACG816_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4922020.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5621..6724)
                     /locus_tag="ACG816_003246"
     CDS             complement(5621..6724)
                     /locus_tag="ACG816_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4922021.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7276..7824
                     /gene="aac(6')"
                     /locus_tag="ACG816_003247"
     CDS             7276..7824
                     /gene="aac(6')"
                     /locus_tag="ACG816_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4922022.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2647 a         1345 c         1721 g         2176 t
ORIGIN      
        1 gggtttcgtg ttattctaaa agaggaacga ttgaaggtta taggaaagga agagatgaat
       61 ggaacacaaa gaacttacct catttccaaa agattttctg tggggctctg cttctgcagc
      121 gtatcaagta gaaggagctt ggcaagaaga tgggaaagga gaatcagtct gggatcgatt
      181 tgtacggata cctggaaaga catttaaagg aacgaacggt gatttggcag tagaccacta
      241 tcatcgttat aaagaagata ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt
      301 ctctgtagct tggacacgga tttttcctaa tggacgagga gaagtcaatc aagctgggct
      361 cgctttttat gagagattga tcgacgaatt gattgaaaac gatattgaac ctatgttgac
      421 tttatatcat tgggatcttc cacaagcatt gcaagatgaa tataatggtt gggaatcacg
      481 tcagatcatt gaagatttca ctcattatgc agaaacatta tttgaggcat tccgcgggaa
      541 agtccattac tggattagtt tgaatgaaca aaatatcttc acttcattag gttatttgtt
      601 agcagcacat ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta accatattgc
      661 taatttagca aatgcatctg tcatcaataa atttcacgag ttagaaatgc ctggaaaaat
      721 cggaccaagc tttgcgtata cgccaaatta tccaattgac agtaatcctg aaaatgttct
      781 ggctgctgaa aatgcagaag acctgatggc caattattgg atggatgtct atatgtgggg
      841 aaaatatccg attgctgcca tgaggttctt agaagaaaaa ggatgggcac caacgattga
      901 agctggcgat gcagaattgc tagaatctgc taaaccagat tttcttggga tcaattacta
      961 tcaaacggcg acgaatgcat tcaatccatt aaacggagtt ggtgctggaa aaatgaatac
     1021 gacaggaaaa aaaggttcat cagaagaaac tggagttcct ggaatgtata aaaaagttga
     1081 gaatccattt gtcgaacgaa caaattggga ctgggagatt gacccagaag gattaaggat
     1141 cggactgcgt cgtatcacaa gccgttaccg catgccggta ctgattacgg aaaatggctt
     1201 aggagagtat gacaagttga cagaagataa acaaatccat gacgactatc gaatcaatta
     1261 cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg gatggagcag aagtcctagg
     1321 ctattgcacc tggtcttata cagatttgct tagctggctg aacggttatc aaaagcgata
     1381 tggatttgta tatgttgatc aagacgaaac acaagaagga tctttggaac gatataagaa
     1441 agacagttat tactggtacc aaaaactgat tgaagaaaat ggagagaacc tttagttcac
     1501 atacttaaaa caagaaggta cggctggata tggccgtgcc ttttcttgat aaaatcattt
     1561 agaaattaag aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa
     1621 acaaaacttt agttccctta cttttttttg tataatgaaa cttgatgaaa tgagggataa
     1681 taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag
     1741 caaaaacagc agcaggaaca aatgctgacg attggtgttg ggtttgcctt gattttattt
     1801 gctgtcttcg gctttttaaa attaggtttt ttaggaatat tgattgcaaa cggctttcgg
     1861 atcattgcag gaaatactta ccaaatcctc tgtcttttat tagccgtatt aggattttgg
     1921 attgtcatta agaatacaga gttttcaatt ggtaaaaacc gacgctggtt tggcggcata
     1981 ctcttttatt tcggtatctt attattgtta catgcccatt tatttggcaa actccatact
     2041 ggggaaccaa atatcatggg gacgacatgg gatttgttag ccagtgatat aaaacaaagt
     2101 caagtagaca ataacgttgg tggtgggatg attggagcaa tcctttatca ttttacttac
     2161 ttcttgattg cacagcctgg aagttattta gcagcagtgc tattactggc aggaggagca
     2221 ttcctgttca gtaatctgga aggctatcag ctgttaaatg gtatccaatc aattggagaa
     2281 cgagtacagg aattattgga aggtgatcct gccaagcaag ctcgaaagca agcggcaaag
     2341 gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa
     2401 gctgcagaaa aagaagctgc agaatatgag aagaaaaaag cggtgagcca gcaaagggaa
     2461 aatactgctg atgaatggca ggaaccagaa aatacagagc cggagcagct aagttttgtt
     2521 ccaatcgaca gttttcaaga aaatatccat cctgcgaatc tagagaaacc agtaccagat
     2581 acaccaaaac agaccaatac agcagaaggt ttttcagatg agttgccaga ggatgacggt
     2641 acctcattag aatttgagat tgaagcagaa caggaaaatc aagactatga attaccgaca
     2701 gttgatttgc ttgattctat accaactgtg gatcaaagtg atgagtataa aaaaattgaa
     2761 aaaaatatcg gcgtattaga acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc
     2821 aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa
     2881 gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg ctttagcggc gaaagatgtc
     2941 cggatggaag caccgattcc aggtaaatca ctgattggta ttgaagtacc aaatggaaag
     3001 atcagtatgg tttcctttag agaaatcatc gaagcacaac caaaccaccc agataaatta
     3061 ctagaagtac ctttaggacg tgatgtttct ggacgggtac aaacggctga tctttcgaag
     3121 atgcctcact tactcgtggc tggttcgact ggtagtggga agtcagtagc aattaatggg
     3181 attatcacaa gtatattaat gcgtgctaag ccccatgaag taaaactgat gatgatcgat
     3241 ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc atctgttgac gccggtcgtg
     3301 acgaacccta gaaaagcagc gcaagcctta caaaaagtag tacaagaaat ggaagaacga
     3361 tatgaaaaat tcgcggcaac cggagtaaga aatatttctg gttacaatga atttgttcaa
     3421 cagaaaaact tggaaaacgg aacgaaacat ccaaccttac cattcatcgt agtgatcgta
     3481 gatgaattag ctgatttgat gatggtagcc agcaatgaag ttgaagatgc gattattcgt
     3541 ttagcgcaaa tggctcgggc agcggggatt catatgatcc tagctactca acgtccaagt
     3601 gtggatgtta tcacaggaat catcaaagca aatgtacctt ccagaatggc atttgcggta
     3661 tcaagcggta cagattctcg gacgatcatt gattctaatg gggcagaaaa gctcttaggg
     3721 cgaggagata tgctattctt gccaatgggt gagaataaac cgatccgtgt gcaaggagca
     3781 ttcatttctg accacgaagt agaacgggtc gttcaatttg ttacagatca gcaagaagct
     3841 cattatgaag agaaaatgat gccgacagat gaagttgaaa cgacaggtgc tccagaacag
     3901 ccacaagatg agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt
     3961 atttcccttc tccaaagaag atttcggatc ggttacaacc gagcagcacg tttagtagat
     4021 gaactggaag ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc
     4081 attgagcagg aagaaacatc agaaacgttg ccggacgata atgacatgta aaacttgtgt
     4141 gatgaagggt tgaaaaacga ataaccaata aaaagacaag gcatgatgat accaaaagaa
     4201 aaaatggttt ttttccatct tttctaatac ggtatgatgt cttgtctttt tagtttttgt
     4261 gagattaaag ttctattcga tttatttgtg aagtattgca ttaaggaaat gaatcatgta
     4321 tagtatgtag cgaaactttg agaaaaagag ggaaaatact ttggaaaaac agtccattta
     4381 tggcttgacg aatgaagaat taatcaattg gtttatagaa aacggcgaga aaaaattccg
     4441 agctgcccaa gtttgggaat ggttgtatca aaaacgtgta agtaatttca cagaaatgac
     4501 gaatctatcg aaacaattga tcgaaaaatt atcggagcat tttatcatca atcctctaaa
     4561 acagatggtc gtccaagaag cttcggatgg tacagtaaaa tatttgtttg aactccctga
     4621 taaaaacatg attgagactg tactgatgag acaagaatat ggcttgtcag tttgtgtgac
     4681 gacacaagta ggatgtaata tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa
     4741 ccgtgatctg actgctggag aaattgttgc acagatcatg atggtccaac attattttga
     4801 tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta atggggatcg gtgagccatt
     4861 tgataattac gacaatgtaa tggacttttt gcatatcatc aatgatgcca aaggattggc
     4921 aattggtgct cggcacatta cagtttccac tagtggatta gcacacaaaa tcaaggaatt
     4981 cgctaataac ggcttgcaag tcaatctggc tatttccttg cacgcgccga ataatgaggt
     5041 gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt gagaaattga tggaagctgt
     5101 ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt gaatatatca tgttgaatca
     5161 agtaaatgat agaccagagc atgcgcaaca attagcagat ctattaaaag ataagaaaaa
     5221 attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg
     5281 cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg
     5341 tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa
     5401 gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa
     5461 aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag
     5521 cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca
     5581 atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt ttcttatatc
     5641 cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc tgtttaaatt
     5701 tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg gctttatcta
     5761 agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat actggaagtt
     5821 ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga tccatcatca
     5881 ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc attttttcta
     5941 gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta ttgttaacta
     6001 aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca attgcatatg
     6061 agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag agacgatcaa
     6121 tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca tcgccacgag
     6181 cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc cataagtatt
     6241 tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt ggttttgaag
     6301 gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc gcagtgacgg
     6361 gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt ttttcaccaa
     6421 tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg tctgcttttt
     6481 ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta cccccgtacc
     6541 agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg ttttctgcct
     6601 ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct ttgacgattc
     6661 catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac
     6721 tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc
     6781 tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt
     6841 ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta caaaaacatt
     6901 gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc ctttgctttt
     6961 ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg ttttatttgt
     7021 caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg aacaaacaga
     7081 gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa gtgtttctgt
     7141 ataatttttt attttataag taagataaat aatgaaagaa tagagatact gattggtagc
     7201 taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa
     7261 caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat
     7321 cagctttctg atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa
     7381 gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag
     7441 ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca
     7501 ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta
     7561 gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac
     7621 catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc
     7681 atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta
     7741 ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt
     7801 attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag
     7861 aaataagaag gaatttacga aaattactt
//
LOCUS       DAZUKT010000277         6798 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.122, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000277 DAZUKT010000000
VERSION     DAZUKT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.122"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(516..743)
                     /locus_tag="ACG816_003248"
     CDS             complement(516..743)
                     /locus_tag="ACG816_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4922023.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(752..1225)
                     /locus_tag="ACG816_003249"
     CDS             complement(752..1225)
                     /locus_tag="ACG816_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4922024.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            complement(1865..2038)
                     /locus_tag="ACG816_003250"
     CDS             complement(1865..2038)
                     /locus_tag="ACG816_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4922025.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            2455..3021
                     /gene="amaP"
                     /locus_tag="ACG816_003251"
     CDS             2455..3021
                     /gene="amaP"
                     /locus_tag="ACG816_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4922026.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3034..3222
                     /locus_tag="ACG816_003252"
     CDS             3034..3222
                     /locus_tag="ACG816_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4922027.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3235..3792
                     /locus_tag="ACG816_003253"
     CDS             3235..3792
                     /locus_tag="ACG816_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4922028.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3824..4066
                     /locus_tag="ACG816_003254"
     CDS             3824..4066
                     /locus_tag="ACG816_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4922029.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4094..4321
                     /locus_tag="ACG816_003255"
     CDS             4094..4321
                     /locus_tag="ACG816_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4922030.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            4407..5798
                     /gene="amt"
                     /locus_tag="ACG816_003256"
     CDS             4407..5798
                     /gene="amt"
                     /locus_tag="ACG816_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4922031.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            6193..6688
                     /gene="dfrF"
                     /locus_tag="ACG816_003257"
                     /pseudo
     CDS             6193..6688
                     /gene="dfrF"
                     /locus_tag="ACG816_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
BASE COUNT         2339 a         1022 c         1332 g         2105 t
ORIGIN      
        1 ggattttttc atttacacaa aatattttat actctccact gttattcaaa tcattttatc
       61 aaaactacat agtaaatgtt aaaaagcaac atgacataat atatagaagg ctttacagta
      121 tatatgggag cttactaaaa aagaatggag taagagttaa cacaatgggc cattagctga
      181 agttttaagc acttcaaggt atgaaacaga atggcctgta atactacatg attatttaga
      241 ctgctcaaac tgctatcagt ctggtggaat gaataaagta ttatactaaa agcagatcca
      301 actacaacgt aacaggaaac cagttgtatc aggaaaagtg atagtacatg gagaagaaaa
      361 ggcccccttt gtatgagtca gtacatatct tatttataga taaaacggta ttcaatcgaa
      421 ataaaaaatc tgaataatag acgtaaataa cttctaaaag tctcacacat ctatcattca
      481 gattattttt tatttactgt ttgttctact ttattttatt cttctggtat agtattcgag
      541 tcatttttat aggtcaacaa atcacctggt tgacactcca aaacttggca gattttttcg
      601 agagtggaaa aacggatggc ttttgctttt ccatttttta gaatagagat gtttgccatc
      661 gtaatgccca ctttctcact caattcagtc acactcattt ttttttgagc taatactaca
      721 tctaaattaa cgataattgc catgtcgcac ctcacactgt caaatcatga tctgtcttaa
      781 gtaaaatcgc atgttcaagc agtttttcca gcacgcttgt taatgtgtat actgcaaaag
      841 gaatgaatac gacagcgatt cctaccaaca tcaaacctgg agcatcgtct aaatccgcta
      901 ctttcataaa tttaggaagt aagccaaaaa aggcgaaacc aataaacaaa atggttcgtt
      961 cgattttttt tagtatgttt aagctttgag tggaaaaagc ttggttatct tcgatttttt
     1021 tcaatagttt caataagaaa taagcaataa cgatccctag gagaagggca aagtaaatga
     1081 cagaaacaaa taatgtggtc tgccaatcaa aattgaatcc tttttcgctt gtagcgatct
     1141 gagtataaaa aagtacgccg aacaaaataa atacggcact tatacctaaa acgacaaatt
     1201 ttaatagcaa tgttttaaat ttcattttta tccctctttt atctttgata gctaaaatat
     1261 agcagaataa ttattgtaaa acaactattt tttatcgaaa aacaataaaa atatatcgtt
     1321 tataaaaaat gatagagtat gaaggtgtgg agtgcattta acagcttttt ttgattgtaa
     1381 gaaacattta aaaccagtta ttttagattc tatgtgtgtc tcttctataa tgctagttta
     1441 gatgcctggg attatattgg acaatacagt tggaggattt tttattatct agcgaagaga
     1501 agtgtaagtt aacagaactg tatgagaaaa aagacaacta cgtagtagct aaccgattca
     1561 ttaatttttt caagttcatg tttatttacg tatcagaatt tttcttattc aatggtttgg
     1621 tgtaatcacc acttatttgt cctttattgc ttatacaatg ttatacaaaa ggaaaatcaa
     1681 gcattgacgc atggggtggt actatctagc tgaaatagct taataagcgc aagtagatgc
     1741 attggattag tttataaatc aaaagtgtta gaggtattaa gaagaagtag aaaaataaaa
     1801 tggattgccg aataaaaagt accataaatc ctaaaacatg tgccaacaga tagatggcac
     1861 atgtttattt ttttcgataa ttttgtaaca aaaatacgaa gatgaaaaag aatagagatt
     1921 cccataatgt agtagtccaa ctgaattctt tactgatacc aaataccata tttcctagat
     1981 ttgatacaat caaatagcca atcgttatga tcaatgaata gtacaatctt ttcatcatag
     2041 gacatacctc atttttattt ataagaatag tataaaccaa agaagtgaat aagaaaaatg
     2101 ataatataaa aataaagtaa gaacgcatta aagtaactaa agttatttat tgtatttttt
     2161 aattctccga aaaaatttaa taaataaata gaatcttgta ataataactg agcataatct
     2221 ttgcaatttt taatcttgaa taatatcctt taagtatagc cagcaatcaa aagtaaatag
     2281 agaataagta agcaattgtt atttcagtat cagactggca ataaaacgcc gttatgatcc
     2341 agcaatgttt ggtagcgctt tatttactga tgctatgcta actgttcgct atctatactg
     2401 tgattgtcgg tagagttatt tacagggtaa aaataagttt ataggaggtg gaaaatgaat
     2461 aaggggttaa aaataatcgg tgttatcatg agtttactgc tactttcagt atttctagcg
     2521 attgcgttga tcaatgcgcc gtatattatg cctgaacaat tggaacgatt tcgcttcttt
     2581 actttaacga attactacat gcaacaatat attttctggg cagctgtagt atttgccgtt
     2641 cttgctatta tcatcttgct agtcgttctt ttctatccaa agtcaagagg aactttcgtt
     2701 atgaaacggg aagatggcaa actaacaatc gacaaaaaag cgatcgaagg gttggtacgt
     2761 tcccatttac acgaagaaga gttcattcat tcacctaaag tacggatacg ttcaacgaaa
     2821 aatcgaatcc atatccatgt caacggagac ttgaaacgaa cttcttcatt agtagggaaa
     2881 acaggtgtat tgatgcaaga tatcgaagaa gaagtaacta aagtactcgg aacaaaagaa
     2941 acggtcaaag tggcagtcac atacagcggt tatgaagaac aagaagatac aagagactac
     3001 aagcactctc gagtggaata ggagggccaa ataatgaaag aactattttc agcatacaag
     3061 ctccctatta tctttggggg tcttggacta ctgctagctg ttttatggtt aacgattgga
     3121 tttttcaaga cattgttgat cataatcatg acagcaattg gcgcaggaat cggtttttat
     3181 ttgaaacagt caggaatagt tgatcaattt tttaaatcat aggaggaaac aaagatggaa
     3241 aacaaaaaca tgaatacaac aaatggatca gttttggcaa atgaaaaagg agaaaaccgc
     3301 atgatgaatg gtgaattgac ttatgaagac aaagtcatcc aaaaaatcat cggttatgca
     3361 ttgaaagatg tagatggtct attgacatta gatggcggat ttttctcaaa tatggctgaa
     3421 aaattagtca acacagataa cgtaacagca ggtatcaata cagaagtagg taagaaacaa
     3481 gtagctgtgg atctagatgt gatcgtagaa tacggtaaag acatcgaaga tatttacaac
     3541 caaatcaaag aattgatcag tactgaagta aacaatatga cacatttaga tgttatcgaa
     3601 gtgaatgtga atgttgcaga catcaaaaca caagaagaat accaaaaaga tcaagaaaca
     3661 gtacaagaca aagtaacaga agcagcaaaa tccacaggac aatttgcttc acgccaaaca
     3721 gataaagcaa aatctgctgt aggcaaaggt gcacaaaaag tcaaagaaaa taacgaacca
     3781 cgtgtacaat aagaagtaag aagtcagtta ggaggaattt aaaatgggat ttatttgggc
     3841 attaatcgtc ggcggtatca ttggagcaat tgcaggagca atcacgaata aaggatcatc
     3901 aatggggatc atcgcaaaca tcattgctgg tttagtaggt tcagcaattg gacaagcttt
     3961 actaggaact tggggaccaa caattgcagg aatggctatc attccatcat tgatcggtgc
     4021 aatcatttta gtattagtcg tttcattctt tgcacgtaaa ttataagcaa attgaagaaa
     4081 aacttatgtt gaaatgaaga acaaatttga gaaccgtagc gacactcgct gcggttcttt
     4141 tttatatatt ttatatgaag tattctttat tccttatgaa aaaatatctt taaaaataaa
     4201 aaaagacgaa aataaacggt tttctataaa aagaagaccg tttatttttt gtctttattt
     4261 gtgtgaaaaa cgaaaaagag cacaacacat caagctttta ttaattgtct gtcttttata
     4321 aatgcaagta ttaattcaaa aaaacagaag aaaaaaacaa ctacaccttc ttttatactc
     4381 gtaatcgtag aaaggaggcg aaaaaaatga atacattacc tatcaataca ggagatacag
     4441 cattcatgat tttatgtaca gcaatggttt gcctgatgac tcctggtcta gctttttttt
     4501 acggcggatt agcacggaag aataatatac taacgatcat gtctcaatct tttctctccg
     4561 ttgggataac aacgattatg tgggtatttg gtggattcgg attggccttt ggacgggata
     4621 tcggcggtgt tattggaaac ccggcagatt tctttttgat gagacatgtt acaagcttcc
     4681 ccaacatgtt tcatggtgca acgattccat ttttaatgtt ctttctttac caactgatgt
     4741 ttatcgtgat taccgttcct ttgatgacag gagcttttgc agggagattg aatttaaaag
     4801 gctatattat acttgttatt ttttggaatc tgctgattta tttccctgtt tgccactgga
     4861 tatggggtgg cggatttttg gatcagatgg gatttagaga tttcgctggt ggagcagtca
     4921 tccacacgac agctggattc ggctctttag catgtatcct cacacttggt caaagaaaga
     4981 ttcctttagg agaaagaagt cagcatagca gtcttatggc agctgctatc ggtacaggac
     5041 tgctttggtt tggctggttc ggttttaatt ccggtggtgc acttcgagca gatgaacaag
     5101 cagtcaatgc atttggttca acattcatcg ctcttgcatt tgcgatgatt acttggctga
     5161 tcattgcaaa agtaaaagga aatggctttg attttgtcga cgtactaaca ggatccgttg
     5221 ctggattggc agccattacg ccttgtgccg gttatgtaga agcaaaaagt gccatgctga
     5281 ttgggatcat tgcgggtatc gtctgtcatg ctgctgttga ttttcgtaaa aagaaacaat
     5341 gggatgatgc acttgatgtg tggggtgttc atggcatggg cggattcaca ggtacgattc
     5401 tgatagggat ctttgcatct aacagccatt tactaacgaa tccaagcagc tggtacttct
     5461 tagggattca agtgattggt gtcatcatta ctgccgtgta tgcctatgtg ctgacaacag
     5521 tgattttaaa atcggcgatg cactttacaa cgatcactac tacaaaagaa gaacaagaag
     5581 aaggattgga ctttttcttc tttggctttt tggctatgac catctcaatg gtcgtttcat
     5641 tatatgaata tcccacattc gcgacctcag gcttcagctt agtatttttc ttgttattag
     5701 gcggtttgct atggtttatc cctgtggcat tgtgcgcagc agagatggca acggtgaaag
     5761 gatgggaaaa aggtgtttcc caaacaaaaa gatattaaga aacagcccat gtttacctgc
     5821 ttgcaaacaa aagaatattg ctactagact aagagatact acataagtgt gttggtatct
     5881 cttttttgtt gccgtgaaac agcagactat ttcatttctg cttgattacc ttaaaactta
     5941 ttagtctatg gtttgtcaag ggcttgttgc gtgagaaatg ctgaatatca ccctttacaa
     6001 aacataggct gataagtaac ttttcaaaag cagaaaagaa atggcaatgc ttataacaat
     6061 atgatataat gcagatgaag acaaaaaagg aatttgtcga gctggttagt tcgagataat
     6121 tttgagtttg ttaagatgtg gtgcacttga tggaatcaca ggtacgacat catattatgg
     6181 aggatgaaat agatgatagg tttgattgtt gcgaggtcaa agaataatgt tataggcaag
     6241 aatggtaata taccatggaa aataaaggga gaacaaaagc aatttagaga gttaacaacg
     6301 ggtaatgtgg ttattatggg gcgaaagtct tatgaagaaa tcggtcatcc gttgcctaat
     6361 agaatgaata ttgttgtttc caccacaaca gagtatcaag gagataattt agtttcagtt
     6421 aaatcattag aagatgcatt attattggct aaaggacgag atgtatacat atctggtgga
     6481 tatggactat ttaaggaagc tttgcaaata gtagataaaa tgtatatcac agaagtagat
     6541 ttaaatattg aagatggaga tacattcttt ccagaatttg atatcaatga ttttgaagtt
     6601 ttgatagggg aaacacttgg tgaggaagtg aaatatacga gaacatttta tgtaaggaaa
     6661 aaatgaattg agtaaatttt ggatttaggg tgttttcata aatatattgc tttttatgca
     6721 tatataaatt gttgtaagac aaattccagt ttgtatgggg cgataaaatc agaatttgag
     6781 agaggattga atatgaat
//
LOCUS       DAZUKT010000278         5984 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.137, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000278 DAZUKT010000000
VERSION     DAZUKT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.137"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..210
                     /locus_tag="ACG816_003258"
                     /pseudo
     CDS             46..210
                     /locus_tag="ACG816_003258"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(295..3219)
                     /locus_tag="ACG816_003259"
     CDS             complement(295..3219)
                     /locus_tag="ACG816_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS4922032.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDSMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3365..3940
                     /locus_tag="ACG816_003260"
     CDS             3365..3940
                     /locus_tag="ACG816_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4922033.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4154..4849
                     /gene="vanR-A"
                     /locus_tag="ACG816_003261"
     CDS             4154..4849
                     /gene="vanR-A"
                     /locus_tag="ACG816_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4922034.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4827..5978
                     /gene="vanS"
                     /locus_tag="ACG816_003262"
     CDS             4827..5978
                     /gene="vanS"
                     /locus_tag="ACG816_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS4922035.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPGFCCKVLNKE"
BASE COUNT         1852 a         1131 c         1134 g         1867 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct tttggggtac cctcaaggtg
       61 gagatgttta acctggatac gtttgacaga ccagcgtact tagaccggaa aatcaaggca
      121 tacatcgcgt tcttcaacaa tgaacgcgtt actttggata tgggattagc aattcctacg
      181 gaagagaaga ttctacaaat gattgcataa acgctacaaa aaaagaccac acatttttac
      241 atgcatggtc taaaatactt tgttttttta cctgtctact tgacaggggg cagttcactc
      301 tgagttaaaa tggtattctc ctagtaaatt aatatgttcc caacctaagg gcgacatatg
      361 gtgtaacaaa tcttcattaa agctacctgt ccgtttttta tattcaactg ctgttgttag
      421 gtggagagta ttccaaatac ttatagcatt gataattatg tttaaagcac tggctctttg
      481 caattgatgc tgtatggtgc gttctctaag ctcaccttgt tttccgaaga aaatagctct
      541 tgccaatcca ttcatggctt ctcctttatt caatcctctt tgtatttttc ttcttaatga
      601 ttcatccgat atataattca aaataaagat cgttttttct attcggccca tctcacgtaa
      661 ggctgtagct aagctgtttt gtcttgaata ggaacctagc ttccccataa taagggatgc
      721 tgaaactgtt ccctccctta tagaatgagc taatcgcaaa acatcctcat aattttcttt
      781 aatgaccttt gtatttattt gtccacgtaa aatggcttct agttttggat actcacttgc
      841 tttatctatc gtaaataatt ttgagtccga taaatccctt attcttgggg caaatttaaa
      901 tcctaataaa tgagtcagtc cgaatatttg gtcagtgtaa ccggcagtgt ctgtataatg
      961 ttcctctatg tttagatccg tctcatgatg taacaaacca tccaaaacat gaatcgcatc
     1021 tcttgaatta gtatgaataa tctttgtgta gtaagaagag aattgatcac ttgtaaatcg
     1081 gtagatggtg gctccttttc cagttccata atgtggattt gcatctgcat gtagtgatga
     1141 aacacctagc tgcattctca taccatctga cgaagatgtt gtaccgtcgc cccaatagaa
     1201 aggcaattgt aatttatgat gaaagtttac taatatggct tgggctttat tcatggaatc
     1261 ttcatacatg cgccattgag atacattggc tagttgctta tatgtaagtc cgggtgtggc
     1321 ttcggccatc ttgctcaagc caatattcat tcccattcct aaaagggcag ccatgataat
     1381 gattgtttct tccttatctg gttttcgatt attggaagca tgagtgaatt gctcatgaaa
     1441 tcctgttata tgggccacat ccatgagtaa atcagttaat tttattcttg gtagcatctg
     1501 ataaaggctt gcactaaatt tttttgcttc ttctggaaca tctttttcta agcgtgcaag
     1561 tgatagcttt cctttttcaa gagaaacccc atctaactta ttggaattgg cagctaacca
     1621 ctttaacctt tcattaaagc tgctggttct ctccgttata taatcttcga atgataaact
     1681 aactgataat ctcgtattcc ccttcgattg attccatgta tcttccgaaa acaaatattc
     1741 ctcaaaatcc ctatattgtc tgctgccaac aatggaaaca tctcctgccc gaacatgctc
     1801 ccgaagttct gttaaaacag ccatttcata gtaatgacga ttaattgttg taccatcatc
     1861 ctcgtataaa tgtcttttcc atcgttttga aataaaatcc acaggtgagt catcaggcac
     1921 ttttcgcttt ccagattcgt tcattcctcg gataatctca acagcttgta aaagtggctc
     1981 atttgccttt gtagaatgaa attccaatac tcttaatagc gttggcgtat attttcttag
     2041 tgaataaaac cgtttttgca gtaagtctaa ataatcatag tcggcaggac gtgcaagttc
     2101 ctgagcctct tctactgaag agacaaaggt attccattca ataaccgatt ctaaaacctt
     2161 aaaaacgtct aatttttcct ctcttgcttt aattaatgct tgtccgatgt tcgtaaagtg
     2221 tataactttc tcatttagct ttttaccgtt ttgtttctgg atttcctctt gagccttacg
     2281 accttttgat aacaaactaa gtatttgcct atcatgaatt tcaaacgctt tatccgttag
     2341 ctcctgagta agttgtaata aatagatggt taatatcgaa taacgtttat tttcttgaaa
     2401 gtcacggaat gcatacggct cgtatcttga gcctaagcga gacagctgca acaggcggtt
     2461 acggtgcaaa tgactaattt gcactgtttc taaatccatt cctcgtatgt attcgagtcg
     2521 ttctattatt tttagaaaag tttcgggtga aggatgaccc ggtggctctt ttaaccaacc
     2581 caatatcgtt ttattggatt cggatggatg ctgcgaggta ataatccctt caagcttttc
     2641 tttttgctca tttgttagag atttactaac cgtattaaat agcttctttt cagccattgc
     2701 ccttgcttcc cacaccattc tttcaagtgt agtgatagca ggcagtataa ttttgttttt
     2761 tcttagaaaa tctatgcatt catgcagtag atgaatggca tcaccatttt ccaaagctaa
     2821 ttgatgaagg tacttaaatg tcattcgata ttcactcagg gtaaaagtta caaagtcgta
     2881 ttcacttcga atttctttca aatgatccca aagtgtattt tccctttgag gataatgatc
     2941 aagcgaggat ggactaacac caatctgttt cgatatatat tgtatgaccg aatctgggat
     3001 gcttttgata tgagtgtatg gccaaccggg ataccgaaga acagctaatt gaacagcaaa
     3061 tcctaaacgg ttttcttccc tccttcgctt attaactatt tctaaatccc gtttggaaaa
     3121 agtgaagtag gtccccagta tccattcatc ttcagggatt tgcataaaag cctgtctctg
     3181 ttccggtgta agcaattctc tacctctcgc aattttcatt cagtatcatt ccatttctgt
     3241 attttcaatt tattagttca attatatatc aatagagtgt actctattga tacaaatgta
     3301 gtagactgat aaaatcatag ttaagagcgt ctcataagac ttgtctcaaa aatgaggtga
     3361 tattttgcgg aaaatcggtt atattcgtgt cagttcgact aaccagaatc cttcaagaca
     3421 atttcagcag ttgaacgaga tcggaatgga tattatatat gaagagaaag tttcaggagc
     3481 aacaaaggat cgcgagcaac ttcaaaaagt gttagacgat ttacaggaag atgacatcat
     3541 ttatgttaca gacttaactc gaatcactcg tagtacacaa gatctatttg aattaatcga
     3601 taacatacga gataaaaagg caagtttaaa atcactaaaa gatacatggc ttgatttatc
     3661 agaagataat ccatacagcc aattcttaat tactgtaatg gctggtgtta accaattaga
     3721 gcgagatctt attcggatga gacaacgtga agggattgaa ttggctaaga aagaaggaaa
     3781 gtttaaaggt cgattaaaga agtatcataa aaatcacgca ggaatgaatt atgcggtaaa
     3841 gctatataaa gaaggaaata tgactgtaaa tcaaatttgt gaaattacta atgtatctag
     3901 ggcttcatta tacaggaaat tatcagaagt gaataattag ccattctgta ttccgctaat
     3961 gggcaatatt tttaaagaag aaaaggaaac tataaaatat taacagcctc ctagcgatgc
     4021 cgaaaagccc tttgataaaa aaagaatcat catcttaaga aattcttagt catttattat
     4081 gtaaatgctt ataaattcgg ccctataatc tgataaatta ttaagggcaa acttatgtga
     4141 aagggtgata actatgagcg ataaaatact tattgtggat gatgaacatg aaattgccga
     4201 tttggttgaa ttatacttaa aaaacgagaa ttatacggtt ttcaaatact ataccgccaa
     4261 agaagcattg gaatgtatag acaagtctga gattgacctt gccatattgg acatcatgct
     4321 tcccggcaca agcggcctta ctatctgtca aaaaataagg gacaagcaca cctatccgat
     4381 tatcatgctg accgggaaag atacagaggt agataaaatt acagggttaa caatcggcgc
     4441 ggatgattat ataacgaagc cctttcgccc actggagtta attgctcggg taaaggccca
     4501 gttgcgccga tacaaaaaat tcagtggagt aaaggagcag aacgaaaatg ttatcgtcca
     4561 ctccggcctt gtcattaatg ttaacaccca tgagtgttat ctgaacgaga agcagttatc
     4621 ccttactccc accgagtttt caatactgcg aatcctctgt gaaaacaagg ggaatgtggt
     4681 tagctccgag ctgctatttc atgagatatg gggcgacgaa tatttcagca agagcaacaa
     4741 caccatcacc gtgcatatcc ggcatttgcg cgaaaaaatg aacgacacca ttgataatcc
     4801 gaaatatata aaaacggtat ggggggttgg ttataaaatt gaaaaataaa aaaaacgact
     4861 attccaaact agaacgaaaa ctttacatgt atatcgttgc aattgttgtg gtagcaattg
     4921 tattcgtgtt gtatattcgt tcaatgatcc gagggaaact tggggattgg atcttaagta
     4981 ttttggaaaa caaatatgac ttaaatcacc tggacgcgat gaaattatat caatattcca
     5041 tacggaacaa tatagatatc tttatttatg tggcgattgt cattagtatt cttattctat
     5101 gtcgcgtcat gctttcaaaa ttcgcaaaat actttgacga gataaatacc ggcattgatg
     5161 tacttattca gaacgaagat aaacaaattg agctttctgc ggaaatggat gttatggaac
     5221 aaaagctcaa cacattaaaa cggactctgg aaaagcgaga gcaggatgca aagctggccg
     5281 aacaaagaaa aaatgacgtt gttatgtact tggcgcacga tattaaaacg ccccttacat
     5341 ccattatcgg ttatttgagc ctgcttgacg aggctccaga catgccggta gatcaaaagg
     5401 caaagtatgt gcatatcacg ttggacaaag cgtatcgact cgaacagcta atcgacgagt
     5461 tttttgagat tacacggtat aacctacaaa cgataacgct aacaaaaacg cacatagacc
     5521 tatactatat gctggtgcag atgaccgatg aattttatcc tcagctttcc gcacatggaa
     5581 aacaggcggt tattcacgcc cccgaggatc tgaccgtgtc cggcgaccct gataaactcg
     5641 cgagagtctt taacaacatt ttgaaaaacg ccgctgcata cagtgaggat aacagcatca
     5701 ttgacattac cgcgggcctc tccggggatg tggtgtcaat cgaattcaag aacactggaa
     5761 gcatcccaaa agataagcta gctgccatat ttgaaaagtt ctataggctg gacaatgctc
     5821 gttcttccga tacgggtggc gcgggacttg gattggcgat tgcaaaagaa attattgttc
     5881 agcatggagg gcagatttac gcggaaagca atgataacta tacgacgttt agggtagagc
     5941 ttccagggtt ctgttgcaaa gttttaaata aagaataaaa tccc
//
LOCUS       DAZUKT010000279         5851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.140, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000279 DAZUKT010000000
VERSION     DAZUKT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.140"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..1014
                     /locus_tag="ACG816_003263"
     CDS             322..1014
                     /locus_tag="ACG816_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4922036.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG816_003264"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG816_003264"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4922037.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="ACG816_003265"
     CDS             complement(1910..3862)
                     /locus_tag="ACG816_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4922038.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="ACG816_003266"
     CDS             complement(3869..4105)
                     /locus_tag="ACG816_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4922039.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="ACG816_003267"
     CDS             4507..4797
                     /locus_tag="ACG816_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4922040.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(5069..5418)
                     /locus_tag="ACG816_003268"
                     /pseudo
     CDS             complement(5069..5418)
                     /locus_tag="ACG816_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5511..5813
                     /locus_tag="ACG816_003269"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="ACG816_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1997 a          925 c         1010 g         1919 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca t
//
LOCUS       DAZUKT010000280         3014 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.180, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000280 DAZUKT010000000
VERSION     DAZUKT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.180"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1300
                     /locus_tag="ACG816_003270"
                     /note="aph(2'')-Ia"
     CDS             <1..1300
                     /locus_tag="ACG816_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4922041.1"
                     /translation="KHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKT
                     DEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQ
                     KSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEI
                     IGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYI
                     SDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID
                     NKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLL
                     LDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKE
                     YQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1430..2602)
                     /locus_tag="ACG816_003271"
     CDS             complement(1430..2602)
                     /locus_tag="ACG816_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFS4922042.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1053 a          388 c          537 g         1036 t
ORIGIN      
        1 aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa
       61 tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca
      121 ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta
      181 ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga
      241 aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc
      301 ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg
      361 caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa
      421 ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat
      481 aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac
      541 aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt
      601 aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga
      661 tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta
      721 ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag
      781 acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa
      841 tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga
      901 aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa
      961 aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag
     1021 attaactgga ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat
     1081 atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag
     1141 aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata
     1201 ttatcctatt gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa
     1261 tggtagaaaa gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc
     1321 tattataaaa gacattagta ttaaatagtt taaaaaaatg aaaaataata aaggaagtga
     1381 gtcaagtcca gactcctgtg taaaatgcta tacaatgttt ttaccatttc tacttatcaa
     1441 aattgatgta ttttcttgaa gaataaatcc attcatcatg taggtccata agaacggctc
     1501 caattaagcg attggctgat gtttgattgg ggaagatgcg aataatcttt tctcttctgc
     1561 gtacttcttg attcagtcgt tcaattagat tggtactctt tagtcgattg tgggaatttc
     1621 cttgtacggt atattgaaag gcgtcttcga atccatcatc caatgatgcg caagcttttg
     1681 aatattttgg ttgatcgata taatcatgaa tcaatcgatt tttagcctca cgcgctaagt
     1741 taatatctgt gaacttaaaa attcctttaa cagcttctct gaaagatttt gaattttttt
     1801 taggaatggt ggtaaagata tttcttagga agtgaacttg gcatctttgc caacttacgt
     1861 tggtgaagga ttttctaatg gcagagacta atcctttgtg cgcatcagaa ataacgagtt
     1921 ccgtaccttg taaaccgcgt tcttttaggt attcaaaaaa tgttgtccag gtctcttcgc
     1981 tttcgccact ttgaatcatg aagccgataa tttcacggtc gccatctttg gttattccaa
     2041 tcgctatatg acagcttttt gagagtactc gattttcttc tcgtactttt atatagagta
     2101 catcggtcat taagtaagga taattttttt ctgataataa acgattctgc cactcgttaa
     2161 ccataggttc tagctgttct gttaagctag aaacgaagga cttagagacg gatttaccac
     2221 aaagttcttc cacaattttt gatactttac gagttgaaac gcctgataca tacatttcca
     2281 acattgaagc catgagggct ttttcgtttc gttgataacg ttcaaacact gtgggtgaaa
     2341 aatggccatc acgtgttctg ggtactttta attctagcgt gcctacacgt gtcgtaaagc
     2401 tgcgctcata atagccattt cgttgacttt gtcggttttc tgttcgttca tattcttttg
     2461 cttgaatata ttctgttcgt tgattttcca ttagttgatt aaataccgtt gttaaaatat
     2521 ttttagaaac gtcatccttt acagaatatt caataatgct ttgaatctct tcgcttttca
     2581 gtgtaaaatg tacttgggtc atgtaaaagt cctcctgggt atgtttttgt cgttaaaaac
     2641 attgtaccgt aaaaggactg ttatatggcc tttttacttt tacacaatta tacggacttt
     2701 atctatataa aggcgctgtc agagtttttt gacttagaac atacattcat tcaagagact
     2761 ttaaacaagc tagcagaacg cccaaaaacg gacacacaac tcgatttgtt tagctatgat
     2821 acaggctgaa aataaaaccc gcactatgcc attacattta tatctatgat acgtgtttgt
     2881 ttttctgtac tgtttcctga gtgattagca gaaatataaa gattaaaatt ttaattgatt
     2941 attaggaaga gaaggagaat gtatcccgaa aactttttag ttggcttgga ctgaacgaag
     3001 tgagggaaag gcta
//
LOCUS       DAZUKT010000281         2976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.184, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000281 DAZUKT010000000
VERSION     DAZUKT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.184"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            297..1265
                     /locus_tag="ACG816_003272"
     CDS             297..1265
                     /locus_tag="ACG816_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4922043.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1258..2289
                     /gene="vanA"
                     /locus_tag="ACG816_003273"
     CDS             1258..2289
                     /gene="vanA"
                     /locus_tag="ACG816_003273"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4922044.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2295..2903
                     /locus_tag="ACG816_003274"
     CDS             2295..2903
                     /locus_tag="ACG816_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS4922045.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          885 a          569 c          719 g          803 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg cttccagcga tgccagactt
       61 ggttgataaa aggaggtcct aagagatgta tataattttt taggaaaatc tcaaggttat
      121 ctttactttt tcttaggaaa ttaacaattt aatattaaga aacggctcgt tcttacacgg
      181 tagacttaat accgtaagaa cgagccgttt tcgttcttca gagaaagatt tgacaagatt
      241 accattggca tccccgtttt atttggtgcc tttcacagaa agggttggtc ttaattatga
      301 ataacatcgg cattactgtt tatggatgtg agcaggatga ggcagatgca ttccatgctc
      361 tttcgcctcg ctttggcgtt atggcaacga taattaacgc caacgtgtcg gaatccaacg
      421 ccaaatccgc gcctttcaat caatgtatca gtgtgggaca taaatcagag atttccgcct
      481 ctattcttct tgcgctgaag agagccggtg tgaaatatat ttctacccga agcatcggct
      541 gcaatcatat agatacaact gctgctaaga gaatgggcat cactgtcgac aatgtggcgt
      601 actcgccgga tagcgttgcc gattatacta tgatgctaat tcttatggca gtacgcaacg
      661 taaaatcgat tgtgcgctct gtggaaaaac atgatttcag gttggacagc gaccgtggca
      721 aggtactcag cgacatgaca gttggtgtgg tgggaacggg ccagataggc aaagcggtta
      781 ttgagcggct gcgaggattt ggatgtaaag tgttggctta tagtcgcagc cgaagtatag
      841 aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag cgatatcgtt acgcttcatg
      901 tgccgctcaa tacggatacg cactatatta tcagccacga acaaatacag agaatgaagc
      961 aaggagcatt tcttatcaat actgggcgcg gtccacttgt agatacctat gagttggtta
     1021 aagcattaga aaacgggaaa ctgggcggtg ccgcattgga tgtattggaa ggagaggaag
     1081 agtttttcta ctctgattgc acccaaaaac caattgataa tcaattttta cttaaacttc
     1141 aaagaatgcc taacgtgata atcacaccgc atacggccta ttataccgag caagcgttgc
     1201 gtgataccgt tgaaaaaacc attaaaaact gtttggattt tgaaaggaga caggagcatg
     1261 aatagaataa aagttgcaat actgtttggg ggttgctcag aggagcatga cgtatcggta
     1321 aaatctgcaa tagagatagc cgctaacatt aataaagaaa aatacgagcc gttatacatt
     1381 ggaattacga aatctggtgt atggaaaatg tgcgaaaaac cttgcgcgga atgggaaaac
     1441 gacaattgct attcagctgt actctcgccg gataaaaaaa tgcacggatt acttgttaaa
     1501 aagaaccatg aatatgaaat caaccatgtt gatgtagcat tttcagcttt gcatggcaag
     1561 tcaggtgaag atggatccat acaaggtctg tttgaattgt ccggtatccc ttttgtaggc
     1621 tgcgatattc aaagctcagc aatttgtatg gacaaatcgt tgacatacat cgttgcgaaa
     1681 aatgctggga tagctactcc cgccttttgg gttattaata aagatgatag gccggtggca
     1741 gctacgttta cctatcctgt ttttgttaag ccggcgcgtt caggctcatc cttcggtgtg
     1801 aaaaaagtca atagcgcgga cgaattggac tacgcaattg aatcggcaag acaatatgac
     1861 agcaaaatct taattgagca ggctgtttcg ggctgtgagg tcggttgtgc ggtattggga
     1921 aacagtgccg cgttagttgt tggcgaggtg gaccaaatca ggctgcagta cggaatcttt
     1981 cgtattcatc aggaagtcga gccggaaaaa ggctctgaaa acgcagttat aaccgttccc
     2041 gcagaccttt cagcagagga gcgaggacgg atacaggaaa cggcaaaaaa aatatataaa
     2101 gcgctcggct gtagaggtct agcccgtgtg gatatgtttt tacaagataa cggccgcatt
     2161 gtactgaacg aagtcaatac tctgcccggt ttcacgtcat acagtcgtta tccccgtatg
     2221 atggccgctg caggtattgc acttcccgaa ctgattgacc gcttgatcgt attagcgtta
     2281 aaggggtgat aagcatggaa ataggattta cttttttaga tgaaatagta cacggtgttc
     2341 gttgggacgc taaatatgcc acttgggata atttcaccgg aaaaccggtt gacggttatg
     2401 aagtaaatcg cattgtaggg acatacgagt tggctgaatc gcttttgaag gcaaaagaac
     2461 tggctgctac ccaagggtac ggattgcttc tatgggacgg ttaccgtcct aagcgtgctg
     2521 taaactgttt tatgcaatgg gctgcacagc cggaaaataa cctgacaaag gaaagttatt
     2581 atcccaatat tgaccgaact gagatgattt caaaaggata cgtggcttca aaatcaagcc
     2641 atagccgcgg cagtgccatt gatcttacgc tttatcgatt agacacgggt gagcttgtac
     2701 caatggggag ccgatttgat tttatggatg aacgctctca tcatgcggca aatggaatat
     2761 catgcaatga agcgcaaaat cgcagacgtt tgcgctccat catggaaaac agtgggtttg
     2821 aagcatatag cctcgaatgg tggcactatg tattaagaga cgaaccatac cccaatagct
     2881 attttgattt ccccgttaaa taaactttta accgttgcac ggacaaacta tataagcggt
     2941 tctgttgcaa agttttaaat aaagaataaa atccct
//
LOCUS       DAZUKT010000282         2772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.187, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000282 DAZUKT010000000
VERSION     DAZUKT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.187"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG816_003275"
     CDS             111..194
                     /locus_tag="ACG816_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4922046.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG816_003276"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG816_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4922047.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACG816_003277"
     CDS             complement(1319..2182)
                     /locus_tag="ACG816_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4922048.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACG816_003278"
     CDS             complement(2184..2456)
                     /locus_tag="ACG816_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4922049.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACG816_003279"
     CDS             complement(2473..2682)
                     /locus_tag="ACG816_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4922050.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DAZUKT010000283         2054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.200, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000283 DAZUKT010000000
VERSION     DAZUKT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.200"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            216..1127
                     /locus_tag="ACG816_003280"
     CDS             216..1127
                     /locus_tag="ACG816_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFS4922051.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1280..1765
                     /gene="vanZ-A"
                     /locus_tag="ACG816_003281"
     CDS             1280..1765
                     /gene="vanZ-A"
                     /locus_tag="ACG816_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS4922052.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          726 a          288 c          390 g          650 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgt atcacttatg aaaattcatc
       61 tacattggtg ataatagtaa atccagtagg gcgaaataat tgactgtaat ttacggggca
      121 aaacggcaca atctcaaacg agattgtgcc gtttaagggg aagattctag aaatatttca
      181 tacttccaac tatatagtta aggaggagac tgaaaatgaa gaagttgttt tttttattgt
      241 tattgttatt cttaatatac ttaggttatg actacgttaa tgaagcactg ttttctcagg
      301 aaaaagtcga atttcaaaat tatgatcaaa atcccaaaga acatttagaa aatagtggga
      361 cttctgaaaa tacccaagag aaaacaatta cagaagaaca ggtttatcaa ggaaatctgc
      421 tattaatcaa tagtaaatat cctgttcgcc aagaaagtgt gaagtcagat atcgtgaatt
      481 tatctaaaca tgacgaatta ataaatggat acgggttgct tgatagtaat atttatatgt
      541 caaaagaaat agcacaaaaa ttttcagaga tggtcaatga tgctgtaaag ggtggcgtta
      601 gtcattttat tattaatagt ggctatcgag actttgatga gcaaagtgtg ctttaccaag
      661 aaatgggggc tgagtatgcc ttaccagcag gttatagtga gcataattca ggtttatcac
      721 tagatgtagg atcaagcttg acgaaaatgg aacgagcccc tgaaggaaag tggatagaag
      781 aaaatgcttg gaaatacggg ttcattttac gttatccaga ggacaaaaca gagttaacag
      841 gaattcaata tgaaccatgg catattcgct atgttggttt accacatagt gcgattatga
      901 aagaaaagaa tttcgttctc gaggaatata tggattacct aaaagaagaa aaaaccattt
      961 ctgttagtgt aaatggggaa aaatatgaga tcttttatta tcctgttact aaaaatacca
     1021 ccattcatgt gccgactaat cttcgttatg agatatcagg aaacaatata gacggtgtaa
     1081 ttgtgacagt gtttcccgga tcaacacata ctaattcaag gaggtaagga tggcggaatg
     1141 aaaccaacga aattaatgaa cagcattatt gtactagcac ttttggggta acgttagctt
     1201 tttaatttaa aacccacgtt aactaggaca ttgctatact aatgatacaa cttaaacaaa
     1261 agaattagag gaaattatat tgggaaaaat attatctaga ggattgctag ctttatattt
     1321 agtgacacta atctggttag tgttattcaa attacaatac aatattttat cagtatttaa
     1381 ttatcatcaa agaagtctta acttgactcc atttactgct actgggaatt tcagagagat
     1441 gatagataat gttataatct ttattccatt tggcttgctt ttgaatgtca attttaaaga
     1501 aatcggattt ttacctaagt ttgcttttgt actggtttta agtcttactt ttgaaataat
     1561 tcaatttatc ttcgctattg gagcgacaga cataacagat gtaattacaa atactgttgg
     1621 aggctttctt ggactgaaat tatatggttt aagcaataag catatgaatc aaaaaaaatt
     1681 agacagagtt attatttttg taggtatact tttgctcgta ttattgctcg tttaccgtac
     1741 ccatttaaga ataaattacg tgtaagatgt ctaaatcaag caatctgatc tttcatacac
     1801 ataaagatat tgaatgaatt ggattagatg gaaaacggga tgtggggaaa ctcgcccgta
     1861 ggtgtgaagt gaggggaaaa ccggtgataa agtaaaaagc ttacctaaca ctatagtaac
     1921 aaagaaagcc caattatcaa ttttagtgct gaggaattgg tctctttaat aaatttcctt
     1981 aacgttgtaa atccgcattt tcctgacggt acccccaaac taagatttca tagatagatt
     2041 ttctgataac ttta
//
LOCUS       DAZUKT010000284         1781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5246
            SAMEA115707596-rid22580443.guided.205, whole genome shotgun
            sequence.
ACCESSION   DAZUKT010000284 DAZUKT010000000
VERSION     DAZUKT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707596
            Sequence Read Archive: ERR13197324
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288082.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288082.1
            Genome Coverage       :: 185x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:14:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,283
            CDSs (total)                      :: 3,220
            Genes (coding)                    :: 3,130
            CDSs (with protein)               :: 3,130
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 41 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 21 of 90
            Pseudo Genes (multiple problems)  :: 17 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707596-rid22580443.guided.205"
                     /isolate="VRE5246"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACG816_003282"
     CDS             24..416
                     /locus_tag="ACG816_003282"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4922053.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG816_003283"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG816_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFS4922054.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
